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AS

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VE TO

ACID A M I D E S

15D·21>

CO

STERILE

Ergot IC[auiceps purpurea

for consciousness

in

that

derivatives into the

materials which

not this

to a.

jugs.

lysergic

fungus

cultivation

It

inform alien

We

treatment. of L8D·25. lysergic

with

of

acid-related

plett'

more difficultt

01t

wood.

ND

THE

(Clauiceps purpurea),

sp

LYSE GI

Lyse

ac -related

fu

foods),

ID

materials

ig ic Geotrichum candidum uc

bindweed

and

).

family

ud

occu naturall

of

sess

this

and

[which

in

the

ea vi

aw

is fo

dried

is

fo

ornamentals

Drugs

florists.

former to

21

Ti

pods

Dangerous

us

Ha (Argerei nervosa) (Riuea corymbosa) {Merremia tuberosaJ,oIoluique Convolvulus and Stietocardia.

ug

are

LA

To

ur kn wled

own

to

or ci

"n

ur OUT

only

procedures

the

may

by fo

in described remind if anyo

and place

materials

will

at em

confused wi

it

in

to

carry

FROM

before attempting

to is placed

milk

submerging the' days at 27°C. Large-Scale CuUivation followin 50 5.4

formula: Mannitol

10 mg Zinc 500

0g

of nutrient

sulfate (FeSOot

O)

L-tryptophan

ro

in

surface.

will

3) Extraction

impurities

in

by

35 discarding

using

fresh

ethyl

Adjust

the

ff excess

potassium volumes. IThis

IS'l

C ompa flY.)

be Add

of

crystallizes

dishes

evaporate.

240

in

ation extremely

dry.

be

PREPARATION

OF

ruaa

00

(MgS0 00

7H 0)

7Hi)1

0)

A-Z

C\.

to

Dissolve in distilled

acid

cotton-stoppered pH

in

slants li

by

27 C;

will

Large-Scal

Production in

ergot

th

give-n

'f,., ethanol by

.o

formula homogenize

to

Let growth

an

3) ·ExtradioD

to

in

ammonium

50/50

the

is

free

chloroform/isobutanol. dryness It

is

wild be germination.

'6 nther

support

.s

for

r-..lexican )..:"Inry

Ololiuqui

(Ril·l'Q

COT_vm

bosa

morning

Acid

to

.L

distilled

1g water.

it

are in

Yield

in

rind

if by

lm

it

fu ur

it stai

us

Mix

st

it

ex ac

in

to

evaporation.

Prepare

in this

in C'

th

LhE'

ruUow..

funm-l

If

.s

I"

3l'id

.h

rl -u ,.(, ;1

~as 11r.·,~un

10

,,,,,I

is

to to will

by water.

MAKING LSD-25

seeds. is

ka

ce sa

ug

LSD·25 FROM D·LYSERGlC ACID ~1:(}rl .u

in

xe VOII'

Icork

Diss

60.8

fluo

ce

to

lu

ab

in

hours.

mI in

wa er aye an

chloroform ex ac sodium

product accomplished

recrystallization.

LSD-25

I.

nask combine two volumes of in

hOI

agra

el

RFlS*.IIl1lI.L~ P14.!NW

N~

o" Vt ml

~!NE

by

2° ut yd az

fOT

se eral

urs. Iso-lyser

an ce at reaction flask so-lyser

chill

aci

ac

fr will crystallize. in

ll

28.2

in

yd azi

fo

0. 0.

No

acid is stirring. Afte.r saturated sodium

Tr

to ke

5-10 ar

um

with ut

ub

in

so ut well an ers. ss ed

measure et er

Allow this to stand in total Iy

LSD-25.

so

se

24

ci

am

us

co

ed to

DIETHYLAMIDE TO LSD·25

un et an St in li tassium ydroxid soluti fo ci ke ut sl

um

Let

No mal

etha-

xtur ut

as

hydroxide separatory

equal um ch fo et ylen dichloride}, sh ke el set e, co ect ch fo ayer an sca at ayer epea ext act wi an er POrtiOD ch fo Wash ch fo um 25 in ater Evap at co ed ch fo ex ac un er euc ur ac et am an so a- yser ac et am e. Th so-LSD sych ac e. ut uc ay safe used 13

light

in benzene/chloroform

.l isomers.

As the

too can

in

li ht-proof container. Collect

wash it

in

then rechromatographe

Th collecte under allowed to rated

than

alumina

ct

as described

fastest

reduced

be

that

In

lu

uci.

c.c. CuSO".5HzO

SnCI2·2R20,

4H

1g C.c.

Oa WO".

S2

100 rn

8r80

ST

come in ly sterilized

in

15

Ga Cover hair the

mask

Ie

the

clean

enter no animals, in the room sterilized. the medium and inoculum. Do not lean over the work. Avoid swi."t create drafts. Be neat and. keep all materials ithi reach. '5

cap

shower

SUPPLIERS

wood

untreated

wood

seeds,

Ca

materials

P.

chem ca Rather than attempt the

ll of these

book are available from States. we refer

tains it may be

If there is an diff cult under

C/.av.icepspurpurea in let the collection.

cultiva-

in

compare

black color.

Books

vailable PR

ER

y e

Th

Fundamen

In Techniques,

$1.50

PL

as Available at

OI

lTl- ll

Wh lesal inqui ie to Merchan isin 41 N. 3r adel a,

Ser ic 23

me ic