Biochem Ab

ORGANIC CHEMISTRY and BIOCHEMICAL ENGINEERING Refresher Course

Physical and Chemical Principles Quice Review Room 608-610, Doña Amparo Building, G. Tolentino Street, Sampaloc Manila

Biochemical Engineering 59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 69. 70. 71. 72. 73. 74. 75. 76. 77. 78. 79. 80. 81. 82. 83. 84. 85. 86. 87. 88.

Fats All of these Decrease Saponification Micelles Hardening Rancidity Steroids Terpenes Carbohydrates (+)-Glucose (–)-Fructose 16 Uronic acid (+)-Mannonic acid Kiliani-Fischer synthesis Ruff degradation Both A and B (+)-Sucrose D-(+)-glucose and D-(–)-fructose Starch Glycogen Proteins Glu-Cys-Gly Sickle-cell anemia Nucleic acids Nucleotide 65.0 mM/s 0.100 mM 46.43 mM

Solution to Linked Problem 86-88:

86.

Eadie-Hofstee plot:

 =   + ax [S]   [S] (mM) 0.02 0.04 0.07 0.10 0.15 0.20 0.30 0.50 0.70

V  (mM/s)

V   /[S] (s–1)

10.83 18.57 26.76 32.50 39.00 43.33 48.75 54.17 56.88

541.5 464.25 382.2857 325 260 216.65 162.5 108.34 81.2571

Linear regression data (V   /[S] vs. V ): Slope = –1/K M   = –9.996 mM–1 y -Intercept = V max /K M = 649.806 s–1

 = 649.806 s− = 649.806 s−(9.9961mM−)=65.0 mM/s 87.

Hanes-Woolf plot:

[S] = [S] +   ax ax [S] (mM) 0.02 0.04 0.07 0.10 0.15 0.20 0.30 0.50 0.70

V  (mM/s)

[S]/V  (s) 1.8467×10–3 2.1540×10–3 2.6158×10–3 3.0769×10–3 3.8462×10–3 4.6157×10–3 6.1538×10–3 9.2302×10–3 1.2307×10–2

10.83 18.57 26.76 32.50 39.00 43.33 48.75 54.17 56.88

Linear regression data ([S]/V  vs. [S]): Slope = 1/V max = 0.0154 s/mM y -Intercept = K M /V max = 1.5389×10–3 s

 = 1.5389×10− sax = 1.5389×10− s(0.01541s/mM) = 0.1 00 mM 88.

Lineweaver-Burke plot:

[S] (mM) 0.02 0.04 0.07 0.10 0.15 0.20 0.30 0.50 0.70

1 =  1 + 1  ax [S] ax V   (mM/s)

10.83 18.57 26.76 32.50 39.00 43.33 48.75 54.17 56.88

1/[S] (mM–1) 50 25 14.2857 10 6.6667 5 3.3333 2 1.4286

1/V (s/mM) 0.0923 0.0539 0.0374 0.0308 0.0256 0.0231 0.0205 0.0185 0.0176

Linear regression data (1/V vs. 1/[S]): Slope = K M   /V max = 1.5384×10–3 s y -Intercept = 1/ V max = 0.0154 s/mM

1 = 1.5384×10− s 1 +0.0154 s/mM= 1.5384×10− s( 1 ) + 0.0154 s/mM = 0.02155 s/mM [S]  0.25 mM  = 0.021551 s/mM = 46.4 mM/s