III B.Tec B.Tech h II Semest Semester er Supplim Supplimen entar tary y Examin Examinati ations ons,, Aug/S Aug/Sep ep 2007 2007 BIOPROCESS ENGINEERING-II (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆ ⋆ ⋆ ⋆ ⋆
1. What is meant by Multiphas Multiphasee bioreac bioreactors tors ? Explai Explain n in detail about any two Multiphase bioreactors ? [16] 2. Write short notes on:
[8+8]
(a) One-parameter One-parameter model model (b) Two-parameter Two-parameter model 3. Define non-mechani non-mechanically cally agitated agitated vessels? vessels? Explain Explain the design and operation of such a reactor? [16] 4. Write in detail about the methods employed in measuring the following process variables. [8+8] (a) Temperature (b) Pressure Pressure.. 5. (a) Discuss Discuss in detai detaill about about ON-Line ON-Line Sensor. Sensor. (b) Discuss in detail about OFF-Line Analytical Method odss
[8+8]
6. What do you mean by a vector and explain its properties.
[16]
7. Write Short Notes On:
[16]
(a) Monoclonal antibodies. antibodies. (b) Immunobiol Immunobiological ogical Regulators. Regulators. (c) Virus Vaccines. Vaccines. (d) Hormones. Hormones. 8. Discuss Discuss in detail about segregated kinetic kinetic models for growth and product formation. [16] ⋆ ⋆ ⋆ ⋆ ⋆
1 of 1
Set No. 2
Code No: RR3223 RR322304 04
III B.Tec B.Tech h II Semest Semester er Supplim Supplimen entar tary y Examin Examinati ations ons,, Aug/S Aug/Sep ep 2007 2007 BIOPROCESS ENGINEERING-II (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆ ⋆ ⋆ ⋆ ⋆
1. Define Fed Fed batch culture ? What are the different different strategies employed employed in Fed Fed batch systems and discuss about them ? [16] 2. Explain in detail ab out mo dels for Non-Ideal reactors ?
[16]
3. What is Cyclic fed batch culture ? What area the applications of fed-batch culture and examples of the use of fed batch culture? [16] 4. Write in detail about the methods employed in measuring the following process variables. [8+8] (a) Temperature (b) Pressure Pressure.. 5. (a) Discuss Discuss in detai detaill about about ON-Line ON-Line Sensor. Sensor. (b) Discuss in detail about OFF-Line Analytical Method odss
[8+8]
6. Explain the guide lines for choos oosing Host-Vector system.
[16]
7. Write Short Notes On:
[16]
(a) Monoclonal antibodies. antibodies. (b) Immunobiol Immunobiological ogical Regulators. Regulators. (c) Virus Vaccines. Vaccines. (d) Hormones. Hormones. 8. Discuss in detail about the factor which interact to determine pellet formation and pellet structure during cultivation of mycelial organism. [16] ⋆ ⋆ ⋆ ⋆ ⋆
1 of 1
Set No. 3
Code No: RR3223 RR322304 04
III B.Tec B.Tech h II Semest Semester er Supplim Supplimen entar tary y Examin Examinati ations ons,, Aug/S Aug/Sep ep 2007 2007 BIOPROCESS ENGINEERING-II (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆ ⋆ ⋆ ⋆ ⋆
1. Explain the basis for classification of Bioreactors and write in brief about them with an example? [16] 2. Explain in detail about the two different design approaches for continuous sterilization pro cess ? [16] 3. Write about the different configurations of Air-lift fermentor their design and operation? [16] 4. Explain ab out the On-line sensors for cell prop erties?
[16]
5. (a) Discuss Discuss in detai detaill about about ON-Line ON-Line Sensor. Sensor. (b) Discuss in detail about OFF-Line Analytical Method odss
[8+8]
6. Discuss in detail the control and information sequences in DNA guide the transcri script ptio ion n an and d tran transa saat atio ion n proc proces esss wh whic ich h resu result lt in gene gene expr expres essi sion on.. [16] [16] 7. Discuss in detail ab out pro duct formation kinetics.
[16]
8. Disc Discus usss in deta detail il ab abou outt gro growth wth cycl cyclee ph phas ases es for for ba batc tch h cult cultiv ivat atio ion. n.
[16] [16]
⋆ ⋆ ⋆ ⋆ ⋆
1 of 1
Set No. 4
Code No: RR3223 RR322304 04
III B.Tec B.Tech h II Semest Semester er Supplim Supplimen entar tary y Examin Examinati ations ons,, Aug/S Aug/Sep ep 2007 2007 BIOPROCESS ENGINEERING-II (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆ ⋆ ⋆ ⋆ ⋆
1. Define Define Batch Growth? Growth? Explain Explain about the growth growth patterns patterns and Kineti Kinetics cs in Batch Batch culture? [16] 2. Expl Explai ain n in deta detail il about about two-pa o-para rame mete terr mode modell for for Non Non idea ideall reac reacto tors rs??
[16] [16]
3. List out the cardinal rules to be followed in design of a fermentor and its construction materials for successful op eration? [16] 4. Explain the methods involved in measurement of process variables such as pH and dissolved oxygen? [16] 5. (a) Discuss Discuss in detai detaill about about ON-Line ON-Line Sensor. Sensor. (b) Discuss in detail about OFF-Line Analytical Method odss
[8+8]
6. Dis Discu cuss ss in deta detail il about about the the overvi erview ew of info inform rmat atio ion n flow flow in the the cell cell..
[16] [16]
7. What are the regulatory constraints on genetic pro cess?
[16]
8. Discu iscuss ss in deta detail il ab abou outt gro growth wth cycl cyclee ph phas ases es for for ba batc tch h cult cultiv ivat atio ion. n.
