Group 8 - Microbiology 1 _Bacteriology

PHILIPPINE ASSOCIATION OF SCHOOLS OF MEDICAL TECHNOLOGY AND PUBLIC HEALTH Prototype Course Syllabus

Course Title: Microbiology 1 (Bacteriology)

Course Description: The course covers the morphology and physiology of bacteria, rickettsiae and other significant atypical bacteria and their role in infection and immunity. Emphasis is made on their isolation and identification and susceptibility testing as an aid in laboratory diagnosis.

Course Credit: 5 units (3 units lecture, 2 units laboratory)

Contact Hours: 3 hours lecture and 6 hours laboratory per week (54 hours lecture and 108 hours laboratory per semester)

Prerequisites: Biochemistry, Human Anatomy and Physiology st

Placement: Third Year, 1 Semester

Terminal Competencies: At the end of this course, the student is able to: 1. identify bacteria according to standard laboratory techniques: microscopic and staining; growth and biochemical characteristics; and antigen typing. 2. perform appropriate antimicrobial susceptibility testing 3. operate common instruments used in Bacteriology 4. participate in quality assurance/quality control program in Microbiology 5. understand the duties and responsibilities of medical technologists in the clinical setting 6. perform proper specimen collection, processing, transport and waste disposal 7. apply concepts in controlling the growth and spread of pathogenic bacteria

References: th 1. Alcamo, Edward I. Fundamentals of Microbiology 6 ed. Boston: Jones & Bartlett Publishers, 2001. 2. Bartlet, Margaret A. Diagnostic Bacteriology: A Study Guide. F.A. Davis Co. Philadelphia, USA, 2000. th 3. Black, Jacquelyn. Microbiology: Principles and Explorations 7 ed. USA: Wiley, 2008. th 4. Brooks, Geoff. Jawetz, Melnick & Adelberg’s Medical Microbiology 24 ed. Boston: Mc Graw Hill, 2007. th 5. Burton, Gwendolyn and Paul Engelkirk. Burton’s Microbiology for the Health Sciences 8 ed. USA: Lippincott Williams and Wilkins, 2007. th 6. Forbes, Betty, Daniel Salm and Alice Weissfield. Bailey & Scott’s Diagnostic Microbiology 12 ed. USA: Mosby, 2007. PASMETH, Inc. Copyright © 2008 All Rights Reserved.

7. 8. 9. 10. 11. 12. 13. 14. 15. 16.

Funke, Berdell. Microbiology: An Introduction. USA: Addison Wesley Publishing Co., 2007. th Gladwin, Mark and Bill Trattler. Clinical Microbiology Made Ridiculously Simple 4 ed. USA: MedMaster, Inc., 2007. nd Hawkey, Peter and Dierdre Lewis. Medical Bacteriology: A Practical Approach 2 ed. USA: Oxford University Press, 2004. nd McClatchey, Kenneth. Clinical Laboratory Medicine 2 ed. USA: Lippincott Williams and Wilkins, 2002. st Mcpherson, Richard A. and Matthew R. Pincus. Henry’s Clinical Diagnosis and Management by Laboratory Methods 21 ed. Philadelphia: Elsevier Inc., 2007. th Murray, Patrick R. et. al. Medical Microbiology 5 ed. USA: Mosby, 2005. th Pommerville, Jeffrey. Alcamo’s Fundamentals of Microbiology 8 ed. USA: Jones and Bartlett Publishers, 2006. th Presscott, L., J. Harley and D. Klein. Microbiology 6 ed. USA: McGraw-Hill Science, 2004. th Tortora, G., B. Funke and C. Case. Microbiology: An Introduction 9 ed. USA: Benjamin Cummings, 2006. nd Vandepitte J., J. Verhaegen and K. Engbaek. Basic Laboratory Procedures in Clinical Bacteriology 2 ed. World Health Organization, 2003.

Electronic References: 1. http://connection.lwww.com/go/burton/7e 2. http://medic.med.uth.tmc.edu/path/00001450.htm 3. http://www.kensbiorefs.com/Microbio.html 4. http://www.microbes.info 5. http://www.splammo.net/JLbactsite.html

PASMETH, Inc. Copyright © 2008 All Rights Reserved.

Learning Objectives / Intermediate Competencies

T.A. Content

At the end of this unit, the student is I. Introduction to Microbiology Lecture: able to: 1. Definition of terms: 3. Define terms. 1.1. Bacteriology, bacteria, 4. Enumerate the development of pathogenecity, virulence etc. science with emphasis on person/scientists and their 2. Historical Development 3. Divisions of Microbiology contributions 5. Explain the divisions of Microbiology Laboratory: 6. Discuss general considerations 1. Expectations/requirements Microbiology regarding proper handling, Laboratory: processing, storage of 2. Rules to follow in handling, processing, specimens and disposal of disposal of specimens/waste in wastes Microbiology laboratory 7. Discuss the roles of a medical 3. Barrier precautions technologist in a microbiology 4. Proper use of different apparatus, laboratory materials and equipment: Petri Dish, inoculating loop and needle 5. Review use and care of microscope At the end of this unit, the student is II. Laboratory Safety and Infection Control Lecture: able to: 1. Sterilization 1. Define terms 1.1. Physical (Heat) 2. Discuss concepts, principles& 1.1.1. Autoclave significance of infection control 1.1.2. Direct Heat & laboratory safety 3. Discuss various methods of 1.1.3. Oven sterilization & disinfections with 1.1.4. Incineration emphasis on temp, time, 1.1.5. Pasteurization principles/mechanism involved, 1.1.6. Boiling when to use; advantages & 1.2. Filtration disadvantages 2. Disinfection 4. Classify the different biosafety 2.1. Chemicals: cabinets 2.2. Alcohol 5. Explain the significance of 2.3. Chlorine pathogenic and virulence 2.4. Others factors in the development of 3. Antimicrobial agents infections 4. Antimicrobial Susceptibility testing 6. Perform correctly sterilization & 4.1. Automated disinfection practices 4.2. Diffusion 7. Explain the concepts behind 4.3. Dilution Antimicrobial Susceptibility 5. Use of bio-safety cabinets testing 6. Barrier precautions 8. Differentiate between the two 7. Control of nosocomial infections

Teaching Strategies Lecture

2 hrs

Values

Evaluation/Assessment

Laboratory

Large group discussion PowerPoint presentation

Demonstration & return demonstration Performance of tests

Technical Skills: Use of Microscope Safety precautions in the laboratory Specimen handling Waste disposal

Adherence to rules Responsibility Accountability Cooperation

Oral and Written Exam Participation during discussions Quality of assignment submitted Performance checklist

Lecture with demonstration Discussion Board work Case Presentation

Performance of Tests Demonstration and return demonstration

Technical Skills Multi-tasking

Honesty Commitment Responsibility Accountability Adherence to rules Cooperation Teamwork Concern for others and the environment

Oral and Written Exam Participation during discussions Quality of assignment submitted Performance checklist Practical Exam

6 hrs

3 hrs

Skills


types of antimicrobial susceptibility testing 9. Discuss significance advantages and disadvantages of each method of sensitivity test 10. Interpret test results 11. Explain the concepts of nosocomial infections 12. Outline the roles of med tech in prevention & control of nosocomial infections At the end of this unit, the student is able to: 1. Describe bacteria according to: physiology, replication, morphology, classification 2. Identify the growth requirements for each particular group of bacteria 3. Illustrate and discuss the growth curve with emphasis on the events happening per stage 4. Discuss specimen collection, transport, storage, and processing 5. Perform correctly specimen collection: Swabs, Nasal, Throat, Wound, Urine; transport & labeling, processing & waste disposal 6. Correlate specimen with common pathogens isolated 7. Perform correctly hanging drop and wet mount techniques. 8. Describe the results of hanging drop and wet mounts 9. Discuss concepts and principles of the techniques by which bacteria are identified in the laboratory 10. Correlate methods with test results 11. Prepare a general schema for identification of bacteria in the laboratory

Laboratory: 1. Sterilization: 1.1. Autoclaving 1.2. Direct heating 1.3. Filtration 2. Cleaning of working area with Clorox & other chemicals 3. Proper hand washing techniques 4. Lab exercises on the effects of chemicals & temperature on bacteria 5. Proper working attire in a Bacteriology laboratory III. Bacterial Structure and Function, Growth and Nutrition Lecture: 1. Characteristics of Bacteria according to: 1.1. Physiology: Structure, Function 1.2. Bacterial morphology 1.3. Classification 1.4. Replication 1.5. Growth requirements 1.5.1. Physical 1.5.2. Nutritional 2. Bacterial Growth Curve 3. Bacterial Identification 3.1. Microscopy: Morphology 3.2. Cultural/Colony Characteristics 3.3. Staining Characteristics 3.4. Biochemical 3.5. Serological: Antigen testing 3.6. Molecular Techniques 4. Quality Assurance-Quality Control

6 hrs

Laboratory: 1. Acceptable specimens for bacterial identification 1.1. Swabs 1.2. Blood 1.3. Urine 1.4. Stool 1.5. Tissue 1.6. Aspirates 1.7. Others 2. Specimen collection, handling, transport, processing and preservation 3. Hanging Drop Method and Wet Mounts

9 hrs

3 hrs

Computer-aided Lecture /Discussion Case studies Problem solving

Actual performance Demonstration/return demonstration Discussion of test results Making conclusions & guidelines Multi-tasking

Technical Skills Multi-tasking Problem-Solving Skills Communication skills

Honesty Commitment Responsibility Accountability Adherence to rules Cooperation Teamwork



12. Describe microscopic morphology 13. Identify correctly bacterial reaction to staining and morphology 14. Describe staining and morphological characteristics 15. Differentiate staining techniques according to: stains used, purpose, principles, advantages, disadvantages, and procedures 16. Perform laboratory exercises correctly 17. Write a report based on accepted standard format

At the end of this unit, the student is able to: 1. Discuss the concepts of bacterial genetics and its importance in the study of bacteria. 2. Appreciate the role of medical technologists in the control and prevention of microbial resistance At the end of this unit, the student is able to: 1. Define terms. 2. Discuss the mode of transmission of bacterial infections 3. Correlate factors of pathogenecity and virulence

At the end of this unit, the student is able to: 1. Define terms 2. Identify body sites with normal flora 3. Discuss the predominant normal

4.

Role of Microscopy in Bacterial Identification 4.1. Bright field 4.2. Phase contrast 4.3. Electron 4.4. Dark-field 4.5. Fluorescent 5. Preparation of Bacterial Smear for staining from: 5.1. Directly from specimen (throat swab or any swab) 5.2. Colonies growing in culture media 6. Staining Techniques: 6.1. Simple stain 6.2. Differential stain 6.2.1. Gram stain 6.2.2. Acid fast stain 6.3. Special stains 6.4. Others 7. Laboratory Safety 8. Laboratory Waste Management IV. Bacterial Genetics Lecture: 1. Basic Concepts 1.1. Bacterial DNA & RNA 1.2. Plasmids & Microbial Resistance 1.3. DNA exchange in bacteria: 1.3.1. Transformation 1.3.2. Transduction 1.3.3. Conjugation V. Pathogenesis and Epidemiology Lecture: 1. Basic Concepts 2. Mode of Transmission 3. Factors associated with pathogenecity and virulence 3.1. Toxins 3.2. Enzymes 3.3. Capsules 3.4. Others VI. Normal Flora Lecture: 1. Basic Concepts 2. Body Sites with Normal Flora 3. Predominant flora 4. Beneficial and harmful effects of normal

2 hrs

2 hrs

2 hrs

Computer-aided Lecture /Discussion Case studies Problem solving Output presentation and critiquing

Multi-tasking Problem-Solving Skills Presentation Skills Time management Communication skills

Honesty Commitment Responsibility Accountability Adherence to rules Cooperation Teamwork Prudence

Oral and Written Exam Participation during discussions Quality of assignment submitted

Computer-aided Lecture /Discussion Case studies Problem solving

Multi-tasking Problem-Solving Skills Presentation Skills Time management Communication skills

Honesty Commitment Responsibility Accountability Adherence to rules Cooperation Teamwork Prudence

Oral and Written Exam Participation during discussions Quality of assignment submitted

Technical Skills: Use of Microscope Safety precautions in the laboratory

Adherence to rules Responsibility Accountability Cooperation

Oral and Written Exam Participation during discussions Quality of assignment submitted Performance checklist

Large group discussion PowerPoint presentation

Demonstration & return demonstration Performance of tests


4.

flora in each body site Explain the beneficial and harmful effects of normal flora

At the end of this unit, the student is able to: 1. Describe Micrococcaceae & Streptococcus in terms of their general properties and pathogenesis 2. Discuss the identification characteristics of Micrococaceae in terms of morphology, culture media, colony characteristics, biochemical test, and serological test 3. Explain the principles/concepts of different identification techniques used 4. Different pathogenic from nonpathogenic members of the genus 5. Enumerate the appropriate specimen submitted in the laboratory for isolation 6. Prepare a schematic diagram for identification 7. Classify culture media according to: indications for use, contents, purpose, etc. 8. Prepare culture media 9. Perform quality control of culture media before use. 10. Identify Staphylococcus, Micrococcus and Streptococcus from specimens

At the end of this unit, the student is able to: 1. Describe the members of

flora Laboratory 1. Isolation of normal flora 2. Laboratory Safety 3. Laboratory Waste Management VII. Micrococcaceae and Streptococcaeae 1. Micrococcaceae 1.1. Staphylococcus 1.2. Micrococcus 2. Streptococcaceae 2.1. Streptococcus 2.2. Entererococcus

6 hrs

Lecture: 1. General characteristics 2. Pathogenesis & spectrum of disease 3. Laboratory Identification 3.3. Specimen used for Isolation 3.4. Morphology & Staining Reaction 3.5. Culture media and colony characteristics 3.6. Biochemical tests 3.7. Antigen typing/serology 4. Prevention and control

3.5 hrs

Laboratory: 1. Culture media 1.1. classification 1.2. composition 1.3. preparation 1.4. storage 1.5. quality assurance/control 1.5.1. spore strip test 1.5.2. sterility test 2. Preparation of culture media 2.1. Nutrient Agar 2.2. Blood Agar Plate 2.3. Chocolate Agar Plate 3. Identification of Staphylococcus and Streptococcus from specimens, prepared slides and visual aids 4. Laboratory Safety 5. Laboratory Waste Management VIII. Neisseriaceae Lecture: 1. Neisseriaceae

6 hrs

Specimen handling Waste disposal

Lecture with demonstration Multimedia presentation Discussion Board work Case Studies





Lecture with demonstration Multimedia

Performance of Tests Demonstration and

Technical Skills: Use of Microscope

Honesty Commitment Responsibility

Oral and Written Exam Participation during discussions PASMETH, Inc. Copyright © 2008 All Rights Reserved.

Neisseriaceae in terms of their general properties and pathogenesis 2. Discuss the identification characteristics of Neisseriaceae in terms of morphology, culture media, colony characteristics, biochemical test, and serological test 3. Explain the principles/concepts of different identification techniques used 4. Different pathogenic from nonpathogenic members of the genus 5. Enumerate the appropriate specimen submitted in the laboratory isolation & identification 6. Prepare a schematic diagram for identification 7. Classify culture media according to: indications for use, contents, purpose, etc. 8. Perform correctly inoculation techniques and tests 9. Summarize, based on results of identification techniques, bacterial flora in a human person and environment At the end of this unit, the student is able to: 1. Discuss the process of identification of Enterobacteriaceae in terms of morphology, culture media, colony characteristics, biochemical test, serological test 2. Explain the principles/concepts of different identification techniques used 3. Different pathogenic from nonpathogenic members of the genus 4. State the pathogenic and

1.1. Pathogenic Neisseria 1.2. Non-pathogenic Neisseria 1.3. Moraxella catarrhalis Lecture: 1. General characteristics 2. Pathogenesis & spectrum of disease 3. Laboratory Identification 3.1. Specimen used for Isolation 3.2. Morphology & Staining Reaction 3.3. Culture media and colony characteristics 3.4. Biochemical tests 3.5. Antigen typing/serology 4. Prevention and control Laboratory: 1. Identification techniques for: 1.1. Pathogenic Neisseria 1.2. Non-pathogenic Neisseria 1.3. Moraxella catarrhalis 2. Identification of species using prepared slides and visual aids 3. Laboratory Safety 4. Laboratory Waste Management

IX. Enterobacteriaceae 1. True Intestinal Pathogens 1.1. Salmonella 1.2. Shigella 1.3. Yersinia 1.4. Escherichia 2. Opportunistic pathogens 2.1. Escherichia 2.2. Citrobacter 2.3. Proteus 2.4. Edwardsiella 2.5. Morganella 2.6. Hafnia 2.7. Providencia 2.8. Enterobacter 2.9. Serratia

presentation Discussion Board work Case Studies

return demonstration

Safety precautions in the laboratory Specimen handling Waste disposal

Accountability Adherence to rules Cooperation Teamwork Concern for others and the environment

Quality of assignment submitted Performance checklist Practical Exam






3.5 hrs

6 hrs


virulence factors present in bacteria belonging to the group and disease caused by them 5. Enumerate the appropriate specimen submitted in the laboratory isolation & identification 6. Prepare a schematic diagram for identification 7. Classify culture media according to: indications for use, contents, purpose, etc. 8. Properly inoculate specimen on culture media 9. Summarize, based on results of identification techniques, bacterial flora in a human person and environment 10. Perform identification techniques 11. Identify the isolate At the end of this unit, the student is able to: 1. Discuss the process of identification of the members of the non-enteric gastrointestinal pathogens in terms of morphology, culture media, colony characteristics, biochemical test (plus positive and negative results), serological test 2. Explain the principles/concepts of different identification techniques used 3. Different pathogenic from nonpathogenic members of the genus 4. Enumerate the appropriate specimen submitted in the laboratory isolation & identification 5. Construct a schematic diagram for identification.

2.10. Klebsiella. 2.11. Others Lecture: 1. General characteristics 2. Pathogenesis & spectrum of disease 3. Laboratory Identification 3.1. Specimen used for Isolation 3.2. Morphology & Staining Reaction 3.3. Culture media and colony characteristics 3.4. Biochemical tests 3.5. Antigen typing/serology 4. Prevention and control

3.5 hrs

Laboratory: 1. Isolation and identification of members of Enterobacteriaceae using urine or stool 2. Identification of species using prepared slides and visual aids 3. Laboratory Safety 4. Laboratory Waste Management X. Non-enteric Gastrointestinal Pathogens 1. Vibrio 2. Campylobacter 3. Helicobacter 4. Aeromonas 5. Pleisomonas 6. Others

6 hrs


3.5 hrs

Laboratory: 1. Isolation and identification of members of non-enteric gastrointestinal pathogens 2. Identification of species using prepared slides and visual aids

6 hrs







At the end of this unit, the student is able to: 1. Discuss the process identification of the nonfermentative group in terms of morphology, culture media, colony characteristics, biochemical test(plus positive and negative results), serological test 2. Explain the principles/concepts of different identification techniques used 3. Different pathogenic from nonpathogenic members of the genus 4. Enumerate the appropriate specimen submitted in the laboratory isolation & identification 5. Prepare a schematic diagram for identification 6. Perform correctly inoculation techniques and tests

At the end of this unit, the student is able to: 1. Discuss the process of identification of small pleomorphic gram negative

3. Laboratory Safety 4. Laboratory Waste Management XI. Non-fermentative Gram Negative Bacilli and Aerobic Gram Positive Bacilli 1. Non-fermentative Gram Negative Bacilli 1.1. Pseudomonas 1.2. Acinetobacter 1.3. Flavobacterium 1.4. Burkholderia 1.5. Alcaligenes 1.6. Achromobacter 1.7. Stenotrophomonas 1.8. Others 2. Aerobic Gram Positive Bacilli 2.1. Bacillus 2.2. Listeria 2.3. Corynebacterium 2.4. Erysipelothrix 2.5. Nocardia 2.6. Streptomyces 2.7. Others Lecture: 1. General characteristics 2. Pathogenesis & spectrum of disease 3. Laboratory Identification 3.1. Specimen used for Isolation 3.2. Morphology & Staining Reaction 3.3. Culture media and colony characteristics 3.4. Biochemical tests 3.5. Antigen typing/serology 4. Prevention and control

3.5 hrs

Laboratory: 1. Isolation and identification of members of non-fermentative gram negative bacilli and aerobic gram positive bacilli 2. Identification of species using prepared slides and visual aids 3. Laboratory Safety 4. Laboratory Waste Management XII. Small, Pleomorphic Gram Negative Bacilli 1. Hemophilus 2. Bordetella 3. Brucella 4. Francisella

6 hrs






Lecture with demonstration Multimedia presentation Discussion


Technical Skills: Use of Microscope Safety precautions in

Honesty Commitment Responsibility Accountability Adherence to

Oral and Written Exam Participation during discussions Quality of assignment submitted PASMETH, Inc. Copyright © 2008 All Rights Reserved.

bacilli and the aerobic gram positive bacilli in terms of morphology, culture media, colony characteristics, biochemical test (plus positive and negative results), serological test 2. Explain the principles/concepts of different identification techniques used 3. Different pathogenic from nonpathogenic members of the genus 4. State the pathogenic and virulence factors present in bacteria belonging to the group and disease caused by them 5. Determine the appropriateness of the different specimens submitted in the laboratory for isolation & identification 6. Prepare a schematic diagram for identification 7. To distinguish the species of the aerobic gram positive bacilli from other bacteria 8. Identify members from submitted sample At the end of this unit, the student is able to: 1. Discuss the process of identification of Mycobacteria in terms of morphology, culture media, colony characteristics, biochemical test (plus positive and negative results), serological test 2. Explain the principles/concepts of different identification techniques used 3. Different pathogenic from nonpathogenic members of the genus 4. State the pathogenic and virulence factors present in bacteria belonging to the group and disease caused by them

5. Gardnerella 6. Legionella 7. Others

Board work Case Studies


3.5 hrs

Laboratory: 1. Isolation and identification of members of small, pleomorphic gram negative bacilli 2. Identification of species using prepared slides and visual aids 3. Laboratory Safety 4. Laboratory Waste Management

6 hrs

XIII. Mycobacterium Group 1. Mycobacterium tuberculosis Complex 2. Non-tuberculous Mycobacteria 3. Mycobacterium leprae Lecture: 1. General characteristics 2. Pathogenesis & spectrum of disease 3. Laboratory Identification 3.1. Specimen used for Isolation 3.2. Morphology & Staining Reaction 3.3. Culture media and colony characteristics 3.4. Biochemical tests 3.5. Antigen typing/serology 4. Prevention and control 4.1. TB-DOTS

3.5 hrs

Laboratory:

6



the laboratory Specimen handling Waste disposal

rules Cooperation Teamwork Concern for others and the environment

Performance checklist Practical Exam





Isolation and identification of members of Mycobacteria 1.1. Culture 1.2. Staining 1.2.1. Acid Fast Staining 1.2.2. Other Staining Techniques 1.3. Others 2. Identification of species using prepared slides and visual aids 3. Laboratory Safety 4. Laboratory Waste Management XIV. Anaerobic Bacteria 1. Clostridium 2. Bacteroides 3. Actinomyces 4. Fusobacterium 5. Peptostreptococcus 6. Porphyromonas 7. Bifidobacterium 8. Lactobacillus 9. Veilonella 10. Megasphaera 11. Acidominococcus 12. Others

hrs

Lecture: 1. General characteristics 2. Pathogenesis & spectrum of disease 3. Laboratory Identification 3.1. Specimen collection and processing 3.2. Anaerobic set-up and culture media 4. Prevention and control

3.5 hrs

Laboratory: 1. Isolation and identification of members of anaerobic bacteria 1.1. Anaerobic culture 1.2. Anaerobic set-up 1.3. Microscopic identification 2. Identification of species using prepared slides and visual aids 3. Laboratory Safety 4. Laboratory Waste Management At the end of this unit, the student is XV. Spirochetes 1. Treponema able to: 1. Discuss the process of 2. Borrelia identification of medically 3. Leptospira

6 hrs

5.

6. 7. 8.

Determine the appropriateness of the different specimens submitted in the laboratory for isolation & identification Prepare a schematic diagram for identification To distinguish the species Identify members from of the gram positive bacilli from other bacteria submitted sample

At the end of this unit, the student is able to: 1. Enumerate the characteristics of the anaerobic bacteria in terms of metabolism/physiology 2. Explain precautions of the collection, processing, handling, disposal of specimen 3. Discuss the clinically significance anaerobic in terms of morphology, isolation techniques, biochemical tests 4. Perform the set-up of anaerobic bacteriology 5. Discuss the importance of the components in the set-up

1.






Lecture with demonstration Multimedia presentation


Technical Skills: Use of Microscope Safety

Honesty Commitment Responsibility Accountability

Oral and Written Exam Participation during discussions Quality of assignment PASMETH, Inc. Copyright © 2008 All Rights Reserved.

important Spirochetes Correlate appropriate specimen with isolates 3. Characterize the organisms according to morphology and pathophysiology 4. Perform the set-up of anaerobic bacteriology 5. Discuss the importance of the components in the set-up 6. Perform correctly staining techniques for Spirochetes 7. Describe Spirochetes observed under the microscope At the end of this unit, the student is able to: 1. Discuss the process of identification of unique bacteria 2. Explain the pathogenesis and spectrum of diseases caused by unique bacteria 3. Discuss the mode of transmission of the microorganisms 4. Explain the principles behind the tests employed to identify unique bacteria 5. Perform tests to identify unique bacteria 2.

At the end of this unit, the student is able to: 1. Discuss the process of identification of miscellaneous bacteria 2. Explain the pathogenesis and spectrum of diseases caused by miscellaneous bacteria 3. Discuss the mode of transmission of the microorganisms 4. Explain the principles behind

Lecture: 1. General characteristics 2. Pathogenesis & spectrum of disease 3. Laboratory Identification 4. Prevention and control

3 hrs

Laboratory: 1. Staining techniques for Spirochetes 2. Identification of species using visual aids 3. Laboratory Safety 4. Laboratory Waste Management

6 hrs

XVI. Unique Bacteria 1. Rickettsiaceae 3.1. Rickettsia 3.2. Ehrlichia 3.3. Orientia 4. Mycoplasmataceae 4.1. Mycoplasma 4.2. Ureaplasma 5. Chlamydiaceae 5.1. Chlamydia 5.2. Chlamydophila Lecture: 1. General characteristics 2. Pathogenesis & spectrum of disease 3. Laboratory Identification 4. Prevention and control

3 hrs

Laboratory: 1. Laboratory work-up on the identification of unique bacteria XVII. Miscellaneous Bacteria 1. Eikinella 2. Kingella 3. Streptobacillus 4. Bartonella 5. Spirillum 6. Others

6 hrs

Lecture: 1. General characteristics 2. Pathogenesis & spectrum of disease 3. Laboratory Identification

3 hrs

Discussion Board work Case Studies

precautions in the laboratory Specimen handling Waste disposal

Adherence to rules Cooperation Teamwork Concern for others and the environment

submitted Performance checklist Practical Exam












5.

the tests employed to identify miscellaneous bacteria Perform tests to identify miscellaneous bacteria

At the end of this unit, the student is able to: 1. Prepare media for the finale examination 2. Perform correctly the procedures in water, food and milk analysis 3. Interpret the results of the analysis

4.

Prevention and control

Laboratory: 1. Isolation and identification of members of miscellaneous bacteria 2. Identification of species using prepared slides and visual aids XVIII. Applied Bacteriology Lecture: 1. Applied Bacteriology 1.1. Water Bacteriology 1.2. Food Bacteriology 1.3. Milk Bacteriology 1.4. Environmental Bacteriology

6 hrs

Laboratory: 1. Lab. Identification of Unknown Bacteria as Practical Examination 2. Laboratory Safety 3. Laboratory Waste Management

15 hrs

3 hrs







Technical Working Group for Prototype Course Syllabi Development (2007-2008) Zennie Aceron Petrona Benitez Sergia Cacatian Zenaida Cajucom Edwin Cancino Jacinta Cruz De Carlos Leon Oliver Shane Dumaoal Bernard Ebuen Nini Lim

Frederick Llanera Carina Magbojos Gregorio Martin Fe Martinez Josephine Milan Ferdinand Mortel Magdalena Natividad Rodolfo Rabor Ma. Teresa Rodriguez Celia Roslin

Anacleta Valdez Rowen Yolo and other PASMETH members not cited in this page who in one way or another has contributed greatly to the success of this endeavor…


Group 8 - Microbiology 1 _Bacteriology

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