Advantages of Fed-batch Fermentation Over Batch Fermentation For

Advantages of fed-batch fermentation fermentation over batch fermentation for the production of PHA (polyhydroxyalk (polyhydroxyalkanoates) anoates)

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Higher PHA productivity •

Fed Batch Cultivation: –

–

Production of highest possible amount of PHA by balanced cell growth and PHA accumulation. Strategies: •

i)

Control of nitrogen limitation: No nitr nitrog ogen en limi limita tati tion on duri during ng the the act activ ive e gr growth owth phase, but it is introduced later to enhance PHA



Higher PHA productivity productivity cont. •

Batch cultivation cultivation is not an ideals method for PHA production especially for organisms organisms that cannot grow in nitrogen limitting condition. –

–

PHA accumulate during initiation of nitrogen limitation. Some organisms (eg: Pseudomona oleovorans) loses its viability after the onset of o f nitrogen



Limits the production of by-products •

By feeding the growth limiting substrate at the same rate as the substrate utilization by the organisms. –

–

Avoid the production of by-product that related to the presence of substrate concentration. Thus, increase the efficacy of carbon flux towards



Limits the production of by-products cont. •

In fed-batch: –

–

•

Growth limitation substrate (eg: nitrogen) can be control. Thus, production of by products can be control.

In batch: –

Growth limitationsubstrate (eg: nitrogen) can not be control. Therefore, the high concentration of substrate can

Advantages of Fed-batch Fermentation Over Batch Fermentation For

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