Laboraory Facilities and Equipment in a Biotech Laboratory

3. List of available equipments in the biotech unit University of Southern Mindanao Agricultural Research Center (USMARC). Laboratory Facilities

Uses

1.

Thermal cycler

2.

Microcentrifuge

3.

Distilling apparatus

4.

Fumehood

5.

Pipettor/ Micropipettor

Used to separate liquid mixtures into fractions of different compositions by means of distillation. a ventilated enclosure in which harmful volatile chemicals can be used or kept. Used to suck small amounts of liquid for transfer or measurement.

6.

Vortex

Used to mix small vials of liquid.

7.

Water bath

8.

Horizontal gel electrophoresis

Used to maintain water at a constant temperature for microbiological incubations. Used to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge. Used for the imaging and documentation of nucleic acid and protein suspended within polyacrylamide or agarose gels. Used as a thickener and suspending agent.

apparatus 9.

Gel documentation system

10.

Polyacrylamide gel

used to amplify segments of DNA via the polymerase chain reaction (PCR) used to separate materials from small biological samples

electrophoresis 11.

Microwave oven

Used to heat foods by passing microwave radiation through it.

12.

Stirrer/ hot plate

Used to heat glassware or its contents.

13.

Electronic digital balance/

Used to measure small mass in the sub-milligram range.

 Analytical balance 14.

Biofreezer

Used for storage of vaccines, reagents, cultures, medicines and blood samples. Used for making ice.

15.

Ice maker

16.

Autoclave/ sterilizer

17.

Binder

18.

Dual action shaker

Used to sterilize equipment and supplies by subjecting them to high pressure saturated steam at 121 °C (249°F) for around 15 –20 minutes depending on the size of the load and the contents. Used to reduce the absorption of phosphate and taken with meals and snacks. Used for shaking of lab glassware.

19.

Beakers

Used for stirring, mixing and heating liquids

20.

Cylinders/ graduated cylinders

Used to measure the volume of a liquid.

21.

Erlenmeyer flask

22.

Syringe

23.

Thermometers

Used to mix by swirling, without risk of spillage, making them suitable for titrations. Used to take in and expel a liquid or gas through an orifice at the open end of the tube. Used to measure temperature or atemperature gradient.

Biotech 111 WORKSHEET Laboratory Exercise 2 Familiarization of Laboraory Facilities and Equipment in a Biotech Laboratory NAME: Glennjade S. Llenado_____________________ YR/CR/SEC: _4-BSInfoSys__ LAB INSTRUCTOR: ___________________________ LAB SCHED: _____________ 1. Laboratories housed at the Biotechnology Unit, University of Southern Mindanao Agricultural Research Center (USMARC). Laboratory 1. Plant Tissue Culture Laboratory

2. Isozyme Laboratory 3. Virus  – Indexing laboratory 4. Animal Science Laboratory 5. Molecular and biotechnology lab

Uses and Importance Focuses on the reproduction of exact copies of plants that produce particularly good flowers, fruits, or have other desirable traits. To quickly produce mature plants. The production of multiples of plants in the absence of seeds or necessary pollinators to produce seeds. The regeneration of whole plants from plant cells that have been genetically modified. Focuses on the electrophoretic analyses of genetically conditioned enzyme variation by separating multiple forms of enzymes in a solid matrix. Focuses on checking the transmission of virus through tissue- cultured plants by detecting and destroying the virus. It aims at supplying virusfree plant material to the farmer community. Focuses on the humane care and treatment of laboratory animals, as well as the quality research that leads to scientific gains that benefit people and animals. Focuses on study and modifications nucleic acids and proteins for applications in areas such as human and animal health, agriculture, and the environment.

2. Laboratory facilities that might be added to the laboratory at Biotech unit University of Southern Mindanao Agricultural Research Center (USMARC). 1.

Laboratory Facilities Ganong's Potometer / Photosynthesis Apparatus

2.

clinostat

device which uses rotation to negate the effects of gravitational pull on plant growth (gravitropism) and development (gravimorphism).

3.

Digital Dry Baths

Used to provide a controlled dry heat environment for test tubes and other containers.

4.

Bactizapper/ microsterilizer Viscometer Refractometer Hygrometer

5. 6. 7. 8.

The LA-H brushing machine

9. Platform balance 10. Biomixer and minimixer

Uses used for measuring the rate of water uptake of a leafy shoot.

Uses infrared heating to sterilize micro organisms. Used to measure the viscosity of a fluid. Used to get the measurement of an index of refraction Used for measuring the moisture content in the atmosphere. Used for batch and continuous flow polishing, hulling or scarifying of small volumes of seed or grain samples prior to cleaning and grading Used for determining weight of small objects/samples. Uses tri-directional motion for mixing in blood & centrifuge tubes