Biotechnology Practice Exam 1

BIO 230

Take Home Exam 1 ___________________________________Name

Directions: Directions: You may use your your book, notes notes and text website website to help answer answer the questions. questions. You M AY NOT use  any ot her person person t o help you answer t he quest quest ions. ions. Please maintain your own personal and academic integrity. Thanks! Due Due 11:0 11:00 0 AM on 10/ 10/ 03/ 03/ 11  MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers answers the question.

1) Scientists at the biotechnology company Genentech created the first recombinant DNA product for use in humans. Approved by the Food and Drug Administration in 1982, this product was: A) A gene gene chip chip B) Human growth growth hormone hormone C) Chymo Chymosin sin D) Erythropoiet Erythropoietin in E) Insul Insulin in

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2) The use of computer hardware and software to analyze biological data such as DNA sequence comparison data is known as: A) Recombinant Recombinant DNA technology technology B) Genomic Genomicss C) Bioremed Bioremediati iation on D) Bioinform Bioinformatic aticss E) Nanotechno Nanotechnology logy

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3) Studying the entire complement of DNA in an organism's cells is known as: A) Genomic Genomicss B) Bioremed Bioremediati iation on C) Recombinant Recombinant DNA technology technology D) Bioinform Bioinformatic aticss E) Nanotechno Nanotechnology logy

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4) Combining DNA from different sources is an example of: A) Genomic Genomicss B) Nanotechno Nanotechnology logy C) Bioremediation D) Bioinformatics E) Recombinant DNA technology

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5) ________ is the use of living organisms or their products to clean up the environment, improve human health, and provide more nutritious and diseasedisease- free food. A) Biotechnology B) Bioprocessing C) Bioremediation D) Genetic Genetic engineerin engineering g E) Biodegrad Biodegradatio ation n

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6) Subtle differences in DNA sequences that vary from person to person are called: A) Genomic Genomicss B) Chromos Chromosome omess C) DNA microarrays microarrays D) Single Single- nucleotide polymorphisms E) Pharmacogen Pharmacogenomics omics

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7) The co complementary ba base th that hydrogen bonds wi with th thymine in in a DNA do double he helix is: A) Guanine B) Cytosine C) Uracil D) Adenine E) Thymine

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8) Wh Which of the following is a structural feature of DNA but not RNA? A) Contains Contains phosphate phosphate groups groups B) Single stranded stranded C) Contains Contains the nitrogenous nitrogenous base uracil D) Contains Contains the nitrogenous nitrogenous base adenine E) Contains Contains deoxyribose deoxyribose sugars

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9) Addition of a poly(A) tail to mRNA molecule: A) Allows the mRNA to be recognized by by ribosomes during translation B) Is an important important step in translation translation C) Results in removal of of introns D) Allows mRNA mRNA molecules to be more stable in the cytoplasm cytoplasm E) Only occurs in bacteria

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10) This enzyme separates strands of DNA during DNA replication to make DNA single stranded so it can be copied: A) DNA polymerase B) DNA helicase C) DNA primase D) Reverse transcriptase

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E) DNA DNA ligase ligase 11) Which organelles are the site of ATP production by aerobic cellular respiration? A) Mitocho Mitochondr ndria ia B) Riboso Ribosomes mes C) Golg Golgii D) Rough endoplasmi endoplasmicc reticulum reticulum E) Lysoso Lysosomes mes

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12) ________ bind to mRNA and tRNA during translation and allow for polypeptides to be synthesized. A) Chromos Chromosome omess B) Riboso Ribosomes mes C) Nucl Nuclei ei D) Chlo Chloropl roplasts asts E) RNA polymerases polymerases

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13) ________ is the enzyme that copies DNA during DNA replication. Thermostabile forms of this enzyme are essential for PCR. A) RN RNA A primase primase B) RNA polymera polymerase se C) DNA polymera polymerase se D) DNA DNA ligase ligase E) DNA helicase helicase

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14) Which of the following is the typical start codon in most mRNA molecules? A) CAG B) UGA C) AAA D) AUG

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E) GAA

15) Which ich typ type of of RN RNA mo molec lecules les transpo sport am amino ino aaccids ids to the ri ribosom omee du during ing tr transla slatio tion? A) rRNA B) miRNA C) tRNA D) mRNA E) siRNA

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16) Which type of RNA molecules contain the genetic code of a gene that is read by ribosomes during translation? A) siRNA B) mRNA C) miRNA D) tRNA E) rRNA

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17) Synthesis of RNA from a DNA template is called: A) Reverse transcription transcription B) Translat Translation ion C) Transfor Transformat mation ion D) Transcri Transcriptio ption n E) None of of these choices

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18) Which of the following techniques involves hybridizing a cDNA sample to a chip containing thousands of singlesingle- stranded DNA sequences, sequences, allowing one to study the expression of thousands of genes simultaneously? A) Agarose Agarose gel electrophores electrophoresis is B) FISH C) Southern blot D) PCR E) DNA microarray

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19) When making a complementary DNA (cDNA) library, which enzyme is used to copy mRNA into DNA? A) RNA polymerase B) DNA DNA ligase ligase C) DNA polymera polymerase se D) Primas Primasee E) Reverse transcriptase transcriptase

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20) In a recombinant DNA experiment, which enzyme is used to join to gether DNA fragments by forming phosphodiester bonds between nucleotides? Recall that this same enzyme joins together Okazaki fragments on the lagging strand during DNA replication. A) DNA helicase helicase B) DNA primase primase C) DNA polymera polymerase se D) Reverse transcriptase transcriptase E) DNA DNA ligase ligase

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21) During library screening, PCR, Southern blotting, and other techniques, binding two pieces of DNA to each other by hydrogen bonding is called: A) Hybrid Hybridiza ization tion B) Autorad Autoradiogr iograph aphy y C) Reverse transcription transcription D) DNA liga ligation tion E) Polyadenylatio Polyadenylation n

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22) ________ is a DNA binding dye that fluoresces when DNA in an agarose gel is illuminated with ultraviolet light. A) Ethidium Ethidium bromide bromide B) Lactic Lactic acid C) Casei Casein n D) Ethano Ethanoll E) Malt Malt

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single- stranded DNA molecule attached to a radioactive or fluorescent compound 23) A ________ is a singlethat is complementary to a specific sequence of DNA. Such pieces of DNA are used to identify and study cloned genes in hybridization experiments. A) Vector Vector B) Probe C) Promoter D) Polypeptide E) Plasmid

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24) Transformation Transformation in a cloning experimen experimentt is: A) Using PCR to clone a gene B) Ligating pieces of foreign DNA together C) A technique for determining gene copy number in a genome D) Inserting Inserting DNA into bacteria bacteria cells cells E) Cutting DNA DNA with restriction enzymes enzymes

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25) Which of the following is an incorrect statement about restriction enzymes? A) Restriction enzymes usually recognize palindromic sequences B) Restriction Restriction enzymes enzymes can cut to create blunt- ended pieces of DNA C) Restriction Restriction enzymes can cut to create overlapping overlapping single- stranded ends of DNA D) Restriction enzymes create phosphodiester bonds between pieces of DNA in a cloning experiment E) Most restriction restriction enzymes enzymes are isolated from bacteria bacteria

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26) Approximately how large is the human genome? A) 13 million million bp B) 20,00 20,0000 bp C) 1 billion billion bp D) 3 billion billion bp E) 30 billio billion n bp

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restriction enzymes and how 27) Based on what you know about which organisms naturally produce restriction restriction enzymes are named, which of the following enzymes is isolated from the bacterium Bacillus amyloliquefaciens? amyloliquefaciens? A) BamHI B) SmaI C) EcoRI D) PstI E) HindIII

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28) Ap A pproximately how many genes are present in the human genome? A) 50,000 50,000 genes genes B) 20 billion billion genes genes C) 100 100,000 ,000 genes genes D) 20,000 20,000 genes genes E) 3 billion genes

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29) Which of the following techniques is the best choice for amplifying DNA? A) Microarray Microarray analysis analysis B) DNA sequen sequencing cing C) Affinity chromatography chromatography D) PCR PCR E) Southern blot analysis analysis

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30) 30) Which Which of the follo following wing techni techniques ques is most most com commo monly nly used to separat separatee and and aanal nalyze yze DNA DNA by by size? size? A) DNA microarray microarray analysis analysis B) DNA libra libraries ries C) Agarose Agarose gel electrophores electrophoresis is D) PCR PCR E) Hybridization

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31) A ___ _____ ______ cons consis ists ts of clon cloneed DNA DNA fra fragme gments nts for for all expr xpresse essed d gen genes es in a part partic icul ulaar tis tissu sue. e. A) Guggenheim library B) shotgun library C) genomic DNA library D) cDNA library E) PCR library

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32) Which of the following techniques techniques is most efficient for transforming transforming bacterial bacterial cells? A) Gene gun B) Pronuclear microinjection C) Electroporation Electroporation D) Sper Sperm m- mediated transfer E) ES cell cell transfer transfer

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ESSAY. Write your answer answer in the space provided provided or on a separate sheet of paper. paper. Use complete sentences sentences please. example of a risk and associated hazard. 6 33) Please descibe the difference between a risk and a hazard. Give an example points.

34) Please describle the importance of being diligent with your laboratory notebook and what are the consequences of not following the proper procedure with respect to writing in your lab notebook. 6 points. entry for the procedures on making brownies. brownies. You 35) On a separate piece of paper, write a "mock" lab notebook entry must follow all all steps discussed in class. class. 10 points.

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