In this work the authors have proposed a simple, specific, economic and accurate reverse phase liquid chromatographic method for the estimation of Zolmitriptan as an active pharmaceutical ingredient and in pharmaceutical formulation. The main objecti
Description complète
SoundtrackFull description
SugarDescription complète
valve, marine, shipFull description
Full description
GSM interference reduction
ghjfdjhgkgjlkFull description
Full description
A Cost Estimation for Construction Projects
estimate welding cost
estimation de durée des travaux d'inspection
Handbook of Cane Sugar EngineeringFull description
Handbook of Cane Sugar EngineeringDescrição completa
Handbook of Cane Sugar EngineeringDescription complète
process costing of sugar industryFull description
Estimation of reducing sugar by NelsonSomogyi method Michał Kaczmarek
Keywords: reducing sugars, Somogyi-Nelson assay, equipment required: spectrophotometer capable of measuring absorbance in the 520 nm region. cuvettes for spectrophotometer water bath 100°C required reagents: carbohydrate standards (glucosamine) at the concentration ranging from 0 µmol/ml to 0,50 µmol/ml Copper reagent : 180 g Na2SO4 12 g potassium sodium tartrate 24 g Na2CO3 (anhydrous) 4 g CuSO4 x 5 H2O lub 2,6 g CuSO4 16 g NaHCO3 Preparation: Dissolve 12 g of potassium sodium tartrate and 24 g Na 2CO3 in 250 ml H2O. Followed by continuous stirring add 4 g CuSO4 x 5 H2O i 16 g NaHCO 3. Dissolve 180g of Na2SO4 in 500ml of boiling water H2O and boil to remove air bubbles. The two solutions were mixed and filtered hot to obtain a clear solution. Nelson reagent: 25 g (NH4)2MoO4 x 2H2O or 25,75 g (NH4)6Mo7O24 x 4H2O 21 cm3 H2SO4 3g AsHNa2O4 x 7 H2O Preparation : Dissolve 25 g (NH4)2MoO4 x 2H2O or 25,75 g (NH4)6Mo7O24 x 4H2O in 450 cm3 H2O and add 21 cm3 H2SO4 (concentrated) Dissolve 3g AsHNa2O4 x 7 H2O in 25 cm3 H2O Mix both reagents and incubate 30 min. in 55°C execution time: from 10 min Procedure Description: The Nelson-Somogyi method is one of the classical and widely used methods for the quantitative determination of reducing sugars. This method utilizes the reducing properties (because of the presence of a potential aldehyde or keto-groups) of certain types of carbohydrates. Determination of reducing
sugars using Somogyi-Nelson is based on the absorbance at 520 nm of a coloured complex between a copper oxidized sugar and arsenomolybdate.
Procedure: 1. Prepare carbohydrate standards in distilled water ranging from 00,50 µmol/ml. 2. Add 1 ml of each standard to separate tubes. To the tubes used as the blanks, add 1 ml of distilled water. 3. Prepare the unknown samples in an appropriate dilution. 4. To each tubes, add 1 ml of the Copper Reagent and mix. 5. Place the tubes in a boiling water for 10 minutes 6. Cool the tubes to room temperature and add 1mL of arsenomolybdate reagent – Nelson Reagent to all the tubes. 7. Measure the absorbance at 520 nm.
