M319E 04.3.CF.1(1/3)
Microscope ECLIPSE 90i Instructions
Introduction Thank you for purchasing this Nikon product. This instruction manual, which describes basic microscope operations, is intended for users of the Nikon ECLIPSE 90i microscope. To ensure correct use, p lease read this manual carefully before operating the product. •
This manual may not be reproduced or transmitted in whole or in part without Nikon's express consent.
•
The contents of this manual are subject to change without notice.
•
Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may remain. If you note any points that are unclear or incorrect, please contact your nearest Nikon representative.
•
Some of the products described in this manual may not be included in the set you have purchased.
•
Make sure you have read the manuals for any other products attached to or to be used with this product (super high-pressure mercury lamp power supply, high-intensity light source, etc.).
Warning/Caution symbols used in this manual Although Nikon products are designed to provide the utmost safety, ignoring safety precautions or improper use may result in personal injury or property damage, as well as voiding the terms of the warranty. To ensure safe use, please read the instruction manual carefully and thoroughly before trying to operate the instrument. Do not discard this manual. Store in a convenient location near the product for ready reference. In this manual, safety precautions are indicated by the following symbols. For safe, correct use of the product, always follow the instructions indicated by these symbols.
Symbol
Meaning Disregarding instructions indicated by this symbol may result in death or serious
WARNING
injury.
CAUTION
Disregarding instructions indicated by this symbol may result in injury or property damage.
1
Introduction
Meaning of symbols used on the product When appearing on the product, the symbols below indicate the need for caution at all times during use. Consult the instruction manual and read the relevant instructions before attempting to use or adjust any part to which the symbol has been affixed. Caution! Biohazard This symbol found on the stage indicates the following: • WARNING: Contact between sample and the product may result in biohazard risks. • To avoid biohazard contamination, avoid touching the contaminated contaminated portion with bare hands.
• Decontaminate Decontaminate the contaminated part according to the standard procedure specified for your laboratory. Caution for heat This symbol found on the lamphouse of the ECLIPSE 90i indicates the following: • The lamp and surrounding areas (including the lamphouse) become very hot during and
immediately after a period of illumination. • Risk of burns. Do not touch the lamp or surrounding areas during or immediately after a
period of illumination. illumination.
• Make sure the lamp and surrounding areas have cooled sufficiently before attempting to replace the lamp.
2
Safety Precautions Please follow the safety precautions given below.
WARNING 1.
Intended use of the product This product is intended primarily for microscopic observations and image capture of cells and tissue set on glass sides using diascopic (transmitted) and episcopic (reflected) illumination. It is intended for use in experimentation and observation of cells and tissue in the fields of pathology and cytology in hospital and other laboratory settings.
2.
Do not disassemble. Disassembly may result in malfunctions and/or electrical shock and will void the terms of the warranty. Never attempt to disassemble any part other than the parts described in this manual. If you experience problems with the product, contact your nearest Nikon representative.
3.
Read the instruction manuals carefully. To ensure safety, carefully read this manual and the manual provided with any other equipment used with this product. Observe all warnings and cautions given at the beginning of each manual. When the digital imaging head or the D-FL Epi-fluorescence attachment is mounted on the product: The mercury lamp (or xenon lamp) for Epi-fl microscopy requires special care during handling. Make sure you have read the instruction manual for the light source (super high-pressure mercury lamp power supply or high-intensity light source).
4.
Power cord for for D-PS power supply unit and the power cord for AC adapter Use one of the power cords specified. Using the wrong power cord may result in fire or other hazards. The product is classified as subject to Class I protection against electrical shock. Make sure it is connected to an appropriate ground terminal. Refer to Chapter 7 for the power cords specified. To prevent electric shock, always turn off the main power switch (button in projected position) of the product before attaching or detaching the power cord.
5.
Heat from the light source The lamp and surrounding areas (including the lamphouse) will become very hot during and immediately after a period of illumination. •
Risk of burns. Never touch the lamp or surrounding areas during or immediately after a period of illumination.
•
Make sure the lamp and surrounding areas have cooled sufficiently before attempting to replace the lamp.
•
To avoid risk of fire, do not place fabric, paper or highly flammable volatile materials such as gasoline, petroleum benzine, paint thinner or alcohol near the lamphouse while the lamp is lit or during a period of around thirty minutes after the lamp has been turned off.
3
Safety Precautions
WARNING 6.
Mercury lamps and xenon lamps (when the digital imaging head or the D-FL Epi-fluorescence attachment is attached) The mercury lamp (or xenon lamp) used for the digital imaging head or the D-FL Epi-fluorescence attachment requires special care during handling. For safe and correct use of this system, carefully read the warnings below. Keep in mind all potential hazards. Additionally, carefully read the manual for the super high-pressure mercury lamp power supply (or high-intensity light source) and the manual (if provided) from the lamp manufacturer, then follow the instructions given therein. Hazards of mercury lamps and xenon lamps 1)
When lit, mercury (and xenon) lamps radiate ultraviolet light light that can damage your eyes and skin. Direct viewing of the light may result in blindness.
2)
The lamps contain sealed gas under very high pressure, pressure that increases when the lamp is on. If the lamp is scratched, fouled, subjected to high external pressure or physical impact, or used beyond its service life, the sealed gas may escape or the lamp may burst, resulting in gas inhalation, injury from glass, or other injury.
3)
When the lamp is lit, the lamp and surroundings will become extremely hot. Touching Touching the lamp with bare hands may result in burns; flammable materials placed near the lamp may ignite.
4)
Using the wrong lamp type may result in accidents, including bursting of the lamp.
Safety is a top design priority for Nikon products. The preceding hazards should pose no danger as long as the user observes all of the warnings and cautions given in the manuals, and uses the system only for its intended purpose. However, failure to heed the warnings and cautions given in the manuals, subjecting the system to shock or impact, or attempting to disassemble the system may result in accidents and injury. Make sure you are familiar with and adhere to all warnings and cautions. 7.
Always turn off the lamp when changing filter cubes (when the digital imaging head or the D-FL Epi-fluorescence attachment is attached to the product). When changing filter cubes, always turn off the light source of the digital imaging head or the D-FL Epi-fluorescence attachment. Leaving the lamp on may result in ultraviolet exposure.
8.
Leave the D/UV slider in the optical path (when using the digital imaging head or the D-FL Epi-fluorescence attachment). Always leave the D/UV slider (which is one of the D-ES EPI ND sliders) in the optical path when performing Epi-bright-field microscopy, Epi-dark-field microscopy, or Epi-DIC microscopy with the mercury lamphouse attached. If the slider is moved out of the path, ultraviolet light will be radiated and damage your eyes.
9.
Hazardous sample This product is intended primarily for microscopic observations and image capturing of cells and tissue set on glass sides. Check to determine whether a sample is hazardous before handling. Handle hazardous samples according to the standard procedure specified for your laboratory. If the sample is potentially infectious, wear rubber gloves and avoid touching samples. If contact occurs between a sample and the product, decontaminate the contaminated portion according to the standard procedure specified for your laboratory.
4
Safety Precautions
CAUTION 1.
Isolate the products from the power source during assembly, connection/disconnection of cords, lamp replacement, and maintenance. To prevent electric shock and/or malfunctions, always turn off the power switch(es) of the product (press it (them) so that it (they) project(s) out) and unplug the power cord from the wall outlet before assembly, connecting or disconnecting of cords, lamp replacement, and cleaning of the product and the objective.
2.
Lamp replacement precautions To avoid burns, wait at least 30 minutes after the lamp is turned off to give it sufficient time to cool. To avoid electric shock or malfunctions, never attempt to replace the lamp without first turning off the power switches for the product and the peripheral devices (press the power switch buttons so that they project out) and unplugging the power cord from the wall outlet. Make sure the lamphouse cover is securely fitted to the lamphouse after lamp replacement. Never turn on the lamp while the lamphouse cover is open. Do not break up used lamps; instead, dispose of them as special industrial waste or as specified by local regulations.
3.
Use the specified power supply supply unit, lamp, and lamphouse. Always use the specified power supply unit, lamp, and lamphouse. Using an unspecified power supply unit, lamp, or lamphouse may cause malfunction or fire. Refer to Chapter 7 for the specified items.
4.
Avoid contact with water. Never allow water to come into contact with the product, and keep the product away from liquids. Splashing water onto the product may cause a short, resulting in malfunction or abnormal heating. If water is splashed onto the product, immediately turn off the power switch (button in projected position) and remove the power cord from the receptacle. Then wipe off moisture with a dry cloth or something similar. If water enters the product, do not use; in this case, contact your nearest Nikon representative.
5.
Do not place any object on top of the product. Do not place any object on top of the product or cover it with a cloth or the like. The system temperature will rise, resulting in malfunctions.
5
Safety Precautions
CAUTION 6.
Cautions on assembling assembling and installing the product •
Take care to avoid pinching your fingers or hands during product assembly.
•
Scratches or fouling such as fingerprints on optical components (such as lens and filters) will degrade microscope images. Be careful to avoid scratches or direct contact with the lens and filters.
•
The main unit weighs about 12 kg. Grasp the main unit by the recesses at the front of the arm and at the bottom of the main unit.
7.
•
Remove all attachments (if mounted) from the microscope before carrying the microscope.
•
Do not install the product in a locker or cabinet.
Remove any covers from the product before switching on. Do not use the product while covered with a cloth, etc., as this will give rise to abnormal heat, which could cause a fire.
8.
Caution concerning long, sustained observations To relieve fatigue resulting from long observation sessions, limit continuous observations to one hour. Take at least 10- to 15-minute breaks between observation sessions. Adjust the layout of other equipment and the height of your chair.
9.
Disposal of the product To avoid biohazard risks, dispose of the product as contaminated equipment according to the standard procedure specified for your laboratory.
6
Notes on handling the product
Notes on handling the product 1.
Handle the product gently. This product is a precision optical instrument and requires gentle handling. Avoid subjecting it to sudden impact and shocks. Even relatively minor impacts are capable of affecting the precision of the objective.
2.
Weak electromagnetic waves The product emits weak electromagnetic waves. Do not place precision electronic devices near the product to avoid degrading their performance. If TV or radio reception is affected, move the TV or radio farther away from the product.
3.
Scratches, dirt, and foreign particles on the lens Scratches or fouling such as fingerprints on optical components (such as lens and filters) will degrade microscope images. If these parts become dirty, clean them as described in chapter "6. Care and Maintenance" at the end of this manual.
4.
Dirt on the lamps Never touch the lamp with bare hands. Dirt or fingerprints on the lamp will result in uneven illumination and reduce the service life of the lamp. Always wear gloves when handling lamps.
5.
Installation location This product is a precision instrument. Use or storage in inappropriate environments may result in malfunctions or poor performance. Consider the following factors when selecting an installation location: •
Select a vibration-free location. Install the product on a level surface.
•
Install the microscope and power supply unit at least 10 cm away from walls. Additionally, allow at least 10 cm between the microscope and the power supply unit.
•
Choose a location less exposed to hazards in the event of collisions, earthquakes, or other potential disasters. To keep the product from falling, use strong rope or other means if necessary to secure it to the working desk or to another heavy, stable item. To fix the product, there are two M6 screw holes (one at the right and the other at the left) behind the recess at the base of the main body (back side).
•
Avoid locations exposed to direct sunlight, locations immediately under room lights, and other bright locations.
•
Avoid locations with excessive dust.
•
To avoid splashes, do not use the product near water.
•
Make sure the ambient temperature is 5 to 35 °C and humidity is 85% or less. Installing the product in hot, humid locations may result in mildew formation or condensation, impairing performance or generating malfunctions.
•
Do not install the product in a locker or cabinet.
•
Select a layout that allows easy removal of the power cord from AC inlet of the power supply unit in the event of an emergency.
•
Room lights just above the product may enter the objective as extraneous light. If possible, switch off room lights directly above the product when making observations.
•
Do not set up the microscope on a desk mat or similar pad.
7
Notes on handling the product
6.
Focusing knobs •
7.
Never turn the focus knobs on the left and right sides of the product in opposite directions at the same time. Doing so may damage the product.
Protect the ports from dust and extraneous light (when the trinocular eyepiece tube, the digital imaging head, or the C-TE ergonomic binocular tube is attached). To keep out extraneous light and dust, always attach the supplied cap to any port not currently in use.
8.
Handling of filters (when the digital imaging head or the D-FL Epi-fluorescence attachment is attached to the product) •
Interference filters (especially excitation filters, which are exposed to strong light) degrade over time. Replace them after the appropriate hours.
•
Filter characteristics may alter if the filter is exposed to high humidity. To prevent changes in or degradation of filter characteristics, avoid using or storing the filters under conditions of high humidity or high temperature. Avoid subjecting filters to rapid temperature changes. When a filter is not in use, store in a desiccator or hermetically sealed container with a drying agent.
•
The filters in the nine types of filter cubes listed below offer sharp, high-resolution waveform characteristics superior to normal filters. However, due to their sophisticated coatings, they must be handled with special care. In particular, take care to avoid abrasion from cleaning. (Follow the procedure described in section "1. Lens Cleaning" of chapter "6. Care and Maintenance.") Single-band filter cubes: DAPI, FITC, TxRed, GFP Multi-band filter cubes: F-R, F-T, D-F, D-F-R, D-F-T
9.
Handling of the D-FLD dark-field illumination cube (when using the digital imaging head head or the D-FL Epi-fluorescence attachment) There are two wings in front of the D-FL Epi-fluorescence attachment, to prevent light leakage. Do not apply any pressure onto the wings, as malfunction may result.
10. Unpacking and unclamping 1)
Check the contents to ensure that the package contains the following: ECLIPSE 90i Tools
2)
x1 Hex driver
x2
Allen key
x1
Accessories
Sticker (blind cap)
x1
Cable
Lamp cable
x1
Control cable
x1
USB cable
x1
Before turning on the power, remove the retaining screw located on the front side of the stage elevating section.
3)
Do not discard the packing case, as it may be needed for future transportati on.
8
Abbreviations used in the manual
Abbreviations used in the manual The product names and abbreviations used in this manual are given below. The manual uses the following abbreviations:
Name of device
Abbreviation
Microscope ECLIPSE 90i
90i
C-ER Eye Level Riser
Eye Level Riser
C-TE Ergonomic Binocular Tube
Ergonomic Binocular Tube
C-TEP DSC Port for Ergonomic Binocular Tube
DSC Port
D-FL Epi-Fluorescence Epi-Fluorescence Attachment
Epi-Fl Attachment
D-DH Digital Imaging Head M
DIH-M
D-DH-E Motorized Digital Imaging Head E
DIH-E
D-PS 90i Power Supply
Power Supply Unit
D-LH Precentered Precentered Lamphouse
Lamphouse
DS Camera Head DS-5M/ DS Cooled Camera Head
Camera Head
DS-5Mc DS Camera Control Unit DS-L1
DS-L1
DS Camera Control Unit DS-U1
DS-U1
DS Camera Cable
Camera Cable
Super High-pressure High-pres sure Mercury Lamp Power Supply
Mercury Lamp Power Supply
Super High-pressure Mercury Lamphouse
Mercury Lamphouse
D-CH 90i Condenser Holder
Condenser Holder
D-CUD-E Motorized Motorize d Universal Condenser Dry
Motorized Motoriz ed Universal Condenser
D-N7-E Motorized Septuple Nosepiece
Motorized Nosepiece
D-NF-E 90i Motorized ND Filter Unit
Motorized ND Filter
9
How to use this instruction manual
How to use this instruction manual This instruction manual is composed of two parts, as below: Manual 1 "Microscopy" describes basic microscope operations that you must follow. Please read this manual carefully before operating the product. Manual 2 "Reference" describes the operations of each attachment. Please read an appropriate section as necessary.
Manual 1
Title
Importance
Content
Microscopy
Must be read
Safety precautions Microscopy, Assembly, Troubleshooting, Troubleshooting, Care and maintenance, Technical Specifications
Manual 2
Reference
As necessary
Detailed operations operatio ns of attachments
10
Using microscopy manual
Using microscopy manual The part names and microscopy procedures are described in two separate sections, corresponding to specific configurations configurations of 90i and accessory devices. Confirm the combination of the 90i and accessory devices you are using, then refer to the appropriate section.
Combination
Description
Bright-field set
See Chapter 1.
(Eyepiece tube mounted
(For operating
directly on 90i)
procedures and detailed information on
(Camera and monitor are
individual parts, refer
optional.)
to the separate Reference manual.)
90i + DIH-M
See Chapter 2.
(Camera and monitor are
(For operating
optional.)
procedures and detailed information on individual parts, refer to the Reference manual.)
11
Contents
Contents Introductio Introduction n ......... .................. ................... .................... .................... ................... ................... .................... .................... .................... ................ ...... 1 Warning/Caution Warning/Caution symbols used in this manual .................................... ..................................................................1 ..............................1 Meaning of symbols symbols used on the product ............................... .................................................................... .......................................... ..... 2
Safety Precautions Precautions ......... ................... .................... ................... ................... .................... .................... .................... ................... ................ ....... 3 WARNING.......... WARNING.................... .................... .................... .................... ................... ................... .................... .................... ................... ........... .. 3 CAUTION........... CAUTION..................... .................... .................... ................... ................... .................... .................... .................... .................... ............ 5 Notes on handling handling the product product ......... ................... ................... ................... .................... .................... .................... ................... ........... 7 Abbreviati Abbreviations ons used in the manual...................... manual................................ ................... ................... .................... ................... ............. .... 9 How to use this instruction manual .............................. .............. ................................ ............................... ......................... .......... 10 Using microscopy manual....................................................................................11 Chapter 1 Part Names and Microscopy Microscopy Procedures Procedures (Bright-Field (Bright-Field Set) ......................... ................. ........ 14 1.1
Names of Main Components ....................................... ............................................................................... ........................................ 14
1.2
Names of Parts Used to Make Adjustments............................................ Adjustments........................................................... ............... 15 1.2.1 Right view..................................................... view.......................................................................................... .............................................. ......... 15 1.2.2 Left view.......................................................... view............................................................................................... ........................................... ...... 16 1.2.3 Rear view (without accessory device) .................................. ............................................................. ........................... 16 1.2.4 Ergonomic binocular tube tube with with camera camera attached............................... attached............................................... ................ 17
1.3
Bright-Field Microscopy..................................................... Microscopy...................................................................................... ................................. 18
1.4
Photomicroscopy............................................................................................... Photomicroscopy....................................................................................... ........ 22
Chapter 2 Part Names and Microscopy Procedures (With Digital Imaging Head Mounted) .............................. ............... ............................... ..................... .....24 24 2.1
Names of Main Components ....................................... ............................................................................... ........................................ 24
2.2
Names of Parts Used to Make Adjustments............................................ Adjustments........................................................... ............... 25 2.2.1 Right view..................................................... view.......................................................................................... .............................................. ......... 25 2.2.2 Left view.......................................................... view............................................................................................... ........................................... ...... 26 2.2.3 Rear view (without accessory device) .................................. ............................................................. ........................... 27
2.3
Bright-Field Microscopy..................................................... Microscopy...................................................................................... ................................. 28
2.4
Epi-fluorescence Epi-fluorescence Microscopy .................................. ...................................................................... ............................................. ......... 41
2.5
Photomicroscopy............................................................................................... Photomicroscopy....................................................................................... ........ 52
Chapter Chapter 3 Assembly Assembly ......... ................... .................... .................... ................... ................... .................... .................... .................... .............57 ...57 Chapter 4 Replacing Consumables .............................. ............... ............................... ............................... .......................... ........... 74 4.1
Replacing the Lamp .................................. ......................................................................... ......................................................... .................. 74
Chapter 5 Troubleshooting...................................................................................75 5.1
Optical............................................................................................................. Optical.................................................................................................... ......... 75
5.2
Electrical.......................................................................................................... Electrical.................................................................................................... ...... 77
Chapter 6 Care and Maintenance..........................................................................84 6.1
Lens Cleaning.................................................................. Cleaning................................................................................................... ................................. 84
6.2
Cleaning the Product ................................. ...................................................................... ........................................................ ................... 84
6.3
Disinfecting the Product ................................... ........................................................................ .................................................. ............. 84
6.4
Storage................................................... Storage....................................................................................... ......................................................... ..................... 85
6.5
Periodic Inspections (fee charged)................................ charged)................................................................... ....................................... .... 85
Chapter 7 Technical Specifications .............................. ............... ............................... ................................ .......................... .......... 86 7.1
Specifications ................................... ....................................................................... ................................................................ ............................ 86
12
Contents
13
1
Part Names and Microscopy Procedures (Bright-Field Set)
1.1
Names of Main Components
Eyepiece
Eyepiece tube
Nosepiece Objective Specimen holder Power supply
Stage
Condenser
Lamphouse Microscope (main body)
14
Chapter 1 Part Names Names and Microscopy Microscopy Procedures Procedures (Bright-Field (Bright-Field Set) Set) 1.2
Names of Parts Used to Make Adjustments
1 1.2
Names of Parts Used to Make Adjustments
1.2.1
Right view Optical path switching lever
Diopter adjustment ring
Y stage knob Focus knob
X stage knob (Provided on the left side in certain products)
Aperture diaphragm open/close switch Field diaphragm open/close switch
Filter IN/OUT switches
Coarse focus switch Not used
15
Chapter 1 Part Names Names and Microscopy Microscopy Procedures Procedures (Bright-Field (Bright-Field Set) Set) 1.2
1.2.2
Names of Parts Used to Make Adjustments
Left view
Condenser centering screws
Condenser focus knob
Focus knob Not used Objective selector switch Power indicator Coarse focus switch Escape switch
1.2.3
Brightness control knob
Rear view (without accessory device) Tool storage compartment
Cable guide
Motorized ND filter / lamphouse mounting section
16
Preset switch
Chapter 1 Part Names Names and Microscopy Microscopy Procedures Procedures (Bright-Field (Bright-Field Set) Set) 1.2
Names of Parts Used to Make Adjustments
1 1.2.4
Ergonomic binocular tube with camera attached
Camera cable connector Camera head
C mount Camera fine focus adjustment screw Camera centering screws
Mounting direction fixing screw
17
Chapter 1 Part Names Names and Microscopy Microscopy Procedures Procedures (Bright-Field (Bright-Field Set) Set) 1.3 Bright-Field Microscopy
1.3
1
Bright-Field Bright-Field Microscopy Turn on the power switch of the power supply unit. Additionally, switch on power for peripheral devices (camera control unit, PC, etc.). If you are using a PC, launch "i Series Support Tools" and other required software.
When the power switch is turned on, the LED located above the switch will light and a long buzz will sound. Initialization will then begin. When initialization initialization is complete, the buzzer will sound once again. The Power ON indicator flashes during initialization, initialization, then turns green when initialization initialization ends.
Press the power switch to its ON position.
Initialization Initialization normally requires 10 to 20 seconds.
2
Press the preset switch.
Press the preset switch.
3
Move the ND8, ND32, and NCB11 filters into the optical path.
NCB11 ND32 ND8
18
Chapter 1 Part Names Names and Microscopy Microscopy Procedures Procedures (Bright-Field (Bright-Field Set) Set) 1.3 Bright-Field Microscopy
4
5
1 Push in the optical path switching lever to direct the full optical path toward the binocular section.
The full optical path directed toward the binocular section
Push in the optical path-switching path-switching lever.
Raise the condenser to the uppermost position.
Raise the condenser using the condenser focus knob.
6
Set the 10x objective into the optical path.
Press the objective selector switch to set the 10x objective in the optical path.
7
Fully open the field diaphragm and aperture diaphragm. (When using the 1-100x condenser, move the top lens of the condenser into the optical path.)
Fully open the aperture diaphragm using the aperture diaphragm open/close switch.
Fully open the field diaphragm using the field diaphragm open/close switch.
When using a condenser other than the motorized universal condenser: Fully open the aperture diaphragm using the aperture diaphragm lever.
19
Chapter 1 Part Names Names and Microscopy Microscopy Procedures Procedures (Bright-Field (Bright-Field Set) Set) 1.3 Bright-Field Microscopy
8
Set a specimen and move the portion to be viewed into the optical path.
Set a specimen and secure in place using the specimen holder.
Move the portion to be viewed into the optical path using the XY stage knobs.
9
Focus on the specimen.
Adjust the focus using the focus knobs.
the diopter and the 10 Adjust interpupillary distance. Refer to "11. Diopter Adjustment" and "12. Interpupillary Distance Adjustment" in the separate Reference manual.
11 Focus and center the condenser. Refer to "14. Adjustment of Condenser Position" in the separate Reference manual.
Focus the condenser using the condenser focus knob.
Center the condenser using the condenser centering screws.
Field diaphragm
Field diaphragm
Eyepiece field of view
Eyepiece field of view
20
Chapter 1 Part Names Names and Microscopy Microscopy Procedures Procedures (Bright-Field (Bright-Field Set) Set) 1.3 Bright-Field Microscopy
1
to the desired objective 12 Switch and view the specimen. • Adjust the brightness with the ND filter IN/OUT
switches. (When the motorized ND filter is used, the brightness is automatically adjusted when objectives are switched.)
Select the desired objective using the objective selector switch.
• Switching of objectives may result in an
out-of-focus image. When this happens, correct the focal deviation. For details, refer to "5.11.1 Setting Up the
When using a condenser other than the motorized universal condenser:
Up/down Focus Motion" in the manual for the "i Series Support Tools" software. • If a condenser other than the motorized universal
condenser is used, adjust the aperture diaphragm each time you switch the objective. Aperture diaphragm: 70% to 80% of the numerical aperture (N.A.) of the objective
Adjust the aperture diaphragm using the aperture diaphragm open/close switch.
off the power switch of the 13 Turn power supply unit after completing observations.
Press the power switch again so it is in the OFF position.
21
Chapter 1 Part Names Names and Microscopy Microscopy Procedures Procedures (Bright-Field (Bright-Field Set) Set) 1.4 Photomicroscopy
1.4
Photomicroscopy For detailed explanations of the camera, photomicroscopic software, software, and PC, refer to the operating manuals provided with the respective products. The following instructions assume a DS camera head DS-5M and DS-L1 camera control unit.
1
Adjust the microscope for proper image observation. See the directions given earlier in "1.3 Bright-Field Microscopy."
2
Adjust the camera head attaching position until the image is displayed properly. (1)
Adjustment based on stage motion direction Loosen the mounting direction fixing screw on
(B)
the C mount, and adjust the camera position so that moving the stage left-right moves the
(A)
image on the monitor in the opposite direction. After making the appropriate adjustments, tighten the screws firmly. (2)
Adjusting (A) and (B)
Focus adjustment If the image viewed through the eyepiece appears to be in focus but the image on the monitor is out of focus, turn the camera fine focus adjustment ring on the C mount until the image on the monitor is in focus. Note that such out of focus situations can also indicate incorrect diopter adjustment. Be sure to make proper diopter adjustments. (Refer to the separate Reference manual.)
(3)
Center the camera Turn the right and left centering screws to align the image seen through the eyepiece with the image on the monitor.
3
Camera centering screws
Camera fine focus adjustment ring
Mounting direction fixing screw
Make camera settings. For a detailed discussion of this topic, refer to the operating manual provided with the camera. When using the DS-L1, you must choose and enter at least the following information: • Folder for data storage. • Name of file to be saved. (You can select "Auto.") • File format and file size. • Date and destination of data
22
Chapter 1 Part Names Names and Microscopy Microscopy Procedures Procedures (Bright-Field (Bright-Field Set) Set) 1.4 Photomicroscopy
4 5
1 Select the camera scene mode suitable for the microscopy method. Set the camera white balance. To adjust white balance, press the WB button while capturing an image of a clear section of a specimen slide. (For fluorescent fluorescent photomicrography, adjust white balance under normal lighting conditions before shooting.)
6
Capture and save images. Position the specimen. Refocus. Adjust image brightness using the camera exposure compensation function. Check the image using the Freeze button. If the image is acceptable, press the CAPT. button to save the image. (The operating procedure differs if DF/FL scene mode is selected. For a detailed discussion of this topic, refer to the operating manual provided with the camera.)
23
2
Part Names and Microscopy Procedures (With Digital Imaging Head Mounted)
2.1
Names of Main Components
Eyepiece
Digital imaging head (The photo shows the DIH-E.) Lamphouse for Epi-illumination (The DIH uses a mercury lamp, xenon lamp, or high-intensity halogen lamp as a light source. It needs a separate power supply unit. Before using, be sure to read the instruction manual provided with the lamp source equipment.)
Motorized nosepiece Objective
Power supply unit
Specimen holder Stage Motorized universal condenser
Lamphouse Microscope (main body)
24
Chapter 2 Part Names and Microscopy Microscop y Procedures Procedur es (With Digital Imaging Head Mounted) 2.2
Names of Parts Used to Make Adjustments
2.2
Names of Parts Used to Make Adjustments
2.2.1
Right view
2
With the DIH-E mounted C mount adapter C mount adapter retaining screw
Excitation ID tag pocket
Filter cube mounting section (inside)
Y stage knob X stage knob
Focus knob
(Provided on the left side in some products)
Aperture diaphragm open/close switch Field diaphragm open/close switch Filter IN/OUT switches Coarse focus switch Excitation method switch
Shutter switch With the DIH-M mounted Optical path switching lever (1) (only on DIH-M) Optical path switching lever (2) (only on DIH-M)
Zoom knob (only on DIH-M)
Excitation method switchover turret (only on DIH-M) Aperture diaphragm open/close lever (for Epi-illumination) Epi-illumination) (only on DIH-M)
Field diaphragm open/close lever (for Epi-illumination) (only on DIH-M)
Field diaphragm centering holes
25
Aperture diaphragm centering holes
Chapter 2 Part Names and Microscopy Microscop y Procedures Procedur es (With Digital Imaging Head Mounted) 2.2
2.2.2
Names of Parts Used to Make Adjustments
Left view
DIH power indicator
ND4 filter slider ND8 filter slider ND16 filter slider
Condenser centering screws
Condenser focus knob
Focus knob DIH-E optical path selector switch Objective selector switch DIH optical path position indicator Power indicator
Coarse focus switch Escape switch
Brightness control knob
26
Preset switch
Chapter 2 Part Names and Microscopy Microscop y Procedures Procedur es (With Digital Imaging Head Mounted) 2.2
2.2.3
Names of Parts Used to Make Adjustments
Rear view (without accessory device)
2
Tool storage compartment
Cable guide
Motorized ND filter / lamphouse mounting section
27
Chapter 2 Part Names and Microscopy Procedures (With (With Digital Digital Imaging Head Head Mounted) 2.3 Bright-Field Microscopy
y p o c s o r c i M d l e i F t h g i r B
e e d h h t n i t t , n a e d , e u n e h s t t e i a l h e r s t o e h g u e r r t n s T p h i t . n c i s s a s o o u u w e o w c c t n . d e e i a e e y e d d v r r a o e u e m t p d i s m m d s m . ' v l m l i a o e e r n 1 c a 0 b e m . r , L s p e o y o d S 9 S s l b t e D e a f l s l e t r D l h u e u c d b t d a e v C a e a n d n t e h a i a f x l s . h a t o i e r e b i , m a t s r a i n D . h o d n d a s p r e E s g d e i e o o p w n e m t i t t w i n o L s e e t e f i n c g l o i o h o t o i o t s v u t t e n l n t s s a r y e a o f c t o t " e h f i s d l s a o e c n s c e t l u e , c f n . i i p s n o h t n r n d o e e u . o t e a f k e n c i h e e i T a e s u c r , t s E o b c s t h n b k l r t e i o s o c r o n u r H t o n v t e o t c I r u a t a p o a c l i r n c t n p i e u t e i e e s m D f h r u t p r ' t s e e e e S e s i m n a t h h r d n h s m t " e w i o i e t u n t e , h t f n i s h t s f u o r r s m e t e o l , i f o e a d h i s 0 s o " S e r t , h y o u f p 9 W 0 " i h a i 9 s t a T " t d u . e a b t e l n t e l t e r n o h h s b o a n a e e d t o T S o n o t v . e u p T f e S o d l Z m n h p i t o E t e e a i o t c u r i r p s h n " m o t n e S o a t u o a p o l f o t s i n n t p r i e b i e a o t s e f i n c u h e u r p e e o t s m S e i e d d C s s o t h e e o g n r a t S i e i u a h e f i o s f " m n t d m " m a r o p e s n d i r p c s e e o d b S u o g i r u t f 6 n t a e s i n d r y n s a e p i i h e d s " t h t " n b t t b n S e c r e Z c i a s e r i n h a e d 1 o E s r t o i L i p t o c e t " o e s S y t d l f a t a a S d o a l e e l h v g c D e a t s h r d . e e " n o f t u d t d n e i a r I s n d o h s s c e c e t h t e a i i e h b u i " o d v . t r y o h l t o i m p t e e i e c t e a f d 0 s l e d s c u r a a i i 9 e t n n e " m p e o p s b n l t i e e m d s h t S o e h u o c o n d p a t c h e c i r e e l s i t i Z o r s t s t . r e n b t w n n a s o i i t n b E a e e a r " o s s n s o e e l i t r t c e r v r i c i s c f n g i e e C i f p n o d c u n h " y i s m g o n s o t d o n a e e a e i t b p o e c " h e r o g t d d m t l c h n t t h o t e t m i o s p n t e n t e s a o n r o t r t a o m p c s i e g c c o o e t i n t v u e t m d n o i a t i h c r c n ) e o d h t i 1 t d o n C c n t e a s t i r a d h i o L n o e t o e s d d - t " n a p r e c v c k i s n c a e g e S e o e s a i h n l e a h t n D s h D e I e u r r M T S m R r W f ( U t o f e • • • B •
3 . 2
28
Chapter 2 Part Names and Microscopy Procedures Procedur es (With Digital Imaging Head Mounted) 2.3 Bright-Field Microscopy
r 1 w e L - o p S h e t . D f 1 o L g e S n d i i s D e s " h u " t e n n t h o e s h c s t h e i r s W P w
2
l o r t s . n t r e e a i r o t r a s e C w S t i d i f n " o a g , e " s h C n P t l s i f s h e o o o u t " l T t o r r n n o t o n p e n o p r C u h T u i " S W
e p o c s o r c i m i 0 9
を e h チ t N ッ f O o イ s t h i ス c t i o 源 t w 電 t s i n r の u e イ w y l ラ o p p プ p e u . サ む s h n t o r ー 込 i s e t i s ワ し w s e o o パ 押 r P p p
. s d e c n i a v i e 0 d 9 l e a r e h t h t p r i a r t e S p
l r o r t o f n r o e c w a r o e p m n a o c h ( c s t i e c . w i s v ) . e , c d t y l l l e a a r , n e C o h P i t i i p , d r t i d e n A p u
r i e " h h t c o n d u n a a l , " C l s P o . a o e r g T a n t r w i s o t u p f p o e u s r S a d e s i r u e o i u r y e q f S e I r
o t 0 d 1 z n e e d z h s n n e u n r h t e u e r b u l t i o e l i s s t r u e g i N s l g q i v n w n l . e o n i o l o i O s s r g n h b r n t w e d o c i r y i t r l n i a a t a r u l z e w u i d a w d z o t e a z l e n i s t d z a n P n l i u m a a t r o r s e i i i b e e e a c t h t o t n i h t i e h a n o h w l n n h T s , i g I z o i . n s l . n a t p D l e l . o d l a n o f i l , i i E i h d i e e a r t t i t a n h L w n t W g t o a n i o t z c i e e h u z . l a i l o l h c s n p a n t e n a i a e i s e c i e , t i l l g m c i t i 0 h n i h t w i e o n d n 2 n i n w I W o s w b c o i
1 29
Chapter 2 Part Names and Microscopy Procedures (With (With Digital Digital Imaging Head Head Mounted) 2.3 Bright-Field Microscopy
1 L S D g n i s u n e h W
e h y a t l p n s o o i f n k d I c o i l t C . c n I t u e h e M n r e g i c h e R s t m ) 2 (
. y ] s F a B E [ M k c A i l C c e , u h t n n e O m ) 1 (
l o r t n o C i g n i s u n e h W
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. ] d e l i F t h g i r B [ k c i l C
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k c i l C e e h k t a e m v o d b n a a d , e h t c t i a c o w s l t D e E s L . e e t s r h p t h e t g l h a i t h s t h c s e i r t e r u w P s s
d e g e r n m a i r s w o g l o f r i n l o e t f p t e e e s h b e t t h y o n T b . y n t a i l c n g n u i n i o d i r t 0 9 o a r e c c e h a p t . o e s d p s m i a t e h o r m T f s
l f a o s r n e o e i v b e t a i n h n n t u a r i c b d d o e e d l m z i z o o o i r r c r h o o t t a t t e n o g o o m C n m m y i i , d s E p g u - n o i n c s n H a . s u e I , r o h D e r c e s , e c d i e w i i n 1 0 p e g p m i L 9 e d T e n a - e s n o o h h S h T c D t n c
y p t . o h y c g p s i o o r c r b c s i o o r t m i c e d o m h t h d l t t e e e i S m f
2
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s r o s l e o n o c t h t t s . ) g y e t i r e b i l b g i a e b t e l h i t c h t o u v e t v d s p e o i r u m j a h d l c p A ( a e r
3 30
Chapter 2 Part Names and Microscopy Procedures Procedur es (With Digital Imaging Head Mounted) 2.3 Bright-Field Microscopy
1 . L - b e 1 L - s S t S i h o D n D t e r n h o g a c t f t n i i n m n o o s t i u r f i ) u a 0 . r d e e 9 n o n o p m e i r h t e i o t a s f r h h e r e e s p T W ( p U o
2
l e ) o . s n r U o t e . t i n b l a r o o t r e t o p C n o i n n o s C i a i c t h g n m r n o o o i t r f f s i t a i r d n u e e u p m o r i n s 0 o 9 e i f h r e h ( T e h p t W
e p o c s o r c i m i 0 9
n o s e h c t i w s . e T d U i O s / N N I I e r h e t t l o i f t e i 0 h t 9 t e e h S t
1 1 2 B 3 8 C D D N N N
, o 2 t n 3 i D s . N r e h , t t 8 l a i D f p N 1 l a e 1 c i B h t t C p e N o v d e o n M a h t
4 31
Chapter 2 Part Names and Microscopy Procedures (With (With Digital Digital Imaging Head Head Mounted) 2.3 Bright-Field Microscopy
1 L S D g n i s u n e h W
.
k c i l C
l o r t n o C i g n i s u n e h W
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. e 1 L b - s t S i o D h n e t r n h o a t f c t i n m n o o i r u t f i a . r d 0 ) e e 9 n o p m e i o r h t o t a s f r i r e e h e s p T p U o (
) . e n s o U t i e . a b l r o t t r o n e p n o o n s C i a i c t h n m r o o o i r f t f t a r d i e e n u p m o r i 0 o 9 s f i r e h e T p t h (
. ] r a l u c o n i B [ ⇒ k c i l C
E H I D d n a i 0 9 e h t g n i s u n e h W
l r a l c a i t u p c o o n l l i u b f e e h t . h t o n t t o c h i t e t r i a c e D p s
h O N t a I B p h c e l t . a i h c w t s i t s t t h p r a g o e i h l t e v r o o o s h e t t ) a g s n c H s a I i e d r h D n P c ( i O N I B
M H I D d n a i 0 9 e h t g n i s u n e h W
d n a r e h . p t s p a r u p e v h l a t e c l o t i b p g n t o u r i h o e c t l i l w u o w P l s e h t n h d o t i r t a a c p w e l o s a t r c a i d t e l p t u o c c o l e l r n u i i F d b
5 32
Chapter 2 Part Names and Microscopy Procedures Procedur es (With Digital Imaging Head Mounted) 2.3 Bright-Field Microscopy
1 e ] 6 C n I o [ M k h c g e i l u h c o t r , h n . ] t o ] A I ] A 1 D I [ [ D f o o t [ t o t ) a t h t e n t o i e s t e i r s t u i s o s . s n o s p i e u A k n t i I r a e f D f o M m I (
1 L S D g n i s u n e h W l o r t n o C i g n i s u n e h W
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k c i l c , ] A I D [ o t t . a t ] h A e t s I D e t r o [ u s . n ⇒ s i e ] A k I t i a D f M [ I
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h e t s r c h e t t i t h l i g f f i w s o l a r e . d o t ) o t b u u h h a c o t t i c r h a e d t e i p m t n l i l i w a n e f n c o i a i o h t i t t t t p i o a u t i t a o s c h e h o p x t t h e o i e t e s w h o t t h ) n t n t i o E i r s B t e i e s U s s b e n u r C o i P ( p ( c
. e 1 L b - s t S i o D h n e t r n h o a t f c t i n m n o o i u r t f i a 0 . r d ) e e 9 n o p m e i o r h t a t o s r i f r e e h e s p T ( p U o
2
) . e n s o U t i e . a b l r o t t r o n e p n o o n s C i a i c t h n m r o o o i r f t f t a i r d e e n u p m o r i 0 o 9 s f i r e h e T p t h (
M H I D d n a i 0 9 e h t g n i s u n e h W
e . b h u t c a r p e l t a l i c f i t e p h t o e e h v t o m m e o r R f
6 33
o e t n h i d t e o t r b h e t u s e n c i r m e d t n l i n o a f i t t a . a t h t i e t c r r u o a x t u h p e t i l r e e w a h v c t i t o n e o p i o t h c t i a t t i s e o w o h R s p t
Chapter 2 Part Names and Microscopy Procedures (With (With Digital Digital Imaging Head Head Mounted) 2.3 Bright-Field Microscopy
1 L S D g n i s u n e h W
2 C ] 1 I M [ e k c l h i t c , n ] o . A ] I A D I [ D o t [ t o t a t h t e t e s e r s t o u i s s n . s ) i e u A k n t i a e f I D M m I (
l o r t n o C i g n i s u n e h W
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) . e n s o U t i e . a b l r o t t r o n e p n o o n s C i a i c t h n m r o o o i r f t f t a i r d e e n u p m o r i 0 o 9 s f i r e h e T p t h (
d r a w o . t s b n o r n u k t s r u e g c n o o f l r e o s n n t e i l d i t n o n c u e k c h a t b n r e u h T t
d y e l l n r a c i u t t s a i i m . o 0 t 9 u n o e a i i h s t t s f i r o o e p r s y e n t w e p o d m p n e o e c n h t e a h o n t e , t t h n e W o s
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7
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8 34
Chapter 2 Part Names and Microscopy Procedures Procedur es (With Digital Imaging Head Mounted) 2.3 Bright-Field Microscopy
e l r o o h f ( e ] 6 n x [ o 0 h k 1 g c u i l h o c t r , i h u w t n d ] e l e 1 l [ m a t 1 s f o C n I i ) e M n v i e i o t c . h t i e t s j n o b O p o
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r o t c e l e s e v i t c e . j ) b J o B e O ( h t s h c s t i e r w P s
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, r r r e e e e s h s n t l h t n e o a s . e r r e g r d d e e n e n n o h i v s v t s e o n i n n u c c l e u e d e x h n d p m m 0 t . o e o g g 0 f h c z y a 1 o t r a r r l - s a a i l h h o 1 n u p p p g t f e e l n o , i a i a h l a i s m r d d t c u p i e e s e g o t r e r p n h n u n t o i u t e e e t t s h d r r u t e h n e e h W a n n e t o p p h c a a e v o * t h o t n W m i
. d m l e g i r f d a h e n p h a t a i d n m e g e p a r o r t u h y r l l p a e p u i F d a
0 1 35
Chapter 2 Part Names and Microscopy Procedures (With (With Digital Digital Imaging Head Head Mounted) 2.3 Bright-Field Microscopy
1 . L - b e 1 L - s S t S i h o D n D t e r o h f g n a t c n n m t i i n o o s t i u r i f u a 0 . r d ) e e 9 n n o o p m e i r t h t e i a o s f r h h r e e e s p T W ( p U o l ) o . e n r s o t e U t i . a n b l r o o t r t e o p n o C i n n o s C i a i c t h g n m r n o o i t o r f f s i t a i r d n u e e u p m o r i n s 0 o 9 e i f h r e h ( T e h p t W
e p o c s o r c i m i 0 9
t i . e r r e u d c l e o s h d n n e a m i n c e e p m i c s e e p h s t a g n t i e s S u ) 1 (
d e e h t w e g i n v i s e u b h o t t a n p . o s i l a t r i c b o o t n p p k e o h e e g t h a e t t v o s o t Y n M i X ) 2 (
e . h d e h t h e t n t w a o e e p i l n v a e o v c e i m t m b i c d o p e n t o p a n e s , o h t i a e t g o t a r t e t o n S s p i
1 1 36
Chapter 2 Part Names and Microscopy Procedures Procedur es (With Digital Imaging Head Mounted) 2.3 Bright-Field Microscopy
1 L S D g n i s u n e h W
k c i l c r , o u n e m / 1 C I M e h ⇒ t n O
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, e a t r x o h x t h e 4 n t 4 t s i s r i o n o n r t e w n t e o f n e e i a i a i n h , n o r h h t i A m e h t t o i g t t t p . t e k c t s i s h n v i a c n g t s s t u t l o b a l n s u i e a t e t s a l i c c r f i e h f p h f j e d k f a t t s i c e h i i l o o v u l i a t w n b o n c ( c t a . h t w n i c c n n o e e o o t i x f h i i a e i r o i v t p h v t 0 t a o d o s t e f n t a o t t a 1 a g c i c i c e u c u s ) p a c g j a l e f e f a u n e j i h i t o m n a n t f i b n r c n e g o i o c b t i t o k o g e t o g o t h n t t i e t c a a t s i e u p n p u l n fI b n u n o b s a m i a c a m i b
. l a d a e u n e r t h a , n m o e s ” n s e g u r e n c i o f k f e o c r . i l t e c u t A a e r r . a o 2 k f 0 p c i l e 1 e c B “ s
” d s e e u t c r h n o t e o e f s u h g t o n o 1 n F t L s u e m - i r e A A c k e c t s M f i i l a e e . r v h 2 5 c i e / t 0 e , t p c 1 t o M 1 g “ c 5 U a n d a s - - n i r c o . k a a o r S c p t M D i e s c l r e . i 5 t u u e / c c , s l m b h a e u o e r n f e t M o t h 5 o t o n h t h - F t f t t S A u e u n a f i I w D a B b o m
e h t g n i s u n e m i c e p s . e s h b t o n n o k s s u u c c o o F f
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2 1 37
, e n a t r x o t x t h o F n e 4 4 t t a s A o s i o e u r n n n o b g a t n e e i a i n , h i o r h t h n t i h i m e t p . F i t e v g k c t t a s h n A a s i v i c . n g t s t t a s t l h o s n e a t e c c i u i r s l i n l f i e h f p e f j e d a k f e v a h t s i c h i l i v t t o b n g u l e t w o a t i c ( c t c n o a a h n c i c g e n e i o o o x h n t f j a i n r o i v t p i b t o t t o d o n t s k o a 0 t e f a o t 1 a c u s ) e c i c e u l c p i i a l u e f a u n j f i h c o c n m a i a b t f b n n t e g o i r r c t i o o g g h i n t e h e t e c F o t t t i a s t f e u p n a t p A u fI b n a A w m n o b s a m i i
2
Chapter 2 Part Names and Microscopy Procedures (With (With Digital Digital Imaging Head Head Mounted) 2.3 Bright-Field Microscopy
1 . L - b e 1 L - s S t S i h o D n D t e r o h f g n a t c n n m t i i n o o s t i u r i f u a 0 . r d ) e e 9 n n o o p m e i r t h t e i a o s f r h h r e e e s p T W ( p U o l ) o . e n r s o t e U t i . a n b l r o o t r t e o p C n o i n n o s C i a i c t h g n m r n o o i t o r f f s i t a i r d n u e e u p m o r i n s 0 o 9 e i f h r e h ( T e h p t W
t o . n s y r s i i h a s n t s o , e i t n c c i o e e t n s a t r v r o a e l n u s s c b i o n n o i r i o b f o t a p e i h d r T t e e s p f I u o
t o . n y s r s i i h a n t s s o , e i n c t c i o e e t s a n t r v r o a e l n u s s c b i o n n o i r i o b f o t a p e i h d r T t e e s p f u I o
. e 1 L b - s t S i o D h n e t r n h o a t f c t i n m n o o i u r i t f a 0 . r d ) e e 9 n o p m e i o r h t a t o s f r i r e e h e s p T ( p U o
) . e n s o U t i e . a b l r o t t r o n e p n o o n s C i a i c t h n m r o o o i r f t f t a i r d e e n u p m o r i 0 o 9 s f i r e h e T p t h (
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Chapter 2 Part Names and Microscopy Procedures Procedur es (With Digital Imaging Head Mounted) 2.3 Bright-Field Microscopy
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l o : n r s a t e c n u n o l o i a i C v t i t d g n . e n i s o s ] e c u l y i l l n y f a o b o r i u t P n a d [ a v e r r e m e t ] e s s g n v b i g t i a o e s e r t e o h e S T T b [
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Chapter 2 Part Names and Microscopy Procedures Procedur es (With Digital Imaging Head Mounted) 2.4
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Chapter 2 Part Names and Microscopy Procedures Procedur es (With Digital Imaging Head Mounted) 2.4
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Chapter 2 Part Names and Microscopy Procedures Procedur es (With Digital Imaging Head Mounted) 2.4
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Chapter 2 Part Names and Microscopy Procedures Procedur es (With Digital Imaging Head Mounted) 2.4
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. r a b l l o r c S ⇒
k c i l C
l o : n r s a e c t n u n o l o C a i v t i i t d g n . e n i s o s ] e c u l y i l l n y f a o b o r i u t P n a d [ a v e r r e m e t ] e s s g n v b i g t i a o e s e r t e o h e S T T b [
. e 1 L b - s t S i o D h n e t r n h o a t f c t i n m n o o i u r i t f a 0 . r d ) e e 9 n o p m e i o r h t o t a s f r i r e e h e s p T p U o (
: o s t e ] e u l l h e t a f i f . v s o n t o r e P s i o s [ g t i n y i d l l ] t n a g t o u n e c s i n t a t e n o e h i m t S t [ r a e e v v t t r a c i s l e s e s g o e b T S e r o
) . e n s o U t i e . a b l r o t t r o n e p n o o n s C i a i c t h n m r o o o i r f t f t a i r d e e n u p m o r i 0 o 9 s f i r e h e T p t h (
m g a r h p a i d e r u t r e p a c i p o c s i p E
d n n o a n i s o r n e i e m s r m e i c m e m i p m y s i l e l i p h . p t o a e v g , n i t n s e i e e c s v e u i w j t n t c e b e e b o e h j b l i h W o o t •
. e m b i g c e n p r a r a o f o h c c p e s a s i s p . b i e e m g d n e g i n e t r r u h t h a r e t g t h r i r h p n e b t a e i i p e w d c a e e g t e a d h r l e t e t t m p i s u g r u e n m e j i h d p o s T a a C u •
2 1 48
Chapter 2 Part Names and Microscopy Procedures Procedur es (With Digital Imaging Head Mounted) 2.4
1 L S D g n i s u n e h W
. ] o F f B n [ I k C c I i l M c e , h u n t n e O m ) 2 (
. y s ] F a B E [ M k c A i l C c e , u h t n n e O m ) 1 (
l o r t n o C i g n i s u n e h W
e p o c s o r c i m i 0 9
Epi-fluorescence Epi-fluoresc ence Microscopy
l t a a c h i t n t e p e e r r o u l c s o s d e e b e k k m h c o a y t l b M s d r n a . e a s t t s e u r t h a a . c h k s e i c t e p i d l h a p n C t a i p ) 1 (
. ] d l e i F t h g i r B [ k c i l C
E H I D d n a i 0 9 e h t g n i s u n e h W
o d y t l p e o n r i f - c u t s t o h e r r i g i c o r T b m
. h p c t i m a w s l h r c e t t t i u w h s s e h e t h t t s h g s i l e r o P t ) 1 (
e b u d c n r e d a t l i o ) f E h t B o e U n m C . h ( t h n i t h o c w a i t p t i n a l t o i w a i c s t i c x r i t e e s o p p o e v o a e h e t h g t s n r t s a e o e s t r h n n P c i i ) 2 (
s . l e i f d h h i o t e t a b p h e t u p E e e v c l h e t r a m o e h k c i m t t t n c l f o i p o o t f o o l i o t r b . t a e n h e i e t n t t d c r t r e n i o x u r o u a e h t t a e t s n n r h t n e e w i e e i t h v d h m n t m u e o o l e l i l t h l e h t c c i a i a t s a i - t i s t o t s l t p o w o n C a E R s p i •
•
g n i s u t h g i l . c 1 i L p o c S s D a e i d h e t r h t o l n o o r t n n r o u C T i ) 3 (
. ] N O [ k c i l C ) 3 (
f o . . ) ) n k d c o i i e n e t l k o s i r c a t i s o o , o f a l u m C c i n d d ( p ] 6 e n A [ n I m a i D h ( , e 1 ) g h o e u C t n I e t t n o r M p a s o h h e k t O t e ( g i c ] h t n l e 1 r a C [ n u h ) O s c 2 ) ( 1 (
M H I D d n a i 0 9 e h t g n i s u n e h W
e t h h t g r i l o c l i p o r o t c n s o a i C i d g e i n h t s n u . o e 1 c r L n r u S u o T s D •
3 1 49
h . c p t i m w a s l r h e c t i t t u w h s s e e h t h t t s h s i g e r l o P t ) 1 (
. ] A I D [ ⇒
k c i l C ) 2 (
2
. n o t i n r u t o t
k c i l C ) 3 (
e . h g h n t t e a i s d v p u o o l t h a t m c h e e i g t r l . m p i d c 1 n n o i L o a e p i t o h t t c S a t s D i e a e c r r m i x t u o h r d e f e t r e e r e h o h v b t l t o u o e h c n o r t c t r n a t t i t e r n o o w l i u C R s f T i ) ) 2 3 ( (
Chapter 2 Part Names and Microscopy Procedures Procedur es (With Digital Imaging Head Mounted) 2.4
1 L S D g n i s u n e h W
. ] L F y / s F a D E [ M k c A i l C c e , h u t n n e O m ) 1 (
. 1 t L - h g S i D l c e i p h o t r c s o a l i o r d t e n h o t C f f i e o t n a r r u e t p o O t •
o t n i e b u c r e t l i f . h d t e r i a s p e l d a c i a t t r p e o s e n t h I •
t i n r u t o t
k . c i l f f C o ) 1 (
k c i l C ) 2 (
r . d ) h e t o E t l h B i a t f p e U C l n d ( m a r e r i c u i h n t t s c o t p o i i e o t d t w a t s i a e h h c c r t i r t x e t e v e o w . s t p o e i n n s r m h e g i t e a d e t l s n b t a n s h h u a u e c r c c h s t i P e w ) h s 2 t ( e s t s h e f r f P o ) 3 (
o t E - 1 H - . I L t D S h D d e i g n h l a t i c i r p 0 o o 9 l c s o e t r i a h d t n o e g i C h n t i e f s t f u a r o n e r e p n u h O t ) W 1 (
h g u o , r u t h n e ] 1 m [ 1 . f . C ] o I F e F n M r O o o e [ h k f t k i c c ] n i 6 l l C [ O c ) ) 2 1 ( (
. s d s e r r e i s d d e D a ⇒
. ] e c n e c s e r o u l F [ k c i l C ) 1 (
d l e : i f - y e t p h o m g c u i s r s o e b - r r i c o p i T E m
] 6 e [ n h 1 o g k C c u I o i l r . M c t h e , ] h u t 1 n r n e [ f o O m o f ) 3 (
. ] o f L F n [ I k C c I i l M c e , h u t n n e O m ) 2 (
l o r t n o C i g n i s u n e h W
e p o c s o r c i m i 0 9
Epi-fluorescence Epi-fluoresc ence Microscopy
. s d s e r r e i s d d e D a ⇒
k c i l C ) 2 (
M o - t H 1 I L - . D S t h d D g n e i l a h c i t 0 r i p 9 o o c l e o s r i a h t t d n g o e n C h i t s i e f u t f n a r o e e n r h p u O t W ) 1 (
e . h e c t f i o v e r d e n t t i o u t h a s n e i h m t u l n l i e i p p O E •
4 1 50
l a c i t a p d t o r o h e e t s t h e n i o m t d n n i o n i a t t e a b t i e c r r u c x t u r e e r t e e l i h v t f o d e h e t . r h c i a t i s t t o w e a R s d p ) 2 (
d n a o , t ) e s d h e t e s g o t n l a a C h h ( t c e r n u i o ) . s t a n e c e k k i c p i l a d O n ( C m i ) 3 (
l a c i t p o e . h d t e t t c a e h n t . e n r o u c s i s e h k k c t i l a a C M p ) 3 (
f f o n r u t o t h c t i w s r . e p t t m u a l h s h c e t h i t s w s s e e r h P t ) 3 (
Chapter 2 Part Names and Microscopy Procedures Procedur es (With Digital Imaging Head Mounted) 2.4
Epi-fluorescence Epi-fluoresc ence Microscopy
1 . L - e 1 L S t h S D D f o e e h g i d t n s n i " o s ○ h c u " t i e w n t h s r e s e s w h e r o W P p
2
l o f r f o t n t r n r u o o p t p . C u d i S n C P a s , e g i e e h n e r t r a i f s i w o S t r u " f o e s e w n t h " o s p l e o e h d n o h E T t W o t t e i h n t r f u o t h e c r t i t o w s t i . r n n e u o i t w y i o l s p p p o e u p h s F t r F s e O s w e e r o h P p t
e p o c s o r c i m i 0 9
r e w o . p s e r e g n h o t n i t f i t f f a t e a v o h l r c p e t n r i m s u w o b T s c o
5 1 51
Chapter 2 Part Names and Microscopy Procedures Procedur es (With Digital Imaging Head Mounted) 2.5 Photomicroscopy
y p o c s o r c i m o t o h P
e h T . s t c u d o r p e v i t c e p s e r e h t h t i w d e d i v o r p s l a u n a m g n i t a r e . p t o i e n u h l t o o t r t r n o e c f e r a , r C e P m d a n c a 1 , L e r S a D w d t f o n s a c i M p 5 o c S s D o r c d i a e m o h t o a r h e p m , a a r c e S m D a a c e e h m t f u o s s s n a o s i s n o s t i u c c u s r i d t s d i e n l i g a n t e i d w o r l o l o F f
5 . 2
52
Chapter 2 Part Names and Microscopy Procedures Procedur es (With Digital Imaging Head Mounted) 2.5 Photomicroscopy
1 L S D g n i s u n e h W
.
k c i l C
l o r t n o C i g n i s u n e h W
. ] P r a e R [ ⇒ k c i l C
e p o c s e o r g c a i . m n m i o e r i e t h t p a t o r s r v p u j r e s d o b A f o
1
s . u " o r y i p v o o e r " c s p y p o r n o i c i c n s M e o r e v c i c g i n s M e n l d c s o e e i i r t c F o - u e r t l i h f d i g i e r p h B E t e 3 . 4 . e 2 2 S " "
) . e n s o U i e . t a b l r o t t r e o n p n o o n s C i a i c t h n m r o o o i r f t f t a r d i e e n u p m o r i 0 o 9 s f i r e h e T p t h (
s r . .
E H I D d n a i 0 9 e h t g n i s u n e h W
e p o c s o r c i m i 0 9
. e 1 L b - s t S i o D h n e t r n h o a t f c t i n m n o o i u r i t f a . r d 0 ) e e 9 n o p m e i o r h t o t a s f r i r e e h e s p T p U o (
. h P t . a R p h c e l t h . a i t s c i w t t s t h p r a g h o e t i l e v r o o o s h e t t g ) a s n c H s a I i e d r h D n P c ( i
. P . R
) e ) M 1 2 - v ( e r ( H l r I g e e D i v n v e e d h l l r r c e n t a i e i w p p w 0 s u o l 9 t h e e e a t h h h p t t t l n u i g a c o i h n l i t s s p l u u u O P P n e h e W h
t h d t r a a p w l o a t t c r i d o t e p t o c p r l l e a r e i u F d r
h . t t a r p l o a p c r i t a p e r o e e h t h t d t r c a e r w i o D t
2 53
2
Chapter 2 Part Names and Microscopy Procedures Procedur es (With Digital Imaging Head Mounted) 2.5 Photomicroscopy
1 L S D g n i s u n e h W l o r t n o C i g n i s u n e h W
e p o c s o r c i m i 0 9
. e 1 L b - s t S i o D h n e t r n h o a t f c t i n m n o o i u r t f i a . r d 0 ) e e 9 n o p m e i o r h t o t a s f r i r e e h e s p T p U o (
t s a u l e o : . y t n e , a o g i r t a 1 e r L a o - t t m S n e r s D d o a e n f n t a i h a t g d e r g s n o n o i f i s o w o r u h l e c l d o l n t f e s o e h u F h W m t •
) . e n s o U i e . t a b l r o t t r e o n p n o o n i C s a i h c t n m r o o o i r f t f t a i r d e e n u p m o r i 0 o 9 s f i r e h e T p t h (
e s U e . b l o t t r s o n i h n o t n C r a i o c f n m t i o o i r n t f u a . r d ) n e e 1 L o p m - i o r S t o D a s f r i r e e h e p T p t h o (
) B (
) A (
d a r e e e h y t l a m g l n a i i p c h t i s n c e a u d . h s l y t t n i t t o e r a i s d t g e i u j a s a p d e o m o r A h p i p
. d ) e " . v o a t s u e A b " o t c t e e l l i e s f f n o a e c m u o a Y N ( •
1 L S D e h t e b e s ) t U . o n . i n i o n 0 t a 9 a r c n m e p o o i r o t f s a r d i h e e t p m r o r o o f s f i t h r i n T e ( p u
) B ( d n a ) A ( g n r i e t s t p w u j a e r d d c A a s t g n n i u n o i a m t e C r
e n e h o t g n e a e g g t t e i n a s r h h g v m i n e t e e t t o i t o t s i , e t p h s m a u t h s d d o g t t e a j d n a s p s n e a t h e p i a t d v o k m b n n o n s o e a t s t o u . a h m u y m t n i o n t j l r m w e t o h i n t e d c e t i m a r r i i e C c g r f m r i t s f e r A i e o t s d h s o t - i . a w u t g p f t n i j n r e e n n i n a l o i o r d o p c r t n e e i o e A i m s c r s a t g e ) o o t m a e i e r p p h o e a t h 1 ( m L r c s t d a t
3
. l a s u n g i s a h m n t i t g . o i t f n a t r n e i o a r e s s m s e p a u o c a c r i s e e h h t e d t d h o m l e t t i i r a t a f e w c e d e r e d e i , d c i k a r p v o r o o a F t p M
4 54
. e f z o i s n o i e t l i f a n i d t n s a e t d a m d n r a o f e a t e t l i a a F D d •
•
Chapter 2 Part Names and Microscopy Procedures Procedur es (With Digital Imaging Head Mounted) 2.5 Photomicroscopy
1 L S D g n i s u n e h W l o r t n o C i g n i s u n e h W
e p o c s o r c i m i 0 9
e e e d t h h o t M n i f e o n n e w u . c o n S h e u n e e s m h m t n o y e t t s M s u a A U b E C
t e g e r n e B g a M n t r c i e n i W n o i A e h C l , n t r c m e f c y a h e e u i w c s h l g b h t a i t p a e e r p b l e a p o a l s h s l r e a s t c f u g t e t s r l e o a f o i m f u r r h r s ) j p o i e f . h c o o n g d , g o F i w n o n a e w a n ( i m i c n r t i t o . t o t m s e n o u i i d o e o j t u a t t n d l n o c d o a u e n e i h l h d n o T b b m a s s p a u c s
e s U e l . b o t t r i s o h n n t n o r C a i o c f n m t i o o i r n t f u a . r d 1 ) e e L n o p m - i o r S t o D a s f r i r e e h e p T p t h o (
e s U e l . b o t t r i s o h n n t n o r C a i o c f n m t i o o i r n t f u a . r d 1 ) e e L n o p m - i o r S t o D a s f r i r e e h e p T p t h o (
1 L S D e h t e b e s ) t U . o n . i n i o n 0 t a 9 a r c n m e p o o i r o t f s a r d i h e e t p m r o r o o f s f i t h r i n T e ( p u
1 L S D e h t e b e s ) t U . o n . i n i o n 0 t a 9 a r c n m e p o o i r o t f s a r d i h e e t p m r o r o o f s f i t h r i n T e ( p u
e l b y a a p r t e i o c s m u s a e o c r c i e d o . h t m m d e o t e h c n t h e e t l e e c r o S s f m
5
e t i h w a r e m a c . e e c h t n a t l e a S b
6 55
2
Chapter 2 Part Names and Microscopy Procedures Procedur es (With Digital Imaging Head Mounted) 2.5 Photomicroscopy
1 L S D g n i s u n e h W l o r t n o C i g n i s u n e h W
e p o c s o r c i m i 0 9
g s i n r i , e e ) s e f e . o h l u d e t e b t r l a o . g s r r a n , s n n u d c a e t o e e i o i d m p t e i u m r n t s t e e l i p n a e t u c u u c c n a t o a c h s n c b o e t g e r e o a c . i u g . s m e p s d i r p f g h . i s T e h b x n a n P g g L a t i n t a e e i m F o i o A h n / r f t t i t e g a t g C m t o o a F F r a e t r i a e a e i a u s t r D . n e w h b m e e m m n h h o p d i f i t i t a e k e t p i d s o e e s c p c z e s e o s s e i d r t h s u c u h e e t j v v e f e l e e m h e c e t r r d h f o o a h f s r e i T i A t c C F I p s ( o d s d t p ・ ・ ・
d e t c u . d n n e o c m i s c i . e 1 p e r L s u t S e p D h t . a e n s c h o u t i c e t g i f s o a m o e m o r P R I f ・ ・ ・
m o r f d e t c u . d n n e o c m i s c i e p e r s u t . e p 1 h t . a L c n s o u e i c g S t D i f s o a e o e m h P R I t ・ ・ ・
e v a s d n a . e r s u e t g p a a m C i
7 56
3
Assembly
57
Chapter 3 Assembly
58
Chapter 3 Assembly
3
59
Chapter 3 Assembly
List of cables 1
Control cable
2
Lamp cable
3
USB cable
4
DIH control cable
Provided with the DIH
5
USB cable
Sold separately
6
Camera cable
Sold separately
Provided with the 90i
PC
DIH 4
3
90i
5 Motorized ND filter
Motorized nosepiece
Camera control unit DS-U1
Lamphouse
1
6
2
Power supply unit
AC power outlets
60
Camera head DS-5M
Chapter 3 Assembly
1
Checking the Input Voltage Check the input voltage indicated on the back side of the power supply unit to make sure that it complies to the local power supply voltage. Note: If the voltage indication and supply voltage
Input voltage indication
differ, do not attempt to use the microscope . Contact your nearest Nikon representative to seek advice.
Back side of the power supply supply unit
2
Installing a Lamp (1)
Lamphouse-cover fixing screw
Loosen the lamphouse-cover fixing screw and lift the lamphouse cover to remove.
(2)
Attach the lamp to the lamp plug. Avoid touching the bare lamp bulb with your bare hands. Use the lamp type specified. Using an unspecified lamp may cause malfunction or fire. Refer to Chapter 8 for the specified lamp.
(3)
Replace the cover and tighten the screw.
Lamp plug
CAUTION The lamphouse cover must be attached. Failure to replace the lamphouse cover may result in burns or fire from the heat generated by the lamp.
61
3
Chapter 3 Assembly
3
Preparing for connector connection Open the cover on the back side of the microscope. To open the cover, disengage the two hooks on the right side first. Connectors are located inside.
Cover
Disengaging the two hooks to open the cover.
Inside of the cover
4
Motorized ND filter
When using the motorized ND filter
Fixing screw (1)
Attach the motorized ND filter to the lamphouse mounting section on the back side of the microscope, and tighten the fixing screw to secure it.
Lamphouse mounting section
(2)
Connect the connector to the ND connector located inside the microscope.
⇒
Refer to the connection diagram on page 60.
ND connector
Back side of the 90i
62
Chapter 3 Assembly
5
Installing the lamphouse (1)
Motorized ND filter
Attach the lamphouse to the back side of the Lamphouse
motorized ND filter, and tighten the fixing screw to secure it in place. (When the motorized ND filter is not used, install the lamphouse to the lamphouse mounting section of the microscope.) (2)
3
Connect the lamp cable between the lamphouse connector and the round connector on the back of the power supply unit (LAMP).
⇒
Fixing screw
Refer to the connection diagram on page 60.
Lamp cable
Back side of the the power supply supply unit
6
When Directly Attaching an Eyepiece Tube (1)
Place an eyepiece tube on the microscope arm. Secure in place with a tool stored in the back of the microscope.
(2)
Attach the eyepiece by aligning the projection of the eyepiece tube sleeve with the notch on the eyepiece.
(3)
Attach a screw hole protection protectio n sticker provided with the microscope to the bolt hole found on
Fixing the eyepiece tube
the top surface of the arm of the microscope. Projection
Notch
Eyepiece attached in place
63
Chapter 3 Assembly
7
When using the digital imaging head (1)
Place the DIH-E or DIH-M (hereafter referred to
Cover fixing screw
as the "DIH") on the microscope, and secure it using the screw located on the front of the arm. (2)
Remove the back cover of the DIH-M by loosening the two screws.
(3)
Fix the DIH to the microscope with two screws inside.
Screws (3 locations) used for DIH installation (4)
Connect the ferrite core side of the DIH control cable to the CTL connector. (The DIH control cable and the USB cable are DIH Control cable
provided with the DIH-M.) (5)
Replace the cover.
Interlocking connector for C1 (used only for connection of C1)
Ferrite core
For maintenance (not used)
(6)
Release the fixing screws on the DIH-M. 1)
Remove the cover on the left side of the DIH-M.
2)
Viewed from the other side
Loosen the optical path switching switching section
Optical path switching section fixing screws
fixing board mount screw. 3)
Loosen the two two optical optical path switching switching section fixing screws to release them from the inside optical path switching section.
4)
Move the the fixing fixing board slightly forward with with the two fixing screws attached, then tighten the mount screw.
5)
Replace the cover.
6)
Loosen and remove the zooming section
Fixing board for optical path switching section
Cover
Optical path switching section fixing board
Blind cap
fixing screw from the DIH. 7)
Attach the blind cap provided.
Zooming section fixing screw
64
Chapter 3 Assembly
(7)
Fold and break off the section (see the diagram
Fold and break off this section.
on the right) on the back of the microscope to make an opening for the DIH control cable.
3 (8)
Connect the DIH control cable to the DIH connector on the back side (inside) of the microscope. DIH connector
Refer to the connection diagram on page 60.
(9)
Attach the mercury lamphouse to the bayonet
Back side of the 90i
mount on the back. (For more information, Bayonet mount
refer to the instruction manual provided with the mercury lamp power supply.) 1)
Attach a collector lens to the lamphouse.
2)
Turn the bayonet mount clockwise (viewed from the back) as far as it will go.
3)
Insert the lamphouse into position.
4)
Turn the bayonet mount anticlockwise (viewed from the back) to lock the lamphouse.
Mercury lamphouse
Eyepiece
5)
Attach a mercury lamp.
6)
Connect the mercury lamphouse to the mercury lamp power supply.
(10) Attach the eyepiece by aligning the projection of the eyepiece tube sleeve with the notch on the eyepiece. Projection
Notch
Eyepiece attached in place
65
Chapter 3 Assembly
8
Attaching the motorized nosepiece (1)
Put the cable of the motorized nosepiece through the hole in the microscope body.
(2)
Lift the nosepiece from a position just forward
Put the cable through the hole.
of the point directly below the fitting part and slide toward the back to attach. (Continue
Fixing the nosepiece
sliding the nosepiece until its front position is aligned with that of the fitting part.) (3)
Tighten the screw.
(4)
Locate the cable of the motorized nosepiece routed through the hole on the back side of the microscope, and connect it to the REV connector. Refer to the connection diagram on page 60. REV connector
Back side of the 90i
66
Chapter 3 Assembly
9
Attaching a Camera (when using a camera) 9-1 When attaching to the ergonomic binocular tube: (1)
Camera head
To attach a camera head, screw it into the C mount on the DSC port.
(2)
Remove the rear cover of the ergonomic
3
binocular tube and insert the DSC port. (3)
Secure the DSC port into place with a screw.
DSC port fixing screw
(4)
DSC port Fixing the camera cable
Attach the camera cable to the camera head. (Adjust the camera head position before using the camera.)
Mounting direction fixing screw Camera centering screws
9-2 When attaching the DIH: (1)
Camera fine focus adjustment screw
C mount mount adapte adapterr
Attach the camera head to the rear port of the DIH-M using the C mount adapter.
(2)
Attach the camera cable to the camera head. (Adjust the camera head position before using the camera.)
C mount adapter retaining screw
67
Chapter 3 Assembly
Removing the elevating section 10 fixing screw.
Fixing screw
Remove the fixing screw from the stage elevating section.
11 Connecting the power supply unit (1)
Make sure that the power switch of the power supply unit is turned off (button in projected position).
(2)
Connect one end of the the control cable to the the back side of the power supply unit, and the other end to the POWER connecter on the back side (inside) of the microscope.
(3)
Connect one end of the power cord to the AC inlet on the back panel of the power supply unit, and the other end to an AC outlet.
Make sure that the power switch is turned off.
Be sure to use the specified power cord. Refer to Chapter 7 for the specified power cord. (4)
Check each connector for secure connection. Refer to the connection diagram on page 60.
Use the control cable to connect the POWER connector on the back side of the 90i. Use the lamp cable to connect to the lamphouse. Use the specified power cord to connect to an AC outlet. Back side of the power supply unit
Power connector Back side of the 90i
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Chapter 3 Assembly
12 Attaching a condenser 12-1 When using the motorized universal condenser (1)
Remove the retaining screw of the motorized universal condenser.
(2)
3
Before attaching the motorized universal condenser, mount modules such as DIC prisms on the turret of the co ndenser.
⇒
Retaining screw
Refer to the instruction manual provided with the motorized universal condenser.
(3)
Make sure that the fixing screw has been removed.
After confirming confirming that the fixing screw has been removed from the stage elevating section, turn on the power switch (button in depressed position) of the power supply unit. When the power switch is turned on, the LED located above the switch will light and a long buzz will sound. Initialization will then begin. When initialization is complete, the buzzer will sound once again.
CAUTION Before turning on the power, be sure to check that the fixing screw has been removed from the stage elevating section. If the power switch is turned on with the fixing screw attached, a malfunction may result. (4)
After initialization initialization is is complete, complete, turn the focus knobs and raise the stage to its uppermost position.
(5)
Press the power switch of the power supply unit
Power switch
to return it to the OFF position (button in projected position). (6)
Place the motorized universal condenser on the condenser mounting section, and insert the pin
Fixing screw
Guide hole
Motorized universal condenser
into the guide hole of the stage elevating section.
CAUTION Before attaching the motorized universal condenser, condenser, make sure that the power is turned off. If the motorized universal condenser is installed while the power is on, a malfunction may result. (7)
Condenser mounting section
Tighten the fixing screw.
69
Pin
Chapter 3 Assembly
12-2 When using a condenser of other type (1)
Guide hole Pin
Place the condenser holder on the condenser mounting section, and insert the pin into the
Condenser focus knob
guide hole of the stage elevating section. (2)
Tighten the fixing screw.
(3)
Turn the condenser focus knob and lower the elevating section of the condenser holder to the lowermost position.
(4)
Insert the condenser, face it forward, then tighten the screw to secure it in place.
(5)
Turn the condenser focus knob and raise the condenser to its uppermost position.
70
Secure condenser in place.
Chapter 3 Assembly
13 Attaching a Stage (1)
Turn on the power switch (button in depressed position) of the power supply unit. When the power switch is turned on, the LED located above the switch will light and a long buzz will sound. Initialization will then begin. When initialization is complete, the buzzer will sound
3
once again.
Press the power switch to the ON position. Stage fixing screws (2)
After the initialization is complete, place the stage on the stage elevating section, and secure it using the screws. (For the stage with a centering function, place the stage on the elevating section by aligning
Groove
the projection on the bottom of the stage with the groove of the elevating section. There are two screws to fix the stage with a centering function.)
Projection
Bottom of the stage with centering centering function
(3)
Place a specimen holder on the stage and tighten screws.
(4)
Fixing the stage with centering function
Turn the the focus focus knobs knobs and lower the stage to its lowermost position.
Fixing the specimen holder
71
Chapter 3 Assembly
14 Attaching Objectives Screw objectives into the motorized nosepiece. Be sure to attach objectives to the nosepiece in the order of the lowest magnification to the highest magnification from Position 1.
Screw objectives into the nosepiece.
Filter Cubes and attaching a Light Shield 15 Inserting (When DIH is installed) (1)
There is a front cover below the eyepiece. Pull out the cover to remove.
(2)
Address indication
Excitation method switchover turret
Insert a filter cube into position. For the DIH-E, leave the address 6 empty. (When using a PC or DS-L1 to switch to bright-field microscopy, the address 6 will be automatically automatically brought into the optical path.)
Filter cube bay
Address indication
Filter cube
(3)
Insert a nameplate into the position with the same address found in the filer cube bay.
(4)
Press the excitation method switch (in the case of the DIH-M, manually turn the excitation method switchover turret), and install filter cubes and nameplates in other positions.
(5)
Replace the cover.
Nameplate
(6)
Screw a light shield onto the lower front side of the DIH.
Light shield
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Chapter 3 Assembly
16 Connecting a PC (1)
Install the "i Series Series Support Tools" software in the PC before connecting it to the microscope.
⇒
Regarding the software installation method, refer to the manual for the "i Series Support Tools" software.
(2)
Connect the USB cable provided with the microscope to the USB connector on the microscope and the USB connector on the PC.
(3)
Reinstall the cover cover on the back side side of the microscope.
Assembly is now complete. Make settings by referring to the manual for the "i Series Support Tools" software.
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3
4 4.1
Replacing Consumables Replacing the Lamp CAUTION • Be careful to avoid burns:
Wait until the lamp and nearby parts have cooled before attempting to replace the lamp. • Be careful to avoid electrical shock:
Turn off the power switch and unplug the power cord from the wall outlet.
4
• Be careful to avoid abnormal heat generation:
Use only the lamp specified. • Be careful to avoid actions that might reduce lamp service life:
Avoid touching the bare lamp bulb with bare hands. Soiling will reduce the service life of the lamp.
(1)
Loosen the screw and lift the cover to Lamphouse cover fixing screw
remove. (2)
Remove the old lamp.
(3)
Replace with a new lamp. Avoid touching the bare lamp bulb with bare hands. Use only the lamp specified. Refer to chapter 8 for the specified lamp.
(4)
Replace the cover and fix it with the screw.
Lamp plug
CAUTION The lamphouse cover must be attached. Failure to replace the lamphouse cover may result in burns or fire from the heat generated by the lamp.
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5
Troubleshooting If the product does not function property, take appropriate action as described below. If the problem is still not r esolved after referring to "Troubleshooting," please contact your nearest Nikon representative.
5.1
Optical Problem
Field of view vignetts.
Possible causes Parts are attached incorrectly.
Attach the parts (nosepiece, (nosepiece, condenser, etc.) correctly.
Movable parts are not switched correctly.
Move the parts (optical path switching lever, excitation method switchover turret, filter slider, condenser turret, etc.) correctly. (Position the parts until a click is felt.)
Condenser is adjusted incorrectly. incorrectly.
Adjust the condenser so that the field diaphragm image is in focus at the center of the field of view.
The objective-condenser combination is incorrect.
Use a proper combination.
Lamp is attached incorrectly.
Attach the lamp securely and adjust it if necessary.
Field diaphragm is not sufficiently sufficiently opened.
Open the field diaphragm to the proper size.
Dirt or dust is p resent on the lens, condenser, eyepiece, filter or specimen.
Clean as appropriate.
Field diaphragm image is not focused on the specimen surface.
Focus and center the condenser.
Aperture diaphragm is stopped down too far.
Open the aperture diaphragm to the proper setting.
Aperture diaphragm is stopped down or opened too far.
Open or stop down the aperture diaphragm to the proper setting.
Objective correction ring is not adjusted.
Adjust the correction ring to match the cover glass.
Condenser is incorrectly adjusted.
Adjust the condenser so that the field diaphragm image is in focus at the center of the field of view.
Illumination is uneven across the field of view. Field of view is not visible.
Dirt or dust is present in the field of view.
Cover glass is unattached. Image quality is poor. Contrast is poor. Resolution is poor.
Remedy
Cover glass thickness is not as specified. The tip of the oil-immersion objective objective is not immersed in oil.
Attach a cover glass of the specified thickness (0.17 mm).
Use Nikon immersion oil.
Unspecified immersion immersion oil is used. Air bubbles are present in the immersion oil.
Remove the air bubbles.
Immersion oil is attached to the tip of the dry objective.
Clean it off.
Dirt or dust is pr esent on the lens, condenser, objective, or specimen. specimen.
Clean as appropriate.
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Chapter 5 Troubleshooting Troubleshooting 5.1 Optical
Problem
Focus is uneven.
Image flows.
Image is tinged yellow. Field of view is too bright.
Possible causes
Remedy
Nosepiece is not attached correctly, or not rotated to the click stop position.
Attach it correctly and make sure that it is rotated to the click stop position.
Specimen is tilted relative to stage surface.
Securely fit the specimen onto the stage specimen holder.
Nosepiece is not attached correctly, or not rotated to the click stop position.
Attach it correctly and make sure that it is rotated to the click stop position.
Specimen is tilted relative to stage surface.
Securely fit the specimen onto the stage specimen holder.
Condenser is tilted.
Attach it correctly.
NCB11 filter is not being used.
Use the NCB11 filter.
Lamp voltage is too low. Lamp voltage is too high.
Press the preset switch and adjust the brightness with the ND filters.
Lamp voltage is too low.
Field of view is too dark.
Focus is not possible with a high-magnification objective.
Condenser aperture diaphragm is stopped down too far.
Open the aperture diaphragm to the proper setting.
Condenser is incorrectly adjusted.
Adjust the condenser so that the field diaphragm image is in focus at the center of the field of view.
Image optical path is distributed to several positions.
Adjust the optical path switching lever to provide 100% illumination for the target.
Specimen is upside down.
Attach the specimen to the stage with the cover glass facing up.
Cover glass thickness is not as specified.
Attach a cover glass of the specified thickness (0.17 mm).
Diopter is not adjusted. There is too great a difference in focus after switching the objective.
Diopter is not adjusted.
Specimen does not move smoothly.
Specimen holder is not securely attached to stage.
Attach the specimen holder securely to the stage.
Images on the left and right eyepiece are not coincident.
Interpupillary distance is incorrect.
Make adjustments. adjustments.
Diopter is not adjusted.
Adjust the diopter.
Adjust the diopter.
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Chapter 5 Troubleshooting Troubleshooting 5.2 Electrical
5.2
Electrical
Power on
Problem There is no power even though the power switch is turned on.
When power is turned on, a series of short beeps sound, and the escape switch lamp flashes.
Possible causes Power cord is not connected, or is connected improperly.
Vertical motion counter is malfunctioning.
Remedy Connect it properly. Refer to "11 Connecting the power supply unit" in Chapter 3 "Assembly. " Press the escape switch and initialize the vertical motion. After the initialization is complete, the buzzer emits a long buzz. Note: The buzzer emits short beeps for 20 seconds. If the escape switch is not pressed during this time, the vertical motion will be initialized initialized automatically.
Lamp Problem
Possible causes
Remedy Replace the lamp with a specified one.
Lamp has burned out.
Refer to "2 Installing a Lamp" in Chapter 3 "Assembly."
Lamp does not light.
Attach the specified lamp. Lamp is not attached.
Lamp burns out quickly.
Microscope brightness control knob is not functional.
Refer to "2 Installing a Lamp" in Chapter 3 "Assembly."
Lamp used is unspecified/incompatible.
Replace the lamp with a specified one. Refer to "2 Installing a Lamp" in Chapter 3 "Assembly." Turn off the preset switch. Refer to Refer to "1.2.2 Left view in Chapter 1 "Part Names and Microscopy Procedures."
Preset switch is on.
Vertical motion Problem When objectives are switched, vertical motion does not operate in interlocking mode. Interlock setting and parfocal correction are ON, but focus adjustment is not conducted or the image appears out of focus when objectives are switched.
Possible causes
Remedy Change the settings to ON.
Interlock function is disabled, or parfocal correction is set to Off.
Refer to "8.2 Canceling the automatic adjustment of motorized attachments when switching objectives" and "10 Focus Adjustment" in the reference manual.
Set the focus position of each objective Focus positions of objectives are incorrectly set.
correctly. Refer to "10 Focus Adjustment" in the reference manual.
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5
Chapter 5 Troubleshooting Troubleshooting 5.2 Electrical
Problem
Possible causes
Remedy Change the vertical motion range.
Focus cannot be adjusted by turning focus knobs.
Focal point is located outside of the vertical motion range.
Refer to "5.4 Changing the vertical stage motion range and its lower limit" in the reference manual.
Focus knob mode cannot be switched between coarse, fine or extra-fine.
Set the operation mode to Manual. Operation mode is set to Auto.
Refer to "5.3 Number of knob turns and distance of stage travel" in the reference manual. The lower limit of the vertical motion range
Pressing the escape switch does not move the stage elevating section to the escape position.
can be changed when using a condenser other than the motorized universal Escape position is outside the lower limit of the vertical motion range.
condenser. Refer to "5.4 Changing the vertical stage motion range and its lower limit" in the reference manual.
Motorized nosepiece Problem
Possible causes
Remedy Connect the cable correctly.
Motorized nosepiece does not move at all.
Cable of motorized nosepiece is not Refer to "8. Attaching the motorized
connected.
nosepiece" in Chapter 3 "Assembly. " Objectives cannot be changed smoothly.
The objectives are mounted only on
Press the objective changeover switch to
one side of the nosepiece.
rotate the nosepiece several times. Press the objective changeover switch
Buzzer sounds twice when the motorized nosepiece is rotated, and the power indicator lights up twice in red.
again. Control error has been generated.
If a control error is still generated after repeated pressing of the objective changeover switch, turn off the power and turn it on again. Rotate the nosepiece in the reverse direction or cancel the limiter for high
High magnification objective sometimes does not enter optical path.
magnification magnification objectives. Limiter is activated for high magnification magnification objectives.
Refer to "9.1 Prevention contact between the objective and specimen when switching objectives" in the reference manual. Cancel the setting.
Positions without objectives are skipped.
Setting has been made to skip positions not mounted with objectives.
Refer to "9.2 Skipping positions not mounted with an objective" in the reference manual.
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Chapter 5 Troubleshooting Troubleshooting 5.2 Electrical
Problem
Possible causes
Remedy Set the information for mounted
Positions whether mounted with or without objectives are skipped, and certain objectives cannot be used.
objectives correctly. The settings for mounted objectives are incorrect.
Refer to "2.1 Setting the information of mounted objectives, filter cubes, and condenser modules" in the reference manual.
The objective cannot be switched to a liquid immersion objective, and a liquid immersion objective cannot be switched to a dry objective.
A setting has been made to prohibit switchover to a liquid immersion objective and from a liquid immersion objective to a dry objective.
Cancel the setting. Refer to "9.3 Preventing the adhesion of oil/water to dry objectives" in the reference manual.
Set the information for mounted objectives correctly. A dry objective cannot be switched to another dry objective.
Settings for mounted objectives are incorrect.
Refer to "2.1 Setting the information of mounted objectives, filter cubes, and condenser modules" in the reference manual.
Stage elevating section moves to the escape position when an objective is switched to a liquid immersion objective or when a liquid immersion objective is switched to a dry objective.
A setting has been made to move the stage elevating section to the escape position when an objective is switched to a liquid immersion objective or when a liquid immersion objective is switched to a dry objective.
Cancel the setting. Refer to "9.3 Preventing the adhesion of oil/water to dry objectives" in the reference manual.
Set the information for mounted The stage elevating section moves to the escape position when a dry objective is switched to another dry objective.
objectives correctly. Settings for mounted objectives are incorrect.
Refer to "2.1 Setting the information of mounted objectives, filter cubes, and condenser modules" in the reference manual.
Motorized universal condenser Problem
Possible causes
Remedy Install the device correctly.
The turret and aperture diaphragm of motorized universal condenser do not move at all.
The motorized universal condenser is not installed correctly.
The center of a module such as DIC prism deviates from optical axis.
A control error has been generated, or a turret was moved by hand while the unit was ON.
Refer to "12-1 When using the motorized universal condenser" in Chapter 3 "Assembly." Turn off the power switch and turn it on again. Do not move the turret by hand while the power switch is ON.
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5
Chapter 5 Troubleshooting Troubleshooting 5.2 Electrical
Problem When objectives are switched, the turret of a motorized universal condenser will not operate in interlocking mode. When objectives are switched, the aperture diaphragm of a motorized universal condenser does not operate in interlocking mode. When objectives are switched, the DIC prism (or another module) corresponding to the objective does not enter the optical path. When objectives are switched, the aperture diaphragm does not adjust to an appropriate size. The settings for mounted objectives are correct, but the aperture diaphragm does not adjust to an appropriate size when objectives are switched.
Possible causes
Remedy Set the setting to ON. Refer to "8.2 Canceling the automatic
Interlock function is disabled.
adjustment of motorized attachments when switching objectives" in the reference manual. Change the setting to ON. Refer to "8.2 Canceling the automatic
Interlock function is disabled.
adjustment of motorized attachments when switching objectives" in the reference manual. Correct the settings.
The settings for mounted objectives and modules (such as DIC prisms) are incorrect.
Refer to "2.1 Setting the information of mounted objectives, filter cubes, and condenser modules" in the reference manual. Correct the settings.
The settings for mounted objectives are incorrect.
Refer to "2.1 Setting the information of mounted objectives, filter cubes, and condenser modules" in the reference manual. Return the correcti correction on value to its default or
An inappropriate value has been set for correction of the aperture diaphragm size in the switching of objectives.
set an appropriate value. Refer to "8.4 Changing the size of the aperture diaphragm when switching objectives" in the reference manual.
Field diaphragm Problem When objectives are switched, the field diaphragm does not operate in interlocking mode.
When objectives are switched, the field diaphragm does not adjust to an appropriate size.
Possible causes
Remedy Change the setting to ON. Refer to "8.2 Canceling the automatic
Interlock function is disabled.
adjustment of motorized attachments when switching objectives" in the reference manual. Correct the settings.
The settings for mounted objectives are incorrect.
Refer to "2.1 Setting the information of mounted objectives, filter cubes, and condenser modules" in the reference manual.
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Chapter 5 Troubleshooting Troubleshooting 5.2 Electrical
Problem The settings for mounted objectives are correct, but the field diaphragm does not adjust to an appropriate size when objectives are switched.
Possible causes
Remedy Return the correction value to its default,
An inappropriate value has been set for the correction of field diaphragm size in the switching of objectives.
or set an appropriate value. Refer to "8.3 Changing the size of the diascopic field diaphragm when switching objectives" in the reference manual.
Motorized ND filter Problem
Possible causes
Motorized ND filter does not operate at all.
The cable of the motorized ND filter is disconnected.
Remedy Connect the cable securely. Refer to "4. When using the motorized ND filter" in Chapter 3 "Assembly."
When objectives are switched, the motorized ND filter does not operate in interlocking mode.
Change the setting to ON. Refer to "8.2 Canceling the automatic Interlock function is disabled.
adjustment of motorized attachments when switching objectives" in the reference manual. Correct the settings.
When objectives are switched, optimum brightness is not achieved.
The settings for mounted objectives are incorrect.
Refer to "2.1 Setting the information of mounted objectives, filter cubes, and condenser modules" in the reference manual.
Settings for mounted objectives are correctly set, but optimum brightness is not achieved when objectives are switched.
Return the correction value to default, or set an appropriate value. Inappropriate value has been set for correction of field diaphragm size in switching of objectives.
Refer to "8.5 Changing the amount of adjustment of the motorized ND filter when switching objectives" in the reference manual.
Digital imaging head Problem
Possible causes
Remedy Connect the cable correctly.
Optical path does not switch.
Cable is not connected correctly. correctly.
Refer to "7 When Using the Digital Imaging Head" in Chapter 3 "Assembly."
Episcopic field diaphragm does not move.
Connect the cable correctly. Cable is not connected correctly. correctly.
Refer to "7 When Using the Digital Imaging Head" in Chapter 3 "Assembly." Connect the cable correctly.
Filter cubes do not switch.
Cable is not connected correctly. correctly.
Refer to "7. When Using the Digital Imaging Head" in Chapter 3 "Assembly."
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5
Chapter 5 Troubleshooting Troubleshooting 5.2 Electrical
Problem
Possible causes
Remedy Connect the cable correctly.
Shutter does not operate.
Cable is not connected correctly. correctly.
Refer to "7 When Using the Digital Imaging Head" in Chapter 3 "Assembly." Connect the cable correctly.
Analyzer does not operate.
Cable is not connected correctly. correctly.
Refer to "7. When Using the Digital Imaging Head" in Chapter 3 "Assembly."
When an optical path is switched, the episcopic field diaphragm does not operate in interlocking mode.
Change the setting to ON. Refer to "20.8 Prohibiting the automatic Interlock function is disabled.
adjustment of the episcopic field diaphragm in switching of optical paths" in the reference manual. Change the setting to ON.
When zoom is operated, the episcopic field diaphragm does not operate in interlocking mode.
Interlock function is disabled.
Switches on 90i do not operate.
DIH-M is installed.
Refer to "20.9 Prohibiting the automatic adjustment of the episcopic field diaphragm when switching zooms" in the reference manual. Manually operate all functions except for the DIH-M shutter.
Switching of microscopy methods Problem Microscopy methods do not change. (Motorized device does not operate in interlocking mode.)
Possible causes
Remedy Change the setting to ON.
Interlock function is disabled.
Refer to "24.3 Canceling the automatic adjustments of motorized attachments" in the reference manual.
Switches Problem
Possible causes
Remedy Cancel the setting.
Switches do not operate.
A setting has been made to disable switch operations.
Refer to "25 Switches" in the reference manual.
Buzzer Problem
Possible causes
Remedy Cancel the setting.
Buzzer does not operate.
Setting has been set to disable buzzer.
Refer to "26 Buzzer" in the reference manual.
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Chapter 5 Troubleshooting Troubleshooting 5.2 Electrical
Autofocus Codes displayed during the use of autofocus indicate the following. Code
01
Detail
Remedy
Sample lacking sufficient contrast for
Slight focal difference may take place in some conditions.
autofocusing.
Perform manual focusing if this happens.
21
Nosepiece address is not specified.
Set the nosepiece into a clickable position.
22
Information for mounted objectives is
Set the information for mounted objectives in the
not available.
addresses of the nosepiece.
The address of a nosepiece is set with
The autofocus function of this product cannot be used with
liquid immersion objective information.
a liquid immersion objective.
24
Z axis initialization initialization error.
Initialize the Z axis.
25
Z axis in escape operation.
Cancel the Z axis escape operation.
26
Camera is not connected.
Check the connection of the camera to the 90i.
31
No response from camera.
Check the camera settings and others.
32
Microscopy condition is too bright.
Use the autofocus function under following conditions set
23
Focusing stops at the original position for the autofocus.
on the DS-L1 or DS-U1 + ACT-2U (when the sample is nearly in focus). Scene mode:
BF
Exposure time:
Under AE setting, the exposure time should come within 1/120 to 1/350 (in 1.3 MP display mode) when the sample is nearly in focus. (Adjust with ND filters, etc.)
33
Microscopy condition is too dark. Focusing stops at the original position for the autofocus.
Use the autofocus function under following conditions set on the DS-L1 or DS-U1 + ACT-2U (when the sample is nearly in focus). Scene mode: Exposure time:
BF Under AE setting, the exposure time should come within 1/120 to 1/350 (in 1.3 MP display mode) when the sample is nearly in focus. (Adjust with ND filters, etc.)
If a status code greater than "40" is displayed, contact your nearest Nikon representative.
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5
6 6.1
Care and Maintenance
Lens Cleaning Keep the lens free of dust, fingerprints, etc. Dirt on the lenses or filters will affect image quality. If any of the lenses become dirty, clean them by the procedure given below. •
Brush away dust with a soft brush or wipe away gently with gauze.
•
If fingerprints or grease gets on a lens, moisten a piece of soft, clean cotton cloth, lens tissue, or gauze with absolute alcohol (ethyl or methyl alcohol) and wipe.
•
Use petroleum benzine only to remove immersion oil from the objective. For optimum results, we recommend following up petroleum benzine with absolute alcohol (ethyl or methyl alcohol). If petroleum benzine is unavailable, use methyl alcohol alone. When using just methyl alcohol, note that surfaces will need to be wiped repeatedly to ensure complete removal of immersion oil. Usually, three or four times should be sufficient to clean the lens.
•
Never use petroleum benzine to clean the entrance lens at the bottom of the eyepiece tube or prism surface of the eyepiece tube.
•
Absolute alcohol and petroleum benzine are highly flammable. Be careful when handling these materials, particularly around open flames or when turning the power switch on or off.
•
6.2
6.3
Follow the instructions provided by the manufacturer manufacturer when using absolute alcohol.
Cleaning the Product •
We recommend using a silicon cloth to clean the product.
•
For stubborn dirt, dampen a piece of gauze with dilute neutral detergent and wipe gently.
•
Use of organic solvents on plastic parts may result in discoloration.
Disinfecting the Product •
For routine disinfection of the product, we recommend using 70% medical alcohol.
•
If contact occurs between a sample and the product, determine whether the sample is hazardous. If the sample is hazardous, follow the standard procedures for your laboratory.
•
Use of organic solvents on plastic parts may result in discoloration.
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Chapter 6 Care and Maintenance 6.4 Storage
6.4
Storage •
Store the product in a d ry location where mold is unlikely to form.
•
Store the objectives and eyepieces in a dry box or similar container with a drying agent.
•
Place the vinyl cover over the product to protect it from dust.
•
Switch off the power supply unit (press the power switch button so that it projects out) and wait for the lamphouse to cool before covering the product with the vinyl cover.
6.5
Periodic Inspections (fee charged) To maintain the peak performance of the microscope, we recommend periodic inspections. Contact your nearest Nikon representative for more information. (Parts and service charges apply for this service.)
6
85
7
Technical Specifications
7.1
Specifications
Nikon Microscope ECLIPSE 90i Model
ECLIPSE 90i
Main unit Optical system Main unit Illumination system
CFI60 system (infinity-corrected (infinity-corrected CF optical system) T-shaped, double-winged double-winged Flyeye illumination, with preset feature 12V 100W long-life halogen lamp (Average life: 2,000 hours) (Specified lamp housing: D-LH precentered lamp housing) (Specified lamp: PHILIPS 7724I or OSRAM HLX64623) NCB11, ND8, ND32 incorporated (replaceable) (replaceable)
Focusing section
Motorized focusing (can be switched between manual and auto modes.) Stroke: 27 mm (default: 17 mm)
Tube section
Binocular tube, ergonomic binocular tube (binocular section 100%, binocular: DSC port = 50:50) DSC port mountable to ergonomic binocular tube Trinocular eyepiece tube FUW (binocular: straight = 100:0, 0:100) Trinocular eyepiece tube TUW (binocular: straight = 100:0, 20:80, 0:100)
Nosepiece section
Motorized sextuple DIC nosepiece, motorized septuple nosepiece
Stage section
Rectangular stage (rectangular stage R: main centering unit)
Condenser section
Abbe condenser, achromat condenser, darkfield condenser (dry, oil), achromatic aplanat condenser, LWD condenser, motorized universal condenser 1-100X condenser, DIC condenser (oil), universal condenser (dry)
Eyepiece section
10x, field number: 22, 25
90i power supply
Model: D-PS 90i Power Supply Input ratings: 100-230V AC, ±10%, 50/60Hz, 2.8A – 1.1A
AC adapter for C-BOX
Manufacturer: ILAN ELECTRONICS ELECTRONICS LTD. Model: F1650K Rated input voltage: 100V-240V AC, 1.2A max., 50-60Hz Rated input voltage: 12V DC, 3.5A max Other: UL-listed, GS-approved, GS-approved, CE-certified CE-certified
Power cord
• When used in 100-120V areas
UL-listed detachable power cord set (3 conductor grounding Type SVT, AWG 18, 3m long maximum, rated at 125V AC minimum) • When used in 230V areas
EU/EN-approved EU/EN-approved 3-conductor power cord set (3 conductor grounding Type H05VV-F, AWG 18, 3m long maximum, rated at 250V AC minimum)
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Chapter 7 Technical Specifications 7.1 Specifications
Operating conditions
Temperature: 5 to 35°C Humidity: 85% RH max. (no condensation) Altitude: 2000 m max. Degree of pollution: Degree 2 Installation: Category II Protection class: Class I (D-PS 90i Power Supply) Indoor use only
Transport/storage conditions
Temperature: -20 to 60 °C
External dimensions and weight (main unit)
External dimensions: 300 (W) x 338 (H) x 384 (D) mm
Humidity: 90% RH max. (no condensation)
Weight: Approx. 12 kg
Safety standards
•
UL-Listed Product (UL61010A-1)
When using with the following attachments: attachments:
•
Meets FCC Port 15B Class A requirements.
•
•
D-DH digital imaging head M
•
D-DH-E motorized digital imaging head E
•
D-PS 90i power supply
•
D-NF-E 90i motorized ND filter unit
•
This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules.
D-LH precentered lamphouse
D-ND6-E motorized sextuple DIC nosepiece
•
D-N7-E Motorized Septuple Nosepiece
•
D-CUD-E Motorized Universal Condenser
These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. communications. Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense. •
This class A digital apparatus complies with Canadian ICES-003. Cet appreil numérique de classe A est conforme à la norme NMB-003 du Canada.
•
Complies with Australian EMC (AS/NZS2064 Group1 Class B).
CE Marking •
Meets EU IVDD (In vitro diagnostic medical device Directive) requirements. (GM-approved: In vitro diagnostic medical device)
•
Meets EU Low Voltage Directive requirements. requirements.
•
Meets EU EMC Directive (EN61326) requirements.
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7
Chapter 7 Technical Specifications Specificatio ns 7.1 Specifications
D-DH Digital Imaging Head M for Nikon Microscopes Model
D-DH Digital Imaging Head M
Optical system
Infinity-corrected Infinity-corrected CF optical system Eyepiece field number: φ25mm Variable intermediate magnification: magnification: Binocular tube 1X Front port 1x Rear port 0.8 to 2x (zoom ratio: 2.5, manual, with intelligent feature)
Light path switching
Manual 3-way (eyepiece section: 100%, front port 100%, rear port 100%) (with intelligent intelligent feature)
Eyepiece tube top viewing
25°
angle Fluorescent turret
Manual sextuple turret (with noise terminator, intelligent intelligent feature)
Aperture stop
Manual (unit detachable)
Field stop
Manual (unit detachable)
ND filter
Manual, 3 filters (slider type filters ND4, ND8, ND16)
Shutter
Motorized (controlled with external hand switch)
Analyzer slot
Equipped (side surface)
Polarizer slot
Equipped (side surface)
Compatible lamp housing
Hg, Xe, centered halogen (incompatible with precentered precentered type)
External connection
USB C1 interlock DIH control cable COM (for maintenance purposes)
Operating conditions
Temperature: Temperature: 0 to 40°C Humidity: 85% RH max. (no condensation) Altitude: 2000 m max. Degree of pollution: Degree 2 Installation: Installation: Category II Indoor use only
Transport/storage
Temperature: -20 to 60°C
conditions Humidity: 90% RH max. (no condensation) External dimensions and
External dimensions: 172 (W) x 185 (H) x 521 (D) mm (excluding projections)
weight Weight: Approx. 8 kg
88
Chapter 7 Technical Specifications Specificatio ns 7.1 Specifications
D-DH-E Motorized Digital Imaging Head E for Nikon Microscopes Model
D-DH-E Motorized Digital Imaging Head E
Optical system
Infinity-corrected Infinity-corrected CF optical system Eyepiece field number: φ25mm Variable intermediate magnification: magnification: Binocular tube 1X Front port 1x Rear port 0.8 to 2x (zoom ratio: 2.5, motorized, with intelligent feature)
Light path switching
Motorized 3-way (eyepiece (eyepiece section: 100%, front port 100%, rear port 100%) (with intelligent feature)
Eyepiece tube top viewing
25°
angle Fluorescent turret
Motorized sextuple turret (with noise terminator, intelligent feature)
Aperture stop
Manual (unit detachable)
Field stop
Motorized (unit not detachable) detachable)
ND filter
Manual, 3 filters (slider type filters ND4, ND8, ND16)
Shutter
Motorized
Analyzer
Motorized insertion/withdrawal insertion/withdrawal system (unit not detachable)
Polarizer slot
Equipped (side surface)
Compatible lamp housing
Hg, Xe, centered halogen (incompatible with precentered precentered type)
External connection
USB
7
C1 interlock DIH control cable COM (for maintenance purposes) Operating conditions
Temperature: Temperature: 5 to 35°C Humidity: 85% RH max. (no condensation) Altitude: 2000 m max. Degree of pollution: Degree 2 Installation: Installation: Category II Indoor use only
Transport/storage
Temperature: -20 to 60°C
conditions Humidity: 90% RH max. (no condensation) External dimensions and
External dimensions: 172 (W) x 185 (H) x 521 (D) mm (excluding projections)
weight Weight: Approx. 8 kg
89
Chapter 7 Technical Specifications Specificatio ns 7.1 Specifications
D-CUD-E Motorized universal condenser dry Model
D-CUD-E Motorized Universal Condenser Dry
Turret
Motorized septuple turret
Aperture stop
Motorized (φ 2 to 30 mm)
Operating conditions
Temperature: Temperature: 0 to 40°C Humidity: 85% RH max. (no condensation) Altitude: 2000 m max. Degree of pollution: Degree 2 Installation: Installation: Category II Indoor use only
Transport/storage
Temperature: -20 to 60°C
conditions Humidity: 90% RH max. (no condensation) External dimensions and
External dimensions: 180 (W) x 78 (H) x 184 (D) mm (excluding projections)
weight Weight: Approx. 1.2 kg
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