Report-IBD-Visit-3.docx

GROUP 4

CHANG WEN QI | KHAIRUL KH AIRUL ANWAR ANWAR | SYAHIRA SYAELLA SYAELLA

CONTENTS

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ACKNOWLEDGEMENT OBJECTIVES INTRODUCTION EQUIPMENT AND AND INSTRUMENT IN IBD I BD : 1. LIQUID CHROMA CHROMAT TOGRAPHY OGRAPHY-- MASS SPECTROME SPECTROMETRY TRY (LC-MS) (LC-MS) 2. SOH SOHLE LET T ETR ETRAC ACTO TOR  R  !. ROTAR ROTARY Y EVA EVAPORAT PORATOR  ". SPRA SPRAY Y DRY DRYER  #. HIGH HIGH PRES PRESSUR SURE E LOOP LOOP REACT REACTOR  OR  $. MOLEC MOLECULA ULAR R DISTIL DISTILLA LAT TION %. HIGH HIGH PRES PRESSU SURE RE REAC REACTO TOR  R  &. BIOREACTOR  '. CENTRIUGE 1. TURBO ETRA ETRACTO CTOR  R  LABORATORIES LABORATORIES AND DEPA DE PARTMENTS RTMENTS VISIT VISI T 1. COSMECEUTI COSMECEUTICAL CAL * RAGRANC RAGRANCES ES DEP DEPARTM ARTMENT ENT 2. OOD * NUTRAC NUTRACEUTIC EUTICAL AL DEPAR DEPARTME TMENTS NTS !. INE INE CHEM CHEMIC ICAL AL PIL PILOT OT PLA PLANT NT CONCLUSION REERENCE

ACKNOWLEDGEMENT

+, /0 3 4+5 6/787 0,/9 A53 48 48,8 +;87  //< 585 / 87< 58 +7<=,+ ,+> / IBD3 UTM. A >86+ >>,86++/7 4 8?>,88< / /=, 86=,8, 45+65 ,8

M<9 N/, +@5 B+7+ R@+3 D, M@=@+5 B+7+ A59< 7< M<9 ,57 B+7+ M/5< H 0/, 4++7 / >87< 58+, +98 87<+7 58 ,+> 4+5 =. =,58,9/,83 48 4/=< / +8 / 8?>,8 /=, >86+ ,+=<8 /4,< 58 <+,86/,  /0 I7+=8 /0 B+/>,/<=6 D8;8/>987 /0 U7+;8,++ T87//+ M+3 P,/0 R97 A<= A@+@ 45/ ;8 = >8,9++/7 / ;++ 58 +7+=8. S>86+ 57 /8 / 58 00  +7;/;8< 45+65 48,8 M, M=599< S=,+ B+7 A. R5973 M, A9,7 B+7 B=7 3 M+  N=,= L+@ 3 M+ H+95 7< /58, 00 /0 I7+=8 /0 B+/>,/<=6 D8;8/>987 45/ ;8  / /0 =80= +70/,9+/7 / = /= 58 965+78 7< + =8. L = 7/ 83 48 4/=< +8 / 57 /=, 698 45/ +7;/;8< +7 5+ +7<=,+ ,+> / I7+=8 /0 B+/>,/<=6 D8;8/>987 3 U7+;8,++ T87//+ M+ 0/, 58+, =>>/,.

OBJECTIVES:

T58 >=,>/8 /0 5+ +7<=,+ ;++ +:

1. T/ +;8 8?>/=,8 0/, 58 =<87 / 7/4 58 8=+>987 7< +7,=987 =8 +7 +7<=, ,88< / C589+6 R86+/7 E7+788,+73 S8>,+/7 E7+788,+7 T8657// 7< /58, =86. 2. T/ 7/4 58 0=76+/73 />8,+7 6/7<++/7 7< >,/68<=,8 / =8  58 8=+>987 7< +7,=987. !. T/ >,/;+<8  88, =7<8,7<+7 /0 58 58/, 8,78< +7 6 4+5 >,6+6 8,7+7 7< F57<-/7F +7 58 +7<=,. ". T/ +76,88 58 8;8 /0 7/48<8 7< +78,8 +7 58 FR88,65 7< D8;8/>987F C589+6 E7+788,+7 T8657// 7< B+/>,/<=6 <8;8/>987.

INTRODUCTION:

I7+=8 /0 B+/>,/<=6 D8;8/>987 (IBD) +  C87,8 /0 E?688768 +7 U7+;8,+ T87//+ M+ (UTM) 0/6=8< /7 R88,653 D8;8/>987 7< C/998,6++@+/7 /0 +77/;+;8  +/>,/<=6. I =>>/, +7 <8;8/>+7 5+5 ;=8 6/9>/787 7< >,/68+7 8657// 0/,  +7<=,+8 =65  7=,68=+63 6/9868=+63 >5/6589+63 0;/=, 7< 0,,7683 0//< +7,8<+873 >,/+/+63 +/08,++@8, 7< +/>8+6+<8. IBD + 8=+>>8< 4+5 9=+ >=,>/8 06+++8 7< 57<8<   ,/=> /0 5+5 +8< 9=+-<+6+>+7, 8?>8,+8768<  >8,/778. R88,65 + 6,,+8< /= +7 58 /,/,+8 5 988 58 +78,7+/7 7<,< 8;8 7< 65+8;8 ISO 66,8<++/7. IBD / 8,;8 + 6=/98, / <8;8/> +/>,/<=6 5,/=5  6/9>88 >,/68. IBD 5 887 /00+6+8<  58 H/7/,8 P,+98 M+7+8,3 D/ S8,+ A<=5 +7 H+ A59< B<4+ /7 $ J=78 2#. 

T58 V++/7: T/ 8 7 +77/;+;8 8<8, +7 +/>,/<=6 ,88,65 7< <8;8/>987



T58 M++/7: T/ <8;8/> +77/;+;8 9,8 <,+;87 +/>,/<=6 / >,/9/8 7< 875768 4878 7< 585 +;+7 0,/9 7=, ,8/=,68 5,/=5 =+78 >,/68 7< 6,8+7 485 0/, 58 7+/7.

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R88,65 T5898: B+/8657// 0/, W878 I7<=,.



O86+;8 /0 IBD

T/ 6,, /= R88,65 * B=+78 D8;8/>987 /0 +/>,/<=6 5,/=5 +77/;+;8  >,/688 :•

/6=+7 /7 5+5 87< 0=7<987 ,88,65 4+5 7/;8 7< +9>60=

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 >=+6+/7. P,/;+<+7 7< <8+7+7 9,8 <,+;87 +/>,/<=6 5 8780+ /6+8 7<

•

86/7/9. O008,+7 6/7=76 * 6/7,6 ,88,65 8,;+68 5,/=5 =++@+/7 /0 /=,  T8657//+6

•

P0/,9

B+/>,/68+73

H8,

*

P/6589+6

7<

B+/;+<+/7. U++@+7 89+ +7<=,+ 68 >,/68 8=+>987 4+5 08?+++ =>>/, 0/,  P,/68 * P,/<=6 D8;8/>9873 P,/68 S6+7 U>3 P7 O>+9+@+/7 * T,/=85//+7.

T/ >,/<=68 5+5 =+0+8< 5=97 6>+ 5,/=5 L+08 L/7 L8,7+7  :•

P,/;+<+7 7 8?87+;8 ,+7+7 +7 58 8;8 /0 P,/08+/7 S5/, C/=,8 * C8,+0+68 L8;8 P,/,998 +7 58 6/>8 /0 B+/8657//3 H8, I7<=,3

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H/++6 T58,> * W878 I7<=,. O,7+@+7 6/=,8 ,88< / P,/68 * P7 T8657// 0/, E7+788, 7< P7

•

O>8,/, +7 7 +7-5/=8 9/<8 65898. O008,+7 >/,<=8 >,/,998 +7 58 8;8 /0 M8,

* P5D 0/, 

 +/8657// 7< >7 8657// 86/,.

EQUIPMENT AND INSTRUMENT IN IBD 1.

LIQUID CHROMATOGRAPHY- MASS SPECTROMETRY (LC-MS)

LC-MS ,808, / 58 6/9+7+/7 /0 +=+< 65,/9/,>5+6 8>,+/7 (/, HPLC) 4+5 9 >86,/98,+6 <886+/7 (MS). L+=+< 65,/9/,>5 878, =++@8

;8, 9 >,+68 >68< 7< />8,8<  5+5 >,8=,83 ,808,,8< / 5+5  >8,0/,9768 +=+< 65,/9/,>5 (HPLC). M/<8,7 LC-MS 985/< =8< HPLC +7,=987+/7 / +7,/<=68 9>8. I7 HPLC 58 9>8 + 0/,68<  +=+<  5+5  >,8=,83 7/47  9/+8 >583 5,/=5  6/=97 5 + >68< 4+5 +/7,  >58 / 66/9>+5 >,+6=, >8 /0 8>,+/7. A 0/, 58 9 >86,/988,3 + +  8657+=8 / 98=,8 58 9-/-65,8 ,+/ /0 65,8< >,+68. T58 4/,  +/7+@+7 6589+6 6/9>/=7< / 878,8 9/86=8 0,987 7< 98=,+7 58+,  9-/-65,8 ,+/. 

I7 MS >,/68<=,8:  A 9>8 4 +7,/<=68< +7/ MS +7,=987 7< =7<8,/8 ;>/,+@+/7.  T58 6/9>/787 /0 9>8 ,8 +/7+@8< 7< 0/,98< 65,8< >,+68.  T58 +/7 ,8 8>,8< 66/,<+7 / 58+, 9-/-65,8 ,+/  886,/978+6 0+8<.  T58 +/7 ,8 <8868< 7< >,/688< +7/ 9 >86,. T58 MS 8657+=8 5 /5 =7++;8 7< =++;8 =8. T58 =8 /0 LC-MS 5  887 4+<8 <8;8/>8< +7 >5,96/+78+6 =<+8 /0 >5,968=+6 7< 5= 58 9/ 0,8=87 =8< 8657+=8 +7 58 0+8< /0 +/7+. I7 IBD3 58 ,88,658, 5;8 >,/0+8< +>+< +7 6/6/7= /+ =+7 =,-5+5  >8,0/,9768 LC-MS. T58 +>+< >,/0+8 /0 5,88 <+008,87 8657+=8 /0 >,/688 =65  +78,8< >,/683 48 >,/68 (08,987+/7) 7< <, >,/68 (6/< >,/68) 5;8  887 <8;8/>8< 7< 6/9>,8< =+7  /04,8. T58 =8 /0 LC-MS 5;8 6/7,+=8< / 6/9868=+6 7< 0,,768 0+8< 8>86+ +7 >,/<=6+7 5+, 83 5+, 6,893 +7 7=,+87 6,89 7< +> 9. A66/,<+7 / 98, =<87  IBD3 LC-MS 4 =8< / 8?,6 8+6 6+< 0,/9 ,<++/7 >7 (8+9+7 < 7< 6+> 0+95)  58 6+< 67 /> 58 ,/45 /0 6768, 68.

. SO!HLET E!TRACTOR 

A /?58 8?,6/, +  /,/, >>,= 0/, 58 8?,6+/7 /0 +>+< 7< /58,  9/86=8 0,/9  /+< 9>8. A /?58 8?,6+/7 >>,= + 6/9>/8< /0  6/7<878,3  /?58 8?,6/,3 7< ,/=7< //98< 0. I +  65 >,/68 7<

/+<-+=+< 8?,6+/7 >,/68. W587 /+< 4 +7,/<=68< +7/ 58 965+783 58 /=8 4 8?,6 0,/9 58 /+<. A08, 53 58 /=8 /8 5,/=5 ,/, 8;>/,/,  / /+7 /=, <8+,8< >,/<=6.



P,+76+>8 /0 S/?58 E?,6/,: A /?58 8?,6+/7 985/< + /9845 />>/+8 0,/9 0+,+/7 +7 5 58 8?,6+/7 /0 58 9>8 + 0/, 7 +7/=8 /,7+6 6/9>/787 +7  /;873 =7+8 6/9>88 /=++ /0 7 /,7+6 6/9>/787 +7  9>83 45+65 + =+8 0/, = +9>8 0+,+/7. T58,80/,83  /?58 8?,6+/7 >,/68<=,8 /4  6/9>/=7< / 8 8?,68< 0,/9 +7/=8 98,+ =+7  6589+6 /;87.



A>>+6+/7 /0 S/?58 E?,6/,   //< 8+7  B+/0=8  E7;+,/7987 7+ /0 /+3 =<83 7< 48.

". ROTARY EVAPORATOR 

T58 R/, E;>/,/, + =8< 9+7 0/, 58 6/7+7=/= <+++/7 =7<8, ,8<=6+7  >,8=,8 58 6/7<++/7 8?657+7 58 ;/+8 /;87 +7  ,8 9/=7. E>86+ / 58 <+++/7 /0 ,868+;+7 58 +=+<  58 +98 /0 6/7687,+/7 7<

65,/9/,>5+6 0,6+/7+/7 /0 =8/= 8?,63  ,+5 8>,+/7 7< >=,+0+8< ,86+/7 >,/<=6. I7 5+ +7,=9873 58 >,/68 +7;/;8< + +=+<-+=+< 8?,6+/73 458,8 48 47 / 8?,6 /=, <8+,8< >,/<=6.

T58 R/, E;>/,/, 98 58 0 =7<8, 8+7 9/ =+8 0/, 58 >88< 5,/=5 886,/7+6 6/7,/ 58 6/77 ;8/6+ + ,/8< +7 /,<8, / +76,88 58 8;>/,+/7 ,8. E78 58 8;>/,+/7 0 =7<8, 78+;8 >,8=,8 5,/=5 58 ;6==9 >=9>. E;>/,8 03 ,/83 >= +7/ 48, 5 >7  6/77 89>8,=,8 58+7  58 98 +983 /=+/7 5/=<8,3 <8>,8 +7 ,/+7 0 /+7 /73 58+73 >,8<+73 7< 8;>/,+7 +7 58 /8. T58 8;>/,/, 89 /0 58 ,/+/7 67 8 7< ,8<=68 >,8=,8 / "- $/,, H8 58 /;87 +7 58 <+++/7 0  58+7 58 53 58 58+7 89>8,=,8 67 8 6/8 / 58 /++7 >/+7 /0 5+ /;87. I7 <<++/73 =7<8, 5+5-800+6+87 6//8, 0=76+/73 67 +=80 58 5/ ;>/=,  ,>+<3 6688,8 58 8;>/,+/7 ,8. I7 IBD3 58 965+78 4 =8< / 8?,6 887+ /+. E87+ /+ 4 8?,68< 0,/9 ;,+/= 58, /+7+7  ,/=7< M+ =65  6 >>8,3 6+> 0+953 +78, /+3 97/887 7< 97 9/,8. T58 /+ 4 8?,68< 7< 97=06=,8< / 0/,9 ;,+8 /0 =80= >,/<=6 =65  0/,  98<+6 >=,>/8 7< 6/98+6 >=,>/8. #. SPRAY DRYER  S>, <,+7 +  985/< /0 >,/<=6+7  <, >/4<8, 0,/9  +=+< /, =,,  ,>+<

<,+7 4+5  5/ . T5+ + 58 >,808,,8< 985/< /0 <,+7 /0 97 58,987++;8 98,+ =65  0//< 7< >5,968=+6

C>6+: - ! +,85,  - # +,85,  - 1 +,85,  O>8,+7 T89>8,=,8: -

! / "# /C

+,3 58 +=+< 088< + >=9>8< 5,/=5 7 /9+8, <8;+68 5 >,/<=68 0+78 <,/>8 +7/ 58 9+7 <,+7 6598,. T58 >, <,8, + 8=+>>8< 4+5 4/ >8 /9+8,3 4/ 0=+< 7/@@8 7< ,/, /9+@8,.

B =+7 4/ 0=+< 7/@@83

6/9>,88< +, + =8< / 58> /9+@+7 458,8  +7 ,/, /9+@8, 58 088< + /9+@8< 4+5 58 58> /0 >+77+7 4588 45+65 ,/8 => / !3 ,>9. T587 58 5/ <,+7  67 8 >8< 5,/=5 58 6598, 988 58 0+78 08< <,/>8. T58 48,  0,/9 58 <,/>8 4+ 8;>/,8 7< 8;+7 58 /+< 6/787 +7 58 0/,9 /0 >/4<8,. T58 >/4<8, 7< 48, ;>/=, 4+ 587 / /  66/78 8>,/, +7 45+65 58 >/4<8,  4+ 8 8>,8< 0,/9 58 48, ;>/=, 7< 5/ +,. S>, <,+7 + 58 9/ 4+<8 =8< +7<=,+ >,/68 +7;/;+7 >,+68 0/,9+/7 7< <,+7. T58 >, <,8,  IBD + 8?87+;8 =8< / >,/68 58, 8?,6. A9/  M+7 >/>=, 58, 5;8 887 >,/688< 4+5 =668. T58 >, <,+7 />8,+/7  IBD + /68< +7 687 ,//9 ,8. T58 >,/68<=,8 7< +7+/7 988 58 ,8=+,8987 /0 GMP. W8 67 87=,8 5 58 >,/<=6 >,/68 + 687 7< 5+87+6. $. HIGH PRESSURE LOOP REACTOR  H+5 P,8=,8 L//> R86/, 67 />8,8 8+58, +7 6/7+7=/= 9/<8 /, 65 9/<8. I7  65 9/<8 58 >7 6>6+ + & 65 458,8  +7 6/7+7=/= 9/<8 58 6>6+ + => / ! 5,.

O>8,+7 M/<8: - B65 - C/7+7=/= C>6+: - B65: &65 - C/7+7=/=: 2 / ! +,85,  O>8,+7 T89>8,=,8: -

U> / 2# /C

O>8,+7 P,8=,8: -

U> / " , 

T5+ 5+5 >,8=,8 //> ,86/, >,/;+<8 ;8, ,+/,/= 9+?+7 0/, ,86+/7 8>86+ 0/, +=+<- 9+?+7  48  +=+<-+=+< 9+?+7. D=,+7 58 ,86+/7  ,8 +7,/<=68< ;+ ,86+/7 9+?8, / 6,88  48 <+>8,8< 9+?=,8 5 + +7868<  5+5 ;8/6+ +7/ 7 =/6;8. T5+ 9+?=,8 + ,>+< 6+,6=8<   >86+ >=9>  5+5 +=+< ,+/ 5,/=5/= 58 ;/=98 /0 58 //> 7< >8,9+ 58 9?+9=9 >/+8 9 ,708, ,8. A 58 8?6578, +7 58 8?8,7 //> /4 0/, +7<8>87<87 />+9++/7 /0 58 ,708,. /, 6/7+7=/= />8,+/73 >,/<=6 + 8>,8<  7 +7-+78 6,/-0/4 0+8, 45+65 ,8+7 58 =>87<8< /+< 6 4+5+7 58 //>. T58 >7 4 <8+7 7< =+ / 6/9> 4+5 8?>/+/7 >,//0 87;+,/7987 7< =+8 / 57<8. T58 ,78 /0   >,/68 ,86+/7 6,,+8< /= +7 588 >7 +76=<8: H<,/87+/73 A/?+/73 O?+<+/73 A9+7+/73 R8<=6+;8 A9+7+/73 A+/73 R8<=6+;8 A+/73 C,/7+/73 C5/,+7+/73 D85<,/87+/73 E5/?+/7  P,/>/?+/73  N+,+8 758+3 P5/87+/7 7< 97 9/,8. %. MOLECULAR DISTILLATION

C>6+: - !# 5,  O>8,+7 T89>8,=,8: -

21 /C

O>8,+7 P,8=,8: -

.1 9, 

 N=98, /0 8: -

!

M/86=, <+++/7 8>,8 ;/+8 0,/9 8 ;/+8  =++@+7 58 5+7-0+9 4+>+7 6+/7 /0 088< +=+< /7 +7+<8 4 /0  588< 6+7<,+6 ;6==9 6598,. A 58 ,8= 58 ;/+8 4+ 8;>/,8. T58 ;>/=, 4+ 8 6/7<878< +7 8>,8 6/7<878, 7< 58 +=+< 4+ 8 <,47  58; >,/<=6 /, /8 / 58 78? 8. T5+ >,/68 + 7 800+6+87 58,9 8>,+/7 8657+=8 4+5 9+7+9=9 >,/<=6 <86/9>/++/7 7< 9?+9=9 >,/<=6 =+. T58 <;78 /0 9/86=,  <+++/7 >,/68 +76=<8 5/, ,8+<8768 +98 /0 58 088< +=+<3  +7+0+67 /48,8< 89>8,=,8 <=8 / 5+5 ;6==9 6>++3 7< />+9 800+6+876 +7 9 7< 58 ,708,. T58,80/,83 5+ >7 + =+8 0/, >,/68+7 58 87++;8 /0  5+5 ;=8 >,/<=6. T58 +7<=,+8 0,/9 58 0//4+7 0+8< 4+ 8780+ 0,/9 5+ >7: P5,968=+63 B+/98,+ C/7687,+/7 • M/86=, D+++/7 O0 E8,3  A6+<3 M/7/3 D+3 * T,+68,+<8 • V+9+73 N=,68=+63 E87+ O+ I/+/7 • W? ,6+/7+/7 • O+ D8/>9 L8;8 • //<3 ;/, P=,+0+6+/7 • S/;87 R86/;8, • &. HIGH PRESSURE REACTOR  H+5 >,8=,8 +  +,,8< 7 ,86/, 8=+>>8< 4+5 +/,. T58 ,86/, 67 8 588< => / 2# C =+7 58,9 /+.

T58 58,9 /+ =7+ + 8=+>>8< 4+5

89>8,=,8 6/7,/ 89 / /+7 >,86+8 />8,+7 89>8,=,8.

C>6+: - 2 +,85,  O>8,+7 P,8=,8: -

U> / " , 

O>8,+7 T89>8,=,8: -

U> / 2# /C

'. BIOREACTOR  A +/,86/, + 7 97=06=,8< /, 87+788,8< <8;+68 5 =>>/,  +//+6

6+;8 87;+,/7987. B+/,86/, / ;88 +7 45+65  6589+6 >,/68 + 6,,+8< /= 45+65 +7;/;8 /,7+9 /, +/6589+6 6+;8 =768 <8,+;8< 0,/9 =65 /,7+9. T5+ >,/68 67 8+58, 8 8,/+6 /, 78,/+6. T588 +/,86/, ,8 6/99/7 6+7<,+63 ,7+7 +7 +@8 0,/9 +,8 / 6=+6 98,83 7< ,8 /087 9<8 /0 +78 88. I 9 / ,808, /  <8;+68 /, 89 987 / ,/4 68 /, +=8 +7 58 6/78? /0 68 6==,8. T588 <8;+68 ,8 8+7 <8;8/>8< 0/, =8 +7 +=8 87+788,+7 /, +/6589+6 87+788,+7. B+//+6 ,8/=,68 +76=<8 ,+6==,83 0/,8,3 7< +//+6-<8,+;8< 483 7< 58,8 ,8 97 /58, ,87848  +/,8/=,68 8?9>8. O58, 878, /,8 6589+6 +7 >7 +76=<8 /+3 4?83 03 86.3 7< 86=8 588 /58, >7 6/9>/=7< 5;8 <++76 >,/>8,+83 58 /008,   >/87+ 0/,  5/ /0 <+008,87 +/>,/<=6. T58 B+/>,/68 P+/ 6++ (BP) + 7 +78,8< 9=+->=,>/8 06++ 0/,  08,987+/73 +/,70/,9+/7 7< ,/68+7  I7+=8 /0  B+/>,/<=6 D8;8/>987 (IBD). T58 06++ + 5+5 87+788,8< 7< +/,86/, ,8  =+ 66/,<+7 / 6GMP 7<,<. B+/,86/, ;/=98 ,8 +76,88<  8= 8/98, 0/, />+9=9 68 =>. T58 06++ 5 58 ++ / 6,, /= 68,+

08,987+/7 ,7+7 0,/9 1$L / 1#L. T58 06++ + ,=7   +8< 9=+<+6+>+7, 89 /0 87+788, 7< 8657+6 =>>/, 00. B+/,86/, ;+8 +7 IBD3 UTM 1# L B+/,86/,  • 1# L B+/,86/, (2?) • 1$ L B+/,86/, (%?) • 6/98 4+5 =/9+6 89>8,=,83 >H3 CO2 7< >O2 6/7,/8,. M,9+7 C8 C==,8 +/,86/,. IBD 5;8 4/ >8 /0 68 6==,8 +/,86/, 45+65 ,8 <+>/8  >8 7< CSTR  >8. A 58 +/,86/, ,8 8=+>>8< 4+5  7868, 6/7,/ 89 7< 67 8 ,=7 4+5 9+7+9 5=97 =>8,;++/7. A 58 < ,8 /8< / 6/9>=8, 89 7< 67 8 ;+848< 7< 97+>=8< 8,. A 588 06++ + 5/=8< +7 6878< ,//9 7< 8=+>>8< 4+5  7868, 8=+>9873 =65  /4 89>8,=,8 0,88@8,3 +/ 08 6+783 +7;8,8< 9+6,/6/>8 4+5 698, 7< +76=/,.

. CENTRIUGE

A 687,+0=8 +  >+868 /0 8=+>9873 878, <,+;87  7 886,+6 9//, 5 >= 7 /86 +7 ,/+/7 ,/=7<  0+?8< ?+3 >>+7  0/,68 >8,>87<+6=, / 58 ?+. A 687,+0=8 + / =8< / 8>,8 58 6/9>/787 /0 //< +7 //< 7. T58 687,+0=8 4/, =+7 58 8<+987+/7 >,+76+>83 458,8 58 687,+>8 6688,+/7 6=8 <878, =768 / 8>,8 /= /7 58 ,<+ <+,86+/7. B 58 98 /87 +58, /86 4+ 87< / 9/;8 / 58 />.

C87,+0=+/7 +  >,/68  45+65  687,+0=8 + =8< / 8>,8 6/9>/787 /0  6/9>8? 9+?=,8. B >+77+7 /,/, 9>8  ;8, 5+5 >88<3 58 6/9>/787 /0  +;87 9+?=,8 ,8 =868< / 687,+0= 0/,683 45+65 6=8 9/,8 <878 >,+68 / 9+,8 4 0,/9 58 ?+ /0 ,/+/7 7< +58, /78 / 9/;8 /4,< +. T588 >,+68 67 8<+987  58 //9 /0 58 =8 +7/ 45 7/47   >883 7< 5+ +/8< >86+9873 /, 58 ,89+7+7 /=+/73 58 =>8,773 67 8 0=,58, >,/688< /, 78<. T58 >,+76+>8 6/9>/787 /0  687,+0=8 + 58 ,//,3 45+65 + 58 9/;+7 >, 5 >+7  5+5 >88<. M7 687,+0=8 4+ 5;8  ,80,+8,+/7 =7+ 5 /4 58 +78,7 89>8,=,8 / 8 6/7,/8< <=,+7 58 >+7. T58 / 5;8 7/ /, =/7 0/, +7>=+7 58 >,988, 0/, 865 ,=73 45+65 67 +76=<8 58 <=,+/7 /0 58 >+73 58 89>8,=,83 7< 58 97+=<8 /0 58 >+7 +7 8,9 /0 >88< /, ,8+;8 0/,68. S>+7 >88< + 98=,8<  RPM3 /, ,8;/=+/7 >8, 9+7=8.

1*. TURBO E!TRACTOR 

T=,/ 8?,6/, 7< <+++/7 >7 + =80= / 67 8?,6 887+ /+ 0,/9 /6 58, 4+5 5+5 +8<. T58 >7 67 =++@8 8+58, 5<,/-8?,6+/7 >,/68 /, 89 <+++/7 8?,6+/7 >,/68. T58 >7 + 8=+>>8< 4+5 8?>/+/7 >,//0 9//,  =+8 0/, /;87 8?,6+/7 >,/68. T58 >7 67 668> 4+<8 ;,+8 /, ,4 98,+. G878, 80 ,4 98,+ 67 8 >,/688<  + + 58 /58, >8 =65  ,5+@/983 ,88 ,// 7< , 788< / 8 ,/=7<8< / 9 +@8 +7 /,<8, / 98 58 >,/68 />+9+@8<. S>86+ <8+7 +/, + +78< +7 /,<8, / >88< => 58  >,/68. T5+ 4/ >88< +/, 67 57<8 8;87 5,< ,4 98,+. C>6+: !65 88< T>8: A >8 /0 58, L8;8 R5+@/98 T,88 ,// T,88 , E?,6+/7 T>8: -

H<,/ <+++/7 S89 <+++/7 S/;87 <+++/7

O78 /0 58 8?9>8 +7 58 58, 7< ;,+/= /58, 0//< >,8>,+/7 +7<=,+8 + 4587  ;888 9,+? 7868+8 8?,6+/7 0/, 0=,58, >,/68+73 + + +9>/,768 / 87=,8 5  5+5 <8,88 /0 8?,6+/7 800+6+876 + ,8658<3 5 58 8?,6 + 7/ /?+<+8<. M7 /,/, >,/68<=,8 ,8=+,8 /+<-+=+< 8?,6+/7    >,8+9+7, >58 /0 58 >,8>,+/7 /0  9>83 0/, 8?9>8  5>>87 +7 58 7+ /0 87;+,/7987 6/79+77 +7 ;888. T58 />8,+/7 /0 /+<-+=+< 8?,6+/7 9= =,788 5  58 78 ,8 ,8,+8;8< 6/9>88 7< 5 58 ,8 +7 7/ 4 <8,<8< <=,+7 58 8?,6+/7 >,/68. T58 >>+6+/7 /0 T=,/ E?,6/, ,8 6/>878 8?,6+/7 0,/9 +7<=,+ /9/ +7 48 7< 58 ,8,+8; /0 887+ /+ 0,/9 6,> /0 /,78 >88 ,8+<=8 0,/9 67<+8< >88 >,/<=6+/7.

LABORATORIES + DEPARTMENTS VISIT 1. PICTURES AT COSMECEUTICAL * RAGRANCES DEPARTMENT

B,+80+7 * 8?>7+/7  M. N=,5+95 /= ,88,65 6/7<=68<  C/9868=+6 * ,,768 D8>,987.

C589+63 I7,=987 7< E=+>987 +7 C/9868=+6 * ,,768 L.

P,/<=6 /0 C/9868=+6 * ,,7683 IBD: A7+-O8+ C,89

P,/<=6 /0 C/9868=+6 * ,,7683 IBD: V,+/= /0 E87+ O+ 8?,68< 0,/9 /6 0/48,3 58, 7< 8;8

P,/<=6 /0 C/9868=+6 * ,,768: 6+3 B/< * H7< C/98+6 (L/+/73 C878,3 T/778,)

R4 M8,+ =65  >+683 58, 7< <,+8< 0/48, =8 +7 58 ,88,65 7< <8;8/>987 /0 C/98+6 7< P8,0=98.

P,/<=6 /0 C/9868=+6 * ,,768: R/ P8,0=98 9<8  00,7 O+ * G5,= O+

A9/7 58 N+/7 * I78,7+/7 A4,< ,868+;8  58 89 /0 ,88,658,.

. PICTURES AT OOD + NUTRACEUTICAL DEPARTMENT

R4 98,+ =8< +7 58 ,88,65 / <88,9+78 + 7=,++/7 6/787 0/, >,/<=6 <8;8/>987

R4 98,+ =8< +7 58 ,88,65 / <88,9+78 + 7=,++/7 6/787 0/, >,/<=6 <8;8/>987

T58 8=+>987 =8< +7 58 /,/,3 T+7/988, 7< M B768

T58 8=+>987 =8< +7 58 /,/,3 V+6/988, 

R88,65 >,/<=6 C/6/ B=8,

R88,65 >,/<=6 =65  P5/P= T/7 A+3 H8, C5/6/8 7< /58,

A7 A4,< 4+77+7 ,88,65 COGURT- <+,

D+> R6 +7 58 D8>,987 / 8?5++

0,88 7< 5+5 7=,++/= /=, 0,/9 6/6/7=.

 58 ,88,65 >,/<=6 4+5 +70/,9+/7 7< 58 ,86/7++/7 5 58 ,88,658,  ,868+;8 7+/7 7< +78,7+/7. ". PICTURES AT INE CHEMICAL ENGINEERING PILOT PLANT

E=+>987 5 ;+8 +7 IBD P+/ P7

E=+>987 5 ;+8 +7 IBD P+/ P7

E=+>987 5 ;+8 +7 IBD P+/ P7

D,+8< K00+, L+98 8;83 B, /0 G5,= * C87<7 / 8 088< +7/ 58 8?,6/,.

S68 => R/, E;>/,/, 0/, 5+58,   >,/<=6+/7

D,+8< P+78>>8 >/4<8, 08, 58 =+68 8+7  >,/68  58 >, <,8, 

CONCLUSION

IBD 5 887 7/47  + >86++ +7 6/7,+=+7 +<8 0/, ,88,65 7< <8;8/>987 0+8< +7 M+. T5,/=5 5+ ,+>3 =<87 5< +78<  / /0 7/48<8 /7 7+6 985/< 7< 8=+>987 =8< +7 +7<=,+ >,/68. P,/68 =65  8>,+/73 <+++/7 7< 8?,6+/7 5< 887 =8< 9+7 +7 8?,6+/7 /0 887+ /+ 0,/9 58, >7 7< =<87 5;8 887 8?>/8< / 58 >,/68 +80. N/ / 0/,8 5,/=5 5+ ,+> =<87 8 / 7/4 58 965+78 0,/9 00 /0 IBD 7< 58+, 6/7,+=+/7 7/ /7 / 6/98+6 0+8< = / / 98<+6 0+8<. T58 +7<=,+ ;++ / 8?>/8< =<87 / 8,7+7 >,/68 <=,+7 +7<=,+ ,+7+7. B8+<83 =<87 8,78< 58 >,/68 /, 985/< =8< / >,/<=68 ,8 68  >,/<=6+/7 /0 >,/<=6 =65  65 >,/68. I7 6/76=+/73 58 +7<=,+ ;++ 5 ,8658< + /86+;8 / 8?>/8 =<87 /4,< 965+78 7< >,/68 =7+ 4/, =8< +7 8>,+/73  +/6589+, >,/68 7< 6589+6 ,86+/7 87+788,+7. I7 <<++/73 =<87 8 / /8,;8 58 4/,+7 87;+,/7987 +7 +7<=, 7< 58 >,6+6 4/, =8< +7 +7<=,+ ,+7+7.

SEPARATION • • • • • •

S/?58 E?,6/,  R/, E;>/,/,  S>, D,8,  M/86=, D+++/7 T=,/ E?,6/,  C87,+0=8

CHEMICAL REACTION •

ENGINEERING H+5 P,8=,8

ANALYTICAL •

L+=+< C5,/9/,>5-

•

R86/,  H+5 P,8=,8 L//>

(LCMS)

•

,86/,  B+/,86/,

REERENCES 1. P,+76+>8 /0 ,/, 8;>/,/, 

M S>86,/98,

,/9: 5>:+8+70/.6/9+70/2121=8-7<->,+76+>8-/0-58-,/,8;>/,/,.59 (R8,+8;8 /7: 2' M,65 21") 2. H+5 P8,0/,9768 L+=+< C5,/9/,>5 (HPLC)  ,/9: 5>:444.6589=+<8.6/.=7+65,/9/,>55>6.59 (R8,+8;8 /7: 2' M,65 21") !. L+=+< C5,/9/,>5  M S>86,/98, ,/9: 5>:444.>5,968=.<869 (R8,+8;8 /7: ! A>,+ 21") ". L+=+< C5,/9/,>5  M S>86,/98, ,/9: 5>:444.+6.6.=8,;+6898//9+6/>+66945.59 (R8,+8;8 /7: ! A>,+ 21") #. A/= I7+=8 /0 B+/>,/<=6 D8;8/>987 UTM  ,/9: 5>:444.784+<.=9.9/=/=-+< (R8,+8;8 /7 ! A>,+ 21") $. +78 C589+6 E7+788,+7 P+/ P7 ,/9: 5>:444.784+<.=9.98,0+78-6589+6->+/->7-8=+>987 (R8,+8;8 /7: ! A>,+ 21") %. B+/>,/68+7 P+/ 6++ ,/9: 5>:444.784+<.=9.9,7<+/>,/68+7->+/-06++ (R8,+8;8 /7 " A>,+ 21") &. //< * N=,68=+6 ,/9: 5>:444.784+<.=9.9,7<<8>,987-/0-,88,65-+7/;+/70//<7=,68=+6 (R8,+8;8 /7 " A>,+ 21") '. G, D. C5,++7 Analytical Chemistry $5 E<++/7. W+8 I78,7+/7 P=+6+/73 U7+8< S8 /0 A98,+6. 1. C5,++8 J. G87/>+ Transport Process and Separation Process Principles. "5 E<++/7. P8,/7-P,87+68 H E<=6+/7 I76. N84 J8,8 USA. 11. H.

S6/ /8,. Elements of Chemical Reaction Engineering "5 E<++/7. P,87+68 H I78,7+/7 S8,+8. USA