MW-12A

MW-12A Microplate Washer

Service Manual

 © 2007-2010 Shenzhen Mindray Bio-Medical Electronics Co., Ltd. All rights Reserved. For this Operator’s Manual, the issue date is 2010-04.

Intellectual Property Statement SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS ELECTRONICS CO., LTD. (hereinafter (her einafter called Mindray) owns the intellectual property rights to this Mindray product and this manual. This manual may refer to information protected by copyright or patents and does not convey any license under the patent rights or copyright of Mindray, or of others. Mindray intends to maintain the contents of this manual as confidential information. Disclosure of the information in this manual in any manner whatsoever without the written permission of Mindray is strictly forbidden. Release, amendment, reproduction, distribution, rental, adaptation, translation or any other derivative work of this manual in any manner whatsoever without the written permission of Mindray is strictly forbidden.

,

,

,

,

BeneView,

WATO, WATO, BeneHeart, are the trademarks, registered or otherwise, of Mindray in China and other countries. All other trademarks that appear in this manual are used only for informational or editorial purposes. They are the property of their respective owners.

Responsibility on the Manufacturer Party Contents of this manual are subject to changes without prior notice. All information contained in this manual is believed to be correct. Mindray shall not be liable for errors contained herein nor for incidental or consequential damages in connection with the furnishing, performance, or use of this manual. Mindray is responsible for safety, reliability and performance of this product only in the condition that: 





all installation operations, expansions, changes, modifications and repairs of this product are conducted by Mindray authorized personnel; the electrical installation of the relevant room complies with the applicable national and local requirements; the product is used in accordance with the instructions for use.

WARNING: It is important for the hospital or organization that employs this equipment to carry out a reasonable service/maintenance plan. Neglect of this may result in machine breakdown or injury of human health.

i

NOTE: This equipment is to be operated only by medical professionals trained and authorized by Mindray or Mindray-authorized distributors.

Warranty THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES, WARRANTIES, EXPRESSED OR IMPLIED, INCLUDING WARRANTIES OF MERCHANTABILITY OR FITNESS FOR ANY PARTICULAR PARTICULAR PURPOSE.

Exemptions Mindray's obligation or liability under this warranty does not include any transportation or other charges or liability for direct, indirect or consequential damages or delay resulting from the improper use or application of the product or the use of parts or accessories not approved by Mindray or repairs by people other than Mindray authorized personnel. This warranty shall not extend to: 





any Mindray product which has been subjected to misuse, negligence or accident; any Mindray product from which Mindray's original serial number tag or product identification markings have been altered or removed; any product of any an y other manufacturer.

Return Policy Return Procedure In the event that it becomes necessary to return this product or part of this product to Mindray, the following procedure should be followed: 1

Obtain return authorization: Contact the Mindray Service Department and obtain a Customer Service Authorization (Mindray) number. The Mindray number must appear on the outside of the shipping container. Returned shipments will not be accepted if the Mindray number is not clearly visible. Please provide the model number, serial number, and a brief description of the reason for return.

2

Freight policy: The customer is responsible for freight charges when this product is shipped to Mindray for service (this includes customs charges).

3

Return address: Please send the part(s) or equipment to the address offered by Customer Service department

Company Contact

ii

Manufacturer:

Shenzhen Mindray Bio-Medical Electronics Co., Ltd.

Address:

Mindray Building, Keji 12th Road South, Hi-tech Industrial Park, Nanshan, Shenzhen 518057, P.R.China P.R.China

Tel:

+86 755 26582479

26582888

Fax:

+86 755 26582934

26582500

Preface Who Should Read This Manual The manual is written for f or service professionals authorized b y Mindray. Mindray.

Conventions Used in This Manual Safety Symbols This chart explains the symbols that may be used in this manual. When you see …

WARNING:

BIOHAZARD:

CAUTION:

NOTE:

Then …

Read the statement following the symbol. The statement is alerting you to an operating hazard that can cause personal injury. Read the statement following the symbol. The statement is alerting you to a potentially biohazardous condition. Read the statement following the symbol. The statement is alerting you to a possibility of system damage or unreliable results. Read the statement following the symbol. The statement is alerting you to information that requires your attention.

EC Representative Representative Name:

Shanghai International Holding Corp. GmbH (Europe)

Address:

Eiffestraβe 80, 20537 Hamburg Germany

Phone:

0049-40-2513175

Fax:

0049-40-255726

Preface

1

Contents Preface............. Preface........................ ....................... ....................... ...................... ....................... ....................... ...................... ...................... ................... ........1 1 Who Should Read This Manual.......................................................................................... Manual.......................................................................................... 1 Conventions Used in This Manual...................................................................................... 1

1

System System Features Features ...................... ................................. ....................... ....................... ...................... ...................... ................1-1 .....1-1

2

System System Installatio Installation n ...................... ................................. ...................... ....................... ....................... ...................... ............2-1 .2-1

3

System System Description Description ....................... .................................. ...................... ...................... ....................... ......................3-1 ..........3-1 3.1

Workflow ..................................................................... ............................................................................................................... .......................................... 3-3

3.2

Components ........................................................................... ......................................................................................................... .............................. 3-3 3.2.1 3.2.2 3.2.3 3.2.4

4

Hardware Hardware Boards Boards ....................... .................................. ...................... ....................... ....................... ...................... ..............4-1 ...4-1 4.1

Main Control Board............................................................................................... 4-3 4.1.1 4.1.2

5

Plate Carrier Assembly ....................................................................... ............................................................................ ..... 3-4 Manifold Module ..................................................................... ...................................................................................... ................. 3-4 Bottom Plate Assembly............................................................................ 3-6 Fluid Path................................................................................................. Path.................................................................................................3-6 3-6

Main Control Unit .................................................................... ..................................................................................... ................. 4-4 Driver Unit.................................................................. Unit ................................................................................................ .............................. 4-4

4.2

Keypad Board ......................................................................... ....................................................................................................... .............................. 4-4

4.3

Power Supply Supply Board Board ............................................................................ ............................................................................................. ................. 4-4

4.4

LCD Module................................................................ Module .......................................................................................................... .......................................... 4-5

4.5

Connecting Diagram ............................................................................ ............................................................................................. ................. 4-5

4.6

Interface Definition................................................................................................ 4-6

Maintenance and Service.....................................................................5-1 5.1

Maintenance ........................................................................... ......................................................................................................... .............................. 5-3 5.1.1 5.1.2 5.1.3

5.2

Service........................................................................ Service .................................................................................................................. .......................................... 5-4 5.2.1 5.2.2 5.2.3 5.2.4

5.3

Daily Maintenance .................................................................. ................................................................................... ................. 5-3 Weekly Maintenance .......................................................................... ............................................................................... ..... 5-3 Monthly Maintenance............................................................................... Maintenance...............................................................................5-3 5-3 Unclogging Needle ................................................................. .................................................................................. ................. 5-4 Replacing Tubing for Three-position Valve Valve.............................................. .............................................. 5-4 Replacing Tubing for Two-position Two-position Valve.................................................5-5 Valve................................................. 5-5 Troubleshooting ...................................................................... ....................................................................................... ................. 5-6

Software Upgrade................................................................... Upgrade ............................................................................................... ............................ 5-10

Contents

I

1

System Features

System Features

1-1

1-2

System Features

1 System Features

Specifications    

Dimension: 400 mm×330 mm×145 mm (L×D×H) Weight: about 7.2kg Power supply: 100-240V~, 50/60Hz Power: 100VA max

Cleaning Unit 





 

Dispense module: Two kinds of wash solution, Wash1 and Wash2; The maximum volume of each wash solution bottle is 2L; Solution is dispensed by a fluid pump; The dispense volume is 50-3000 µl; A three-position valve controls the switch between two kinds of wash solution; A two-position valve controls the fluid path of the dispense module. Waste module: Buffer and Waste bottles have been equipped with the system; Waste level can be detected; The maximum volume of each bottle is 2L; Solution is absorbed by a vacuum pump. Manifold: Separated dispense needles and aspirate needles; 8-/12-channel; Bump detection Tubing: Silicone tube used with valves must be replaced periodically. Connector: Easy to be connected

Moving Unit  

Vertical moving module: m odule: Drives the manifold to move m ove vertically. vertically. Horizontal moving module: Drives the plate carrier car rier to move horizontally.

Others   

The system is operated via the keypad on the front panel. Display: LCD, 192*64 Interface: RS-232

System Features

1-3

2

System Installation

System Installation

2-1

2-2

System Installation

2 System Installation 1

Ensure the installation field in the hospital is available. Refer to the Operation Manual for installation space, power supply, environmental requirements, etc.

2

Go to the installation site. Check the delivery list and unpack the package.

3

Move the system in place.

4

Install the manifold (8-channel or 12-channel) required by the user.

5

Connect Wash1, Wash2, Waste and Buffer bottles.

6

Connect the power cable and place the power switch to ON. The system will initialize and, after completing the self-check and rinsing tubing, display the main interface.

7

Place the power switch to OFF after ensuring the system can work properly. properly.

8

Train users. Can the user complete routine operations?

Yes

□

No □

Does the user know how to maintain the system?

Yes

□

No □

Does the user know how to clean the system?

Yes

□

No □

Does the user know how to correct common errors?

Yes

□

No □

Does the user know how to replace tubing for valves?

Yes

□

No □

System Installation

2-3

3

System Description

3.1

Workflow ..................................................................... ............................................................................................................... .......................................... 3-3

3.2

Components ........................................................................... ......................................................................................................... .............................. 3-3 3.2.1 3.2.2 3.2.3 3.2.4

Plate Carrier Assembly ....................................................................... ............................................................................ ..... 3-4 Manifold Module ..................................................................... ...................................................................................... ................. 3-4 Bottom Plate Assembly............................................................................ 3-6 Fluid Path................................................................................................. Path.................................................................................................3-6 3-6

System Description

3-1

3-2

System Description

3 System Description

3.1 Workflow A typical workflow of the system is shown as the figure below.

 S   t    a r   t  

I   n i    t   i    a l   i   z   a  t   i    o n

P   o  s  i    t   i    o n

P   a r   a m  e  t    e r   S   e  t    u

M i    c  r   o l    a  t    e W  a  s  h 

W  a  s  h   C   o m l    e  t    e  d 

All Operation Com let e

T   u  b  i   n

R  i   n  s   e

 S  h   u  t    d   o w n

1

Clear the Waste bottle. Add wash solution into Wash1 and Wash2 bottles. Connect the Wash1, Wash2, Waste and Buffer bottles to the system properly. Fill the Distilled Water bottle with distilled water or deionized water for use in washing.

2

Switch on the power supply and the system initializes. The initialization, which includes tubing rinse, self-check, mechanical parts reset, etc, takes about 20 seconds.

3

After the initialization, put the microplate on the plate carrier, select the manifold, and then adjust positions.

4

After adjusting positions, set parameters, such as program number, wash solution, wash mode, bottom wash, crosswise aspiration, soak time, shaking time, aspiration time, cycle times, etc.

5

Select a program, select pre-rinse as needed, and then set the row number. After that, start washing the microplate. m icroplate.

6

Select other microplate wash as needed.

7

After all wash operation is completed, disconnect Wash2 from the system, and connect Distilled Water bottle to the system through the connector which connects Wash2 and the system before. Run the tubing rinse program at least once. After that, shut down the system.

3.2 Components The system mainly consists of the plate carrier assembly, the manifold module, the bottom plate assembly and the fluid system.

System Description

3-3

3.2.1 Plate Carrier Assembly The plate carrier assembly holds microplates and moves them horizontally to wash position. It consists of the plate carrier, the guide shaft, the belt, etc.

The motor on the bottom plate, which drives the belt, is located near one end of the guide bar and the roller wheel near the other. The horizontal home position sensor, which is at the same end with the roller wheel, helps to control the reset of plate carrier in the horizontal direction. The spring sheet on the plate carrier secures the microplate to avoid shaking during the wash.

3.2.2 Manifold Module The manifold module supports the manifold and moves it vertically to reach specified positions. The module consists of the manifold assembly, the support assembly and the base assembly.

3.2.2.1 Manifold Assembly The manifold assembly has two types, 12-channel and 8-channel. The 12-channel is the default. Users can select appropriate manifolds according to their needs. Dispense and waste connectors on one side of the manifold connect dispense tubing and waste tubing respectively. Screws on the other side secure the manifold to the support assembly and form the bump detection circuit.

3-4

System Description

3.2.2.2 Support Assembly The support assembly, which consists of a metal plate and a non-metal one, supports the manifold assembly. The metal plate is the frame and the non-metal one provides insulation for the bump detection circuit. The conducting wire between the two plates is connected to the two electrodes fixed on the non-metal plate and forms the bump detection circuit with the screws on the manifold and the screw connection sheet.

3.2.2.3 Base Assembly The base assembly serves as the base of the manifold module, fixes the driving motor, and guides and locates the sliding block. The vertical home position sensor on the top of the base assembly detects the vertical moving status of manifold and helps to control the manifold reset.

System Description

3-5

3.2.3 B Bottom ottom Plate Assembly The bottom plate assembly supports other assemblies, parts, etc. The vacuum pump, fluid pump, valves, power supply unit, main control board and motors are located on the bottom plate. Power supply input interface and fluid path connectors are located on the rear panel.

3.2.4 Fluid Fluid Path The fluid path dispenses wash solution and collects waste. Two kinds of wash solution are supported.

3-6

System Description

3.2.4.1 Wash Solution Dispensing The wash solution dispensing path consists of the fluid pump, three-position valve, two-position valve, wash solution bottles and related tubing and connectors. The fluid pump provides power for dispensing. The three-position valve performs the switch between the two kinds of wash solution. The two-position valve can shut off the path to avoid solution leakage. Wash1 and Wash2 bottles provide wash solution, which should be added as necessary. necessary. During the wash, the wash solution is powered by the fluid pump and goes from wash solution bottles to needles through the dispensing tubing and finally reaches wells.

3.2.4.2 Waste Collection The waste collection path is powered by the vacuum pump to collect waste. The Waste and Buffer bottles work together to collect waste into the Waste bottle and to prevent waste from entering the vacuum pump. During the collection, the manifold moves downward and absorb needles suck waste near the bottom of wells.

System Description

3-7

4 4.1

Hardware Boards

Main Control Board............................................................................................... 4-3 4.1.1 4.1.2

Main Control Unit .................................................................... ..................................................................................... ................. 4-4 Driver Unit.................................................................. Unit ................................................................................................ .............................. 4-4

4.2

Keypad Board ......................................................................... ....................................................................................................... .............................. 4-4

4.3

Power Supply Supply Board Board ............................................................................ ............................................................................................. ................. 4-4

4.4

LCD Module................................................................ Module .......................................................................................................... .......................................... 4-5

4.5

Connecting Diagram ............................................................................ ............................................................................................. ................. 4-5

4.6

Interface Definition................................................................................................ 4-6

Hardware Boards

4-1

4-2

Hardware Boards

4 Hardware Boards The hardware system consists of the following units and boards:     

Main control unit: main control board Driver unit: main control board Keypad unit: keypad board Display unit: LCD module Power supply unit: power supply board Hardware System

Main Control Unit

Driver Unit

Main Control Board

Power Supply Unit

Display Unit

Keypad Un Unit

Power Supply Board

LCD Module

Keypad Board

4.1 Main Control Board Functions of the main control board is shown as the figure below. Power Supply Board + 24V/ +12V/ + 5V

Power Supply

Main Control Board Moving Parts Driver

Driver of 2 Step Motors

Step Motor Interface

Signal from 2 Position Sensors

Position Sensor Interface Keypad Interface

Motors and Position Sensors

Pump and Valve Driver

Main Control Unit

Keypad

Pump and Valve Interface Waste Level Sensor Interface

Driver of 2 Pumps and 2 Valves Signal from Waste Level Sensor

LCD LCD Interf Interface ace Serial Serial Port Port

LC D Module

PC

Hardware Boards

Pumps, Valves, Waste Level Sensor

4-3

4.1.1 Main Control Unit The main control unit uses a C8051 SCM and a CPLD to control and operate all objects. The unit performs the following functions: 



  







Communication with PC. The main control board communicates with PC through RS232 port, which is for software upgrade. User parameters storage. The board uses EEPROM to store parameters. The operation is performed through SPI interface. 8k bytes or 100 user-defined programs can be stored. Motor control. The CPU controls motors directly. Valve and pump control. The CPU controls pumps and valves directly. directly. Sensor signal detection. The CPLD detects signals from sensors and the software reads sensor status. The sensors are the vertical home position sensor, the horizontal home position sensor, the waste level sensor and the manifold bump detection sensor. LCD display control. The CPLD receives control and display data through the extended CPU interface and then converts it into the data that conforms to the LCD sequence. The CPLD also reads LCD status. Keypad scanner. The 2*4 matrix keypad is controlled by the CPLD. When a key is pressed, the CPLD detects it and produces a CPU interrupt to instruct the CPU to read the key value. Two or more keys pressed simultaneously cannot be supported. Buzzer control. The CPLD converts instructions from CPU to signals that control the buzzer.

4.1.2 D Driver river Unit The driver unit drives: 



Two step motors, which drives the manifold to move vertically and the plate carrier to move horizontally. The motor driver uses two A3979 chips and adopts 16-subdivision drive to improve stability of motors and to decrease noise when motors work at low speed. One chip drives one motor. The chip receives direction, speed, current control and enable signals and outputs signals to drive the motor. Two pumps and two valves, which includes an air pump, a fluid pump, a three-position valve and a two-position valve. The pump and valve driver uses IRF3710 MOSFET to switch.

4.2 Keypad Board The keys are on the keypad board and are installed into the system with the board.

4.3 Power Supply Board The power supply board outputs: 

+5V: 0.6A, which is supplied to the boards;



+12V: 4.0A (peak current), 2.0A (rated current)，which is supplied to pumps and valves; +24V: 0.3A, which is supplied to step motors; m otors;



4-4

Hardware Boards



-12V: reserved.

The power supply board consists of:     

EMI filter circuit; Rectification and filter circuit; Flyback converter, which outputs +12V, +24V and -12V; Voltage reducing circuit, which generates generat es +5V from the +24V +2 4V input; Overcurrent and overvoltage protection circuits for outputs.

4.4 LCD Module The LCD module, which is a standard low-voltage graphic dot-matrix one, has a LCD controller KS0108 and an integrated DDRAM. The module is operated by specific control sequences and instructions.

4.5 Connecting Diagram

LCD Module

2 Position Sensors

Vocuum Pump Fluid Pump Three-position Valve

Two-position Valve

AC

Power P1 Supply P2 Board

APUMP J5 J6 WPUMP J19 VALVE1 J18 VALVE2

LCD J11

PHO J13

Main Control Unit

J4 CRASH

J12 WASTE

J7 KEYBOARD

J1      R      E      W      O      P HMOTOR

J3

VMOTOR J2

LED J15

Step Motor (Horizontal)

Step Motor (Vertical)

LED

Hardware Boards

J8 COM

Bump Detection Sensor

Waste Level Sensor

J1 Keypad Board

Serial Port

4-5

4.6 Interface Definition Threeposition Valve +

Two-position Valve +

Power Supply Board

Fluid Pump +

GND GND GND

VALVE2

2  4   V

1  2   V

 5   V

PWR J1

BB+ A-

WPUMP

J19 PIN 1

J6 PIN 1

PIN 1

P  I    N 1 

 J   2 

H M  O T   O R

VALVE1

J18

 J    5 

P  I    N 1 

A P   U M P 

NC

V  - P  a  u  c  m  u  u  +  p m

PIN 1

A+

LCD_LED+ GND LCD_RST LCD_CS3 LCD_CS2 LCD_CS1 LCD_EN LCD_RW LCD_RS L  GND  C  D  GND M LCD_D7  o  d  LCD_D6  u l   LCD_D5  e LCD_D4 LCD_D3 LCD_D2

 J   1  1   J    3 

B-

 V M  O T   O R

B+ A-

PIN 1

A+

L   C  D

M  a i   n  C   o n  t   r   o l   B   o  a r   d 

LCD_D1 GND LCD_D0 LCD_VEE LCD_VO VCC +5V GND

PIN 1

VPHO GND

GND LED+

 J   1   5 

L  E  D

 J   1   3 

P  H  O

+5V HPHO

PIN 1

GND PIN 1

P  I    N 1 

P  I    N 1 

P  I    N 1 

J8

J7

P  I    N 1 

J12

KEYBOARD COM

+5V

J4

WASTE

CRASH

L  E   V E  L 

L  E   V 1 

 N  N  C  C 

Serial Port

PC

4-6

K   0   a n  d  K  1  n  o  t    c   o n n  e  c   t    e  d 

R  0 

 C   0 

R 1 

 C  2 

 C  1 

 C  4 

 C   3 

 G  N D

Waste Level Sensor

 G  N D

Bump Detection Sensor

 C   5 

Keypad Board

Hardware Boards

 J   1  4 

P  V   S  o  e  s   e r  n i    t    s   t   i   i    c   o  o r  n  a l   H  P  o  S  o r   e  s  i   z  n i    o  s   t   i   n  o  o r  n  t    a l  

     0     1     0     1     2     3     4      R     R     C     C     C     C     C

J1

Keypad Board

In the figure above, R0 means ROW0 (the row wire) and C0 means COL0 (the column wire).

1

GND VCC_+5V LCD_VO LCD_VEE LCD_RS LCD_RW LCD_EN LCD_D0 LCD_D1 LCD_D2 LCD_D3 LCD_D4 LCD_D5 LCD_d6 LCD_D7 LCD_RST LCD_CS1 LCD_CS2 LCD_CS3 LCD_LED+

LCD Module

NC 21

Power Supply Board P1

P2

     1      N      I      P

     1      N      I      P

 N  C   N

L 

+ +  G 1   G 2  +  N  G 1  2   G 2   N 4   5   N  N  V D  V  V  V D D D

AC Main Control Board PWR J1

Hardware Boards

4-7

5 5.1

Maintenance and Service

Maintenance ........................................................................... ......................................................................................................... .............................. 5-3 5.1.1 5.1.2 5.1.3

5.2

Service........................................................................ Service .................................................................................................................. .......................................... 5-4 5.2.1 5.2.2 5.2.3 5.2.4

5.3

Daily Maintenance .................................................................. ................................................................................... ................. 5-3 Weekly Maintenance .......................................................................... ............................................................................... ..... 5-3 Monthly Maintenance............................................................................... Maintenance...............................................................................5-3 5-3 Unclogging Needle ................................................................. .................................................................................. ................. 5-4 Replacing Tubing for Three-position Valve Valve.............................................. .............................................. 5-4 Replacing Tubing for Two-position Two-position Valve.................................................5-5 Valve................................................. 5-5 Troubleshooting ...................................................................... ....................................................................................... ................. 5-6

Software Upgrade................................................................... Upgrade ............................................................................................... ............................ 5-10

Maintenance and Service

5-1

5-2

Maintenance and Service

5 Maintenance and Service

5.1 Maintenance 5.1.1 Daily Maintenance The following maintenance can be performed by users. Clean the liquid splashed on the surface of the system. Perform the tubing rinse procedure before shutting down the system. Perform routine rinse for tubing with distilled water or deionized water.

5.1.2 Weekly Weekly Maintenance The following maintenance can be performed by users. Slightly pull the tubing connected to valves to move the tubing. Use ethyl alcohol (50%) to clean the retaining screws on the manifold and the metal electrodes on the manifold support.

5.1.3 Monthly Monthly Maintenance The following maintenance can be performed by users. Use ethyl alcohol (50%) to clean:   

The manifold support. The plate carrier and the spring sheet on the carrier. The dispense and absorb needles.

Use warm water (about 40°C) to wash the Wash1, Wash 2, Waste, Buffer and distilled water bottles.

Maintenance and Service

5-3

5.2 Service 5.2.1 Unclogging Unclogging Needle Ne edle During the cleaning, if a dispense needle injects little, or a absorb needle sucks little, or the residue is much after the absorbing, the needle may be clogged. You can unclog the needle as follows: 1

Find out which needle is clogged.

2

Slightly hold the manifold and make the needle face up. Use a stainless needle whose diameter is about 0.5mm to unclog the needle.

3

After the unclogging is completed, reassemble the manifold onto the system and run the rinse procedure to observe if the needle can work properly.

5.2.2 Replacing Tubing for Three-position Valve The tubing connected to the three-position valve may age or be damaged after being used for a long time. tim e. You You can replace it as follows:

5-4

1

Power off the system and disconnect the power cable.

2

Remove the screws on the rear panel and the bottom of the system. Open the upper cover and place it on the right of the system.

Maintenance and Service

3

Disconnect the tubing that connected to the three-position valve from the Y-type connector and the Wash 1, Wash 2 connectors. Take out the tubing connected to the outer channel of the valve first. Then slightly press the black button on the end of the valve, and take out the tubing connected to the inner channel of the valve and then release the button.

4

Cut two segments of new tubing. Ensure they are as long as the old ones. Connect respectively the two segments of tubing to the Wash 1 and Wash 2 connectors on the inside of the rear panel. Then make them pass through the holes in the rear panel. Press slightly the black button on the end of the valve, connect the tubing connected to the Wash 2 connector to the inside channel of the valve, and then release the button and connect the other segment of tubing to the outer channel of the valve.

5

Put the two segments of tubing through their own installation holes on the rear panel. Then connect them to the two mouths of the Y-type connector.

6

Reassemble the upper cover. Connect the power cable and turn on the system. Put an used microplate on the plate carrier and then perform the rinse procedure respectively with Wash 1 and Wash 2. Observe the dispense needles to judge if the tubing can work properly. properly.

5.2.3 Replacing Tubing for Two-position wo-pos ition Valve The tubing connected to the two-position valve may age or be damaged after being used for a long time. You You can replace it as follows: 1

Power off the system and disconnect the power cable.

2

Open the upper cover and place it on the right of the system.

3

Disconnect the tubing from the connectors. Use a hex wrench (diameter = 1.5mm) to slightly press the press head of the valve, and pull out the tubing. Release the head.

Maintenance and Service

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4

Cut a segment of new tubing. Ensure it is 20mm longer than the old one. Cut one end to the diagonal shape (about 20mm long) shown as the figure below.

5

Connect the flat end of the new tubing to the bigger connector inside the system. Pass the tubing through the corresponding installation hole in the rear panel.

6

Use a hex wrench (diameter = 1.5mm) to slightly press the press head of the valve and pull the diagonal end of the tubing through the hole in the valve. After pulling the tubing in place, release the press head.

7

Cut the diagonal end (about 20mm long) of the tubing. Pull the tubing through the installation hole in the rear panel and connect it to its corresponding connector.

8

Reassemble the upper cover. Connect the power cable and turn on the system. Run the tubing rinse procedure. Observe the dispense needles to  judge if the tubing can work properly.

BIOHAZARD Wear gloves and lab coat and, if necessary, goggles. In case your skin contacts the waste, follow standard laboratory safety procedure and consult a doctor.

NOTE When pulling the tubing through a hole, exercise caution to prevent it from being damaged.

5.2.4 Trouble Troubleshooting shooting 5.2.4.1 Manifold Failure

5-6

Maintenance and Service

If the check mode is selected, when the connection of the manifold is contaminated or the manifold is not in the right position, the following information will be displayed during washing：

Error! Please check the manifold ! Press OK to continue or EXIT to cancel ! You can correct the problem as follows: 1

Check if the manifold is placed correctly and not tilted, place it level if needed. Press OK and check if the system works normally; or

2

Lift up the manifold, then wipe the retaining screws and metal plates with clean cotton cloth dipped with ethanol. Install the manifold properly and press OK; or

3

Restart the system and run the wash program.

4

If things like removing manifold during wash do not happen, in the meantime standard micro plate is used with no conspicuous dimension difference between the manifold and the micro plate, the default mode—No check（

）can be used.

5.2.4.2 Movement Error During the startup or movements, if a movement error occurs, the system will warn and display the following information:

Error! XXXX movement error ! Restart or see the user manual ! In the information above, “XX” represents “horizontal” or “vertical”. You can correct the error as follows:

Maintenance and Service

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1

Switch off the power and restart the system; or

2

Switch off the power. In case of horizontal movement error, move the plate carrier back and forth for 5 times. In case of vertical movement error, move the manifold up and down for 5 times. Then restart the system and check if it works normally. normally.

5.2.4.3 Dispense Needle Error During the wash, if a dispense needle injects little or no wash solution, you can do as follows: 1

Check if the wash bottle is connected to the washer correctly. correctly. Reconnect the tubing if needed; or

2

Check if the fluidic tubing is creased, stretch it if necessary; or

3

The fluidic tubing may adhere to each other if not moved for a long time. As for tubing clamped by the three-position valve, first pull the outer tubing a little, then keep pressing the black end of the three-position valve and pull the inner tubing. As for tubing clamped by two-position valve, first run a rinse procedure for about 20s, then pull the tubing quickly when the dispense needle is dispensing liquid; or

4

Check if the tubing end inside the wash bottle is blocked. If it is, remove the bottle cap and then unclog the tubing; or

5

Check if the manifold inlet and dispense needles are clogged. If they are, remove the foreign matters from the manifold inlet using tweezers, and use the supplied stainless needle to unclog the blocked dispense needles (see the section “5.2.1 Unclogging Needle”); or

6

Interchange the dispensing tubing and the waste tubing, then press MAINT to backflush the dispensing tubing with air flow, drawing the foreign matters into the waste bottle. Reconnect the tubing correctly and run a rinse procedure again.

5.2.4.4 Residue Too Too Much When excessive residue exists in microwell during washing process, you can correct the error as follows: 1

5-8

Check if the waste/buffer bottle caps are tightened or sealed, or the fluidic tubing is connected properly to the bottle cap. Tighten the bottle caps and reconnect the fluidic tubing to the bottles; or

Maintenance and Service

2

Check if the distance between aspirate needle and microwell bottom is too large and not within 0.1-0.4mm. 0. 1-0.4mm. You’re You’re recommended to set the bottom wash height using the Bot Auto Ht function.

3

Check if the fluidic tubing is creased, stretch it if necessary; or

4

Check if the tubing end inside the waste waste or buffer bottle is blocked. If it is, remove the bottle cap and then unclog the tubing; or

5

Check if the manifold inlet and aspirate needles are clogged. If they are, remove the foreign matters from the manifold inlet using tweezers, and use the supplied stainless needle to unclog the blocked aspirate needles; or

6

Interchange the aspiration tubing and the waste tubing, then press MAINT to backflush the waste tubing with distilled water. Reconnect the tubing correctly and run a rinse procedure again.

7

If the manifold has not been used for a long time, the connector might get rusty, leading to the change or lifting of the connector. Follow the 5.1.2 to clean the connector of the manifold. If the rust is very serious, use abrasive paper of small granule to rub the rust away from the manifold retaining screw.

5.2.4.5 Wash Solution Overflow If the dispense needles dispense excessive wash solution into the microplate wells, resulting in overflow, you can correct the error using the following methods: 1

Check if the waste/buffer bottle caps are tightened or sealed, or the fluidic tubing is connected properly to the bottle cap. Tighten the bottle caps and reconnect the fluidic tubing to the bottles; or

2

Check if the fluidic tubing is creased, stretch it if necessary; or

3

Check if the aspirate needles are far from the well. Check the dispensing height and adjust the Disp Pos correctly; or

4

Check if foreign matters exist at two-position valve on the rear panel of the washer, disabling the valve core to clamp the tubing tightly. Remove the tubing clamped by the two-position valve and remove the foreign matters.

5.2.4.6 Collision of Manifold and and Microplate Microplate If the manifold collides with the microplate, causing the manifold to fall off or the plate is tilted, you can handle the error using the following methods: 1

If the microplate is not placed properly on the carrier, reset the plate and attach it to the carrier surface completely; or

2

Check the manifold position is set properly. properly. Reset the crosswise aspiration positions (Left Pos and Right Pos), ensuring the aspirate needles lie inside the wells. Also check if the dispensing height is set correctly; c orrectly; or

3

Check if the plate carrier or manifold support is blocked. Remove the foreign matters if any. any.

Maintenance and Service

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NOTE The service life of consumables like plastic bottles, connectors or manifold is about 24 months and that of tubing is about 18 months.

5.3 Software Upgrade The system uses the serial port to communicate with a PC to upgrade and maintain the software. The serial port locates on the rear panel of the system. The upgrading procedure is as follows: 1

Use serial port cable to connect the PC and the MW-12A. Start the system and the PC. Open the Download software （P/N:110-000208-00）on PC.

2

Select the parameter as the following figure. Figure 5-1 Software upgrading

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3

Select the file folder where the upgrading program is and open it, click Start .

4

After upgrading is completed, click Exit  click Exit , disconnect the serial port cable.

Maintenance and Service

P/N: 0105-20-63177 (3.0)