AIRBORNE CONTAMINANTS
ALTERNARIA
AS PERGILL US
BOTYRIS
CHAETOMI UM
CE PHALOSPORI UM
CURVULARI A
FUSARI UM
Rapid-growing cooni!"# gra$i"% &o 'ac( &o 'rown) *nd!r"id! +!& 'ac(
Fa&# co,pac& cooni!"# w%i&! a& ir"& &%!n '!co,ing 'ac(# gr!!n# '*i"% or $!ow
So& So& oo(in oo(ing g &an &o &o gra$ gra$ coon$ coon$
Sow Sow growing growing## a&# a&# w%i&!# w%i&!# $!ow# &an or 'rown coon$
.%i&! &o &an &o ro"!-coro!d coon$# !/!n&*a$ d!/!oping .%i&! a!ria %$p%a!0
1ar( 'rown &o 'ac( coon$ on 'o&% "id!" ragg!d in app!aranc!0
Fa"&-growing coon$0 A& ir"&# w%i&! and co&&on$ '*& d!/!ping ro"! &o r!d coor on 'o&% "id!"
Larg!# %and gr!nad!-"%ap!d "por!" wi&% 'o&% ongi&*dina and &ran"/!r"! cro"" wa"0 Born! "ing$ or in c%ain"0 S!p&a&!# d!,a&iac!o*" *ngi0
S,a on!-c!!d "por!" irradia&ing o*& ro, "wo!n 'a"! 2"!! arrow"3
Coor!""# on! c!!d "por!" 'orn! in c*,p"
Larg!# dar(# c!n&ra "&r*c&*r! i" p!ri&%!ci*, &%a& con&ain" a"co"por!" 2"!4*a$ prod*c!d3
Sing! c!!d# c!ar# !ip&ica "por!" %!d &og!&%!r in a 'a *n!"" 'ro(!n oo"!
Larg!# '!n& "por!" wi&% 5 &o 6 c!"0 Si,iar &o H!,in&%o"pori*, "pp0 Brown# "!p&a&! %$p%a!0
Larg!"& "por!" ar! "ic(!-"%ap!d and ,a$ con&ain "!/!ra c!"0 S,a "por!" wi&% on! &o &wo c!" %a/! ,or! ro*nd!d !nd"0
CULTURE
MORPHOLOGY
MICROSCOPIC VIE.
1ESCRIPTION
AIRBORNE CONTAMINANTS
GELASINOS PORA
GEOTRICHUM
GLI OC LA1I UM
HORMO1EN1RUM
NEUROS PORA 2M 2MONILI A3
NI GROSPORA
PAECI LOMY CES
1ar( coon$ wi&% n*,!ro*" ",a 'ac( do&" w%ic% ar! p!ri&%!cia 2"!4*a organ" or A"co,$c!&!"3
.%i&! &o &an# a& or *$# rapid-growing *ng*"
Fa&# rapid-growing coon$0 .%i&! a& ir"&# &%!n d!/!oping dar( gr!!n c!n&ra por&ion
Gr!!n &o gra$ &o 'ac( coon$ on 'o&% "id!"0 O&!n wrin(!d and grow" a&
.%i&! a& ir"& '*& grow" rapid$ iing &%! !n&ir! P!&ri di"% in a !w da$" and '!co,ing a "a,on &o 'rown coor0 M$c!i*, ,a$ %ang ro, &%! id o &%! P!&ri di"%0
Rapid growing# prod*cing a'*ndan& *$# a!ria %$p%a!# gra$ &o 'ac( on 'o&% "id!"0 R!"!,'!" M*cor or R%i7op*"
Fa&# rapid-growing# &an-coor!d coon$ r!"!,'ing A"p!rgi*" "pp0
S8*a"%!d p!ri&%!ci*, "%owing ,an$ a"ci# !ac% con&aining 9 "!4*a$ prod*c!d a"co"por!"
No&! %$p%a! 'r!a(ing in&o ar&%ro"por!"0 Ma$ '! con*"!d wi&% Coccidioid!" i,,i&i"0
N*,!ro*" ",a "por!" %!d &og!&%!r in a c*,p0 Si,iar &o P!nicii*, "pp0 E4c!p& or &%! c*,ping o "por!"0
1ar( 'rown "!p&a&! %$p%a! '!ar 'ranc%ing c%ain" o !onga&! &o o/a&! "por!" &%a& o&!n con&ain a ",a 'ac( do& a& &%! !nd0 Spor! '!aring "&r*c&*r!" oo( &r!!-i(!
C!ar "!p&a&! %$p%a! wi&% arg! ,a""!" o o/a&! "por!" w%ic% ar! air-di"p!r"!d
Larg!# c!ar$ /i"i'! +!& 'ac( "por!"0
Si,iar &o P!nicii*, "pp0 !4c!p& ",a "por!" ar! prod*c!d on /!r$ ong# "!nd!r "&r*c&*r!"
CULTURE
MORPHOLOGY
MICROSCOPIC VIE.
1ESCRIPTION
AIRBORNE CONTAMINANTS
PENICILLIUM
PULLULARIA 2A*r!o'a"idi*,3
RHO1OTORULA
RHI:OPUS
SACCHAROMYCES
SCOPULARIOPSIS
SEPE1ONIUM
.%i&! coon$ a& ir"& '*& d!/!oping '*! &o gr!!n coor
Brown &o 'ac(# a&# gr!a"$oo(ing cooni!"
A pa! $!ow &o /i/id orang! $!a"&
Gra$ &o 'rown &o 'ac( coon$ iing a P!&ri di"% in ; &o 5 da$"0 Si,iar &o Mucor "pp0
.%i&! &o &an $!a"& cooni!"
Powd!r$# ig%& 'rown# wrin(!d coon$ r!"!,'ing P!nicii*, "pp0 E4c!p& or coor0
F*$# w%i&! coon$ r!"!,'!" Hi"&opa",a cap"*a&*,
S,a# ro*nd "por!" 'orn! in <'r*"%-i(!= or,a&ion"
Mi4&*r! o dar(# &%ic(-wa!d# arg! %$p%a c!" and c!ar "por!" w%ic% "!!, &o '! '*dding
Long# "!nd!r $!a"& c!"# *"*a$ '*dding# '*& ,a$ app!ar &o or, p"!*do%$p%a!0
Si,iar &o Mucor "pp0 !4c!p& oo&i(! "&r*c&*r!" 2r%i7oid"3 a& 'a"! o "por! '!aring %$p%a! 2"!! arrow"30 Spor!" in "porangi*, c! ar# co!noc$&ic %$p%a!
T$pica $!a"& c!" &%a& r!prod*c! '$ '*dding0
R!"!,'!" P!nicii*, "pp0 !4c!p& "por!" ar! arg!r and or, *n'ranc%!d-c%ain"
Larg!# ro*g%-wa!d "por!" &%a& r!"!,'!" Hi"&opa",a cap"*a&*,
CULTURE
MORPHOLOGY
MICROSCOPIC VIE.
1ESCRIPTION
AIRBORNE CONTAMINANTS
SPOROBOLOMYCES
STEMPHYLUM
STREPTOMYCES
SYNCEPHALASTRUM
VERTICILLIUM SUPERFICIAL
CULTURE
1!r,a&op%$&o"!" > d!r,a&op%$&!" G!n*" Micro"por*, G!n*" Epid!r,op%$&on G!n*" Tric%op%$&on • • •
Pi&$ria"i" V!r"icoor > Maa77!7ia -*r-*r
A ig%& &an $!a"& coon$ MORPHOLOGY
Brown &o 'ac(# wrin(!d *77$ coon$
1r$# a&# !a&%!r$ coon$ grow" co"! &o &%! ,!di*,
Gra$ &o 'rown &o 'ac( *$ coon$ &%a& ,a$ i a P!&ri di"% in a !w da$"0 Si,iar &o M*cor and R%i7op*" "pp
Powd!r$ &o pin(i"% 'rown coon$ &%a& oo(" "i,iar &o P!nicii*, "pp0
Tinianigra > Cado"pori*,w!rn!c(ii Pi!dra w%i&! > Tric%o"por*,2'!ig!i3 c*&an!*, 'ac( > Pi!draia %or&ai
• •
MICROSCOPIC VIE.
O&o,$co"i" A"p!rgi*" M*cor P!nicii*, R%i7op*" • • • •
?!ra&i&i"
1ESCRIPTION
Y!a"& c!" &%a& ,a$ or, ong "!nd!r &*'!"0 R!prod*c!" '$ '*dding
H$p%a! ar! 'rown and "&rong$ "!p&a&!@ %*g! ,*&ic!!d &!r,ina "por!" &%a& ,a$ !i&%!r '! ",oo&% or ro*g%
V!r$ in! 2*,3 'ranc%ing %$p%a! &%a& r!adi$ 'r!a( apar&
Broad# c!ar# non"!p&a&! %$p%a!# "por!" in ,an$ "!nd!r "ac-i(! "&r*c&*r!" 2"porangia3 ad%!r! &o a "w!ing on &%! &!r,ina !nd o %$p%a0
F*"ari*, A"p!rgi*" C*r/*aria P!nicii*, C!p%ao"pori*, Candida "p!ci!"
•
Sing!-c!!d !ip&ica "por!" 'orn! in c*"&!r" &%a& ar! arrang!d in w%or"
• • • • •
1ERMATOPHYTES
MICROSPORUM GYPSEUM
MICROSPORUM NANUM
MICROSPORUM VANBREUSEGHEMII
MICROSPORUM AU1OUINII
MICROSPORUM CANIS
MICROSPORUM 1ISTORTUM
MICROSPORUM FERRUGINEUM
Cultured on Sabouraud plus 2 antibiotics at room temp. for 2-3 weeks. Fluffy white colony with slight yellow underside
Cultured on Sabouraud medium plus 2 antibiotics at room temp. for 1-2 weeks. White on top with bright yellow underside.
Culture on Sabouraud medium plus 2 antibiotcs at room temperature for 3 weeks. Similar to icrosporum canis but with less pigmentation.
Cultured on Sabouraud medium plus 2 antibiotics for 3 weeks at room temp. White to intense orange yellow strains! often sectors
Cultured on Sabouraud medium plus 2 antibiotics for "-1# days at room temp. $rows rapidly% producing a cinnamon to brown colored flat colony.
Cultured on Sabouraud mediu m plus 2 antibiotics at room temp. for 1-3 weeks. White to buff surface& bottom often yellow red brown.
Cultured on Sabouraud medium plus 2 antibiotics for 1-2 weeks at room temp. Cottony white surface% may de'elop pink to tan coloration! bottom often colorless to yellow.
1ESCRIPTION
(o distinguishing spores will not grow on rice
)ighly diagnostic large% thickwalled% rough spores containing more than * septa.
+arge spores similar to icrosporum canis but distorted and bent in shape.
(o distinguishing spores. ,rominent septa% gi'ing term bamboo hyphae.
(umerous% characteristic% +arge spore! thin-walled% pointed ends with 2-" septa.
/gg-shaped% thin- walled! large spores with 1-3 septa
)uge% long% thick rough- walled spores with more than 0 septa.
TRICHOPHYTON VIOLACEUM
TRICHOPHYTON CONCENTRICUM
TRICHOPHYTON EUINUM
TRICHOPHYTON MENTAGRPPHYTES
TRICHOPHYTON RUBRUM
1ERMATOPHYTES
TRICHOPHYTON SCHOENLEINII
TRICHOPHYTON TONSURANS
CULTURE
MORPHOLOGY
MICROSCOPIC VIE.
CULTURE
1ERMATOPHYTES
SUBCUTANEOUS
CULTURE
TRICHOSPORON BEIGELII 2roo, &!,p!ra&*r! c*&*r!"3 SPOROTHRI SCHENC?II 2Roo, T!,p3
EPI1ERMOPHYTON FLOCCOSUM SPOROTHRI SCHENC?II 25D C c*&*r!"3
SUBCUTANEOUS
PIE1RAIA HORTAI 2roo, &!,p c*&*r!"3
MALASSE:IA FURFUR FONSECAEA PE1ROSOI
Sporo&ric%o"i"> Sporo&%ri4 "c%!nc(ii
FONSECAE COMPACTA
PHIALOPHORA VERRUCOSA
CULTURE
MORPHOLOGY MORPHOLOGY
MICROSCOPIC VIE. MICROSCOPIC VIE.
1ESCRIPTION 1ESCRIPTION
SUBCUTANEOUS
Cultured on Sabouraud T%! cooni!" ar! ",oo&% &o medium on 2 antibiotics for 1-3 "ig%&$ wrin(!d 2no&c*&*r! *77$3 1ar(# gr!a"$-oo(ing .%i&! &o &an $!a"& cooni!" a&!r R weeks at room temp. ellow to and w%i&! &o &an in coor a&!r &o ; w!!(" inc*'a&ion on &o 5 w!!(" inc*'a&ion on 'rain O green colored surface! green to L Sa'o*ra*d ,!di*, %!ar& in*"ion agar0 brown underside. O C I S R E V S I S A I R Y T I +arge% club-shaped spores with On! o'"!r/!" c!ar "!p&a&! P %$p%a! w%ic% ,a$ 'r!a( *p C!ar# "!p&a&! c!"0 %$p%a! wi&% in&o indi/id*a
"por!" 25 &o *,3 in
N BRASILIENSIS 2TOP3 N MA1UARAE2BOTTOM3-RT
2 to " septa% often form in Ro*nd# o/a# or
$!a"& c!"# &o 5 *, 4 &o *,
EOPHIALA 2.ANGIELLA3 1E RM ATI TI 1I S
T%! cooni!" ar! dar( 'rown Cooni!" in 1i4onJ" agar ar! and ,a$ %a/! a ,!&aic gr!!n cr!a, &o S*rac! i" $!owi"%# dar( gr!!n#",oo&% gra$ oror S*rac! i" dar( gr!!n &o 'ac(0 &ing!0 T%!$ ar! /!r$ co,pac&# ig%&$ wrin(!d# gi"&!ning or 'ac(# co/!r!d '$ "i/!r$# /!/!&T%! cooni!" ar! %!ap!d# 'ri&&! %a/! a rai"!d c!n&!r and ar! d*# wi&% &%! ,argin '!inga&# i(! ,$c!i*,0 I& i" *"*a$ wi&% irr!g*ar ind!n&!d 'ord!r"0 "ig%&$ *77$0 !i&%!r !n&ir! or o'a&! &%!n d!/!op" a con! "%ap!d A T%!r! ar! 'rowni"% %$p%a! on pro&r*"ion in &%! c!n&!r0 R!/!r"! i" R &%! "*rac! 1 On! o'"!r/!" d!,a&iac!o*" 'ac(0
E I P
K ?OH wi&% Par(!r in( ,o*n& "%owing c%arac&!ri"&ic "p%!rica $!a"& c!" and "%or& T%! %$p%a! i"!!,!n&" "!p&a&!#&$pica 'ranc%!d p"!*do%$p%a and 'rown0 o &%! *ng" T%! conidia i" dar(
,!a"*ring 06-50 4 ;06-0 *,0
C ON I1 IO BL OU S C OR ON AT US 2 ;6 -5 C 3
%$p%a! con&aining n*,!ro*" "!p&a
H$p%a! i" "!p&a&!# 'rown# 'ranc%ing and '!ar Cado"pori*, o conidiop%or!"0 O*&"&anding !a&*r!" ar! a"(i(! "%ap! conidia and co,pac& arrang!,!n& o conidia c%ain0
E OP HI AL A EA NS EL ME I
T%! %$p%a! i" 'rown# 'ranc%!d and "!p&a&! wi&% /a"!-"%ap!d P%iaop%ora &$p! conidiop%or!"0 T%! conidiop%or!" ar! "ing! or ,*&ip!# a&!ra or &!r,ina and '!ar !a"i$ di"r*p&!d ,a""!" o o/a conidia
P%iaop%ora /!rr*co"a Fon"!ca! p!dro"oi Cado"pori*, carrioni M$c!&o,a or Mad*ro,$co"i" Ac&ino,$co&ic Ac&ino,ad*ra! Nocardia T%! coon$ %a" a dar(S&r!p&o,$c!" "*rac!# a& Cooni!" ar! a&# $!owi"% gra$ wi&% "ig%&$ rai"!d c!n&!r0 I& i" &o cr!a,$ gra$# ga'ro*" E*,$co&ic2&r*! -*ngi3 co/!r!d wi&% /!/!&$ d* gra$# '!co,ing radia$ od!d and A!"c%!ria2or gra$ gr!!n or p*rpi"% 'rown# "%or& P!&ri!idi*,3 co/!r!d '$ a in! powd!r# w%i&! napp!d ,$c!i*,0 R!/!r"! i" Mad*r!a "*rac! ,$c!i*, 'ac(0 P%iaop%ora En&o,op&%oro,$co"i" Ba"idio'o*" ranar*, Conidio'o*" corona&*" Lo'o,$co"i" > Laca7iao'oi2Lo'oa o'oi3 R%ino"poridio"i" > R%ino"poridi*, "!!'!ri P%a!o%$po,$co"i" .angi!a "pp T%! %$p%a! i" "!p&a&!# dar( wi&% T%!r! i" &%! pr!"!nc! o arg! E4op%iaia "pp a&!ra and &!r,ina conidiop%or!" /!g!&a&i/! %$p%a! or,ing "pp0 o /ar$ing Cado"pori*, "i7!0 conidiop%or!" n*,!ro*" ro*nd# ",oo&%# &%ic( prod*c! ong 'ranc%ing c%ain""pp0 o Hor,od!ndr*, wa!d 7$go"por!" wi&% &wo 'rown# ",oo&% wa!d# o/a# 1r!"c%!ra "pp0 co"!$ appr!""!d '!a(i(! poin&!d conidia w%ic% %a/! dar( "car" o a&&ac%,!n&
app!ndag!"
P SE U1O AL LES CH ERI CH IA B OY 1I I 2 SE U AL 3 he colonies ha'e a cottony surface that is white to gray-brown in color and gets darker with age of the culture. he re'erse is also white turning brown with age.
CULTURE
MORPHOLOGY
S*rac! i" dar( gr!!ni"%# 'rown &o 'ac( wi&% a co"! ,a&i(!# oi/! &o gra$ ,$c!i*,0 So,! "&rain" ar! %!ap!d# gran*ar or a& wi&% a ,a&&!d app!aranc!0 R!/!r"! i" 'ac(0
C%ro,o,$co"i" CLA1OSPORIM CARRIONIor C%ro,o'a"&o,$co"i" BASI1IOBOLUS RANARUM
Sow growing# dr$app!aring cooni!" on Sa'o*ra*d ,!di*,0 Morp%oog$ /ari!" '!&w!!n !&ioogic ag!n&"0 U"! 'ioc%!,ica &!"&"0
Colonies are slow growing% initially black and yeastlike% becoming suede-like% o'ilaceous grey and mould like age.
Colonies are flat% cream-colored% glabrous becoming radially folded and co'ered by fine powdery white surface myceli um and conidiophores.
T%! cooni!" ar! 'rown or gr!!n-'ac(# ,oi"& and gi"&!ning0 .i&% ag! &%!$ '!co,! co/!r!d wi&% /!/!&$ gra$i"% %$p%a!0 T%! r!/!r"! i" 'ac(
MICROSCOPIC VIE.
GRAPHIUM# a"!4*a "&a&! o P0 BOY1II 2"!4*a "&a&!30 T%! cor!,ia or S$nn!,a&a2conidia "&r*c&*r!"3 o &%! Grap%i*, "&a&! o P0'o$dii %a/! &!r,ina %$ain! conidia# c*'-"%ap!d or c$indrica# appro4i,a&!$ 4 5 *,0 In &%! "!4*a "&a&!2P0'o$dii3# arg!# 6-; *, in dia,!&!r# ro*nd# 'rown c!i"&o&%!cia ar! o*nd con&aining a"co"por!"
SCE1OSPORIUM APIOSPERMUM 2ASEUAL STATE OF BOY1II3 T%! Sc!do"pori*, &$p! o conidia o P0 'o$dii ,a$ ri"! dir!c&$ ro, &%! "!p&a&! %$p%a! or ro, &%! &ip o conidiop%or!"# app!ar &r*nca&!d a& &%! 'a"!# and "o,!&i,!" r!"!,'! &%! conidia o Ba"&o,$c!" d!r,a&i&idi"0 T%! %$p%a! ar! ong and "!nd!r# 'ranc% a& ac*&! ang!" and &%*" ,a$ r!"!,'! a"p!rgii0
Varia&ion" in acid a"&n!""
n new cult ure% o'al and
he
hyphae
ha'e
few
septa.
he
T%! conidiop%or!" ar!
1ESCRIPTION
%!p &o d!&!r,in! '!&w!!n "o,! !&ioogic ag!n&"0 1!ica&! %$p%a!# *,0 A ar! ,!,'!r" o &%! Ac&ino,$c!&!"0 R!co,,!nd!d roo, &!,p!ra&*r! inc*'a&ion# a&%o*g% ,orp%oog$ i" "i,iar a& 'o&% &!,p
1EEP SEATE1 Hi"&opa",o"i" > Hi"&opa",a cap"*a&*,
round budding yeast-like cells are formed. Subse4uently these cells produce septate hyphae with flask-shaped to cylindrical phialides found at the tip of the phialide and also along d hyphae.
1EEP SEATE1
conidiophores are unbranched forming solitary terminal conidia. he conidia are spheric al% single-celled and ha'e a prominent papilla. t may also produce hairlike appendages called 'illae.
HISTOPLASMA CAPSULATUM ROOM TEMP
!onga&!d# &*'*ar and wi&% a &ap!r!d# narrow !nd0 Conidia ar! ",oo&%# &%inwa!d# and !ip"oid and can ga&%!r in c*"&!r" aro*nd &%! conidiop%or!" and a& poin&" aong &%! "!p&a&! %$p%a!
5D C
RHINOSPOR T%i" organi", %a" n!/!r '!!n c*&*r!d) i&" !4i"&!nc! a" a wa&!r "aprop%$&! or i"% pa&%og!n i" "*"p!c&!d I1IUM SEEBERI 2RT AN1 5D C3
COCCI1IO1ES IMMITIS
PARACOCCI1IOI1ES BRA:ILIENSIS RT
5D C
CULTURE
Coccidioido,$co"i" > Coccidioid!" i,,i&i" So*&% A,!rican Ba"&o,$co"i" > Paracoccidioid!" 'ra"ii!n"i"
MORPHOLOGY
Nor&% A,!rican Ba"&o,$co"i" > Ba"&o,$co"i" d!r,a&i&idi"
.%&i!# innoc!n& oo(ing 2'*& dang!ro*"3 coon$ a&!r -5 w!!(" inc*'a&ion on Sa'ora*d ,!di*,0 No&! coor o *nd!r"id!
.%i&! &o &an $!a"& coon$ a&!r &o 5 w!!(" inc*'a&ion on 'rain %!ar& in*"ion agar0
Innoc!n& oo(ing# '*& dang!ro*" w%i&! *$ coon$ on Sa'o*ra*d ,!di*, a&!r &o 5 w!!(" inc*'a&ion0 Und!r"id! ,a$ d!/!op dar(!r coor
.%i&!# "ow growing# nond!"crip& coon$ on Sa'o*ra*d ,!di*, a&!r ; &o w!!(" inc*'a&ion
Ro*g%# dr$ $!a"& cooni!" a&!r ; &o w!!(" inc*'a&ion on 'rain %!ar& in*"ion agar
C%arac&!ri"&ic arg! &*'!rc*a&!d ,acroconidia 2-; *,3 and occa"iona ",a!r# in!c&io*" ,icroconidia
N*,!ro*"# ",a 2; &o *,3# o/a# or ro*nd# '*dding $!a"& c!"
H$p%a! or,ing c%arac&!ri"&ic ar&%ro"por!" 4 9 *, Grow" "par"!$ a&5D# "a,! ,orp%oog$ a& 5D and RT
Fin!# "!p&a!# c!ar %$p%a! ac(ing "por!"
Larg! $!a"& c!" 2 &o *,3 wi&% n*,!ro*"# ",a '*d" ; &o 6 *,
MICROSCOPIC VIE.
1ESCRIPTION
1EEP SEATE1
BLASTOMYCES 1ERMATITI1IS RT 5D C
OPPORTUNISTIC
CULTURE
CAN1I1A ALBICANS RT AN1 5D C
CRYPTOCOCCUS NEOFORMANS
ASPERGILLUS SP RT 5D C
RHI:OPUS
.%i&! cooni!" inc*'a&!d on Sa'o*ra*d ,!di*, or 6 &o D da$"
O w%i&!# ,*co*" cooni!" on Sa'o*ra*d ,!di*, a&!r 6 &o da$"
Upp!r !&@ gr!!n coon$ a&!r 6 &o da$" on Sa'o*ra*d ,!di*,0 Coor!"" *nd!r"id!
A !&ioogic ag!n&" rapid$ i P!&ri di"% a&!r ; &o da$" inc*'a&ion0 B!co,!" &an or gra$
C%a,$do"por! agar# &o ; da$" inc*'a&ion No&! $!a"& c!"# p"!*do%$p%a! and c%a,$do"por!" U"*a$ c*&*r!d a& RT0 So,! "p!ci!" grow a& 5D C and %a/! ,orp%oog$ "i,iar &o &%a& o organi"," grown a& RT
India in( pr!para&ion "%owing n*,!ro*" %!a/i$ !ncap"*a&!d $!a"& c!"
T$pica
C%arac&!ri"&ic !a&*r!" > "porangi*,# co!noc$&ic %$p%a! and roo&i(! "&r*c&*r!" Op&i,a inc*'a&ion &!,p i" 5 C0 ,orp%oogica$ "i,iar a& an$ &!,p
Candidia"i" > Candida a'ican" Cr$p&ococco"i" > Cr$p&ococc*" n!oor,an"
MORPHOLOGY
Coon$ a&!r &o 5 w!!(" inc*'a&ion on Sa'o*ra*d ,!di*,0 .%i&! coon$# 'rown &o &an *nd!r"id!
Ro*g%# dr$# %!ap!d-*p $!a"& coon$ a&!r &o 5 w!!(" inc*'a&ion on 'rain %!ar& in*"ion agar
:$go,$co"i" 2M*cor,$co"i"3 P%$co,$co"i" R%i7op*" M*cor A'"idia Ba"idio'o*" Conidio'o*"
MICROSCOPIC VIE.
1ESCRIPTION
A"p!rgio"i" > A"p!rgi*" *,iga&*"
1!ica&!# "!p&a&! %$p%a! '!aring o/a &o p$rior, "por!"# &o 9 *,
Y!a"& c!"# &o 6 *,0 No&! 'road-'a"!d '*dding
G!o&ric%o"i" > G!o&ric%*, candid*, P!nicio"i" > P!nicii*, "pp0
OPPORTUNISTIC
BASI1IOBOLUS RANARUM
CONI1IOBLOUS CORONATUS 2;6-5 C3
GEOTRICHUM
U"*a$ c*&*r!d a& 5D C &o in%i'i& con&a,inan&"
PENICILLIUM
CULTURE
MORPHOLOGY
Cooni!" ar! a&# $!owi"% gra$ &o cr!a,$ gra$# ga'ro*" '!co,ing radia$ od!d and co/!r!d '$ a in! powd!r# w%i&! "*rac! ,$c!i*,
Colonies are flat% cream-colored% glabrous becoming radially folded and co'ered by fine powdery white surface mycelium and conidiophores.
.%i&! &o &an# a& or *$# rapidgrowing *ng*"
U"*a$ c*&*r!d a& roo, &!,p!ra&*r!0 So,! "p!ci!" grow a& 5D C and %a/! ,orp%oog$ "i,iar &o &%a& or organi"," grown a& roo, &!,p!ra&*r!
.%i&! coon$ a& ir"& '*& d!/!oping '*! &o gr!!n coor
MICROSCOPIC VIE.
P HO T SA ND T E X T P R O V I DE B Y MICROBIOL GYDEPARTMENT – US TF MS ‘ 04 -’ 05 fo!"#$%&'(A)%*M"#+,$.SEC- /
1ESCRIPTION
T%!r! i" &%! pr!"!nc! o arg! /!g!&a&i/! %$p%a! or,ing n*,!ro*"
he hyphae ha'e few septa. he conidiophores are unbranched forming solitary terminal conidia. he conidia are spherical% single-celled and ha'e a
No&! %$p%a! 'r!a(ing in&o ar&%ro"por!"0 Ma$ '! con*"!d wi&%
S,a# ro*nd "por!" 'orn! in <'r*"%i(!= or,a&ion"
ro*nd# ",oo&%# &%ic( wa!d 7$go"por!" wi&% &wo co"!$ appr!""!d '!a(i(! app!ndag!"
prominent papilla. t may also produce hair-like appendages called 'illae.
Coccidioid!" i,,i&i"0
