1.0
TITLE
Determination of Ammonia-Nitrogen and Nitrate-Nitrogen in Water and Wastewater
2.0
OBJECTIVE
To determine ammonia-nitrogen and nitrate-nitrogen in water and wastewater
3.0
INTRODUCTION
In ammo ammoni niaa-ni nitr troge ogen n test test,, ammo ammoni niaa comp compoun ounds ds comb combin inee with with chlor chlorin inee to form form monochloride. Monochloride reacts with salicylate to form -aminosalicylate. The aminosalicylate is o!idi"ed in the presence of a sodium nitroprusside catalyst to form a blue-colored compound. The blue color is mas#ed by the yellow color from the e!cess reagent present to gi$e a final green-colored solution. Test results are measured at % nm. Meanwhile, in nitrate-nitrogen nitrate-nitrogen test, cadmium metal metal reduces nitrates in the the sample to nitr nitrit ite. e. The The nitr nitrit itee ion ion reac reacts ts in an acidi acidicc medi medium um with with sulf sulfan anil ilic ic acid acid to form form an intermediate dia"onium salt. The salt couples with gentisic acid to form an amber colored solution. Test results are measured at &'( nm.
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MATERIALS & METHODOLOGY
Ammonia )yanurate *eagent +owder +illows- Ammonia alicylate *eagent +owder +illows- Nitraer Nitrate *eagent +owder +illows-/ 0A)0 pectrophotometer D*1&(( 2 D*13(( *ounded14uare sample cell, /( m5 Measuring cylinder, m5 6ea#er, ( Ml
Ammoni Ni!"o#$n %M$!o' (1))* 0.01 !o 0.)0 m#+L NH 3,N-
/.
Touch H /"o#"m . elect program 3() N* Ammoni* Si . Touch S!"!.
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7ill a round sample cell to the /( m5 mar# with sample.
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7ill another round sample cell to the /( m5 mar# with deioni"ed water 8the blan#9.
&.
Add the contents of one Ammonia alicylate +owder +illow to each cell. topper and sha#e to dissol$e the powder.
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Touch the timer icon. Touch O . A three-minute reaction period will begin.
%.
When the timer beeps, add the contents of one Ammonia )yanurate *eagent +owder +illow. topper and sha#e to dissol$e the reagent.
:.
Touch the timer icon. Touch O . A /-minute reaction period will begin. ;A green color will de$elop if ammonia-nitrogen is present.
3.
When the timer beeps, place the blan# into the cell holder.
<.
Touch $"o. The display will show= 0.00 m#+L NH3,N.
/(.
Wipe the sample and place it into the cell holder.
//.
Touch R$'. *esults will appear in mg15 N0'-N.
Ni!"!$ %M$!o' (11* MR 0.1 !o 10.0 m#+L NO 3,,N-
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Touch H /"o#"m . elect program 3)3 N* Ni!"!$ MR . Touch S!"!.
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7ill a round sample cell with /( m5 of sample.
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Add the contents of one Nitraer Nitrate *eagent +owder +illow 8the prepared sample9 and cap the cell.
&.
Touch the timer icon. Touch >?. A one-minute reaction period will begin. ha#e the cell $igorously until the timer beeps.
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When the timer beeps, touch the timer icon. Touch O . A fi$e-minute reaction period will begin. ;An amber color will de$elop if nitrate is present.
%.
When the timer beeps, fill a second round sample cell with /( m5 of sample 8the blan#9.
:.
+lace the blan# into the cell holder.
3.
Touch $"o. The display will show= 0.0 m#+L NO3,,N.
<.
+lace the prepared sample into the cell holder. Touch *ead. *esults will appear in mg15 N>'--N.
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RESULTS & DISCUSSIONS
ample 8different sources9
N0'-N, mg15
N>'--N, mg15
@our discussion may include the following 4uestions= /. )ompare and e!plain the $alue of ammonia nitrogen and nitrate ion nitrogen in the difference sources of sample.
5.0
.
Identify the sources of ammonia nitrogen in water and wastewater.
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!plain the biochemical reactions that can remo$e nitrate from water.
RE6ERENCES
0oward, . +., Donald, *. *. B Ceorge, T. /<3. n$ironmental ngineering. McCraw0ill. ingapore.
?asmawati, M. B 5ee, ?. ?. ((:. Methods of Analysis for Water and Wastewater. iTM. hah Alam.
D*1&(( pectrophotometer +rocedure Manual. ((&. 0ach )ompany. A.
