MultiGene Thermal Cycler TC9600-G/ TC9600/TC6000-G/TC6000
Service Manual Ver.1.0
PCR Disclaimer:
MultiGene Thermal Cyclers can be used for a variety of molecular biology applications and techniques, including the Polymerase Chain Reaction (PCR). PCR and other molecular biology processes and techniques may be protected by patents.
Purchase of
this product does not convey a license or right to perform patented PCR processes. Applied Biosystems holds some of the basic PCR patents that may or may not be currently valid.
For additional additional information information on PCR patents held by Applied Biosystems
and information about acquiring any required licensing for using PCR techniques, please contact Applied Biosystems at 800-327-3002 or 650-638-5800 or their website: www.appliedbiosystems.com . www.appliedbiosystems.com .
TABLE OF CONTENTS
1
SAFETY PRECAUTIONS......... PRECAUTIONS ................... .................... ................... ................... .................... ..................1 ........1
2
SYSTEM DESCRIPTION ..................... ............................... .................... .................... .................... ...............2 .....2 2.1 DESIGN CONCEPT ........................... ........................................ ........................... ........................... ........................... ................... .....2 2 2.2 ELECTRIC SPECIFICATIONS.......................... ....................................... .......................... ........................... ................... .....4 4 2.3 PCBS DESCRIPTION .......................... ........................................ ........................... ........................... ........................... ................ ... 5 2.4 WIRE CONNECTION DIAGRAM ........................... ......................................... ........................... .........................6 ............6 2.5 PCBS’ CONNECTOR LOCATIONS ........................... ........................................ .......................... ....................... ..........7 7
3
SERVICE MODE...............................................................................10 3.1 DIAGNOSTIC ........................... ......................................... ............................ ............................ ........................... ........................ ...........10 10 3.2 LID CALIBRATION ............................ ......................................... ........................... ............................ ............................ ................ 10 3.3 BLOCK CALIBRATION .......................... ........................................ ............................ ........................... ........................ ...........13 13
4
SOFTWARE SOFTWARE UPGRADE ................... ............................. .................... .................... .................... .................17 .......17 4.1 INSTALL THE SOFTWARE........................... ........................................ ........................... ............................ .................... ......17 17 4.2 INSTALL THE HARDWARE ........................... ......................................... ........................... ........................... .................. .... 17 EFLASH THE NEW SOFTWARE ........................... 4.3 R EFLASH ......................................... ........................... ...................... .........18 18
5
TROUBLESHOOTING .................... .............................. .................... .................... .................... ..................20 ........20 5.1 ERROR MESSAGES .......................... ........................................ ............................ ............................ ............................ ................ 21 5.2 TROUBLESHOOTING FLOWS ........................... ......................................... ............................ ...........................22 .............22
6
5.2.1
Beeper has no sound ............................................. .................................................................... .....................................22 ..............22
5.2.2
LCM has no display ............................................ .................................................................... .......................................22 ...............22
5.2.3
Buttons are not activated ............................................ .................................................................... ...............................24 .......24
5.2.4
TE Module and PT-1000 Checkup .............................................. .............................................................25 ...............25
DISASSEMBLY/ASSEMBLY...........................................................26 6.1 FRONT PANEL DISASSEMBLY .......................... ........................................ ............................ ...........................26 .............26 6.2 SAMPLE BLOCK DISASSEMBLY ........................... ......................................... ........................... ...................... .........27 27 6.3 TOP CASE DISASSEMBLY ............................ .......................................... ........................... ........................... .................. .... 28 6.4 LID MODULE DISASSEMBLY ........................... ......................................... ............................ ...........................30 .............30 NOB DISASSEMBLY ............................ 6.5 LID K NOB .......................................... ........................... ........................... .................. .... 32
6.6 MCU CIRCUIT BOARD DISASSEMBLY ....................................................34 6.7 POWER CIRCUIT BOARD DISASSEMBLY .................................................35 6.8 SYSTEM FAN DISASSEMBLY .......................... ........................................ ............................ ........................... ............... .. 38
7
SPARE SPARE PARTS PARTS LIST AND EXPLODED DIAGRAM ...................38 ...................38 7.1 FRONT PANEL ASSEMBLY ........................... ......................................... ........................... ........................... .................. .... 39
7.2 LID ASSEMBLY ........................... ......................................... ............................ ........................... ........................... ..................... ....... 40 7.3 TOP CASE ASSEMBLY.......................... ........................................ ............................ ........................... ......................... ............ 42 7.4 BOTTOM CASE ASSEMBLY .......................... ........................................ ............................ ............................ ................ .. 44 7.5 FAN ASSEMBLY........................... ......................................... ............................ ........................... ........................... ..................... ....... 46 7.6 COMPLETE ASSEMBLY ............................ .......................................... ........................... ........................... ..................... ....... 47
MultiGene Service Manual
1 Safety Precautions Before servicing the MultiGene thermal cycler for the first time, please read this entire service manual carefully. The information contained herein is provided to give an understanding of the workings of the MultiGene. All service work must be carried out by authorized service personnel. Labnet will not be responsible for injuries and or damages caused as a result of unqualified or unauthorized persons performing service. All necessary safeguards below must be taken when working on equipment operating from the mains power supply. supply.
Disconnect power supply before opening the machine or performing service on the machine.
Only use original accessories and spare parts provided by Labnet.
To avoid burns, service the machine after the heating lid and block cool down.
Work at an earthed bench with wrist straps and earthed flooring.
This manual is not subject to the update product revision. If you find the repair unit’s designs or components are different from those described in this Service Manual, please ask Labnet’s Service Department for a current Service Manual version. Please remember to record the serial no. and software version before calling or e-mail Labnet’s Service Department for assistance.
High Temperature Label: Please be aware of the heated block. Warning Label: Please be aware of the danger of electric shock or other dangers.
If you need additional assistance while using this service manual, please contact Labnet’s Service Department for assistance.
1
2 System Description The MultiGene Gradient thermal cycler is a powerful, reliable and affordable thermal cycler. It is equipped with a gradient function that enables the user to set a maximum 24 C temperature gradient between the left and right rows in the sample block at each °
temperature. For product specifications, feature and operation procedures, please refer to the “MultiGene Thermal Cycler Operation Manual” for more information.
2.1 Design Concept Thermoelectric Module The MultiGene thermal cycler is a Peltier-based device. Peltier(Thermoelectric
or TE) is used for heating and cooling of the sample block. A powerful Microprocessor(MCU) is used to accurately control the system to reach a desired temperature rapidly and stay for a set period of time. Users can use this unit’s user-interface to program and store their methods for future usages. The key components of the Sample Block are are shown in Figure 1. The key components’ functions are described des cribed below.
Lid Heater Module : is used to create high temperature (60~65 °C or 100 ~ 115 °C )
on the top of plastic tube to prevent the condensation of water.
Tube : Sample storage
Block Module: tube holder and temperature conduction component. The block
module is Aluminum.
Thermoelectric Module (Peltier) : heating and cooling component
Heatsink : Heat dissipation component
Fan : to increase airflow circulation and heat dissipation
2
MultiGene Service Manual
Lid Heater Tube Block Module Thermoelectric Module Heatsink
Fan Figure 1. Design of Sample Block
The design block diagram of MultiGene is shown in Figure 2. The temperature control of Lid Heater Module uses PWM voltage control. The lid temperature is fed back to MCU by a temperature sensor: NTC(Negative Temperature Coefficient ), while the Block Module uses three more accurate temperature sensors: PT-1000Ω. Both the Lid Heater Module and 96-well Sample Block have a Thermal Cutoff Fuse which is used to protect the unit from overheating. When the MCU is abnormal or electronic components are damaged, the Lid Heater Module and Thermoelectric Module can’t be controlled and continue to heat up. To To prevent the unit from damage, the Thermal Cutoff Fuse will fail when the temperature exceeds specification. Other key components of the System and their functions are described below.
Fan: is used to speed up the heat dissipation of Block module. The speed of fan is
controlled by MCU by a Constant Voltage method.
Key Pad: is used to key in the desired temperature, time and other settings.
Liquid Crystal Module (LCM): is used to display the protocols and settings.
Real Time Clock (RTC): is used to generate date and time info.
EEPROM: is used to store the protocols and settings.
Power Supply: is used to provide the unit the required power. Input voltage: AC100
~ 230V. Output voltage: DC +24V. Max. Output power: 500VA. Note: Please use 7A fuse in power source 100~120/50~60Hz countries, while 3.15A fuse
3
is for power source 200~220V/50~60Hz countries.
Figure 2. Design block diagram
2.2 Electric Specifications Parts Name Thermoelectric
Specifications
6 units, Max. Ma x. Current: 6A, Max. Voltage: Voltage: 17.5V
Module(TE) PT-1000(Block
3 units, Platinum, 1000 Ω at 0ºC
Temperature Sensor) Thermal cut-off fuse
One on Sample Block, and one on Lid Heater(Max. 157ºC)
Fan
DC24V, 0.8A, 19.2W
LCM
240 x 120 pixels, backlight adjustable
Battery
Lithium, 3V, 225mAh
Lid Heater
Silicon rubber, DC24V, Max. Current: 2.5A
NTC(Lid Heater Sensor)
Range: -40~250ºC; 100K Ω at 25ºC
Power Supply
Switching; Input: AC85V-260V, Output: DC24V, 20A; 500W
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MultiGene Service Manual
2.3 PCBs Description Parts Name
Part No.
Function
Power Board PCB
TC96005-RP
Provide DC voltages to Peltier, Fan, Lid Heater and other components.
MCU Board PCB
TC96006-RP
Control heating and cooling of Sample Block and Lid Heater Module and Others.
RS-232 PCB
193-pcr150-00
RS-232
port,
USB
brightness control.
5
port
and
LCD
2.4 Wire Connection Diagram The wire connection diagram is shown below. Users can use this diagram to understand the connection between the wires and the circuit boards. RS232 Board
LCM module
Keypad module
(P/N:193-pcr150-00)
(P/N:156-07p010-c1)
(P/N: 215-000001-00)
CN501
150-p01c10-05
56-07p010-c1
CN103
CN102
215-000001-00
CN105
CN101
MCU Board (P/N:TC96006-RP) CN108
CN107
150-p01c10-06
150-p01c10-07
CN301
CN104
CN106
CN302
150-p01c10-04
MSP430 FET
CN309
Power Board (P/N:TC96005-RP) CN310
CN308
CN306
CN303
CN305 CN304 CN307
150-p01c10-03
150-p01c10-02
275-heater-00
123-12412h-f1
Power Supply
Slide cover assembly
Fan module
Block module
(P/N:TC96007-RP)
(P/N:TC96012-RP)
(P/N:TC96004-RP)
(P/N:TC96008-RP)
150-p01c10-01
Power Switch (P/N:122-42608r-00)
Figure 3. Wire connection diagram 6
MultiGene Service Manual
2.5 PCBs’ Connector Locations Power Board PCB Connector
Link
Wire
Application
part number CN301
MCU Board CN107
150-p01c10-07
Controls
Signal
from
MCU
Board. CN302
MCU Board CN108
150-p01c10-06
Temperature control Signal to MCU Board.
CN303
Slide cover assembly
275-heater-00
( Heater module )
Temperature control Signal from Slide cover assy. as sy.
CN305
Fan module
123-12412h-f1
Supplies power to Fan module.
CN306
Slide cover assembly
275-heater-00
Supplies
( Heater module ) CN308
Power Supply
power
to
Heater
from
Power
module. 150-p01c10-03
Supplies
power
Supply. CN309
MCU Board CN106
150-p01c10-04
Supplies power to MCU Board.
CN310
Power Supply
150-p01c10-02
Controls power supply ON/OFF of Block module.
CN301
CN309 CN302
7
CN308
CN310 CN306 Figure 4. Connectors of Power Board
CN303 CN305
MCU Board PCB Connector
Link
Wire
Application
part number
CN101
Front panel assembly
215-000001-00
( Keypad module ) CN102 CN103
RS232 CN501
Front panel assembly
Keypad control signal to MCU Board.
150-p01c10-05 156-07p010-c1
Control signal to RS232 Board.
LCM backlight adjustment.
LCM control signal from MCU
( LCM module )
Board.
CN104
MSP430 FET
----------
For firmware update only.
CN105
Front panel assembly
156-07p010-c1
LCM
( LCM module )
CN106
power
from
MCU Board.
150-p01c10-04
Power Board
backlight
Supplies
power
from
Power
Board.
CN107
Power Board CN301
150-p01c10-07
Control Signal to Power Board.
CN108
Power Board CN302
150-p01c10-06
Temperature Control Signal from Power Board.
CN107 CN106
CN102 CN104
CN108
Pin 1 CN101
CN103
Pin 1
CN105
Figure 5. Connectors of MCU Board PCB 8
MultiGene Service Manual
RS232 Board PCB Connector
Link
Wire
Application
part number
CN501
MCU Board CN102
150-p01c10-05
Controls
signal
from
Board.
CN501
9
LCM backlight adjustment.
Figure 6. Connectors of RS-232 Board PCB
MCU
3 Service Mode In Service Mode, users can perform: 1) Diagnostic function to check the heating rate and cooling rate of Sample Block, 2) Calibration Lid: to calibrate the lid heater’s temperature, 3) Calibration Block: to calibrate the 3 different Peltier module set points(Right, Center and Left). To enter Service Mode, please choose Service Mode under SYSTEM function option. The password is “8888”.
3.1 Diagnostic After entering Service Mode, choose the Diagnostic function to check out the heating rate and cooling rate of sample block. Press F1:START to start the heating and cooling rate check. The result will be shown as below screen.
SERVI CE>DI AGNOS TI CS 1 T Tem p
1 T Tem p
2007/ 01/ 05 10: 30 1 T Tem p
Temp
20.0
94.0
4.0
T m i e m
0:30
0:30
0:30
Heat r at e: 03. 2 / S
,
Cool r at e: 02. 3 / S EXI T
F1
F2
F3
F4
F5
Figure 7. Heating/Cooling rate screen
If the heating rate is slower than 3°C /s or the cooling rate is slower than 2°C, calibration may be necessary. nece ssary.
3.2 Lid Calibration To calibrate the temperature of lid heater, a calibrated thermometer equivalent to Dual Channel Thermometer ( Cat. No.: 23609-230 ) from VWR ( http://www.vwr.com
) or
TM-363 from TENMARS ( http://www.tenmars.com/front/bin/home.phtml http://www.tenmars.com/front/bin/home.phtml ) ) and a K-type
10
MultiGene Service Manual
thermocouple temperature probe are required. Pierce the flat cap of a 0.2ml plastic tube and insert the thermocouple temperature probe as shown in figure below. Place the plastic tube with thermocouple temperature probe into well D6 of the sample block. Place 4 empty tubes at each corner of the block and close the lid well D6
Figure 8. Lid temperature probe location
Select “Lid Calibration” under Service Mode, the screen will display as below
CAL I BRA TI ON>L I D
2007/ 1/ 5 10: 50
H T105
+0. 3
060. 0
H T60
+0. 0
060. 0
ROOM
+0. 0
024. 0
T me:
:
H T Cal . L d
Timer
Calibration Value Measured
H T60
F1
H T105
F2
+
-
F3
F4
HO T! T! Exi t
Value
F5
. Figure 9. Lid Calibration screen
We use a two-point(105 °C and 60°C) curve method to calibrate the temperature curve of lid heater.
11
Another feature of this mode is the ability to calibrate the Room Temperature which is shown as “ROOM” in the Figure 9 above. If the room temperature is too high( above 30°C ), the unit will show an error message “ROOM > 30 °C” and terminate the protocol. Please follow the procedures below to calibrate the lid temperatures and room temperature. A.
Lid temperature temperature calibration at 105
Press F2: HT105
. The temperature of lid will go up to 105 °C.
When the timer shown in the right-hand side of Figure 9 reaches five minutes ( Time: 05:00 ), record the readout of the Thermometer.
Using the formula below to calculate the Offset temperature value: Offset Value = Thermometer Readout - Measured Value (by lid’s
temperature sensor)
Input the sum of factory-calibrated Calibration Value and Offset Value into HT105°C’s Calibration Value column to calibrate the lid temperature. Press F3 or F4 to change the Calibration Value to minus or plus. The calibration procedure might need to be repeated repea ted several sever al times ti mes to t o make the Offset Value under 1°C.
B.
Calibration is complete once the Offset Value is less than 1°C. Lid temperature temperature calibration at 60
Press F1: HT60
. The temperature of lid will go up to 60
.
When the timer reaches five minutes ( Time:05:00 ), record the readout of the Thermometer.
Using the formula below to calculate the Offset temperature value: Offset Value = Thermometer Readout - Measured Value (by lid’s
temperature sensor)
Input the sum of factory-calibrated Calibration Value and Offset Value into HT60 °C’s Calibration Value column to calibrate the lid temperature. Press F3 or F4 to change the Calibration Value to minus or plus. The calibration procedure might need to be repeated repea ted several sever al times ti mes to t o make the Offset Value smaller than 1 °C.
C.
Calibration is complete once the Offset Value is less than 1°C. Adjusting ROOM temperature
Place the temperature probe of a thermometer in the front vent as shown in Figure 10. Move cursor to Room’s Room’s Calibration Value column.
12
MultiGene Service Manual
Using the formula below to calculate the Offset temperature value: Offset value = Thermometer readout - Measured value (by temperature
sensor on MCU board)
Input the sum of factory-calibrated Calibration Value and Offset Value into Room’s Calibration Value column to calibrate the Room temperature. Press F3 or F4 to change the Calibration Value to minus or plus. The calibration procedure might need to be repeated re peated several s everal times to make the Offset Value smaller than 1 °C.
Calibration is complete once the Offset Value is less than 1°C.
Place
the
thermocouple
temperature probe in the front vent as shown in the right to measure the Room Temperature.
Secure
the
probe with a piece of tape.
Figure 10. Probe location to detect Room temperature
3.3 Block Calibration There are 3 sets of two Peltier modules( 40mm x 40mm) under the 96-well sample block. These 3 sets Peltier modules sit side by side. They are labeled as Left, Center and Right module set as shown in Figure 11. The Gradient temperature function is created by the temperature differences between these three Peltier module sets. To calibrate the sample block, a thermometer and a temperature probe, like Alpha Technics (http://www.alphatechnicsonline.com (http://www.alphatechnicsonline.com ), 4500 thermometer and SP230 0.2ml well probe, are needed. A multi-channel temperature thermometer system, such as Alpha Technics,
5020
thermometer
(16-channel),
or
Quanta
Biotech
(http://www.quantabiotech.com http://www.quantabiotech.com ), TAS Thermal Cycler Validation Systems or CYCLERtest (http://www.cyclertest.com/products.asp (http://www.cyclertest.com/products.asp ), DRIFTCON, is good for simultaneous temperature recording of different wells.
13
Right Peltier set Left Peltier set
Center Peltier set
Figure 11. 3 Peltier module sets location
Please follow the procedures below to calibrate the temperature of sample block. 1. Prepare a thermometer and place place the temperature well probe in 3 wells (D1, D6, D6, D12), as shown in Figure 12.
Well
probe:
Thermometer:
Alpha Technic,
Alpha
SP230
Technics,
4500
Figure 12. Temperature calibration setup
2. Enter System and select Lid Temperature. Temperature. Select 2 OFF in Lid Temperature Temperature function. Press “Accept”. Press “Exit”, and close the lid. 3. Select “Calibration Block” to display “Calibration Block” screen as shown shown in Figure 13. Please note “Uniform”(Uniformity) is a factory default setting. No adjustment is necessary!
14
MultiGene Service Manual
CAL I BRA TI ON>BL OCK
2007/ 1/ 5 11: 10 T me:
:
L ef t
+0. 4
( D1)
94. 0
Cent er
+0. 1
( D6)
94. 0
Ri ght
+0. 0
Uni f o f or m
+0. 0
( D12) 94. 0 Cal . Bl oc k ( A1, A12, H1, H12, D6)
BL K 4
Measured
( MAX- MI N) / 2- 0. 5
Calibration
Timer
Value (D1)
Value
BL K 94
F1
BL K 40
F2
+
-
F3
F4
HO T! Exi t
F5
Figure 13 “Calibration Block” screen
A.
Block temperature( Left, Center, Right ) calibration calibrat ion at 9
Press F1:BLK 94. The The temper temperatur aturee of the the sampl samplee block block will will go go up to 94 94
.
When the timer reaches five minutes (Time:05:00), record the Thermometer Readout of well D1 to calibrate the left Peltier module set (Left).
Using the formula below to calculate the Offset temperature value: Offset Value = Thermometer Readout(D1) – Measured Value (D1) Calculate the new calibration value by adding the factory calibration value and the offset value.
Input the sum of factory-calibrated Calibration Value(Left) and Offset Value into the Left Peltier module set’s Calibration Value column to calibrate the Left Peltier module set’s temperature. Press F3 or F4 to change the Calibration Value to minus or plus. It might be required to perform the procedures above several times to make the Offset Value less than +/-0.5 °C.
Temperature calibration of Left Sample Block is complete once the Offset Value is less than +/-0.5 +/ -0.5°C.
Repeat the same procedures above for Center(Well D6) and Right(Well D12) Peltier module set.
15
B.
Block temperatu temperature( re( Left Left,, Center Center,, Right ) calibratio calibration n at 40
Press F2:BLK 40. The temperature of sample block will go to 40
.
When the timer reaches five minutes (Time:05:00), record the Thermometer readout of well D1 to calibrate the left Peltier module set(Left).
Using the formula below to calculate the Offset temperature value: Offset Value = Thermometer Readout(D1) – Measured Value(D1)
Input the sum of factory-calibrated Calibration Value(Left) and Offset Value into the Left Peltier module set’s Calibration Value column to calibrate the Left Peltier module set’s temperature. Press F3 or F4 to change the Calibration Value to minus or plus. It might be required to perform the procedures above several times to make the Offset Value smaller than +/-0.5 °C.
Temperature calibration of Left Sample Block is complete once the Offset Value is less le ss than +/-0.5 °C.
Repeat the same procedures above for Center(Well D6) and Right(Well D12) Peltier module set.
16
MultiGene Service Manual
4 Software Upgrade MultiGene’s software can be upgraded by using a USB software programmer: Texas Instruments MSP-FET430 Flash Emulation Tool(FET) ( For more information, please visit www.ti.com/msp430 ) and a PC or notebook computer. The following items required for software upgrade include;
One Software Programmer: MSP-FET430UIF USB-to-JTAG USB-to-JTAG Emulation Interface
“FET-Pro430-Setup” Windows Installer Package file. [ If you can’t find it in
MSP430 CD-ROM, please download and unzip “FET-Pro430-Lite-Setup.zip” (P/N:CS014X01) file from http://www.elprotronic.com/download.html
to retrieve
“FET-Pro430-Setup” “FET-Pro430-Setup” file.]
One MSP-FET430UIF “READ ME FIRST” document
One USB cable, Type A/B
One 14-conductor cable
PC or notebook computer with Windows 2000 or XP
After the unit is turned on, the initial screen which contains software version will show up. Follow the instructions below to refresh the new software version to the thermal cycler. To ensure the performance of the product are within its specifications, the temperature of block, lid and room must be recalibrated after the software refresh.
4.1 Install the Software 1. Run “FET-Pro430-Setup” Windows Installer Package file. 2. Click “Next” button button on “FET-Pro430 “FET-Pro430 v1.10 v1.10 –Lite version” screen. 3. Choose “I Agree” and click “Next” button button on “License Agreement” screen. 4. Fill in “Name” and “Organization” and click “Next” button on “Customer Information” screen. 5. Choose “Everyone” or “Just me” and click “Next” button on “Select Installation Folder” screen. 6. Click “Next” button on “Confirm Installation” screen. 7. Click “Next” button button on “Very “Very Important: Read carefully.” carefully.” screen. 8. Click “Close” button on “Installation Complete” screen.
4.2 Install the Hardware 1. Plug in the MSP-FET430UIF software programmer to the USB Port of a personal computer 17
(PC), using provided cable extender (USB-A to USB-B). Connect 14-conductor cable with thermal cycler’s CN104 software upgrade connector and MSP-FET430UIF programmer as shown in Figure 14.
MSP-FET430UIF programmer 14-conductor cable: USB cable:
Pin 1 is Red.
Connect to a PC.
Figure 14. MSP-FET430UIF software programmer setup
2. The New New hardware has been found should be displayed, once the software programmer “
”
has been connected to your PC.
3. If your computer can’t recognize MSP-FET430UIF as a USB device for any reason, point the Hardware Installation Wizard to the appropriate folder where the corresponding driver information files are located on your hard disc. Drivers in the previously installed software are located(on a default installation) in directory: C:\Program Files\Elprotronic\FET-Pro430 Flash Programmer\USB-Driver\W Programmer\USB-Driver\WinXp inXp
4. Driver installation process will start. Note that Windows XP shows a warning that the driver is not certified by Microsoft. Ignore this warning and click “Continue Anyway”.
4.3 Reflash the New Software 1. Run “Lite FET FET-Pro430 Elprotronic” program. 2. Choose “Setup” in menu. Select “Connection/Deice Reset” option. Communication with the target devices can be selected in the “ Target’s Connection/Reset Options” dialogue screen..
3. Select “Standard JTAG” under “Communication with Target Device” column. Select “Any” under “Reset Options”. Select “Switch Vcc Off” under “Final Target Device Action”.
Select “TI’s FET,USB-FET or hardware compatible-uses TI’s MSP430.dll library” under “Used Adapter” column. Select
“USB USB” ” under “COM port” column. Click “OK”
button when all the selections are done. The screen will move into the software reflashing screen.
18
MultiGene Service Manual
4. In software reflashing screen, type:MSP430-F: G4619
under “Microcontroller
Type” column. 5. Click “ Open Code File” button to select the new software version’s version’s
path. The new
software file(Format: *.txt ) is provided by Labnet. Click “AUTO PROG.” button to reflash the new software. 6. Once the installation is complete, complete, the “Status” column will show “Pass”. 7. Turn On the unit to check the initial screen whether the software version has been upgraded.
19
5 Troubleshooting Please refer the following problem and action list to diagnose and resolve the problems. More troubleshooting flows are described in Section 5.2. Problem
Cause
Action
The display is off even when 1. Power is not reaching reaching the 1. Check power is switched on.
system. 2. Power
cord
is
not 2. Reconnect
properly. properly.
environment
power
3. Replace the power fuse.
4. Block fuse is blown out. 1. Operating
the
cord.
3. Power fuse is blown out. 4. Return °
source
voltage.
plugged into the socket
Can’t reach 4 C.
power
the
unit
for
service.
temperature 1. Operate the unit in a may
be
unsuitable.
environment temperature between 15 to 30 C. °
2. Electronic
cooling 2. Return
element may be damaged
the
unit
for
service.
or aged. Cycle time is too long
1. Operating temperature
environment 1. Operate the system in a may
be
unsuitable. 2. Electronic
between 15 to 30 C. °
cooling 2. Return
element may be damaged or aged. 3. Faulty temp. sensor. Lid heater does not work.
temperature environment the
unit
for
the
unit
for
service. 3. Return service.
1. Lid heater is set off.
1. Check Lid Temperature
2. Lid sensor problem
setting in System Mode under Calibration Lid. 2. Return
the
unit
for
service. No key entry sound.
1. Sound may currently be 1. Check Beeper setting in set off.
System Mode.
2. Faulty keys or keypad 2. Return wiring. The display goes off.
the
unit
for
service.
1. Faulty backlight.
1. Adjust the LCD contrast.
2. Faulty LCD panel
2. Return
the
unit
for 20
MultiGene Service Manual
service. Display is too dark or light
Backlight brightness is not Adjust adjusted properly. properly.
Lid will not close.
Display
Brightness
Potentiometer.
1. Foreign matter, between 1. Remove lid heater and sample
the
foreign
object or matter.
block. 2. Faulty
lid
lock 2. Replace lid.
mechanism. Error message appears.
Refer
to
list l ist
of
error Check the nature of the error
messages below. below.
and
take
the
suggested
actions.
5.1 Error Messages Message
Cause
Action
HEATER HEATER ERROR!
Lid temperature sensor problem.
Lid temperature exceeds 132 °C.
Lid temperature can’t reach the set
Reboot the unit.
temperature. BLOCK ERROR!
Block temperature sensor or heating Cool element problems.
The
unit
temperature,
can’t
down
the
room
temperature. Reboot the unit. reach
because
the
the
set room
temperature is too high.
The block temperature exceeds 108 °C.
ROOM ERROR!
Room temperature sensor problem. Reboot the unit
ROOM > 30 °C
The room temperature exceeds 30 Cool °C.
the
room
temperature. Reboot the unit.
Two running units are too close to Remove each other.
down any
objects
blocking the vents. Keep two running units at least 30cm from each e ach other.
21
5.2 Troubleshooting Flows Please refer the flows below to analyze the defect problems.
5.2.1 Beeper has no sound
Users turn off Beeper ?
Yes
Turn Turn ON Beepe Beeper r in System Mode
No Return to Labnet for Repair
5.2.2 LCM has no display There are a number items which may cause LCM display to fail. Please follow the flow chart below to identify the possible problems.
22
MultiGene Service Manual
LCM Backlight VR
LCM has no display
Check
No
LCM backlight NoVR?
Adjust
LCM
backlight VR
Yes AC power cable socket socket Check AC Power Cable
Fuse socket No
Insertion ?
Insert AC cable to socket tightly
Yes
Is the power fuse
Yes
Change
power
fuse
blown ?
LCM Panel cables
No Replace Are panel panel cables OK?
No
panel
front and
cables Yes Block Fuse Is Block Fuse burned ?
Yes
Digital Multimeter
Replace Sample Block
No Return to Labnet for repair. Block Fuse test : Open ( 23
∞
ohm ): Broken;
Short ( 0 ohm ) : OK
5.2.3 Buttons are not activated Front Panel Assembly ( P/N: TC96003-RP ) Buttons are not activated
Power On the machine
No
again?
Turn OFF and ON the machine
Yes
Keypad Cable
Replace the Front Panel Assembly?
No
Replace Front
LCM Cable
Back light Cable
the Panel
Assembly Yes Return to Labnet for Repair
24
MultiGene Service Manual
5.2.4 TE Module and PT-1000 Checkup a. Use a Digital Digital Multimeter (BM859CF ) to check PT1000’s PT1000’s Ohm values. values.
At 25 C,
Values = 1095 ~ 1097 ohm
At 25 C,
Values < or > 1095 ~ 1097 ohm
°
°
a
b
c
d
e
OK
f
NG
g
h
i
a, b, c, g, h, I : 6 sets TE module wire pairs d, e, f : 3 sets PT-1000 wire pairs
Programmable
Digital Multimeter : (BRYMEN BM859CF)
Meter
Impedance
( ABM3365 )
b. Use a Programmable Impedance Meter ( ABM3365 ABM3365 ) check TE’s TE’s Ohm values . Values = 2.10 ~ 1.60 ohm
At 25 C,
Values < or > 2.10 ~ 1.60 ohm
°
°
25
At 25 C,
OK
NG
6 Disassembly/Assembly Service engineers can follow the procedures below to disassemble the modules of the thermal cycler step-by-step. Service engineers can reverse the procedures below to assemble the modules. Please ensure the power cord has been unplugged during the assembly/disassembly procedures.
6.1 Front Panel Disassembly
1. Use clip to remove two grey rubber pads as 2. Release and remove the two screws as showed above.
showed.
3. To open the front panel, use one hand to
4. Pull out the three cables as showed, and
push the front of front panel and the other
then can disassemble the front panel.
hand to lift its rear.
26
MultiGene Service Manual
6.2 Sample Block Disassembly
Notch
1. Slide lid module to the middle position of 2. Use clip to remove the six rubber pads as its track and open it through two notches.
shown above.
3. Release and remove the two screws as 4. Release and remove the two screws as shown above.
shown above.
5. Release the four screws as shown above. 6. To remove the sample block module, hold Please don’t remove them.
the handle of the module and pull it out as shown direction.
27
6.3 Top Case Disassembly
1. Release and remove the nine screws as 2. Release and remove the three screws as shown above.
shown above.
3. Pull out the two cables as shown above.
4. This is the photo after pulling the two cables off.
5. Use left hand to hold the metal bracket as 6. When users open the top case, please note shown above. Pull it toward inside direction one black power switch cable maybe jammed when right hand lifts up the top case.
by a metal parts as shown.
28
MultiGene Service Manual
RS-232 cable
7. Please release the jammed cable. DON’T 8. Open the top case and pull out the RS232 use force to pull the cable before release it.
9. Top Top case disassembly disas sembly is complete.
29
cable as shown above.
6.4 Lid Module Disassembly
1. Release and remove the screw as show 2. Insert the two connectors of heater module above.
cable one by one to an square hole on case and pass though it to the outside of the case.
3. Pull these cables to the outside of top case 4. Use clip to take off the two rubber covers carefully.
as showed.
5. Push the lid module to the back end of its 6. Release and remove the inner two screws as track.
showed. You can’t do this step if the lid module isn’t on the back end position.
30
MultiGene Service Manual
7. Lift the side of lid module as showed.
31
8. The lid module disassembled.
6.5 Lid Knob Disassembly
1. Use clip to remove the two rubber pads as 2. Release and remove two screws as shown shown above.
above. Two screws can’t be seen if the lid module isn’t on the back end of its track.
3. Lift up the side of lid module as shown.
4. Turn the lid knob clockwise to lower down and separate the heater module.
5.
Turning
the
lid
knob
clockwise 6. Lid cover is disassembled.
continuously, the heater module will be separated as shown above. 32
MultiGene Service Manual
7. Flip the lid cover as shown above.
8. Release and remove the two screws as showed.
9. The lid knob is fully disassembled.
33
6.6 MCU Circuit Board Disassembly
1. Pull out the three cables as shown above.
2. Release and remove the four screws as shown above.
3.
MCU
circuit
board
disassembly
is
complete.
34
MultiGene Service Manual
6.7 Power Circuit Board Disassembly
1. The three cables are managed as shown 2. Move the cables away from the original above.
positon as shown above.
3. Release and remove the five screws as 4. Don’t remove the aluminum foil. shown above.
Fan cable
5. Open the power circuit board EMI 6. Pull out the system fan cable as shown shielding. The power circuit board can be above. seen. 35
7. Release and remove the two screws as 8. Release and remove the two screws as shown above.
showed.
9. Use both hands to hold the two sides of 10. Inner module is disassembled. metal bracket and lift up whole inner module.
11. Pull out the two power terminal connector 12. Release the eleven screws and pull out protection covers as shown above.
these cables as shown above.
36
MultiGene Service Manual
Copper Stand
13. Use 5 m/m hex head driver to release and 14. remove the copper stand as shown above.
37
Power
complete.
circuit
board
disassembly
is
6.8 System Fan Disassembly
1. Release and remove the four screws as 2. System fan disassembled. showed.
7 Spare
Parts
List
and
Exploded
Diagram Please follow the spare parts list below to order your required spare parts. For easy repairs, the spare parts are modularized. Only spare parts with check can be ordered. Please refer the Exploded Diagram to service the machine.
38
MultiGene Service Manual
7.1 Front Panel Assembly
PARTS LIST OF FRONT PANEL ASSEMBLY
Item
Part No.
Description
TC96003-RP Front panel assembly
Spare Parts
Yes
No
√
Including Item 1,2,3,4
49
207-000102-00 Front panel
√
N/A
50
210-000002-00 LCD sponge (L)
√
N/A
51
210-000001-00 LCD sponge (S)
√
N/A
52
215-000001-00 Membrane keypad
√
N/A
53
156-07p010-c1 LCD module WG240128B
√
N/A
54
150-p01c10-08 Wire DC 24V L200
√
N/A
55
206-000025-00 LCD bracket
√
N/A
(A)
202-130308-10 Screw PTS-4 3X8
√
N/A
(B)
201-530308-10 Screw SPW M3x8/w washer
√
N/A
39
7.2 Lid L id Assembly
40
MultiGene Service Manual PARTS LIST OF LID (SLIDE (SLID E COVER) COVE R) ASSEMBLY ASSEMBLY Item
Part No.
Description
TC96012-RP Lid(Slide cover) assembly
Spare Parts Yes No
Including Item
30,31,32,33, 34,35,36,(A), (C),(G),(L),(M)
√
30
207-000106-00 Top knob
√
N/A
31
206-000042-00 Knob pin
√
N/A
32
206-000043-00 Knob latch
√
N/A
33
205-074220-00 205-074220 -00 Spring 0.7x4.2x20 0.7x4.2x2 0
√
N/A
34
207-000103-00 Slide cover
√
N/A
35
206-000034-00 Bearing 6905 LLU/2A
√
N/A
36
206-000040-00 Bearing bracket
√
N/A
37
206-000020-00 Heater press screw
√
N/A
38
207-000112-00 Slide shaft (R)
√
N/A
39
207-000111-00 207-000111-00 Slide shaft (L)
√
N/A
40
205-454515-00 Spring Spring 0.45x4.5x15
√
N/A
41
206-000044-00 Knob pin fixer
√
N/A
42
206-000030-00 Latch link
√
N/A
43
206-000031-00 Latch cover
√
44
206-000021-00 Extend screw
√
N/A 44,45,46,47, 48,49,50,51, 52,(B),(F),(G) N/A
45
207-000110-00 Latch releaser
√
N/A
46
205-022912-00 205-022912 -00 Spring 0.2x2.9x12 0.2x2.9x1 2
√
N/A
47
206-000032-00 Heater base bracket
√
N/A
48
273-heater-00 Silicon rubber heater
√
N/A
49
206-000033-00 Heater bracket
√
N/A
50
205-684070-00 Spring 0.4x(6/8)x7
√
N/A
51
207-000107-00 Lid seal frame
√
N/A
52
210-020002-00 Seal PORON
√
N/A
(A)
202-130308-10 Screw PTS-4 3X8
√
N/A
(B)
201-530308-10 Screw SPW M3x8/w washer
√
N/A
(C)
201-404208-10 Washer PW-4.2x10x0.8 PW-4.2x10x0.8
√
N/A
(E)
202-130206-10 Screw PTS-4 2x6
√
N/A
(F)
206-000022-00 Heater bracket screw
√
N/A
(G)
201-120305-11 201-120305-11 Screw FMS M3X6 120D
√
N/A
(H)
201-140306-10 Screw BMS M3*6
√
N/A
√ T01-000081-00 Heater module assembly
41
(L)
202-140304-40 Screw BTS-4 3x4 Black
√
N/A
(M)
202-110204-40 Screw BITS 2x4 Black
√
N/A
7.3 Top Top Case Cas e Assembly
42
MultiGene Service Manual
PARTS LIST OF TOP CASE ASSEMBLY
Item
Part No.
Description
Spare Parts Including Yes No Item
19
207-000100-00 207-00010 0-00 Top case
√
N/A
20
207-000104-00 Slide guiding L
√
N/A
21
207-000105-00 Slide guiding R
√
N/A
22
207-000108-00 207-00010 8-00 Spring cap
√
N/A
23
205-056300-00 205-05630 0-00 Spring 0.6X5X30
√
N/A
14
206-000026-00 I/O bracket
√
N/A
25
193-pcr150-00 RS232_V03 PCB PCBA
√
N/A
26
122-42608r-00 Power SW JS-608RA-B1B-2
√
N/A
27
122-1 22-12 2101 101q-0 q-00 Powe Powerr sock socket et JR-1 JR-101 01-1 -1FS FS(Q (Q))
√
N/A
28
150-p01c10-01 Wire AC L=480mm
√
N/A
29
150-p01c10-05 Wire DC24V/0A5 L400
√
N/A
(A)
202-130308-10 Screw PTS-4 3X8
√
N/A
(B) (C) (D)
201-530308-10 Screw SPW M3x8/w washer 201-404208-10 Washer Washer PW-4.2X10X0.8 203-300507-10 Copper stand 4# 5X7
√
N/A N/A N/A
43
√ √
7.4 Bottom Case Assembly Assembly
44
MultiGene Service Manual PARTS LIST OF BOTTOM CASE ASSEMBLY
Item
Part No.
Description
Spare Parts
Yes
No
Including Item
1
207-000101-00 Bottom case
√
N/A
2
208-000011-00 Rubber foot RW3005
√
N/A
√
N/A
√
N/A
√
N/A
√
N/A
√
N/A
3 4 5 6 7
TC96004-RP Fan module assembly 206-000023-00 Main PCB bracket TC96006-RP MCU_V03 PCB PCBA 206-000028-00 Power supply bracket TC96007-RP Power supply SP500-24
8
206-000024-00 System bracket
√
N/A
9
206-000027-00 Heat-sink support bracket
N/A
10 11 12 13
TC96005-RP 201-300325-30 201-30032 5-30 206-000039-00 206-000039 -00 150-p01c10-06
√ √ √
14
√
N/A N/A N/A N/A
150-p01c10-07 Wire DC 24V/0A5 L300 p=12
√
N/A
15
150-p01c10-04 Wire DC 24V/0A5 L230 p=4
√
N/A
(A)
202-130308-10 Screw PTS-4 3X8
√
N/A
(B)
201-530308-10 Screw SPW M3x8/w washer
√
N/A
(C)
201-404208-10 Washer Washer PW-4.2X10X0.8
√
N/A
45
POWER_V05 PCB PCBA Stand off M3X25X6 Power PCBA shielding Wire DC 24V/0A5 L300 p=10
√
7.5 Fan Assembly
PARTS LIST OF FAN ASSEMBLY
Item
Part No.
Description
TC9600 TC96004-R 4-RP P Fan module module assemb assembly ly
Spare Parts Including Yes No Item 1,2,3,(C),(I),(K
√
)
16
123-12412 123-12412h-f1 h-f1 Fan PSD2412PMB PSD2412PMB1-(2) 1-(2)
√
N/A
17
208-000008-00 Fan bumper
√
N/A
18
206-000038-00 Fan protect frame
√
N/A
(C)
201-404208-10 Washer PW-4.2X10X0.8
√
N/A
(I)
201-130420-20 Screw PMS M4*20
√
N/A
(K)
201-200435-20 Nut M4x8 t=3.5
√
N/A
46
MultiGene Service Manual
7.6 Complete Assembly
47
PARTS LIST OF FINAL ASSEMBLY
Item
Part No.
Description
Spare Parts Including Yes No Item
60
275-pcrln2-00 Bottom case assembly
√
N/A
61
275-pcrln1-00 275-pcrl n1-00 Top Top case assembly
√
N/A
62
TC96012-RP Slide cover assembly
√
N/A
63
TC96003-RP Front panel assembly
√
N/A
64-1
TC96008-RP 96 wells block assembly
√
N/A
64-2
TC96009-RP 60 wells block assembly
√
N/A
65
208-000010-00 Screw rubber pad
√
N/A
(J)
201-110310-10 Screw TMS H M3x10
√
N/A
48
Labnet International Inc. PO Box 841, Woodbridge, NJ 08837, USA Tel: +732-417-0700 Fax: + +732-417-1750 732-417-1750 E-mail:
[email protected] Web Site: www.labnetlink.com
P/N: 402-PCR01-0708 Issue date: 2007/8
