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OBSERVING MONOCOT AND DICOT STEMS
Purpose: To see the difference between the dicot and monocot stem.
Examine one half of the carrot, and look stele and food storing cortex.
Cut a very slim piece of carrot.
Put a microscope slide at the top of the beaker, and put the carrot piece top it.
Drop methylene blue on the carrot by using dropper.
Wait about 2 minutes. But don't forget it shouldn't be more than 2-3 minutes.
Then take the slide and place it to the microscope.
Set the objective correctly.
Observe the cross section of the carrot by using microscope.
Observation:
Procedure 2:
Place the slide of the sunflower and corn root under the microscope.
Look all the parts of them.
Match them by each other.
Observation 2:
CORN ROOT
SUNFLOWER ROOT
Procedure 3:
Observe the sunflower and corn stem by using microscope.
Match each other.
Observation:
SUNFLOWER STEM
CORN STEM
Conclusion:
In dicot stem you can see multicellular stem hairs easily, and you can distinguish cortex well. Endodermis and pericycle layers are present. Vascular bundles are conjoint and open. Pith is distinct and centrally located. In monocot stem, it is hard to see stem hair. No cortex, the entire tissue below hyperdermis is ground tissue, no endodermis of pericycle. Vascular bundles are messy in the ground tissue, they are conjoint, and closed. Pith can not distinguish easily.
When we observe the carrot cross section under the microscope, there was still methylene blue marks on the cross section. There are two reasons of it. One of them is we didn't use alcohol for to wash methylene blue. The other is we waited a little more than normal.
If we applied the best procedure, we could observe the things better. And maybe if we use some different plants also it would be better to get parallel veins.
