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Chapter 3: Nucleotides, Nucleic Acids, and Genetic Information Matching A) B) C) D) E) F) G) H) I) ) ") $) %)
diploid phosphodiester probe pyrimidine introns cloning transformed exons ampicillin a!toradiography chain#terminator p!rine ester
1& Cytosine' !racil' and thymine are deri(ati(es of & Ans* D +ection ,&1 Diffic!lty* Easy $earning -b.ecti(e* Nucleotides /& 0!cleic acids are composed of n!cleotides that are are lined by linages& Ans* B +ection ,&/&A Diffic!lty* Easy $earning -b.ecti(e* Introduction to Nucleic Acid Structure ,& -rganisms composed of cells 2ith t2o sets of chromosomes are called organisms& Ans* A +ection ,&,&A Diffic!lty* Easy $earning -b.ecti(e* Overview of Nucleic Acid unction 3& E!aryotic genes often contain regions that are transcribed transcribed and later excised from the 40A' 40A' called & Ans* E +ection ,&5&D Diffic!lty* %oderate $earning -b.ecti(e* Overview of Nucleic Acid unction
1 This is sample only, Download all chapters at: testbankreal.com
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5& Experiments by A(ery and colleag!es pro(ed that D0A 2as the s!bstance that a non# pathogenic pne!mococc!s strain into a (ir!lent strain& Ans* G +ection ,&,&A Diffic!lty* %oderate $earning -b.ecti(e* Overview of Nucleic Acid unction 6& 7he method that +anger de(eloped for D0A D0A se8!encing !sing dideoxy n!cleotides is is called the method& Ans* " +ection ,&3&C Diffic!lty* Easy $earning -b.ecti(e* Nucleic Acid Se!uencing 9& :lasmids are small' circ!lar D0A molec!les that are !sed in & Ans* F +ection ,&5&A Diffic!lty* Easy $earning -b.ecti(e* Manipulating "NA ;& Certain plasmids contain genes that that confer resistance resistance to & Ans* I +ection ,&5&A Diffic!lty* Easy $earning -b.ecti(e* Manipulating "NA <& A small fragment of labeled D0A or 40A !sed in a hybridi=ation experiment experiment is called a & Ans* C +ection ,&5&B Diffic!lty* Easy $earning -b.ecti(e* Manipulating "NA 1>& After hybridi=ation' the fragment of interest can be detected by & Ans* +ection ,&5&B Diffic!lty* Easy $earning -b.ecti(e* Manipulating "NA
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Multiple Choice 11& 0!cleotides play a central role in li(ing li(ing organisms beca!se A) they mediate transport of energy 2ithin the cell& B) they are in(ol(ed in oxidation#red!ction reactions& C) they are in(ol(ed in intracell!lar intracell!lar signaling& D) they f!nction as b!ilding blocs for n!cleic acids& E) all of the abo(e Ans* E +ection ,&1 Diffic!lty* Easy $earning -b.ecti(e* Nucleotides
1/& ?hich of the follo2ing statements abo!t n!cleotides is false@ A) 0!cleotides mediate transport of energy 2ithin the cell& B) 0!cleotides are in(ol(ed in(ol(ed in oxidation#red!ction reactions& C) 0!cleotides store store genetic information& D) 0!cleotides are in(ol(ed in biosynthetic reactions& E) none of the abo(e Ans* C +ection ,&1 Diffic!lty* Easy $earning -b.ecti(e* Nucleotides
1,& ?hat gro!p is attached to the pyrimidine ring in thymine thymine and is not present in !racil@ A) ribose B) CH, C) 0H/ D) deoxyribose E) none of the abo(e Ans* B +ection ,&1 Diffic!lty* %oderate $earning -b.ecti(e* Nucleotides
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13& 7he molec!le sho2n in the diagram is a
A) B) C) D) E)
p!rine& din!cleotide& n!cleoside& n!cleotide& none of the abo(e
Ans* E +ection ,&1 Diffic!lty* Easy $earning -b.ecti(e* Nucleotides
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15& ?hat is the name of the molec!le sho2n in the the diagram@
A) B) C) D) E)
adenosine cytosine cytidine cytidine monophosphate !ridine
Ans* C +ection ,&1 Diffic!lty* Easy $earning -b.ecti(e* Nucleotides
16& 0!cleotides contain one or more phosphate gro!ps that are are !s!ally attached to the* A) C, or C5 atoms B) C, or C, atoms C) C5 or 0, atoms atoms D) C1 or 0, atoms E) none of the abo(e Ans* A +ection ,&1 Diffic!lty* %oderate $earning -b.ecti(e* Nucleotides
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19& A%: is !sed to refer to A) adenosine& B) adenylic acid& C) adenomethyl p!rine& D) adenine& E) all of the abo(e Ans2er* B +ection ,&1 Diffic!lty* Diffic!lt $earning -b.ecti(e* Nucleotides
1;& Inside o!r cells' free n!cleotides are almost al2ays associated 2ith & A) proteins B) cholesterol C) Cl co!nterions D) fatty acids E) %g/ co!nterions Ans2er* E +ection ,&1 Diffic!lty* %oderate $earning -b.ecti(e* Nucleotides
1<& H!mans typically recycle approximately of A7: each day& A) 5> g B) 5> mg C) 5> g D) 5> g E) 5>> g Ans2er* A +ection ,&1 Diffic!lty* Easy $earning -b.ecti(e* Nucleotides
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/>& 0!cleoside triphosphates are !sef!l for energy transfer beca!se the phosphoanhydride bonds are relati(ely & A) stable B) high energy C) biocompatible D) large E) lo2 energy Ans2er* B +ection ,&1 Diffic!lty* Easy $earning -b.ecti(e* Nucleotides
/1& 0!cleoside triphosphates carry energy in the form of A) glycosidic bonds B) phosphoester bonds C) phosphoanhydride bonds D) hydrogen bonds E) amide linages Ans2er* C +ection ,&1 Diffic!lty* %oderate $earning -b.ecti(e* Nucleotides
//& 7he ma.or carrier carrier of energy in the cell is* is* A) B) C) D)
adenosine monophosphate adenosine diphosphate adenosine triphosphate adenosine tetraphosphate
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/,& ?hich of the follo2ing n!cleotides contain energy rich bonds@ A) A7: B) 77: C) G7: D) C7: E) all of the abo(e Ans2er* E +ection ,&1 Diffic!lty* Easy $earning -b.ecti(e* Nucleotides
/3& ?hich of the follo2ing molec!les does not contain an energy rich phosphoanhydride bond@ A) AD: B) GD: C) A%: D) CD: E) all of the abo(e Ans2er* C +ection ,&1 Diffic!lty* Easy $earning -b.ecti(e* Nucleotides
/5& By con(ention' the se8!ence of n!cleotide resid!es in a n!cleic acid is 2ritten 2ritten starting 2ith the end& A) left to right , B) right to left , C) left to right 5 D) right to left ,
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/6& Chargaffs r!les state that* A) 7he amo!nt of g!anine pl!s cytosine in mammalian genomes (aries (aries from abo!t ,< to 36& B) D0A is al2ays do!ble stranded and 40A is al2ays single stranded& C) D0A contains t2o groo(es' a ma.or groo(e groo(e and a minor groo(e& D) In D0A the n!mber of adenine resid!es is identical to the n!mber of thymine resid!es and the n!mber of g!anine resid!es is identical to the n!mber of cytosine resid!es& E) D0A contains t2o strands strands that r!n in in opposite directions& Ans2er* D +ection ,&/&B Diffic!lty* Easy $earning -b.ecti(e* Introduction to Nucleic Acid Structure
/9& ?hich of the follo2ing bases pairs 2ith g!anine@
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/;& 7he ?atson and Cric model of a do!ble#helical str!ct!re for D0A 2as based' in part' on e(idence from A) 0%4 n!clear magnetic resonance) spectroscopy& B) I4 infrared) spectroscopy& C) atomic force microscopy& D) electron microscopy& E) #ray diffraction & Ans2er* E +ection ,&/&B Diffic!lty* Easy $earning -b.ecti(e* Introduction to Nucleic Acid Structure
/<& "no2ledge abo!t the ta!tomeric ta!tomeric forms of the bases of n!cleic acids is needed A) to !nderstand H#bonding H#bonding bet2een the complementary bases B) to !nderstand ho2 the bases are lined to ribose& C) to !nderstand ho2 bases are lined to deoxyribose& D) to !nderstand the ability of n!cleotides to act as energy carriers& E) to disting!ish the 5#end of a D0A strand from the ,#end& ,#end& Ans2er* A +ection ,&/&B Diffic!lty* Easy $earning -b.ecti(e* Introduction to Nucleic Acid Structure
,>& 0!cleotides in a D0A molec!le are lined together thro!gh A) glycosidic bonds& B) amide linages& C) hydrogen bonds& D) phosphoanhydride bonds& E) phosphodiester bonds&
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,1& A do!ble stranded D0A fragment contains 1/ adenine resid!es& Calc!late the percentage cytosine resid!es& A) 1/ B) /3 C) ,; D) 5> E) 9; Ans2er* C +ection ,&/&B Diffic!lty* %oderate $earning -b.ecti(e* Introduction to Nucleic Acid Structure
,/& ?hich of the follo2ing bases is not present in 40A@
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,,& 40A occ!rs primarily as single stranded molec!les that can gi(e rise to str!ct!res& A) diploid B) stem#loop C) parallel D) ta!tomeric E) haploid Ans2er* B +ection ,&/&C Diffic!lty* Easy $earning -b.ecti(e* Introduction to Nucleic Acid Structure
,3& In 1<33 A(ery' %ac$eod' and %cCarty did experiments 2ith Diplococc!s pne!moniae that pro(ed that A) D0A is do!ble stranded& B) D0A contains deoxyribose& C) D0A can be transcribed into 40A& D) D0A can permanently transform transform a non#pathogenic strain into a pathogenic strain& E) 7he str!ct!re of D0A pro(ides a mechanism for reliable replication& Ans2er* D +ection ,&,&A
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,6& It is impossible for 40A to to store genetic information' beca!se A) 40A does not base pair and therefore cannot be replicated& B) en=ymes cannot process genetic information information in the form form of 40A& C) 40A forms forms complex folded str!ct!res& str!ct!res& D) 40A is (ery !nstable& E) 0one of the abo(e ans2ers is correct 40A can store can store genetic information& Ans2er* E +ection ,&,&A Diffic!lty* Diffic!lt $earning -b.ecti(e* Overview of Nucleic Acid unction
,9& Genomic D0A is is ' res!lting in the prod!ction of & A) transcribed m40A B) translated t40A C) transcribed protein D) translated protein E) translated r40A Ans2er* A +ection ,&,&B Diffic!lty* %oderate
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,<& ?hich diagram depicts correctly a ribosome ribosome engaged in translation@
A&) a B&) b C&) c D&) d
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31& ?hich of the follo2ing D0A se8!ences is considered palindromic@ A) AAGC77 B) GAAC77 C) GAACAA D) AAGCAA E) AAG77C Ans* A +ection ,&3&A Diffic!lty* %oderate $earning -b.ecti(e* Nucleic Acid Se!uencing
3/& ?hich of the follo2ing D0A se8!ences in 2hich 0 is any n!cleic acid resid!e) resid!e) is palindromic@ A) A0AGC77 B) AA0GC77 C) AAG0C77 D) AAGC077 E) AAGC707 Ans* C +ection ,&3&A
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33& ?hich of the restriction en=ymes en=ymes listed in the table belo2 prod!ces prod!ces bl!nt#end fragments@
A) B) C) D) E)
En=yme
Clea(age site
Al!I HindIII BamHI Eco4I BglII
AGC7 AAGC77 GGA7CC GAA77C AGA7CA
Al!I BamHI BglII Eco4I HindIII
Ans2er* A +ection ,&3&A Diffic!lty* %oderate $earning -b.ecti(e* Nucleic Acid Se!uencing
35& ?hich of the en=ymes listed in the table belo2 prod!ce identical sticy ends@
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36& Gel electrophoresis generally separates n!cleic acids on the basis of A) shape& B) se8!ence& C) si=e& D) charge#density& E) none of the abo(e Ans* C +ection ,&3&B Diffic!lty* Easy $earning -b.ecti(e* Nucleic Acid Se!uencing
39& D0A se8!encing !sing the +anger method re8!ires
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3<& D0A se8!encing by the chain#termination method !tili=es !tili=es D0A polymerase I to mae a complementary copy of the target or template D0A molec!le& A reaction 2ith a /> bp template template and dideoxyadenosine n!cleotides as terminators res!lts in the prod!ction of a 5 bp fragment& Based on this res!lt' 2e can concl!de that the template contains A) a cytosine at position 5& B) a thymine at position 5& C) a cytosine at position 16& D) a thymine at position 16& E) a !racil at position 5& Ans2er* D +ection ,&3&C Diffic!lty* Diffic!lt $earning -b.ecti(e* Nucleic Acid Se!uencing
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5/& ?hich of the follo2ing concl!sions abo!t the the h!man genome is false@ false@ A) -nly abo!t ;> of the genome is transcribed to 40A& B) %ost of the proteins fo!nd in h!mans are are !ni8!e to (ertebrates& (ertebrates& C) 0early half of the h!man genome consists of repetiti(e D0A se8!ences& D) 7he genomes of any t2o people are liely to be <<&< identical E) 0one of the abo(e Ans* B +ection ,&3&D Diffic!lty* Diffic!lt $earning -b.ecti(e* Nucleic Acid Se!uencing
5,& Alterations in genetic information can be ca!sed by
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55& %!tations leading to changes that can be inherited by the next generation ha(e to be introd!ced into at the le(el& A) D0A B) r40A C) protein D) m40A E) t40A Ans2er* A +ection ,&3&E Diffic!lty* Easy $earning -b.ecti(e* Nucleic Acid Se!uencing
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5;& In molec!lar cloning' transformed transformed organisms m!st be identified& -ne common method for accomplishing this in(ol(es the incl!sion of in the plasmid& A) a restriction site B) a n!clease gene gene C) a deletion D) an origin of replication E) an antibiotic resistance gene Ans2er* E +ection ,&5&A Diffic!lty* %oderate $earning -b.ecti(e* Manipulating "NA
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61& In order to perform :C4' 2hich of the follo2ing follo2ing describes the reagents that m!st be incl!ded in the reaction mixt!re@ A) D0A fragment' primers flaning flaning the region of interest' d07:s' D0A polymerase B) D0A fragment' primers flaning the the region of interest' d07:s' dd07:+' D0A polymerase C) D0A fragment' one primer' d07:s' D0A D0A polymerase' D0A ligase
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63& A gene noco!t is A) a gene that has been inacti(ated or remo(ed from an organism& B) a dominant gene that knocks-out expression expression of other genes& C) a gene inserted in place of another gene& gene& D) a gene present on a JAC&
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Short Answer 66& 7his is the the str!ct!re of adenine&
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69& 7his is the the str!ct!re of cytosine&
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6;& Describe the str!ct!re of a D0A molec!le by listing 6 characteristics& Ans*
1& D0A forms a do!ble helix /& the t2o strands r!n anti#parallel ,& the s!gar is deoxyribose
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