Verification Procedure for Accuracy and Precision

Standard Operating Procedure for Pipettes

Verication Procedure for Accuracy and Precision (dened or Users to incorporate in SOPs) In accordance with ISO8655 Standard

Procedure LT802292/F - © 2007 Gilson SAS All rights reserved

August 2007

Contents

Glossary

3

Introduction

4

Environmental Conditions

5

Technician Pipette Operation

6

Training

Pipette Tips

7 Test Equipment Test

Balance

8

Thermometer, Hygrometer and Barometer Thermometer,

8

Weighing Containers

9

Water

9

Verication Procedure

10

Procedure Summary

10

Multichannel Pipettes

10

Test Volumes

Estimating the Evaporation Rate (Mass Loss/Cycle)

12

Gravimetric Gravimetr ic Test

13

Calculations Formulae

14

Z-actor

15

Verication Procedure Report

16

Appendix

18

Associated Documents

22

Notes

23

In this document the word “tip” is used in the generic sense, where tip is the disposable part that must be used with the pipette: or Distriman this means “DistriTip”, or Microman “capillary-piston” and or Pipetman “Gilson Diamond Tip”. Page 

Glossary

adjustment

manuacture o an apparatus within appropriate tolerances, or the supplier’s setting o the apparatus, ensuring the metrological perormance, as specied in the applicable part o ISO 8655.

calibration

set o operations that establish the relationship between the dispensed volume and the corresponding nominal or selected volume o the apparatus.

maximum permissible error

upper or lower permitted extreme value or the deviation o the dispensed volume rom the nominal volume or selected volume o a piston-operated volumetric apparatus.

systematic error

dierence between the dispensed volume and the nominal volume or selected volume o the piston-operated volumetric apparatus.

random error

scatter o the dispensed volumes around the mean o the dispensed volumes.

uncertainty o measurement

parameter, associated with the dispensed volume, that characterizes the dispersion o the volumes that could reasonably be attributed to the dispensed volume.

nominal volume

volume specied by the manuacturer and used or identiication and or indication o the measuring range.

Page 

Introduction

This document describes a verication procedure or the ollowing Gilson pipettes: Pipetman® Ultra (Single and Multichannel), Pipetman® Concept (Single and Multichannel), Pipetman® P, Pipetman® Neo, Pipetman® F, Pipetman® 8X200, Microman® and Distriman®. The procedure is or veriying pipette perormance using gravimetric tests o repeated aspirate and dispense cycles with distilled water (grade 3, ISO 3696), in controlled conditions. The test conditions and methods described herein are ully compatible with ISO 8655 and are oten stricter than those specied in the international standard, as are the expected results or maximum permissible errors, which are tabulated in the Appendix. So, adherence to this procedure assures conormity to Gilson’s specications or accuracy (systematic error) and precision (random error) and to ISO requirements. The procedure, which or small volumes includes a correction or evaporation loss, evaluates the total system o pipetting: pipette, tip, and operator. Thereore the procedure must be carried out by suitably qualied and trained technicians. In calculating the volumes rom balance readings, corrections are made or the temperature and air pressure when the test was made (Z-actor, reer to page 15). Although the document does not directly concern itsel with other tests perormed by the user, the method and calculations described herein may be applied in other tests, outside the scope o this document. Users shall establish a regular testing routine at least once a year or their piston pipettes according to: accuracy and precision requirements, requency o use, number o operators using the pipette, number o operations on each occasion o use and the nature o the liquids being dispensed. In the case o Pipetman Ultra and Pipetman Concept, the number o cycles can be the basis o your regular testing routine.

Page 

Environmental Conditions

The test shall be carried out in a draught-ree room with a stable environment. The test room (laboratory) shall have humidity and temperature control so that the atmospheric conditions o the environment where the procedure will take place and the temperature o the equipment used are stable and homogeneous beore and during the procedure. The use o a chart recorder is recommended. The temperature o the pipettes being veried and the distilled water (grade 3, ISO 3696) used in the gravimetric test should have stabilized beore the procedure commences. The pipettes, water and test apparatus should have been placed in the test room at least 2 hours beore starting the tests. Ideally, verication takes place under the ollowing conditions: 1) Temperature (t)

ISO 8655 recommends that the gravimetric tests take place where the ambient and water temperature (t) are stable (± 0.5 °C) between 15 °C and 30 °C. Gilson recommends a range between 20 °C and 23 °C with a constant temperature (± 0.5 °C) between the beginning and the end o gravimetric tests. It is recommended to put water and pipettes at least 2 hours in the calibration room to reach an equilibrium with the room conditions. Z-actor is used to convert mass into volume according to temperature and pressure. 2) Relative humidity (RH)

ISO 8655 states that the RH must be greater than 50%. However, Gilson recommends that a humidity range o between 50% and 75% be maintained throughout the verication procedure. In all cases, the evaporation rate will be evaluated or volumes 50 µL. 3) Barometric pressure

Gilson’s tests should take place at 1013 ± 25 hPa. The barometric pressure in the test room shall be recorded to the nearest 0.5 kPa. Z-actor is used to convert mass into volume according to temperature and pressure.

Page 

Technician

Pipette Operation Consistency o pipetting technique contributes signicantly to the reproducibility o the results o the Verication Procedure. Inexperienced technicians can cause substantial variations in apparent pipette perormance. For meaningul test results, the technicians must be well-trained and qualied. You should operate the pipette according to the instructions given in the user’s guide o the pipette under test. Attention should be given to maintain a steady rhythm when aspirating and dispensing samples, speed and smoothness when pressing and releasing the push-button, and tip immersion depth. The test cycle time shall be kept to a minimum. It should not exceed 60 s.

Training The Verication Procedure described in this document must be carried out by a suitably qualied technician. We strongly recommend that the technician succeully completes a suitable Gilson training program. Please contact your local Gilson distributor or details.

Page 

Pipette Tips

In accordance with the instructions given in its user’s guide, the pipette under test must be clean (reer to the decontamination procedure), correctly assembled (reer to the user’s guide), and tted with a new Gilson tip beore starting the Verication Procedure. Because the quality o the tip used is a signicant actor in ensuring that a pipette perorms to specications, tip selection is specially important in verication procedure. For example, all models o Pipetman are calibrated at the actor y using Gilson Diamond tips, which are o the highest quality. Thereore, or Pipetman, you must only use the Gilson Diamond tips when carrying out the gravimetric test to have the best perormance and results.

Pipette model

Volume range

P, PN, U P10, P10N, U10 C10, C8x10, C1x10

0. µL to  µL 1.0 µL to 10 µL 0. to 10 µL

P0, P0N, U0 U8x0, U1x0

*: Not valid with flter tips D200.

 µL to 0 µL 1 µL to 0 µL

Tips

Filter Tips

D10, DL10 DF10, DFL10

DL10

DF0

P100N P100, U100, C100 C8x100, C1x100

10 µL to 100 µL 0 µL to 100 µL  µL to 100 µL  µL to 100 µL

P00 F to F00* P00N, U00 P8x00

0 µL to 00 µL  µL to 00 µL 0 µL to 00 µL 0 µL to 00 µL

U8x00, U1x00 C00 C8x00, C1x00

0 µL to 00 µL

D00

DF00

P1000N P1000, U1000 F0 to F1000

100 µL to 1000 µL 00 µL to 1000 µL 0 µL to 1000 µL

D1000

DF1000

C100 C8x100, C1x100

100 µL to 100 µL

D100

DF100

P000, U000 F000 C000

1 mL to  mL  mL 0. mL to  mL

D000

P10ml, U10ml, C10ml

1 mL to 10 mL

D10ml

DF100 D00

DF00

Page 

Test Equipment

To ensure the integrity o the Verication Procedure, all o the measuring instruments: balances, hygrometer and thermometers should be checked regularly.

Balance Inormation on suitable balances (some o which have more than one sensitivity range) is available rom the International Organization o Legal Metrology (OIML). Appropriate balances, conorming to OIML R76-1, should be used. Balances should be serviced, calibrated and certied by qualied technicians using weights traceable to an internationally recognized authority (OIML). Nominal Volume (µL)

Display (mg)

Balance Sensitivity

< 100 100 µL to 1000 > 1000

0.001 0.01 0.1

10 -g 10 -g 10-g

Note: These requirements are more rigorous than those specifed in ISO 8655-6, Table 1.

The sensitivity o the balance chosen must be consistent with the accuracy required, which is one tenth o the deviation to be assessed. For Pipetman (all models) and Microman, select the sensitivity according to the pipette’s nominal volume (see table). For Distriman, test volumes are specied or each DistriTip model according to specic aliquot volumes (choose the sensitivity accordingly).

The table on which the balance is placed must be equipped with a marble surace plate that is independent o the perimeter, to avoid transmitting vibrations. For the same reason, the table must not be in contact with a wall. Avoid placing the balance near to a window or near to a door to avoid too long a response time or the balance and irregular evaporation caused by drats or greenhouse eects.

Thermometer, Hygrometer, and Barometer Use a calibrated thermometer to measure the water temperature at the beginning and at the end o each test series. Use a thermometer with a maximum uncertainty o measurement o 0.2 °C. The hygrometer shall have a standard maximum uncertainty o 10% and the barometer a standard uncertainty o less than or equal to 0.5 kPa.

Page 8

Test Equipment

Lid

Lid

Pot B Pot A

Ref: F10018 Ref: 0100 Pot C

Weighing Containers Special containers are used to receive water rom the test pipette during weighing. Controlling evaporation during the gravimetric test is essential. To minimize evaporation, Gilson uses custom-designed cylindrical at-bottomed weighing containers made o nonporous plastic. A Weighing Kit (re: F144700) consisting o our sizes o container (Pot A, B, C, and D), lters (re: F123854) or cleaning them and tweezers (re: F144706), is available rom Gilson. Individual components o the kit are available as spares. When the volumes to be tested are less than 200 µL, Gilson uses weighing containers equipped with lids (see below). Pot A container and lid, both o which must be manipulated using tweezers to avoid handwarming, or volumes up to 20 µL. Pot B container and lid or volumes rom 20 to 200 µL. This container is tted with P 5000 O-rings to avoid hand-warming.

Ref: F1000

Pot C container (50 mm x 35 mm) or volumes rom 200 to 5000 µL.

Pot D

Pot D container (70 mm x 50 mm) or volumes greater than 5000 µL.

Water The liquid used or testing must be distilled or deionized water grade 3 (degassed) conorming to ISO3696 at room temperature. To avoid uctuations in water temperature, use a large container as a water reservoir (Pot C or Pot D). The reservoir should contain sufcient water or all o the tests.

Ref: F10

Page 


Procedure Summary The Verication Procedure certies both pipette accuracy and precision. Environmental conditions, test equipment, and other qualications previously described in this document should be implemented to assure the validity o the test results. Ater pre-rinsing the tip, record ten individual weighings per selected volume. For variable volume pipettes, three volume settings are selected per pipette model based on the pipette’s useul volume range (nominal, approximately 50 % and minimum volume or 10 % o the nominal volume). For xed volume pipettes (Pipetman F) only the nominal volume is used. 1 Set the pipette to its test volume (see table opposite). 2 Estimate the evaporation loss (or small volumes). 3 Perorm the gravimetric test: record the weighings on the Verication Procedure Report. 4 Perorm the calculations: record the results on the Verication Procedure Report. 5 Compare the results with the accuracy and precision specications given in the user’s guide o the test pipette.

Multichannel Pipettes

Note: Gilson recommends simpliying the process by ftting a tip to the tested channel, only.

Page 10

According to ISO 8655-6, “For the purpose o the test, each channel shall be regarded as a single channel and reported as such” . Consequently, or each o the ollowing procedures: ll all channels simultaneously when aspirating the test liquid, then expel only the test liquid aspirated by the channel being tested into the weighing vessel.


Test Volumes

Minimum Volume (µL)

Mid Range Volume (µL)

Nominal Volume (µL)

0. 0. 1  10 0 0 0 100 00 1000 1000

1 1  10 0 0 100 100 00 00 00 000

  10 0 100 100 00 00 1000 1000 000 10000

1  0 10 0 100

 10 0 1 00

10  0 100 0 1000

 0 00

 0 00

10 100 1000

0

100

00

  0 0

10 10 10 10

0 0 00 00

1 10 0 10 00 1000

 0 10 00 00 000

10 100 00 100 000 10000

 0 10

10 100 00

Pipetman PN P, U P10, U10, P10N P0, U0, P0N P100N P100, U100 P00N P00, U00 P1000N P1000, U1000 P000, U000 P10ml, U10ml Microman M10 M M0 M100 M0 M1000 Distriman DistriTip Micro DistriTip Mini DistriTip Maxi Pipetman Multichannel 8X00

Pipetman Ultra Multichannel 8x0 1x0 8x00 1x00 Pipetman Concept C10 C100 C00 C100 C000 C10ml

Pipetman Concept Multichannel C8x10, C1x10 C8x100, C1x100 C8x00, C1x00

1 10 0

Page 11


Estimating the Evaporation Rate (Mass Loss/Cycle) Weighing requires special care, or small volumes (< 50 µL, according to ISO 8655) use tweezers and weighing containers tted with lids (Pot A). The goal is to minimize, control and quantiy evaporation loss during the weighing cycle. Apart rom the design o the weighing vessel, the test cycle time is important. Evaporation is estimated by perorming a series o our simulated weighings, repeating the weighing cycle without dispensing to the weighing container. The total diference attributable to evaporation is calculated and divided by 4 to obtain an average. The rate is expressed in mg/cycle (or or one cycle the loss may be expressed in mg). For example, evaporation rates usually range or Pot A between 0.010 mg to 0.025 mg per weighing cycle. Recalculate the evaporation rate every 4 hours or whenever ambient conditions change (temperature, pressure, and humidity). 1 Add water to the weighing container until it is about one-third ull. 2 Fit the weighing container with its lid and use tweezers to place it on the balance pan. 3 Using the pipette, aspirate a sample rom the reservoir at the test volume setting. 4 Tare the balance and remove the weighing container rom the balance pan. 5 Use tweezers to remove the lid. 6 Dispense the sample into the reservoir or to waste, not the weighing container.

7 Fit the weighing container with its lid and use tweezers to put it back on the balance pan. 8 Record the result e1. 9 Repeat steps 3 through 8 three times to obtain e2, e3, and e4. 10 Calculate the loss/cycle: e = | e1 + e2 + e3 + e4 | /4 (mg). 11 The evaporation loss/cycle e (mg) should be added to the mean mass beore calculating the mean volume.

Page 1


Gravimetric Test Note *: ISO recommends that the oriice o the tip be immersed to between 2 mm and 3 mm below the surace o the water. However, you should frst consult the user’s guide or the model o Gilson pipette that you are testing.

According to ISO 8655-6: “The test shall be carried out in a draught-free room with stable environment.” 1

Place distilled or deionized water rom the container in the weighing vessel to a depth o at least 3 mm.* (Ret lid or Pot A and B.)

2

Record the test conditions (ambient and water temperature, relative humidity, barometric pressure).

3

Select the test volume o your variable-volume piston pipette.

4

Fit the tip or capillary/piston assembly to the pipette (the manuacturer specications are valid only when test executed with the manuacturers tips).

5

Wet pipette tip ve times to reach equilibrium in the dead air volume (not needed or Distriman and Microman), but do not take into account or calculations.

One test cycle should take less than 1 min. A consistent rhythm during weighing operation should be maintained.

Repeat these steps

6

Change tip.

7

Pre-wet the tip once.

8

Pipette the test volume.

9

Determine tare mass (reset balance).

10 Remove the lid i needed (using the tweezers or pot A) 11 Open balance door, retrieve weighing container, deliver sample, ret its lid, i needed, using the tweezers, replace on the balance and close the door. 12 Ater allowing display to stabilize and record the mass. 13 Repeat the test cycle until ten measurements have been recorded as a series o masses m1 to m10. 14 For sample below or equal to 50µl, estimate evaporation loss by repeating steps 8 to 10 exactly as a normal sample weighing but without actually adding any sample to the weighing container. Record absolute value (ei) and repeat several (m) times. 15 Record the test conditions (ambient temperature, relative humidity, barometric pressure). Check that values are still within recommended limits.

Page 1

Calculations

Formulae t = (t 1 + t 2 )/2

1 Calculate the mean temperature (t ) o the distilled water (rounded to the nearest 0.5 °C).

B = (B1 + B2 )/2

2 Use the average barometric pressure (B) and mean temperature (t ) to nd the corresponding Z-factor rom the table.

V i = Z (mi + e) V i = individual volumes (µL) mi = individual masses (mg) e = evaporation loss (mg)

3 Multiply the weighings (mg), ater any required correction or evaporation, by the Z-factor to obtain a series o volumes (µL).

Z = Z-factor (µL/mg) n

Σ V i

V =

i = 1

n

4 Compute the mean volume rom the series o volumes (µL).

V i = individual volumes V = mean volume n = number of weighings

5 Calculate the systematic error, which is the dierence between the mean volume o actual measurements and the true value as specied by the volume setting o the pipette (selected volume). For xed volume pipettes, replace V s with V o = nominal volume.

es = V - V s es = systematic error V = mean volume V s = selected volume

Accuracy may be expressed in µL or ... ... as a percentage.

es = 100 (V - V s )/V s %



S=

n

Σ (V - V)

2

i

i = 1

n-1

V i = individual volumes (calculated as above)

V = mean volume

6 Calculate the random error, which is the closeness o agreement between individual weighings. Quanties the magnitude o scatter due to random error. Also known as Repeatability Standard Deviation (RSD).

n = number of measurements s = repeatability standard deviation

CV = 100 x s/ V

Page 1

As a percentage, also known as coefcient o variation (CV ).

Calculations

Z-factor

Z= Conversion actor (µL/mg) t = Average temperature (°C) B = Air pressure (kPa)

Z correction actors or distilled water as a unction o test temperature and air pressure. 80

85

90

95 Z (µL/mg)

100

101.3

105

15.0

1.001

1.0018

1.001

1.001

1.000

1.000

1.000

15.5

1.0018

1.001

1.001

1.000

1.000

1.001

1.001

16.0

1.001

1.000

1.000

1.001

1.001

1.001

1.00

16.5

1.000

1.000

1.001

1.001

1.00

1.00

1.00

17.0

1.001

1.001

1.00

1.00

1.00

1.00

1.00

17.5

1.00

1.00

1.00

1.00

1.00

1.00

1.00

18.0

1.00

1.00

1.00

1.00

1.00

1.00

1.00

18.5

1.00

1.00

1.00

1.00

1.00

1.00

1.00

19.0

1.00

1.00

1.00

1.00

1.00

1.00

1.00

19.5

1.00

1.00

1.00

1.00

1.00

1.008

1.008

20.0

1.00

1.00

1.00

1.008

1.008

1.00

1.00

20.5

1.00

1.008

1.008

1.00

1.00

1.000

1.000

21.0

1.008

1.00

1.00

1.000

1.001

1.001

1.001

21.5

1.000

1.000

1.001

1.001

1.00

1.00

1.00

22.0

1.001

1.001

1.00

1.00

1.00

1.00

1.00

22.5

1.00

1.00

1.00

1.00

1.00

1.00

1.00

23.0

1.00

1.00

1.00

1.00

1.00

1.00

1.00

23.5

1.00

1.00

1.00

1.00

1.00

1.00

1.00

24.0

1.00

1.00

1.00

1.00

1.00

1.008

1.008

24.5

1.00

1.00

1.008

1.008

1.00

1.00

1.00

25.0

1.008

1.008

1.00

1.00

1.000

1.000

1.000

25.5

1.00

1.000

1.000

1.001

1.001

1.001

1.00

26.0

1.000

1.001

1.001

1.00

1.00

1.00

1.00

26.5

1.00

1.00

1.00

1.00

1.00

1.00

1.00

27.0

1.00

1.00

1.00

1.00

1.00

1.00

1.00

27.5

1.00

1.00

1.00

1.00

1.00

1.00

1.00

28.0

1.00

1.00

1.00

1.00

1.008

1.008

1.008

28.5

1.00

1.008

1.008

1.00

1.00

1.000

1.000

29.0

1.00

1.00

1.000

1.000

1.001

1.001

1.001

29.5

1.000

1.001

1.001

1.00

1.00

1.00

1.00

30.0

1.00

1.00

1.00

1.00

1.00

1.00

1.00

B

(kPa)

t (°C)

Page 1

Sample of a Verication Procedure Report

Pipetting System Information Pipette Serial number:

Calibration date:

Model:

Manufacturer:

Pipette owner: Number of channels:

Status:

Tips Tip model:

Manufacturer:

Batch number:

Environmental Factors Temperature air (°C):

Pressure (hPa):

Hygrometry (%):

Temperature water (°C):

Z-factor:

Evaporation (Yes/No):

General Information Decontamination (Yes/No):

Repair (Yes/No):

Adjustment (Yes/No):

Basis of adjustment (Ex/In):

Statistics Summary Selected Mean Volume Volume

n o i t n a o i i t r a r a V i r v  o e o r D t E c n i d e t r i a c a d f m n e t e a o s t y S : C : S D V : s S C E

Page 1

(µL)

(µL)

V min

V mean,min

V int

V mean,int

V nom

V mean,nom

Systematic error Results Target Status Es (µL)

Es (%)

µL

%

Random error Results Target SD (µL)

CV (%)

Status

SD (µL) CV (%)

or # l e n n a h C

Selected Mean Volume Volume

Systematic error Results Target Status

(µL)

(µL)

Es (µL)

1

V min1

V mean,min1

1

V int1

V mean,int1

1

V nom1

V mean,nom1

...

V min

V mean,min

...

V int

V mean,int

...

V nom

V mean,nom

N

V minN

V mean,minN

N

V intN

V mean,intN

N

V nomN

V mean,nomN

Es (%)

µL

%

Results SD (µL)

Random error Target

CV (%)

SD (µL) CV (%)

Status

Sample of a Verication Procedure Report

Calibration Details Selected Volume

Minimum Volume (value µL)

Mid Range Volume (value µL)

Nominal Volume (value µL)

1

Va1

Vb1

Vc1

Va10

Vb10

Vc10

Mid Range Volume (value µL)

Nominal Volume (value µL)

Va1,1

Vb1,1

Vc1,1

10

Va1,10

Vb1,10

Vc1,10

1

Vai,1

Vbi,1

Vci,1

10

Vai,10

Vbi,10

Vci,10

1

VaN,1

VbN,1

VcN,1

VaN,10

VbN,10

VcN,10

2 3 4 ... 10

or # . h C

1

Selected Minimum Volume Volume (value µL) 1 2 3 4 ...

i

2 3 4 ...

N

2 3 4 ... 10

Page 1

Appendix

Pipetman P / Neo

Here are comparative tables or maximum permissible errors between ISO 8655 and Gilson. ISO 8655 maximum permissible errors are very wide, so as to have a conormity-basis or all pipettes. At Gilson our knowledge and kno-how allows us to be more stringent, which means the best pipette-perormance.

Model (Reference)

Only or Pipetman Neo. Except or Pipetman Neo.

(µL)

Systematic Random error (µL) error (µL)


Max.

0. ± 0.0 0. ± 0.0  ± 0.00

 0.01  0.01  0.01

± 0.08 ± 0.08 ± 0.08

 0.0  0.0  0.0

P10 (F180) Min. P10N (F1) U10 (F10) Max

1 ± 0.0  ± 0.0 10 ± 0.100

 0.01  0.00  0.00

± 0.1 ± 0.1 ± 0.1

 0.08  0.08  0.08

P20 (F100) Min. P20N (F1) U20 (F10) Max.

  10 0

± 0.10 ± 0.10 ± 0.10 ± 0.0

 0.0  0.0  0.0  0.0

± 0.0 ± 0.0 ± 0.0 ± 0.0

 0.10  0.10  0.10  0.10

P100 (F11) Min. 10 P100N (F1) 0 U100 (F10) 0 Max. 100

± 0. ± 0. ± 0.0 ± 0.80

 0.10  0.10  0.1  0.1

± 0.80 ± 0.80 ± 0.80 ± 0.80

 0.0  0.0  0.0  0.0

P200 (F101) Min. 0 P200N (F1) 0 U200 (F10) 100 Max. 00

± 0.0 ± 0.0 ± 0.80 ± 1.0

 0.0  0.0  0.  0.0

± 1.0 ± 1.0 ± 1.0 ± 1.0

 0.0  0.0  0.0  0.0

P1000 (F10) Min. 100 00 P1000N (F1) U1000 (F10) 00 Max. 1000

± ± ± ±8

 0.  0.  1.0  1.

±8 ±8 ±8 ±8

 .0  .0  .0  .0

P5000 (F10) Min. 1000 and 000 U5000 (F10) Max. 000

± 1 ± 1 ± 0

  8

± 0 ± 0 ± 0

 1  1  1

P10ml (F1101) Min. 1 mL and  mL U10ml (F108)  mL Max.10 mL

± 0 ± 0 ± 0 ± 0

   10  1

± 0 ± 0 ± 0 ± 0

 0  0  0  0

P2 (F1801) P2N (F11) U2 (F101)

Pipetman Ultra

Maximum Permissible Errors Gilson ISO 8655

Volume

Min

Systematic error : expressed as the deviation o the mean o a tenold measurement rom the nominal or selected volume (see ISO 8655-6). Random error : expressed as the repeatability standard deviation o a tenold measurement (see ISO 8655-6 ).

Page 18

Appendix

Pipetman Concept Model (Reference)

C10 (F101)

Volume (µL)

Min.


0. 1  10

Max.

Maximum Permissible Errors Gilson ISO 8655 Systematic Random error (µL) error (µL)

± 0.00 ± 0.0 ± 0.00 ± 0.080

 0.01  0.01  0.00  0.0

± 0.10 ± 0.10 ± 0.10 ± 0.10

 0.080  0.080  0.080  0.080

C100 (F101)

Min.

 10 0 Max. 100

± 0. ± 0.0 ± 0.8 ± 0.

 0.10  0.10  0.1  0.1

± 0.8 ± 0.8 ± 0.8 ± 0.8

 0.0  0.0  0.0  0.0

C300 (F101)

Min.

± 0.80 ± 0.0 ± 0.0 ± 1.0

 0.1  0.0  0.  0.0

± .00 ± .00 ± .00 ± .00

 1.0  1.0  1.0  1.0

C1200 (F101) Min.

100 10 00 Max. 100

± . ± . ± . ± .0

 0.  0.  0.8  1.

± 1.0 ± 1.0 ± 1.0 ± 1.0

 .0  .0  .0  .0

C5000 (F101) Min. 00 00 000

± 10 ± 1 ± 

  

± 0 ± 0 ± 0

 1  1  1

C10ml (F101) Min. 1000 000 10000

±  ± 0 ± 0

 8  1

± 0 ± 0 ± 0

 0  0  0

0 0 10 Max. 00

Pipetman F Model (Reference)

Volume (µL)



F2 (F10)



± 0.08

 0.0

± 0.08

 0.0

F5 (F11)



± 0.10

 0.0

± 0.1

 0.0

F10 (F1)

10

± 0.10

 0.0

± 0.1

 0.08

F20 (F10)

0

± 0.0

 0.0

± 0.0

 0.10

F25 (F1)



± 0.

 0.0

± 0.0

 0.0

F50 (F18)

0

± 0.0

 0.1

± 0.0

 0.0

F100 (F18)

100

± 0.80

 0.

± 0.80

 0.0

F200 (F10)

00

± 1.0

 0.0

± 1.0

 0.0

F250 (F18)

0

± .00

 0.

± .00

 1.0

F300 (F188)

00

± .0

 0.

± .00

 1.0

F400 (F18)

00

± .0

 0.80

± .00

 1.0

F500 (F10)

00

± .00

 1.00

± .00

 1.0

F1000 (F10)

1000

± 8.00

 1.0

± 8.00

 .00

F5000 (F10)

000

± 0.00

 8.00

± 0.00

 1.00 Page 1

Appendix

Microman Model (Reference)


Volume (µL)


1  10

± 0.0 ± 0.10 ± 0.1

 0.0  0.0  0.0

± 0.0 ± 0.0 ± 0.0

 0.10  0.10  0.10

Max.

 10 

± 0. ± 0. ± 0.0

 0.08  0.08  0.10

± 0.0 ± 0.0 ± 0.0

 0.0  0.0  0.0

Min. Max.

0 0

± 0. ± 0.0

 0.0  0.0

± 0.0 ± 0.0

 0.0  0.0

Min.

10 M100 (F180) 0 Max. 100

± 0.0 ± 0. ± 1.00

 0.0  0.0  0.0

± 1.0 ± 1.0 ± 1.0

 0.0  0.0  0.0

Min.

± 1.0 ± 1.0 ± .0

 0.0  0.0  0.0

± .00 ± .00 ± .00

 .00  .00  .00

Min.

± .00 ± .00 ± 8.00

 1.0  .0  .00

± 1.00 ± 1.00 ± 1.00

 .00  .00  .00

Min. M10 (F1801) Max Min. M25 (F180)

M50 (F180)

0 M250 (F180) 100 Max. 0 100 M1000 (F180) 00 Max. 1000

Distriman DistriTips Model

Volume (µL)

(Reference)



 ± 0.100  ± 0.1 10 ± 0.00

 0.080  0.0  0.100

± 0.0 ± 0.0 ± 0.0

 0.10  0.10  0.10

1250 µL Min. 0 ± 0.80 Mini (F1110) 0 ± 1.00 Mini ST (F110) Max. 100 ± 1.00

 0.0  0.0  0.0

± 1.0 ± 1.0 ± 1.0

 0.0  0.0  0.0

12.5 mL Min. 00 ± .00 Maxi (F110) 00 ± .0 Maxi ST (F110) Max. 1000 ± 10.00

 1.00  1.0  .0

± 1.00 ± 1.00 ± 1.00

 .00  .00  .00

125 µL Min. Micro (F1100) Micro ST (F110) Max

ST means Sterilized.

Page 0


Appendix

Pipetman Ultra Multichannel

Model (Reference)

8x20 (F100) and 12x20 (F101)

Volume (µL)



1  10 0

± 0.10 ± 0.10 ± 0.0 ± 0.0

 0.08  0.08  0.10  0.0

± 0.0 ± 0.0 ± 0.0 ± 0.0

 0.0  0.0  0.0  0.0

0 0 10 Max. 00

± 1.00 ± 1.00 ± 1.0 ± .00

 0.  0.  0.0  1.00

±8 ±8 ±8 ±8

 .00  .00  .00  .00

Min.

Max 8x300 (F10) and 12x300 (F10)


Min.

Pipetman 8X200 Model (Reference)

8x200 (F1100)

Pipetman Concept Multichannel

Model (Reference)

Volume (µL)


Min.

0 0 100 Max. 00

Volume (µL)

± 0.0 ± 0.0 ± 1.00 ± .00

 0.  0.  0.0  1.00


± .0 ± .0 ± .0 ± .0

 1.0  1.0  1.0  1.0



1  10

± 0.0 ± 0.08 ± 0.10

 0.0  0.0  0.0

± 0. ± 0. ± 0.

 0.1  0.1  0.1

10 0 Max. 100

± 0. ± 0.0 ± 0.80

 0.1  0.0  0.

± 1.0 ± 1.0 ± 1.0

 0.0  0.0  0.0

C8x300 (F10) Min. 0 and 10 C12x300 (F10) 00

± 1.00 ± 1.0 ± .0

 0.18  0.8  0.0

± 8.00 ± 8.00 ± 8.00

 .00  .00  .00

C8x10 (F10) Min. and C12x10 (F10) Max. C8x100 (F10) and 12x100 (F10)

Min.

Page 1

Associated Documents

Documents

Page 

Gilson Reference

Pipetman Ultra User’s Guide

LT801441

Pipetman P User’s Guide Pipetman Neo Addendum

LT801117 LT801511

Pipetman F User’s Guide Pipetman 8X200 User’s Guide Pipetman Ultra Multichannel User’s Guide

LT801118 LT801236 LT801462

Pipetman Concept User’s Guide Microman User’s Guide Distriman User’s Guide

LT801489 LT801502 LT801285

Decontamination Procedure

LT802288

Notes

Page 

World Wide Web : www.gilson.com &: [email protected], [email protected], [email protected]

World Headquarters Gilson, Inc.

3000 Parmenter Street, P.O. Box 620027, Middleton, WI 53562-0027, USA Telephone: (1) 800-445-7661 or (1) 608-836-1551 • Fax: (1) 608-831-4451 Gilson SAS

19 avenue des Entrepreneurs, B.P. 145 95400 Villiers-le-Bel, France Telephone: (33) 1-34-29-50-00 • Fax: (33) 1-34-29-50-20

Verification Procedure for Accuracy and Precision

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