Determine the resonance of Spring-Dashpot System in different damping conditions.
EAT 301 - Air Pollution Engineering
Air Pollution EngineeringFull description
Preparation of Potassium Tri(oxalate)aluminate(III)trihydrateFull description
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INDUSTRIAL BIOCHEMISTRY
CLB 20103
Experiment 4 Enzymes Activity
Introduction
Enzymes are proteins that catalyze biochemical reactions. As such, enzyme activities are influence by various physical an chemical factors affectin! protein confi!uration an stability. "n a !eneralize enzyme reaction epicte belo#, the enzyme activity can be assaye by measurin! the consumption of the substrate or the formation of the prouct, or both. A
B Enzyme
$herefore, one must have appropriate methos for the measurements of either the concentration of A or B, or both. "n most cases, the measurement of either A or B #oul suffice thou!h appropriate controls #oul have to be carrie out to ma%e certain that the conversion of A to B is an enzyme catalyze reaction. Materials
2. /re&incubate all tubes at room temperature for 10 minutes. 3. $o tube 1, a 1.0 mL of 0.0- *+, vorte7, then a 0.1 mL of enzyme solution. $his is your zero&time control. -. $o tubes 2 to 6, a 0.1 mL of enzyme solution an incubate at room temperature for the allocate time as sho#n in the above table. $he incubation time is set as soon as the enzyme solution is ae to the tube. At the en of the incubation perio, a 1.0 mL of 0.0- *+ to stop the enzyme reaction ie. $o tube 2, the *+ is ae after ( minutes an for tube 3, 10 minutes an so forth. (. 8ea absorbance at -10 nm usin! tube 1 as the blan%. /lot a !raph of prouct forme versus time. 9rom the !raph, etermine the activity of the phosphatase enzyme, e7presse as :mol p&'/ forme)min)mL of enzyme. +ne unit ; of phosphatase activity is efine as the amount of enzyme that can prouce 1 :mol of p&'/ per minute.
