BRITISH STANDARD
Essential oils — Determination of relative density at 20 ºC — Reference method
ICS 71.100.60
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BS ISO 279:1998
BS ISO 279:1998
National foreword This British Standard reproduces verbatim ISO 279:1998 and implements it as the UK national standard. The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential oils, which has the responsibility to: —
aid enquirers to understand the text;
—
present to the responsible international/European committee any enquiries on the interpretation, or proposals f or change, and keep the UK interests informed;
—
monitor related international and European developments a nd promulgate them in the UK.
A list of organizations represented on this committee can be obtained on request to its secretary. Cross-references The British Standards which implement international publications referred to in this document may be found in the BSI Standards Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Find” facility of the BSI Standards Electronic Catalogue. A British Standard does not purport to include all th e necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations.
This British Standard, having been prepared under the direction of the Consumer Products and Services Sector Committee, was published under the authority of the Standards Committee and comes into effect on 15 May May 2001 2001
Summary of pages This document comprises a front cover, an inside front cover, the ISO title page, page ii, pages 1 to 4, an inside back cover and a back cover. The BSI copyright date displayed in this document indicates when the document was last issued.
Amendments issued since publication publication Amd. No. © BSI 05-2001
ISBN 0 580 37439 4
Date
Comments
INTERNATIONAL STANDARD
ISO 279 Second edition 1998-12-15
Essential oils — Determination of relative density at 20 °C — Reference method Huiles essentielles — Détermination de la densité relative à 20 °C — Méthode de référence
A
Reference number ISO 279:1998(E)
ISO 279:1988(E)
Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. International Standard ISO 279 was prepared by Technical Committee ISO/TC 54, Essential oils . This second edition cancels and replaces the first edition (ISO 279:1981), which has been technically revised.
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INTERNATIONAL STANDARD
ISO 279:1998(E)
Essential oils — Determination of relative density at 20 °C — Reference method
1 Scope This International Standard specifies the reference method for the determination of the relative density of essential oils at 20 °C. NOTE If it is necessary to perform the test at a different temperature on account of the nature of the essential oil, the temperature should be mentioned in the International Standard appropriate to the essential oil concerned. The average correction in the region of 20 °C is from 0,000 7 to 0,000 8 per degree Celsius.
2 Normative reference This following normative document contains provisions which, through reference in this text, constitute provisions of this International Standard. For dated references, subseqent amendments to, or revisions of, this publication do not apply. However, parties to agreements based on this International Standard are encouraged to investigate the possibility of applying the most recent edition of the normative document indicated below. For undated references, the latest edition of the normative document referred to applies. Members of IEC and ISO editions maintain registers of currently valid International Standards. ISO 356, Essential oils — Preparation of test samples.
3 Terms and definitions For the purposes of this International Standard, the following terms and definitions apply. 3.1 relative density at 20 °C ratio of the mass of a given volume of the oil at 20 °C to the mass of an equal volume of distilled water at 20 °C NOTE
20
This quantity is dimensionless and its symbol is d 20 .
3.2 absolute density at 20 °C of an essential oil ratio of the mass of a given volume of the oil at 20 °C to the same volume NOTE
This quantity is expressed in grams per millilitre.
4 Principle Equal volumes of the essential oil and water, at 20 °C, are weighed successively in a pyknometer.
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ISO 279:1998(E)
5 Reagents 5.1 Distilled water water,, freshly boiled and subsequently cooled to approximately 20 °C.
6 Apparatus Ordinary laboratory apparatus and the following. 6.1 Glass pyknometer, pyknometer, of of minimum nominal capacity of 5 ml. NOTE 1 There are are available available on the market market automatic automatic electronic electronic instrumen instruments ts for the accurate accurate measuremen measurementt of the relative relative density. Such instruments may be used for routine controls, but in cases of dispute the reference method is the pyknometric method. NOTE NOT E2
Approp App ropria riate te pykno pyknomet meters ers are are descr describe ibed d in ISO ISO 3507 3507 [2]. [2].
6.2 Water bath bath,, capable of being maintained at 20 °C ± 0,2 °C. 6.3 Standardized thermometer thermometer,, graduated from 10 °C to 30 °C, with 0,2 °C or 0,1 °C divisions. 6.4 Analytical balance balance,, accurate to 0,001 g. g.
7 Sampling It is important that the laboratory receive a representative sample which has not been damaged or modified during transportation or storage. Sampling is not part of the method specified in this International Standard. A recommended sampling method is given in ISO 212 [1].
8 Special Prepare the test sample in accordance with ISO 356.
9 Procedure 9.1 Preparation of pyknometer Carefully clean the pyknometer (6.1) and then rinse it successively with, for example, ethanol and acetone, then dry the interior by means of a current of dry air. If necessary, wipe the outside with a dry cloth or a filter paper. When temperature equilibrium is reached between the balance case and the pyknometer, weigh the latter with its stopper, if any, to the nearest 1 mg.
9.2 Weighing of distilled water Fill the pyknometer with distilled water (5.1). Immerse the pyknometer in the water bath (6.2). After 30 min, adjust the water to the mark, if necessary. Insert the stopper, if any, and dry the outside as before with a dry cloth or a filter paper. When temperature equilibrium is reached between the balance room and the pyknometer, weigh the latter and its stopper, if any, to the nearest 1 mg.
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ISO 279:1998(E)
9.3 Weighing of essential oil Empty the pyknometer, then wash it and dry it as specified in 9.1. Proceed as specified in 9.2, replacing the water by the test sample prepared according to clause 8.
10 Expression of results The relative density,
20 d 20 ,
is given by the following equation:
m2 - m0 m1 - m0
where m0
is the mass, in grams, of the empty pyknometer determined in 9.1;
m1
is the mass, in grams, of the pyknometer filled with water, determined according to 9.2;
m2
is the mass, in grams, of the pyknometer filled with the essential oil, determined according to 9.3.
Express the result to three decimal places. NOTE 1
In pract practice, ice, no corre correctio ction n is made made for for the upth upthrus rustt due to to air. air.
NOTE 2
Electronic instruments often register higher accuracy levels.
If the absolute density of the essential oil is required, multiply the value obtained for the relative density by the absolute density of water at 20 °C (i.e. 0,998 23 g/ml).
11 Test report The test report shall state:
the method used; the result obtained; and if repeatability has been verified, the final result obtained. It shall also mention any operating conditions not specified in this International Standard, or regarded as optional, as well as any circumstances that might have influenced the results. The test report shall include all details required for the complete identification of the sample.
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ISO 279:1998(E)
Bibliography
[1] ISO 212, Essential oils — Sampling. [2] ISO 3507, Laboratory 3507, Laboratory glassware — — Pyknometers.
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BS ISO 279:1998
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