LABORATORIO-BIOQUI-N01

“AÑO DE LA DIVERSIFICACIÓN DIVERSIFICACIÓN PRODUCTIVA PRODUCTIVA Y DEL FORTALECIMIENTO DE LA EDUCACIÓN” E.A.P. INGENIERÍA AGROINDUSTRIAL

DOCENTE:

CARMEN YZÁSIGA BARRERA

CURSO:

BIOQUÍMICA

INTEGRANTES: • • •

BLAS ESPINOZA NEYSSER CARHUAYANO SANTAMARIA ELIAS NUÑEZ MORALES SELENI CHIMBOTE – PERÚ 2015

1

Universi! N!"i#n!$ e$ S!n%!

TÍTULO

“FOTOCOLORIMETR&A”

INTRODUCCIÓN P!r! '#er es!rr#$$!r e (!ner! e)"!* e$ %e(! e+e(#s %ener en ",en%! -,e $! "#$#ri(e%r.! es ,n! e $!s %/"ni"!s (0s ,s!!s en $! +i#-,.(i"! ",!n# se re-,iere e%er(in!r $! "#n"en%r!"in e ,n "#(',es%# # 2r,'# e "#(',es%#s -,e 3#r(!n '!r%e e ,n! s#$,"in "#$#re!!4 5! se! n!%,r!$(en%e # 6e"6! !s. (ei!n%e re!""i#nes -,.(i"!s es'e".)"!s7 Se !n!$i*! $! "#n"en%r!"in e ,n! (,es%r! '#r e$ "#$#r -,e es%! 'resen%!4 3,n!(en%0n#se en $! (!5#r # (en#r !+s#r"in e $,* e !",er# !$ n8(er# e (#$/",$!s is,e$%!s ",5! %#n!$i! e'ener0 e $! "!'!"i! e !+s#r+er ,n! , #%r! $#n2i%, e #n! e $,*7 [WALTON Y REYES, 1998. Análisis quíi!"#. L#s ",er'#s "#$#re!#s !+s#r+en $,* e e%er(in!! $#n2i%, e #n! 5 re9e!n e$ res%# e$ es'e"%r# $,(in#s# visi+$e e invisi+$e7 C!! (#$/",$! !+s#r+e $,* e !",er# ! $! "#n"en%r!"in en -,e se en",en%re en%r# e $! s#$,"in 5 ! "!! $#n2i%, e #n! e $,* "#rres'#ne ! ,n is%in%# nive$ e ener2.! '!r! e;"i%!r s,s e$e"%r#nes7 Si "#nsier!(#s -,e "!! (#$/",$! !+s#r+e $,* e !",er# ! $! "#n"en%r!"in en -,e se en",en%re en%r# e $! s#$,"in4 ! (!5#r !+s#r"in e $,* es (en#r $! $,* %r!ns(i%i!7 Es%#s 3!"%#res es%0n "#nsier!#s en $! $e5 e L!(+er% 5
=


O$%ETI&OS • •

•

O+%ener e$ es'e"%r# e !+s#r"in e ,n! s,s%!n"i!7 Pre'!r!r ,n! ",rv! e "!$i+r!"in '!r! ,n! s,s%!n"i! "#$#re!!7 De%er(in!r $! "#n"en%r!"in e ,n! (,es%r! 'r#+$e(! (ei!n%e 3!"%#r e "!$i+r!"in 5 ",rv! e "!$i+r!"in7

'ATERIALES Y '(TODOS •

Se e'#si% s,$3!%# e "#+re >C,SO?@ en ,n %,+# e ens!5#4 se2,i# e ($ e !2,! es%i$!! 5 se n#(+r $!s (,es%r!s '#r e$ #ren res'e"%iv# '!r! $,e2# ser $$ev!# !$ es'e"%r# 3#%(e%r# 5 '#er (eir s, !+s#r+!n"i!7 L!s "!n%i!es ,%i$i*!!s en $!s  i3eren%es (,es%r!s es%0n en e$ 2r0)"#:

A
•

Se e'#si% 0,25 ml e i"r#(!%# e '#%!si# > H =Cr=O@ en ,n %,+# e ens!5#4 se2,i# e 0,75 ml e !2,! es%i$!!4 5 se n#(+r "#(# $!

B

Universi! N!"i#n!$ e$ S!n%! (,es%r! n1 '!r! $,e2# ser $$ev!# !$ es'e"%r# 3#%(e%r# 5 '#er (eir s, !+s#r+!n"i!7

•

Se e'#si% 0,50 ml e i"r#(!%# e '#%!si# > H =Cr=O@ en ,n %,+# e ens!5#4 se2,i# e 9,50 ml e !2,! es%i$!!4 5 se n#(+r "#(# $! (,es%r! n= '!r! $,e2# ser $$ev!# !$ es'e"%r# 3#%(e%r# 5 '#er (eir s, !+s#r+!n"i!7

•

•

Se e'#si% 0,70 ml e i"r#(!%# e '#%!si# > H =Cr=O@ en ,n %,+#

e ens!5#4 se2,i# e 9,30 ml e !2,! es%i$!!4 5 se n#(+r "#(# $! (,es%r! nB '!r! $,e2# ser $$ev!# !$ es'e"%r# 3#%(e%r# 5 '#er (eir s, !+s#r+!n"i!7

•

Se e'#si% 1,0 ml e i"r#(!%# e '#%!si# >H =Cr=O@ en ,n %,+# e ens!5#4 se2,i# e 9,0 ml e !2,! es%i$!!4 5 se n#(+r "#(# $! (,es%r! n? '!r! $,e2# ser $$ev!# !$ es'e"%r# 3#%(e%r# 5 '#er (eir s, !+s#r+!n"i!7

?


•

Se e'#si% 2,0 ml e i"r#(!%# e '#%!si# >H =Cr=O@ en ,n %,+# e ens!5#4 se2,i# e 8,0 ml e !2,! es%i$!!4 5 se n#(+r "#(# $! (,es%r! n '!r! $,e2# ser $$ev!# !$ es'e"%r# 3#%(e%r# 5 '#er (eir s, !+s#r+!n"i!7

•

Se e'#si% ?,0 ml e i"r#(!%# e '#%!si# >H =Cr=O@ en ,n %,+# e ens!5#4 se2,i# e ,0 ml e !2,! es%i$!!4 5 se n#(+r "#(# $! (,es%r! n '!r! $,e2# ser $$ev!# !$ es'e"%r# 3#%(e%r# 5 '#er (eir s, !+s#r+!n"i!7

•

Se e'#si% 4= ($ e 'er(!n2!n!%# e '#%!si# >HMnO?@ en ,n %,+# e ens!5#4 se2,i# e 4 ($ e !2,! es%i$!! 5 se n#(+r "#(# $!



Universi! N!"i#n!$ e$ S!n%! (,es%r! n1 '!r! $,e2# ser $$ev!# !$ es'e"%r# 3#%(e%r# 5 '#er (eir s, !+s#r+!n"i!7

•

Se e'#si% 4= ($ e 'er(!n2!n!%# e '#%!si# >HMnO?@ en ,n %,+# e ens!5#4 se2,i# e 4 ($ e !2,! es%i$!! 5 se n#(+r "#(# $! (,es%r! n1 '!r! $,e2# ser $$ev!# !$ es'e"%r# 3#%(e%r# 5 '#er (eir s, !+s#r+!n"i!7

•

Se e'#si% 4= ($ e 'er(!n2!n!%# e '#%!si# >HMnO?@ en ,n %,+# e ens!5#4 se2,i# e 4 ($ e !2,! es%i$!! 5 se n#(+r "#(# $! (,es%r! n1 '!r! $,e2# ser $$ev!# !$ es'e"%r# 3#%(e%r# 5 '#er (eir s, !+s#r+!n"i!7

•

Se e'#si% 4= ($ e 'er(!n2!n!%# e '#%!si# >HMnO?@ en ,n %,+# e ens!5#4 se2,i# e 4 ($ e !2,! es%i$!! 5 se n#(+r "#(# $!



Universi! N!"i#n!$ e$ S!n%! (,es%r! n? '!r! $,e2# ser $$ev!# !$ es'e"%r# 3#%(e%r# 5 '#er (eir s, !+s#r+!n"i!7

•

Se e'#si% 4= ($ e 'er(!n2!n!%# e '#%!si# >HMnO?@ en ,n %,+# e ens!5#4 se2,i# e 4 ($ e !2,! es%i$!! 5 se n#(+r "#(# $! (,es%r! n '!r! $,e2# ser $$ev!# !$ es'e"%r# 3#%(e%r# 5 '#er (eir s, !+s#r+!n"i!7

•

Se e'#si% 4= ($ e 'er(!n2!n!%# e '#%!si# >HMnO?@ en ,n %,+# e ens!5#4 se2,i# e 4 ($ e !2,! es%i$!! 5 se n#(+r "#(# $! (,es%r! n '!r! $,e2# ser $$ev!# !$ es'e"%r# 3#%(e%r# 5 '#er (eir s, !+s#r+!n"i!7

RESULTADOS )

O$TENCIÓN DEL ES*ECTRO DE A$SORCIÓN



Universi! N!"i#n!$ e$ S!n%! Se !n!$i* $! !+s#r+!n"i! en "#(',es%#s "#(# e$ C,SO ?4 J =Cr=O 5 HMnO ? "#n seis (,es%r!s e "!! ,n# en i3eren%es "#n"en%r!"i#nes4 ! ,n! e%er(in!! $#n2i%, e #n! >n(@7

1. SUL+ATO DE CO$RE CuSO- Le"%,r! en e$ es'e"%r#3#%(e%r# !  n( e $#n2i%, e #n!7

SULFATO DE

/1

Y 1

4= 4 4 14 =4 ?4

4B 41 41 4= 4? 41=

COBRE 10 %

I II III IV V VI

K1: CONCENTRACIÓN Y1: A
0. DICRO'ATO DE *OTASIO 0C20O3 Le"%,r! en e$ es'e"%r#3#%(e%r# !  n( e $#n2i%, e #n!7

DICROMA TO DE

/1

Y 1

4= 4 4 14 =4 ?4

4 4? 4 411 4?B 4

POTASIO 0,1 %

I II III IV V VI

4. *ER'AN5ANATO DE *OTASIO 6'nO-



Universi! N!"i#n!$ e$ S!n%! Le"%,r! en e$ es'e"%r#3#%(e%r# ! ? n( e $#n2i%, e #n!7

PERMANGA NATO DE

/1

Y 1

POTASIO

I II III IV V VI

4= 4 1 4 41  4 4= 1 14 4  =4 4 ? ?4 41 

)

C7LCULO DE LA CONCENTRACIÓN DE UNA SOLUCIÓN

C#n $#s !%#s #+%eni#s !n%eri#r(en%e e $! !+s#r+!n"i! e$ C,SO ?4 J =Cr=O 5 HMnO? se "#ns%r,5e ! ",rv! e "!$i+r!"in 5 e$ 3!"%#r e "!$i+r!"in '!r! "!! ,n# e e$$#s 1. CUR&A DE CALI$RACIÓN DEL SUL+ATO DE CO$RE CuSO-



Universi! N!"i#n!$ e$ S!n%! 7 7 7 7? 7B 7= 71  

=

?

SULFATO DE



/01



1

1=

1?

1

1

/1 Y 1

COBRE 10 %

7=  7= II 7? III 17 IV V ?7 VI 17

7  7 7 7= 71? 7

I

K=1: CONCENTRACIÓN = K1 Y1: CONCENTRACIÓNA
FACTOR DE CALI
I@

F . C =

0.003 0.421

=¿

; 1B F . C =

41=B

II@

FC   ESTNDAR Q

F . C =

0.018 0.421

=¿

4?=B???= 0.010

=¿

F . C =

0.025

=¿

1


0. CUR&A DE CALI$RACIÓN DEL DICRO'ATO DE *OTASIO 0C20O3 7 7 7 7? 7B 7= 71 



=

?





/01

/1 Y 1

II III

7=  7= 7?

IV V VI

17 ?7 17

71 = 7= 7?  711 7 7B?

DICROMA TO DE POTASIO

1

1=

1?

1

1

0,1 %

I

FACTOR DE CALI
F . C =

0.005 3.08

=¿

14=BB ; 1 

41= F . C =

B

II@


F . C =

0.004 3.08

=¿

14=1= ; 1B 

41B 0.0007

=¿

F . C =

0.0011

=¿

11


4. CUR&A DE CALI$RACIÓN *OTASIO 6'nO-

DEL

*ER'AN5ANATO

7 7 7 7? 7B 7= 71 

PERMA

/0

NGANA TO DE POTASI

1



=

?

/1 Y 1

O

I

II

III

7  = 7 = 7 ?

17  V ?7  VI 1 7 IV

7 1  7   7 1?  7  71  7 





1

1=

1?

1

1

DE

1=


FACTOR DE CALI
I@

F . C =

0.071 0.319

=¿

II@


F . C =

0.019 0.319

4===B= F . C =

0.021

IV@

=¿

F . C =

=¿

0.056 0.319

711=B

=¿

41?B

DISCUSIÓN Se28n

J!r'er >=@4

'!r!

s!"!r

$#s

i3eren%es

(e%##s

-,e

,%i$i*!ri!(#s en e$ in3#r(e s#n e$ !n!$i%i"# # e$ (e%## 2r!)"#7 C#('r#+!n# es%# en $! 'r0"%i"! e $!+#r!%#ri# ne se e(#s%r -,e 2r!"i!s !$ es'e"%r#s"#'i# '#e(#s (eir $! !+s#r+!n"i! e (,"6#s "#(',es%#s7 A$ "#n#"er e$ !e",!# ,s# e$ es'e"%r#3#%(e%r# 'er(i%i #+%ener res,$%!#s "#n !$%! "!$i! !n!$.%i"! en $!s (ei"i#nes -,e s#n e(i%i!s '#r /s%e7 En e$ e;'eri(en%# e $e"%,r! e$ es'e"%r# e !+s#r"in '!r! s#$,"i#nes "#$#re!!s4 se #+%,vier#n !%#s -,e "#rres'#nier#n e+i!(en%e !

1B


"!! r!n2# e $#n2i%, e #n! '%i(! e "!! "#$#r ini"!# '#r $! %e#r.!7 Es%#s 3,er#n re3eren%es !$ Di"r#(!%# e '#%!si#7 P!r! $! s#$,"in e$ Di"r#(!%# e '#%!si# en $! $#n2i%, e #n! -,e se e%er(in (!5#r !+s#r+!n"i! en e$ se;%# %,+# >7@ en n(7 Se28n $e6nin2er4

$! "#n"en%r!"in e ,n! s,s%!n"i! %iene re$!"in

ire"%!(en%e 'r#'#r"i#n!$ "#n $! !+s#r+!n"i!7 En $! 'r0"%i"! se %r!+! %enien#

en

",en%!

e$

"#n"e'%#

e

3#%#"#$#ri(e%ri!

5

,n!

3,n!(en%!! res',es%! ! $#s 'r#"ei(ien%#s -,e re!$i*!(#s7 D#ne $#s res,$%!#s n# s!$ier#n e !",er# "#n $# es'er!# '#r -,e en e$ s,$3!%# e "#+re e$ %,+# 1 s!$i# "#n (,"6! (!s !+s#rv!n"i! -,e en e$ %,+# =4B 5 ? $# ",!$ e+eri! ser $# "#n%r!ri# ',es i"6# %,+# %eni! $! (en#r "#n"en%r!"i#n e s,$3!%# e "#+re ',es se28n $# es%,i!# $! "#n"enn%r!"i#n es ire"%!(en%e 'r#'#r"i#n!$ "#n s, !+s#r+!n"i! $# ",!$ ! (en#r "#n"en%r!"i#n seri! (en#r !+s#r+!n"i!7 De+i# ! $! ine;!"%i%, e $#s res,$%!#s se e(#s%r -,e $#s (.ni(#s ",!r!#s s#n ,%i$i*!#s '!r! ,n! (e#r 're"isin 5 e;!"%i%,7

CONCLUSIONES Se "#n#"i e$ !e",!# ,s# e$ es'e"%r(e%r# 'er(i%ien# #+%ener res,$%!#s e $! !+s#r+!n"i! e "!! "#(',es%# >s,$3!%# e

"#+re

>C,SO?@4

i"r#(!%#

e

'#%!si#

>J=Cr=O@

5

e$

'er(!n2!n!%# e '#%!si# >HMnO?@@ en i3eren%es "#n"en%r!"i#nes4 '!r! ,n '#s%eri#r !n0$isis en $! "#ns%r,""in e 2r0)"!s -,e 'resen%!+!n $! ",rv! res'e"%# ! "#n"en%r!"in 5 !+s#r+!n"i!7

1?


Se e%er(in $! $#n2i%, e #n! '%i(! ! $!s %res s#$,"i#nes "#$#re!!s %enien# '!r! e$ s,$3!%# e "#+re >C,SO?@  n(4 '!r! e$ i"r#(!%# e '#%!si# >J=Cr=O@4  n( 5 '!r! e$ 'er(!n2!n!%# e '#%!si# >HMnO?@4 ? n(7 Se "#ns%r,5 $! ",rv! e "!$i+r!"in '!r! $!s s,s%!n"i!s "#$#re!!s (ei!n%e e$ (/%## e $#s (.ni(#s ",!r!#s 5 !s. !r ,n (!5#r !,s%e ! $! $ine!$i! e $! ",rv! 5! #+%eni! "#n $#s res,$%!#s e$ 3#%(e%r#7 Se e('$e e$ 3!"%#r e "!$i+r!"in "#(# #%r# (/%## '!r! e%er(in!r $! "#n"en%r!"in e ,n! s#$,"in4 iviien# $! "#n"en%r!"in e $! s#$,"in es%0n!r en%re $! !+s#r+!n"i!7

RECO'ENDACIONES

•

•

•

U%i$i*!r $#s (!%eri!$es e $!+#r!%#ri# "#n s,(# ",i!# 5 se2,ir !$ 'ie e $! $e%r! $!s re"#(en!"i#nes -,e n#s ! $! 'r#3es#r! e $!+#r!%#ri#7 D!r $! 'r#'#r"in ini"!! e "!! "#(',es%# "#(# s#n e$ s,$3!%# e "#+re4 'er(!n2!n!%# e s#i#4 i"r#(!%# e '#%!si# 5 !2,! es%i$!! '!r! %r!%!r e #+%ener $#s !%#s "#rre"%#s en e$ es'e"%r#3#%(e%r#7 Li('i!r +ien $#s ,%ensi$i#s e$ es'e"%r#3#%(e%r# '!r! "#$#"!r $#s "#(',es%#s 5 %r!%!r e -,e n# 6!i2! (,"6# (!r2en e err#r7

1


•

De!r "#(# es%,v# e$ es'e"%r#3#%(e%r# '!r! -,e $,e2# $# ',e!n ,%i$i*!r "#n %#! n#r(!$i!

CUESTIONARIO 1. CU7L CREES UE ES EL '(TODO '7S E/ACTO *ARA O$TENER LA CONCENTRACIÓN DE UNA 'UESTRA *RO$LE'A, EL 5R7+ICO O EL ANALÍTICO: *OR U(: E$ (/%## !n!$.%i"# '#r-,e e$ es'e"%r#3#%(e%r# es ,n ins%r,(en%# es'e"i!$i*!# '!r! e$ !n0$isis -,.(i"# -,e (ie en 3,n"in e $! $#n2i%, e #n!4 $! re$!"in -,e e;is%e en%re $#s v!$#res e ,n! (is(! (!2ni%, 3#%#(/%ri"! re$!%iv# ! r!i!"i#nes 5 $!s "#n"en%r!"i#nes # re!""in -,.(i"!s e ,n! (,es%r!7 0. U( +ACTORES ALTERAN LA LEY DE LA'$ER Y $EER: De+e(#s

%ener en ",en%! -,e $! Le5 e L!(+er 5
3!"%#res -,e $! !$%er!n4 en%re es%!s $i(i%!"i#nes es%0n: 

LIMITACIONES PROPIAS: L! $e5 e L!(+er  is#$,"i#nes i$,i!s ! "#n"en%r!"i#nes e$ #ren e 1= M@4 ! '!r%ir e ,n! "#n"en%r!"in 41M e('e*!r0 ! 6!+er esvi!"i#nes e $! $ine!$i!7 Se i"e en%#n"es -,e '#r en"i(! e es%e v!$#r $! re"%! se ",rv! e+i# ! -,e ! (ei! -,e !,(en%! $! "#n"en%r!"in e es'e"ies !+s#r+en%es4 es%!s se v!n !'r#;i(!n#

1

Universi! N!"i#n!$ e$ S!n%! en%re e$$!s 6!s%! -,e se '#ne en (!r"6! $!s in%er!""i#nes e$e"%r#s%0%i"!s4 !$%er!n# $! "!'!"i! e $!s es'e"ies ! !+s#r+er ! ,n!s e%er(in!!s $#n2i%,es e #n!7 

LIMITACIONES DE


DESVIACIONES INSTRUMENTALES: A ve"es en e$ $!+#r!%#ri# n# se #+%iene ,n! +,en! ",rv! e "!$i+r!# '#r-,e ! ve"es $#s (#n#"r#(!#res e $#s es'e"%r#s se es!,s%!n 5 '#r $# %!n%# %ene(#s es'$!*!# e$ (0;i(# e $#n2i%, e #n!7 En e$ (0;i(# $#s "#e)"ien%es e !+s#r%ivi! (#$!r v!n ! ser (0s '!re"i#s7 Si n# es%!(#s en e$ (0;i(#4 $#s "#e)"ien%es e !+s#r%ivi! (#$!r v!r.!n (0s +r,s"!(en%e 5 n# #+%ene(#s ,n %r!(# re"%# en $! ",rv! e "!$i+r!#7

4. EL COLOR UE E/I$E UNA SUSTANCIA EST7 DETER'INADA *OR LA LU; UE TRANS'ITE O UE A$SOR$E:

Es%0 e%er(in!! '#r $! $,* -,e !+s#r+e4 ! ener2.! %#%!$ e(i%i! en 3#r(! e $,* es sie('re (en#r ! $! ener2.! %#%!$ !+s#r+i! 5 $! i3eren"i! en%re !(+!s es isi'!! en 3#r(! e "!$#r7 En $! (!5#r.! e $#s "!s#s $! $#n2i%, e #n! e(i%i! es (!5#r 5 '#r $# %!n%# e (en#r ener2.! -,e $! !+s#r+i!4 sin e(+!r2#4 si $! r!i!"in e e;"i%!"in es in%ens!4 es '#si+$e '!r! ,n e$e"%rn !+s#r+er #s 3#%#nes en es%! !+s#r"in +i3#%ni"!4 $! $#n2i%, e #n! e(i%i! es (0s "#r%! -,e $! !+s#r+i!4 sin e(+!r2# en !(+#s "!s#s $! ener2.! %#%!$ e(i%i! es (en#r -,e $! ener2.! %#%!$ !+s#r+i!7

1


En 2ener!$ $!s s,s%!n"i!s 9,#res"en%es !+s#r+en ener2.! en 3#r(! e r!i!"in e$e"%r#(!2n/%i"! e #n! "#r%! >' e r!i!"in 2!((!4 r!5#s ;4 UV4 $,* !*,$4 e%"@4 5 $,e2# $! e(i%en n,ev!(en%e ! ,n! $#n2i%, e #n! (0s $!r2!4 '#r ee('$# en%r# e$ es'e"%r# visi+$e $#s ee('$#s (0s n#%!+$es e 9,#res"en"i! #",rren ",!n# $! $,* !+s#r+i! se en",en%r! en%r# e$ r!n2# ,$%r!vi#$e%! e$ es'e"%r# invisi+$e !$ ## 6,(!n# 5 $! $,* e(i%i! se en",en%r! en $! re2in visi+$e7

RE+ERENCIA $I$LIO5R7+ICA 

A$+er%4 R 5 F7 D!nie$! >1@4 Fisi"#-,.(i"!7 =!7 Rei('resin7 C#n%inen%!$ S7A7 e C7V7 M/;i"#4 1



A5res4 G7 >1@4 An0$isis ,.(i"# C,!n%i%!%iv#7 =!7 Ei"in7 J!r$!4 M/;i"#7



J!r'er :



J#rn!4 E7 >1@4 L! "/$,$!7 M,$# I e


Le6nin2er:

LIN6O5RA+IA

= EDICIÓN
PRINCIPIOS DE
1


http://www.espectrometria.com/espectrometra_e_a!sor ci"  http://es.wi#ipeia.or$/wi#i/Espectro%ot&'(&)(metro 

LABORATORIO-BIOQUI-N01

Recommend Documents