DATASHEET DATASHEET FOR PRACTICAL 1 EXPERIMENT 1: THE FASTEST ENZYME – CATALASE
Table Table 1.1: Catalase react!" Test t#be c!"ta""$ C#be !) l%er -r!#"+ l%er !le+ l%er C#be !) /+"e' -r!#"+ /+"e' !le+ /+"e' C#be !) (!tat! -r!#"+ (!tat! !le+ (!tat!
Obser%at!"s *!!+e" s(l"ter l$,te+ #( *!!+e" s(l"ter l$,te+ #( *!!+e" s(l"ter +!es "!t l$,t #( *!!+e" s(l"ter +!es "!t l$,t #( *!!+e" s(l"ter l$,t #( +0l' *!!+e" s(l"ter +!es "!t l$,t #( *!!+e" s(l"ter +!es "!t l$,t #( *!!+e" s(l"ter +!es "!t l$,t #( *!!+e" s(l"ter +!es "!t l$,t #(
O&'$e" (rese"t Prese"t Prese"t Abse"t Abse"t Prese"t Abse"t Abse"t Abse"t Abse"t
Questions
1. H! ca" ca" '!# "crease "crease t,e rate rate !) catalase catalase react!" react!" b' #s"$ t,e sa0e sa0e l%er2 T,e rate !) catal'se react!" ca" be "crease+ b' "creas"$ t,e s#r)ace area !) t,e l%er. 3. H! +!es t,e te0(erat#re te0(erat#re a))ect t,e catalase react!"2 react!"2 As t,e te0(erat#re "creases4 t,e e"5'0e react!" "creases #"tl t reac,es t,e ,$,er te0(erat#re ,c, s ab!%e t,e !(t0#0 te0(erat#re a"+ t,e e"5'0e +e"at#re+. 6. Dsc#ss '!#r '!#r e&(er0e"t e&(er0e"t res#lt c!"cer""$ c!"cer""$ t,e t,ree t,ree sa0(les sa0(les #se+. #se+. 7
8s"$ l%er: Test Test t#be c!"ta""$ l%er " $r!#"+e+ a"+ c#be )!r0e+ l$,te+ #( t,e !!+e" s(l"ter ,e" t (lace+ "s+e t,e test t#be t, ,'+r!$e" (er!&+e. T,s s,!s t,at t,ere s a (rese"t (rese"t !) !&'$e" a"+ t,e e"5'0e catalase brea/s +!" ,'+r!$e" (er!&+e "t! ater a"+ !&'$e". H!e%er4 t,e rate !) e"5'0e catalase als! s a))ecte+ b' t,e te0(erat#re. !le+ l%er +!es "!t react t, ,'+r!$e" (er!&+e beca#se t,e ,eat ab!%e t,e !(t0#0 te0(erat#re ll +e"at#re !r +estr!' t,e (r!te"4 s! a b!le+ l%er sa0(le ,as +e"at#re+ catalase (r!te"s ,c, ca" "! l!"$er (er)!r0 t,er 0etab!lc )#"ct!".
7
8s"$ /+"e': T,e rate !) e"5'0e catalase react!" s a))ecte+ b' t,e s#r)ace area !) t,e /+"e'. T,at s t,e reas!" ,'4 t,e !!+e" s(l"ter +!es "!t l$,t #( )!r t,e c#be /+"e' beca#se t,ere s "! !&'$e" (r!+#ce+ a"+ t,e s#r)ace area !) t,e c#be /+"e' s s0aller t,a" t,e s#r)ace area !) t,e $r!#"+ /+"e'. -r!#"+ /+"e' l$,te+ #( t,e !!+e" s(l"ter react"$ t, t,e ,'+r!$e" (er!&+e t! (r!+#ce !&'$e" a"+ ater. !le+ /+"e' +!es "!t react t, ,'+r!$e" (er!&+e beca#se t,e (r!te" ,as bee" +e"at#re+ at te0(erat#re !) 199 ℃ .
7
8s"$ (!tat!: T,e (!tat! c!"ta"s less !) t,e e"5'0e catalase4 t,ere)!re re#res 0!re act%at!" e"er$' sl!"$ +!" t,e rate !) react!". T,at s ,'4 t,e !!+e" s(l"ter +!es "!t l$,t #( a"+ "! !&'$e" s (rese"t c!0(are+ t! l%er a"+ /+"e'.
EXPERIMENT 3: THE 8SE OF ENZYME – SOFT CENTERED CHOCOLATE
Table 1.3: e"e+ct test
Test t#be 1 3 6
e"e+ct test C!l!#r !) bl#e e"e+ct s!l#t!" re0a"s t,e sa0e C!l!#r c,a"$es t! brc/;re+ (rec(tate C!l!#r !) bl#e e"e+ct s!l#t!" re0a"s t,e sa0e
Res#lt N!";re+#c"$ s#$ar Re+#c"$ s#$ar N!";re+#c"$ s#$ar
Questions
1. *,at ,as t,e "%ertase +!"e2 I"%ertase s a carb!,'+rate;+$est"$ e"5'0e t,at s(lts s#cr!se "t! ts c!0(!"e"t (arts4 $l#c!se a"+ )r#ct!se. 3. Fr#ct!se s a re+#c"$ s#$ar – ,at test c!#l+ '!# +! !" T#bes 3 a"+ 6 t! )"+ !#t ) a re+#c"$ s#$ar s (rese"t2 e"e+ct. *,at s t,e +))ere"ce betee" s#$ar c#be c!%ere+ t, c,!c!late a"+ s#$ar c#be " test t#be. T,e (rese"ce !) c,!c!late acts as catal'st " brea/"$ +!" !) s#cr!se " s#$ar c#be.
