ANALISIS KUALITATIF BAHAN KIMIA OBAT FUROSEMID DAN HIDROKLORTIAZIDA DALAM OBAT TRADISIONAL DENGAN METODE KROMATOGRAFI LAPIS TIPIS

NINJD\D\ MPNJDUNUDB ONGNI MDKDN HONU BP_H\@KDE ENI GDE_HMJH_UDNQDEN ENJNK HONU U_NED\DHINJ E@IFNI K@UHE@ M_HKNUHF_NBD JNSD\ UDSD\

HJ@G 1  I@RDN  I@RDN SPU_D SPU_D \@MUD \@MUD F)

F>B3343 F>B3343?4 ?4

_DQMD SP\SDUN\N_D

F>B3343<>

VDKNJN S@_KNUN\N_D

F>B3343
E@ET D\MNIEN_

F>B3343<5

@MN VPJNIEN_D

F>B3>33<0

JNOH_NUH_DPK JNOH_NUH_DPK MDKDN+BN_KN\D AP_P\NI BN_KN\D BNMPJUN\ M@EHMU@_NI ENI DJKP+DJKP M@\@GNUNI PIDR@_\DUN\ A@IE@_NJ \H@ED_KNI SP_VHM@_UH

?3>?

N)

Apepj S`rlhonni Nin|jd|d| Mpnjdunudb Ongni Mdkdn Honu Bprh|`kde eni Gderhmjhrudnqden enjnk Honu Urned|dhinj e`ifni K`uhe` Mrhknuhfrnbd Jnsd| Udsd|

O) Upap Upapni ni Knksp k`jnmpmni srdi|ds ninjd|d| e`ifni k`uhe` mrhknuhfrnbd jnsd| udsd|* k`ihuhjmni |nks`j * k`if`jp|d * eni k`ifde`iudbdmn|d |`itnvn e`ifni mrhknuhfrnbd jnsd| udsd|)

L)

Njnu eni Ongni Njnu tnif edfpinmni Lgnko`r * f`jn| pmpr * sdsn mnsdj`r * sds`u hjpk` * sds`u u`u`| * khrunr |u`ks`r * bdjj`r * lhrhif * onunif s`ifnepm * |snupjn) Ongni tnif eds`rjpmni eds`rjpmni Gderhmjhrudnqden Gderhmjhrudnqden |unienr * bprh|`kde bprh|`kde |unienr * ankp lnksprni *

 ankp n|jd n|jd * k`ugnihj k`ugnihj * `udj `udj n|`unu * m`run| m`run| |nrdif * |djdln f`j) f`j)

E) Enun S`ifnknuni S`ifnknuni Anrnm tnif ed u`kspg qnu u`rjnrpu '|hjpu`$ 1  

Bprh|`kde` 1 5*0lk

 

Gderhmjhrhudnqden 1 5*? lk

 

Ankp 1 ?*? lk

 

\nks`j 1 ? lk

Fnkonr 

@) S`rgd S`rgdup upif ifni ni _b 1

Anrnm tnif edu`kspg mhkshi`i tnif jnrpu Anrnm tnif edu`ksp s`jnrpu |nksnd fnrd| brhiu

_b Gderhmjhrudnqden |unienr 1

5*? 5*? lk 1 3*75 3*75 lk 0 lk

_b Bprh|`kde |unienr

1

5*0 5*0 lk 1 3*4 3* 4 lk 0 lk

_b Ankp N|jd

1

?*? lk 1 3*55 lk 0 lk

_b Ankp Lnksprni

1

? lk

1 3*5 lk

0 lk

B) S`kongn| ngn|ni ni Mrhknu Mrhknuhfr hfrnbd nbd edfpin edfpinmni mni |`onfnd |`onfnd piupm piupm k`kd|ng k`kd|ngmni mni |po|uni |po|uni|d |d lnksprn lnksprni i k`ianed k`ianed mhkshi`i+mh mhkshi`i+mhkshi` kshi`iitn) iitn) Mrhknuhfrnbd Mrhknuhfrnbd apfn k`rpsnmni k`rpsnmni s`kd|ngni s`kd|ngni lnkprni |`itnvn |`itnvn k`ianed |`itnvn kpriditn eni k`if`ungpd mpniudun|itn) S`kd|ngni |`itnvn odn|nitn k`iffpinmni k`iffpinmni o`o`rnsn u`mgidm mrhknuhfrnbd) mrhknuhfrnbd) S`kdjdgni S`kdjdgni u`midm mrhknuhfrnbd mrhknuhfrnbd |`onfdni |`onfdni  o`|nr o`rfniupif o`rfniupif snen |dbnu m`jnrpuni m`jnrpuni |`itnvn tnif nmni edsd|ngmni) \`kpn \`kpn mrhknuhfrnbd k`kdjdmd bn|` ednk 'ensnu o`rpsn snenuni* nunp mhkodin|d lndrni+snenuni$ eni bn|` f`rnm  'o`rpsn lndrni nunp fn|$) Bn|` f`rnm k`ifnjdr k`jnjpd bn|` ednk eni k`konvn mhkshi`i+ mhkshi`i tnif u`rensnu enjnk lnksprni) Mhkshi`i+mhkshi`i tnif o`ro`en o`rf`rnm   snen jnap tnif o`ro`en o`ro`en 'Nihidk*?33 'Nihidk*?334$ 4$ Mrhknuhfrnbd Jnsd| Udsd| k`rpsnmni o`upm mrhknuhfrnbd sjninr |`jndi mrhknuhfrnbd m`run| eni `j`murhbhr`|d|) O`ro`en e`ifni mrhknuhfrnbd mhjhk tnif knin bn|` ednkitn ed d|dmni nunp edm`kn| edenjnkitn * snen mrhknuhfrnbd jnsd| udsd|* bn|` ednkitn o`rpsn jnsd|ni tnif |`rnfnk snen s`rkpmnni odenif enunr tnif edepmpif hj`g j`ks`if mnln * s`jnu njpkdidkpk * s`jnu sjn|udm 'Fnieanr D F eni Noepj _hgkni * ?336$ Bn|` f`rnm tnf edm`inj |`onfnd s`jnrpu s`if`konif nmni o`rf`rnm |`snianif bn|` ednk mnr`in s`ifnrpg mnsdj`r snen s`if`konifni |`lnrn indm 'n|l`iedif$ nunp mnr`in s`ifnrpg frndun frndun|d |d snen snen s`if` s`if`kon konifn ifni i |`lnrn |`lnrn k`iprp k`iprpi i 'e`|l`i 'e`|l`iedif edif$$ 'Fniean 'Fnieanrr D F eni Noepj Noepj _hgkni * ?336$)

Khihfrnbd Ongni >) K`unihj

K`unihj* K`unihj* apfn edm`inj |`onfnd k`udj njmhghj* njmhghj* vhhe njlhghj  nunp |sdrdup|* nenjng |`itnvn mdkdn e`ifni rpkp| mdkdn LG440 >440$) $)

?) Bprh|`kde

N|nk5+mjhrh+I+bprbprdj+0|pjbnkhtjniurnidjnu'L >?G>>LjI?H0\$ OK <<3*65) Bprh|`kden k`ifniepif udenm mprnif enrd 47*3% eni udenm j`odg enrd >3>*3 % L >?G>>LjI?H0\* edgdupi edgdupif f u`rgnen u`rgnens s qnu tnif tnif u`jng u`jng edm`rdi edm`rdifm fmni) ni) S`k`rdni S`k`rdni |`rom |`rom gnojpr* gnojpr*spu spudg dg gnks`r  gnks`r  mpidif mpidif*ude *udenm nm o`ronp o`ronp)) M`jnrpu M`jnrpuni ni srnmud| srnmud| udenm udenm *jnrpu *jnrpu ennjnk ennjnk ndr*kpe ndr*kpeng ng jnrpu jnrpu enjnk enjnk n|`uhi* n|`uhi*enj enjnk nk edk`udj edk`udj bhrknkn bhrknknden den eni enjnk enjnk jnrpuni jnrpuni njmnjd njmnjd gderhm gderhm|den |den)Jn )Jnrpu rpu enjnk enjnk k`ugnihj*nfnm k`ugnihj*nfnm |pmnr jnrpu enjnk `unihj*|pmnr `unihj*|pmnr jnrpu enjnk `u`r*|nifnu |pmnr jnrpu enjnk mjhrhbhrk 'Nihidk*>440$) <) Gderh derhm mjhru jhrudn dnqd qde e

2+mjhrh+<*5+edgderh+?G+>*?*5+O`iqhudnednqdin+6+|pjbhinkden>*>+ edhm|den'L6G7LjI3?*3 % L6G7LjI440$) 5)

@udj N|`unu

@udj N|`unu N|`unu nenjng nenjng |`itnvn hrfnidm  e`ifni rpkp| LG 440$) 0) Honu Honu urned urned|dh |dhin injj 'ankp 'ankp$$

Ankp nenjng |`opuni piupm honu urned|dhinj enrd Diehi`|dn) Diehi`|dn) O`jnmnifni shspj`r e`ifni |`opuni g`ron nunp g`ronj)Ank g`ronj)Ankp p edopnu edopnu enrd ongni+ ongni+ong ongni ni njnkd* njnkd* o`rpsn o`rpsn onfdni onfdni enrd upkopgni |`s`rud rdksnif 'nmnr+nmnrni$* enpi+enpini eni mpjdu onunif* opng) Nen apfn k`if k`iffp fpinm inmni ni ongn ongni i enrd enrd upop upopg g g`vn g`vni* i* |`s` |`s`rud rud `ks`ep mnkodif mnkodif nunp unifmp unifmpr  r   opntn)Ankp  opntn)Ankp odn|nitn u`rn|n sngdu |`gdiffn s`rjp edunkong knep |`onfnd s`knid| nfnr  rn|nitn j`odg ensnu eduhj`rni|d s`kdipkitn)Ed o`ronfnd mhun o`|nr u`rensnu srhb`|d  s`iapnj ankp f`iehif tnif o`rm`jdjdif k`iananmni k`iananmni ankp |`onfnd kdipkni tnif |`gnu eni eni k`it k`it`f `fnrm nrmni ni)) \`jnd \`jndi i dup ankp ankp apfn apfn edsr edsrhe hepm pm|d |d ed snor snordm dm+sn +snor ordm dm ankp ankp hj`g hj`g  s`rp|ngnni  s`rp|ngnni o`|nr |`s`rud Ankp Ndr Knilpr *  Ithitn  Ithitn K`i``r  K`i``r  nunp Eankp Eanfh* Eanfh* eni edapnj ed o`ronfnd uhmh honu enjnk m`kn|ni  |nlg`u ) Ankp |`s`rud did gnrp| edjnrpumni enjnk ndr snin| u`rj`odg engpjp |`o`jpk edkdipk) Snen s`rm`konifni |`jniapuitn  ankp apfn apfn edapnj edapnj enjnk o`iupm unoj`u* mnsj`u mnsj`u eni eni mns|pj mns|pj 'Nihidk*?3 'Nihidk*?334$) 34$)

Snen srnmudmpk mnjd did mnkd k`ifde`iudbdmn|d k`ifde`iudbdmn|d nen udenmitn udenmitn mniepifni bprh|`kde eni gde`hmjhrudnqde enjnk ankp s`jnif|dif tnif o`r`enr ed sn|nrni |`run ankp lnksprni tnif |`ifnan |`ifnan edlnksp edlnksprr ongni ongni mdkdn mdkdn honu honu u`r|`op u`r|`opu) u) \`enif \`enifmni mni gdrhmjh gdrhmjhrudn rudnqde qde eni bprh|`k bprh|`kde de |`onfnd |unienr) \nks`j+|nks`j uned edjnrpumni u`rj`odg engpjp e`ifni k`ugnihj) K`unihj edfpinmni mnr`in `jp`i tnif edfpinmni apfn k`ugnihj) K`unihj apfn k`rpsnmni s`jnrpu  shjnr tnif nmni k`jnrpumni k`jnrpumni ongni mdkdn uned) Gnj did |`|pnd e`ifni e`ifni |dbnu jnrpuni ‛ jdm` ed|hj` jdm` ‚ nunp k`jnrpumni |`|nkn) \`u`jng edjnrpumni |nks`j ed|nrdif u`rj`odg engpjp e`ifni upapni nfnr edensnu bdjurnu tnif a`ridg) J`ks J`ks`i `if f MJU MJU tnif tnif edfp edfpin inmn mni i nenj nenjng ng |djd |djdln ln FB?0 FB?05 5

mnr` mnr`in in |djd |djdln ln did did nmni nmni

 o`rbjphr`|`i|d  o`rbjphr`|`i|d snen |s`murh|mhsd |s`murh|mhsd snen snianif f`jhkonif f`jhkonif ?05 ik) \djdln edopnu e`ifni e`ifni pmprni 6 lk w 0 lk k`it`|pndmni udiffd eni j`onr lgnko`r) M`kpedni snen |d|d onvng edunrdm fnrd| |`udiffd > lk |`onfd fnrd| |unru tnif iniuditn nmni k`ianed u`ksnu s`ihuhjni |nks` |nks`jj eni eni enrd enrd fnrd| fnrd| |unru |unru edunrd edunrdm m |`sni |`sniani anif f 0 lk |`onf |`onfnd nd fnrd| fnrd| brhiu brhiu iniud iniudit itnn nmni nmni k`inienmni `jp|d |peng lpmps) Snen fnrd| |unru eduhuhjmni kn|dif+kn|dif |nks`j tndup |`onitnm < uhuhjni m`kpedni m`rdifmni) \`u`jng |nks`j |peng |dns*mdun opnu `jp`i tnif u`redrd enrd k`ugnihj 1 `udj n|`unu e`ifni  s`roniedifni  s`roniedifni ? 1 < tndup ? kj k`ugnihj k`ugnihj eni < kj `udj n|`unu) S`roniedifni S`roniedifni did edfpinmni edfpinmni piupm k`ks`rkpeng k`j`unmmni j`ks`if MJU mnr`in lgnko`r tnif m`ldj |`gdiffn anrnm  fnrd| brhiu eni |unru udenm u`rjnjp e`mnu nunpspi anpg) S`iffpinni S`iffpinni epn s`jnrpu did edupapmmni edupapmmni piupm k`idifmnumni gnrfn _b |`lnrn |dfidbdmni) Mnr`in |dbnu k`ugnihj tnif k`rpsnmni

 s`jnrpu shjnr eni `udj n|`unu tnif k`i`ifng k`i`ifng shjnr) @jp`i u`r|`opu u`r|`opu edkn|pmmni edkn|pmmni m` enjnk lgnko`r edupups eni edupiffp |`mdunr >3 k`idu e`ifni upapni `jp`i tnif o`rnen ed enjnk lgnko`r u`snu a`ipg m`udmn nmni edfpinmni piupm k`if`jp|d) M`kpedni |djdln f`j tnif |pen |peng g eduh eduhuh uhjm jmni ni |nks` |nks`jj edkn| edkn|pm pmmn mni i m` enjn enjnk k lgnko lgnko`r `r e`if e`ifni ni sh|d| sh|d|dd o`red o`redrd rd u`fnm  u`fnm  |`gdiffn `jp`i tnif indm nmni u`snu o`r|nkn+|nkn |nksnd edfnrd| brhiu * fnrd| |unru udenm   ohj`g k`it`iup k`it`iupg g `jp`i mnr`in mnr`in nmni k`it`ono k`it`onomni mni |nks`j |nks`j tnif eduhuhjmni eduhuhjmni jnrpu jnrpu enjnk `jp`i `jp`i eni lgnko`r edupups*edupif edupups*edupiffp fp gdiffn `jp`i k`ilnsnd k`ilnsnd fnrd| brhiu) @jp|d |`j`|nd m`udmn `jp`i k`ilns k`ilnsnd nd fnrd| fnrd| brhiu) brhiu) \djdln \djdln enjnk enjnk lgnko` lgnko`rr ednifm ednifmnu nu e`ifni e`ifni k`iff k`iffpin pinmn mn sdi|`u sdi|`u eni edm`rdi edm`rdifmn fmni) i) Mdrn+mdr Mdrn+mdrnn lpmps lpmps m`rdif m`rdif |djdln |djdln ednknud ednknud edonvn edonvng g |s`murh |s`murh|mh |mhsd sd |dinr |dinr p ?05) ?05) O`rlnm tnif u`rjdgnu edjdifmnrd e`ifni s`i|dj) M`kpedni pmpr anrnm tnif edu`kspg kn|dif+ kn|dif |nks`j) Onrp m`kpedni edkn|pmmni enjnk rpkp| _b) Gn|dj tnif eds`rhj`g _b |unienr Gderhmjhrudnqde* _b |unienr Bprh|`kde* _b ankp n|jd *  ankp lnksprni lnksprni o`ruprpu+upr o`ruprpu+uprpu pu 1 3*75 lk * 3*4 lk * 3*55 3*55 lk * 3*5 lk) Epn Epn |`itnvn |`itnvn edmnunmni edmnunmni de`idudm admn k`kspitnd k`kspitnd idjnd _b tnif |nkn admn edpmpr snen mhied|d MJU tnif |nkn spjn) Ankp n|jd tnif o`r`enr edsn|nrni u`ropmud udenm k`ifniepif ongni mdkdn honu bprh|`kde knpspi gderhmjhrudnqde edjdgnu enrd gnrfn _b tnif u`rsnpu lpmps anpg enrd gnrfn _b |unienr  bprh|`dke eni gderhmjhrudnqde) Ankp lnksprni tnif |`ifnan edlnkspr e`ifni ongni mdkdn honu gderhmjhrudnqde gderhmjhrudnqde eni bprh|`kde bprh|`kde |`gnrp|itn |`gnrp|itn k`ipiapmmn k`ipiapmmni i gnrfn _b tnif |nkn e`ifni gnrfn _b gderhmjhrudnqde eni bprh|`kde* inkpi snen srnmudmpk mnkd did udenm edu`kpmniitn gderhm gderhmjhru jhrudnqde dnqde nunpspi nunpspi bprh|`k bprh|`kde* de* mnr`in mnr`in idjnd idjnd _bitn _bitn u`rsnpu u`rsnpu lpmps lpmps anpg enrd idjnd idjnd _b  gderhm gderhmjhru jhrudnqde dnqde eni bprh|`kd bprh|`kde) e) Gnj did u`raned u`raned mnr`in mnr`in o`o`rn o`o`rnsn sn bnmuhr* bnmuhr* edniunrn edniunrnitn itn tndup tndup mhied|d mhied|d j`ks`if j`ks`if udenm enjnk m`nenno tnif onfp|* mnr`in m`udmn edjdgnu edonvng |dinr PR* u`rjdgnu fhr`|ni+fhr`|ni tnif k`iffniffp anjniitn o`rlnm |`gdiffn udenm k`ilnsnd anrnm  tnif |`gnrp|itn) Snrnk`u`r enrd u`midm mrhknuhfrnbd nenjng odjnifni _b) Odjnifni _b nenjng anrnm tnif edu`kspg edu`kspg |`itnvn snen mrhknuhfrnbd mrhknuhfrnbd u`rgnens fnrd| fnrd| e`sni) Odjnifni _b eds`rhj`g eds`rhj`g e`ifni k`ifpmpr anrnm niunrn ududm nvnj eni sp|nu o`rlnm tnif edgn|djmni |`itnvn eni anrnm did m`kpedni edonfd e`ifni anrnm niunrn ududm nvnj eni fnrd| e`sni 'anrnm tnif edu`kspg lndrni  s`if`konif  s`if`konif$$ 'Fnieanr eni _hgkni* _hgkni* ?336$) ?336$) O`o`rnsn m`piupifni mrhknuhfrnbd jnsd| udsd| nenjng |`onfnd o`rdmpu 1  

Mrhknuhfrnbd jnsd| udsd| onitnm edfpinmni piupm upapni ninjd|d|)

 

De`iudbdmn|d De`iudbdmn|d s`kd|ngni s`kd|ngni mhkshi`i mhkshi`i ensnu edjnmpmni edjnmpmni e`ifni s`r`nm|d vnrin* bjphrh|`i|d bjphrh|`i|d nunp e`ifni rnedn|d k`iffpinmni |dinr pjurndhj`u)

 

Ensnu edjnmpmni `jp|d |`lnrn k`indm ''n|l`iedif  $* k`iprpi 'e`|l`iedif  'e`|l`iedif $* $* nunp e`ifni n|l`iedif $* lnrn `jp|d ? edk`i|d)

 

M`u`snuni s`i`iupni mnenr nmni j`odg ondm mnr`in mhkshi`i tnif nmni edu`iupmni k`rpsnmni o`rlnm tnif udenm o`rf`rnm) M`mprn M`mprnifn ifni i MJU MJU nenjng nenjng nenitn nenitn bn|` f`rnm f`rnm tnif tnif m`kpri m`kpridnit dnitnn udiffd udiffd mnr`in mnr`in MJU

|nifnu |`i|dud` eni snen s`i`iupni s`ia`ipgni `jp`i enjnk lgnko`r odn|nitn mprnif njde) Mnr`in nsnodjn ed u`| e`ifni |`opng m`run| |nrdif tnif ed pnsd `jp`i knmn nmn inen penrn tnif kn|pm snen |`jn+|`jn lgnko`r tnif u`ropmn) \`gdiffn e`ifni lnrn jndi tndup e`ifni k`ifdrn+ifdrn e`ifni k`jdgnu vnmup mprnif j`odg <3 k`idu) M`j` M`j`od odgn gni i s`if s`iffp fpin inni ni MJU MJU edon edonie iedi difm fmni ni e`if e`ifni ni Mmu Mmu nenj nenjng ng mnr` mnr`in in ensn ensnuu edgn|djm edgn|djmnii niitn tn s`kd|ng s`kd|ngni ni tnif j`odg j`odg |`kspr |`ksprin in *m`s`m *m`s`mnni nni tnif tnif j`odg j`odg udiffd* udiffd*eni eni ensnu ensnu edjnm|ninmni e`ifni j`odg l`snu)Onitnm s`kd|ngni tnif k`knmni vnmup o`rank+ank odjn edm`ranmni e`ifni mrhknuhfrnbd m`run| u`unsd ensnu edjnm|ninmni gnitn o`o`rnsn k`idu |nan  odjn edm`ranmni edm`ranmni e`ifni e`ifni MJU 'Neini 'Neini*>44 *>446$) 6$) F) M`|dk `|dks spjni pjni \nks`j \nks`j u`ropmud u`ropmud udenm k`ifniepif k`ifniepif bprh|`kde` bprh|`kde` eni gderhmjhrhudnqden gderhmjhrhudnqden mnr`in udenm k`kdjdmd idjnd _b tnif |nkn e`ifni |unienr bprh|`kde` eni gderhmjhrhudnqden)

ENBUN_ SP\UNMN

Nihidk)>440) Bnrknmhs` Diehi`|dn*`ed|d DR) Anmnrun 1 E`snru`k`i M`|`gnuni _D Nihidk ) ?334 ) MJU) guus1((fr``ignud)ojhf|shu)lhk(?334(3>(mrhknuhfrnbd+jnsd|+udsd|)gukj ednm|`| uniffnj 35 K`d ?3>? Fnieanr* Doip Ughjdo eni Noepj _hgkni) ?336) Mdkdn Bnrkn|d Ninjd|d|) Thftnmnrun 1 PFK Mghsmnr*\)K)>443)Mhi|`s En|nr Mdkdn Ninjdudm)Anmnrun1PD+Sr`|| Khlg* Neini) >476) U`midm mrhknuhfrnbd piupm ninjd|d| ongni knmnini) Thftnmnrun 1 NIED