YSI 6600 Sonde
Instruction Manual
Version 1
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Contents
YSI 6600 Sonde...........................................................................................4 Introduction - What is a Sonde?.....................................................................................4 What parameters does the sonde measure?.................................................................4 Important Points to Remember......................................................................................5 Initial Setup.....................................................................................................................6 Installin !atteries in the Sonde.............................................................................6 Installin Sensors...................................................................................................." #ptical Sensors...................................................................................................$ Reular Sensors..................................................................................................$ Installin Probe %uard or &alibration &up............................................................10
YSI 650 '(S (ispla) *nit..................................... *nit........................................................ ..................................... .................. 11 Installin !atteries in the 650 (ispla) *nit...................................................................11 &onnectin the Sonde to the YSI 650 (ispla) *nit.....................................................11 Initial Setup........... Setup.................... ................. ................. .................. .................. .................. .................. .......................... .........................................1+ ........................1+ &ommunication &ommunication bet,een the 650 displa) unit and the sonde.................................15 'ain 'enu................................................................................................................16 S)stem setup........................................................................................................16 (ate and ime...................................................................................................16 !arometer &alibration.......................................................................................1" he Po,er Sonde #ption..................................................................................1 Sonde menu..........................................................................................................1$
&alibratin Sonde Sensors..................... Sensors........................................ ...................................... ................................. ..............1$ 1$ &alibratin a clean or ne, sonde................................................................................./0 Supplies reuired....................................................................................................../0 &alibration Instructions............................................................................................./0 1. (issoled #2)en............................................................................................./0 /. (epth.......... (epth................... .................. ................. ................. .................. .................. .................. .......................... ......................................../1 ......................./1 +. &onductiit) and emperature........................................................................../1 4. p3....................................................................................................................../+ 5. #2idation Reduction Potential #RP.............................................................../4 6. &hloroph)ll......................................................................................................../5 ". urbidit)............................................................................................................/6 &alibration Sheet....................................................................................................../ oin data 7 settin up the calibrated sonde to be deplo)ed................................../$
89ter a Sonde (eplo)ment 7 Retriein the sonde: do,nloadin the data and chec;in 9or dri9t.................. d ri9t..................................... ...................................... ...................................... .......................... .......+0 +0 Sonde retrieal.............................................................................................................+0 (o,nloadin Water
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Contents
YSI 6600 Sonde...........................................................................................4 Introduction - What is a Sonde?.....................................................................................4 What parameters does the sonde measure?.................................................................4 Important Points to Remember......................................................................................5 Initial Setup.....................................................................................................................6 Installin !atteries in the Sonde.............................................................................6 Installin Sensors...................................................................................................." #ptical Sensors...................................................................................................$ Reular Sensors..................................................................................................$ Installin Probe %uard or &alibration &up............................................................10
YSI 650 '(S (ispla) *nit..................................... *nit........................................................ ..................................... .................. 11 Installin !atteries in the 650 (ispla) *nit...................................................................11 &onnectin the Sonde to the YSI 650 (ispla) *nit.....................................................11 Initial Setup........... Setup.................... ................. ................. .................. .................. .................. .................. .......................... .........................................1+ ........................1+ &ommunication &ommunication bet,een the 650 displa) unit and the sonde.................................15 'ain 'enu................................................................................................................16 S)stem setup........................................................................................................16 (ate and ime...................................................................................................16 !arometer &alibration.......................................................................................1" he Po,er Sonde #ption..................................................................................1 Sonde menu..........................................................................................................1$
&alibratin Sonde Sensors..................... Sensors........................................ ...................................... ................................. ..............1$ 1$ &alibratin a clean or ne, sonde................................................................................./0 Supplies reuired....................................................................................................../0 &alibration Instructions............................................................................................./0 1. (issoled #2)en............................................................................................./0 /. (epth.......... (epth................... .................. ................. ................. .................. .................. .................. .......................... ......................................../1 ......................./1 +. &onductiit) and emperature........................................................................../1 4. p3....................................................................................................................../+ 5. #2idation Reduction Potential #RP.............................................................../4 6. &hloroph)ll......................................................................................................../5 ". urbidit)............................................................................................................/6 &alibration Sheet....................................................................................................../ oin data 7 settin up the calibrated sonde to be deplo)ed................................../$
89ter a Sonde (eplo)ment 7 Retriein the sonde: do,nloadin the data and chec;in 9or dri9t.................. d ri9t..................................... ...................................... ...................................... .......................... .......+0 +0 Sonde retrieal.............................................................................................................+0 (o,nloadin Water
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Instructions Instructions - chec;in chec;in 9or dri9t.............. dri9t....................... .................. .................. ................. ........................................++ ................................++ 1. urbidit) and (#...............................................................................................++ /. &onductiit) and emperature..........................................................................+4 +. p3......................................................................................................................+4 4. #2idation Reduction Potential..........................................................................+4 5. (epth.......... (epth................... .................. ................. ................. .................. .................. .................. .......................... ........................................+4 .......................+4 &leanin the Sonde......................................................................................................+5 Re-&alibration Re-&alibration and Re-deplo)ment..... Re-deplo)ment.............. ................. ................. .................. ..............................................+5 .....................................+5 Sensor Storae............................................................................................................+5
Re9erences.................................................................................................+5
Please see the video associated with this manual for visual demonstrations of the procedures presented in this manual. The video times times associated with the different manual sections are as follows:
0=0+ 0=0+ 0=/4 /=+ /=+ 5=+4 6=50 6=50 =1+ =1+ 10=0" 10=0" 1+=+0 14=5/ 1"=/+ //=+4 //=+4 /4=16 ++=+/ ++=+/ 44=54 44=54 4"=51 4"=51 4$=4" 4$=4"
8n Int Introd roduct uction ion to the the YSI YSI Sonde Sonde p.4 p.4 Installi Installin n batte batteries ries in the the 650 650 (isp (ispla) la) *nit *nit p.1 p.11 1 Insta Installi llin n batt batteri eries es in in the Sond Sonde e p.6 p.6 Installi Installin n sensors sensors and remoin remoin plus plus p." p." he he !ul !ul;h ;hea ead d p p. . Insta Installi llin n #ptic #ptical al Sen Senso sors rs p.$ p.$ Installi Installin n Reular Reular Sensors Sensors p.$ p.$ Installin Probe %uard %uard and and &alibration &alibration &up p.10 &onnectin &onnectin the Sonde Sonde to the 650 650 '(S (ispla) (ispla) *nit p.1 p.11 1 &alibratin &alibratin (issoled (issoled #2)en p./0 &alibrat &alibratin in (epth (epth p./1 p./1 &alibratin &alibratin &onductiit) &onductiit) and emperature emperature p./1 p./1 &alibrat &alibratin in p3 p./ p./+ + &alibrat &alibratin in #RP #RP p./4 p./4 &alibrat &alibratin in &hloroph &hloroph)ll )ll p./5 p./5 &alibrat &alibratin in urbi urbidit) dit) p./6 p./6
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YSI 6600 Sonde Introduction !hat is a Sonde" 8 sonde is a piece o9 euipment used to monitor ,ater ualit). It is basicall) a P>& pipe ,ith a circuit board inside see picture to the riht. Properl) calibrated sensors that measure di99erent ,ater ualit) parameters e..: p3: temperature: dissoled o2)en are attached to one end o9 the sonde. he sonde is placed in a la;e or rier and it collects data 9rom the ,ater at reular interals. When the sonde is remoed 9rom the ,ater: the data are do,nloaded to a computer. 89ter routine maintenance and re-calibration: the sonde can then be re-deplo)ed to collect more data. 8 sonde is di99erent than a hand-held meter. 3and-held meters are portable and are used to collect one or a 9e, measurement at a site. Sondes are deplo)ed at a location 9or a lon period o9 time and collect man) measurements. here are some adantaes to usin a Sonde oer traditional ,ater ualit) samplin methods: such as manuall) collectin samples and sendin them to a lab. or e2ample: the Sonde can collect a lot o9 data more 9reuentl): ,ith less e99ort and at a lo,er cost than traditional samplin. #9 course: there are disadantaes to usin a Sonde as ,ell. he Sonde can onl) measure a 9e, ;e) parameters: ,hereas a ,ater sample that is collected and sent to a lab can be anal)@ed 9or a much broader suite o9 parameters. Manufacturer name and model num#er of this Sonde: YSI 6600
!hat parameters does the sonde measure" his particular sonde measures the 9ollo,in parameters in ,ater= 1 / + 4 5 6 "
emperature in derees &elcius &onductiit) p3 #2idationAReduction Potential #RP (issoled #2)en urbidit) &hloroph)ll
or eneral bac;round in9ormation on these parameters and ,h) it is important 9or us to measure them: please see the attached Water
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Important Points to $emem#er 1 The electronics inside the Sonde are easil% dama&ed #% water''' It is er) important that ,ater and other solutions are BC>CR allo,ed to enter the P>& bod) o9 the sonde. Cnsure that all sensors: plus and the cap that coers the cable port are properl) installed at all times. / It is er) important to maintain the rubber #-rins on the sensors and caps. Wheneer a sensor: plu or cap is remoed 9or cleanin or sericin: dirt on these #-rins should be cleaned a,a) entl). !e9ore sensors: plus: or caps are replaced: put a little dab o9 Dr)to2 included ,ith the Sonde on the #-rins Eust use enouh so that it appears shin%( )*T &oop%. #-rins should be replaced eer) one to t,o )ears to ensure the) ;eep a ood ,atertiht seal. + 'a;e sure the batteries inside the sonde hae su99icient chare be9ore deplo)ment see discussion o9 calculated sonde batter) li9e on pae /" o9 this manual. he sonde cannot collect data ,ithout batter) po,er. 4 he end o9 the optical dissoled o2)en sensor must al,a)s be stored in a moist enironment ,hen it is not in use. I9 it is detached 9rom the sonde: it should be stored ,ith a moist spone inside its 9le2ible plastic blac; cap.
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he
p3A#RP probe has a limited li9e. o ma;e the probe last as as possible: the end o9 the p3A#RP probe ,hen attached the sonde should be stored lon-term in p3 electrode storae solution Potassium &hloride +.5 ' or in p3 4 bu99er solution. I9 the probe is not attached to the sonde durin storae: it should be stored in the electrode storae solution 9rom the manu9acturer.
lon to
6 he end o9 the p3A#RP probe is e2tremel) 9raile. It is made up o9 t,o lass bulbs that are easil) bro;en. Beer rest the sonde on the end ,ith the sensors. 8l,a)s ta;e care ,hen usin the calibration cup and probe uard so that )ou donFt ;noc; them aainst the ends o9 the sensors.
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Initial Setup Installin& +atteries in the Sonde !atteries proide the po,er 9or the sonde to collect and store data ,hen it is deplo)ed in the ,ater. 'a;e sure that the sonde contains 9resh batteries be9ore it is deplo)ed or no data ,ill be collectedG This sonde ta,es - t%pe al,aline #atteries. arr% spare #atteries. Step 1 *sin the he2 tool: loosen the batter) lid scre,s. Step / Remoe the lid and install the batteries as sho,n to the le9t. Cnsure that the) hae the proper orientation. Step + !e9ore replacin the lid: it is important to ensure that the orane #-rin is seated completel) ,ithin the rooe on the underside o9 the lid. Press around all its edes to ma;e sure it is not poppin out o9 the rooe an),here alon its lenth. Small retainin tabs alon the #-rin help ;eep it inside the rooe. here is a detailed description o9 ho, to properl) seat this orane #-rin on paes /-1$ to /-/0 o9 the YSI instruction manual. /o not lu#ricate the oran&e *rin&( #ut ma,e sure it is clean. Step 4 'a;e sure that the blac; #-rins that encircle the lid are completel) clean. Bo dirt should be present on or under an) o9 these #-rins. 8ppl) a small amount o9 Dr)to2 to the blac; #-rins so that the) are shin).
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Step 5 Put the lid bac; on the batter) compartment in the orientation sho,ed to the le9t and tihten the scre,s until snu. (o not oertihten the scre,sG I9 the batteries hae been properl) installed: the oltae should read 1/.0 olts or hiher in the sonde Status 'enu. Remoe batteries 9rom the sonde ,hen not in use i.e.: 9or lon-term storae. he batter) compartment o9 this sonde has a lid ,ith a round pressure-release sa9et) ale. In the hihl) unli;el) eent that the batteries should mal9unction and de-as: this ale ,ill release an) pressure buildin up in the batter) compartment. I9 the ale eer opens: the batter) compartment lid must be replaced ,ith a ne, one 9rom the manu9acturer be9ore the sonde can be used aain.
Installin& Sensors o prepare the sonde 9or calibration and data collection: )ou need to install probes also called sensors into the ports on the sonde bul;head. When initiall) receied 9rom the manu9acturer: there are scre,-in plus in the ports to protect the port connections 9rom damae. /o not discard these plu&s' Save all plu&s for future use. Step 1: $emove plu&s from ports
Plus are remoed b) insertin the lon end o9 the installation tool into the holes in the plu tops and entl) unscre,in. he installation tool can be 9ound in the maintenance ;it YSI 65"0. **Always remove plugs and sensors from the sonde with that end of the sonde pointed towards the ground or floor (i.e., oriented as in the picture below).** I9 there is ,ater present on the sensors or plus: remoin them as the) are pointed do,n ,ill allo, ,ater to drain a,a) and preent an) 9lo, o9 ,ater up into the electrical connections inside the ports.
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Step : Install Sensors
his sonde has t,o di99erent t)pes o9 ports capable o9 acceptin sensors= #ptical ports here are 9our optical ports on this sonde labelled as #: !: & and . * port is 9or the optical (issoled *2)en sensor model YSI 6150 port is 9or the optical hloroph)ll sensor model YSI 61+6 T port is 9or the optical Turbidit) sensor model YSI 60/5 + port currentl) has no optical sensor: so it is +lan; 9or no, Reular ports here are t,o reular ports. he) are not labeled: but the) are di99erent= 1 he 6-pin port is 9or the emperatureA&onductiit) sensor model YSI 6560 / he 4-pin port is 9or the p3A#2idation Reduction Potential sensor model YSI 656$
!e9ore insertin an) sensor: ensure that the port is 9ree o9 moistureG *se a clean lint-9ree cloth to dr) i9 necessar). Cnsure that the #-rin on the sensor )ou are insertin is completel) clean o9 dirt. (irt ,ill allo, a path 9or ,ater to enter the electronics o9 the Sonde ,hen it is deplo)ed. 8ppl) a er) liht sheen o9 Dr)to2 to the clean #-rin riht be9ore the sensor is installed to ;eep it in ood shape. *ptical Sensors= Install the optical sensors #efore )ou install the reular sensors. Step 1 Insert the sensor into the appropriate port and entl) rotate until the pins o9 the connectors are alined. Step / Slo,l) 9iner-tihten the nut do,n to,ard the bul;head. **During this step, be careful not to cross thread the nuts! If you meet resistance while fingertightening, "#$, bac% out and begin the process again!** Step + inish tihtenin usin the short end o9 the installation tool so that the seal is ,aterproo9. /o not overti&hten. When tihtened properl): it is normal 9or there to still be a 1-/ mm ap bet,een the nut o9 the optical sensor and the bod) o9 the bul;head.
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$e&ular Sensors= Install a9ter the optical sensors.
Step 1 Insert the sensor into the appropriate port and entl) rotate until the pins o9 the connectors are alined. Step / Push the sensor in,ard so that it seats in the port snul). Slo,l) 9inertihten the nut do,n to,ard the bul;head. **During this step, be careful not to cross thread the nuts! If you meet resistance while fingertightening, "#$, bac% out and begin the process again!** Step + *se the lon end o9 the installation tool to tihten the nut an additional uarter turn until snu. /o not overti&hten. When tihtened properl): there should be no space bet,een the nut o9 the reular sensor and the bod) o9 the bul;head.
Installin& Pro#e uard or ali#ration up
he probe uard protects the sensors durin deplo)ment and allo,s ,ater to 9lo, oer the sensors. #nce all the probes are correctl) installed and calibrated: scre, the uard onto the sonde b) alinin the threads and turnin cloc;,ise until the uard is securel) attached.
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he calibration cup is used durin sensor calibration and 9or lon-term storae ,hen the sonde is not in use. It is a solid plastic cup. he calibration cup is installed in e2actl) the same ,a) as the probe uard.
YSI 620 M/S /ispla% 3nit he YSI 650 '(S displa) unit is an instrument that allo,s the user to tell the sonde ,hat parameters to measure and ho, o9ten to ma;e ,ater ualit) measurements ,hen it is deplo)ed in ,ater. he displa) unit is also used durin calibration o9 the sonde.
Installin& +atteries in the 620 /ispla% 3nit
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he YSI 650 displa) unit reuires 4 & t)pe batteries. han&e the #atteries in a dr% environment. arr% spare #atteries. Step 1 oosen the 4 scre,s on the batter) compartment lid usin a scre,drier and remoe the lid completel). HH)otice the four small holes on the lid. !arometric Pressure is read throuh these 9our holes. )ever cover them with tape or stic,ers or push an)thin throuh these holes e..: pins.HH Step / Insert the batteries bet,een the clips: bein sure that the orientation is correct. 8 9oam pad on the bac; o9 the lid ,ill help to hold the batteries in place ,hen the lid is put bac; on. Step + Re-install the batter) lid and tihten the scre,s. (o not oertihten.
onnectin& the Sonde to the YSI 620 /ispla% 3nit 44It is e5tremel% important to ,eep water out of the electrical connections #etween the displa%( field ca#le and sonde.44
ip= connect the instruments in dr) enironments onl) e..: inside )our ehicle at the 9ield site i9 it is rainin: not outdoors o connect the sonde to the YSI 650 displa) unit: )ou ,ill need= 1 the sonde / the 650 '(S displa) unit ,ith batteries installed + the 9ield cable Step 1 remoe the ,aterproo9 cap 9rom the sonde connector. (o not discard this capG You ,ill need this cap 9or later deplo)ment and lon-term storae. Step / connect the 9ield cable to the sonde connector. here is a uide
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inside the connection that ensures that the pins are properl) lined up. Rotate the cable entl) until the uide enaes. Step + ihten the connector b) turnin cloc;,ise. !hen connected properl%( onl% two threads should #e visi#le' Step 4 8ttach the strain relie9 connector to the metal handle and close the connectorFs openin. Step 5 8t its other end: the 9ield cable plus directl) into the 650 displa) module usin an -pin connector. ine up the uides and pins inside the connector. !e care9ul not to cross-thread. %entl) turn the nut cloc;,ise until it clic;s to loc; the connector in place. 8ttach the second strain relie9 connector to the displa) unit.
Initial Setup Your particular communit)Fs sonde and 650 displa) unit hae settins that are identical to those in all the other sondes and displa) units purchased b) CBR 9or the other communities in the BW. I9 all the communities ,or; ,ith the same instruments: settins and operatin instructions: the ,ater ualit) data enerated at the di99erent sites ,ill be consistent and comparable. his ,ill help to ealuate all the ,ater ualit) data once it is collected. It is important 9or )ou to be able to access and understand these settins but under normal operation: the) should not need to be chaned. 3ere is a description o9 ho, to access the settins=
1+
!elo, is a photo o9 the 650 displa) unit ,ith the di99erent buttons identi9ied.
he rrow 7e%s are used to moe up and do,n throuh the di99erent selections in the menus. he 8nter ,e% is used to con9irm a selected menu item. he 8scape 98S ;e) is used to return to the preious screen )ou ,ere at in the menu. he number buttons are used to enter ,ords and data 9or e2ample site names. he 9ollo,in uide indicates the number o9 times )ou need to press each number button to access indiidual capital and lo,er case letters to spell out site names=
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I9 )ou are 9indin that the screen is di99icult to read: )ou can ma;e the screen either dar;er or lihter b) holdin do,n the !ac;liht ;e) and pressin either the up arro, to dar;en or the do,n arro, ;e) to lihten.
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ommunication #etween the 620 displa% unit and the sonde Bote= 'a;e sure onl) t,o threads o9 the communications communications connector are isible once the cable has been attached. I9 more than t,o threads are sho,in a9ter attachin the cable: ,ater WI et into the connection and potentiall) cause harm to the connector. When ph)sicall) connected toether usin the 9ield cable: the 650 displa) unit and sonde can e2ist in t,o di99erent communication states. 1 In the first state : the displa) unit is connected electronicall) to the sonde and basicall) tal;in toJ it. In this state: the small picture in the bottom riht hand corner o9 the 650 displa) is a picture o9 the sonde. In this communication state, you can calibrate the sonde and change sonde settings.
/ In the second state: the displa) unit is not connected electronicall) to the sonde and is not tal;in to itJ. In this case: the icon in the bottom riht hand corner o9 the 650 displa) ,ill be the 650 displa) itsel9.
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or the ne2t 9e, sections: ,e are Eust settin up the basic displa) unit itsel9: so ,e do not need to be connected to the sonde. When )ou are ,or;in ,ith the instrument: ma;e sure it is in the communication state )ou desire b) chec;in the icon in the corner.
Main Menu 89ter ph)sicall) connectin the sonde to the displa) unit as described in the preious sections: turn on the YSI 650 displa) unit usin the reen po,er button. Botice that in this picture )ou can see that ,e are not tal;inJ to the sonde. You ,ill see the 9ollo,in Main Menu=
S%stem setup /ate and Time To set or chan&e the date and time in the YSI 620 displa% unit:
Step 1= Select S%stem setup 9rom the main menu
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Step /= Select /ate ; time
Step += Select d
here is an option under S%stem setup called ali#rate +arometer . he barometer in the 650 displa) unit should onl) eer be calibrated usin local barometric pressure i.e.: pressure that has not been corrected 9or sea leel. ocal ,eather serice barometric
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pressure is o9ten corrected to sea leel and is there9ore not the same as the true local barometric pressure alue reuired b) this instrument. Be, instruments are prorammed to measure true local pressure and should remain stable 9or a lon time be9ore reuirin re-calibration. I9 )ou do need to calibrate the barometer= Step 1 (etermine the local uncorrected barometric pressure. I9 there is no true: local laborator) barometer readin aailable: a ,eather serice readin in mm o9 3 that has been corrected to sea leel can be uncorrectedJ accordin to the 9ollo,in 9ormula= rue !P K Lsea-leel corrected !PM 7 L/.5 2 local altitude in 9t aboe sea leelA100M Step / rom the main menu: select S%stem Setup then ali#rate +arometer Step + Input the local barometric pressure usin the ;e)pad Step 4 Press the enter ;e)
The Power Sonde *ption
*nder Main Menu and S%stem Setup: there is an option called Power sonde that can be clic;ed on chec;mar; or o99 no chec;mar;.
When Po,er sonde is clic;ed #B: the displa) unit po,ers the sonde. When Po,er sonde is clic;ed #: the displa) unit does not po,er the sonde and the sonde relies on its o,n batteries 9or po,er. It is important that the Po,er sonde option be *>> ,hen the sonde is oin to be deplo)ed. #ther,ise: the displa)ed batter) li9e in9ormation i.e.: ho, lon the sonde can be deplo)ed ,ill be incorrect and it is possible that the sonde ,ill not be po,ered and ,ill not store data ,hen deplo)ed . It is #est to ?ust ensure that #oth the displa% unit
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and sonde have &ood #atteries in them at all times and leave the Power sonde option *>> 9no chec,mar, at all times.
Sonde menu Bo, that ,e hae set up the 650 displa) unit: it is time to connect the displa) unit electronicall) to the sonde. rom the 650 Main menu : select Sonde menu. 8 bo2 ,ith the ,ord connectinJ ,ill appear uic;l) and then disappear. he small picture in the bottom riht hand corner o9 the 650 displa) ,ill chane to sho, a picture o9 the sonde. You are now in the menu system for the sonde. In this series o9 menus: )ou can calibrate the sonde and chane sonde settins. You ,ill no, see the 9ollo,in menu= he 9irst screen )ou ,ill see is the Main menu for the sonde . (o not con9use this menu ,ith the main menu 9or the 650 displa) unit. #rient )oursel9 usin the icon in the bottom riht corner i9 necessar). You can e2it the sonde menu s)stem 9rom this screen b) pressin the escape ;e). he sonde ,ill disconnect electronicall) and )ou ,ill be returned to the main menu o9 the 650 displa) unit.
ali#ratin& Sonde Sensors ali#ration is an important process that helps the user ensure that the sonde sensors are measurin properl) and eneratin accurate ,ater ualit) data. We basicall) hae to train the sensors so that the) N;no,F ho, to measure the parameters ,e are
/0
interested in. &alibration reuires solutions called standards ,ith ;no,n leels o9 the chemical parameters ,e ,ill be measurin. We calibrate one sensor at a time. he sonde sensors are immersed in the standards one standard at a time: and the eri9ied concentration o9 the chemical that is present is input into the instrument. his ,a): the instrument can lin; the sinal it is measurin to the proper: ;no,n concentration. hen: ,hen the instrument measures an un;no,n leel o9 the same parameter in the la;e or rier bein studied: it uses the calibration in9ormation to calculate the concentration o9 the chemical in the ,ater. It is critical that the sonde be calibrated be9ore each deplo)ment. &alibrations o9 the di99erent parameters measured b) this sonde can inole usin one sinle standard a one point calibration or / standards a /-point calibration
ali#ratin& a clean or new sonde Supplies re@uired -Standard appro2imate olume reuired -(e-ioni@ed ,ater O 4 -&onductiit) standard 141+ SAcm K1.41+ mSAcm 500 m -p3 " standard 500 m -p3 10 standard 500 m -urbidit) standard 0 B* 500 m -urbidit) standard 1/6 B* 500 m HHBote that this turbidit) standard has t,o alues dependin on the YSI sensor bein used. he BW sondes are usin turbidit) sensor model YSI 61+6. here9ore: the second turbidit) standard alue that )ou ,ill need to input is 1/6 B*HH -#RP standard QobellFs solution 500 m -&alibration ,or;sheet located at the end o9 this section -Sonde maintenance ;it -Protectie loes 7 ,ear these durin all calibration steps
ali#ration Instructions
1. /issolved *5%&en HHBote= !e9ore dissoled o2)en calibration: ma;e sure */* sat A B and */* m&
/1
sure there are no ,ater droplets on the dissoled o2)en sensor or on the temperature sensor. Cnsure these t,o sensors are not submered in ,ater. Step + rom the Sonde menu select ali#rate( press enter. Step 4 Select *ptic* /issolved *5% : press enter. Step 5 Select */*sat A: press enter. Step 6 Select 1 point : press enter. Step " 8nter the #arometric pressure readin& at the bottom riht corner o9 the screen and press enter. $ecord the #arometric pressure readin& on the calibration sheet. Step 89ter allo,in the dissoled o2)en readin to stabili@e 9or 5-15 minutes: record the precali#ration value in m&
. /epth Step 1 'a;e certain the depth sensor is in air and not immersed in an) solution. Step / rom the Sonde menu select ali#rate( press enter. Step + Select Pressure#s: press enter. Step 4 Input 0.00 and press enter. Step 5 Wait +0 seconds until the readins hae stabili@ed. $ecord the precali#ration depth readin& on the calibration sheet.
//
Step 6 Press enter to con9irm the calibration. Step " $ecord the postcali#ration depth readin& on the calibration sheet. Step Press enter to continue and then escape to return to the main menu.
C. onductivit% and Temperature Step 1 Rinse the sensors and calibration cup once ,ith de-ioni@ed ,ater. Step / Rinse the sensors and calibration cup + times ,ith conductiit) solution 141+ SAcm K 1.41+ mSAcm at /5 T&. Step + Scre, the calibration cup onto the end o9 the inerted Sonde i.e.: sensors should be pointin to ceilin. Step 4 ill the calibration cup ,ith conductiit) standard 141+ SAcm K1.41+ mSAcm at /5 T& so that the conductiit) probe and the temperature sensor are completel) submersed into the calibration solution i.e.: past the ent hole. Rotate the sonde to ensure that there are no air bubbles inside an) part o9 the conductiit) sensor. Wait a minute be9ore moin to the ne2t step to allo, the temperature to stabili@e. Step 5 rom the Sonde menu select ali#rate and press enter. Step 6 Select onductivit% and press enter. Step " Select Spond speci9ic conductance and press enter. Step *se the ;e)pad to enter the ;no,n concentration o9 the conductiit) standard in mS
/+
loo; bac; at preious calibration sheets to determine the historical alue. I9 the alue doesnFt meet these criteria: the calibration standard ma) be o99GG r) another bottle o9 calibration standard. I9 the constant is still o99: (# B# (CP#Y this sensor: and contact CBR 9or support. Step 1+ urn the sonde bac; to normal orientation. Remoe the calibration cup and add a thermometer to the solution and allo, the readin to stabili@e. $ecord the temperature value from the thermometer in D in the bo2 9or temperature standardJ on the calibration ,or;sheet. Step 14 Cmpt) the calibration cup. Rinse all the sensors and the calibration cup thorouhl) ,ith de-ioni@ed ,ater be9ore calibratin the ne2t parameter.
=. pF HHBote= p3 reuires a /-point calibrationHH HHBote= !e9ore p3 calibration: ma;e sure p3 in milliolts is turned on under Sonde Menu( $eport.HH Step 1 Rinse the sensors and calibration cup once ,ith de-ioni@ed ,ater. Step / Rinse the sensors and calibration cup ,ith p3 " solution: repeat this step t,o more times 9or a total o9 + rinses ,ith p3 " solution. Step + With the sonde inerted: 9ill the calibration cup ,ith p3 " solution to immerse the p3 bulb and the temperature sensor on the conductiit) probe. Step 4 rom the Sonde menu select ali#rate( press enter. Step 5 Select IS81 pF: press enter. Step 6 Select Point: press enter. Step " Input the alue o9 the p3 " solution at your calibration temperature 9rom the table on the standard bottle at the prompt and press enter. or e2ample: i9 )ou are calibratin at /0 T&: the input alue ,ould be ".0/. Press enter. he alues o9 all the sensors ,ill appear on the screen and chane as the) stablili@e. Step $ecord the precali#ration readin& in pF units on the calibration sheet. Step $ When the p3 readin does not chane sini9icantl) 9or +0 seconds: press enter. he calibration ,ill be accepted.
/4
Step 10 $ecord postcali#ration readin& in pF units and the postcali#ration pF millivolts on the cali#ration sheet . he p3 milliolts alue should be bet,een -50 m> and U50 m>. Press enter to continue. Step 11 Return the sonde to normal orientation. Cmpt) the calibration cup. Rinse the sensors and calibration cup once ,ith de-ioni@ed ,ater. Step 1/ Rinse the sensors and calibration cup three times ,ith p3 10 solution. Step 1+ With the sonde inerted: 9ill the calibration cup ,ith p3 10 solution to immerse the p3 bulb and the temperature sensor on the conductiit) probe. Step 14 Input the alue o9 the p3 10 solution at your calibration temperature 9rom the table on the standard bottle at the prompt and press enter. or e2ample: i9 )ou are calibratin at /0 T&: the input alue ,ould be 10.06. Press enter. he alues o9 all the sensors ,ill appear on the screen and chane as the) stablili@e. Step 15 $ecord the precali#ration readin& in pF units Step 16 When the p3 readin does not chane sini9icantl) 9or +0 seconds: press enter. he calibration ,ill be accepted. Step 1" $ecord postcali#ration readin& in pF units and the postcali#ration pF millivolts on the cali#ration sheet . he p3 milliolts alue should be bet,een 165 m> and 10 m> below the pH 7 mV reading . I9 the di99erence is less than 165 m> or more than 10 m>: the re9erence solution inside the probe ma) be too old. (# B# (CP#Y this p3 probe. he li9espan o9 re9erence solution inside the p3 sensor is onl) 1.5-/ )earsG Press enter to continue and escape to return to the main menu. Step 1 Return the sonde to normal orientation. Cmpt) the calibration cup and rinse the sensors and calibration cup ,ith de-ioni@ed ,ater.
2. *5idation $eduction Potential 9*$P HHBote= &alibrate #RP after calibratin p3HH Step 1 Prepare the Qobell calibration solution accordin to the instructions on the bottle= #pen the bottle and add 1/5 m o9 de-ioni@ed ,ater to the dr) po,der. he solution has a shel9 li9e o9 6 months once mi2ed. Step / Rinse the sensors and calibration cup ,ith de-ioni@ed ,ater and then some o9 the Qobell solution. We hae alread) completed these steps Step + Pour the Qobell solution into the calibration cup and immerse the p3A#RP sensor and the temperature sensor in the solution.
/5
Step 4 rom the Sonde menu select ali#rate( press enter. Step 5 Select IS8 *$P : press enter. Step 6 Cnter the alue o9 the #RP solution in milliolts at the calibration temperature. See the table in the instruction manual. or e2ample: i9 ,e are calibratin at /0 derees &: the alue to enter ,ill be /+".5 m>. Cnter this alue and press enter.
Step " 'onitor the stabili@ation o9 the #RP and temperature readins. #nce the) are stable 9or appro2imatel) +0 seconds: record the precali#ration *$P value in millivolts on the calibration ,or;sheet. I9 the probe is 9unctionin ,ithin speci9ications: the #RP readin should be ,ithin the rane o9 //1-/41 milliolts at normal ambient temperature. Step Press enter to con9irm the calibration and record the postcali#ration *$P value in millivolts on the calibration ,or;sheet. Step $ Press enter to continue and escape to return to the main menu. Step 10 Return the sonde to normal orientation: empt) the calibration cup and rinse the sensors and calibration cup ,ith de-ioni@ed ,ater.
6. hloroph%ll Step 1 Rinse the sensors and calibration cup thorouhl) ,ith deioini@ed ,ater. Submere the chloroph)ll sensor in de-ioni@ed ,ater. Step / rom the Sonde menu select ali#rate( press enter. Step + Select *ptic hloroph%ll : press enter. Step 4 Select hl $>3 Gero : press enter.
/6
Step 5 Input the &hl R* alue o9 0 at the prompt and press enter. Step 6 When the readins hae stabili@ed: record the precali#ration chloroph%ll readin& on the calibration sheet. Press enter to con9irm the calibration and @ero the sensor. Step " $ecord the postcali#ration chloroph%ll readin& on the calibration sheet. Press an) ;e) to return to the &alibrate menu. Press enter to continue. Step %rab samples must be collected near the sonde at certain times durin deplo)ment and the &hloroph)ll content determined in a lab. Bote the e2act dates and times that these samples are collected. hese samples ,ill allo, the relatie readins collected b) the sonde to be e2pressed as real &hloroph)ll concentrations.
H. Tur#idit% HHBote= turbidit) reuires at /-point calibrationHH HHBote= turbidit) must be calibrated ,ith the sonde in the upriht position.HH Step 1 Cnsure that the sensors and surroundins hae been cleaned and are 9ree o9 debris 9rom past deplo)ments. 8n) solid particles ,ill contaminate the standards durin the calibration and cause the instrument to collect incorrect data. Step / Pour enouh o9 the 0 B* standard slo,l) into the calibration cup aoidin bubbles so that the end o9 the turbidit) sensor is submered a 9e, mm. Cnae onl) one thread ,hen scre,in the calibration cup onto the sonde to ;eep the sensor 9ace as 9ar a,a) as possible 9rom the calibration cup bottom. he calibration cup itsel9 is a source o9 inter9erence. Step + Reduce the lihtin in room and ,rap a blac; ba around the cup. Step 4 rom the Sonde menu select ali#rate( press enter. Step 5 Select *ptic T Tur#idit%61C6 : press enter Step 6 Select Point: press enter Step " Input the alue 0 B* at the prompt: press enter. he screen ,ill displa) readins. Step 8ctiate the sensor ,iper 1-/ times b) pressin Clean *ptics . Step $ When the readins hae stabili@ed: $ecord the precali#ration tur#idit% readin& for 0 )T3 on the calibration ,or;sheet.
/"
Step 10 Press enter to con9irm the 9irst calibration and record the postcali#ration value for 0 )T3 on the calibration ,or;sheet. Step 11 Press enter to continue. Step 1/ Cmpt) the cup and rinse the cup and sensors ,ith (I ,ater. (r) both the calibration cup and the turbidit) sensor. &ompressed air is hand) 9or this step. Step 1+ Pour enouh o9 the 1/6 B* standard slo,l) into the calibration cup aoidin bubbles so that the end o9 the turbidit) sensor is submered a 9e, mm. Cnae onl) one thread ,hen scre,in the calibration cup onto the sonde to ;eep the sensor 9ace as 9ar a,a) as possible 9rom the calibration cup bottom. he calibration cup itsel9 is a source o9 inter9erence. **Note that this turbidity standard has two values depending on the YSI sensor being used. he N! sondes use turbidity sensor model YSI "#$". herefore% the second turbidity standard value that you will need to input is #&" N'** Step 14 Reduce lihtin in room and ,rap blac; ba around calibration cup or Ear. Step 15 Input the alue 1/6 B* at the prompt: press enter. he screen ,ill displa) readins. Step 16 8ctiate the sensor ,iper 1-/ times b) pressin Clean *ptics. Step 1" When the readins hae stabili@ed: record the precali#ration tur#idit% readin& for 16 )T3 on the calibration ,or;sheet. Step 1 Press enter to con9irm the second calibration and record the postcali#ration value for 16 )T3 on the calibration ,or;sheet. Step 1$ Cmpt) the cup and rinse ,ith (I ,ater.
/
ali#ration Sheet (ateAime &hec;ed b) ime since last calibration
Sonde ocation
Parameter !arometric Pressure mm3 emperature T& sonde emperature T& thermometer readin Speci9ic &onductance VSAcm (issoled #2)en p3 urbidit) B* #2idation Reduction Potential #RP &hloroph)ll (epth m
Standard 3sed sonde
fter /eplo%ment $eadin& 9if applica#le
thermometer 0 141+ local mA 4 " 10 0 1/+ Qobells (I ,ater 0 - in air 1 m - in ,ater
+efore ali#ration $eadin&
e d n o S n a e l
.
fter ali#ration $eadin&
e t a r # i l a .
&omments YSI /8TIBS 44V8$Y IMP*$T)T T* >IBB TFIS F$T I)''44 Parameter value accepta#le ran&e 5.0 0.45 X not more than 0.1 9rom historical record conductiit) cell constant should be bet,een 0."5 - 1./5 #(# ain alue -14." 6 non-ented pressure o99set 0 '> 50 p3 m> bu99er " -1"" 9rom p3 " m> p3 m> bu99er 10 165 to 10 m> span bA, p34-" m> -165 to -10 m> span bA, p3"-10 m> Maintenance $ecord Sensor !atteries chaned
ompleted 9Y( )( )<
Tas, I9 not: batter) oltae=
/$
Bo&&in& data settin& up the cali#rated sonde to #e deplo%ed his instrument can lo store data in t,o locations= 1 YSI 650 displa) unit / he sonde #ption / loin to the sonde is the option used 9or lon-term deplo)ments i.e.: the sonde is not attached to the displa) unit and is put into a rier or la;e to collect seeral ,ee;s ,orth o9 data. (or these N! )N sondes% we will be logging data continuously at a set time interval +every #, minutes- to the memory within the sonde. To set up lo&&in& and deplo% the sonde:
Step 1 'a;e sure that the sonde is not bein po,ered b) the 650 620 main menu : s%stem setup: the bo2 9or power sonde from 620 should B# be chec;ed. he
Bo&&in& data settin& up the cali#rated sonde to #e deplo%ed his instrument can lo store data in t,o locations= 1 YSI 650 displa) unit / he sonde #ption / loin to the sonde is the option used 9or lon-term deplo)ments i.e.: the sonde is not attached to the displa) unit and is put into a rier or la;e to collect seeral ,ee;s ,orth o9 data. (or these N! )N sondes% we will be logging data continuously at a set time interval +every #, minutes- to the memory within the sonde. To set up lo&&in& and deplo% the sonde:
Step 1 'a;e sure that the sonde is not bein po,ered b) the 650 620 main menu : s%stem setup: the bo2 9or power sonde from 620 should B# be chec;ed. he sonde must be po,ered b) its o,n batteries. Step / rom the sonde Main menu: select $un Step + Select 3nattended Step 4 #n the *nattended setup menu: the settins should read as 9ollo,s= Interval K 00=15=00 Start date K enter the date o9 deplo)ment in ddAmmA)) Start time K enter the appro2imate time o9 deplo)ment as the start time /uration da%s K number o9 da)s o9 deplo)ment note= must be less than batter) li9e: belo,G >ile K Cnter a name 9or the 9ile here. Site K Cnter the site name here +at volts K !atter) oltae ,ill sho, here e..: 1/." >olts +at life K +atter% Bife 9da%s. Ta,e note of this value'' his is the calculated batter) li9e da)s based on the batter) oltae and the number o9 measurements that ,ill be ta;en ien the 15 minute samplin interal. With ne, batteries: the prorammin ,ill displa) a bat li9e o9 appro2imatel) 5 da)s. his is onl) an estimate and assumes a temperature o9 /0T&. he actual batter) li9e ma) be seeral ,ee;s less than this estimate: so ;eep this in mind ,hen decidin ,hen to retriee and do,nload the sonde. When the batteries run out o9 po,er: the sonde ,ill stop loin data. Proceed to step 6 onl% if %our sonde has alread% #een cali#rated. If not( cali#rate the sonde prior to deplo%ment' Step 5 Select Start oin and press enter to bein data collection. Step 6 (isconnect the 650 and ensure that the cap is coerin the electronics port ,here the 9ield cable ,as remoed. 'a;e sure onl) t,o threads o9 the communications communications connector are isible once the re) cap has been installed correctl). I9 +0
more than t,o threads are sho,in a9ter installin the cap: ,ater WI et into the connection and potentiall) cause harm to the connector. Step " (eplo) the sonde in the rier or la;e bein studied. Ma,e sure that it is well attached to a raft( a #uo%( shore( or prefera#l% a com#ination G
fter a Sonde /eplo%ment $etrievin& the sonde( downloadin& the data and chec,in& for drift Sonde retrieval *pon arriin at the sonde: ta;e note o9 site conditions such as temperature: precipitation in last /4 hours: 9lo,: color o9 ,ater: unusual odors: siltation: material in bottom o9 rier: and an) other obseration )ou deem important. Record all this in9ormation in )our 9ield boo;. Remoe the sonde 9rom the ,ater bod). I9 the sonde is still loin data: hoo; up the 9ield cable and 650 displa) unit and connect to the Sonde Menu. Select $un: 3nattended Sample : and then Stop Bo&&in&. Return to the lab.
/ownloadin& !ater Jualit% /ata from the Sonde to a omputer Step 1 Install the CcoWatch so9t,are on )our computer. (o this b) placin the &( in )our computerFs &( drie. 8n InstallShield Wi@ard ,ill automaticall) start on most computers. ollo, the prompts to complete the installation. Step / &lear an) debris 9rom the bul;head connector cap. I9 there is ,ater present: dr) the electrical connections thorouhl) be9ore attemptin an) connectionsGG Remoe the cap and connect the calibration cable YSI 606" pictured belo, to the sonde and to )our computerFs com port. 3TI*): he 606" calibration cable is 9or laborator) use onl) - it is not ,aterproo9 and should neer be submersedG
+1
Step + Select the CcoWatch icon on )our computer des;top and open the so9t,are. Step 4 &lic; &omm on the toolbar Step 5 &lic; Sonde on the drop do,n menu that appears Step 6 Select the appropriate com port that the 606" calibration cable it plued into and clic; #D. Step 5 8 terminal inter9ace ,indo, li;e that sho,n belo, ,ill appear. I9 onl) a Z sho,s up in the ,indo,: t)pe menu and press enter. he screen should no, appear as sho,n belo,. his is the main menu screen.
Step 5 )pe C-ile to ie, data handlin options.
+/
8ll data 9iles stored in the sonde hae a .dat e2tension. he 9ile ,ith the .lp e2tension contains the calibration record o9 the sonde. Step " )pe C-
fter deplo%ment chec, for drift 89ter a deplo)ment: it is important to see i9 the accumulation o9 alae and dirt on the sonde 9oulin has caused the calibration o9 the sonde to chane this is called drift. (ri9t a99ects the data collected durin the deplo)ment and must be accounted 9or. his chec; 9or dri9t ,ill be done b) measurin standards as soon as the sonde has been ta;en out o9 the rier or la;e and returned to the lab. It is important not to clean the ++
sonde until these chec,s have #een completed' It is also important not to rinse the sensors too much durin these steps to et an accurate measurement o9 the 9oulin e99ect.
Supplies needed to chec, for drift: Standard olume reuired -(e-ioni@ed ,ater 4 -urbidit) standard 0 B* 500 m -p3 " 500 m -p3 10 500 m -&onductiit) standard 141+ SAcm 500 m -QobellFs solution 500 m -&alibration sheet -Sonde maintenance ;it -Protectie loes 7 ,ear these durin all steps
Instructions chec,in& for drift 1. Tur#idit% and /* Step 1 Put de-ioni@ed ,ater 0 B* into the calibration cup so that onl) a 9e, mm o9 the turbidit) sensor are in contact ,ith the ,ater ,ith sonde riht-side-up. Step / Scre, the YSI onto the calibration cup onl) enain a 9e, threads so air can di99use into calibration cup. Step + 8ctiate the turbidit) ,iper: reduce lihtin in room and ,rap blac; arbae ba oer calibration cup to reduce lihtin. Step 4 $ecord the tur#idit% readin& on the calibration sheet under a9ter deplo)ment readinJ. Step 5 Remoe the ba. Pour out the 0 B* standard until there are Eust a 9e, m le9t in the calibration cup. Cnsure that the (# sensor and temperature sensor do not hae ,ater droplets on them. he) should both be dr) and neither should be submered in the standard. Re-attach the calibration cup: enain onl) a 9e, threads so that air can di99use into the cup. Wait 5-10 minutes until the air in the cup saturates ,ith ,ater. 89ter allo,in the dissoled o2)en readin to stabili@e: record the value in m&
+4
. onductivit% and Temperature Step 1 Scre, the calibration cup onto the YSI and inert it sensors pointin to ceilin. Step / ill the calibration cup ,ith speci9ic conductiit) standard 141+ SAcm so that the conductiit) sensor and the temperature sensor are submersed into the calibration solution. Step + $ecord the value of conductivit% in ES
C. pF Step 1 Rinse the p3 sensor onl) once ,ith small amount o9 p3 " solution. Step / With the sonde inerted: 9ill the calibration cup ,ith p3 " solution to immerse the p3 sensor and the temperature sensor on the conductiit) probe. Step + $ecord the pF readin& on the calibration sheet under a9ter deplo)ment readinJ . Step 4 Rinse the sensors and cup ,ith a small amount o9 ,ater.
=. *5idation $eduction Potential Step 1 Rinse the p3A#RP sensor ,ith a small amount o9 QobellFs solution. Step / ill the calibration cup ,ith QobellFs solution to immerse the p3A#RP sensor and the temperature sensor on the conductiit) probe. $ecord the *$P readin& in millivolts on the calibration sheet under a9ter deplo)ment readinJ .
2. /epth Step 1 $ecord the preclean depth readin& in air on the calibration sheet under a9ter deplo)ment readinJ
+5
44/o not reuse an% of the standards used a#ove to chec, for drift for an% future sonde cali#rations. The% have #een contaminated #% the al&ae( dirt and &rime present on the retrieved sonde'44
leanin& the Sonde 89ter chec;in the a9ter-deplo)ment readins: clean the sonde accordin to the manu9acturerFs recommendation. *se mild dish soap: a so9t toothbrush and the ,ire brush proided in the maintenance ;it. Rinse thorouhl) ,ith clean ,ater a9ter cleanin. 89ter cleanin: ensure all sensors are properl) rinsed ,ith de-ioni@ed ,ater.
$eali#ration and $edeplo%ment &alibrate the 9reshl) cleaned sonde as described aboe in the calibration section. Set up loin and re-deplo) the sonde in the ,ater bod) bein studied.
Sensor Stora&e It is recommended to ;eep the sensors attached to the sonde a9ter installation rather than remoin them unnecessaril) 9or storae. he optical dissoled o2)en sensor should al,a)s be stored ,et. he p3A#RP probe has a limited li9e and should be stored in p3 electrode storae solution Potassium &hloride +.5 ' or in p3 4 bu99er solution. p3 4 bu99er solution is 9ine 9or lon-term storae o9 all the other probes as ,ell.
Jualit% ontrol &ollect triplicate rab samples 9or lab anal)sis at the beinnin: middle and end o9 eer) 9ield season. he lab anal)ses ,ill proide a comparison 9or the data bein collected b) the sonde. Cer) time a sonde is collected: do,nloaded: calibrated and re-deplo)ed: ta;e one ,ater sample 9or lab anal)sis.
$eferences hese instructions hae been assembled and reproduced ,ith modi9ications 9rom= YSI incorporated 6-Series 'utiparameter Water
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