Bacteria Biochemical Tests

Biochemical Test for Microbial Identification Test/ reaction Catalase decomposition Oxidase oxidation Indole utilization

Substrate * 3% H2O2

Enzyme Catalase

End-product End-produc t H 2O

Indicator H2O2

Pos & Neg + gas  –  gas  gas

1% tetramethyl-p phenylenediamine  phenylenediamine dihydrochloride tryptophan

Cytochrome oxidase

indophenol

*

+ Dark purple - Nochange

tryptophanase tryptophana se

indole

Erlich’s

+ red - yellow +bl green -nochange + bright red - no change + red - orange +purple -yellow or pink + blue - green + blue - green + dark brown - light brown

+ Ether cinnamaldehyde

Urease hydrolysis PYR hydrolysis Hippurate Hydrolysis

urea

urease

ammonia

phenol red

L-pyrrolidonyl-Bnaphthylamide hippuric acid

L-pyrroglutamylaminopeptidase hippurase

Bnaphthylamimide glycine,  benzoic acid acid

 N,N,methylamino  N,N,methylamino cinnamaldehyde ninhydrin

Acetamide Utilzn,deamination Acetate Utilization# Bile Esculin hydrolysis

Acetamide

deaminase

ammonia

bromthymol blue

Sodium acetate

Source of carbon

Shift to alkaline

Bromthymol blue

40 %Bile-inhibtor %Bile-inhibtor esculin

hydrolase

esculetin

Ferric amm. citrate

Bile solubility

Bile , sod.desoxychollate* Blood*

Autolytic enzyme

Lyses of membrane Lysis of rbc

* *

Not inhibited

*

CAMP test lysis Cetrimide inhibition Citrate utilization Coagulase

CAMP Factor Beta lysin

Cetrimide*

Decarboxylase or hydrolyse DNA hydrolysis depolymerization

lysine DNA

Source of carbon & inorganic salt Boundcoagulase Free coagulase Decarboxylase Decarboxylase or deaminate DNAse

Gelatin hydrolysis

Gelatine*

gelatinase

Methyl Red

glucose

Acid 4.5 pH

Methyl red

Voges Proskauer

Glucose

Acetoin Neutral

 Nitrate reduction

Nitrate

nitrite

Alpha napthol 40% KOH Sulfanilic acid Alpha naphthylamine

Prepared by:

Sodium citrate Citrated plasma*

Prof. Rogelio S. Cruz CEU, College of Med. Tech. Mendiola, Manila

alkaline

Bromthymol blue

Clumping factor Clotting factor Amine Alkaline Hydrolyze DNA

* slide * tube Bromcresol purple

Liquid gelatine

*

DNA-methyl green Toluidine blue

+ clear - turbid +arrowhead - absent + growth - absent + blue - green +cellclumping + clot + purple - yellow + fading clear zone + clear zone + liquid 4 oC o - solid 4 C + red -orange-yelw

+ red - yellow + red - yellow