Rationale for Using the Miller Tainter Method

Rationale for Using the the Miller Miller Tainter Tainter Method and the Intraperitoneal Route of Drug Administration

Miller Tainter Method of Toxicity Testing

 percentage

mortality was converted into probability units (probits) by referring to appropriate tables

Miller Tainter Method of Toxicity Testing

 Obtained probit values are then plotted

against log-doses to get a log-dose probit graph

Miller Tainter Method of Toxicity Testing

 Probit 5 (equivalent to 50%mortality) on the Y-

axis is interpolated to the X-axis to get logLD50, the antilogarithm of which gives LD 50

Advantage and Disadvantage Advantage:  In certain instances, it has been considered to

express the standard errors of ED 50 values in terms of per cent of the ED50. In these cases the standard errors have been approximated by the method proposed by Miller and Tainter

Advantage and Disadvantage Disadvantage:  Too many animals were utilized  Expensive  Inaccuracy of results

Comparison in the Different Methods

in Obtaining the LD50

Route of Administration Intraperitoneal Route Advantage:  The LD50 value depends on the route of  

administration. The values are found to increase in the following sequence: intravenous>intraperitoneal>subcutaneous>oral

 easy to perform

Route of Administration Disadvantage:  issues of absorption, misplacement and

irritation are common  visceral absorption varies

Route of Administration  drug will enter the portal vein and may be

subject to first-pass metabolism in the liver  irritation (eg. barbiturates) may affect the

absorption rate and , and may also cause peritoneal and visceral pain