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Scope and Limitations
This study aims to determine the colonization of clinically relevant pathogens on nursing Caps of nurses assigned at the wards of Southern Philippines Medical Center (SPMC); Medicine ward, Pay ward 1, and obstetric ward. The criteria used for the selection of the subjects were as follow: (a) nursing students of Ateneo de Davao University (AdDU) and staff nurses of SPMC; (b) female; (c) exposed to the wards of Southern Philippines Medical Center (SPMC); Medicine ward, Pay ward 1, and obstetric o bstetric ward. The researchers used one (1) culture medium namely the Mueller Hinton medium for culturing the bacteria. The experiment took place at the AdDU biology laboratory. Local transport vehicles available within the city and private vehicles were used for the transportation of the researchers in completing the study. The group provided eighteen (18) pieces of nursing caps to the student nurses, nine (9) of which were washed daily and the other nine (9) caps were unwashed and were repeatedly exposed in the same wards. Additional six (6) nursing caps were bought daily for the controlled group of caps. The controlled group of caps was directly brought to the biology laboratory for swabbing. Swabbing was done daily using the streak plate method. The number of subjects were set to six (6) per ward in order to have an even number of subject for the sake of the statistical treatment, therefore enabled us to have a statistically valid valid rese resear arch ch study study.. The The temp temper erat atur uree was was meas measur ured ed usin using g notin noting g the the rang rangee of temperature of every room or ward from 25ºC-45ºC with the intention to coincide with the ability of other pathogens to grow in such condition. The wards were also ventilated. It took the researchers two (2) months to complete this study, four (4) days of which was spent for the gathering of data and culturing the bacteria. The researchers limited the incubation period within 24-hours giving the bacteria enough time to thrive. Even Even thou though gh the the caps caps were were expos exposed ed in the the diff differ erent ent ward wardss of SPMC SPMC,, the the researchers focused the study only on the extent of colonization of clinically relevant pathogens on the caps regardless of the wards they were exposed in. The researchers only choose to use different wards in order for the researchers to have a greater number of
subjects for the research study that was useful in providing a more reliable research study.