P700 Service Manual

Pictus 700

CLINICAL ANALYZER

SERVICE MANUAL

Disclaimer This manual is intended to be a reference guide to assist atrained service engineer on troubleshooting and repair the autoanalyzer. Information provided here is offered “as is”, it could have either typographical error and / or technical inaccuracies. Every people assumes risk and liability for use of this information and company reserves its right to do future additions, omissions or modifications without prior advice. 1 . Pictus 700 Service Manual Rev.3

Table of Content Table of Content 1. Introduction 1.1. 1.2. 1.3. 1.4.

2.

3.

How to use this manual Icons description Advices for service personnel Nomenclature and naming conventions

System Description

7 7 9 9

12

2.1. 2.2.

Instrument Architecture Detail of components

12 13

2.3. 2.4. 2.5. 2.6. 2.7. 2.8.

Device parameters handling Motor controller boards Clot detector system Electronic scale for DI water and waste bottle. User operation of system modules Operation of manual movements

14 15 15 16 20 21

Troubleshooting 3.1.

System tests

26 26

3.1.1. 3.1.2. 3.1.3. 3.1.4. 3.1.5. 3.1.6. 3.1.7. 3.1.8. 3.1.9. 3.1.10.

Temperature Stray light Noise Stability Tip Pump Level detection Washer hydraulics Washer Dilution Photometer linearity

26 26 26 27 27 27 27 28 28 28

3.1.11. 3.1.12. 3.1.13.

Diluter linearity Chemistry analysis Clot detector

28 28 28

3.2. 3.3. 3.4.

Visible faults Measurement inconsistencies Verification and diagnostic procedures

3.4.1. 3.4.2. 3.4.3. 3.4.4. 3.4.5. 3.4.6. 3.5.

3.6.

Standard troubleshooting procedure Reload instrument parameters being offline General Voltage checking Instrument general status check Movement test Belt tension measurement and value settings

Protocol Overview

3.5.1. 3.5.2. 3.5.3. 3.5.4. 4.

2 7

Packet tracking C1 Protocol C2 Protocol Protocol layers

Understanding errors.log

Mechanical Calibrations

4.1. 4.2. 4.3. 4.4.

Photometer Arm and Reaction Tray Washer Arm and Washing Station

29 29 33

33 33 34 35 35 36 37

38 38 39 39 40

44 44 44 45 47

2 . Pictus 700 Service Manual Rev.3

4.5. 4.6. 4.7. 4.8. 4.9. 4.10. 4.11. 4.12.

5.

Preventive Maintenance 5.1. 5.2. 5.3. 5.4. 5.5. 5.6. 5.7.

6.

Arm and Sample Arm and Reagent Tray Sample Tray Reagent Tray Bar Code Reader ISE Module Photometer Calibration Sample sector definition Half-Yearly maintenance kit. Yearly maintenance kit. ISE Half-Yearly maintenance kit Fan filters cleaning Lubrication procedure Tubing Sets 12 month ISE Medica

Maintenance Schedule Photometer 6.1.

Verification and diagnostic

6.1.1. 6.1.2. 6.1.3. 6.2.

7.

Maintenance

6.2.1. 6.2.2. 6.2.3. 6.2.4. 6.2.5. Arm 7.1.

8.

Probe dispensing pump check Probe robotics check Probe level sensingcheck Mixer checking

Maintenance

53 53 53 55 55 56 61 63

63 64 64

64 64 65 66

66 66 67 68 71 74 74

74 74 74 75 76

7.2.1. Probe replacement 76 7.2.2. Removal of complete arm assembly 77 7.2.3. Vertical arm belt replacement and tension belt adjustment 78 7.2.4. Horizontal belt replacement and tension belt adjustment 80 7.2.5. Adjust and verify probe optical collision sensor (M400-P317 Head board) 83 Reaction Tray 85 8.1.


8.1.1. 8.2.

9.

Photometer cleaning Adjustment of filter delays Photometer calibration Lamp alignment & beam splitter calibration Halogen lamp replacement


7.1.1. 7.1.2. 7.1.3. 7.1.4. 7.2.

Lamp voltage stability check Filter wheel calibration check Photometer and lamp alignment check

48 49 50 50 50 51 52 52

Tray movement check

Maintenance

8.2.1. Complete removal of reaction tray 8.2.2. Removal and replacement of reaction tray heater 8.2.3. Removal and replacement of reaction trayfan 8.2.4. Reaction tray belt replacement and tension belt adjustment Reagent Tray 9.1.


9.1.1. 9.2.

Tray movement check

Maintenance

9.2.1.

Removal of complete reagent cooler assembly

85

85 85

85 86 86 86 88 88

88 88

88 3

. Pictus 700 Service Manual Rev.3

9.2.2. Removal of Peltier thermoelectric devices 9.2.3. Access to reagent verification sensor 9.2.4. Reagent tray belt replacement and tension belt adjustment 9.2.5. Cooler heating fans replacement 9.2.6. Procedure for reagent fridge temperature sensor replacement. 9.2.7. New reagent tray upgrade 10. Sample tray 10.1.


10.1.1. 10.2.

Tray movement check

Maintenance

10.2.1. Sample tray belt replacementand tension belt adjustment 11. Washer 11.1. 11.2.

Troubleshooting Verification and diagnostic

11.2.1. 11.2.2. 11.2.3. 11.3.

Maintenance

11.3.1. 11.3.2. 11.3.3. 11.3.4. 11.3.5. 11.3.6. 11.3.7. 11.3.8. 12. Diluter 12.1.

Diluter valve replacement Syringe diluter replacement Special replacement procedure for Diluter

JS ISE Troubleshooting Maintenance

13.2.1. 13.2.2. 13.2.3. 13.2.4. 13.2.5. 13.2.6. 13.2.7. 13.2.8. 13.2.9. 13.3.

Diluter belt check

Maintenance

12.2.1. 12.2.2. 12.2.3. 13. ISE 13.1. 13.2.

Removal of complete washer Adjust washer collision sensitivity Washer belt replacement and tension belt adjustment Drying block replacement Peristaltic pump tubing replacement Peristaltic pump rotor replacement Diaphragm for drying and water pump replacement Washer pumps filter replacement / cleaning


12.1.1. 12.2.

Washer hydraulics check Dispensing hydraulics check Washer mechanical check

Electrode removal or cleaning Pinch valve unclogging Pump tubing replacement Electrode recovery Sodium electrode conditioning Pump pressure adjustment Pinch valve tubing replacement ISE pack removal ISE pack installation

ISE MEDICA Module

13.3.1. Overview 13.3.2. ISE Installed in the instrument 13.3.3. Electrodes 13.3.4. Fluid Management 13.3.4.1. Calibrant A 13.3.4.2. Calibrant B

96 96 96 97 98 103 105 105

105 105

105 107 107 107

107 107 108 108

108 109 109 110 111 113 115 119 121 121

121 121

121 122 124 125 125 126

126 128 129 129 130 130 131 132 133 134

134 135 135 136 137 137 4


13.3.4.3. 13.3.4.4.

Cleaning Solution MEDICA Urine Diluent.

13.3.5. Test Ranges 13.3.6. ISE Pack Installation 13.3.7. ISE Pack Removal 13.3.8. MEDICA Operational Parameters 13.3.9. Replace electrodes procedure 13.3.10. Shutdown Procedure: Preparing the ISE Module for Storage 13.3.11. Manual commands 146 13.3.12. Operation commands 13.3.13. Installation Kit ISE Medica (PN:KITISE40CMED) 1. Fix the module A with the screws (1). 13.3.14. Troubleshooting 13.3.14.1. Overview 13.3.14.2. Communication Errors 13.3.14.3. Fluid Delivery 13.3.14.4. Electrode Stability 13.3.14.5. Troubleshooting Guide 14. Electronics 14.1.

Maintenance

137 137 138 138 140 142 143 143 145 146 148 150 151 153 154 154 154 155 155 159 159

14.1.1.

Special replacement procedure for Head board (M400- P299 or M400-P317) 159 14.1.2. SM, FW, and Temperature controller board replacement 160 14.1.3. CPU board replacement 161 14.1.4. Procedure to replace PL400242W stepper motor controller board for PL400313W microstepper motor controller board 161 14.1.5. Removal of preamplifier 162 15.15.1.Housing Maintenance

163 163

15.1.1. Instrument cover installation procedure 16. Charts and diagrams

163 165

16.1. 16.2.

17. 18.

General parameters Complementary information

18.1. 18.2. 18.3.

19.

Location of belts and sensors ISE Hydraulics schematic.

Identification of system leds Computer program folder contents Main menu user messages

APPENDIX

19.1. 19.2.

Glossary Technical specifications

166 169 169

170 172 172 173 174

176 176 179



1. 1.1.

Introduction How to use this manual

This document is grouped into five major sections containing different technical information: General Data Section I contains information on manual usage, product overview, accident prevention symbols and instructions, and theory of operation. Block diagrams Section II contains charts, diagrams, and graphical information to complete the information provided on previous chapter. Verification and diagnostic procedures Section III contains details on verification, checks, tests, adjustments, and calibrations, required to verify instrument operation and troubleshooting. Adjust and replacement procedures Section IV contains replacement procedures and required adjust activities. Apendix Section V provides a brief glossary, the spare parts code reference and technical specifications, and instrument block diagrams.

1.2.

Icons description Possible injury may result from allowing part(s) of your body to enter the range of movement of the following during instrument operation.

Potential Biohazard - Avoid contact with tips of probes. - Clean spills of potentially infectious materials as stated by the biosafety practices. - Consider all specimens, reagents, controls, etc., containing human blood and surfaces or components that have come into contact with human blood; and the above materials as potentially infectious. - Wear gloves, lab coats, and safety glasses. - Avoid contact with skin and eyes. In case of contact with skin or eyes, flush with water at least 15 minutes. Immediately seek medical attention. 7 . Pictus 700 Service Manual Rev.3

Class 1 Laser Product Warns against direct viewing into the light beam of barcode reader.

Electrical Shock Hazard To protect against electrical shock, use only approved power cords and electrical accessories such as those supplied with the Analyzer. Connect power cords only to properly grounded outlets. If water or reagents splash on the Analyzer, immediately power off the Analyzer at the Main Power Switch. Failure to power off the Analyzer may result in electrical shock or fire. Electrostatic Sensitivity Use caution when handling electronic components or devices sensitive to electrostatic discharge. - Turn off instrument before connecting or disconnecting any board to avoid circuitry damage. Hot Surface Warns against possible burns from hot objects or surfaces such the reaction tray heater. To prevent heater harm do not use the instrument with the reaction cover removed. Turn the instrument off for 15 minutes to before lamp replacement. – –

In vitro Diagnostics

Original manufacturer

Representative in theEC


1.3.

Advices for service personnel

The following comments prevents problems for both user and service: •

•

•

•

•

•

•

Care should be taken when installing new parts on the Review the special replacement procedures listed on adjust and replacement procedures, section III, for details.

Never install “screen savers” on autoanalyzer computer nor enable energysaving options from both BIOS or operating system (hardisk or display shut down) because they might interfer with normal software operation. Check computer and / or operating system manual for further details. Never connect instrument on.

/

disconnect

•

1.4.

RS-232

instrument-computer cable

Autoanalyzer turn on / off sequence is: Turn on: 1) Autoanalyzer 2) Printer and others Turn off: 1) Computer 2) Printer and others

with

the

3) Computer 3) Autoanalyzer

It is not recommended to touch the lamp bulb nor optical filters. In case of accidental touching, bulb should be immediately cleaned with a tissue and alcohol before turning instrument on. It is not recommended to keep the cover unnecessarily open since this will overload the reaction chamber heater reducing its useful life. It is not recommended to use the instrument without priming the hydraulics, if bubbles are observed at inlet tubbing, please purge with Maintenance > operations > hydraulic > system flush

•

instrument.

.

It is necessary to blank the reaction tray after replacing the reaction cuvettes, this resets the dirty-cuvette detection algorithm. All the instrument reactions are performed at 37oC any other value introduced in the method is only for reference purposes.

Nomenclature and naming conventions

1. Indication to access and indentify menus in this manual •

Bold-Italics is used show (E.g. a sequence of > menus, options or sections to font perform certainto tasks. Maintenance Operations > Hydraulic > System flush ).


•

When a parameter is mentioned, Bold-Italics is used to show required parameter locating path, parameter itself is written in bold only. (E.g. Maintenance > Pa Port number ).

rameters > Softw

are > Ge neral > Comm unication

>

2. Printed circuit board identification: a) Printed board identification codes (bare board without components) M

40

X

-P218

↓

↓

↓

Instrument Code

Board Version

Board Code

Instrument code is a two digits number defining srcinal target design for the board (40 means board srcinally designed for 4000 instrument). Next digit indicates board version, digits on the right of P shows the specific board code.

b) Catalog numbers (ordering code) of boards with components PL

40

X

F

218

↓

↓

↓

↓

Instrument Code

Board Version

Function code

Board Code

Coding is similar to board identification codes but there is an additional function code components on which board. defines specific


This code is required to order a board from manufacturer, because some functions are done by identical boards with differents components (e.g. generic motor controller or filterwheel motor controller).

3. Pin 1 mark: is used to identify the first pin in connectors, notice white dot on serigraphy face. In case of single-in-line connectors, adjacent pins are increasing numbers. For dual in-line connectors, first pin is dotted, second is the opposite, third is nextopposite and so on.

• 2

3 4

5 6

7 8

9 10

•

2

3

4

5

4. Flat wire connections: All flat wire must be connected to match first connector pin to marked wire (red or black).


2.

System Description

Pictus 700 is a reliable in vitro diagnostic chemistry analyzer for automati c testing of routine clinical chemistry tests and electrolytes. Instrument is controlled by a PC workstation with graphical user friendly interface. Software provides total control over the analyzing process and gives easy access to advanced statistical functions and reports.

2.1. Instrument Architecture Pictus 700Front is composed a set robotics and dispensing devices in twoto channels, and Back.byThis arrangement provides both speed anddivided redundance ensure backup mode operation. Each channel includes the following devices: - Diluter - Probe assembly - Reaction tray - Dual beam photometer reading channel - Peristaltic pump for cuvette washer - Probe tip cleaning pump - Reaction aspiration pump - Drying pump Additional devices complete system functions include: Bar code reader Sample / Reagent tray Reagent cooler

BACK CUVETTE W ASHER BACK REACTION TRAY: 80 CUVETTES

BACK PROBE

90

ISE DISPENSING POSITION

PHOTOMETER W ITH FRONT, BACK AND REFERENCE PREAMPLIFIERS

REAGENT TRAY (INNER RING) HALOGEN LAMP

SAMPLE TRAY (OUTER RING)

FRONT REACTION TRAY: 80 CUVETTES

90

BARCODE READE

FRONT PROBE


2.2. Detail of components Sample and reagent trays Sample and reagent trays are driven by stepper motors capable of rotation in both clockwise and counter-clockwise directions. Home of tray reference is given by an optical switch, for positioning verification purposes; an additional optical sensor with slotted-wheel set, provides detection on loose of steps. Detection and recognition of samples is provided by a barcode mounted on the outer border of sample tray.

Arm assemblies front and back Arm assembly consists of two stepper motors for vertical and horizontal movements, two optical switches for sensing home position and verification for each movement working similar to sample tray. Complete assembly also includes a capacitive level detect circuitry, a probe pre-heater (for thermostatize reagent 0.5 ºC above selected reaction chamber temperature), a collision detector for prevent accidental probe damage and mixer system.

Reaction trays Reaction trays Front and Back consist of a stepper motor, optical switches home and verification sensors. Each reaction tray is loaded with 80 reaction disposable cuvettes (in strips of five units with 0.6 cm path length). Max. volume is 700uL.

Diluters Two diluters equipped with 500uL syringe each and aspirates reagent and sample consecutively. Air gaps separate liquids to prevent early mixing and contamination.

Photometer The double beam photometer is provided with 12 interference filters mounted in a rotating filter wheel, with reference channel. Light from a tungsten halogen source passes through the selected filter and a beam spitter set divides beam in three paths. One beam traverses the tray; reaction cuvette reaction tray, the other one through the cuvette for back reaction the third oneof is front directed towards a reference detector. The reading is obtained as the ratio of each signal to the reference signal, and the system is therefore immune to source fluctuations, filter variations or dirt accumulation on optical surfaces. 13 . Pictus 700 Service Manual Rev.3

This double beam design allows the detection of reaction cuvettes in the reaction tray setting an alarm when cuvettes are missing or defective.

Automatic cuvette washers There are two washing stations, one for eachreaction tray. First pipe aspirates test liquids and delivers washing solution; next three pipes deliver washing solution and empty cuvettes; and the last one contains a drying block. Operation of instrument is commanded by two intelligent microcomputers known as concentrators (C1 and C2) to handle communication packets between computer and instrument devices providing the following services: Information exchange to specific devices Parameters storage Packets tracking / detection of lost packets. Provision of a standard communication framework

Type

Attached intelligent devices • •

C1

•

• • •

• •

C2

• •

Diluters Temperature controllers Preamplifier & photometer controller. Bar code reader ISE controller

motor

Clot detector Sample, reagent and reaction trays Washers and probes. Peristaltic pumps. Spare slot functional unit

2.3. Device parameters handling Each device has internal parameters to be loaded for ensure proper operation (e.g. allowed maximum speed, required acceleration profile, configuration of sensors, internal data, internal delays, timeouts, etc). Parameter information is contained on the EEPROM memory of the concentrator and it is sent the to the device during theinstrument power-on . When computer is performing theconnect procedure to recognize the instrument, a backup of both concentrators EEPROM contentsis stored on hard disk. On the event of concentrator parameter or memory corrupted the computer will 14 . Pictus 700 Service Manual Rev.3

automatically restore the latest available backup.

2.4. Motor controller boards Except for the filter wheel motor controller, any other motor controller boards is generic and completely exchangeable, although they have different programs and parameters according the task to perform, they automatically recognize their function identifying the slot currently installed during power up. Checking any motor controller board involves just exchanging with another one.

2.5. Clot detector system The clot detector is a module to detect and warn about potential clogs or blood clots during a run. Module is composed by a pressure transducer connected between the hydraulic line of the diluter to the probe and the electronic circuit to measure, and transmits the information using the same diluter communication bus. During the instrument run, when a sample is aspirated, the vacuum in the line between diluter and probe is measured to identify abnormal pressure patterns related with clot conditions. Before a running the instrument checks the clot detector system and calibrates it. The calibration of clot detector is automatic and doesn't need any solution.

2

1


PL400306W CLOT DETECTOR BOARD

NOTE: During the first system startup of the day, it could be necessary to perform several system flushes in order to prime the clot detector.Maintenance > Opera tions > Hydraulic . tab > System Flush Software configuration:From Maintenance > Parameters > Software > Generaltab: -

Enabled check box on: behaviour on detection is possible in order to select the way instrument will handle the event of clot. Available options include, to flag the sample or invalidate it.

-

Enable check box off: Disable the clot detector feature

Installation parameter: clot detector is installed or uninstalled in Maintenance > Service > Parameters > Instrumentals > Others Debug options: From Maintenance > Service > Debug , you can mark these options: log curve when clot is detected or log curve always. If you have problems these options can help

to make a better diagnostic from factory. All information is stored in the service backup file. Important: If you mark these options you will reduce the speed of the instrument.

2.6. Electronic scale for DI water and waste bottle. It uses a load cell to weight each bottle (see picture). The weight is compared against the two thresholds to provide hysteresis. Instrument is factory calibrated with thresholds of 1/3 and 2/3 for both wash and waste bottles. When waste bottle level is more than 2/3, the waste full signal will be generated, but it will not turn off until the waste bottle volume drops below 1/3. For the water bottle level operation is similar, when available volume is less than 1/3, system will generate empty water bottle signal that will be cleared once the level is higher than 2/3. This prevents rapid switching of the signal around the set point and allows an easy interface to an external water station to the instrument. To control an external water station the instrument has in its rear panel a DB9 connector with isolated relay contact.


4

2 1 3

1- DB9 connector for external automatic water station 2- Load cell for DI water bottle. 3- Position of PL400310W load cell controller board. 17 . Pictus 700 Service Manual Rev.3

4- Load cell for waste bottle.

PL400310W load cell controller board

Setting scale thresholds The M400-P310 board has four microswitches (S1, S2, S4, and S5) to program the values of low and high level thresholds for both waste and water bottle. Those thresholds are factory pre-programmed. For both water and waste bottles, defining of the set points is similar. For example to set high level threshold for waste bottle hold on "waste high level" (S2) button for five seconds to set the high level for waste bottle. Note: The load cell controller board has 4 lights that indicated waste bottle full or empty and water bottle full or empty.

External connector specifications:


PIN OUT DB9 CONNECTOR PIN OUT DESCRIPTION

SPECIFICATION

PIN 5 – NC

-

PIN 1,6 – WASTE CONTACT 1

Voltage, Switching 24VDC MAX

PIN 2,7 – WASTE CONTACT 2

Current, Switching 100 mA

PIN 3,8 – DI WATER CONTACT 1

Current, Carry 200 mA

PIN 4,9 – DI WATER CONTACT 2

Communication between concentrators and devices:


2.7. User operation of system modules Enter to Maintenance > Operations to perform habitual maintenance tasks (e.g. purge hydraulics after wash bottle refill, reset counters after consumable parts replacement) or perform tasks requiring operation of several devices (e.g. complete dispensing cycle). Hydraulic tab: Wash . Select range and reaction tray to wash cuvettes Choose dry only when cuvettes are already clean to enhance drying. You can repeat the operation a number ofTimes . System flush. Purge both washer and tip hydraulics. Tip cleaning. Perform wash/soak of probe tip. . Operator can select volume, time of action and washing Inte nsive cuvette cleaning

solution. This action is very useful for cuvettes used with latex type of reagents or other contaminant fluids. Perform it at the end of the working day. 20 . Pictus 700 Service Manual Rev.3

. This procedure consist of using a suitable cleaning solution defined in the screen of cleaning solutions with a volume up to 500 micro liters and repeated up to 10 times (default value is 4), either back or front or both. At the end, a normal wash and drying cycle is performed. All the washer pipes are cleaned with the solution. Turn off washing pum ps. To quiet pumps after a washing operation Inte nsive w asher cleaning

Photometer tab: Press buttons to give full power or Stand by of the lamp Cuvettes. Perform a report of absorbance cuvette Motors tab: Press turns off to

de- energize all motors.

Movements tab: Perform general operations involving -sample/reagent/reaction dispensing -tip pumping -photometer readings -diluter operation -cuvette washer -move the back probe to ISE dispensing into the cup ISE tab: Startup button performs all the operations required by the module (date, wet, etc.), before starting to operate it. Cleaning operation requires to have the ISE cleaning solution in the ray. Use Empty/Fill chamber button to load/unload the ISE liquid column with the ISE cup contents. Purge button starts a process to remove the air and bubbles from the tubings. Calibration button performs the internal two-point calibration. Wet starts a sequence to maintain the membrane humidity within the appropriate levels. Dispense Std A/B activates the pinch valve to fill the ISE cup. Remember to press the fill chamber button to load the ISE liquid column. Pinch valve time button sets the dispensed volume in the ISE cup. To note, when dispensing three times Std A/B, volume must be 500uL in the ISE cup. Electrode conditioning.Perform a conditioning with serum from prime position. Versions tab: Displays firmware version and serial numbers for each device.

Wear tab: Displays amount of use cycles, limits of use and last replacement date.

2.8. Operation of manual movements 21 . Pictus 700 Service Manual Rev.3

This mode allows a quick testing of each single device to obtain additional information when user is unable to describe the specific issue. Click onData > Log as > Serv ice > type passw ord 31415 then Maintenance > service > manual to see a window having tabs to access all available modules: Vertical, Horizontal, Trays, Pumps, Washing stations, Temperature, Diluters, BCR, ISE, Photometer, Concentrator, Cool tray, Clot detector, and Stress. On the right, another two windows display the high level (command level) communications and low level (packet level) communications. Use right mouse button to open packet interpreter window and see communication details. (See section I,protocol overview).

Remember when using this mode: Set Maintenance > se rvice > debug > Manual m ovement s afety restrictions to activate the automatic protection of mechanics. Initialization of any device is always required prior to its use. Washer initialization is required before reaction tray initialization. Vertical initialization is required before sample, reagent, reaction tray or horizontal initialization. Arrow buttons on each module alow single step movement. To prevent probe crashesdo not use TURN AROUND command as it does not detect errors. For movement and device continuous test use options on stress tab and check errors.log file for results. •

• • •

• •

•

Vertical tab: Use selector to choose for front or back arms. Initialize , moves the vertical to home position. Move to position

, locates the vertical on desired number of steps down home.

Find level , moves the probe down looking for liquidlevel, slow value , is the position to start looking for level while moving slower,botto m value represents the maximum alowable steps to go down, anddown leve l value is the amount of steps to submerse the

probe into the liquid. Move up slowly button moves the boom up slowly and increases the speed after the number of steps defined by the Slow steps parameter. Turn on/off vibrator buttons. Mixer system control. Turn off motor: Unblock motor. Horizontal tab: Choose front or back arm and theninitialize to move the horizontal to home position,Move to position , to move the desired number of steps from home,Move to sample position , to 22 . Pictus 700 Service Manual Rev.3

locate an specific sample number on the tray).

into the specific sector . (Sector must be already located

Move to reage nt, reacti on, w ashing and ISE position

, work similar to previous

buttons.: Turn off motor: Unblock motor. Trays tab: Choose desired tray and useinitialize to move tray to home, Move to tray position , to advance a number of steps, and Move to sample position : for an specific sample number, sector and arm. Turn off motor: Unblock motor. Pumps tab: Select front or back, for peristaltic pump and then,initialize , advance or go back . pum p when set to 1, will be on an amount of time given by the timeout parameter. Note: set diluter to bypass position before activation of tip pump. Turn off motor: Unblock motor. Front/back Tip

Washing stations: Choose for front or back wash head, theninitialize , move to position , or move to position using a second speed for avoid spillage during drying block cleaning. Drying pump

, when set to 1 turns on the drying block.

Water suction pum Rea gent suction pum

p , when set to 1 turns on stations 2,3,4 and 5. p , when set to 1 turns on stations 1.

Turn off motor: Unblock motor. Temperature tab: Choose temperature controller thenInitialize , to tranfer internal PID parameters,read temp , set temp and disable te mp to operate temperature module.

Diluter tab: Choose front or back, Initialize And then Move to (steps, speed) Set baud rate button. Refer to Diluter replacement procedure(12.2.3). Valve positions: - bypass is pump to tip 23 . Pictus 700 Service Manual Rev.3

-

input is pump to syringe output is syringe toprobe

BCR tab: Press Initialize to start operation. Read , shows result of barcode in front of BCR device. Program , sets internal BCR

device parameters. Advanced reading options: Rea d sector code (tray position) Rea d s ample code (position)

(sect, pos) Read reagent code

ISE tab: Press Identify to check the ISE requests (date, calibration, etc.)Liquids button informs the remaining volume of standards in the kit. Use Empty/Fill chamber button to load/unload the ISE liquid column with the ISE cup contents. Purge button starts a process to remove the air and bubbles from the tubings. Send date loads the current system date into the ISE module.Urine/serum sample button sets the measurement mode. Measure triggers the measurement process. Wet button starts a sequence to maintain the membrane humidity within the appropiate levels Rinse button is used to perform a longer washing process than wetbutton. Custom button sends ISE specific commands to the module. Parameter section allows to inspect/modify parameters for the different operating modes. Photometer tab: development only screen For single reading, choose desired photometer and filter. - Start convertion and Read to show current reading. - Continuo us re ading triggers Meas. readings waiting interval mseg turn.

after each filterwheel

Concentrator tab: Press A YA button to check the communication from PC to CPU. Press Reset button to perform a reset of complete instrument. Cool tray tab: Press Read temp. Set temp erature

to display current temperature (it shows zero when cool tray is off). will set both high and low trips. 24


PRESS INSTRUMENT GREEN PUSHBUTTON TO TURNON THE COOL TRAY. Clot detector tab: Press Get version button to know the firmware version and check the communication to clot detector board.. Press Calibrate

button to perform a simulation of clot detector calibration.

Stress tab: It moves randomly desired number of cycles and show errors of each movement. Check errorslog.txt to see errors details. Warning: Do not try to stress both horizontal and vertical at the same time as further checking is performed.

no


3.

Troubleshooting

3.1. System tests System tests are designed in order to detect any abnormality in the equipment operation. In order access to the system test window, you should first log as serviceData ( > Log as > Service > passwo rd 31415 ) then go to Maintenance > System Test. Results of tests are stored in PDF files in the Working Directory\System Test folder .

3.1.1.

Temperature

This test measures the time required for reaching preset temperatures and final stability of reading. It includes cooler tray temperature. The test records the minimum reached temperature and the last time in which the temperature reached the band between minimum and maximum allowed values. If the temperature is turned off, the test stops and a warning is issued.

3.1.2.

Stray light

This test is based on the use of two solutions: one uv sharp blocking and other visible blocking. Visible blocking is usually Potassium Chromate in high concentration (more than 5 g/l) and should block all light passing the cuvette at the specified wavelength. If it fails, light is arriving directly to sensor without traveling through the cuvette. UV blocking, if visible is passed, indicates actual filter stray light. The use of Sodium Nitrite, 50 g/l and reading at 340 nm is recommended. There are two options: either instrument dispenses solutions or user put them directly in the selected cuvettes. Use “Already dispensed” for selection. Test is passed if read values are less than 0.1%T. Volume selection can be used to determine minimum volume that can be safely measured.

3.1.3.

Noise

This test determines the departure of individual readings from the mean value. Noise is evaluated separately from drift. For stability evaluation, total time (Number of readings X Time interval) should be at least 10 minutes. Noise evaluation is performed without moving tray and data are directly related to photometer behavior. When Absorbance correction is selected (recommended for solutions and not for filters), results are expressed as equivalent to 1 cm cuvette measurements. Noise test is relevant for absorbances over 1.300. Potassium Chromate (1.2 to 1.5 g/l in acidic media) is recommended. Relevant data are peak-to-peak (maximum) difference. They should not exceed 0.002 for 1 minute total time.


3.1.4.

Stability

Stability test is very similar to noise test, but the tray is randomly moving between readings. Comparison of data from noise and stability tests can give a hint on mechanical positioning problems. Use conditions as described in3.1.3

3.1.5.

Tip Pump

This test allows determining if washing tip pump is delivering the correct amount of water. Procedure is performed in a reagent bottle located in position 1, where initial liquid level should be at least to a height of 2 cm. Procedure is repeated several times and averaged.

3.1.6.

Level detection

Reagent is taken from a vial located in a fixed position and tip reaches the surface of other reagent located in a different position. Next, reagent is delivered in the srcinal one. This procedure is sequentially repeated while volume is varied every cycle within fixed limits. Results and plot will show if level detection is accurate. Use this test if detection problems are observed with a given method or Brand. When using, be sure that no foam is present in reagents or samples. If test is repeated many times, be sure that no foam is formed in the process.

3.1.7.

Washer hydraulics

Calibration for cuvette bottom should be performed, prior to other operations. A liquid pumping level check will be performed by dispensing water with D1 to D4 and measuring the liquid level with sensor probe (positions 2 to 5). The calculated volume will be recorded for each station (1 to 4). A liquid suction level check will be performed drawing water with S1 to S5 and measuring the liquid level (if any) with sensor probe. To be able to measure such small remnant amount, the syringe full loaded with water (500µL) will dispense 100 µL in each position (1 to 5). The calculated remnant volume will be recorded for each station (1 to 5). A liquid pumping level stability test will be performed dispensing water with D1 to D4, the number of times determined by the parameter, in different cuvettes (positions 6 to 9, 10 to 13,) and measuring the liquid level with sensor probe. The following volumes will be recorded for each station (1 to 4): individual measurements; average volume; standard deviation and variance; minimum and maximum volumes; difference between minimum and maximum volumes; difference between minimum and maximum average volumes of the 4 stations; relative deviation error of average volumes. A cuvette wash will be performed in all the used cuvettes.


3.1.8.

Washer

Washer test consists of performing cuvette cleaning cycle on a programmed number of cuvettes. Absorbances are read on new cuvettes before cleaning action, immediately after cleaning and at some fixed time (Drying time). All three data are shown in the graph. If cuvettes are properly dried and not scratched by the system, values should return to the srcinal ones, with a tolerance of about 0.020 abs.

3.1.9.

Dilution

Dilution test should be performed with a sample of Potassium Chromate of 5 g/l in acidic solution. Use as reagent the tip washing solution. For a final volume of 4/400, CV should be less than 1.5%

3.1.10. Photometer linearity This test is intended for evaluation of photometer linearity. To achieve this goal, the test will measure absorbance (A0 to A4) of 5 different solutions (points 0 to 4) in front and back channels using a specific filter. Each solution will be prepared by dilution of a stock solution located in a specific on-tray position. For a default initial sample volume of 8 microliters, system will automatically generate dilutions of 0/300, 8/292, 16/284, etc. maintaining the total solution volume unchanged. A cuvette diluent blank will be performed prior to dispensing using a certain volume. Dilution will use the remaining volume of diluent. After dispensing, a certain time will be observed prior to reading. A certain number of replicates will be done for each point. This test differs from 3.1.11 in the range of volumes. For volumes above 8 microliters, it is assumed that diluter linearity is out of question and any linearity departure is related to electronics or optics.

3.1.11. Diluter linearity This test requires a concentrated Potassium Chromate solution (3 g/l in Perchloric 5 mmol/l) and washing solution as reagents. Given an initial volume (3µl as default) system will generate dilutions using 1, 2, 3, 4 times the initial volume. Linear correlation and departure from linearity are evaluated. Departures of +/- 5% are accepted.

3.1.12. Chemistry analysis Test allows selecting any method from any already defined Control Set and perform statistical analysis. Several analysis on methods belonging to the same control can be measured. Precisions are set default in 3% but operator should decide the required level.

3.1.13. Clot detector Several determinations can be made over both detectors (front and back): 1. Noise (dispersion): perform an evaluation of the pressure sensor baseline, this is to

identify any issue from electronics srcin or power supply related issue. 2. Calibration: Determines a Factor (CF) is asimilar routine to the calibration at the beginning of a run. 28 . Pictus 700 Service Manual Rev.3

3. Dilution Test can be performed with a sample or with water and a real reagent in tray. If the real used sample has high density or clot the test will fail, but this apparent failure is the verification of proper detector functioning, if the calibration is successful.

3.2. Visible faults General faults Symptom

Corrective Action

Drops on probe tip after dispensing

Verify hydraulic system in accordance to user’s manual. Clean probe tip by submerging in Solution 1 for 5 minutes. Drops on tip after wash cycle. Verify hydraulic system for leaks or obstructions. Abnormal noises. Defective fans. Moving parts blocked or frozen. Temperature in reaction tray is too high. (Do Room temperature too high, (should always not be concerned about arm probe be at least 4°C lower than selected working temperature) temperature). Example: For 37°C incubation temperature, Room temperature should not exceed 33°C. Temperature in reaction tray is too low. (DoRoom temperature excessively low. Verify not be concerned about arm probe instrument operating range, and adequate temperature) the room temperature.

3.3. Measurement inconsistencies Consider storage and handling of reagents, standards and controls: 1. Verify expiration date, storagetemperatures on and off analyzer. Check that reagent was not frozen. 2. Check color changes, sediments, turbidity, no foam. 3. Check not for mixed reagents from different lots nor re-use of reagent ottles. b All methods 1. Verify cuvettes for dirt or scratching. 2. 3. 4. 5.

Remove cuvettes from reaction tray andcheck volumes for affected cuvettes. Verify there are no bubbles or droplet. Verify there are no obstructions on probe, check for non constant orregular flow. Recalibrate photometer.

6. Perform energy, noise andphotometric stability tests.


Colorimetrics with high dispersion 1. Replace sample by a standard and verify dispersion again, check reaction cuvettebeam alignment. 2. Perform hydraulic verification. 3. Check for sample centrifugation, increase time and speed. 4. Perform energy, noise photometric stability and dilution tests.

Colorimetrics with proper dispersion but values too high or low 1. Verify standard, compare calculated factor with stored (historic) factors, and recalibrate method. If problem persists, replace standard and/or reagent. 2. Clean probe and check for cross contamination by changing the order of dispensing (“Time priority for reagents” parameter). Check proper probe washing/cleanprobe. 3. Check for exceeded methodlinear range; compare method definition with reagent specification. 4. Verify sample volume is not excessive. 5. Perform stray light verification, high range andlow range linearity test, and dilution test.

Kinetics with high dispersion or low linearity 1. Verify if incubation time is too short or heaters are not working properly. 2. Verify for abnormally high initial absorbance for decreasing kinetics (problems with reagent preparation) or too low on increasing kinetics. Replace reagents and compare results. 3. Check lamp for stability. 4. Use new cuvettes and test again, check cuvettes for dirt or scratching. 5. Perform noise and photometric stability, align lamp, clean filters, and check reaction tray-beam alignment. 6. For some kinetics: verify if sample volume is too low. 7. For some kinetics: verify centrifugation (increase time andspeed).

Kinetics with normal values too high 1. 2. 3. 4.

Perform energy, noise and photometric stability. Perform temperature verification. Some kinetics: incorrect factor for selected temperature andvolume. Replace lamp, align lamp, clean filters verify reaction tray positioning.

Kinetics with normal and pathological values too high 1. Verify incubation time and temperature. Perform temperature test. 2. Verify if factor matches selected temperature. Remember selected temperature is usually 37 oC 30 . Pictus 700 Service Manual Rev.3

Kinetics with normal and pathological values too low 1. Check for short incubation time or lowtemperature. 2. Verify if factor matches selected temperature. Remember selected temperature is usually 37 oC.

Kinetics with values too low or too high on the whole range Verify if factor matches selected temperature. Remember selected temperature is o

usually 37 C. Two point kinetics (high dispersion) 1. Verify if standard absorbance is too low (verify data provided by standard manufacturer). 2. Verify initial consumption is toohigh (verify data provided reagentmanufacturer). 3. Perform energy tests. 4. Verify reagent handling andstorage. 5. Check method parameter for reagent, low sample volume or too short interval times. 6. Check time table for dispersion onfirst measurement.

Two point kinetics (high dispersion) Verify factor, standard and method for reagent. Repetition or dilution (colorimetrics or non linear kinetics) 1. Verify if sample volume is too high, check reagent linear limit. 2. Replace reagent and compare. Repetition or dilution (two pointkinetics) This is not actually an error; it is because volume / absorbance change relationship is not linear, and so it is necessary to dilute standard and compare. Reactions (general comparison between reactions) 1. 2. 3. 4. 5.

Control quality of water. Verify proper usage ofsolutions (wash solution, rinse solution, etc). Use uric acid to check water quality. Verify for frosted cuvettes (presence ofsalts). Verify scratching or old reactions residues (not enoughwashing).

6. Perform instrument validation tests.


Clot detector troubleshooting

Symptom

Corrective Action

–

–

Clot detector system calibration error, before a run. – False positive clot detection. – Fail system test -> Calibration

–

Fail system test -> Dilution

–

Fail system test -> Noise

Verify hydraulic system for leaks or obstructions. (Tips, tubes, connectors) – Purge hydraulic system. Perform several system flushes. – Verify air bubbles in the hidraulic system Clot detector board – – Verify hydraulic system for leaks or obstructions. (Tips, tubes, connectors) – Purge hydraulic system. Perform several system flushes. – Verify air bubbles in the hidraulic system – Verify use DI water for reagent and sample for this test, – Clot detector board, Clot detector board. – – Power supply of clot detector board. – Power supply cables

Electronic scale troubleshooting

Symptom

Corrective Action

–

– Bad calibration of scale thresholds in the controller board setting again.

Don't change filling status of bottles.

–

–

Don't repeat threshold levels.

Check base of bottles, it should be move free and only touch load cell. – Connector of load cell is not connected – Load cell is connected in a wrong position. Bad calibration of scale thresholds in – the controller board setting again.

–

Check base of bottles, it should be

move free and only touch load cell.


3.4. Verification and diagnostic procedures 3.4.1.

Standard troubleshooting procedure

1. Identify symptom Obtain a detailed description about the issue from a skilled instrument end user (avoid unspecific descriptions). Ask for any symptom (leaks, noises, beeps, last operations performed before the event) Review section II,general troubleshootingto identify additional actions to be performed by user to obtain extra information. •

•

•

2. Recognize variables affecting the result Group togheter symptom or problems to isolate the main cause, consider settings of affected methods (e.g. results are lower than expected on high controls, unstability on methods having low sample volume). •

3. Use diagnostic tools to isolate the main cause (do not perform resolution steps before identifying main causes): Manual movements window Instrument tests Information provided on errors.log file. • • •

4. Fix the cause Run fresh controls Remove jams, tighten connections Repair or replace • • •

5. Check that solution worked Use same tools used before to check resolution of issue. •

3.4.2.

Reload instrument parameters being offline

In order to check the memory backup of another instrument, you can read the information from the MemoryBackup.ini file into the software. 1. Log as service (Data > Log as > Service > passw ord 31415) 2. From Data > load param eters select the memorybackup.ini you want to load.


3.4.3.

General Voltage checking WARNING:

Reagent cooler power supply is turned on by the greenpushbutton.

1. Turn on instrument. 2. Check if red power push button is blinking (No blink=all power supplies areOK). 3. Check the green led located on each power supply.4. In case led is off, test the power supply without load, follow schematic diagrams to find the short circuit. 4. Disable power on off board (see schematics).


3.4.4.

Instrument general status check

Choose Maintenance > E lectronic status , instrument will show its internal status as shown below: Board status FRTW Temperature: 36ºC Voltage: 37 BRTW Temperature: 32 ºC Voltage: 37 SRT Temperature: 43 ºC Voltage: 36 FBPP Temperature:35 32ººC Voltage:37 37 FVH Temperature: C Voltage: BVH Temperature: 33 ºC Voltage: 36 System boards status Id: 0 Ok Id: 1 Ok Id: 2 Ok Id: 3 Ok Id: 4 Ok Id: 5 Ok Id: 6 Ok Id: 7 Ok Id: 8 Ok Id: 9 Ok Id: 10 Ok Id: 11 Ok Id: 12 Ok Id: 13 Ok Cooler thermostat: Ok

3.4.5. Tray &

Movement test Note: This kind of test will move randomly the selected device with no further checking. Do not check two conflictive devices simultaneously (e.g. do not test both vertical and horizontal at the same time).

1. Enter to Data > Log as > Service > passwo rd 31415, then enter to Maintenance > Service > Manual select stress tab. 2. Select movement or operation to test. 3. Set movements to 100, press white hand button to start 4. Review results on errors.log file When complete tray and arm movement test is required, useMaintena nce > sys tem test > Level dete ctio n.


3.4.6.

Belt tension measurement and value settings

To measure the belt tension. We recommend using the follow instrument:

SONIC TENSION METER MODEL:507C BRAND:GATES For more information please refer to user manual of sonic tension meter.

Tension values and parameters for tension meter. MOVEMENTS

VERTICAL

HORIZONTAL

SN: SN: PARAMETERS XXXX41XX XXXX40XX Cat. Nº 147 MXL 136 MXL Mass 1,3 1,3 With 6 6 Span 170 150 Freq. Range Standard Standard Value 13 -17 13 -17

Cat. Nº Mass With Span

2MR-186-06

1,3 6 50

72MXL

1,3 6 50 36


REACTION

SAMPLE

REAGENT

WASHER

Freq. Range Standard Standard Value 25-40 25-40 Cat. Nº 3MR- 630-06 280XL Mass 4,5 2,4 With 6 6 Span 205 180 Freq. Range Standard Standard Value 27-60 27-60 Cat. Nº 283XL 283XL Mass With Span Freq. Range Value Cat. Nº 220XL Mass With Span Freq. Range Value Cat. Nº Mass With

2,4 10 70 Standard 18-22 220XL 2,4 10 120 Standard 30-35 640 MXL 1,3 6

2,4 10 70 Standard 18-22

Span Freq. Range Value

60 Standard 18-22

60 Standard 18-22

2,4 10 120 Standard 27-60 640 MXL 1,3 6

3.5. Protocol Overview Communication is first established when the computer sends a request packet containing a command to the specified device attached to the specified concentrator. After checking the valid device address, concentrator will answer with an acknowledge packet and will forward a short packet to the device containing both commands and required parameters. When command is completed, device will produce a response packet to the concentrator which will be translated into an answer packet to the computer. Once the computer receives the packet it will produce another acknowledge packet for the concentrator. 1. REQUEST PC

2. ACK to REQ 3. ANSWER

C1 OR C2

4. ACK to ANS


Operation data flow

3.5.1.

Packet tracking

Detection of missing packets is achieved using packet counter for computer (PC_PKT#)m for C1 (C1_PKT#) and C2 (C2_PKT#). - When the computer sends a request, the acknowledge packet will contain the same packet number provided by the computer. - The answer packet will contain the packet number of the specific concentrator and the further acknowledge from the computer will repeat the same packet number.

1. REQUEST PKT# = PC_PKT# 2. ACKPKT# = PC_PKT#

PC 3. ANSW ERPKT# = CX_PKT#

C1 OR C2

4. ACK PKT# = CX_PKT#

Rule for packet counting

3.5.2.

C1 Protocol

Concentrator 1 includes one RS-232 port specifically for the bar code reader, a RS-485 port for the preamplifiers, and another RS-485 port sharing a common protocol for thetemperature controllers, diluters, photometer motor controller and the protocol translator unit for the ISE module.


3.5.3.

C2 Protocol

Concentrator 2 uses a TTL interfase to handle all the movement-related devices. After the request transmited by the C2, an acknowledge packet is returned by the device, then C2 will query the device at regular times until obtain a valid answer.

Re uest ACK

2nd Concentrator

Quer

FU

Query

Query Answer

3.5.4.

Protocol layers

When computer to instrument communication is analyzed two different bit streams are provided for understanding the operation. The high level comm unicat ion only contains the commands, parameters, and answers from the device, useful for the service diagnostic (see section I, understanding errors.log). The low level comm unicat ion includes all kind of packets transmited between the instrument and the computer, including requests packets, acknowledge to request packets, answer packets, and acknowledge to answer packets. Also each packet complete contents is shown including packet numbers and crc values. A detailed description on low level communication is out of the scope of this manual


3.6. Understanding errors.log When a single error event is produced, information is automatically stored on errors.log file (no matter if instrument recovered from event and finished processing). Dump of errors.log file is composed by several parts: Error code + Error Message

[E.0206.S.03] Sample Tray - Home sensor - 09/06/2006 01:36:18 p.m.

Software development tracing information

THabInicializarTodas-THabInicUnidFunc-TAcPlatoInic

High level communication (single command)

High level comm Tx: 0206 00 Rx: 0206 00000000 00 00030000

Coordinates after sucessful command

Front dilutor :0 Back diluter :0 Front horizontal : 139 Back horizontal : 140 Front washer :0 Back washer :0 Sample tray :0 Reagent tray :0 Front reaction tray : 0 Back reaction tray : 0 Front vertical : 131 Back vertical : 120

Low

level

the

last

communication

(including numbers ACK packets information).

Low level 621 622 623 624 625

comm Tx: 06000208007C006026 Rx: 05010208007C15BC Tx: 06000209007D000C26 Rx: 05010209007D152C Rx: 0E0002080078000000000000000000E7AB

13:36:9.812 13:36:9.828 13:36:9.828 13:36:9.828 13:36:10.125

packets, and CRC

On next page, a table indicates how to translate high level communication to obtain device specific details.


High level protocol Request

Respond

CX +FU

01

03E8

OpCode

Parameters

0206 address

Device

Addr ( CX+ FU)

C1 C2

01FF 02FF

C1 & C2 (broadcast)

FFFF

C2/Sample tray C2/Reagent tray C2/Reaction Front tray C2/Reaction Back tray

0206 0207 0202 0204

C1/PhotometerF C1/PhotometerB C1/Photometer Ref

C2/Vertical F C2/Vertical B

0101 0102 0103

0210 0212

Front

Request OpCode 00: memory read 1byte 01: memory read 2bytes 02: memory write 1byte 03: memory write 2bytes 05: ping 06: reset 07: memory read 64bytes 08: memory write 32bytes 09: read fridge temp (c2 only) 0A: set fridge tmp trip points (C2 only) 0B: status request 0C: read fridge tmp trips (c2 only) 0D: clear int params not loaded bit 0E: clear CRC flash bit 06: reset 00: init 01: move 04: turn around 0A: set output 0B: clear output 0D: read input 10: temp read (FRT, BRT & ST) 11: voltage read (FRT, BRT & ST) 00: start convertion 01: read 02: read bichr. 03: stop conversion 04: raw read 05: start communication test 06: start channel measurement test 07: start zero measurement test 08: raw read 09: read dual 0a: start variable readings 0b: read variable one channel readings 0c: turn to still mode 0d: return to normal mode 00: init 01: move 04: turn around 0C: down to level 13: down to level (slow) 0A: set output 0B: clear output 0D: read input 0F: move 10: read temp 11: read voltage

status**

Device Status bits (active on error)

Param Address (2b) Address (2b) Address(2b)+value(1b) Address(2b)+value(1b) - Address(2b) Address(2b)+value(32b) 1b Hightrip+ 1b Lowtrip position (2b) # of turns (2b) 1 to set bit (2b) **** 1 to clear bit (2b) **** - smple(4b)+smple.0(4b)+ref(4b)+ref.0(4b) same as single read w/two diff wl (32b total) F or B(80b)+ref(80b)+ smple.0(4 b)+ref.0(4b) - smple.0(80b)+ref.0(80b) F(80b)+B(80b)+F.0(80b)+B.0(80b) channel(4nb)+channel.0(4b) -

position (2b) # of turns (2b) bottom( 2b)+downs tep(1b) bott(2b)+d wn(1b)+slo wstep(1b) 1 to set bit (2b) **** 1 to clear bit (2b) **** position( 2b)+lowspe edsteps(2b ) -

0=Write error 0=Write error 0=Bad memory (on C1)

0=Write error 0=Read error 0=Error

0=Home 1=Verif 4=Temp 5=DigPot B6=Voltage 0=Home 1=Verif 2=Not init 3=Wrong pos 4=Temp 6=Voltage 4=Temp 6=Voltage 7=Collision

0=Error 0=Error 7=No data from preamps. 0=Error 7=No data from preamps.

0=Home 4=Temp 5=DigPot 6=Voltage 7=Collision 0=Home 1=Verif 2=Not init 3=Wrong pos 4=Temp 6=Voltage 7=Collision 4=Temp 6=Voltage 7=Collision 1=Verif 2=Not init 3=Wrong pos 4=Temp 5=No level 6=Voltage 7=Collision 0=Early level 1=Verif 2=Not init 3=Wrong pos 4=Temp 5=No level 6=Voltage 7=Collision -

Respond Value (1b) Value (2b) Values (64b) Signed float (4b) see notes below *** Signed float: Hightrip + Lowtrip Prev position (2b) Curr position (2b) (2b) **** temp (1b) voltage (1b) Smpl(2b)+Smpl.0 (2b)+ref(2b)+R ef.0(2b) Same of above repeated twice

Prev position(2b) Curr position(2b) Currposition(2b) Curr position(2b) (2b) **** - Temp(1b) Voltage(1b)


Device

C2/Horizontal F C2/Horizontal B

Addr ( CX+ FU)

0211 0213

C2/Pump F C2/Pump B

0208 0209

C1/Diluter F C1/Diluter B

0110 0111

C2/Cuv washer F C2/Cuv washer B

0203 0205

C1/React. temp. Cont C1/F probe temp. Cont C1/B probe temp. Cont

0114 0115 0116

Request OpCode 00: init 01: move 04: turn around 0A: set output 0B: clear output 0D: readinput 12: shake 00: init 06: forward probe pump 07: backward probe pump 08: forward washer pump 09: backward washer pump 0A: set output 0B: clear output 0D: read input 10: read temp(F Only) 11: read voltage (F Only) 00: init 01: move 02: bypass or throughput position 03: output (right) position 04: input (left)position 00: init 01: move 04: turn around 0A: set output 0B: clear output 0D: readinput 00: init 01: read temp 02: set temp 03: disable temp

C1/Photom motor Cont

0118

00: process parameter as a command

C1/ISE

01AA

C1/BCR

0120

N/A 00: init 01: read 02: program

Param position (2b) # of turns (2b) 1 to set bit (2b) **** 1 to clear bit (2b)**** #oscil(2b)+amp(1b)+slope(2b) steps(2b) steps(2b) steps(2b) steps(2b) 1 to set bit (2b)**** 1 to clear bit(2b) **** position(2b)+speed(2b) position(2b) #turns(2b) 1 to set bit (2b) **** 1 to clear bit(2b) **** temp(1b) Start: 41 Stop: 4F Position: “66” + filter#(2b) + offset(1b) Speed: “56” + value (5b) Slope: “4C” + value (2b) Pulses per turn: “50” + value (3b) #filters: “46” + value (2b) HomeToZeroSteps:“6F” + value(2b) QuerySWVersion: 3F52 QueryControllerId: 3F49 QueryTemp: 3F54 QueryVoltg:3F6D QueryChopperCurrent: 3F63 QueryHometoZerofilter:3F6F All values given in ASCII N/A string (3b)

Device Status bits (active on error) 0=Home 1=Verif 4=Temp 5=DigPot B6=Voltage 0=Home 1=Verif 2=Notinit 3=Wrong pos 4=Temp 6=Voltage 4=Temp 6=Voltage 0=Home 1=Verif 2=Notinit 3=Wrong pos 4=Temp 6=Voltage X=Humidity 4=Temp 5=DigPot 6=Voltage X=Humidity 4=Temp 6=Voltage X=Humidity4=Temp 6=Voltage X=Humidity4=Temp 6=Voltage X=Humidity4=Temp 6=Voltage X=Humidity4=Temp 6=Voltage X=Humidity4=Temp 6=Voltage X=Humidity4=Temp 6=Voltage X=Humidity4=Temp 6=Voltage X=Humidity4=Temp 6=Voltage 0=Syr.Jammed 1=ValveJammed2=intern param 3=invalid syr.mov 4=init 0=Syr.Jammed 1=ValveJammed2=intern param 3=invalid syr.mov 4=init 0=Syr.Jammed 1=ValveJammed2=intern param 3=invalid syr.mov 4=init 0=Syr.Jammed 1=ValveJammed2=intern param 3=invalid syr.mov 4=init 0=Syr.Jammed 1=ValveJammed2=intern param 3=invalid syr.mov 4=init 0=Home 4=Temp5=DigPot 6=Voltage 0=Home 2=Not init 3=Wrong pos 4=Temp 6=Voltage 7=Collision 4=Temp 6=Voltage 7=Collision 0=Invalid comm 1=out of range 2=Transmision 3=Q err 4=bufferfull 0=Invalid comm 1=out of range 2=Transmision 3=Q err 4=buffer full 0=Invalid comm 1=out of range 2=Transmision 3=Q err 4=bufferfull 0=Invalid comm 1=out of range 2=Transmision 3=Q err 4=buffer full Same status values for every command: 60: No error 61: Filter wheel not initialized 62: Invalid command 63: Invalid parameter 64: step loss, invalid home detection 65: locked wheel, home sensor not found 66: invalid command 67: Temp higher than 58C 68: Temp higher than63C 69: Voltage out of range (lower than 32Vor higher than40V) 6A: Chopper voltage out of range (valid range: 460mA+-20%)

N/A N/A N/A N/A

Respond Last position (2b) Current position (2b) (2b) **** Current position (2b) Prev position (2b) (2b) **** (1b) (1b) Prev position (2b) Curr position (2b) (2b) **** value(2b) -

(4b) (16b) (2b) (2b) (4b) (2b)

N/A code(20b max) or NO READ/NO ANSWER string (3b)


Notes * Req Code

7 -

6 -

5

4

3

2

1

0

CX-Device No answer

Invalid OpCode

Invalid parameters

Invalid Address

Device Busy

Device not init.

** C1 System Status on respond

Vertical &

7…0 Not in use

7…0 Not in use

Horizontal

** C2 System Status on respond 7…0 Not in use

Back

***Answer to status request opcode (0B): C1: Byte 0, bit 0= ISE ERROR; byte 1, bit 0= Ise interfase error; byte 2, bit0= BCR error; byte 3, bit0= Filter wheel error; Byte 4, bit 0= Front preamp; byte 5, bit 0= Back preamp; byte 6, bit 0= Ref preamp; byte 7, bit 0= Front temp; byte 8, bit 0= Back temp; byte 9, bit 0= React temp; byte 10, bit 0=Front, bit 1=Power supply err; byte 11, bit 0=Back dil C2: -

Bytes 0 to 13 represents FU #0-13 given: Bit 0- Power supply voltage, 1- Chopper voltage, 2- Temp, 7- Comm Byte 14 represent cooler controller: 0- Comm, 1- High trip and Low trip check Byte 15 check concentrador -0Duart Nº1 CHA, -1 Duart Nº1 CHB

**** Digital inputs/outputs Addr 0202 0204 0206 0207 0211 0213 0210 0212 0208 0209 0203 0205

Type O I O I O I O I O I O I O I O I O I O I O I O I

Bit detail 8=Led0 9=Led1 10=Led2 11=MotorOn 0=Home1=Verif 4=Humidity 0=Lamp 8=Led0 9=Led1 10=Led2 11=MotorOn 0=Home1=Verif 4=Humidity 8=Led0 9=Led1 10=Led2 11=MotorOn 0=Home1=Verif 4=Humidity 8=Led0 9=Led1 10=Led2 11=MotorOn 0=Home1=Verif 4=Humidity 8=Led0 9=Led1 10=Led2 11=MotorOn 0=Home1=Verif 3=Collision 4=Humidity 8=Led0 9=Led1 10=Led2 11=MotorOn 0=Home1=Verif 3=Collision 4=Humidity 1=Mixer 8=Led0 9=Led1 10=Led2 11=MotorOn 0=Home1=Verif 2=Level 3=Collision 4 =Humidity 1=Mixer 8=Led0 9=Led1 10=Led2 11=MotorOn 0=Home1=Verif 2=Level 3=Collision 4=Humidity 0=TipPump 8=Led0 9=Led1 10=Led2 11=MotorOn 0=Home1=Verif 2=Level 3=Collision 4=Humidity 0=TipPump 8=Led0 9=Led1 10=Led2 11=MotorOn 0=Home1=Verif 2=Level 3=Collision 4=Humidity 0=Station1 1=Dryier 2=St2, St3, St4 8=Led0 9=Led1 10=Led2 11=MotorOn 0=Home1=Verif 2=Level 3=Collision 4 =Humidity 0=Station1 1=Dryier 2=St2, St3, St48=Led0 9=Led1 10=Led2 11=MotorOn 0=Home1=Verif 2=Level 3=Collision 4=Humidity


4.

Mechanical Calibrations

In order access to the mechanical calibration window, you should first log as serviceData ( > Log as > Service > password 31415 ) then go to Maintenance > Calibration > Mechanical.

All the mechanical calibration parameters are stored in the CPU memory.

4.1. Photometer This calibration will determine the optimum reading position in the middle of each cuvette. 1. Select Front Tray, remove cuvette cover. 2. Press F1 function or Start button 3. Use buttons or letters Q and E in keyboarduntil cuvette number 3 is close to photometer position. Use 10 steps or 1 step option as required. Photometer position is labeled with an arrow. 4. Close cover 5. Press Scan button. Instrument will scan cuvette number 3 and in Position window will write optimum calibration value. 6. Press F3 function or Confirm button. 7. Select Back Tray and repeat procedure. If instrument was already calibrated,Last button will position tray where last calibration was determined. This procedure will save time and item 3 can be skipped. Once Start button is pressed, calibration can be aborted by pressing theSkip button.

4.2. Arm and Reaction Tray This calibration will define that tip falls in the reaction tray in the middle of the reaction cuvette. Also, it defines the cuvette vertical position, which in turn, will define the dispensing height. Calibration includes positioning ofcuvette washer module. 1. Select Front Tray; remove cuvette cover and cuvette retainer cover. 2. Press F1 function or Start button. 3. Use buttons or letters Aand D in keyboard until tip is close to the center of cuvettes. Use 10-step or 1-step option as required. 4. Use buttons or letters W and S in keyboard until tip is few millimeters above cuvette. 5. Rotate tray by using buttons or letters Q and E in keyboard until cuvette number 1 (labeled with a sticker) coincides with tip position. 44 . Pictus 700 Service Manual Rev.3

6. Repeat steps 3 and 5 until tip falls in the middle of cuvette number 1. For better sensitivity, use 1-step buttons. Do not fine tune vertical position at this time. . Press F3 function or Confirm button 8. Press F3 function orConfirm button. 9. Shift probe horizontally until tip is above cuvette body but outside cuvette itself. 10. Use buttons or letters W and S in keyboard and 1step mode until tip just touches upper flat part of cuvette body. 11. Press F3 function orConfirm button. 12. Select Back Tray and repeat procedure.

If instrument was already calibrated,Last button will position tray where last calibration was determined. This procedure will save time and item 3 can be skipped. Once Start button is pressed, partial calibrations can be aborted by pressing the Skip button. Important: Before to confirm a position, press F5 to test this movement in normal operation to avoid a bad calibration due to backlash. Note: Last button does not act on vertical positions. This is so to prevent tip damage.

4.3. Washer Use this window to calibrate the position when the drying block goes down to a cuvette. From the washer calibration window 1. Choose front or back washers, and then push Start. 2. Loosen washer head screws.


3. Use buttons or letters R and F in keyboard to download the washer into the cuvette. When moving down the washer, use the index finger to move up the dryer block, release it, and check if moves down freely. Center washer pipes in the cuvette. Pre adjust screws.

4. Continue moving down until the dryer block reaches the cuvette bottom. The dryer spring should not be activated. Stop before it happens.

5.

Confirm than the dryer block moves freely, and the pipes are centered in the cuvette and tighten screws.


6.

Continue moving down until station 5 spring compresses about 0.5 mm. The block dryer block should not move the reaction tray, you have to test it with test button. Use 10-step or 1-step option as required.

7. Push Skip to cancel or Confirm to accept the calibration. 8. From the Maintenance >Operation window, wash 10 cuvettes 5 times. 9. Check if the washer is not scratching the cuvettes.

4.4. Arm and Washing Station 1. Select Front Tray. 2. Press F1 function or Start button. 3. Position match from left movement. Use buttons or letters A and D in keyboard until tip is close to the center of washing station and touch the bottom. Use 10-step or 1-step option as required. 4. Press F3 function or Confirm button. 5. Position match from right movement. Use buttons or letters A and D in keyboard until tip is close to the center of washing station and touch the bottom. Use 10-step or 1-step option as required. 6. Press F3 function or Confirm

button.

Important: Before to confirm a position, press F5 to test this movement in normal operation to avoid a bad calibration due to backlash. 47 . Pictus 700 Service Manual Rev.3

4.5. Arm and Sample 1. Select Front Probe. 2. Press F1 function or Start button. 3. Use buttons or letters Aand D in keyboard until tip is close to the centerofinner sample ring. Use 10-step or 1-step option as required. 4. Rotate Sample tray by using buttons or keys Q and E in keyboard. 5. Repeat 3 and 4 until tip is in the center of samplevial number 1. 6. Press F3 function or Confirm button. 7. Use buttons or letters W and S until tip just touches bottomof sample vial. Pull up frequently the vial while stepping down 8. Press F3 function or Confirm button. 9. Use buttons or letters A and D in keyboard until tip is close to the center ofouter sample ring. Use 10-step or 1-step option as required. 10. Rotate Sample tray by using buttons or keys Q and E in keyboard. 11. Repeat 3 and 4 until tip is in the center of sample vial number 2. 12. Press F3 function orConfirm button. 13. Use buttons or letters W and S until tip just touches bottom of sample vial. Pull up frequently the vial while stepping down 14. Press F3 function orConfirm button. 15. Select Back Probe and repeat procedure. If instrument was already calibrated,Last button will position tray where last calibration was determined. This procedure will save time and items 3, 4, 9, and 10 can be skipped. Once Start button is pressed, partial calibrations can be aborted by pressing the Skip button. Important: Before to confirm a position, press F5 to test this movement in normal operation to avoid a bad calibration due to backlash.

Note: Last button does not act on vertical positions. This is so to prevent tip damage


4.6. Arm and Reagent Tray 1. 2. 3. 4.

Fully remove the reagent cover. Select Front Probe. Press F1 function or Start button. Use buttons or letters Aand D in keyboard until tip is close to the center of cap of outer reagent ring. Use 10-step or 1-step option as required. 5. Rotate Reagent tray by using buttons or keys Q and E in keyboard. 6. Repeat 3 and 4 until tip is in the center of cap of reagentvial number 1. 7. Use buttons or letters W and S until tip just touches cap of reagent vial. 8. Press F3 function or Confirm button. 9. Use buttons or letters Aand D in keyboard until tip is close to the centerofinner reagent ring. Use 10-step or 1-step option as required. 10. Rotate Reagent tray by using buttons or keys Q and E in keyboard. 11. Repeat 3 and 4 until tip is in the center of reagentvial number 25 and vial 49 (physically located as the inner split vial on position 25 ).. 12. Use buttons or letters W and S until tip just touches cap of reagent vial. 13. Press F3 function orConfirm button. 14. Use buttons to achieve bottom of reagent 1. 15. Press F3 function orConfirm button. 16. Select Back Probe and repeat procedure.

If instrument was already calibrated,Last button will position tray where last calibration was determined. This procedure will save time and items 4 and 9 can be skipped. Once Start button is pressed, partial calibrations can be aborted by pressing the Skip button. Important: Before to confirm a position, press F5 to test this movement in normal operation to avoid a bad calibration due to backlash.


4.7. Sample Tray This calibration is intended for alignment of sample sectors into the removal area. 1. Press F1 function or Start button. 2. Rotate Sample tray by using buttons or keys Q and E in keyboard until zone 1 is visible in the load area. 3. Re-adjust until sectorcan be loaded and unloaded through the loading area. 4. Press F3 function or Confirm button.

If instrument was already calibrated,Last button will position tray where last calibration was determined. This procedure will save time and item 1 can be skipped. Once Start button is pressed, calibration can be aborted by pressing theSkip button. Important: Before to confirm a position, press F5 to test this movement in normal operation to avoid a bad calibration due to backlash.

4.8. Reagent Tray This calibration is intended for alignment of reagents into the removal area. 1. Press F1 function or Start button. 2. Rotate Reagent tray by using buttons or keys Q and E in keyboard until Reagents 1 and 25 are visible in the load area. 3. Re-adjust until reagents1 and 25 can be loaded and unloaded through the loading area. 4. Press F3 function or Confirm button. If instrument was already calibrated,Last button will position tray where last calibration was determined. This procedure will save time and item 1 can be skipped. Once Start button is pressed, calibration can be aborted by pressing the Skip button.

4.9. Bar Code Reader 1. Press F1 function or Start button. 2. Install in reagent 1 position a vial with valid bar code. 3. Use buttons or letters Aand D in keyboard until vial isin front of BCR window. Use the 1-step option. 4. Press button or key R in keyboard and verify if code is read. 5. Repeat steps 3 and 4 until code is read. Lookfor the central position if code isread 50 . Pictus 700 Service Manual Rev.3

in a range of positions. 6. Press F3 function or Confirm button. 7. Install in sample 1 position a vial with valid bar code. 8. Use buttons or letters Qand E in keyboard until vial is in front of BCR window. Use the 1-step option. 9. Pres button or key R in keyboard and verify if code is read. 10. Repeat steps 3 and 4 until code is read. Look for the central position if code is read in a range of positions. 11. Press F3 function orConfirm button.

If instrument was already calibrated,Last button will position tray where last calibration was determined. Once Start button is pressed, partial calibrations can be aborted by pressing the Skip button.

4.10. ISE Module 1. Press F1 function or Start button. 2. Use buttons or letters Aand D in keyboard until tip is close to the centerof ISE loading window. Use 10-step or 1-step option asrequired. 3. Press F3 function or Confirm button. 4. Use buttons or letters S and W in keyboard until tip touch bottom cup. Use 10-step or 1-step option as required. 5. Press F3 function or Confirm button. 6. Use buttons or letters Aand D in keyboard until tip is close to the centerof ISE priming position. Use 10-step or 1-step option asrequired. 7. Press F3 function or Confirm button. 8. Use buttons or letters Sand W in keyboard until tip is in the bottom ofthe ISE priming position. Use 10-step or 1-step option asrequired. 9. Press F3 function or Confirm button. If instrument was already calibrated,Last button will position tray where last calibration was determined. This procedure will save time and item 2 can be skipped. Skip Once Start button is pressed, partial calibrations can be aborted by pressing the button. Important: Before to confirm a position, press F5 to test this movement in normal operation to avoid a bad calibration due to backlash.


Note: Last button does not act on vertical positions. This is so to prevent tip damage.

4.11. Photometer Calibration Photometer calibration consist of automatic adjust of gains for front, back and reference channels. Also, energy ratios front/reference and back/reference are evaluated. Ratios are used for absorbance calculations. Calibrations must be performed only when lamp is changed or filters are cleaned or replaced. Channel Ratio must be re-calculated about once every two weeks. When calibration or ratio evaluation is started, instrument ask for a new cuvette in position 1. When calibration is performed, error messages will be issued if gains are too high or too low. No conditions are established on ratios.

4.12. Sample sector definition For sector definition, select Mainte nance > Parameters > Sector definition , then define a new sector with a number and assign the STAT condition, if required. Be sure that number is not already defined in the column to the right. If so, first delete definition and then re- enter new definition, including STAT condition.


5.

Preventive Maintenance

5.1. Half-Yearly maintenance kit. Code: KitMaint40006 Quantity Code

Description Syringe Cavro 73804 for 2 VOSB1206 HS600 1 M400BH21/3 Peristaltic pump tubing 2 M400BH19W Washer filter 1 KITOIL4000 Lubrication Kit

1. Follow procedure Syringe diluter replacement (12.2.2) 2. Follow procedure Peristaltic pump tubing replacement (11.3.5) 3. Follow procedure Washer pumps filter replacement / cleaning (11.3.8) . You should replace the front reaction pump (FRP) and back reaction pump (BRP) mesh filters, and clean FWP and BWP mesh filters. 4. Follow procedure Lubrication procedure (5.5) 5. Follow procedure Fan filters cleaning (5.4)

5.2. Yearly maintenance kit. Code: KitMaint400012

Quantity Code

Description Syringe Cavro 73804 for 2 VOSB1206 HS600 1 M400BH21/3 Peristaltic pump tubing 2 M400BH19W Washer filter 1 KITOIL4000 Lubrication Kit 1 LAR12V20W Halogen Lamp 12V 2 OTDI700A Diaphragm for Drying pump 2 OTDI700L Diaphragm for Water pump 2 M40J14W Drying block 2 M400BH09W Peristaltic pump rotor 1 M4000TUH Tubing Sets 12 month

1. Follow procedure Syringe diluter replacement (12.2.2) 53 . Pictus 700 Service Manual Rev.3

2. Follow procedure Peristaltic pump tubing replacement (11.3.5) . Once you removed the tubing, and before placing the new one, you should replace the pump rotor following procedure Peristaltic pump rotor replacement (11.3.6) 3. Follow procedure Washer pumps filter replacement / cleaning (11.3.8) . You should replace the front reaction pump (FRP) and back reaction pump (BRP) mesh filters, and clean FWP and BWP mesh filters. 4. Follow procedure Diaphragm for drying and water pump replacement (11.3.7) . You should replace the diaphragm for the FWP, BWP, FDP, and BDP. Follow procedure


Tubing Sets 12 month (5.6) 5. Follow procedure Drying block replacement (11.3.4) 6. Follow procedure Lubrication procedure (5.5) 7. Follow procedure Photometer cleaning (6.2.1) 8. Follow procedure Halogen lamp replacement (6.2.5) 9. Follow procedure Fan filters cleaning (5.4)

5.3. ISE Half-Yearly maintenance kit Code: KitMaintISE40006 Quantity Code 1 VATUPINCH 1 M24M64/3

Description Pinch valve tubing ISE peristaltic pump tubing

1. Perform procedure ISE Pinch valve tubing replacement (13.2.7) 2. Perform ISE Pump tubing replacement (13.2.3)

5.4. Fan filters cleaning 1. Locate all the fan filters from the back and side panels of the equipment.


2. Remove, clean, and placeit again one at a time. Place the filters under the tap in order to clean it. Dried properly before placing.

5.5. Lubrication procedure Lubrication procedure should be repeated every six months. For units with continuous work that exceeds the 1500 analysis/day the procedure should be repeated every four months. Starting software version 1.8.1, an automatic warning will be issued whenthe lubrication becomes necessary. Use mineral oil SAE 15 or SAE 20. DO NOT USE MORE THAN TWO DROPS IN EACH OILING POINT, otherwise excess of oil could fall on sensors and belts and damage the system. Oil can be added with an hypodermic syringe. For reference, two drops is equivalent to 50 microliters. Remove cover and top desk as follows: a) Remove the six setting screws located on top of desk. b) Remove both arm covers and the small closing arm covers. c) Rotate arms until they point towards the center of the reagent tray. d) Remove rings and mountings of both washing stations. e) Carefully remove desk, front side first Remove also right side panel.


1. Bushing. Horizontal movement Remove two screws that hold upper limiter plate. Pushfirmly the vertical axis up. This will leave a gap between belt and washer of about two millimeters. With the hypodermic needle introduce oil in the gap, as deep as possible.

2. Bushing. Vertical movement, parallel rod.

Bearing. Vertical movement. 57 . Pictus 700 Service Manual Rev.3

3. Free pulley. Lower end, vertical belt. Be sure that the amount of oil is very small; otherwise it could spill on sensors. Oil drop should fall over the Teflon washer.

Bushings. Parallel rods. Washer. 58 . Pictus 700 Service Manual Rev.3

4. Bushing. Reagent tray axis. Use a pipette with a drop in the tip or a rod with cotton wetted with oil, wrapped at its end. Introduce the pipette/rod abovethe motor located betweenthe two cooling fans. Touch the upper end of bushing with rod or blow the oil standing at the pipette tip. If using rod with cotton, rotate byhand the tray and repeat procedure.



5.6. Tubing Sets 12 month 1. Unplug the equipment.

2. Remove the equipment back, front and side panels in order to have access to the washers and tip water pumps

3. You will need to replace the tubing from the washer, including the water suction pump tubing (pipes 16 to 19), water delivery pump tubing (pipes 11 to 14), reaction pump tubing (pipe 15), and drying block pump tubing (pipe 28). Also you will need to replace the DI water intake tubing from the DI water bottle (T022F and T022B).


4. Check the hydraulic diagram for more information regarding tubingconnection.


5.7. ISE Medica Maintenance Schedule The ISE Module has been designed to require very little operator maintenance. The only daily maintenance required is to run the cleaning solution after the last sample of the day or after 50 patient samples (automatically done), whichever is first, that will be automatically required by the software. Clean the sample inlet port with a cotton swab and DI water once per week. All other parts and expendables are replacement items (see schedule below). Use only MEDICA approved components. Recommended Component Replacement Schedule (low volume user) Li+ Electrode

6 months

Pump Tubing

6 months

Na+ Electrode

6 months

K+ Electrode

6 months

Cl- Electrode

6 months

Reference Electrode

6 months

Fluidic Tubing

12 months

Recommended Replacement Schedule (high volume user, greater than 100 samples/day) Li+ Electrode

3,000 samples

Pump Tubing

6 months

Na+ Electrode

10,000 samples

K+ Electrode

10,000 samples

Cl- Electrode

10,000 samples

Reference Electrode

10,000 samples

Fluidic Tubing

12 months


6.

Photometer

6.1. Verification and diagnostic 6.1.1.

Lamp voltage stability check

1. Check lamp voltage (12v) is steady (±1mV) during instrument operation. 2. Check lamp power supply output voltage (Meanwell S-40-12). Check connections, replace PL400273 in case it introduces the instability.

6.1.2.

Filter wheel calibration check

1. Log as service (Data > Log as > Service > passw ord 31415) 2. Go to Maintenance > Service > Calibration > Filter wheel . 3. Press start and check that peak for each filter and channel is centered (Ignore saturating channels)– see image below. 4. Filter 13 is blocking (no signal) and shows no main peak.

5. Choose Maintena nce > Pho tom ete r Ca libration

select summarize tab


Proper calibration is defined as: •

20000 < Front Ch, Back Ch, and Ref Ch < 480000

6.1.3.

Photometer and lamp alignment check

1. Turn on instrument. 2. Gently block filter wheel movement or go to maintenance->service->manual->filter wheel and press stop button, then rotate gently to choose a bright filter 3. Locate a paper on the back preamplifier cavity, checkalignment against the reaction tray main rod. Verify that the light should be round and constant intensity throughout its shape. See picture.

4.In case alignment not passes the criteria, perform lamp alignment (6.2.4).


6.2. Maintenance 6.2.1.

Photometer cleaning

1. 2. 3. 4.

Verify that instrument is turned off. Remove left side cover. Identify photometer and filter wheel. Use cotton swabs to remove dirt from filters surface.Rub gently until opalescence is removed. 5. After cleaning is complete, reinstall cover, turn the instrument onand allow it to warm up for 15 minutes. 6. NOTICE: Filter No. 0 is an opaque dummy, and requires no cleaning. 7. Perform procedure Photometer calibration (6.2.3)

6.2.2.

Adjustment of filter delays

Peristalt WARNING: This is a factory adjustment and it should not be modified unless a i

major servicing is required (motor replacement, sensor position adjust, etc.) Do not use if a filter is replaced. This procedure will reset preamplifiers gain, photometer calibration is further required.

1. Enter to Data > Lo g as > Service > passw Maintenance > Calibrati

ord 314 15 , then on > Filter wh eel .

2. Remove all cuvettes from light path. 3. Choose Reset gains and Reset delays . Set reset delays to 20, use Normalization by channel and then push Start . 4. Adjust Offset until all the signals peaks are almost in the center of the graphics. Noise must be present on filter # 13, otherwise you moved the Offset too much.


5. Adjust each filter Delay to center the three signal peaks (useStay on filter option).

6. Push Stop button when done. 7. Perform procedure Photometer calibration (6.2.3)

6.2.3.

Photometer calibration 67


Calibration is performed on four steps: - During the Initialization , instrument checks communication to the other modules. - Gain setting, adjusts internal signal levels to ensure linear operation of A/D converter. - Channel ratio, establishes the relationship between both front and backsample channels against reference channel. - Water absorbance, reads the water absorbance for all the filters. 1. Enter to Maintena nce > Photo meter Calibration 2. Push Start calibration . 3. Wait until finish.

.

4. 480000. Choose Summarize tabwill and check: 20000 < Front Ch, Back Ch, and Ref Ch < The software raise an error if this condition is not met.

6.2.4.

Lamp alignment & beam splitter calibration

WARNING: This is a factory adjustment and it should not be modified.

1. Remove both beam splitters, A (Reference channel) andB (Back channel), to achieve that first remove screws and then pull them like show pictures.


2. Center the lamp filament in the input lens, like show picture. Precaution: The lamp must be free without touching some part.


3. To modify the lamp position use these4 screws that allow move it in 3 axes.

4. Perform alignment check(6.1.3) and readjust lamp position if not pass the criteria acceptance. 5. Enter to Data > Lo g as > Service > passw 6. 7. 8. 9.

ord 314 15 , then Maintenance > Calibrati on > Filter wheel . Choose Reset gains. Do not check Reset delays . Push Start , wait until the channel signals start to appear, and pushStop . Go to Maintenance > C alibration > Lamp in tensity . Choose reading for filter #1 and push Start button.


Lamp intensity

window

10. Re-adjust of lamp socket to maximize readings on back channel and verify visually (Procedure 6.1.3). 11. Put beam splitter front channel (Picture ref B) and rotate it to achieve front channel maximum energy. Then fix its corresponding screw. 12. Put beam splitter back channel (Picture ref A) and rotate it to achieve reference channel maximum energy. Then fix its corresponding screw. 13. Perform procedure adjustment of filter delay (6.2.2) andPhotometer calibration (6.2.3).

6.2.5.

Halogen lamp replacement

1. Unplug the equipment.

2.

Remove the lamp access panel.


3. Remove the halogen lamp from the socket pushing the lever.

4. Place the new halogen lamp into the socket. Take care of the lamp polarity. The big terminal must goes up.


5. Close the lamp access panel and plug the equipment. 6. Turn on the software. Perform a system flush, and replace the first cuvette strip from both trays. 7. Perform a full photometer calibration from Mainte nance > Photom ete r Calibration .


Arm

7.


Probe dispensing pump check

1. Enter to Maintenance > Operation s choose movements tab, set Steps to 500 on pump water section. 2. Press A key, check dispensedvolume using a buret, 3. Check for clogs or identify the faulty pump in case specified range is not meet. (See section IV, hydraulics schematics).

7.1.2.

Probe robotics check

1. Reset instrument (press red pushbutton). 2. Gently move the probe from side to side and up to down checking movement is smooth without jams. 3. Ensure that neither flag or disctouch optocoupler sensor body. 4. Compare zero and verification led blinking against the other probe. 5. Swap front/back motor controllers boards. Check connections. 6. In case of step loss: Check for belt damage (e.g. cracked belt surface or missing teeth), loose pulley to rod joint or heavy motor.

7.1.3.

Probe level sensing check

1. Perform Level detection test M ( aintena nce > system tests > Level detection 2. In case probe stops on air (falsely detecting liquid level), • • • •

Check Check Check Check

).

lab neutral to ground mains below to 1V (both DC and AC modes). or replace probe. or replace level sensing board. or replace wiring from level sensing board to distribution board.


7.1.4.

Mixer checking

1. System must be flushed. 2. Log as service, Data > Log as > Se rvi ce > passw ord 3141 5 3. Access to Maintenance > Ope rations > Movem ents > Pump Water . Select the desired arm, check go to washing station, and push button A. 4. Access to Maintenan ce > S ervic e > Manual >Vertical , and select the desired arm. 5. Place a floating particle of aroundhalf millimeter in the washing station. You can use a rubber particle.

6. Push Turn on vibrator button. 7. Check that the particle moves in a circle around the washing station.

8. Remove the particle.


7.2. Maintenance 7.2.1. 1. 2. 3. 4. 5.

Probe replacement

Remove probe assembly plastic cover. Disconnect both electrical and hydraulic connections. Release setscrew in the disk that fix probe. Install the mixer assembly on the new probe and install the new probe. Perform mechanical calibration procedures to adjust: Arm and reaction tray (4.2),

arm and washing station(4.4), arm and sample (4.5), arm and reagent tray(4.6), and ISE module (4.10). 6. Perform


Mixer checking (7.1.4).

7.2.2.

Removal of complete arm assembly

1. Remove the equipment desk. 2. Disconnect the arm tubing (tubing connector is located inthe middle way of arm and diluter end).

3. Remove the three biggest screws from the arm assembly.

4. Gently pull it up and disconnect J1, J2 and J3 from the distribution board.


7.2.3.

Vertical arm belt replacement and tension belt adjustment

*Belt re placemen t

1. Perform Removal of complete arm assembly (7.2.2) . 2. Release screw from pulley support. See picture 3. Move pulley support and remove the vertical verification sensor andrelease the guiding shaft to loose and remove the belt. *T ensio n b elt a djustment

1. Perform Removal of complete arm assembly (7.2.2) . 2. Identify the vertical belt. 3. pulley adjust belt tension. 4. Move Perform Beltsupport tensionto measurement and value settings (3.4.6) .


Model for instruments SN:_ _ _ _ 4 0 _ _

Model for instruments SN:_ _ _ _ 4 1 _ _ 79 . Pictus 700 Service Manual Rev.3

7.2.4.

Horizontal belt replacement and tension belt adjustment

*Belt re placemen t

To remove the belt attached to the motor 1. 2. 3. 4.

Perform Removal of complete arm assembly (7.2.2) . Release motor screws. See picture Remove the verification sensor board. Remove the belt.

To remove the belt attached to the movement shaft 5. Release the belt attachedto the motor (as mentioned onsteps 1 to 4). 6. Release the setscrew. Seepicture. Only for SN__ _ _ 40 _ _. 7. Use the hole to rotate the eccentric to release the belt attached to the movement shaft. Only for SN_ _ _ _ 40 _ _. 8. Release The belt attached to the shaft. *T ensio n b elt a djustment

Model for instruments SN_ _ _ _ 40 _ _ 1. Perform Removal of complete arm assembly (7.2.2) . 2. Release motor screws. See picture 3. Release the setscrew. See picture 4. Use the hole to rotate the eccentric to adjust the belt tension attached to the movement shaft. 5. Perform Belt tension measurement and value settings (3.4.6) . 6. Fix setscrew. 7. Move motor to adjust the tension belt attached to he t motor. 8. Perform Belt tension measurement and value settings (3.4.6) . Model for instruments SN_ _ _ _ 41 _ _ 1. Perform Removal of complete arm assembly (7.2.2) . 2. Release motor screws. See picture 3. Move motor to adjust the tension belt attached to he t motor. 4. Perform Belt tension measurement and value settings (3.4.6) . .



Model for instruments SN:_ _ _ _ 4 0 _ _ 81 . Pictus 700 Service Manual Rev.3



7.2.5.

Adjust and verify probe optical collision sensor (M400-P317 Head board)

1. Tip collision is detectedby the interruption of the light emitted by an optical sensor generated by the movement in the collision flag. When the flag is moved, the hole in the flag will not be aligned anymore with the optical light path of the sensor and the collision will be detected.

Optical collision sensor

Sensor light path

Flag hole

2. The collision flag holemust be aligned with the sensor optical path. You must center vertical and horizontal sides of the collision flag with the optical sensor. Collision flag must be equally distant from the sensor sides.

3. To achieve alignment, you will needto loosen board screws and move the board up and down. Small adjustments can be obtained. Once finished, tighten the screws.


4.

If you can’t perform the alignment procedure, you will need to use flat nose pliers in order to adjust the collision flag.

5.

To verify a correct adjustment go to Maintenance > service > Manual > Vertical Turn on and off vibrator quickly and check that the collision led (DL4) is always turned off.


Reaction Tray

8.

8.1. Verification and diagnostic 8.1.1. 1. 2. 3. 4. 5. 6.

Tray movement check

Reset instrument. Gently move the tray side to side checking movement is smooth without jams. Move the tray from side to side checking controller leds. Ensure that neither flag or disc touch optocoupler sensor body. Swap motor controllers boards. In case of step loss: Check for belt damage (e.g. cracked belt surface or missing teeth), loose pulley to rod joint or heavy motor.

8.2. Maintenance 8.2.1.

Complete removal of reaction tray

1. Remove reaction tray cover

3. Remove the four screws and the plastic reaction tray ring

2. Remove cuvette holder

4. Remove the two screws for the cuvette holder lock 85


5. Remove center screw and pull up ht e reaction tray support

8.2.2.

Removal and replacement of reaction tray heater

1. Perform procedure Complete removal of reaction tray (8.2.1) 2. Remove the four screws located onheat sink to access to heater

8.2.3.

Removal and replacement of reaction tray fan

1. Perform procedure Complete removal of reaction tray (8.2.1) 2. Remove air director from the fans and remove fans.

8.2.4.

Reaction tray belt replacement and tension belt adjustment

*Belt re placemen t

1. Release screws. See picture. 2. Move motor and remove the belt. *T ensio n b elt a djustment

1. Release screws. See picture. 2. Move motor to adjust the tension belt 3. Perform Belt tension measurement and value settings (3.4.6) .



9.

Reagent Tray


Tray movement check

1. Reset instrument. 2. Gently move the tray side to side checking movement is smooth without jams. 3. Move the tray from side to side checking controller leds. 4. Ensure that neither flag or disc touch optocoupler sensor body. 5. Swap motor controllers boards. 6. In case of step loss: Check for belt damage (e.g. cracked belt surface or missing teeth), loose pulley to rod joint or heavy motor.

9.2. Maintenance 9.2.1. 1. 2.

Removal of complete reagent cooler assembly Remove instrument deck. Remove instrument right side panel and rear panel.

3. Remove the gas spring

4. Remove instrument cover


5. Remove tensor´s screw.

6. Remove detachable lateral bar.

7. Remove the supply connection and the data connection of the fridge controller (see three arrows).

8. Removing the drain condensation tubing from the fridge. See hydraulic diagram.


9. Identify the fridge assembly inside the instrument. On the right side of the picture, you will see two post to support guiding wheels for the sample tray metal ring.

10. Identify the first post located on the right of the fridge assembly, perform marking using a permanent marker. The mark should be a straight line joining both the positioning rod and the flat surface as shown in the picture. This is to be able to locate this part exactly to the same position when re-assembly.

a


11. Release the setscrew to be able to rotate the post.

12. Identify the hole and use an allen wrench to rotate the post about 90o, this will turn an eccentric piece that will release the tray.

13. Release the pulley using an hex wrench and a 10mm wrench from the other side.


14. Remove the belt by gently sliding out of the big pulley of the sample tray.

15. Remove the sample ring by slowly and gently rotating the sample ring counterclockwise while lifting it.

16. Remove the upper setscrew


17. Use a thin pin and a hammer to hit and remove the locking pin of the white plastic support.

18. Remove the plastic support

19. Remove the white ring.


20. Identify the four screws you will need to remove to separate the fridge from its support.





24. Identify the condensation tubing of the fridge.

25. Gently pull the fridge (the white part that has attached two square pipes with fans). Before pulling from the fridge, it is necessary to release the condensation tubing (shown in previous step) and also check if it is necessary to disconnect any other wire from a sensor before pulling the fridge.


9.2.2.

Removal of Peltier thermoelectric devices

1. Perform procedure Removal of complete reagent cooler assembly (9.2.1) . Notes for checking Peltier devices: As a Peltier device varies its conductivity against the temperature and a current produces an internal temperature gradient, required instrument for a lab measurement is an AC-O. For field service purposes a DMM is enough to check it: • • • •

9.2.3.

Use a DMM on the lowest resistance range. Check not for shorts nor open Peltiers. Compare device against others on the cooler assembly Measure device in both directions– Check that resistance varies against the time.

Access to reagent verification sensor

1. Perform procedure Removal of complete reagent cooler assembly (9.2.1) . 2. Identify home/verification sensor assembly

9.2.4.

Reagent tray belt replacement and tension belt adjustment

*Belt re placemen t

1. Perform procedure Removal of complete reagent cooler assembly (9.2.1) . 2. Remove reagent verification sensor and motor. 96 . Pictus 700 Service Manual Rev.3

3. Remove belt. *T ensio n b elt a djustment

1. Release screws. See picture. 2. Move motor to adjust the tension belt. 3. Perform Belt tension measurement and value settings (3.4.6) .

9.2.5.

Cooler heating fans replacement

1. Perform procedure Removal of complete reagent cooler assembly (9.2.1) 2. Remove fan screws. 3. Fans will be mounting with air flow direction to outside of the instrument.


BOTTOM VIEW 4. To see fans PN go to spare part list.

Note: The only difference between fans is length wire.

9.2.6.

Procedure for reagent fridge temperature sensor replacement.

1. Perform procedure Removal of complete reagent cooler assembly (9.2.1) 2. Use a soft cloth on the table to protect against scratchings the delicate surfaceof the fridge. Put the fridge upside down.

3. Disconnect the sensor (see flat wire is disconnected from the board).

4. There are three screws holding each square pipe. Slide the fridge towards you to be able to access the screws (the screws are is located of chamber where normallyinside located the reagent tray) and remove the three screws. Then lift the square pipe that has attached the controller board to access peltier elements as 98 . Pictus 700 Service Manual Rev.3

shown. Notice that peltier elements are mounted in this fashion: Graphite sheet + Peltier element + Graphite sheet. The graphite sheet improves thermal conductivity between peltiers and fridge surfaces. Take care of the graphite sheets, because are very delicate and easy to break. In case you break a graphite sheet replace it and clean properly with alcohol the surface of the peltier elements to avoid any residues on them.

5. During the release of the screws attached to the pipe, take care of the support of each screw.

6. Identify the temperature old sensor, remove.

7. Identify the new sensor; it should have two screws and two white washers.


8. Clean the parts properly using gently and utility knife.

9. Apply a layer of silicone sealant.

10. Layer should cover completely the surface of the slot.


11. Screw the sensor do not force the screws, just screw to ensure sensor is in proper contact with the metal surface.

12. Final view of the sensor mounted.

13. Cover the sensor with a generous layer of silicone sealant.


14. Final view of the sensor mounted with the silicone sealant.

15. Identify the screws supports and position them properly on the fridge

You will receive spare graphite sheets to replace the damaged ones. Remember to mount the peltier elements with one graphite sheet on each side. Before mounting graphite sheets, clean both sides of peltiers with alcohol. You can use a drop of thermal grease to temporally glue the graphite sheet and ease mounting. •

• •

•

WARNING!!: When reassembling the fridge back to the fridge support, take care that shaft should be properly centered on its hole before tightening the screws (See pictures below)


9.2.7.

New reagent tray upgrade

1. Together with the new reagent tray, the upgrade kit comes with 1 setscrew, 1 screw, and 1 adapter.

2. Remove the old reagent tray.

3. Remove the setscrew.


4. Place the new setscrew provided with the upgrade kit.

5. Place the adapter

6. Place the screw provided with the upgrade kit. Do not over tighten the screw.

7. Now you can start using the new reagent tray.


10. Sample tray 10.1. Verification and diagnostic 10.1.1. Tray movement check 1. Reset instrument. 2. Gently move the tray side to side checking movement is smooth without jams. 3. Move the tray from side to side checking controller leds. 4. Ensure that neither flag or disc touch optocoupler sensor body. 5. Swap motor controllers boards. 6. In case of step loss: Check for belt damage (e.g. cracked belt surface or missing teeth), loose pulley to rod joint or heavy motor.

10.2. Maintenance 10.2.1. Sample tray belt replacement and tension belt adjustment *Belt re placemen t

1. Release the two ball bearings 2. Remove the verification sensor disk. *T ensio n b elt a djustment

1. Release the two ball bearings. 2. Move ball bearings to adjust tension belt. 3. Perform Belt tension measurement and value settings (3.4.6) .



11. Washer 11.1. Troubleshooting Symptom Corrective Action At the end of wash cycle, tiny water droplets Verify that all pumps are working. are in the cuvette walls Verify that no tubing are clogged Replace drying block Calibrate washer unit position. High cross-contamination

Identify cross-contaminants and methods in the Table of interferences Increase the wash volume Increase the number of wash cycles

set

11.2. Verification and diagnostic 11.2.1. Washer hydraulics check 1. See washer hydraulics schematics 2. Log as service (Data > Log as > Service > passwo

31415), then choose . 3. Dispensed volume shouldrange from 400uL to 700uL depending on method settings. - When volume is low on all pipes, check/replace peristaltic pump tubing or log maintenance > System test > washer hyd

rd

raulics

as supervisor and checkMaintenanc e > Se rvic e > P arameters >

es . - When volume is low on a given pipe, check for twisted tubing, clogs, or dirtiness. 4. Aspiration level should range from 2uL to 5uL, when higher than specified on every pipe, check aspiration pump, when higher than specified on a specific pipe, check for clogs, dirtiness or twisted tubing. Instrumental parameters Washing station section > front/back volum

11.2.2. Dispensing hydraulics check Note: Dilution test should be performed with a sample of Potassium Chromate of 2 g/l in acidic solution. Use as reagent the tip washing solution. For a final volume of 4/400, CV should be less than 1.5% When no potassium chrom ate is a vailable , te st settings can be m odified to use cho cholesterol standard as sample.

1. 2. 3. 4.

leste rol reagent and

Clean or replace water mesh filter (see hydraulic schematics). Remove syringe and check syringe glass to plunger contact is tight. Perform dilution test (Maintena nce > system test > d ilution ). During the test observe the following: 107


Check probe tip during operation not for droplets (e.g. dirty probe or damaged Teflon coating). Check for air bubbles on defective tubing joints. Check for bubbles on syringe plunger edge during aspiration operation (damaged plunger). Check / replace probe. Check beam alignment on cuvette. Check for twisted/clogged tubings. If no faulty tubing is identified, externallyconnect each tubing and repeat the test to identify a faulty (puncture) tubing. •

• •

5. 6. 7. 8.

Note: When no faulty tubing is identified, use the tubing kit to externally bypass each tubing and repeat the test to isolate the faulty one.

11.2.3. Washer mechanical check 1. Reset instrument (press red pushbutton). 2. Gently move the wash head up and down to check that movement is smooth without jams. 3. Swap controllers. 4. Ensure that neither flag or disc touch optocoupler sensor body. 5. Check connections. 6. In case of step loss: Check for belt damage (e.g. cracked belt surface or missing teeth), loose pulley to rod joint or heavy motor.

11.3. Maintenance 11.3.1. Removal of complete washer 1. Remove the wash head by removing its two screws. 2. Disconnect J1, J3, J4 and J5 from distribution board. 3. Identify and remove the two biggest screws located on washer fixture.


11.3.2. Adjust washer collision sensitivity 1. Identify collision sensitivity screw (the bigger one). Rotate clockwise to reduce washer collision sensitivity.

11.3.3. Washer belt replacement and tension belt adjustment *Belt re placemen t

1. Release nut of pulley support. See picture. 2. Remove the belt. *T ensio n b elt a djustment

1. Release nut of pulley support. See picture. 2. Move pulley to adjust belt tension. 3. Perform Belt tension measurement and value settings (3.4.6) .


11.3.4. Drying block replacement 1. Unscrew the drying block. 2. Screw the new one. Take care of the drying block position.

3.

Perform the washer calibration procedure.

4. Once replaced, proceed to reset cycle counter in Maintenanc e > Operation s > Wear .


11.3.5. Peristaltic pump tubing replacement 1. Pull fittings up and out of bracket.

2. Pull tube out of its lodging rotating by hand the pump rotor if necessary.

3. Clean the mesh filter.


4. Insert new tube on the fittings. 5. Push tube inside of its lodging. Turn slowly rotor by hand until tubing is properly lodged.

6. Once replaced, perform a washer volume calibration from Maintenanc e > Washer Volume Calibration . Push “Reset to default values”. 7. Proceed to reset cycle counter in Maintenanc e > Operation s > Wear .


11.3.6. Peristaltic pump rotor replacement 1. Remove the peristaltic pump tubing following the peristaltic pump tubing replacement procedure.

2. Locate the rotor set screw.

3. Using an allen key metric 2, unscrew the rotor set screw.


4. Remove the pump rotor. 5. Place the new pump rotor. The shaft flat position should point up.

6. Tighten the set screw. 7. Insert the pump tubing following the peristaltic pumptubing replacement procedure. 8. Once replaced, perform a washer volume calibration from Maintenanc e > Washer Volume Calibration . Push “Reset to default values”. 9. Proceed to reset cycle counter in Maintenanc e > Operation s > Wear .


11.3.7. Diaphragm for drying and water pump replacement 1. Unplug the equipment.

2. Remove the equipment back panel.

3. Identify the pump which the diaphragm will be replaced


4. For the 2 pumps in the left of the photo (FDP and FWP), in order to have access to the pump head, you will need to remove the pump from the mounting base. Using a screwdriver remove the 3 mounting screws.


5. Remove the 4 pump head screws.

6. Carefully remove the pump head.

7. Turn the diaphragm counterclockwise inorder to remove it.


8. Place the new diaphragm and close the pump head. 9. Place the pump in position. 10. Once replaced, proceed to reset cycle counter inMaintenanc e > Operation s > Wear .


11.3.8. Washer pumps filter replacement / cleaning 1. Unplug the equipment.

2. Remove the equipment back panel.

3. Identify the pump which the filter will be replaced/cleaned. Due to biological risk, we recommend to replace the FRP and BRP mesh filters. FWP and BWP filters can be cleaned.


4. Replace or clean the desired mesh filter.


12. Diluter 12.1. Verification and diagnostic 12.1.1. Diluter belt check

1. 2. 3. 4.

1. Turn off instrument. 2. Identify diluter belt. 3. Gently rotate belt pulleyand use a mirror to check for any belt damage (e.g. cracked belt surface or missing teeth). Initialize instrument. Enter to Data > Log as > S ervice > passw ord 31415, then enter to Maintenance > Service > Manual select temperature tab. Select the temperature controldevice. Initialize temperature controllerand set temp value. Press read temperature button. In case no value is shown, review errors.log file for further details.

12.2. Maintenance 12.2.1. Diluter valve replacement 1. 2. 3. 4. 5. 6.

7. 8.

9.

Remove as much fluid as possible from system flush tip without the water bottle. Initialize the diluter from Mainten ance > Se rvic e > Manual > Diluter Move the plunger to the bottom of travel from Maintenanc e > Service > Manual > diluter .

Remove the syringe and tubing. Remove the two socket head screws on the front of the valve, then remove the valve from the pump. Install the new valve by placing it on the front panel so that the screw holes line up. The valve coupler fitting mates to the valve motor shaft. The shaft should be in the correct position Replace the valve screws but do not tighten completely. Install the syringe and pull the syringe plunger until it is aligned with the carriage. Align the valve using the plunger as a guide, and tighten from 1/4 to 1/2 turn after the screws contact the valve body. Pull the syringe plunger all the way into the carriage and secure by tightening the plunger lock screw.


12.2.2. Syringe diluter replacement 1. 2. 3. 4. 5. 6.

Remove the plunger lock screw. Install the syringe as shown in Figure 2-7, following these steps: Screw the syringe into the valve. Pull the syringe plunger down to the plunger holder assembly. Align the plunger button through hole to the carriage mounting hole. Slide the plunger lock screw through the plunger button and fasten to the carriage. Note: Make sure the plunger lock screw is securely tightened and the plunger

button is free to move on the plunger lock screw.



12.2.3. Special replacement procedure for Diluter 1. Set the new diluter accordingly: for Front diluter (left located) Rotary switch set to ID = 5. J4 termination jumpers installed for Back diluter (right located) Rotary switch set to ID = 6 J4 termination jumpers not installed 2. 3. 4. 5. 6.

Turn the instrument off. Install the new diluter (front/back) Disconnect the other diluter (back/front) Turn the instrument on. Enter to Data > Lo g as > servic e > 3 141 5 then Maint enance > servic e > Manual then choose diluter tab and the proper front / back diluter installed. 7. Press Set baud rate to store the proper baud rate into the new diluter EEPROM. 8. Turn off the instrument. 9. Wait for 10 seconds. 10. Reconnect the other diluter.


13. ISE 13.1. JS ISE Troubleshooting ERROR Kit not installed Kit expired Empty kit Invalid kit

ION Any

DESCRIPTIÓN No startup

Any Any Any

No startup No startup No startup

Error in filling Any

No data acquired or calibration performed

CORRECTIVE ACTION Install a valid kit or IC Key from ISE pack is not installed. Replace ISE pack Replace ISE pack Utilize ISE pack designed for your instrument and/or country.

- Inspect valve tubings, remove from pumps and rub with fingers.

- Check that all tubings don’t have obstruction.

- Check pressure in pack bottles (13.2.6) and verify pinch valve time (500 factory value). Dispensing Std A or B should be 180ul. - Perform maintenance procedure. - Check leaks and kinks in tubing electrodes. - Change cup or reference electrode. Error in emptying

Any

No access to the Inspect peristaltic pump and tubing. Check following sample leaks and kinks in tubing or no calibration and electrodes.

Na unstable

Sodium

No stable plateau reached in samples and calibration. Slope out of the allowed range

(*)

K unstable

(*)

Potassium

Electrode deteriorated. Erroneous slope after a calibration. Some samples can display this message. Only check if it persists for many samples. Clean electrodes with Sodium conditioning solution. No stable Electrode deteriorated. Erroneous threshold plateau reached in module. in samples and Some samples can display this message. calibration. Only check if it persists for many samples. Slope out of the Perform an electrode conditioning with allowed range serum to recover the electrode (See user manual for more details)


Cl unstable

Sodium

(*)

ISE no answer Timeout

------

No stable plateau reached in samples and calibration. Slope out of the allowed range Erroneous date or module missconnection.

Electrode deteriorated. Erroneous threshold in module. Some samples can display this message. Only check if it persists for many samples.

- Communication

-

interrupted.Check cables and boards regarding ISE (See electronic diagram). Error in filling error while is running auto wet (every 30 minutes). Perform corrective action for error in filling.To recover from this error turn off the instrument from the main switch located in the rear of the instrument.

ADC error

Any

ADC message

error

- Electrode deteriorated. Verify in previous calibrations if all slope for Cl, K, Na give low change reference electrode. Otherwise change only the electrode with low slope.

13.2. Maintenance 13.2.1. Electrode removal or cleaning 1. 2. 3. 4. 5.

Open small back door in rear panel of instrument with the aid of a screwdriver. Remove the fixing screw and flip the hinged ISE module out of the instrument. Loosen but do not remove two fixing hexagonal socketscrews as shown in figure. They are located on the back side of ISE module. Move to the right the electrodes package sothe electrode contactsare freed from the connecting clips. 6. Pull electrodes down.


7. Silicon rings between electrodes can be washed and re-used

8. If necessary remove electrode and clean capillary tubing with cleaning solution. 9. Rinse with distilled water. 10. To re-install be sure to preserve the ordering: Cl, K, and Na from top to bottom. 11. Press firmly the whole electrode package and re-adjust screws on the back. 127 . Pictus 700 Service Manual Rev.3

12. Push the electrode frame to the right so electrode contacts are holded by clips.

13.2.2. Pinch valve unclogging

1. Pull tubing as indicated by arrow in picture. 2. Press corresponding Standardbutton at the same time. (Maintenan ce > Operatio ns > I SE ). 3. Once outside valve, rub with fingers until standardflows normally. 4. Re-install in pinch valveby pushing tubing and pressing delivery standard buttonat the same time. 5. Perform procedure for valves A and B.


13.2.3. Pump tubing replacement 1. Open lower compartment in the rear panel where ISE pack and peristaltic pump are located. 2. Rotate peristaltic pumptubing rotor and gently pull tubing end as shown. 3. Remove connectors. 4. Re-install new tubing. Besure that connecting tubing hasno kinks. Pass them through holes, if necessary

13.2.4. Electrode recovery Sometimes, mainly after measuring many consecutive urine samples or if cleaning procedure is repeated several times, slopes might decrease to values well below the stability threshold (30). Before any attempt to replace electrodes, load system with any serum sample and leave it in contact with electrodes for about 30 minutes. Next, empty module and calibrate. Complete procedure should be as follows: 1. 2. 3. 4.

Select Mainten ance > Operations > ISE Press Startup button and wait until calibration is finished. Put serum in prime position. Complete cuantity of minutes on electrode conditioning box,filled for 30 minutes. In difficult cases, system can be left filled with serum, overnight.

Start button. 5. Presspress 6. Then Startup button. 7. Wait until calibration ends. Checkfor slopes in the Coordinates display. Ifthey have not recovered, electrode replacement will be necessary.


13.2.5. Sodium electrode conditioning When Na+ calibration slope goesdown (below 35), Na+ instability or more than15 days elapsed since treatment messages are displayed, a Na conditioning cycle is recommended, To perform it follow next steps:

Mainten ance > Operations > ISE 1. 2. Select Press Na conditioning button and wait until the operation is finished. 3. Perform a calibration and check slope value.

13.2.6. Pump pressure adjustment This procedure should be performed by trained service engineer. 1. Remove Rear panel and right side panel (front view). 2. Locate pressure connector corresponding toStandard A delivery. In the ISE pack side, is the connector adjacent to the pack green connector. In the opposite side is the upper female white connector attached to pressure retainer valve.

3. Disconnect female Lugerconnector. Be sure that pack is connected and connector corresponding to Standard B is connected.

4. Attach pressure meter equiped with male Luger connector to the corresponding female connector. 130 . Pictus 700 Service Manual Rev.3

5. Locate pressure controller board by inspecting the right side of the instrument.

6. Select Maintenanc e > Operatio n > IS E 7. Deliver Standard A at least twice. Read pressure on manometer. Adjust preset on board to a value of 100 mmHg of pressure. Before any readjustment deliver Standard A. 8. Repeat procedure until pressure is stable. 9. Disconnect manometer, reinstall connector and covers.

13.2.7. Pinch valve tubing replacement 1. Remove ISE pack. 2. Pull tubing out of the valve and at the same time, from Maintenance > Operations > ISE, press corresponding Standard button.


3. Once outside valve, remove the tubing from both ends. 4. Place the new tubing. 5. Re-install the new one in pinch valve by pushing it and pressing delivery standard button at the same time. 6. Install ISE pack.

13.2.8. ISE pack removal 1. Disconnect pressure lines from pack. 2. Disconnect delivery lines from instrument. 3. If no pack will be installed immediately, attach pressure lines from instrument to inlets of standards in the instrument. This will prevent dust and contaminants from entering the delivery lines. Also, if module is turned on, any remaining liquid in the system will be eliminated through the drain circuit. 4. When pack is removed, discard according to your localregulations. IMPORTANT: Always disconnect pressure lines from pack before disconnecting delivery lines from instrument. This will avoid any pressure in pack vials that may cause spillage through delivery lines.


ISE Pack connections

CAUTION: Damage in the peristaltic pump tubing may cause spillage of corrosive liquids and instrument damage. Replace tubing when instrument warns that safe number of cycles has expired.

13.2.9. ISE pack installation 1. Before installation ofa new pack, remove the old one. 2. Open rear door where ISE pack must be located. 3. Remove pack cover. Also remove Cleaning solutionand Urine diluents vials. These two solutions fit into reagent tray in positions as reported in Functional Parameters. 4. Standard A (green coded) and Standard B (orange coded) have connectors which attach to instrument with female luer-lock terminals. In turn, pressure lines from instrument attach to luer-lock connectors in pack. 5. When pack is opened, delivery lines are attached to pressure connectors, each one to its corresponding Standard.

Reagent

6. If instrument is new and no pack was installed before, pressure lines are attached to inlet hoses. Disconnect them. 7. Disconnect delivery lines in pack and connect them to instrument. 8. Connect pressure lines from instrument tocorresponding color coded terminals in the pack. 9. Connect micro-chip to J9connector in the instrument. CAUTION: line is be connected pack before connecting to instrument and instrumentIfispressure ON, liquid will deliveredtoand spilled


When pack is installed, be sure that absorbing sponge located below peristaltic pump is correctly positioned.

13.3. ISE MEDICA Module WARNING:

Never leave the Module without a pack or connected to an empty one. Cleaning solution should always be on tray. Follows the maintenance schedule.

13.3.1.Overview MEDICA ISE Module includes ion-selective electrodes and three peristaltic pumps. The ISE module measures the concentration of Li+, Na+, K+, and Cl- in serum, plasma and diluted urine. An integral sample entry port is positioned on top of the ISE Module, were the instrument's probe dispenses the sample. This compact design allows for small sample size and fast operation.

The ISE Module houses snap-in, snap-out electrodes which connect directly to an electronic board within the ISE Module. This eliminates the need for cables and minimizes electrical noise. Samples and calibrators are positioned in front of the electrodes by three peristaltic pumps. Two separate pumps move Calibrant A and Calibrant B into the ISE 134 . Pictus 700 Service Manual Rev.3

Module’s sample entry port and a waste pump positions samples and calibrants in front of the electrodes. The sample is deposited by the analyzer's probe into the sample entry port. After each sample measurement, calibrant is positioned in front of the electrodes for a single-point calibration. The removal of protein build-up on the electrodes and fluid path is accomplished by the use of cleaning solution. Cleaning solution is placed in a reagent position on the analyzer sample tray, aspirated, and deposited into the sample entry port by the analyzer's probe.

13.3.2. ISE Installed in the instrument

13.3.3.Electrodes Electrodessolution, are maintenanceree. Electrode packages are markedwill withbeanrequired “Install-by date.” Cleaning aspirated ffrom the analyzer reagent position, every time that the software is open, after a measuring cycle and every 50 samples, to minimize protein buildup in the fluid lines and electrodes. A pump calibration is performed each day. A two-point calibration is performed every 8 hours, and after electrodes cleaning. To ensure 135 . Pictus 700 Service Manual Rev.3

reliable operation, the ISE Module performs calibrant sipping every 30 minutes, beginning after the last sample is run. This function is completely controlled by the ISE Module. The ISE Module utilizes a double-junction reference electrode. The reference electrode is filled with saturated KCl. If the concentration of the reference electrode reservoir drops below 3.0M KCl, serious errors will result in the measured electrolyte concentrations. The reference electrode contains a small red sphere in the reservoir which normally resides on top of the filling solution. If the sphere begins to sink, the reference electrode must be replaced. When measuring urine, the sample will be automatically diluted by the instrument (1:10 with MEDICA urine diluent).

13.3.4. Fluid Management The sample is aspirated by the analyzer from a sample cup and dispensed into the sample entry port on top of the ISE Module, by the probe. The sample is then positioned in front of the electrodes for measurement. Four solutions are required to operate the ISE Module.


13.3.4.1. Calibrant A Used in both two-point and single-point calibrations for serum sample analysis. Calibrant A is pumped into the sample entry port by the Calibrant A pump and then positioned in front of the electrodes by the waste pump. Calibrant A solution is also used for Pump and Bubble Calibration. Calibrant A Solution Li+ 1.0 mmol/L Na+ 140 mmol/L K+ 4.0 mmol/L Cl- 125 mmol/L

13.3.4.2. Calibrant B Used in two-point and single-point calibrations for urine sample analysis. Calibrant B is pumped into the sample entry port by the Calibrant B pump and then positioned in front of the electrodes by the waste pump. Calibrant B Solution Li+ 0.4 mmol/L Na+ 70 mmol/L K+ 8.0 mmol/L Cl- 41 mmol/L

13.3.4.3. Cleaning Solution Will be required every time that the software is open, after a measuring cycle and every 50 samples, to minimize protein buildup in the fluid lines and electrodes. It must be used more frequently if the ISE Module performs more than 50 sample measurements per day. 100 μL of cleaning solution is aspirated by the analyzer from a reagent position and dispensed into the sample entry port. The cleaning solution in the reagent container must be covered to eliminate evaporation. NOTE: Medica pepsin/HCl cleaning solution must be prepared every four weeks and stored at 4º C.

13.3.4.4. MEDICA Urine Diluent. Urine samples will be automatically diluted to perform urine measurement 1:10 with MEDICA urine diluent.


13.3.5. Test Ranges Analyte

Units

Test Range Min.

Test Range Max .

(Whole blood, serum, plasma) Li+

mmol/L

0.20

3.50

Na+

mmol/L

100.0

200.0

K+

mmol/L

1.00

10.00

Cl-

mmol/L

50.0

150

Na+

mmol/L

10

500

K+

mmol/L

5

200

(Urine)


Cl-

mmol/L

15

400


13.3.6. ISE Pack Installation

The new ISE Pack is provided as shown.

Remove the pack from it protecting box.


Identify the connection caps.

Remove the caps from the connections. On reagent pack replacement, the rubber caps can be used to cap the container prior to disposal.

From Maintenanc e > Operation s > ISE push Change reagent Pack. Waits until the pop up window shown.


Gently push the tubing terminal until it clamps to the pack.

Push Ok on the pop up window and wait until operation finish.

13.3.7. ISE Pack Removal From Maintenanc e > Operation s > ISE push Maintenance. This command disables the automatic wet. Never leave the Module in this status with the reference electrode in place for more than an hour after a Empty is initiated, as KCl will diffuse out of the reference electrode. Over time, the KCl will clog the flow path.


Press the yellow button on top of the tubing connector and pull gently.

13.3.8. MEDICA Operational Parameters Maintenanc e > Se rvic e > P arameters > Instru

ment al > I SE.

ISE parameters must be set in order to ensure a proper operation. Parameter Urine Diluent Factor Prime Idle Time (minutes) Prime Repetitions Serum volume (ul) Urine volume (ul) Extra volume (ul) Air Gap

Value 10 20 1 100 10 50 100

13.3.9. Replace electrodes procedure 1. Remove the back cover 2. Remove set screw to release the module.


3. Move the module outside of the instrument. 4. Press replace electrodes button from Maintenance > Operatio pop up to change the electrodes.

n > ISE

and wait the

5. Depress the compression plate andremove all electrodes, including thereference electrode from the ISE Module. 6. Put the new electrodes in the module after that confirm the Pop up. 7. Move the module in position and fix with the set screw. 8. Put the back cover.


13.3.10. Shutdown Procedure: Preparing the ISE Module for Storage If the laboratory plans to store the Instrument, or discontinue the use of the module, the following steps should be performed with the ISE Module: Before removing the electrodes, they should be cleaned using the cleaning solution and then 3 Purge A cycles should be run. Then, fromMaintenanc e > Operation s > I SE , push Maintenance to purge the ISE Module fluid path. Reference, Na+, Cl- electrodes

• Depress the compression plate and remove all electrodes, including the reference electrode from the ISE Module. • Place the Na+ and Cl- electrodes into individual sealed bags. • Reinsert the yellow flag into the reference electrode flow path line, if available. Then put the electrode into individual sealed bag. K+ and Li+ electrodes • Aspirate a small volume of Calibrant A from the top port of the reagent pack into a syringe fitted with a blunt needle. • Inject sufficient Calibrant A into the lumen of the K+ and Li+ electrodes until fluid fills the lumen. • Cover both ends of the lumen (both sides of the K+ and Li+ electrodes) with tape to hold the Calibrant A in place. • Insert the K+ and Li+ electrodes into a sealed bag. Reagent Pack • Remove the reagent pack from the analyzer and discard. Analyzer Tubing • Remove all fluidic tubing and thoroughly rinse with DI water. ISE Module re-activation • Remove all electrodes from sealed bags. • Remove tape from K+ and Li+ electrode. • If necessary, soak the reference electrode in warm water until the lumen of the electrode has been cleared of salt build-up. • Install electrodes into the ISE Module. • Connect new reagent pack to the ISE Module. • Use Prime Cycles to prime the calibrants. • Calibrate.


13.3.11. Manual commands From Maintenanc e > Se rvic e > Manual > ISE

COMMAND BUTTON ISE? Calibration Sample Urine One Urine Two Clean Pump Calibration Start Purge / Position A Purge / Position B

DESCRIPTION Verify ISE Module communication. 2 point calibration. Empties flow-path for Serum Sample cycle Empties flow-path with Calibrant B washouts for Urine Sample Cycl Empties flow-path without Calibrant B washouts for Urine Sample Cycle Empties flow-path for Cleaning Cycle Empties flow-path for Pump Calibration Cycle Start Sample, Urine, Clean or Pump Cal Empties flow-path, dispenses100 μL Calibrant A and positions it in front of electrodes Empties flow-path, dispenses 100 μL Calibrant B and positions it in front of electrodes 146


Bubble Calibration Show Bubble Calibration Show Pump Calibration Pump Calibration A Pump Calibration B Version

Bubble Calibration Show last bubble calibration values Show last pump calibration values Pump xxx uL of Calibrant A into Sample Inlet Port. Pump xxx uL of Calibrant B into Sample Inlet Port. Request to show ISE software checksum version

Read mV Read mV of all four channels Show Last Slopes Show last calibration slopes Debug mV On Set debug mV ON One Debug mV On Set debug mode mV ON – No mV in Sipping Two Cycle Debug mV OFF Set debug mV OFF Maintenance Empties flow-path and NO Sipping Cycle Prime Calibrant A Empties flow path, dispenses 300 μL Cal A and positions it in front of electrodes Prime Calibrant B Empties flow path, dispenses 300 μL Cal B and positions it in front of electrodes


13.3.12. Operation commands From Maintenanc e > Operation > ISE

COMMAND BUTTON Start Up Bubble Calibration Pump Calibration Electrodes Cleaning Electrodes Calibration Change Reagent Pack

DESCRIPTION Verify ISE module communication, read and update information Pack and perform a 2 point calibration. Bubble Calibration Empties flow-path for Pump Calibration Cycle Perform a Maintenance command, 2 point calibration Perform a Maintenance command, ask for replace pack and after that read and update information Pack, Perform prime 148


Change Electrodes Maintenance Purge / Position A Purge / Position B

calibration A and B command. Perform a Maintenance command, ask for replace electrodes and after that Purge / Position A and B command, then 2 point calibration. Empties flow-path and NO Sipping Cycle Empties flow-path, dispenses100 μL Calibrant A and positions it in front of electrodes Empties flow-path, dispenses 100 μL Calibrant B and positions it in front of electrodes


13.3.13. Installation Kit ISE Medica (PN:KITISE40CMED) The kit is composed of the ISE module and his bracket (A) and the ISE pack compartment and peristaltic pumps (B).


1. Fix the module A with the screws (1). 2. Fix the module B with screws (5). 3. Pass the waste tubing through soft plastic hole (7) and connect in point (4). 151 . Pictus 700 Service Manual Rev.3

4. Pass calibrant A and B tubing through soft plastic hole (7& 6) and connect in point (3). For more information see interconnection diagram/tubing. 5. If the instrument was installedthe ISE module JS, remove connections (1) (2) (3) that are show in the diagram block. Regarding the connector (3) you need to change it with 2 terminal spade Code: VE0000A9 that is provide it in the KIT.

6. Depending on the SN you could need to make a hole tangent to the existing one for the ISE in the desk to align the Medica module. Use a drill tool to make a bigger hole of Ø17mm.

7. Put the aesthetic white ring M400CR02 over the hole. 8. Loose screws (2) then align tip arm and hole of the desk with the ISE module cup moving manually the back arm. 9. Install the PC rayo versión 1.9 or higher. Maintenance > Service > Parameters > Instrumental > ISE and Press ISE

10. Go to

Medica button to set the new module.

11. Set Medica parameters (For more information see section MEDICA Operational “

Parameters”).


12. Perform a mechanical calibración of back arm & ISE (For more Information see section mechanical calibration)

13.3.14. Troubleshooting 153 . Pictus 700 Service Manual Rev.3

13.3.14.1.Overview To enhance trouble-free operation of the ISE Module, it is important to follow the recommended component replacement schedule listed in the maintenance section of this manual. When the ISE Module is not operating properly, approach troubleshooting as a logical sequence of events. Isolate the problem area to avoid unnecessary component replacement and down time. Troubleshooting can be categorized into three main areas. These areas are: fluid delivery, electrode stability, and communication. Troubleshooting should focus primarily on fluid delivery and electrode have different causes. stability. As these are related, sometimes the same symptoms can All problems can be corrected while the ISE Module is still installed in the analyzer.

13.3.14.2.Communication Errors System Does Not Respond 1. Make sure that power is reaching the ISE Module. With the power turned on, you should be able to read 24VDC between T1 and T2 on the Interface Cable Assembly. You should also be able to read 24VDC between pins 27 and 29, or 28 and 30 on the main connector where the Interface Cable Assembly is connected to the ISE Module main PCD. 2. Turn off power to the ISE Module and re-apply the power. 3. Check the RS232 cable for damage. Replace the Interface Cable Assembly, if necessary.

13.3.14.3.Fluid Delivery The instrument performs a Pump Calibration cycle every time that the ISE module is initialized, after opening the software. This cycle will calibrate the pumps that dispense Cal A and Cal B, and position fluid in front of the electrodes. The waste pump moves solution from the Sample Entry port to the electrode area for measurement. As the tubing ages and samples are passed through the system, the positioning of the solution will change. The pump uses a stepper motor that counts how many steps it takes to move the solution to the correct position. By calibrating the pump each day, the ISE Module can now calculate the relationship between volume and pump steps. This will enable the pumps to dispense an accurate volume of calibrant, and for the solution to be accurately placed in the proper location for analysis. Problems can be caused by a partial obstruction from a clot in the tubing from the exit tube to the waste pump, a sharp bend in the waste tubing that restricts the flow, or a misalignment of the electrodes. As the pump tries to pull the fluid from the sample entry port intothe thetrailing electrodes, develops because of theetc.) restriction. Thethe bubble detects edge aofvacuum the solution (sample, calibrant, and stops wastedetector pump so that the solution is in front of the electrodes. The vacuum will cause the solution to travel after the pump stops. 154 . Pictus 700 Service Manual Rev.3

One of the first indications of a flow problem will be the lithium electrode response. If, however, the solution slowly moves out of the electrodes when the pump has not been activated, there is either a leak between the electrodes, or along the flow path. This can be tested by placing solution into the sample entry port by hand and watch to see if the fluid level changes. In either case, the symptoms would be similar —the lithium or sodium millivolts would experience noise errors and the bubble detector would trigger an error. NG

13.3.14.4.Electrode Stability Errors associated with electrode instability typically include drift, noise, and slope failures. While these errors may be caused by a failure of a particular electrode, it is necessary to explore other causes as well. Proper operation on a daily basis is the key to keeping the electrodes stable and the system working properly. Each day, it is necessary to perform a Cleaning Cycle. The cleaning solution removes protein build-up in the flow paths of both the electrodes and the tubing. In high sample volume instances, it may be necessary to perform this cycle more than once in a single day. The instrument will automatically require the cleaning solution after every sample reading batch. It is also necessary to replace the Reference Electrode every six months or when the red ball indicator no longer floats in the internal electrode solution, whichever comes first. Failure to replace the Reference Electrode at this interval can cause all three of the errors mentioned.

13.3.14.5.Troubleshooting Guide Symptom

Problem

Correction

1. No power.

System does not respond

Low Slope Na+ or K+<52mV/decade Cl<40mV/decade, Li+<47mV/decade or High Slope Na+,K+, or Li+>64mV/decade Cl>55mV/decade

2. Communication failure.

Turn off power, reapply power.

3. RS232 cable is disconnected or damaged.

Reconnect or replace cable.

4. ISE Module connector has been damaged.

Replace board.

5. Component failure on board.

Replace board.

1. Misalignment of electrodes.

Remove electrodes. Inspect o-rings. Reassemble properly.

2. Calibrator solutions.

Replace Reagent Pack.

3. Electrode (low slope).

Replace electrodes.

Remove electrode, tap to 4. Air bubble on reference electrode dislodge bubble, replace, membrane. and recalibrate. 5. Reference electrode.

Replace reference electrode and retest.

6. ISE Module or fluid temperatures

Change ISE Module 155


exceed 32° C. (high slope).

location if ambient temperature is too great.

1. Electrode.

Noise Error Flag Single electrode

2. Electrical noise spike from environmental source. 3. Component failure on ISE Module

Replace problem electrode and recalibrate. Find source of spike and eliminate. Check instrument grounding. Replace board.

board. 1. Reference electrode.

Noise Error Flag Multiple electrodes

2. Electrical noise spike from environmental source.

Replace reference electrode and recalibrate. Check for electrical noise coincident with activation. Check instrument grounding.

3. Component failure on ISE Module Replace board. board.

Drift Error Flag Single electrode

Purge the Calibrant A and recalibrate the ISE Module. 1. May occur when new electrode or If the electrode is new it Calibrant A is installed. may initially drift as it rehydrates over the course of 15 minutes. 2. Electrode.

Replace the electrode and recalibrate.

1. May occur when new electrode or Purge Calibrant A and Reagent Pack is installed on system. recalibrate. 2. Reference electrode. Drift Error Flag Multiple electrodes

3. Electrical spike from environmental source.

Replace reference electrode and recalibrate. Find source of spike and eliminate. Check instrument grounding.

4. Component failure on ISE Module Replace the board. board. 1. Insufficient sample pipetted into Air in Sample

Instrument must deliver 70 μL. Increase

ISE Module sample entry port.

dispensed sample volume.

2. Bubbles in Sample

Instrument must deliver sample free of bubbles. 156


Check probe tubings. 3. Fluid leaks

4. Sample not positioned

Determine source of leak and resolve. Electrodes not seated properly. Remove electrodes. Inspect orings and reassemble. Replace pump tubing.

5. Pump tubing obstructed.

Replace pump tubing.

1. Sample and Calibrant A are segmented with air.

Electrodes are not properly seated or compressed. Check compression plate, spring, and seal. Remove and reassemble electrodes. Use Manual Movements>Cleaning>IS E Module cleaning

Air in Sample and Calibrant A

2. Fibrin or salt is plugging the electrode flow path.

Remove electrodes and clean or replace electrode with plugged flow path. Reinstall electrodes and recalibrate.

3. Bubble detector is malfunctioning. Replace bubble detector. Check electrical connections. 4. Waste pump is malfunctioning.

Replace pump tubing. Replace motor. Replace pump. Electrodes are not properly seated. Check compression plate, spring and seal.

Air in Calibrant B and Air in Calibrant A

1. Calibrant B and Calibrant A are segmented with air.

Ensure that all electrodes and o-rings are properly installed. Ensure tubing between reagent pack and sample entry port is connected properly. Replace tubing between reagent pack and sample 157


entry port. Reagent low or out. Use Manual Movements>Cleaning>IS E Module cleaning 2. Fibrin or salt is plugging the electrode flow path.

Remove electrodes and clean or replace electrode with plugged flow path. Reinstall electrodes and recalibrate. Check electrical connections.

3. Waste pump malfunction.

Replace pump tubing. Replace motor. Replace pump.

Air in Calibrant A

4. Bubble Detector malfunction.

Replace bubble detector.

1. Calibrant A.

Replace reagent pack with a new one, prime, and recalibrate.

2. Tubing from reagent module is disconnected, plugged, or crimped.

Reconnect or replace tubing. Check electrical connections.

3. Calibrant A pump is not working properly.

Replace pump tubing. Replace motor. Replace pump.

Air in Segment

Calibration pump don’t work properly Calibrate vertical position due air bubbles when the probe ISE cup. dispense liquid. Air bubbles in the tip.

Check probe tubings.

G


14. Electronics 14.1. Maintenance 14.1.1. Special replacement procedure for Head board (M400- P299 or M400-P317) 1. Remove board plastic cover. 2. Disconnect both hydraulic and electrical connections to the board. 3. Set JP1 and JP2 jumpers according to the position of the board: Position Front arm Back arm

Jumper 1 Closed Open

Jumper 2 Closed Open

4. For instruments with M400-P317 board please perform


Adjust and verify probe optical collision sensor (M400-P317 Head board (7.2.5) 5. Reconnect and reinstall plastic cover.

14.1.2. SM, FW, and Temperature controller board replacement 1. Remove instrument left side panel. 2. Remove the containment bar unscrewing the 2 screws.

3. Remove the board you want to replace.

4. Place the new board. 160 . Pictus 700 Service Manual Rev.3

5. Place the containment bar and close the equipment.

14.1.3. CPU board replacement 1. Saved in the CPU memory, all the calibration informationregarding the instrument is stored. Before replacing the CPU, you will need to safeguard this information. a. If the old CPU is communicating with the PC and the memory is not damaged, from Maintena nce > Service > Instrument Mem ory . Push “Backup Memory”. Store the file in a safety place. b. Or, from the computer you were using the equipment, store the MemoryBackup.ini file located inside BIN folder in a safety place. c. Or, from the installation CD. 2. Replace the CPU following SM, FW, and Temperature controller board replacement (14.1.2) 3. From Maintena nce > Service > I nstrum ent Memory , push “Upgrade memory” and select the stored memory file. 4. If the MemoryBackup you usedis too old, once the memory was restored, follow procedure (4) in order to check the mechanical calibration. Perform a washer volume calibration fromMaintena nce > Washer Volu me Calibration . Follow Adjustment of filter delays (6.2.2). Perform a full photometer calibration from Maintena nce > Photom ete r Calibra tion.

14.1.4. Procedure to replace PL400242W stepper motor controller board for PL400313W microstepper motor controller board

PL400313W


PL400242W 1. The instrument has 6 motor controller boards to drive movements (PL400242W or PL400313W). 2. You can replace a PL400242W for PL400313W. Note: You can not replace a PL400313W for PL400242W in instruments with SN:xxxx41xx. Follow procedure SM, FW, and Temperature controller board replacement (14.1.2) 3. After replace the board you have to perform the mechanical calibration of the movement that this board controls. Important: - To use an instrument with PL400313W board you must run a rayo software version 1.7.3

or higher. - Take note that if you replace only one board (PL400313W), for example the front vertical and horizontal arm movement, both arms will move different. The front arm will has smoother movement and less mechanical noise.

14.1.5. Removal of preamplifier 1. Perform procedure Complete removal of reaction tray (8.2.1) . 2. Remove the preamplifier screw.


15. Housing 15.1. Maintenance 15.1.1. Instrument cover installation procedure 1. Remove right lateral and rear panel. 2. Install the instrument cover in the position and place the hinge axis, do not adjust the set screw in this step.

Clot

3. Install the gas spring.

4. Check the adjustment of theinstrument cover over the deck. If there isn´t collisions adjust the set screw.

5. If you detect side collisions, between the instrument cover and deck´s borders turn the


tensor screw as the schema to move the instrument cover to the right position.

6. Adjust the rear hinge´s set screws, put the lock cup to the gas spring, and place the lateral and rear panel.


16. Charts and diagrams


16.1. Location of belts and sensors Reference beamsplitter

Filter wheel

Front/Back beamsplitter Lamp

Place for back reaction tray cuvette Place for front reaction tray cuvette Home sensor

Photometer assembly

– top

view

Horizontal Belts (2)

Horizontal Home

Vertical Belt

Horizontal Verif sensor

Vertical verif sensor Vertical home sensor

Robot assembly overview

– side view


Washer Belt

Home sensor

Washer assembly

– side view

Home and verif sensor

Sample tray belt

Reag ent tray Belt Home and verif sensor

Sample and rea

gent tray assemb

ly – bottom v

iew


Home and verif sensor

Reaction tray belt

Rea ction tray – bottom v

ie w


16.2. ISE Hydraulics schematic.


17. General parameters Notes * Parameters cannot be modified when instrument is in use. * Wear parameters refers to a factory recommendation for warning on consumables expected life. Each parameter, when surpassed, triggers a warning message. The warning message presence does not prevent from instrument usage. * For the cuvette washer, water is delivered in four cuvettes at the same time, between 500 and 700 microliters in each one. So, total volume is between 2000 and 2800 microliters. Calibration is in step of peristaltic pumps.

NAME

BLOCK

TYPICAL VALUE

COMMENTS

ACCESS LEVEL

PATH

Low limit (Abs)

Cuvette Blank

0

Minimum absorbance for a valid cuvette

User

Maintenance\Parameters\Use

High limit (Abs)

Cuvette Blank

0,2

Maximum absorbance for a valid cuvette

User


Tolerance

Cuvette Blank

Primary area (mm2 x 10)

0,04

Tolerance to detect a dirty cuvette after washing

User


Sample vials

950

Area for the default vial

User


Secondary area (mm2 x 10) Sample vials

950

Area for the secondary or pediaric vial

User


Filter 1 (Lambda)

Photometer

340

Wavelenght for the specified filter

Service

Maintenance\Service\Parameters\Instrumental\Filters

Filter 2 (Lambda)

Photometer

380


Service


Filter 3 (Lambda)

Photometer

405


Service


Filter 4 (Lambda)

Photometer

450


Service


Filter 5 (Lambda)

Photometer

490


Service


Filter 6 (Lambda)

Photometer

505


Service


Filter 7 (Lambda)

Photometer

550


Service


Filter 8 (Lambda)

Photometer

590


Service


Filter 9 (Lambda)

Photometer

620


Service


Filter 10 (Lambda) Filter 11 (Lambda)

Photometer Photometer

650 700

Wavelenght for the specified filter Wavelenght for the specified filter

Service Service

Maintenance\Service\Parameters\Instrumental\Filters Maintenance\Service\Parameters\Instrumental\Filters

Filter 12 (Lambda)

Photometer

750


Service


Filter 13 (Lambda)

Photometer

0


Service


Front Arm

Temperatures

41

Preheater temperature

Service

Back Arm

Temperatures

41

Preheater temperature

Service

Maintenance\Service\Parameters\Instrumental\Others

Reaction Tray

Temperatures

39

Incubation chamber temperature

Service


Cool Tray (H/L)

Temperatures

Low limit (Abs)

Cuvette Blank

0

High limit (Abs)

Cuvette Blank

0,2

8 (H) / 7 (L) Temperature control range for the reagent cooler


Service


Minimum absorbance for a valid cuvette

Service


Maximum absorbance for a valid cuvette

Service


Tolerance (Abs)

Cuvette Blank

0,04

Tolerance to detect a dirty cuvette after washing

Service


Pinch valve time

ISE

500

Time to activate the pinchvalve to fill the ISE

Service


Serum

ISE Thresholds

1700

Threshold to detect serum

Service


Urine

ISE Thresholds

3000

Threshold to detect urine

Service


Std A

ISE Thresholds

1500


Service


Std B

ISE Thresholds

2500


Service


Time int ext wash (ms)

Pumps

750

Time to wash the probe before a new dilution

Service


Time sys flush (ms)

Pumps

4750

Time the pump is on for a system flush

Service


Decompression (ms)

Pumps

250

Time required to decompress the pump

Service


Pump turns

Wear

50000

Maximum allowed number of cycles

Service


Syringe cycles

Wear

100000


Service


Dryer block cycles

Wear

20000


Service


ISE number of samples

Wear

10000


Service


Front volume (Steps)

Washing Station

1500

Number of steps to fill the cuvette during washing

Service

Back volume (Steps)

Washing Station

1500

Number of steps to fill the cuvette during washing

Service


Front time down (ms)

Washing Station

1300

Time to keep the wash head down during the drying cycle

Service


Back time down (ms)

Washing Station

1300

Time to keep the wash head down during the drying cycle

Service


Volume

Pre & Post Wash

100

Volume for pre and post washes

Service




Diluter speed

Pre & Post Wash

4320

Syringe speed during the pre and post washes

Service



18. Complementary information 18.1. Identification of system leds Location

Led silkscreen/color

On each power supply

Green led

Power on push button

Red lamp

Backplane board PL400245

+36_1/Red +36_2/Red +36_3/Red +36_4/Red +12/Green

Description Power good signal from power supply Blinks on a power supply fault event 36v on J21* 36v on J20* 36v on J60* 36v on J57* 12v on J72*

Bar code reader interfase board PL230224

DL1/Green DL2/Red

Read Ok Read order / init to BCR

RS232 to RS232 Isolated board PL400282

+5vPC/Red +5vCPU/Red

Power from standby power supply (allways on) Power from CPU board (on when instrument is on)

Microconverter baseplane & Power supply board PL400248

DL1/Green DL2/Red DL3/Red

+12 input +12A output -12A output

Power ON/OFF control board PL400255

+12v cooler led/Red +12v lamp led/Red +36v heater led/Red +36v Motor II led/Red +36v Motor I led/Red +12v Logic led/Red +24v Diluters led/Red Cooler activated led – Solid state relay/Red Mains activated led – Solid state relay/Red

Power good from power supply Power good from power supply Power good from power supply Power good from power supply Power good from power supply Power good from power supply Power good from power supply JP1: cooler pushbutton bypass JP2: mains pushbutton bypass

ISE protocol interfase board PL400268

DL1/Red DL2/Red DL3/Red DL4/Red

+12 Ise busy RS485 Cavro Blink (development purposes) Trigger 1 (development purposes)


Cooler control board PL400269

DL1/Red DL2/Red DL3/Green

Power on J12/J13: Peltiers Power on J14/J15: Peltiers +12

Lamp Control board PL400273

DL1/Red

Lamp on signal


Front tip pump Back tip pump Not in use: Front Water pump Front Drier pump Front Reagent pump Back Reagent pump Back Drier pump +12

Pumps distribution board PL400283


Notes: * Remove all motor controller boards to perform led checkings (any motor controller internally wires J21 to J20 and J60 to J57 depending its position on backplane). •

Dual Stepper motor controller PL400242 status leds: Green led Red 1 led

Red 2 led Verification signal Collision(wa sher only) •

Normal Mode

•

On

•

Home signal

•

•

Filter wheel motor controller status leds: Green led Red 1 led

Normal Mode Busy Verification problem No home detection

• • • •

On Off Blinking Blinking

• • • •

Home signal On Off On

Red 2 led • • • •

Filter signal On Off Off

To obtain the program folder press the right button on the autoanalyzer icon and choose properties.

18.2. Computer program folder contents v1.7.3 or lower

.Root Backup

Autoanalyzer Program folder and Rayo .exe file Backup of Data, Historic, Translator and Use data folders

Bin

Software parameters, wears, memory backups and about.txt


Data Historic Cumulative Historic Interfase Lims NSI data Priv Priv thread Reports Translator Use data

Configurations, methods, caligrations, etc. All historical tables. Historic files separated by year and month. Tables shown on screen Information for laboratory information management system Calibration reports and summaries, communication logging, etc. Used by the database engine Used by the database engine Tables containing information to build reports Information for language support Samples, patients, tests, tray definition.

v1.8.0 or higher

.Root Bin Database translator, etc. Cumulative Historic System Test

Autoanalyzer Program folder and Rayo .exe file Software parameters, wears, memory backups and about.txt Database files including methods, calibrations, historic, Historic database separated by year and month. All the system tests ran in that computer.

18.3. Main menu user messages MESSAGE On line

OPERATION Instrument is ready to process commands.

Operating Connecting Offline

Instrument is busy. Computer trying to set the link to the autoanalyzer. Computer was not able to connect to the instrument. Instrument was unable to recover from an error - A fatal error occurred. User attention required. Washer is disabled and no empty cuvettes available. Awaiting confirmation of result by the user. Awaiting confirmation of calculated result by the user.

System log

System Alerts Reaction tray Full Reaction pending Acc. Calculated pending Acc. Reagent blank pending Awaiting confirmation of measured blank by the user. Acc. Instrument performing hydraulic priming and reactiontray Pre Automatic heater warm up. Instrument processing samples (cuvette checking, reagent Automatic level checking, processing of blanks, standards, controls and samples). Instrument washing used cuvettes and cleaning the probes Post-Automatic with wash and soak solutions



19. APPENDIX 19.1. Glossary

Absorbance. Absorbance, or optical density, is a measure of the amount of light absorbed by a solution. Absorbance is equal to the logarithm of the ratio of incident light to transmitted light. Acceptance. The action of approving or rejecting the result of an assay. Used by the operator to confirm calibrations or out of range assays results. Accuracy. The closeness of the result of a measurement to the true value. Aliquot. A measured portion of a sample taken for analysis. Analyte. A specific compound or element of interest undergoing chemical analysis. Batch. A quantity of material produced or processed in one operation, considered to be a uniform discrete unit. Batch-sample. One of the samples drawn from a batch. Batch-size. The number of samples in a batchlot. Bichromatic measurement. The substraction of a secondary wavelenght absorbance reading from a primary wavelenght absorbance reading to obtain a delta absorbance reading. BRTW. Back reaction tray and washer. BVH. Back vertical & horizontal. Calibrate. To determine, by measurement or comparison with a standard, the correct value of each scale reading on a meter or other device, or the correct value for each setting of a control knob. Calibration curve. The graphical relationship between the known values for a series of calibration standards and instrument responses. Calibration drift. the difference between the instrument response and a reference value after a period of time without recalibration. Calibration standard. A substance or reference material used to calibrate an instrument. Certified Reference Material. A material that has been certified for accuracy, stability and physical form. Coefficient of variation (CV). A measure of relative dispersion (see precision). It is equal to the ratio of the standard deviation divided by the arithmetic mean. Concentration. The concentration of an analyte is a measurement of the amount of mass of the analyte per unit volume or weight. The mass may be expressed in grams, moles, international units (IU), or other units. The liquid volume may be expressed in milliliters or other units of volume. Sometimes only the total mass of the analyte is used. Control limits. A range within which specified measurement results must fall to be compliant. Control Specimen. A control specimen is material from a single pool of unknown specimen(s) from which an aliquot is measured every analyte run to monitor assay to assay reproducibility and to provide an indirect measurement of the performance of the assay components. Correlation coefficient. A number between -1 and 1 that indicates the degree of linearity between two variables or sets of numbers. The closer to -1 or +1, the stronger the linear relationship between the two (i.e., the better the correlation.) Values close to 176 . Pictus 700 Service Manual Rev.3

zero suggest no correlation between the two variables. Dilution factor. Indicate the ratio between the srcinal concentration and the new dilution concentration. Used when analyte concentration is too high and sample have to be dilutedghin order to perform the assay. A typical dilution of 1 part of sample in 10 parts of final solution, is commonly represented as 1:10 (1in 10) or 1+9 (1 plus 9). Duplicate. A second aliquot of a sample that is treated the same as the srcinal sample in order to corroborate the obtained result. Eeprom. Electrically erasable and programmable read only memory. End Point Measurements. End point measurements are measurements of a reaction, which is made at a fixed time, usually after the reaction has been completed. Error Message. An error message is a warning which is displayed whenever an error occurs during the operation of the program. A record of the type of error, its location, and its cause is stored in the Errors.Log text file. External quality control. The activities which are routinely initiated and performed by persons outside of normal operations to assess the capability and performance of a measurement process. FBPP. Front & back peristaltic pumps. FRTW. Front reaction tray and washer. FVH. Front vertical & horizontal. FW. Filter Wheel. Good laboratory practices (GLP). Either general guidelines or formal regulations for performing basic laboratory operations or activities that are known or believed to influence the quality and integrity of the results. Goodness-of-fit. the measure of agreement between the data in a data set and the expected or hypothesized values. Interference. a positive or negative effect on a measurement caused by a variable other than the one being investigated. IR Infrared. Kinetics Analysis. A kinetics analysis measures the change in concentration of the starting and end products over time. Usually applied to enzymatic reactions. Laboratory control standard.A standard, usually certified by an outside agency, used to measure the bias in a procedure. Lambert-Beer Law. The Lambert-Beer Law is an equation which describes the linear relationship between the absorption and the concentration of the absorbing material (absorber). The law states that absorbance (ABS) is equal to absorptivitya, multiplied by the pathlength or the distance the light travels through the sample b, multiplied by the concentrationc (ABS= abc). Linearity. In kinetics determinations, the degree of agreement between the measured points and a straight line assumption. Mixer. Robotic component that agitates the probe on the cuvette. Pool (biological). A combination of biological specimens (i.e., urine or serum) from many workers that is used to prepare small aliquots to be run with each batch of analyses. Aliquots of these pools are analyzed with each batch of samples and the data are used to develop quality control charts. Precision. The repeatability or reproducibility of individual measurements expressed as Coefficient of variation (CV). Preventative maintenance. An orderly program of activities designed to ensure against equipment failure. Proficiency testing. Any inter-laboratory testing program where stable specimens are sent to participating laboratories for analysis. Results from all participating 177 . Pictus 700 Service Manual Rev.3

laboratories are compared, pooled, and tabulated by the testing program operator with the purpose of improving laboratory performance. Quality assurance program. Written policies and procedures that outline how the laboratory intends to produce data of known and accepted quality controls. Quality Control (QC). The operational techniques and activities that are used to fulfil requirements for quality. Reagent blank Reagent(s), without analyte or sample added, which are analyzed to determine their contribution to the total absorbance reading. Reliability. The likelihood that an instrument or device will function under defined conditions for a specified period of time. SRT. Sample & Reagent Tray. Standard solution. A solution containing a known concentration of analytes, prepared and verified by a prescribed method or procedure and used routinely in an analytical method. Standard. Standard samples are aliquots of calibration specimens containing predetermined quantities of the analyte. The response of each standard, along with the standard's predetermined concentration, is used to construct a standard calibration curve. From this standard curve, sample concentrations can be computedusing the response from the sample. Stat. Assay requiring immediate ressults. TC. Temperature controller. Test result. A product obtained from performing a test or assay determination. UV. Ultraviolet.


19.2. Technical specifications Throughput: 450 tests/hour double reagent, 650 tests/tour mono reagent, max. 770 tests/hour with optional ISE unit. Analysis Modes: End point with sample or reagent blank. Factor or standard.

Reagents: Maximum number of simultaneous tests: 36 double to 72 single reagent tests + 3 with optional ISE unit. 1 to 3 reagents, 5 to 500 µL/test each (in increments of 1 µL), final total solution volume 180 to 500 µL/test Reagent bottles capacities: 25, 40 and 70.

Priority selection by sample (profile) or by reagent (batch). Calibration curve with up to 10 standards. Automatic curve fit. Turbidimetry. Fast and two-point kinetics (zero and first order). Routine, batch, STAT procedures, profiles. Enzymes. Drugs. Automatic sample dilution on abnormal levels, excessive substrate consumption and/ or lack of linearity. Full quality control: Levy-Jennings plots, Westgard multirules. Import/export data, methods and historic files. Automatic backup procedure. Test selection, automatic calibration, calibration curve, multipoint calibration,

Reagent cooling compartment: 72 cooled positions.

polygonal. Serum indices: sample blank compensation, calculated tests, Quality control, auto re-run, prozone check, record of calibration, data storage (historic results). Automatic pre-dilution and post-dilution (ratio 1:5 to 1:100) Stat: Highest priority in operation. Continuous sample load

ISE Unit: Na+, K+ and Cl- measurements. Samples: serum or urine. Other electrodes on request.

Sampling and reagent Samples: Sample volume: 2 to 100 µL/te st (in increments of 0.2 µL. Sample Tray: 95 (5 racks x 19 positions) ID bar code equipped positions for routine, stat and control samples and standard solutions. Primary tube (length up to 100 mm), Pediatric vial

Reaction: Water consumption: 3 L / hour. Warm air incubator: room, 30ºC and 37ºC. Reaction cuvette: re-usable plastic 6 mm path light cuvettes Reaction time: 0 to 10 min. Reaction temperature: 3 7ºC ± 0.1ºC. Stirring: After dispensing each reagent. Optic System : Double beam Photometric Range: -0.1 to 3.6 A. Measuring wavelength: 340 to 800 nm (selectable among 12 wavelengths). Photometry: Single or Double-wavelength simultaneous reading.

Data Management: Windows TM based Software. Interface LIS: bi-directional RC 232 C, according to ASTM 1394 requirements. Printout: Customer’s optimized (Analysis result, work list, serums list, Quality Control, Calibration curves, etc.) Power Requirements: 110/220V, 50/60 Hz, 2.0 kVA Dimensions: 97(w) x 67(D) x 100 (H) cm. Weight: 115 Kg.


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P700 Service Manual

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