Report 2

VIETNAM NATIONAL UNIVERSITY – HCMC INTERNATIONAL UNIVERSITY UNIVERSIT Y SCHOOL OF BIO-TECHNOLOGY

REPORT 2 EXPERIMENT 1: STAINING, BACTERIAL COLONY AND AND CELL CE LL MORPHOLOGY

Subj!": Cell Culturing Lab. D#" $#b %&'(&)*: 17/11/2016. S"u*+" N#):

Đỗ Trương Anh Thư S"u*+" ID: BTBCIU106 L#b %#&"+&:

 !gu"#n Th$" Th$" %ung Thi Tr&nh !g'( Anh Thư I+"&u!"(&: )*(. Tran Le +,ng !g,(

I. 

ABSTRACT

*tain- are -,luti,n- that (,ntain a -,lute (alle a (hr,,h,re i--,le in a -,lent. A (hr,,h,re i- the (,l,r ,--e--ing ,rti,n , the -,luti,n an ithere,re re-,n-ible ,r the -tain- (,l,r. 3hen the -tain i- alie4 there i- an attra( attra(ti, ti,nn bet5ee bet5eenn the the negati negatiel el"" (harg (harge e (ell (ell -ura( -ura(ee an the ,-iti ,-itiel el"" (harge (hr,,h,re4 leaing t, the -ura(e , the (ell taing ,n the (,l,r ,  the -tain. In thi- eerient4 5e 5ill reare a -ear an -tain three -e(ie- ,   ba(teria4 5hi(h i- 89 C,li4 *tah"l,(,((u- Aureu-4 an *al,nella b" ,ing gra -tain rea(ti,n an u-ing ;+ a- an ini(at,r ,
INTRODUCTION

The ing agent4 al(,h,l4 i- ae net. Thi- i- the (ru(ial4 ierentiating -te. The eh"rating ee(t , the al(,h,l (au-e- the eti,gl"(an9ri(h (ell 5all , the ati,n , the
9 9 9 9 9 9 9 9 9 9

*tah tah""l,(, l,(,(((u(u- aure aureuu*al,nella ;+ ?@ -,luti,n *teril rile t,, t,,thi(Beaer   Cr"-tal i,let
M"0(* 9 *ear r rearati,n ;btain a (lean -lie an iie it int, ? eual art-. la(e a r, , 5ater in the ile , ea(h art U-ing a-eti( te(hniue4 -ear a l,,ul , ba(terial -ale r, a ure (ulture ,nt, ea(h art , the -lie. *ear the -ale in a (ir(le ,n ea(h art , the -lie Let the rearati,n ,n the -lie r". 9 ing agent Fal(,h,l : a(et,neGuntil -tain n, l,nger 5a-he- r, the -lie 5hen hel at an angle. Hui(l" rin-e -lie 5ith 5ater. l,, -lie 5ith -aranin. All,5 -tain t, reain ,r ?0 -e(,n-. Ein-e 5ith 5ater an rain. Bl,t -lie 5ithin a a , bl,tting aer. %, n,t rub. The -lie -h,ul be (,letel" r" be,re i(r,-(,i( eainati,n. ;b-ere ba(terial (ell- at 41041004 an 1000 agnii(ati,n. • • •

• •

•

• • • •

• •

• •

•

9 U-ing U-ing ;+ ;+ a- an an ini( ini(at, at,rr , gra -ta -tain in rea( rea(ti, ti,nn ;btain a (lean -lie an iie it int, ? eual art-. la(e a r, , ?@ ;+ in the ile , ea(h art U-ing a-eti( te(hniue4 -ear a l,,ul , ba(terial -ale r, the (ulture eiu t, the r,. )i the ba(teria int, the -,luti,n rail" an in a (ir(ular ,ti,n Eai-e an l,5er the t,,thi( ater 9J -e(,n- t, eterine the -tringing ee(t. I there i- -tringing 5hi(h ean- that in(rea-ing the i-(,-it" 5ithin 1 -e(,n-4 the ba(teria are (,n-iere t, be
•

•


2. S"# S"#%0 %0$( $(!(! !(!!u !u #u&u #u&u



V .

DISCUSSION

Ater