Pro II Tutorial
Bioethanol from Starch Starch Process Pr ocess Simulation Simulation Tutorial Tutorial on PRO II/8.2 II/8.2
CHE 581 Washington Wa shington State University Benjamin Lant !uesong Li
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Pro II Tutorial
Bioethanol #rom Star$h Pro$ess Simulation Tutorial Tutorial on P%& II Introduction to the project: It is essential #or mo'ern so$iety to ensure a $lean( sustaina)le( se$ure an' a##or'a)le energy su**ly+ ,meri$a-s $urrent energy $hallenge is meeting the *roje$te' in$rease in energy 'eman' .hile 'e$reasing 'e*en'en$e on #oreign sour$es o# energy+ ,lthough *etroleum *rovi'es * rovi'es more energy #or the Unite' States than any other resour$e( 'omesti$ *etroleum reserves an' *ro'u$tion are very limite'+ ,s a result( im*orts $ontinue to gro. an' have rea$he' over /"0 o# the USs total *etroleum $onsum*tion+ , sustaina)le( rene.a)le( an' environmentally #rien'ly energy resour$e is the 'esira)le alternative+
Bioethanol is a li2ui' )io#uel .hi$h $an )e *ro'u$e' #rom several 'i##erent )iomass #ee'sto$3s an' $onversion te$hnologies+ Bioethanol is the most em*loye' li2ui' )io#uel( either as a #uel or as a gasoline enhan$er+ It is an attra$tive attra$tive alternative #uel )e$ause it is a rene.a)le( )io4)ase' resour$e an' it is oygenate' .hi$h *rovi'es the *otential to re'u$e *arti$ulate emission in $om*ression4ignition engines+ Bioethanol itsel# has a higher o$tane num)er( )roa'er #lamma)ility limits( higher #lame s*ee's an' higher heats o# va*oriation than gasoline+ These *ro*erties allo. #or a higher $om*ression ratio( shorter )urn time an' leaner )urn engine( .hi$h lea' to theoreti$al e##i$ien$y a'vantages over gasoline in an internal $om)ustion engine+ In summary( ethanol ma'e #rom )iomass has the greatest a'vantage .ith regar' to environment( e$onomi$s( an' in#rastru$ture+ General process of Bioethanol from Starch
Ethanol is $ommer$ially *ro'u$e' in one o# the t.o .ays6 .et mill an' 'ry mill *ro$ess+ The 'i##eren$e is that .et milling involves se*arating grain 3ernel into its $om*onent *arts( e+g+( germ( #i)er( *rotein( an' star$h( *rior to #ermentation( an' 'ry mill *ro$ess groun' the entire grain 3ernel into #lour( the star$h in the #lour is $onverte' into ethanol 'uring 'uring #erme #ermentat ntation ion *ro$es *ro$ess( s( emitt emitting ing $ar)on $ar)on 'ioi' 'ioi'ee an' 'istil 'istiller lers s grain+ grain+ 7rom 7rom $hemistry *oint o# vie.( the *ro$ess $an )e .ritten as #ollo.s6 Enymes east east 9C:H1"&5;n < nH=& 444> nC:H1=&: 444> =nCH?CH=&H< =nC&= @etaile' *ro$esses are as #ollo.s 9a#ter star$h4)ase' )iomass is $olle$te' an' 'elivere' to the ethanol *ro'u$tion *lant;6 1+ Pretreatment an' milling+ With 'ry mill *ro$ess( the grain is s$reene' to remove 'e)ris an' groun' into $ourse #lour+ The mille' grain then mie' .ith .ater to #orm a mash+ @uring .et mill *ro$ess( grain is soa3e' 9stee*e'; in .ater an' 'ilute sul#urous a$i' in or'er or'er to se*ara se*arate te grain grain into into its its many $om*onent $om*onent *arts+ *arts+ ,#ter ,#ter soa3in soa3ing( g( the slurry slurry is *ro$esse' through a series o# grin'ers( .ashing an' #iltering *ro$ess to se*arate germ( #i)er( an' gluten( to #orm mash $ontaine' mainly star$h an' .ater+ =+ Enymati$ hy'rolysis+ The mash #orme' at a)ove .ill un'ergo $oo3ing *ro$ess( at .hi$h *oint ammonia is a''e' a$ting as )oth *H $ontrol $ontrol an' nutrient nutrient to yeast( yeast( enymes enymes 9e+g+ al*ha4amylase; are a''e' to $onvert star$h into sim*le sugars( su$h as 'etrose+ 1
Pro II Tutorial
?+ Enymati$ hy'rolysis $ontinue'+ The mash is then *ro$esse' .ith higher tem*erature to re'u$e )a$teria levels( #ollo.e' )y *H an' tem*erature a'justment suita)le #or the se$on' enyme+ , se$on' enyme( usually glu$oamylase is then a''e' an' the miture is *um*e' into #ermentation tan3s+ A+ Simultaneous sa$$hari#i$ation #ermentation+ The glu$oamylase enyme )rea3s 'o.n the 'etrins to #orm sim*le sugar 9glu$ose; insi'e o# the #ermentation tan3s+ east is then a''e' into the tan3 to $onvert the sim*le sugar to ethanol an' $ar)on 'ioi'e+ 7ermentation results a miture that $ontains a)out 150 ethanol an' other materials( su$h as yeast( et$+ 5+ @istillation+ The #ermente' mash is then *um*e' into a multi4$olumn 'istillation system .here a''itional heat is a''e'+ The $olumns use the 'i##eren$e o# the )oiling *oint o# ethanol an' .ater to se*arate ethanol #rom mash+ Ethanol is in the stream usually $an )e in 50 *urity .ith .ater+ :+ @ehy'ration+ The 'istille' ethanol is then *assing through mole$ular sieve to *hysi$ally se*arate the remaining .ater+ This is )ase' on the 'i##erent mole$ular sie to *ro'u$e anhy'rous ethanol #or storage or usage+
Benefits of Using Model Simulation in Your Work
o'el simulation is the re$reation or re*ro'u$tion o# a *arti$ular *ro$ess using $om*uter so#t.are #or analysis an' #ore$asting *ur*oses+ Here are some )ene#its o# using mo'el simulation #or this *roje$t+ • • • • • • • •
,nti$i*ate *otential *ro)lems an' ris3s6 Su**ort D.hat i# Danalysis+ usti#i$ation #or *ro$ess im*rovement an' te$hnology a'o*tionFtrans#er Gno. *otential out$ome o# *ro$ess $on'itions Will have a )etter un'erstan'ing Training an' learning &*timie Bio*ro$ess enerate 2ui$3er $om*utational results Savings
Bioprocess Simulation Model
The *ro$ess mo'el is 'evelo*e' using P%& II 8+=+ Ho.ever( )e#ore $reating a mo'el in P%& II( the $onversion rate #rom star$h to glu$ose an' glu$ose to ethanol nee' to )e 'etermine'+ The $onversion rate #or star$h is )ase' on the #ollo.ing journal arti$le6 DParameter Estimation #or Simultaneous Sa$$hari#i$ation an' 7ermentation o# 7oo' Waste into Ethanol Using atla) Simulin3 )y %e)e$$a ,nne @avis+ The $onversion rate is )ase' on " gFl o# star$h that *ro'u$es 8" gFl o# glu$ose .hi$h e2uates to 80 =
Pro II Tutorial $onversion rate+ Jet( to #in' the $onversion rate #rom glu$ose to ethanol ,TL,B .ill )e use'+ This is 'one )y simulating the gro.th 3ineti$s o# ethanol *ro'u$tion #rom glu$ose using the rate e2uations liste' )elo.+ The initial $on$entration o# glu$ose is )ase' on the a)ove arti$le+ Optional See a**en'i , to generate )oth a numeri$al an' gra*hi$al solutions o# the $on$entration o# glu$ose( ethanol an' $ells as a #un$tion o# time using ,TL,B+ ou then $an $al$ulate the $onversion #orm glu$ose to ethanol+ The rate e2uations #or this *ro$ess are given )elo.6
dC S dt dC P dt dx dt
=
= r S = −L
µ ma5 s
Y XS 9 K S + s ;
= r P = −LY PX µ ma5 s
K S + s
µ ma5 s
K S + s
+ mS K x
+ m P K x
x
Assumptions Made to Generate a rocess Model • • • •
Stea'y state Star$h is $om*ose' o# 8"" glu$ose units I'eal $on'itions Even though the $onversion o# star$h an' glu$ose is not 1""0( the $om*onents ?
Pro II Tutorial .ere $onsi'ere' insigni#i$ant as *ro'u$t streams+
A
Pro II Tutorial
Using ro II !"#: roduction of $thanol from Starch art %: &reating a ne' flo' sheet Ste* 16 &*en PROII 8.2 i$on )y $li$3ing start F ,ll Programs F SISCI F Pro II 8+= FP%&II 8+=+ The .el$ome .in'o. tells .hat o*erations are re2uire' )y $olor 'esignation6 %e' )or'ers mean 'ata an' a$tion re2uire'+ reen )or'er means user may overri'e this #iel'+ Blues )or'ers mean that the 'ata you #ille' in the entry #iel'+ ello. )or'ers mean that you su**lie' 'ata outsi'e the normal range o# values #or a #iel'+ Cli$3 &G on Welcome to PRO/II
Ste* =6 &*en ne. #lo. sheet+ By $li$3ing
i$on+
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Pro II Tutorial
Ste* ?6 Save *roje$t )y $li$3ing $li$3 Save.
i$on+ Jame the #ile Bioethanol from Starch an'
(O)$: &lick Sa*e after each A+) of this tutorial is done"
Ste* A6 To $hange units o# measurements there are t.o o*tions+ Option A: Changes units #or entire P7@+
To set the units that .ill )e use' through out the P7@ either sele$t DUnits of Measure un'er the DIn*ut ta) or $li$3 the i$on+ Change )emperature to &elsius( ressure to ound,inch-#.a/s01 Weight.'t0 to 2ilogram1 3i4uid 5olume to Meter-61 an' 5apor 5olume to Meter-6" Cli$3 OK.
Option B6 Change units #or ea$h *ro$ess unit 9 See Part =, )elo. Ste* 15;
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Pro II Tutorial
art #A: Setting up li/rar7 and h7pothetical components Ste* 16 ,'' ne. $om*onent )y *ressing on
i$on+
$ntering 3i/rar7 components: Ty*e D$thanol in the Component Selection .in'o.+ Cli$3 Add + %e*eat the *ro$ess #or DWater an' D&O#+
$ntering 87pothetical components: Enter t.o $om*onents that are not in the system )y $li$3ing on Uer!defined ta) in the D&omponent Section .in'o.+ Ty*e DStarch in the D&omponent (ame )o+ Cli$3 DAdd+ %e*eat #or DGlucose +
(O)$: You should see starch and glucose under the 9&omponents to /e Added
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Pro II Tutorial ou .ill get an alert .hen you *ress &G( .arning that *ro*erties o# the ne.ly a''e' $om*onents must )e su**lie'( .hi$h .e .ill ta3e $are o# net+
Ste* =6 Cli$3 OK t.i$e (O)$: You should see fi*e components in the list: $)8A(O31 WA)$+1 &O #1 S)A+&81 and G3U&OS$"
Ste* ?6 Cli$3 OK Ste* A6 Cli$3 on $om*onent *ro*erties i$on Ste* 56 Cli$3 on D;ill from structure in the D)hermoph7sical properties se$tion+ Ste* :6 Sele$t Star$h #rom DA*aila/le &omponents an' Cli$3 "ove to D&omponents to /e ;illed + %e*eat #or Glucose+
Ste* /6 Cli$3 OK Ste* 86 Cli$3 U#I$AC Str%ct%re Ste* 6 Cli$3 U#I$AC Str%ct%re #or Starch in the $om*onent se$tion+ 8
Pro II Tutorial
Ste* 1"6 Enter $om*onents o# the ST,%CH #rom the ta)le )elo.+ Jote star$h is a *olymer o# @4glu$ose an' has the #ollo.ing stru$ture an' .e .ant to mimi$ it+ Basi$ally this is a *olymer o# altose mole$ules .e $all amylose+ Hy'rolysis o# amylose ta3es *la$e .ith enymes that )rea3 'o.n or hy'rolye star$h into the $onstituent sugars+ These enymes are 3no.n as amylases an' su$h M4amylases are #oun' in *lants an' in animals+ 7or eam*le( human saliva is ri$h in amylase( an' the *an$reas also se$retes the enyme+ These *olymers ty*i$ally have very high )ut in'e#inite mole$ular .eights o# 1"(""" or so( an' $an )e )ro3en 'o.n through $hemi$al *ul*ing to 5"" N =""" W mole$ules+ These un'ergo li2ue#a$tion .ith amylase to maltose .hi$h is then $onverte' to glu$ose .ith maltase+ The #ollo.ing a**ears to )e )ase' on a W o# 8"" re*eating glu$ose units( i+e+ 8"" al$ohols o# the CH=&H ty*e( 1:"" al$ohols o# the NCH&H ty*e( 1:"" ethers 9t.o *er glu$ose( one on ea$h en';( an' =A"" N CH *ara##in grou*s 9i+e+ A NCH grou*s *er glu$ose;+ a" /" c" d" e"
Un'er &ategor7 sele$t DAlcohols Un'er DGroup sele$t <#<< Cli$3 ,'' rou* Un'er D(um/er $olumn $hange D% to D %=<< %e*eat ste*s 9a4'; until all the values have )een in*utte'+
&ategor7 Group (um/er ,l$ohols "="" 1:"" ,l$ohols "=// 8"" Ethers ":"= 1:"" Para##ins ""= =A"" (O)$: )he 'indo' /elo' should look similar after the completion of the ta/le
Pro II Tutorial
Ste* 116 Cli$3 OK Ste* 1=6 %e*eat Ste* 1" an' Ste* 11 #or G3U&OS$ )y using the values in the ta)le )elo.+ Jote glu$ose has the #ollo.ing stru$ture an' .e .ant to mimi$ it .ith su)4mole$ular $ategories+ &ategor7 ,l$ohols ,l$ohols ,l'ehy'e Para##ins
Group "="" "=1A "?"" ""=
(um/er A 1 1 ?
These $orres*on' to the #ollo.ing grou*s6
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Pro II Tutorial In reality glu$ose in solution #orms a ring stru$ture 9+80 .ith only "+"=0 in the straight $hain #orm; so .e really .ant to $orre$t our su)4 mole$ular $om*onents )ase' on the #ollo.ing stru$ture+ We .oul' sim*ly re*la$e the al'ehy'e grou* 9O&; stru$ture .ith an ether stru$ture 94CH4&4C4;( *ro)a)ly .ith Jo+ :"= or *erha*s Jo+ :88+
Ste* 1?6 Cli$3 OK Ste* 1A6 Cli$3 ;i>ed in D)hermoph7sical properties se$tion+ Enter the $om*onent *ro*erties o# S)A+&8 an' G3U&OS$ #rom the ta)le )elo.+ &omponent
Star$h lu$ose
Molecular Weight .g,mol0
1=:"" 18"
Standard 3i4uid ?ensit7 .kg,m60
(ormal Boiling oint . o& 0
15"" 15A"
=5" 15"
11
Pro II Tutorial
Ste* 156 Cli$3 OK t.i$e+ Important (ote: The other o*tion to $hange units is $li$3 the value un'er DStandard 3i4uid ?ensit7 + Then $li$3 UOM 9Unit o# easurement;( #inally sele$t 'esire' units : kg,m6+
Ste* 1:6 Sele$t the thermo'ynami$ *a$3age #or the *ro$ess $al$ulations )y either sele$ting Inp%t ta) on the menu )ar an' then $li$3 )hermod7namic ?ata or *ress the i$on on the tool )ar+ Ste* 1/6 Un'er D&ategor7 sele$t Most &ommonl7 Used Ste* 186 Un'er Drimar7 Method Sele$t (+)3 Ste* 16 Cli$3 Add
1=
Pro II Tutorial
Ste* ="6 Cli$3 O2 t.i$e
art #B: ?efining +eactions Ste* 16 Cli$3 Inp%t 9menu )ar; the sele$t Reaction &ata Ste* =6 Un'er D+eaction Set (ame + Ty*e DS)A+&8( an' then give a 'es$ri*tion+ %e*eat #or $)8A(O3+ See s$reen )elo.+
Ste* ?6 Cli$3 $nter ?ata #or ST,%CH rea$tion+ Ste* A6 Cli$3 %e' Bo un'er DJame+ Enter DStar$h an' $li$3 %e' highlight letters D%ea$tants O Pro'u$ts+ Ste* 56 Ty*e 9!<< in the .hite )o net to H="+ Ty*e 9% #or S)A+&8( an' 9!<< #or G3U&OS$+ Jote6 Then )alan$e the rea$tion6 Star$h < 8"" H=" O 8"" lu$ose
1?
Pro II Tutorial Ste* :6 Cli$3 OK t.i$e Ste* /6 %e*eat ste*s ?4:( e$e*t ty*e DETH,J&L an' in STEP 5( ty*e 9% in the .hite )o net to G3U&OS$+ Ty*e 9# #or =O( an' 9# #or &O#+ Jote6 Then )alan$e the rea$tion6 lu$ose O = C=H:& < = C&=
art 6: &reating a rocess ;lo' ?iagram .;?0 @ra. P7@ that $onsists o# t.o $onversion rea$tors( sim*le heat e$hanger an' a 'istillation $olumn $onne$te' .ith in*ut an' out*ut streams as sho.n in #igure )ello.+
Ste* 16 Cli$3 'ie( / Palette / P$& 1A
Pro II Tutorial
art @A: &reating &on*ersion +eactor .+%0 Ste* 16 Cli$3 on &on*ersion +eactor +% #rom the P7@ *allet Ste* =6 Cli$3 Steams #rom the P@7 *allet Ste* ?6 Conne$t inlet stream )y 'ragging hea' o# arro. to the le#t si'e o# the rea$tor+ %e*eat the *ro$ess #or another inlet stream 9total o# t.o inlet streams;+ Ste* A6 %e*eat the *ro$ess( e$e*t use only one stream 9outlet; an' 'rag #rom the tail en' o# the arro. to the right si'e o# the rea$tor+
15
Pro II Tutorial Ste* 56 @ou)le $li$3 the in*ut stream S% an' rename DWA)$+ + Then $li$3 on ;lo'rate and &omposition un'er D&omposition ?efined se$tion+ Ste* :6 Cli$3 )otal ;luid ;lo'rate + In*ut D%<<< kgmole,hr ( an' then un'er the D&omposition Mole se$tion( in*ut D%"<< Mole #or WA)$+ + Ste* /6 Cli$3 on O2 +
Ste* 86 Un'er D)hermal &ondition ( ma3e D;irst Specification to )e Tem*erature( an' in*ut D# o& DSe$on' S*e$i#i$ation to )e Pressure an' in*ut D%@"C psia+
Ste* 6 Cli$3 O2 1:
Pro II Tutorial Ste* 1"6 @ou)le $li$3 stream S# an' re*eat ste* 54( e$e*t stream name DS)A+&8( Total #lo. rate D% kgmole,hr( Com*osition #or star$h D% mole+ Ste* 116 @ou)le $li$3 stream S6( an' rename DGlucose + Ste* 1=6 Cli$3 on the rea$tor +% an' un'er D+eaction Set (ame $li$3 S)A+&8+ Ty*e 9%!<<& #or D;i>ed )emperature + Cli$3 on D$>tent of +eaction an' un'er DBase &omponent sele$t Starch( an' in*ut 9<"!D un'er con*ersion coefficient A +
Ste* 1?6 Cli$3 O2 t.i$e Ste* 1A6 Cli$3 +un on the tool )ar
(ote: If reactor is /lue then process to ne>t step other'ise repeat A+): 6A
1/
Pro II Tutorial
art @B: &reating &on*ersion +eactor .+#0 Ste* 16 Sele$t another $onversion rea$tor #rom the P7@ *allet( then $onne$t G3U&OS$ stream to +#+ Ste* =6 Sele$t t.o more stream #rom the P@7 *allet an' $onne$t on the right si'e o# %= 9outlet streams;+ %ename u**er outlet stream D&O# an' lo.er stream D$)8A(O3+ Ste* ?6 @ou)le $li$3 +#" Un'er D%ea$tion Set Jame $li$3 $)8A(O3+ Ty*e 96<& #or D;i>ed )emperature + Cli$3 on D$>tent of +eaction an' un'er DBase &omponent sele$t GlU&OS$( an' in*ut 9<"=!C un'er $onversion $oe##i$ient ,+ Ste* A6 Cli$3 on roduct hases + Change *hases a$$or'ing to the s$reen )ello.+ Un'er Dhases sele$t 5apor #or &O# an' sele$t 3i4uid #or $)8A(O3+
Ste* 56 Cli$3 O2 t.i$e+ Ste* :6 Cli$3 %un
i$on
(ote: If reactor is /lue then process to ne>t step other'ise repeat A+):@B
art : &reating Simple 8eat $>changer Ste* 16 Sele$t DSim*le H! #rom the P7@ *allet+ Ste* =6 Conne$t Ethanol stream #rom D%= to le#t si'e o# heat e$hanger 9H!;+ %ename heat e$hanger to 98E%" Then $onne$t one stream on the right han' si'e o# the H! 9rename 9;$$?; Ste* ?6 @ou)le Cli$3 8E%( In the heat e$hanger $li$3 Specification ( then sele$t D8ot roduct temperature an' in*ut @C o& in the value )o #or tem*erature+ 18
Pro II Tutorial
Ste* A6 Cli$3 O2 t.i$e Ste* 56 Cli$3 %un
i$on
(ote: If reactor is /lue then process to ne>t step other'ise repeat A+):
art =: &reating ?istillation &olumn Ste* 16 Sele$t D?istillation #rom P7@ *allet+ Ste* =6 In*ut 9! in the .hite )o #or num)er o# trays( then $li$3 O2
Ste* ?6 Conne$t D;$$? stream to le#t si'e o# 'istillation $olumn .here it rea's D @ an' 'ra. one outlet +O?U&) stream #or the to* o# $olumn an' one outlet WAS)$ stream #or )ottom o# $olumn+ See )elo.
1
Pro II Tutorial
Ste* A6 @ou)le $li$3 ?istillation column an' $li$3 ressure rofile Ste* 56 In*ut D%@"C *isa #or to* tray *ressure( then $li$3 O2 Ste* :6 Cli$3 ;eeds and roducts" Enter 9@ in the .hite )o un'er Dtra7 an' then un'er D+ate in*ut D%%D= 3g4molFhr #or D +O?U&)+ In*ut D6! 3g4molFhr #or DWAS)$+
="
Pro II Tutorial Ste* /6 Cli$3 O2" Then $li$3 Yes" Ste* 86 Cli$3 erformance Specifications1 then un'er DSpecifications se$tion 1 D&O3%S$&% $li$3 the #irst )lue *arameter se$tion $hange DStream, Unit to Stream an' DStream (ame to Waste+ Cli$3 O2 + See s$reen )elo.
Ste* 6 $li$3 the se$on' )lue *arameter se$tion( $hange value to 9#<<+ Cli$3 O2 +
Ste* 1"6 Cli$3 &ondenser1 then in*ut 9<<& in the DTem*erature Estimate .hite )o+ Ste* 116 Cli$3 O2
=1
Pro II Tutorial Ste* 1=6 Cli$3 Initial $stimates1 Then $hange the tem*eratures as seen )elo.+
Ste* 1=6 Cli$3 O2 t.i$e Ste* 1?6 Ste* 56 Cli$3 %un
i$on
(ote: If reactor is /lue then procede to ne>t step other'ise repeat A+) ="
==
Pro II Tutorial
art C: +esults )WO O)OI(S &PTI&J ,6
Ste* 16 Cli$3 Star$h
on the tool )ar to generate a re*ort an' save DBioethanol #rom
Ste* =6 Save the #ile &PTI&J B6 Ste* 16 Cli$3 Output1 sele$t DStream *ro*erty Ta)le Ste* =6 @rag green )o )elo. the P7@ an' 'ou)le $li$3 the green )o+ Ste* ?6 Sele$t DStream Summar7 un'er DPro*erty List to )e use'( then $li$3 Add All un'er DStream Sele$tion se$tion+ Ste* A6 Cli$3 O2 Ste* 56 Save the #ile
(ote: Your process should look like the one sho'n /elo'"
=?
Pro II Tutorial
,PPEJ@I! , MA)3AB Background There are t.o main .in'o.s that atLa) uses+ The #irst .in'o. that *o*s u* .hen atLa) o*ens is the $omman' .in'o.+ This is .here the main #un$tions in atla) are run+ The se$on' .in'o. that atla) uses is $alle' the 4#ile e'itor+ This is .here the S$ri*t #ile or 4miles are $reate'+ Ho.ever( this tutorial .ill )e very )asi$ an' .ill ta3e sho. ste*4)y4ste* to generate a numeri$al an' gra*hi$al solution to #in' the $onversion #rom glu$ose to ethanol+ MA)3AB rogram
Ste* 16 &*en u* atla)+ &reate Mfile .no' called Scriptfile0
Ste* =6 Cli$3 7ileQJe.QS$ri*t or ty*e Ctrl
=A
Pro II Tutorial Ste* 1"6 you $an *la$e the 'ata into an ,SCII out*ut #ile )y ty*ing the $ omman'6 >> save Cornstar$hRtoREt&HR'ata+out P N,SCII+ ou .ill no. see a Cornstar$hRtoREt&HR'ata+out a**ear in the Current #ol'er .hi$h you $an rename )y right $li$3ing on the #ile+ ou .ill no. see #or eam*le6
Ste* 116 ou $an )ring that ,SCII #ile into E$el as 'elimite' 'ata )y6 Cli$3ing on D@ata ta) sele$ting D7rom Tet 7in' the #ile in a given #ol'er 9you .ill nee' to ma3e sure D,ll 7iles is sele$te' $li$3 on the DCornstar$hRtoREt&HR'ata+out #ile an' *ress im*ort+ 7ollo. the 'ire$tions #or 7ie' or Ta) 'elineate' 'ata+
ou $an no. *ro'u$e a *lot o# the 'ata in E$el .hi$h $an easily )e mo'i#ie' to loo3 something li3e this+
=5
Pro II Tutorial Use the Comman's in E$el on the Layout ta) to get the gra*h the .ay you .ant it+
We $an see #rom the 'ata that Ethanol .ill not rea$h the $riti$al an' toi$ level o# 15" gFL given the initial glu$ose level+ &ther simulations $an ) e 'one )eginning .ith higher glu$ose levels an' using ono' $ell gro.th 3ineti$ e*ressions that in$lu'e *ro'u$t inhi)ition terms su$h as the t.o o*tions )elo.6 n
C p µ s x 1 = ma5 − + K s C p ( ma5 S or K µ s dx p = ma5 x dt K S + s K p + C p dx dt
,TL,B 47ILE416 Ethanol+m % The production of Ethanol from Glucose % Given initial conditions and solving for three ODE's simultaneously, this program will plot % the concentration of Glucose, Ethanol, and Biomass. The plot will give % the maimum ethanol produced !ases on the su!strate "glucose#. clear$ clc$ % o &nitial su!strate concentration "g()# % Eo &nitial ethanol concentration "g()# % *o &nitial !iomass concentration "g()# o + -$ Eo + -$ *o + $ initial+ /o Eo *o0$ % intial conditions of glucose, ethanol, and !iomass tspan + -11 23.2$ /t,40 + ode32"5dydt6,tspan,initial#$ 7+ /t, 40$ disp "7#$ plot"t, 4"1,#,t,4"1,8#,'&.',t,4"1,6#,'&&'#, la!el "'t'#
,TL,B 47ILE416 'y't?+m function 4prime + dydt6 "t,4# %9s &u!strate constant "g()# %mu &:aimum growth rate "g()# %4s &4ield on su!strate "g(g# %4p &4ield on product "g(g# %ms &u!strate maintenance rate constant " g su!strate(g !iomass(hr # %mp &7roduct maintenance rate constant " g product(g !iomass(hr #
9s + -.-82$
=:
Pro II Tutorial mu + .-32$ 4s + -.2$ 4p + 2.66$ ms + -.-6;$ mp + -$ %
eros"6,#$ 4prime "#+ &""mu?4"##("4s?"9s @ 4"###@ ms#?4"6#$ 4prime "8#+ "4p?"mu?4"##("9s @ 4"##@ mp#?4"6#$ 4prime "6#+"mu?4"##("9s @ 4"##?4"6#$
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