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Copyright © 2008, New Age International (P) Ltd., Publishers Published by New Age International (P) Ltd., Publishers All rights reserved. No part of this ebook ma y be reproduced in any form, by photostat, microfilm, xerography, or any other m eans, or incorporated into any information retrieval system, electronic or mecha nical, without the written permission of the publisher. All inquiries should be emailed to
[email protected] ISBN (13) : 978-81-224-2627-4 PUBLISHING FOR ONE WORLD NEW AGE INTERNATIONAL (P) LIMITED, PUBLISHERS 4835/24, Ansari Road, Daryaganj, N ew Delhi - 110002 Visit us at www.newagepublishers.com
Dedicated to PROF. DR. F.V. MANVI Secretary KLE Society, BELGAUM KARNATAKA. “To My First Pharmacy teacher with Love”
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FOREWORD Competitive Examinations are the order of the day. All Colleges conducting profe ssional courses at PG level are admitting students based on common entrance exam ination, which is of objective type. In Pharmacy, M.Pharm admissions are based o n qualifying the GATE enterance examination conducted by Govt. of India. In this book, The author has done good work in preparing several objective questions wh ich help the students to face the subject in the examination with poise and conf idence. The book is well balanced and consists of multiple choice questions from all the important topics like carbohydrate metabolism and other important Bioch emical aspects. The typesetting and quality of printing is good. The author is a lso well experienced in taking up this type of work. I recommend this book to al l the students preparing for GATE examination and also for Medical and Pharmacy College libraries. PROF. B.G. SHIVANANDA Principal AL-AMEEN COLLEGE OF PHARMACY BANGALORE.
WATER AND ELECTROLYTE BALANCE 289 PREFA PREFACE I have brought out this book basically for students who plan to appear for Bioch emistry in the entrance examinations like JIPMER and other Medical, Pharmacy, Ph ysiotherapy, Nursing and other Paramedical PG Entrance Examinations. There is a dearth of good entrance manual of Biochemistry for the above said examinations. Hence, I have prepared an exhaustive Question bank of around 5000 MCQs with answ ers covering a wide spectrum of basic Biochemical topics of the subject. Some of the important topics which are given a good coverage include Carbohydrate metab olism, Protein metabolism, Lipid metabolism, Nucleic acids, Enzymes, Vitamins an d Mineral metabolism. The objective questions are prepared based on the backgrou nd taken from previous question papers of Professional medical and Paramedical c ompetitive entrance examinations. The book serves as a ready reckoner for Bioche mistry as far as objective pattern is concerned. I feel satisfied if the book se rves the purpose for which it is intended. I have tried to minimize typographica l errors but still some must have crept in. If they are brought to my notice, I will be rectifying them in the next edition. Constructive Criticism is always we lcome G. Vidya Sagar
ACKNOWLEDGEMENTS I wish to express my profound gratitude and benevolence to the following who wer e the inspiring force in making this book a reality • Prof. Dr. Kishor Pramod Bhus ari • Sadhvi Shilapiji Principal, Nagpur College of Pharmacy Chair person, Veeraya tan Vidyapeeth, Nagpur. Jakhaniya, Kutch, Gujarat • Prof. Dr. R. Rangari • Prof. Dr. R.K. Goyal Principal, J.N. Chaturvedi College of Pharmacy L.M. College of Pharm acy Nagpur Ahmedabad, Gujarat • Prof. Dr. Anant Naik Nagappa • Prof. Dr. A.K. Saluja Pharmacy group, Birla Institute of Technology & A.R. College of Pharmacy Scienc es Vallabh Vidyanagar Pilani, Rajasthan Gujarat • Prof. Dr. Srinivas Rao • Prof. J.V .L.N. Sheshagiri Rao Principal, VEL’s College of Pharmacy Dept. of Pharmaceutical Sciences Chennai Andhra University, Vishakhapatnam, A.P. Finally, I express my g ratitude to Mr. Saumya Gupta. MD, New Age International (P) Limited, New Delhi, for his encouragement and support. Dr. G.Vidya Sagar
VALU ALUABLE SOME VALUABLE COMMENTS This book is very useful for students appearing for GATE Exams. Recommended read ing. Prof. Dr. Subhas C. Marihal Principal, Goa College of Pharmacy, Goa. Bioche mistry made simple in the form of multiple choice questions. Strongly recommende d. Prof. Dr. Vijaykumar Ishwar Hukkeri Principal, KLE College of Pharmacy, Hubli Dr. Vidya Sagar can be applauded for his untiring efforts in bringing out such a good book. Recommended for students and Library Dr. G. Devala Rao Principal, S idhartha College of Pharmaceutical Sciences Vijaywada, A.P. This book will be ve ry useful companion for students appearing for PG Medical, Pharmacy, Nursing and Physiotherapy competitive exams. Prof. Dr. T.K. Ravi Principal, Sri Ramakrishna Institute of Pharmaceutical Science Coimbatore. MCQs are well framed, mostly fr om previous entrance examinations. Commendable work. Prof. Madhukar R. Tajne Dep tt. of Pharmaceutical Sciences, Nagpur University, Nagpur
CONTENTS Preface (x) TO INTRODUCTION TO BIOCHEMISTRY CARBOHYDRATES CARBOHYDRATE METABOLISM CARBOHYDRA TES AND CARBOHYDRATE METABOLISM PROTEINS PROTEIN METABOLISM PROTEINS & PROTEIN M ETABOLISM FA ACID METABOLISM FATS & FATTY ACID METABOLISM VITAMINS VITAMINS ENZY MES CHAPTER 5 CHAPTER 6 CHAPTER 7 CHAPTER 8 CHAPTER 9 CHAPTER 4 CHAPTER 3 CHAPTER 2 Chapter 1 1 5 27 75 113 141 183 209 237 281 METABOLISM MINERAL METABOLISM METABOLISM HORMONE METABOLISM NUCLEIC ACIDS ACIDS ELECTROLYTE WATER & ELECTROLYTE BALANCE CHAPTER 10
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CHAPTER 1 INTRODUCTION TO BIOCHEMISTRY 1. A drug which prevents uric acid synthesis by inhibiting the enzyme xanthine o xidase is (A) Aspirin (C) Colchicine (B) Allopurinol (D) Probenecid 8. Which are the cholesterol esters that enter cells through the receptor-mediated endocytos is of lipoproteins hydrolyzed? (A) (B) (C) (D) Endoplasmin reticulum Lysosomes P lasma membrane receptor Mitochondria 2. Which of the following is required for crystallization and storage of the hor mone insulin? (A) Mn ++ (C) Ca++ (B) Mg ++ (D) Zn++ 9. Which of the following phospholipids is localized to a greater extent in the outer leaflet of the membrane lipid bilayer? (A) (B) (C) (D) Choline phosphoglyc erides Ethanolamine phosphoglycerides Inositol phosphoglycerides Serine phosphog lycerides 3. Oxidation of which substance in the body yields the most calories (A) Glucose (C) Protein (B) Glycogen (D) Lipids 4. Milk is deficient in which vitamins? (A) Vitamin C (C) Vitamin B2 (A) Phospho rus (C) Iron (B) Vitamin A (D) Vitamin K 10. All the following processes occur rapidly in the membrane lipid bilayer exce pt (A) (B) (C) (D) Flexing of fatty acyl chains Lateral diffusion of phospholipi ds Transbilayer diffusion of phopholipids Rotation of phospholipids around their long axes 5. Milk is deficient of which mineral? (B) Sodium (D) Potassium 6. Synthesis of prostaglandinsis is inhibited by (A) Aspirin (C) Fluoride (B) Ar senic (D) Cyanide 11. Which of the following statement is correct about membrane cholesterol? (A) The hydroxyl group is located near the centre of the lipid layer (B) Most of the cholesterol is in the form of a cholesterol ester (C) The steroid nucleus form forms a rigid, planar structure 7. HDL is synthesized and secreted from (A) Pancreas (C) Kidney (B) Liver (D) Mu scle
2 (D) The hydrocarbon chain of cholesterol projects into the extracellular fluid 1 2. Which one is the heaviest particulate component of the cell? (A) Nucleus (C) Cytoplasm (B) Mitochondria (D) Golgi apparatus MCQs IN BIOCHEMISTRY (C) Dihydrolipoyl dehydrogenase (C) All of these 21. The most active site of pro tein synthesis is the (A) Nucleus (C) Mitochondrion (B) Ribosome (D) Cell sap 13. Which one is the largest particulate of the cytoplasm? (A) (B) (C) (D) Lysos omes Mitochondria Golgi apparatus Entoplasmic reticulum 22. The fatty acids can be transported into and out of mitochondria through (A) Active transport (B) Facilitated transfer (C) Non-facilitated transfer (D) None of these 23. Mitochondrial DNA is (A) (B) (C) (D) Circular double stranded Circu lar single stranded Linear double helix None of these 14. The degradative Processess are categorized under the heading of (A) Anabolis m (C) Metabolism (B) Catabolism (D) None of the above 15. The exchange of material takes place (A) (B) (C) (D) Only by diffusion Only by active transport Only by pinocytosis All of these 24. The absorption of intact protein from the gut in the foetal and newborn anim als takes place by (A) Pinocytosis (C) Simple diffusion (B) Passive diffusion (D ) Active transport 16. The average pH of Urine is (A) 7.0 (C) 8.0 (B) 6.0 (D) 0.0 25. The cellular organelles called “suicide bags” are (A) Lysosomes (C) Nucleolus (B ) Ribosomes (D) Golgi’s bodies 17. The pH of blood is 7.4 when the ratio between H2CO3 and NaHCO3 is (A) 1 : 10 (C) 1 : 25 (B) 1 : 20 (C) 1 : 30 26. From the biological viewpoint, solutions can be grouped into (A) (B) (C) (D) Isotonic solution Hypotonic solutions Hypertonic solution All of these 18. The phenomenon of osmosis is opposite to that of (A) Diffusion (C) Affusion (B) Effusion (D) Coagulation 19. The surface tension in intestinal lumen between fat droplets and aqueous med ium is decreased by (A) Bile Salts (C) Conc. H2SO4 (B) Bile acids (D) Acetic aci d 27. Bulk transport across cell membrane is accomplished by (A) Phagocytosis (C) Extrusion (B) Pinocytosis (D) All of these 20. Which of the following is located in the mitochondria? (A) Cytochrome oxidas e (B) Succinate dehydrogenase 28. The ability of the cell membrane to act as a selective barrier depends upon (A) (B) (C) (D) The lipid composition of the membrane The pores which allows sma ll molecules The special mediated transport systems All of these
INTRODUCTION TO BIOCHEMISTRY 29. Carrier protein can (A) (B) (C) (D) Transport only one substance Transport m ore than one substance Exchange one substance to another Perform all of these fu nctions 3 35. Enzymes catalyzing electron transport are present mainly in the (A) (B) (C) (D) Ribosomes Endoplasmic reticulum Lysosomes Inner mitochondrial membrane 30. A lipid bilayer is permeable to (A) Urea (C) Glucose 31. The Golgi complex ( A) (B) (C) (D) Synthesizes proteins Produces ATP Provides a pathway for transpor ting chemicals Forms glycoproteins (B) Fructose (D) Potassium 36. Mature erythrocytes do not contain (A) Glycolytic enzymes(B) HMP shunt enzym es (C) Pyridine nucleotide(D) ATP 37. In mammalian cells rRNA is produced mainly in the (A) (B) (C) (D) Endoplasmic reticulum Ribosome Nucleolus Nucleus 32. The following points about microfilaments are true except (A) (B) (C) (D) Th ey form cytoskeleton with microtubules They provide support and shape They form intracellular conducting channels They are involved in muscle cell contraction 38. Genetic information of nuclear DNA is transmitted to the site of protein syn thesis by (A) rRNA (C) tRNA (B) mRNA (D) Polysomes 33. The following substances are cell inclusions except (A) Melanin (C) Lipids ( B) Glycogen (D) Centrosome 39. The power house of the cell is (A) Nucleus (C) Mitochondria (B) Cell membran e (D) Lysosomes 34. Fatty acids can be transported into and out of cell membrane by (A) Active t ransport (C) Diffusion (B) Facilitated transport (D) Osmosis 40. The digestive enzymes of cellular compounds are confined to (A) Lysosomes (C ) Peroxisomes (B) Ribosomes (D) Polysomes ANSWERS 1. B 7. B 13. B 19. A 25. A 31. D 37. C 2. D 8. B 14. B 20. D 26. D 32. C 38. D 3. D 9. A 15. D 21. B 27. D 33. D 39. C 4. A 10. C 16. B 22. B 28. D 34. B 40. A 5. C 11. C 17. B 23. A 29. D 35. D 6. A 12. A 18. A 24. A 30. A 36. C
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CHAPTER 2 ARBOHYDRATES CARBOHYDRATES AND ARBOHYDRATE ETABOLISM CARBOHYDRATE METABOLISM 1. The general formula of monosaccharides is (A) CnH2nOn (C) CnH2O2n (B) C2nH2On (D) CnH 2nO2n 9. Two sugars which differ from one another only in configuration around a singl e carbon atom are termed (A) Epimers (C) Optical isomers (B) Anomers (D) Stereoi somers 2. The general formula of polysaccharides is (A) (C6H 10O5)n (C) (C6H 10O6)n 3. The aldose sugar is (A) Glycerose (C) Erythrulose 4. A triose sugar is (A) Glyce rose (C) Erythrose 5. A pentose sugar is (A) Dihydroxyacetone (B) Ribulose (C) E rythrose (D) Glucose 6. The pentose sugar present mainly in the heart muscle is (A) Lyxose (C) Arabinose (A) Polymers (C) Proteins (A) 2 (C) 8 (B) Ribose (D) Xy lose (B) Acids (D) Oils (B) 4 (D) 16 (B) Ribose (D) Fructose (B) Ribulose (D) Di hydoxyacetone (B) (C6 H12O5)n (D) (C6 H10O6)n 10. Isomers differing as a result of variations in configuration of the —OH and —H o n carbon atoms 2, 3 and 4 of glucose are known as (A) Epimers (C) Optical isomer s (B) Anomers (D) Steroisomers 11. The most important epimer of glucose is (A) Galactose (C) Arabinose (B) Fruc tose (D) Xylose 12. α-D-glucose eto- ldo p irs
nd β -D-glucose
re (A) Stereoisomers (C) Anomers (B) Epimers (D) K
13. α-D-glucose + 1120 → + 52.50 ← + 190 βD-glucose for glucose ove represents (A) Opt ic l isomerism (B) Mut rot tion (C) Epimeris tion (D) D nd L isomerism 14. Comp ounds h ving the s me structur l formul ut differing in sp ti l configur tion re known s (A) Stereoisomers (C) Optic l isomers (B) Anomers (D) Epimers 7. Polys cch rides
re
8. The num er of isomers of glucose is
6 15. In glucose the orient tion of the —H nd —OH groups round the c r on tom 5 cent to the termin l prim ry lcohol c r on determines (A) (B) (C) (D) D or L s eries Dextro or levorot tory α nd β nomers Epimers
dj
MCQs IN BIOCHEMISTRY (C) Glucose + g l ctose (D) Glucose + m nnose 25. The monos cch ride units re l inked y 1 → 4 glycosidic link ge in (A) M ltose (C) Cellulose (B) Sucrose (D) Cel lo iose 16. The c r ohydr te of the (B) Fucose (D) M ltose
lood group su st nces is (A) Sucrose (C) Ar
26. Which of the following is B) M ltose (D) Treh lose
non-reducing sug r? (A) Isom ltose (C) L ctose (
17. Erythromycin cont ins (A) (B) (C) (D) Dimethyl ug r Sterol nd sug r Glycerol nd sug r 27. Which of the following is reh lose (D) Ag r 18. A sug r
inose
mino sug r Trimethyl
mino s
reducing sug r? (A) Sucrose (C) Isom ltose (B) T
lcohol is (A) M nnitol (C) Xylulose (B) Treh lose (D) Ar
inose
28. A diss cch ride formed y 1,1-glycosidic link ge etween their monos cch rid e units is (A) L ctose (C) Treh lose 29. (B) M ltose (D) Sucrose 19. The m jor sug r of insect hemolymph is (A) Glycogen (C) Treh lose (B) Pectin (D) Sucrose A diss cch ride formed y 1,1-glycosidic link ge etween their monos cch ride un its is (A) L ctose (C) Treh lose (B) M ltose (D) Sucrose 20. The sug r found in DNA is (A) Xylose (C) Deoxyri ose (B) Ri ose (D) Ri ulose 30. Mut rot tion refers to ch nge in (A) pH (C) Conduct nce (B) Optic l rot tion (D) Chemic l properties 21. The sug r found in RNA is (A) Ri ose (C) Ri ulose (B) Deoxyri ose (D) Erythr ose 22. The sug r found in milk is (A) G l ctose (C) Fructose 23. Invert sug r is (A ) L ctose (B) Sucrose (C) Hydrolytic products of sucrose (D) Fructose 24. Sucros e consists of (A) Glucose + glucose (B) Glucose + fructose (B) Glucose (D) L cto se 31. A polys cchh ride which is often c lled ulin (B) St rch (D) Dextrin
nim l st rch is (A) Glycogen (C) In
32. The homopolys cch ride used for intr venous infusion (A) Ag r (C) Pectin (B) Inulin (D) St rch
s pl sm
su stitute is
33. The polys cch ride used in ssessing the glomerul r fittr tion r te (GFR) is (A) Glycogen (C) Inulin (B) Ag r (D) Hy luronic cid
CARBOHYDRATES AND CARBOHYDRATE METABOLISM 34. The constituent unit of inulin is (A) Glucose (C) M nnose (B) Fructose (D) G l ctose 7 43. A polymer of glucose synthesized y the ction of leuconostoc mesenteroids i n sucrose medium is (A) Dextr ns (C) Limit dextrin (B) Dextrin (D) Inulin 35. The polys cch ride found in the exoskeleton of inverte r tes is (A) Pectin ( C) Cellulose (B) Chitin (D) Chondroitin sulph te 44. Glucose on reduction with sodium m lg m forms (A) Dulcitol (C) M nnitol (A) Glycoside (C) Gluconic cid (B) Sor itol (D) M nnitol nd sor itol (B) Glucos c ch ric cid (D) Glucuronic cid 36. Which of the following is D) Chitin
heteroglyc n? (A) Dextrins (C) Inulin (B) Ag r (
45. Glucose on oxid tion does not give 37. The glycos minoglyc n which does not cont in uronic cid is (A) (B) (C) (D) Derm t n sulph te Chondroitin sulph te Ker t n sulph te Hep r n sulph te 46. Oxid tion of g l ctose with conc HNO3 yields (A) Mucic cid (C) S cch ric c id (A) Sucrose (C) M ltose 48. St rch is (A) Polys cch ride (C) Dis cch ride ( B) Monos cch ride (D) None of these (B) Glucuronic cid (D) Gluconic cid (B) L ctose (D) Glucose 47. A positive Benedict’s test is not given
y
38. The glycos minoglyc n which does not cont in uronic cid is (A) (B) (C) (D) Hy luronic cid Hep rin Chondroitin sulph te Derm t n sulph te 39. Ker t n sulph te is found in x (B) Liver (D) Corne
und nce in (A) He rt muscle (C) Adren l corte
49. A positive Seliw noff’s test is o t ined with (A) Glucose (C) L ctose (A) Gluc ose (C) Sucrose (B) Fructose (D) M ltose (B) Fructose (D) L ctose 40. Repe ting units of hy luronic cid re (A) N- cetyl glucos mine nd D-glucur onic cid (B) N- cetyl g l ctos mine nd D-glucuronic cid (C) N- cetyl glucos m ine nd g l ctose (D) N- cetyl g l ctos mine nd L- iduronic cid 41. The pprox im te num er of r nches in mylopectin is (A) 10 (C) 40 (B) 20 (D) 80 50. Os zones
re not formed with the
51. The most und nt c r ohydr te found in n ture is (A) St rch (C) Cellulose ( B) Glycogen (D) Chitin 52. Imp ired ren l function is indic ted when the mount of PSP excreted in the first 15 minutes is (A) 20% (C) 40% 53. (B) 35% (D) 45% 42. In mylopectin the interv ls of glucose units of e ch 30–40 (B) 24–30 (D) 40–50
r nch is (A) 10–20 (C)
An e rly fe ture of ren l dise se is (A) Imp irment of the c p city of the tu ul e to perform osmotic work
8 (B) Decre se in m xim l tu ul r excretory c p city (C) Decre se in filtr tion f ctor (D) Decre se in ren l pl sm flow 54. ADH test is sed on the me surement of (A) (B) (C) (D) Specific gr vity of urine Concentr tion of ure in urine Conc entr tion of ure in lood Volume of urine in ml/minute 62. MCQs IN BIOCHEMISTRY Fructose is present in hydrolys te of (A) Sucrose (B) Inulin (C) Both of the o ve (D) None of these 63. A c r ohydr te found in DNA is (A) Ri ose (C) Ri ulose 64. Ri ulose is these (A) Ketotetrose (C) Ketopentose (B) Aldotetrose (D) Aldo pentose (B) Deoxyri ose (D) All of these 55. The specific gr vity of urine norm lly r nges from (A) 0.900–0.999 (C) 1.000–1.0 01 (B) 1.003–1.030 (D) 1.101–1.120 65. A c r ohydr te, commonly known s dextrose is (A) Dextrin (C) D-Glucose (A) Glucose (C) L ctose (B) D-Fructose (D) Glycogen (B) G l ctose (D) M ltose 56. Specific gr vity of urine incre ses in (A) (B) (C) (D) Di onic glomerulonephritis Compulsive polydypsi Hyperc lcemi
etes mellitus Chr
66. A c r ohydr te found only in milk is 67. A c r ohydr te, known commonly (B) Sucrose (D) L ctose
s invert sug r, is (A) Fructose (C) Glucose
57. Fix tion of specific gr vity of urine to 1.010 is found in (A) (B) (C) (D) 5 8. Di etes insipidus Compulsive polydypsi Cystinosis Chronic glomerulonephriti s 68. A heteropolys cchr ide lulose (D) Dextrin
mong the following is (A) Inulin (C) Hep rin (B) Cel
Addis test is the me sure of (A) Imp irment of the c p city of the tu ule to per form osmotic work (B) Secretory function of liver (C) Excretory function of live r (D) Activity of p renchym l cells of liver 69. The predomin nt form of glucose in solution is (A) (B) (C) (D) Acyclic form Hydr ted cyclic form Glucofur nose Glucopyr nose 59. Num er of stereoisomers of glucose is (A) 4 (C) 16 (B) 8 (D) None of these 70. An L-isomer of monos cch ride formed in hum n ody is (A) L-fructose (C) L-X ylose 71. (A) Joints (C) A domen (B) L-Erythrose (D) L-Xylulose (B) Br in (D) Mo uth 60. M ltose c n e formed in (D) All of these Hy luronic
y hydrolysis of (A) St rch (C) Glycogen 61. (B) Dextr
cid is found in
α –D–Glucuronic cid is present in (A) Hy luronic cid (C) Hep rin (B) Chondroitin sulph te (D) All of these 72. The c r on tom which ecomes symmetric when the str ight ch in form of mon os cch ride ch nges into ring form is known s
CARBOHYDRATES AND CARBOHYDRATE METABOLISM (A) (B) (C) (D) Anomeric c r on tom Epimeric c r on one of these
tom Isomeric c r on
tom N
9 82. L ct te formed in muscles c n e utilised through (A) (B) (C) (D) R poport-L ue eling cycle Glucose- l nine cycle Cori’s cycle Citric cid cycle 73. The sm llest monos cch ride h ving fur nose ring structure is (A) Erythrose (C) Glucose (B) Ri ose (D) Fructose 83. Glucose-6-phosph t se is not present in (A) (B) (C) (D) Liver nd kidneys Ki dneys nd muscles Kidneys nd dipose tissue Muscles nd dipose tissue 74. Which of the following is n epimeric p ir? (A) (B) (C) (D) Glucose tose Glucose nd g l ctose G l ctose nd m nnose L ctose nd m ltose
nd fruc
84. Pyruv te c r oxyl se is regul ted y (A) Induction (B) Repression (C) Allost eric regul tion(D) All of these 85. Fructose-2, 6- iphosph te is formed y the ction of (A) (B) (C) (D) Phosphofructokin se-1 Phosphofructokin se-2 Fructose i phosph te isomer se Fructose-1, 6- iphosph t se 75. α-Glycosidic these
ond is present in (A) L ctose (C) Sucrose (B) M ltose (D) All of
76. Br nching occurs in glycogen pproxim tely fter every (A) (B) (C) (D) Five glucose units Ten glucose units Fifteen glucose units Twenty glucose units 86. The highest concentr tions of fructose re found in (A) Aqueous humor (C) Sy novi l fluid (B) Vitreous humor (D) Semin l fluid 77. N–Acetylglucos mnine is present in (A) Hy luronic ndroitin sulph te (D) All of these
cid (C) Hep rin 78. (B) Cho
87. Glucose upt ke y liver cells is (A) Energy-consuming (B) A s tur le proces s (C) Insulin-dependent (D) Insulin-independent 88. Ren l threshold for glucose is decre sed in (A) Di etes mellitus (B) Insulinom (C) Ren l glycosuri (D) Al iment ry glycosuri 89. Active upt ke of glucose is inhi ited y (A) Ou in (C) Digoxin (B) Phlorrizin (D) Allox n Iodine gives 79. Amylose is f these
red colour with (A) St rch (C) Glycogen (B) Dextrin (D) Inulin constituent of (A) St rch (C) Glycogen (B) Cellulose (D) None o
80. Synovi l fluid cont ins (A) (B) (C) (D) Hep rin Hy luronic sulph te Ker tin sulph te
cid Chondroitin
90. Glucose-6-phosph t se is sent or deficient in (A) (B) (C) (D) Von Gierke’s d ise se Pompe’s dise se Cori’s dise se McArdle’s dise se 81. Gluconeogenesis is decre sed rine (D) Insulin
y (A) Gluc gon (C) Glucocorticoids (B) Epineph
10 91. De r nching enzyme is sent in (A) (B) (C) (D) Cori’s dise se Andersen’s dise s e Von Gierke’s dise se Her’s dise se MCQs IN BIOCHEMISTRY 100. An mphi olic p thw y mong the following is (A) HMP shunt (C) Citirc cycle (B) Glycolysis (D) Gluconeogenesis
cid
101. Cori’s cycle tr nsfers (A) (B) (C) (D) Glucose from muscles to liver L ct te from muscles to liver L ct te from liver to muscles Pyruv te from liver to muscl es 92. McArdle’s dise se is due to the deficiency of (A) (B) (C) (D) Glucose-6-phosph t se Phosphofructokin se Liver phosphoryl se muscle phosphoryl se 102. Excessive int ke of eth nol incre ses the r tio: (A) NADH : NAD+ (C) FADH2 : FAD 103. (B) NAD+ : NADH (D) FAD : FADH2 93. T utomeris tion is (A) Shift of hydrogen (B) Shift of c r on (C) Shift of o th (D) None of these 94. In essenti l pentosuri , urine cont ins (A) D-Ri ose (C ) L-Xylulose (B) D-Xylulose (D) D-Xylose Eth nol decre ses gluconeogenesis y (A) Inhi iting glucose-6-phosph t se (B) In hi iting PEP c r oxykin se (C) Converting NAD+ into NADH nd decre sing the v i l ility of pyruv te (D) Converting NAD+ into NADH nd decre sing the v il ili ty of l ct te 95. Action of s liv ry myl se on st rch le ds to the form tion of (A) M ltose ( B) M ltotriose (C) Both of the ove (D) Neither of these 96. Congenit l g l cto s emi c n le d to (A) (B) (C) (D) 97. Ment l ret rd tion Prem ture c t r ct De th All of the ove 104. Glycogenin is (A) (B) (C) (D) Uncoupler of oxid tive phosphoryl tion Polyme r of glycogen molecules Protein primer for glycogen synthesis Intermedi te in gl ycogen re kdown 105. During st rv tion, ketone odies Liver (B) Br in (D) All of these
re used
s
fuel
y (A) Erythrocytes (C)
Uridine diphosph te glucose (UDPG) is (A) (B) (C) (D) Required for met olism of g l ctose Required for synthesis of glucuronic cid A su str te for glycogen sy nthet se All of the ove 106. Anim l f t is in gener l (A) Poor in s tur ted nd rich in polyuns tur ted f tty cids (B) Rich in s tur ted nd poor in polyuns tur ted f tty cids (C) Ri ch in s tur ted nd polyuns tur ted f tty cids (D) Poor in s tur ted nd polyun s tur ted f tty cids 107. In the diet of di etic p tient, the recommended c r ohydr te int ke should prefer ly e in the form of 98. C t lytic ctivity of s liv ry myl se requires the presence of (A) Chloride ions (C) Iodide ions (B) Bromide ions (D) All of these 99. The following is ctively sor ed in the intestine: (A) Fructose (C) G l ct ose (B) M nnose (D) None of these
CARBOHYDRATES AND CARBOHYDRATE METABOLISM (A) Monos cch rides (B) Diss cch rides (C) Polys cch rides (D) All of these 108. O esity incre ses the risk of (A) Hypertension (B) Di etes mellitus (C) C rdio v scul r dise se (D) All of these 109. Worldwide, the most common vit min defici ency is th t of (A) Ascor ic cid (C) Vit min A (B) Folic cid (D) Vit min D 116 . He vy proteinuri occurs in (A) (B) (C) (D) (A) (B) (C) (D) Acute glomerulonep hritis Acute pyelonephritis Nephrosclerosis Nephrotic syndrome H mopolys cch rid es Hetropolys cch rides Proteins Amino cids (B) 40–-60° C (D) 80°–100° C (B) Thyrotoxicos is (D) Cretinism 11 117. Mucopolys cch rides
re
118. Bence-Jones protein precipit tes Myxoedem
t (A) 20°–40° C (C) 60°–80° C (A) Endemic goitre (C
110. Consumption of iodised s lt is recommended for prevention of (A) Hypertensi on (C) Endemic goitre (B) Hyperthyroidism (D) None of these 119. Serum cholesterol is decre sed in 111. Restriction of s lt int ke is gener lly recommended in (A) Di etes mellitu s (B) Hypertension (C) Cirrhosis of liver (D) Peptic ulcer 112. Polyuri c n occ ur in (A) Di etes mellitus (B) Di rrhoe (C) Acute glomerulonephritis (D) High fever 113. Norm l specific gr vity of urine is (A) 1.000–1.010 (C) 1.025–1.034 (B) 1 .012–1.024 (D) 1.035–1.045 120. The heptose ketose sug r formed s result of chemic l re ction in HMP shu nt: (A) Sedoheptulose (C) Glucoheptose (A) (C6H 12O6)n (C) (C6H 12O5)n (A) 4 (C) 12 123. (A) Fructose (C) Ri ose (B) G l ctoheptose (D) M nnoheptose (B) (C6 H10 O5)n (D) (C6 H19O6)n (B) 8 (D) 16 (B) G l ctose (D) Deoxyri ose 121. The gener l formul
for polys cch ride is
122. The num er of isomers of glucose is The epimers of glucose is 114. Specific gr vity of urine is r ised in ll of the following except (A) Di etes mellitus (B) Di etes insipidus (C) Dehydr tion (D) Acute glomerulonephriti s 115. Specific gr vity of urine is decre sed in (A) Di etes mellitus (B) Acute glomerulonephritis (C) Di rrhoe (D) Chronic glomerulonephritis 124. The intermedi te in hexose monophosph te shunt is (A) D-Ri olose (C) D-xylo se (A) L ctose (C) Inulin (B) D-Aro inose (D) D-lyxose (B) M ltose (D) St rch 125. Honey cont ins the hydrolytic product of 126. On oiling Benedict’s solution is not reduced L ctose (D) Fructose
y (A) Sucrose (C) M ltose (B)
12 127. Glycosides proteins
re found in m ny (A) Vit mins (C) Miner ls (B) Drugs (D) Nucleo
MCQs IN BIOCHEMISTRY 138. The component of c rtil ge nd corne is (A) (B) (C) (D) Ker tosulph te Cho ndroitin sulph te C dmium sulph te Antimony sulph te 128. G l ctose on oxid tion with conc. HNO3 produces (A) Gluconic cid (B) S cch ric cid (C) S cch ro L ctone (D) Mucic cid 129. The distinguishing test etwe en monos cch rides nd diss cch rides is (A) Bi l’s test (C) B rfoed’s test (B) Selw noff’s test (D) Hydrolysis test 139. Benedict’s test is less likely to give we kly positive results with concentr ted urine due to the ction of (A) Ure (C) Ammonium s lts (B) Uric cid (D) Pho sph tes 140. Active tr nsport of sug r is depressed lon te (B) Fum r te (D) Succin te
y the
gent: (A) Ox lo cet te (C) M
130. Cellulose is m de up of the molecules of (A) α-glucose (B) β-glucose (C) Both o f the ove (D) None of these 131. Iodine solution produces no color with (A) Ce llulose (C) Dextrin (B) St rch (D) Glycogen 141. The gener l test for detection of c r ohydr tes is (A) Iodine test (C) B rf oed test (A) Oedem (C) Rickets (B) Molisch test (D) Os zone test (B) Nephritis (D) Osteom litis 132. Glycogen structure includes (B) 8–14 (D) 12–18 142. Glucose
sorption m y
r nch in
etween–glucose units: (A) 6–12 (C) 6–10
e decre sed in
133. Amylose cont ins glucose units (A) 100–200 (C) 300–400 (B) 200–300 (D) 500–600 143. Glycogen synthet se ctivity is depressed Insulin (D) Fructokin se
y (A) Glucose (C) Cyclic AMP (B)
134. E ch r nch of mylopectin is t n interv l of glucose units: (A) 14–20 (C) 34–40 (A) Si lic cid (C) Glucuronic cid (B) 24–30 (D) 44–50 (B) Mucic cid (D) Hippu ric cid 144. The r nching enzyme cts on the glycogen when the glycogen ch in h s een lengthened to etween glucose units: (A) 1 nd 6 (C) 3 nd 9 (B) 2 nd 7 (D) 6 nd 11 135. N- cetylneur minic
cid is
n ex mple of
145. Cyclic AMP is formed from ATP y the enzyme denyl te cycl se which is cti v ted y the hormone: (A) Insulin (C) Testosterone (B) Epinephrine (D) Progester one 136. In pl ce of glucuronic cid chondroitin sulph te B cont ins (A) Gluconic id (C) Induronic cid (A) L ctose (C) Fructose (B) Gulonic cid (D) Sulphonic id (B) M ltose (D) Mucose
c c
146. Hexokin se h s high ffinity for glucose th n (A) Fructokin se (C) Glucok in se (B) G l ctokin se (D) All of the ove 137. Blood group su st nces consist of
147. Dihydroxy cetone phosph te y
nd glycer ldehyde-3-phosph te
re intercoverted
CARBOHYDRATES AND CARBOHYDRATE METABOLISM (A) (B) (C) (D) Triose isomer se Phosphotriose isomer se Diphosphotriose isomer se Dihydroxy cetone phosphoryl se 13 156. Which of the following met olite integr tes glucose ism? (A) Acetyl CoA (C) Citr te (B) Pyruv te (D) L ct te 148. Citr te is converted to isocitr te Zn ++ (B) Fe++ (D) Mg ++
y
nd f tty
cid met
ol
conit se which cont ins (A) C ++ (C)
157. Cere rosides consist of mostly of this sug r: (A) Glucose (C) G l ctose (B) Fructose (D) Ar inose 149. The re ction succinyl COA to succin te requires (A) CDP (C) GDP (A) Succin te (C) M l te (B) ADP (D) NADP+ (B) Fum r te (D) Ox lo cet te 158. Glucose will e converted into f tty cids if the diet h s excess of (A) C r ohydr tes (C) F t (B) Proteins (D) Vit mins 150. The c rrier of the citric
cid cycle is
159. The purple ring of Molisch re ction is due to (A) (B) (C) (D) Furfur l Furf ur l + α N pthol °C N pthol Furfurol + H2SO4 + α -N phthol 151. UDPG is oxidized to UDP glucuronic (A) FAD+ (C) NADP+ (B) NAD+ (D) ADP+
cid
y UDP dehydrogen se in presence of
152. G l ctose is phosphoryl ted y g l ctokin se to form (A) (B) (C) (D) G l ct ose-6-phosph te G l ctose-1, 6 diphosph te G l ctose-1-phosph te All of these 160. One of the following enzymes does not ch nge glycogen synth se to . (A) (B) (C) (D) Glycogen synth se kin ses 3, 4, 5 C 2+ c lmodulin phosphoryl se kin se C 2+ c lmodulin dependent protein kin se Glycogen phosphoryl se 153. The conversion of l nine to glucose is termed (A) (B) (C) (D) Glycolysis O xid tive dec r oxyl tion Specific dyn mic ction Gluconeogenesis 161. In EM p thw y -2 -phosphoglycer te is converted to (A) (B) (C) (D) Phospho enol pyruv te Enol pyruv te Di hydroxy cetone phosph te (DHAP) 1,3 isphosphogl ycer te 154. The lood sug r r to (A) Gluconeogenesis inhi ition of glomerul is one mole of glucose rty
ising ction of the hormones of supr ren l cortex is due (B) Glycogenolysis (C) Gluc gon-like ctivity (D) Due to r filtr tion 155. Under n ero ic conditions the glycolys yields __ moles of ATP. (A) One (C) Eight (B) Two (D) Thi
162. An neplerotic re ction which sust ins the v il ility of ox lo cet te is the c r oxyl tion of (A) Glut m te (C) Citr te (A) Seliw noff’s test (C) Molisch t est (B) Pyruv te (D) Succin te (B) Os zone test (D) None of these 163. Specific test for ketohexoses: 164. Two import nt yproducts of HMP shunt ADPH nd pentose sug rs
re (A) NADH
nd pentose sug rs (B) N
14 (C) Pentose sug rs nd 4 mem ered sug rs (D) Pentose sug rs nd sedoheptulose 16 5. Pyruv te dehydrogen se complex nd -ketoglut r te dehydrogen se complex requir e the following for their oxid tive dec r oxyl tion: (A) (B) (C) (D) COASH nd L ipoic cid NAD+ nd FAD COASH nd TPP COASH, TPP,NAD+,FAD, Lipo te MCQs IN BIOCHEMISTRY 172. Conversion of glucose to glucose-6phosph te in hum n liver is y (A) (B) (C ) (D) Hexokin se only Glucokin se only Hexokin se nd glucokin se Glucose-6-phos ph te dehydrogen se 173. The following is n enzyme required for glycolysis: (A) (B) (C) (D) Pyruv t e kin se Pyruv te c r oxyl se Glucose-6-phosph tose Glycerokin se 166. The four mem ered ldose sug r phosph te formed in HMP shunt p thw y is (A) Xylulose P (C) Erythrose P (B) Erythrulose P (D) Ri ulose P 174. The norm l glucose toler nce curve re ches pe k is (A) 15 min (C) 2 hrs (B) 1 hr (D) 2 ½ hrs 167. C ne sug r (Sucrose) injected into lood is (A) (B) (C) (D) ch nged to fruc tose ch nged to glucose undergoes no signific nt ch nge ch nged to glucose nd f ructose 175. Oxid tive dec r oxyl tion of pyruv te requires (A) (B) (C) (D) NADP+ Cytich romes pyridox l phosph te COASH 168. Pentose production is incre sed in (A) (B) (C) (D) HMP shunt Uromic thw y EM p thw y TCA cycle
cid p
176. Glucose toler nce is incre sed in (A) Di etes mellitus (B) Adren lectomy ( C) Acromeg ly (D) Thyrotoxicosis 177. Glucose toler nce is decre sed in (A) Di etes mellitus (B) Hypopituit risme (C) Addison’s dise se (D) Hypothyroidism 178. D uring glycolysis, Fructose 1, 6 diphosph te is decomposed y the enzyme: (A) Eno l se (B) Fructokin se (C) Aldol se (D) Diphosphofructophosph tose 179. The fol lowing enzyme is required for the hexose monophosph te shunt p thw y: (A) Glucos e-6-phosph t se (B) Phosphoryl se (C) Aldol se (D) Glucose-6-phosph te dehydroge n se 169. Conversion of Al nine to c r ohydr te is termed: (A) Glycogenesis (C) Glyco genolysis (B) Gluconeogenesis (D) Photosynthesis 170. The following is n enzyme required for glycolysis: (A) (B) (C) (D) Pyruv t e kin se Pyruv te c r oxyl se Glucose-6-phosph t se Glycerokin se 171. Our ody c n get pentoses from (A) (B) (C) (D) Glycolytic p thw y Uromic id p thw y TCA cycle HMP shunt
c
CARBOHYDRATES AND CARBOHYDRATE METABOLISM 180 Dehydrogen se enzymes of the hexose monophosph te shunt c (C) FAD specific (B) NADP+ specific (D) FMN specific
re (A) NAD+ specifi
15 189. The oxid tion of l ctic cid to pyruvic cid requires the following vit min deriv tive s the hydrogen c rrier. (A) Lithium pyrophosph te (B) Coenyzme A (C ) NAD+ (D) FMN 190. Physiologic l glycosuri is met with in (A) Ren l glycosuri (B) Aliment ry glycosuri (C) Di etes Mellitus (D) Allox n di etes 191. Two e x mples of su str te level phosphoryl tion in EM p thw y of glucose met olism re in the re ctions of (A) 1,3 isphosphoglycer te nd phosphoenol pyruv te (B) Glucose-6 phosph te nd Fructo-6-phosph te (C) 3 phosphoglycer ldehyde nd phosp hoenolpyruv te (D) 1,3 diphosphoglycer te nd 2-phosphoglycer te 192. The num er of molecules of ATP produced y the tot l oxid tion of cetyl CoA in TCA cycle is (A) 6 (C) 10 (B) 8 (D) 12 181. Under n ero ic conditions the glycolysis of one mole of glucose yields ___ ___moles of ATP. (A) One (C) Eight (B) Two (D) Thirty 182. Glycogen is converted to glucose-1phosph te y (A) UDPG tr nsfer se (B) Br nching enzyme (C) Phosphoryl se (D) Phosph t se 183. Which of the following is n ot n enzyme involved in glycolysis? (A) Euol se (C) Hexokin se (B) Aldolose (D) Glucose oxid se 184. Tric r oxylic cid cycle to e continuous requires the regener tion of (A) Pyruvic cid (B) ox lo cetic cid (C) α-oxoglut ric cid (D) M lic cid 185. Dehyd rogen tion of succinic cid to fum ric cid requires the following hydrogen c rr ier: (A) NAD+ (C) fl voprotein (B) NADP + (D) Glut thione 186. The tissues with the highest tot l glycogen content re (A) Muscle nd kidn eys (B) Kidneys nd liver (C) Liver nd muscle (D) Br in nd Liver 187. Rother test is not given y (A) β-hydroxy utyr te (B) ile s lts (C) Glucose (D) None of these 188. Gluconeogenesis is incre sed in the following condition: (A) Di ete s insipidus (B) Di etes Mellitus (C) Hypothyroidism (D) Liver dise ses 193. Su str te level phosphoryl tion in TCA cycle is in step: (A) Isocitr te deh ydrogen se (B) M l te dehydrogen se (C) Aconit se (D) Succin te thiokin se 194. F tty cids c nnot e converted into c r ohydr tes in the ody s the following re ction is not possi le. (A) Conversion of glucose-6-phosph te into glucose (B) Fructose 1, 6- isphosph te to fructose-6phosph te (C) Tr nsform tion of cetyl CoA to pyruv te (D) Form tion of cetyl CoA from f tty cids
16 195. Tissues form l ctic cid from glucose. This phenomenon is termed s (A) (B) (C) (D) Aero ic glycolysis Oxid tion Oxid tive phosphoryl tion An ero ic glycol ysis MCQs IN BIOCHEMISTRY 202. Amylo 1, 6 glucosid se is c lled (A) (B) (C) (D) Br nching enzyme de r nchi ng enzyme Gluc ntr nsfer se Phosphoryl se 196. One molecule of glucose gives ______ molecules of CO2 in EM-TCA cycle. (A) 6 (C) 1 (B) 3 (D) 2 203. Glucose enters the cells y (A) (B) (C) (D) 204. insulin independent tr nsp ort insulin dependent tr nsport enzyme medi ted tr nsport Both (A) nd (B) 197. One molecule of glucose gives ______ molecules of CO2 in one round of HMP s hunt. (A) 6 (C) 2 (B) 1 (D) 3 enzymes of Glycogen while eing cted upon y ctive phosphoryl se is converted first to (A ) Glucose (B) Glucose 1-phosph te nd Glycogen with 1 c r on less (C) Glucose-6phosph te nd Glycogen with 1 c r on less (D) 6-Phosphogluconic cid 198. The 4 r te limiting gluconeogenesis
re
(A) Glucokin se, Pyruv te c r oxyl e phosphoenol pyruv te c r oxykin se nd gluc ose-6-phosph t se (B) Pyruv te c r oxyl se, phosphoenol pyruv te c r oxykin se, fructose1,6 diphosph t se nd glucose-6-phosph t se (C) Pyruv te kin se, pyruv t e c r oxyl se, phosphoenol pyruv te c r oxykin se nd glucose-6-phosph t se (D) Phospho fructokin se, pyruv te c r oxyl se, phosphoenol pyruv te c r oxykin se nd fructose 1, 6 diphosph t se 199. For glycogenesis, Glucose should e converte d to (A) Glucuronic cid (C) UDP glucose (B) Pyruvic cid (D) Sor itol 205. When O2 supply is in dequ te, pyruv te is converted to (A) Phosphopyruv te (B) Acetyl CoA (C) L ct te (D) Al nine 206. Re ctiv tion of in ctive liver phosp horyl se is norm lly f voured y (A) Insulin (C) ACTH (B) Epinephrine (D) Gluc g on 207. Before pyruvic cid enters the TCA cycle it must CoA (C) α-ketoglut r te (B) L ct te (D) Citr te
e converted to (A) Acetyl
200. Fluoride inhi its ______ nd rrests glycolysis. (A) (B) (C) (D) (A) (B) (C ) (D) Glycer ldehyde-3-phosph te dehydrogen se Aconit se Enolose Succin te dehyd rogen se Glycogen synth se ‘ ’ is the phosphoryl ted cAMP converts glycogen synth se to ‘ ’ Insulin converts glycogen synth se to UDP glucose molecules inter ct nd grow into Glycogen tree 208. The hydrolysis of Glucose-6-phosph te is c t lysed y e which is found only in (A) (B) (C) (D) Liver, intestines leen nd dren ls Stri ted muscle Pl sm
specific phosph t s nd kidneys Br in, sp
201. One of the following st tement is correct: 209. The form tion of citr te from ox lo cet te (C) Condens tion (B) Reduction (D) Hydrolysis
nd
cetyl CoA is (A) Oxid tion
CARBOHYDRATES AND CARBOHYDRATE METABOLISM 210. Which one of the following is r te limiting enzyme of gluconeogenesis? (A ) (B) (C) (D) Hexokin se Phsophofructokin se Pyruv te c r oxyl se Pyruv te kin s e 17 218. Acetyl CoA is not used for the synthesis of (A) F tty (B) Cholesterol (D) Citric cid 219. The tot l glycogen content of the (B) 200 (D) 500
ody is
cid (C) Pyruvic
cid
out ______ gms. (A) 100 (C) 300
211. The num er of ATP produced in the succin te dehydrogen se step is (A) 1 (C) 3 (B) 2 (D) 4 220. The tot l Glucose in the 60–80
ody is ________ gms. (A) 10–15 (C) 40–50 (B) 20–30 (D)
212. Which of the following re ction gives l ctose? (A) (B) (C) (D) UDP g l ctos e nd glucose UDP glucose nd g l ctose Glucose nd G l ctose Glucose, G l ctose nd UTP 221. Pyruv te kin se requires ______ ions for m ximum (B) K + (D) Mg2+ 213. UDP Glucuronic cid is required for the ndroitin sulph tes Glycogen L ctose St rch
ctivity. (A) N + (C) C 2+
iosynthesis of (A) (B) (C) (D) Cho
222. ATP is ‘w sted’ in R poport-Lue erring cycle in RBCs s otherwise it will inhi it (A) (B) (C) (D) Phosphoglucomut se Phosphohexo isomer se Phosphofructo kin se Phosphoenol pyruv te c r oxy kin se 214. Which one of the following c n covert glucose to vit min C? (A) Al ino r ts (C) Monkeys (B) Hum ns (D) Guine pigs 223. The following co-enzyme is needed for the oxid tive dec r oxyl tion of keto cids: (A) NADP+ (B) TPP (C) Fol te coenzyme (D) Biotin coenzyme 224. Synthesis of Glucose from mino cids is termed s (A) Glycolysis (C) Glycogenesis (B) Glu coneogenesis (D) Lipogenesis 215. Which one of the following c nnot convert glucose to Vit min C? (A) Al ino r ts (C) Monkeys (B) Dogs (D) Cows 216. Tr nsketol se h s the coenzyme: (A) NAD (C) TPP + (B) FP (D) Pyridoxol phosph te 225. The following ex mples re import nt heteropolys cch rides except (A) Amylo pectin (C) Peptidoglyc n (B) Hep rin (D) Hy luronic cid 217. Two conditions in which gluconeogenesis is incre sed re (A) (B) (C) (D) Di etes mellitus nd therosclerosis Fed condition nd thyrotoxicosis Di etes me llitus nd St rv tion Alcohol int ke nd cig rette smoking 226. Whcih of the following fe tures re common to monos cch rides? (A) (B) (C) (D) Cont in symmetric centres Are of 2 types – ldoses nd ketoses Tend to exist s ring structures in solution Include glucose, g l ctose nd r ffinose
18 227. Polys cch rides (A) Cont in m ny monos cch ride units which m y or m y not e of the s me kind (B) Function m inly stor ge or structur l compounds (C) Ar e present in l rge mounts in connective tissue (D) All of these 228. The sorp tion of glucose in the digestive tr ct (A) Occurs in the sm ll intestine (B) Is stimul ted y the hormone Gluc gon (C) Occurs more r pidly th n the sorption o f ny other sug r (D) Is imp ired in c ses of di etes mellitus 229. UDP-Glucose is converted to UDPGlucuronic cid y (A) ATP (C) NADP+ (B) GTP (D) NAD+ MCQs IN BIOCHEMISTRY 234. Which of the following compound is positive llosteric modifier of the en zyme pyruv te c r oxyl se? (A) Biotin (C) Ox lo cet te (B) Acetyl CoA (D) ATP 235. A specific inhi itor for succin te dehydrogen se is (A) Arsinite (C) Citr t e (B) Melou te (D) Cy nide 236. Most of the met olic p thw ys re either n olic or c t olic. Which of t he following p thw ys is considered s “ mphi olic” in n ture? (A) Glycogenesis (C) Lipolysis (B) Glycolytic p thw y (D) TCA cycle 237. Tr nsketol se ctivity is ffected in (A) (B) (C) (D) Biotin deficiency Pyr idoxine deficiency PABA deficiency Thi mine deficiency 230. The enzymes involved in Phosphoryl tion of glucose to glucose 6- phosph te re (A) (B) (C) (D) Hexokin se Glucokin se Phosphofructokin se Both (A) nd (B) 238. The following met olic norm lities occur in Di etes mellitus except (A) (B) (C) (D) Incre sed pl sm FFA Incre sed pyruv te c r oxyl se ctiv te Decre sed lipogenesis Decre sed gluconeogenesis 231. In conversion of L ctic cid to Glucose, three re ctions of Glycolytic p th w y re circumvented, which of the following enzymes do not p rticip te? (A) (B) (C) (D) Pyruv te C r oxyl se Phosphoenol pyruv te c r oxy kin se Pyruv te kin s e Glucose-6-phosph t se 239. A su st nce th t is not n intermedi te in the form tion of D-glucuronic c id from glucose is (A) (B) (C) (D) Glucoss-1-p 6-Phosphoglucon te Glucose-6-p UD P-Glucose 232. The norm l resting st te of hum ns, most of the lood glucose s consumed y (A) Liver (C) Kidneys (B) Br in (D) Adipose tissue
urnt
s “fuel” i
240. The hydrolysis of Glucose-6-P is c t lysed y phosph t se th t is not for med in which of the following? (A) Liver (C) Muscle (B) Kidney (D) Sm ll intesti ne 233. A regul tor of the enzyme Glycogen synth se is (A) (B) (C) (D) Citric 2, 3 isphosphoglycer te Pyruv te GTP 241. An essenti l for converting Glucose to Glycogen in Liver is (A) L ctic (C) CTP (B) GTP (D) UTP
cid
cid
CARBOHYDRATES AND CARBOHYDRATE METABOLISM 242. Which of the following is su str te for ldol se ctivity in Glycolytic p thw y? (A) (B) (C) (D) Glycer ldehyde-3-p Glucose-6-p Fructose-6-p Fructose1, 6 - isphosph te 19 249. Which of the following st tements reg rding T.C.A cycle is true? (A) It is n n ero ic process (B) It occurs in cytosol (C) It cont ins no intermedi tes f or Gluconeogenesis (D) It is mphi olic in n ture 250. An llosteric enzyme resp onsi le for controlling the r te of T.C.A cycle is (A) (B) (C) (D) M l te dehydr ogen se Isocitr te dehydrogen se Fum r se Aconit se 243. The r tio th t pproxim tes the num er of net molecule of ATP formed per mo le of Glucose oxidized in presence of O2 to the net num er formed in scence of O2 is (A) 4 : 1 (C) 12 : 1 (B) 10 : 2 (D) 18 : 1 244. The “Prim quin sensitivity types of h emolytic n emi h s een found to rel te to reduced R.B.C ctivity of which enzyme? (A) (B) (C) (D) Pyruv te kin se de ficiency Glucose-6-phosph t se deficiency Glucose-6-p dehydrogen se deficiency H exokin se deficiency 251. The glycolysis is regul ted hofructokin se (D) All of these
y (A) Hexokin se (C) Pyruv te kin se (B) Phosp
252. How m ny ATP molecules will e required for conversion of 2-molecules of L ctic cid to Glucose? (A) 2 (C) 8 (B) 4 (D) 6 245. Which of the following hormones is not involved in c r ohydr te met (A) Cortisol (C) Glucogen (B) ACTH (D) V sopressin
olism?
253. Which of the following enzyme is not involved in HMP shunt? (A) (B) (C) (D) Glycer ldehyde-3-p dehydrogen se Glucose-6-p-dehydrogen se Tr nsketol se Phosph oglucon te dehydrogen se 246. Dehydrogen ses involved in HMP shunt NAD+ (D) FMN
re specific for (A) NADP+ (C) FAD (B)
247. Which of the following enzymes in Glycolytic p thw y is inhi ited y fluori de? (A) (B) (C) (D) Glycer ldehyde-3-p dehydrogen se Phosphoglycer te kin se Pyr uv te kin se Enol se 254. In presence of the following cof ctor, pyruv te c r oxyl se converts pyruv te to ox lo cet te: (A) (B) (C) (D) ATP, Protein nd CO2 CO2 nd ATP CO2 Protein 248. Out of 24 mols of ATP formed in TCA cycle, 2 molecules of ATP c n e formed t “su str te level” y which of the following re ction ? (A) (B) (C) (D) Citric c id→ Isocitric cid Isocitr te→ Ox lo cet te Succinic cid→ Fum r te Succinylc t→ Succini c cid 255. For conversion of ox lo cet te to phosphoenol pyruv te, high energy molecul e is required in the form of (A) GTP only (C) GTP (or) ITP (B) ITP only (D) None of these 256. If the more neg tive st nd rd reduction potenti l of ter the tendency to
redox p ir, the gre
20 (A) (B) (C) (D) To lose electrons To g in electrons To lose/g in electrons To lo se nd g in electrons MCQs IN BIOCHEMISTRY (C) The free energy ch nge, ∆G°, is equ l to the st nd rd free energy ch nge, ∆G° (D) Ke q is equ l to 1 264. An uncoupler of oxid tive phosphoryl tion such s dinitroph enol (A) Inhi its electron tr nsport nd ATP synthesis (B) Allow electron tr nsp ort to proceed without ATP synthesis (C) Inhi its electron tr nsport without imp irment of ATP synthesis (D) Speci lly inhi its cytochrome 265. All of the fol lowing st tements out the enzymic complex th t c rries out the synthesis of AT P during oxid tive phosphoryl tion re correct except (A) It is loc ted on the m trix side of the inner mitochondri l mem r ne (B) It is inhi ited y oligomycin (C) It c n exhi it ATP se ctivity (D) It c n ind molecul r O2 266. Glucokin s e (A) Is widely distri uted nd occurs in most m mm li n tissues (B) H s high km for glucose nd hence is import nt in the phosphoryl tion of glucose prim ril y fter ingestion of c r ohydr te rich me l (C) Is widely distri uted in Prok ryotes (D) None of these 267. The re ction c t lysed y phosphofructokin se (A) Is ctiv ted y high concentr tions of ATP nd citr te (B) Uses fruitose-1-phosp h te s su str te (C) Is the r te-limiting re ction of the glycolytic p thw y (D ) Is inhi ited y fructose 2, 6- isphosph te 268. Comp red to the resting st te, vigorously contr cting muscle shows (A) An incre sed conversion of pyruv te to l ct te (B) Decre sed oxid tion of pyruv te of CO2 nd w ter (C) A decre sed NAD H/NAD+ r tio (D) Decre sed concentr tion of AMP 257. Electron tr nsport nd phosphoryl tion c n e uncoupled y compounds th t i ncre se the perme ility of the inner mitochondri l mem r ne to (A) Electrons (C ) Uncouplers (B) Protons (D) All of these 258. The more positive the E0, the gre ter the tendency of the oxid nt mem er of th t p ir to (A) (B) (C) (D) Lose electrons G in electrons Lose (or) g in elect rons Lose nd g in electrons 259. The st nd rd free energy of hydrolysis of termin l phosph te group of ATP i s (A) –7,300 c l/mol (C) 10,000 c l/mol (B) –8,300 c l/mol (D) +7,300 c l/mol 260. The tr nsport of p ir of electrons from NADH to O2 vi the electron tr ns port ch in produces (A) –52,580 c l (C) 21,900 c l (B) –50,580 c l (D) +52,580 c l 261. Sufficient energy required to produce 3 ATP from 3 ADP 0 c l (C) 31,900 c l (B) 29,900 c l (D) 39,900 c l
nd 3 pi is (A) –21,90
262. The free energy ch nge, AG (A) Is directly proportion l to the st nd rd fre e energy ch nge, AG (B) Is equ l to zero t equili rium (C) C n only e c lcul t ed when the re ct nts nd products re present t 1mol/1 concentr tions (D) Is e qu l to –RT in keq 263. Under st nd rd conditions (A) The free energy ch nge ∆G°, is e qu l to 0 (B) The st nd rd free energy ch nge ∆G, is equ l to 0
CARBOHYDRATES AND CARBOHYDRATE METABOLISM 269. Which one of the following would e expected in pyruv te kin se deficiency? (A) (B) (C) (D) Incre sed levels of l ct te in the R.B.C Hemolytic nemi Decre sed r tio of ADP to ATP in R.B.C Incre sed phosphoryl tion of Glucose to Glucos e-6-phosph te 275. P steur effect is (A) (B) (C) (D) 21 Inhi ition of glycolysis Oxygen is involved Inhi ition of enzyme phosphofructoki n se All of these 270. Which one of the following st tements concerning glucose met olism is corr ect? (A) The conversion of Glucose to l ct te occurs only in the R.B.C (B) Gluco se enters most cells y mech nism in which N + nd glucose re co-tr nsported (C) Pyruv te kin se c t lyses n irreversi le re ction (D) An elev ted level of insulin le ds to decre sed level of fructose 2, 6- isphosph te in hep tocyte 2 71. Which one of the following compounds c nnot give rise to the net synthesis o f Glucose? (A) L ct te (C) α-ketoglut r te (B) Glycerol (D) Acetyl CoA 276. How m ny ATPs re produced in the conversion of phosphoenol pyruv te to cit r te? (A) 1 (C) 4 (B) 2 (D) 6 277. Reduced glut thione functions in R.B.Cs to (A) (B) (C) (D) Produce NADPH Re duce methemoglo in to hemoglo in Produce NADH Reduce oxidizing gents such s H2 O2 (B) Hist mine (D) Throxine 278. Phenyl l nine is the precursor of (A) L-DOPA (C) Tyrosine 279. D-M nnose is present in some pl nt products like (A) Resins (C) Mucil ge (B ) Pectins (D) Gums 272. Which of the following re ctions is unique to gluconeogenesis? (A) (B) (C) (D) L ct te Pyruv te Phosphoenol pyruv te pyruv te Ox lo cet te phosphoenol pyru v te Glucose-6-phosph te Fructose-6-phosph te 280. G l ctose is (D) Chitin
m in constituent of (A) Milk sug r (C) C ne sug r (B) Honey
273. The synthesis of glucose from pyruv te y gluconeogenesis (A) (B) (C) (D) R equires the p rticip tion of iotin Occurs exclusively in the cytosol Is inhi it ed y elev ted level of insulin Requires oxid tion/reduction of FAD 281. Glucos mine is n import nt constituent of (A) (B) (C) (D) Homopolys cch ri de Heteropolys cch ride Mucopolys cch ride Dextr n 274. The conversion of pyruv te to cetyl CoA nd CO2 (A) (B) (C) (D) Is reversi le Involves the p rticip tion of lipoic cid Depends on the coenzyme iotin Occ urs in the cytosol 282. Glycogen is present in in (D) Stom ch
ll
ody tissues except (A) Liver (C) Kidney (B) Br
283. Iodine test is positive for st rch, dextrin en (B) Ag r (D) Cellulose
nd (A) Mucoproteins (C) Glycog
22 284. The gener l formul for polys cch ride is (A) (C6H 10O5)n (C) (C6H 12O5)n ( A) Fructose (C) Ri ose (A) D-Ar inose (C) D-Xylose (B) (C6H 12C6)n (D) (C5 H10O 5)n (B) G l ctose (D) Deoxyri ose (B) D-Ri ose (D) L-Xylose MCQs IN BIOCHEMISTRY β−D glucose are related 294. α− α−D Glucose and β−
y (A) Epimers (C) Multirotation (B) An
(D) Ketoenol pair 285. Epimers of glucose is 295. The sta le ring formation in D Glucose involves (A) C 1 and C 4 (C) C 1 and C 5 (B) C 1 and C 2 (D) C 2 and C 5 286. Human heart muscle contains 287. The intermediate n hexose monophosphate shunt is (A) D Ri ulose (C) D xylos e (B) D Ara inose (D) D Lyxose 296. Reduction of Glucose with Ca++ in water produces (A) Sor itol (C) Mannitol (B) Dulcitol (D) Glucuronic acid 297. Starch and glycogen are polymers of (A) Fructose (C) α−D Glucose (B) Mannose (D ) Galactose 288. On oiling Benedict’s solution is not reduced Lactose (D) Fructose
y (A) Sucrose (C) Maltose (B)
298. Reducing a ility of car ohydrates is due to (A) Car oxyl group (B) Hydroxyl group (C) Enediol formation (D) Ring structure 299. Which of the following is n ot a polymer of glucose? (A) Amylose (C) Cellulose 300. Invert sugar is (A) (B) (C) (D) Lactose Mannose Fructose Hydrolytic product of sucrose (B) Inulin (D) De xtrin 289. The distinguishing test etween monosaccharides and dissaccharide is (A) Bi al’s test (C) Barfoed’s test (A) Glucose (C) Sucrose 291. Cori cycle is (A) (B) (C) (D) Synthesis of glucose reuse of glucose uptake of glycose Both (A) & (B) (B) S ucrose (D) Maltose (B) Seliwanoff’s test (D) Hydrolysis test (B) Mannose (D) Ri os e 290. Barfoed’s solution is not reduced
y
292. Cane sugar is known as (A) Galactose (C) Fructose 301 The car ohydrate reserved in human se (D) Inulin
ody is (A) Starch (C) Glycogen (B) Gluco
293. Which of the following is not reducing sugar? (A) Lactose (C) Sucrose (B) M altose (D) Fructose 302 A dissaccharide linked
α -1-4 Glycosi(B) Sucrose (D) M ltose
y deic linkages is (A) Lactose (C) Cellulose
CARBOHYDRATES AND CARBOHYDRATE METABOLISM 23 ANSWERS 1. A 7. A 13. B 19. C 25. A 31. D 37. C 43. A 49. B 55. B 61. C 67. B 73. B 79. A 85. B 91. A 97. D 103. C 109. B 115. B 121. B 127. B 133. C 139. B 145. B 151. B 157. C 163. A 169. B 175. D 181. B 187. A 193. D 199. C 205. C 211. B 217. C 223. B 229. B 235. B 241. D 247. D 2. A 8. D 14. A 20. C 26. D 32. A 38. B 44. B 50. C 56. A 62. C 68. C 74. B 80. B 86. D 92. D 98. A 104. C 110. C 116. B 122. D 128. D 134. B 140. C 146. C 152. C 158. A 164. B 170. A 176. B 182. C 188. B 194. C 200. C 206. D 212. A 218. C 224. B 230. D 236. D 242. D 248. D 3. A 9. A 15. A 21. A 27. C 33. C 39. D 45. A 51. C 57. D 63. B 69. D 75. B 81. D 87. D 93 . A 99. C 105. B 111. B 117. A 123. B 129. C 135. C 141. B 147. B 153. D 159. B 165. D 171. D 177. A 183. D 189. C 195. D 201. C 207. A 213. A 219. C 225. A 231 . C 237. B 243. B 249. D 4. A 10. A 16. B 22. D 28. C 34. B 40. A 46. A 52. A 58 . A 64. C 70. D 76. B 82. C 88. C 94. C 100. C 106. B 112. B 118. B 124. A 130. A 136. C 142. A 148. B 154. A 160. D 166. C 172. C 178. C 184. B 190. B 196. A 2 02. B 208. A 214. A 220. B 226. C 232. B 238. B 244. C 250. B 5. B 11. A 17. A 2 3. C 29. B 35. B 41. D 47. A 53. A 59. C 65. C 71. A 77. A 83. D 89. B 95. C 101 . B 107. C 113. B 119. B 125. C 131. A 137. C 143. C 149. B 155. B 161. A 167. C 173. A 179. D 185. C 191. A 197. B 203. D 209. C 215. C 221. B 227. D 233. C 23 9. B 245. D 251. D 6. A 12. C 18. A 24. B 30. B 36. B 42. B 48. A 54. A 60. D 66 . C 72. A 78. C 84. D 90. A 96. D 102. A 108. D 114. D 120. A 126. A 132. D 138. A 144. D 150. D 156. A 162. B 168. A 174. B 180. B 186. C 192. D 198. B 204. C 210. C 216. C 222.C 228. A 234. A 240. C 246. A 252. D
24 253. A 259. A 265. D 271. B 277. D 283. C 289. C 295. C 301. C 254. A 260. D 266 . B 272. C 278. C 284. A 290. C 296. A 302. D 255. C 261. A 267. C 273. A 279. D 285. B 291. D 297. C 256. A 262. B 268. A 274. B 280. A 286. C 292. B 298. A MCQs IN BIOCHEMISTRY 257. B 263. C 269. B 275. D 281. C 287. A 293. C 299. B 258. B 264. B 270. C 276 . C 282. B 288. A 294. B 300. D EXPLANATIONS FOR THE ANSWERS 7. A Polys cch rides re polymers of monos cch rides. They re of two types– hompo lys cch rides th t cont in single type of monos cch ride (e.g., st rch, insuli n, cellulose) nd heteropolys cch rides with two or more different types of mono s cch rides (e.g., hep rin, chondroitin sulf te). Mutorot tion refers to the ch nge in the specific optic l rot tion representing the interconversion of α- nd βnomers of D-glucose to n equili rium. St rch is polys cch ride composed of Dg lucose units held together y -glycosidic onds, (α 1→ 4 link ges; t r nching point s α 1→ 6 link ges). Hy luronic cid is the ground su st nce of synovi l fluid of joi nts. It serves s lu ric nts nd shock sor nt in joints. The process of shift ing hydrogen tom from one c r on to nother to produce enediols is referred t o s t utomeriz tion. Mucopolys cch rides (commonly known s glycos minoglyc ns) re heteropolys cch rides composed of sug r deriv tives (m inly mino sug rs n d uronic cids). The import nt mucopolys cch rides include hy luronic cid, hep rin, chondroitin sulf te, derm t n sulf te nd ker t n sulf te. Molisch test: It is gener l test for the detection of c r ohydr tes. The strong H2SO4 hydrolys es c r ohydr tes (poly- nd dis cch rides) to li er te monos cch rides. The mono s cch rides get dehydr ted to form furfur l (from pentoses) or hydroxy methylfur fur l (from hexoses) which condense with α-n phthol to form violet coloured comp lex. 163. A Seliw noff’s test: this is specific test for ketohexoses. Concentr t ed hydrochloric cid dehydr tes ketohexoses to form furfur l deriv tives which c ondense with resorcinol to give cherry red complex. 187. A Rother ’s test: Nitro prosside in lk line medium re cts with keto group of ketone odies ( cetone nd ceto cet te) to form purple ring. This test is not given y β-hydroxy utyr te. 203. D Two specific tr nsport systems re recognized for the entry of glucose i nto the cells. ( ) Insulin-independent tr nsport: This is c rrier medi ted upt ke of glucose which is not dependent on the hormone inslulin. This oper tes in hep tocytes, erythrocytes nd r in. ( ) Insulin-dependent tr nsport: This occur s in muscle nd dipose tissue. 230. D Hexokin se nd glucokin se re involved i n the phosphoryl tion of glucose to glucose 6phosph te. The enzyme hexokin se, p resent in lmost ll the tissues, c t lyses the phosphoryl tion of other hexose lso (fructose, m nnose). It h s low Km for su str tes ( out 0.1 mM) nd is inh i ited y glucose 6phosph te. In contr st, glucokin se is present in liver, c t lyses the phosphoryl tion of only glucose, h s high Km for glucose (10 mM) 30. B 48. A 71. A 93. A 117. A 141. B
CARBOHYDRATES AND CARBOHYDRATE METABOLISM nd is not inhi ited y glucose 6-phosph te. 251. D The three enzymes n mely hex okin se (or glucokin se), phosphofructokin se nd pyruv te kin se, c t lyzing th e irreversi le re ctions regul te glycolysis. Among these, phosphofructokin se i s the most regul tory. It is n llosteric enzyme inhi ited y ATP, citr te nd ctiv ted y AMP nd Pi. 275. D The inhi ition of glycolysis y oxygen is 25 referred to s P steur effect. This is due to inhi ition of the enzyme phosphofr uctokin se y ATP nd citr te (formed in the presence of O2 ) 291. D The cycle i nvolving the synthesis of glucose in liver from the skelet l muscle l ct te nd the reuse of glucose thus synthesized y the muscle for energy purposes is known s Cori Cycle.
This p ge intention lly left
l nk
CHAPTER 3 ROTEINS ROTEIN ETABOLISM PROTEINS AND PROTEIN METABOLISM 1. All proteins cont in the (A) (B) (C) (D) S me 20 mino cids Different mino cids 300 Amino cids occurring in n ture Only few mino cids Only L- α - mino cids Only D- mino cids DL-Amino cids Both (A) nd (B) (D) All mino cids cont in neg tively ch rged side ch ins 6. pH (isoelectric pH ) of l nine is (A) 6.02 (C) 6.8 (B) 6.6 (D) 7.2 2. Proteins cont in (A) (B) (C) (D) 7. Since the pK v lues for sp rtic cid re 2.0, 3.9 nd 10.0, it follows th t the isoelectric (pH) is (A) 3.0 (C) 5.9 (B) 3.9 (D) 6.0 3. The optic lly in ctive V line 8. Sulphur cont ining p r gine
mino
mino
cid is (A) Glycine (C) Threonine (B) Serine (D)
cid is (A) Methionine (C) V line (B) Leucine (D) As
4. At neutr l pH, mixture of mino cids in solution would e predomin ntly: ( A) (B) (C) (D) Dipol r ions Nonpol r molecules Positive nd monov lent Hydropho ic 9. An ex mple of sulphur cont ining mino cid is (A) (B) (C) (D) 2-Amino-3-merc ptoprop noic cid 2-Amino-3-methyl ut noic cid 2-Amino-3-hydroxyprop noic cid Amino cetic cid 5. The true st tement out solutions of mino cids t physiologic l pH is (A) All mino cids cont in oth positive nd neg tive ch rges (B) All mino cids c ont in positively ch rged side ch ins (C) Some mino cids cont in only positive ch rge 10. All the following re sulphur cont ining mino cids found in proteins excep t (A) Cysteine (C) Methionine (B) Cystine (D) Threonine 11. An
rom tic
mino
cid is (A) Lysine (C) T urine (B) Tyrosine (D) Arginine
28 12. The functions of pl sm D) oth (A ) nd (B)
l umin
re (A) Osmosis (C) Immunity (B) Tr nsport (
MCQs IN BIOCHEMISTRY 21. An mino cid th t does not form n α helix is (A) V line (C) Tyrosine (A) β-Al nine (C) Lysine (B) Proline (D) Tryptoph n 13. Amino cid with side ch in cont ining sic groups is (A) (B) (C) (D) 2-Amin o 5-gu nidov leric cid 2-Pyrrolidine c r oxylic cid 2-Amino 3-merc ptoprop noi c cid 2-Amino prop noic cid 22. An
mino
cid not found in proteins is (B) Proline (D) Histidine
23. In m mm li n tissues serine c n e iosynthetic precursor of (A) Methionin e (C) Tryptoph n (B) Glycine (D) Phenyl l nine 14. An ex mple of α - mino cid not present in proteins ut essenti l in m mm li n met olism is (A) (B) (C) (D) 3-Amino 3-hydroxyprop noic cid 2-Amino 3-hydroxy ut noic cid 2-Amino 4-merc pto ut noic cid 2-Amino 3-merc ptoprop noic cid 24. A v sodil ting compound is produced y the dec r oxyl tion of the : (A) Arginine (C) Glut mine (B) Asp rtic cid (D) Histidine 15. An essenti l (D) Serine
mino
mino
cid
cid in m n is (A) Asp rt te (C) Methionine (B) Tyrosine
25. Biuret re ction is specific for (A) –CONH-link ges (B) –CSNH2 group (C) –(NH)NH2 g roup (D) All of these 26. S k guchi’s re ction is specific for (A) Tyrosine (C) Ar ginine (B) Proline (D) Cysteine 16. Non essenti l mino cids (A) Are not components of tissue proteins (B) M y e synthesized in the ody from essenti l mino cids (C) H ve no role in the me t olism (D) M y e synthesized in the ody in dise sed st tes 17. Which one of the following is semiessenti l mino cid for hum ns? (A) V line (C) Lysine (B) Arginine (D) Tyrosine 27. Million-N sse’s re ction is specific for the enyl l nine (B) Tyrosine (D) Arginine
mino
cid: (A) Tryptoph n (C) Ph
28. Ninhydrin with evolution of CO2 forms lue complex with (A) Peptide C) Serotonin (B) α -Amino cids (D) Hist mine 18. An ex mple of pol r line
mino
ond (
cid is (A) Al nine (C) Arginine (B) Leucine (D) V
29. The most of the ultr violet sorption of proteins ove 240 nm is due to th eir content of (A) Tryptoph n (C) Glut m te (A) Anserine (C) Gluc gon (B) Asp rt te (D) Al nine (B) Glut thione (D) β -Lipoprotein 19. The mino cid with line (D) Threonine
nonpol r side ch in is (A) Serine (C) Asp r gine (B) V
30. Which of the following is 20. A ketogenic
mino
dipeptide?
cid is (A) V line (C) Leucine (B) Cysteine (D) Threonine
31. Which of the following is tocin (D) K llidin
tripeptide? (A) Anserine (C) Glut thione (B) Oxy
PROTEINS AND PROTEIN METABOLISM 32. A peptide which cts s potent smooth muscle hypotensive hione (C) Tryocidine (B) Br dykinin (D) Gr micidin-s
gent is (A) Glut t
29 43. The mino cid from which synthesis of the protein of h ir ker tin t kes pl ce is (A) Al nine (C) Proline (B) Methionine (D) Hydroxyproline 33. A tripeptide functioning s n import nt reducing gent in the tissues is (A ) Br dykinin (C) Tyrocidin (B) K llidin (D) Glut thione 44. In one molecule of 590 (D) 650
l umin the num er of
mino
cids is (A) 510 (C) 610 (B)
34. An ex mple of met lloprotein is (A) C sein (C) Gel tin (B) Cerulopl smin (D) S lmine 45. Pl sm proteins which cont in more th n 4% hexos mine (C) Mucoproteins (B) Glycoproteins (D) Orosomucoids
re (A) Microglo ulins
35. C r onic nhydr se is n ex mple of (A) Lipoprotein (C) Met lloprotein (B) P hosphoprotein (D) Chromoprotein 46. After rele sing O 2 t the tissues, hemoglo in tr nsports (A) (B) (C) (D) CO 2 nd protons to the lungs O2 to the lungs CO2 nd protons to the tissue Nutrien ts 36. An ex mple of chromoprotein is (A) Hemoglo in (C) Nuclein (B) Sturine (D) Gl i din 37. An ex mple of scleroprotein is (A) Zein (C) Glutenin (B) Ker tin (D) Ovoglo ulin 47. Ehlers-D nlos syndrome ch r cterized y hypermo ile joints nd skin norm l ities is due to (A) (B) (C) (D) A norm lity in gene for procoll gen Deficiency o f lysyl oxid se Deficiency of prolyl hydroxyl se Deficiency of lysyl hydroxyl se 38. C sein, the milk protein is (A) Nucleoprotein (C) Phosphoprotein (B) Chromop rotein (D) Glycoprotein 39. An ex mple of phosphoprotein present in egg yolk is (A) Ovo l umin (C) Ovovi tellin (B) Ovoglo ulin (D) Avidin 48. Proteins re solu le in (A) Anhydrous cetone(B) Aqueous lcohol (C) Anhydro us lcohol (D) Benzene 49. A cere l protein solu le in 70% lcohol ut insolu le in w ter or s lt solution is (A) Glutelin (C) Al umin (B) Prot mine (D) Gli din 40. A simple protein found in the nucleoproteins of the sperm is (A) Prol mine ( C) Glutelin 41. Histones re (A) (B) (C) (D) Identic l to prot mine Proteins ric h in lysine nd rginine Proteins with high molecul r weight Insolu le in w ter nd very dilute cids (B) Prot mine (D) Glo ulin 50. M ny glo ul r proteins re st le in solution inspite they l ck in (A) Disul phide onds (B) Hydrogen onds (C) S lt onds (D) Non pol r onds 51. The hydrog en onds etween peptide link ges of protein molecules re interfered y (A) G u nidine (C) Ox lic cid (B) Uric cid (D) S licylic cid 42. The protein present in h ir is (A) Ker tin (C) Myosin (B) El stin (D) Tropoc oll gen
30 52. Glo ul r proteins h ve completely folded, coiled polypeptide ch in nd the xi l r tio (r tio of length to re dth) is (A) Less th n 10 nd gener lly not gr e ter th n 3–4 (B) Gener lly 10 (C) Gre ter th n 10 nd gener lly 20 (D) Gre ter t h n 10 53. Fi rous proteins h ve xi l r tio (A) Less th n 10 (B) Less th n 10 nd gener lly not gre ter th n 3–4 (C) Gener lly 10 (D) Gre ter th n 10 54. E ch tu rn of α -helix cont ins the mino cid residues (num er): (A) 3.6 (C) 4.2 (B) 3.0 (D) 4.5 MCQs IN BIOCHEMISTRY 60. At the lowest energy level α -helix of polypeptide ch in is st ilised (A) By hydrogen onds formed etween the H of peptide N nd the c r onyl O of the resid ue (B) Disulphide onds (C) Non pol r onds (D) Ester onds 61. Both α-helix nd β-p le ted sheet conform tion of proteins were proposed y (A) (B) (C) (D) W tson n d Crick P uling nd Corey W ugh nd King Y.S.R o 62. The prim ry structure of fi roin, the princip l protein of silk worm fi res consists lmost entirely of (A) Glycine (C) Ker tin (B) Asp rt te (D) Tryptoph n 55. Dist nce tr veled per turn of α− α−helix in nm is (A) 0.53 (C) 0.44 (B) 0.54 (D) 0.4 8 63. Tertiary structure of a protein descri es (A) (B) (C) (D) The order of amino acids Location of disulphide onds Loop regions of proteins The ways of protein folding 56. Along the α-helix e ch
mino
cid residue
dv nces in nm
y (A) 0.15 (C) 0.12
(B) 0.10 (D) 0.20 64. In protein molecule the disulphide ond is not eduction Oxid tion Den tur tion X-r y diffr ction 57. The num er of helices present in ) 4
roken
y (A) (B) (C) (D) R
coll gen molecule is (A) 1 (C) 3 (B) 2 (D
58. In proteins the α-helix nd β-ple ted sheet re ex mples of (A) Prim ry structur e (B) Second ry structure (C) Terti ry structure (D) Qu tern ry structure 59. Th e -helix of proteins is (A) (B) (C) (D) A ple ted structure M de periodic y di sulphide ridges A non-periodic structure St ilised y hydrogen onds etween N H nd CO groups of the m in ch in 65. The technique for purific tion of proteins th t c n e m de specific for g iven protein is (A) (B) (C) (D) Gel filtr tion chromotogr phy Ion exch nge chrom togr phy Electrophoresis Affinity chrom togr phy 66. Den tur tion of proteins results in (A) (B) (C) (D) Disruption of prim ry st ructure Bre kdown of peptide onds Destruction of hydrogen onds Irreversi le ch nges in the molecule
PROTEINS AND PROTEIN METABOLISM 67. Cerulopl smin is (A) α1-glo ulin (C) β-glo ulin (B) α2-glo ulin (D) None of these 31 77. A lipoprotein inversely rel ted to the incidence of coron ry s is (A) VLDL (C) LDL (B) IDL (D) HDL 68. The lipoprotein with the f stest electrophoretic mo ility glyceride content is (A) Chylomicron (C) IDL (B) VLDL (D) HDL
rtherosclerosi
nd the lowest tri
78. The prim ry iochemic l lesion in homozygote with f mili l hypercholesterole mi (type II ) is (A) Loss of feed ck inhi ition of HMG reduct se (B) Loss of polipoprotein B (C) Incre sed production of LDL from VLDL (D) Function l defici ency of pl sm mem r ne receptors for LDL 79. In et lipoproteinemi , the ioch emic l defect is in (A) (B) (C) (D) Apo-B synthesis Lipprotein lip se ctivity C holesterol ester hydrol se LCAT ctivity 69. The lipoprotein DL (D) IDL
ssoci ted with
70. The polipoprotein which -IV (D) D
cts
ctiv tion of LCAT is (A) HDL (C) VLDL (B) L
s
ctiv tor of LCAT is (A) A-I (C) C-II (B) A
71. The polipoprotein which cts s Apo-A (C) Apo-C (B) Apo-B (D) Apo-D
cti tor of extr hep tic lipoprotein is (A)
72. The polipoprotein which forms the integr l component of chylomicron is (A) B-100 (C) C (B) B-48 (D) D 80. F mili l hypertri cylglycerolemi is ssoci ted with (A) (B) (C) (D) Over p roduction of VLDL Incre sed LDL concentr tion Incre sed HDL concentr tion Slow c le r nce of chylomicrons 73. The polipoprotein which from the integr l component of VLDL is (A) B-100 (C ) A (B) B-48 (D) D 81. For synthesis of prost gl ndins, the essenti l f tty cids give rise to f tty cid cont ining (A) 12 c r on toms (B) 16 c r on toms (C) 20 c r on toms (D) 24 c r on toms 82. All ctive prost gl ndins h ve t le st one dou le ond etween positions (A) 7 nd 8 (C) 13 nd 14 (B) 10 nd 11 (D) 16 nd 17 74. The polipoprotein which B) B-100 (D) C
cts
s lig nd for LDL receptor is (A) B-48 (C) A (
75. Serum LDL h s een found to e incre sed in (A) (B) (C) (D) O structive j un dice Hep tic j undice Hemolytic j undice M l sorption syndrome 83. Norm l r nge of pl sm tot l phospholipids is (A) 0.2–0.6 mmol/L (B) 0.9–2.0 mmo l/L (C) 1.8–5.8 mmol/L (D) 2.8–5.3 mmol/L 84. HDL2 h ve the density in the r nge of (A) 1.006–1.019 (C) 1.032–1.063 (B) 1.019–1.032 (D) 1.063–1.125 76. A lipoprotein ssoci ted with high incidence of coron ry (A) LDL (C) IDL (B) VLDL (D) HDL
therosclerosis is
32 85. β-lipoproteins h ve the density in the r nge of (A) 0.95–1.006 (C) 1.019–1.063 (B) 1.006–1.019 (D) 1.063–1.125 MCQs IN BIOCHEMISTRY 96 Pepsin cts on den tured proteins to produce (A) (B) (C) (D) Proteoses ptones Polypeptides Peptides Dipeptides
nd pe
86. IDL h ve the density in the r nge of (A) 0.95–1.006 (C) 1.019–1.032 (B) 1.006–1.01 9 (D) 1.032–1.163 97. Renin converts c sein to p r c sein in presence of (A) C ++ (C) N + (A) Tryp sin (C) El st se (B) Mg ++ (D) K + (B) Chymotrypsin (D) El st se 87. Aspirin inhi its the ctivity of the enzyme: (A) Lipoxygen se (B) Cyclooxyge n se (C) Phospholip e A1 (D) Phospholip se A2 88. A ’suicide enzyme’ is (A) Cycloxyg en se (B) Lipooxygen se (C) Phospholip se A1 (D) Phospholip se A2 89. In dipose tissue prost gl ndins decre se (A) Lipogenesis (B) Lipolysis (C) Gluconeogenesi s (D) Glycogenolysis 90 The optim l pH for the enzyme pepsin is (A) 1.0–2.0 (C) 5. 2– 6.0 (B) 4.0–5.0 (D) 5.8–6.2 98. An expopeptid se is 99. The enzyme trypsin is specific for peptide onds of (A) (B) (C) (D) B sic m ino cids Acidic mino cids Arom tic mino cids Next to sm ll mino cid resid ues 100. Chymotrypsin is specific for peptide onds cont ining (A) (B) (C) (D) Unch rged mino cid residues Acidic mino cids B sic mino cid Sm ll mino cid re sidues 91. Pepsinogen is converted to ++
ctive pepsin
y (A) HCl (C) C
(B) Bile s lts (D) Enterokin se 101. The end product of protein digestion in G.I.T. is (A) Dipeptide (C) Polypep tide (B) Tripeptide (D) Amino cid 92. The optim l pH for the enzyme rennin is (A) 2.0 (C) 8.0 (B) 4.0 (D) 6.0 93. The optim l pH for the enzyme trypsin is (A) 1.0–2.0 (C) 5.2–6.2 (B) 2.0–4.0 (D) 5 .8–6.2 102. N tur l L-isomers of mino cids re sor ed from intestine y (A) P ssive diffusion (B) Simple diffusion (C) F cili ted diffusion(D) Active process 103. A norm lities of lood clotting re (A) H emophili (C) Gout (B) Christm s dise se (D) Both (A) nd (B) 94. The optim l pH for the enzyme chymotrypsin is (A) 2.0 (C) 6.0 (B) 4.0 (D) 8. 0 95 Trypsinogen is converted to le s lts (D) Mg ++
ctive trypsin
y (A) Enterokin se (C) HCl (B) Bi
104. An import nt re ction for the synthesis of mino cid from c r ohydr te int ermedi tes is tr ns min tion which requires the cof ctor: (A) Thi min (C) Ni cin (B) Ri ofl vin (D) Pyridox l phosph te
PROTEINS AND PROTEIN METABOLISM 105. The m in sites for oxid tive de min tion re (A) Liver nd kidney (B) Skin nd p ncre s (C) Intestine nd m mm ry gl nd (D) Lung nd spleen 106. A positive nitrogen l nce occurs (A) In growing inf nt (B) Following surgery (C) In dv nced c ncer (D) In kw shiork r 107. The m in site of ure synthesis in m mm ls i s (A) Liver (C) Intestine (B) Skin (D) Kidney 33 113. Control of ure cycle involves the enzyme: (A) (B) (C) (D) C r moyl phosph te synthet se Ornithine tr nsc r moyl se Argininosuccin se Argin se 114. Tr nsfer of the c r moyl moiety of c r moyl phosph te to ornithine is c t lysed y liver mitochondri l enzyme: (A) (B) (C) (D) C r moyl phosph te synt het se Ornithine tr nsc r moyl se N- cetyl glut m te synthet se N- cetyl glut m te hydrol se 115. A compound serving link etween citric l te (C) Succin te (B) Citr te (D) Fum r te
cid cycle
nd ure
cycle is (A) M
108. The enzymes of ure synthesis re found in (A) Mitochondri only (B) Cytoso l only (C) Both mitochondri nd cytosol (D) Nucleus 109. The num er of ATP requ ired for ure synthesis is (A) 0 (C) 2 (B) 1 (D) 3 116. The 2 nitrogen toms in ure re contri uted y (A) (B) (C) (D) Ammoni glut m te Glut mine nd glut m te Ammoni nd sp rt te Ammoni nd l nine
nd
117. In c rcinoid syndrome the rgent ffin tissue of the domin l c vity overpr oduce (A) Serotonin (C) Trypt mine (B) Hist mine (D) Tyrosine 110. Most of the mmoni rele sed from L-αα mino cids reflects the coupled ction of tr ns min se nd (A) L-glut m te dehydrogen se (B) L- mino cid oxid se (C) H istid se (D) Serine dehydr t se 111. In ure synthesis, the mino cid functioni ng solely s n enzyme ctiv tor: (A) N- cetyl glut m te (B) Ornithine (C) Citru lline (D) Arginine 112. The enzyme c r moyl phosph te synthet se requires (A) M g ++ (C) N + 118. Tryptoph n could e considered s precursor of (A) Mel notonin (C) Mel nin (B) Thyroid hormones (D) Epinephrine 119. Conversion of tyrosine to dihydroxyphenyl l nine is c t lysed y tyrosine h ydroxyl se which requires (A) NAD (C) ATP (B) FAD (D) Tetr hydro iopterin 120. The r te limiting step in the iosynthesis of c techol mines is (A) (B) (C) (D) Dec r oxyl tion of dihydroxyphenyl l nine Hydroxyl tion of phenyl l nine Hy droxyl tion of tyrosine Oxid tion of dop mine (B) C ++ (D) K +
34 121. The enzyme dop mine β-oxid se which c t lyses conversion of dop mine to norep inephrine requires (A) Vit min A (C) Vit min E (B) Vit min C (D) Vit min B12 MCQs IN BIOCHEMISTRY 130 Chemic l score of protein zein is (A) 0 (C) 60 (B) 57 (D) 70 131. Biologic l v lue of egg white protein is (A) 94 (C) 85 (B) 83 (D) 77 122. In hum ns the sulphur of methionine nd cysteine is excreted m inly s (A) Ethere l sulph te (B) Inorg nic sulph te (C) Sulphites (D) Thioorg nic compound 123. Sm ll mount of urin ry ox l tes is contri uted y the mino cid: (A) Glyc ine (C) Al nine (B) Tyrosine (D) Serine 132. Net protein utilis tion of egg protein is (A) 75% (C) 91% (B) 80% (D) 72% 133. Net protein utiliz tion of milk protein is (A) 75% (C) 86% (B) 80% (D) 91% 134. A limiting mino cid is n essenti l mino cid (A) Th t is most deficient in proteins (B) Th t is most excess in proteins (C) Th t which incre ses the gr owth (D) Th t which incre ses the weight g in 135. The limiting mino cid of ri ce is (A) Lysine (C) Phenyl l nine (B) Tryptoph n (D) Tyrosine 124. The mino cid which detoxic ted enzoic cid to form hippuric Glycine (C) Serine (B) Al nine (D) Glut mic cid
cid is (A)
125. The mino cids involved in the synthesis of cre tin re (A) Arginine, glyc ine, ctive methionine (B) Arginine, l nine, glycine (C) Glycine, lysine, methi onine (D) Arginine, lysine, methionine 126. Chemic l score of egg proteins is co nsidered to e (A) 100 (C) 50 (B) 60 (D) 40 136. The limiting mino ysteine (D) Threonine 137. Pulses h n
cid of fish proteins is (A) Tryptoph n (C) Lysine (B) C
re deficient in (A) Lysine (C) Methionine (B) Threonine (D) Tryptop
127. Chemic l score of milk proteins is (A) 70 (C) 60 (B) 65 (D) 40 138. A tr ce element deficient in the milk is (A) M gnesium (C) Zinc (B) Copper (D) Chloride 128. Chemic l score of proteins of
eng l gr m is (A) 70 (C) 44 (B) 60 (D) 42
139. A conjug ted protein present in the egg yolk is (A) Vitellin (C) Al uminoid s (B) Livetin (D) Ovo-mucoid 129. Chemic l score of protein gel tin is (A) 0 (C) 57 (B) 44 (D) 60 140. The chief protein of cow’s milk is (A) Al umin (C) Livetin (B) Vitellin (D) C sein
PROTEINS AND PROTEIN METABOLISM 141. A w ter solu le vit min deficient in egg is (A) Thi min (C) Ascro ic cid 42. Pulses re rich in (A) Lysine (C) Tryptoph n 143. Milk is deficient in (A) it min B1 (C) Sodium 144. Milk is deficient in (A) C lcium (C) Sodium (B) Iron D) Pot ssium (B) Vit min B2 (D) Pot ssium (B) Methionine (D) Phenyl l nine (B) i of lvin (D) Co l min 35 153. In the tot l proteins, the percent ge of B) 30–45 (D) 80–90
l umin is
154. In the tot l proteins percent ge of glo ulin is (B) 1.2–2.0% (D) 5.0–10.0%
1 V ( R
out (A) 20–40 (C) 50–70 (
out (A) 0.2–1.2% (C) 2.4–4.4%
α1 155. In the tot l proteins the percent ge of γ 1–10.1% (B) 10.0–21.0% (D) 1.2–2.0%
lobulin is about (A) 2.4–4.4% (C) 6.
145. When net protein utilization (NPU) is low, the requirements for proteins ar e (A) Hi h (C) Low (A) 1.4% (C) 3.4% (A) 2.5% (C) 4.5% (A) 30% (C) 50% (A) 12% ( C) 10–11% (B) Moderate (D) Supplementary (B) 2.4% (D) 4.4% (B) 3.5% (D) 5.5% (B) 4 0% (D) 60% (B) 33% (D) Traces 156. Most frequently the normal albumin lobulin ratioratio (A : G) is (A) 1.0 : 0.8 (C) 2.0 : 1.0 (B) 1.5 : 1.0 (D) 2.4 : 1.0 146. Protein content of human milk is about 157. In Thymol turbidity test the protein involved is mainly (A) Albumin (C) α2-Gl o ulin (B) α1-Glo ulin (D) β Glo ulin 147. Protein content of cow’s milk is
out
158. In qu tern ry structure, su units re linked y (A) Peptide ent onds (B) Disulphide onds (D) Non-cov lent onds 148. Protein content of soy
e n is
onds (C) Cov l
out
159. Molecul r weight of hum n l umin is out (A) 156,000 (C) 69,000 (A) Anion (C) Zwitterion (B) 90,000 (D) 54,000 (B) C tion (D) None of these 149. Lipid content of egg white is 160. At isoelectric pH,
n
mino
cid exists
s
150. The recommended d ily llow nce (RDA) of proteins for n dult m n is (A) 7 0 gms (C) 40 gms (A) Lysine (C) Glycine (B) 50 gms (D) 30 gms (B) Bile cids (D) Al nine 161. A disulphide ond c n e formed etween (A) (B) (C) (D) (A) (B) (C) (D) Two methionine residues Two cysteine residues A methionine nd cysteine residue A ll of these Insolu le Biologic lly non-function l Unfolded All of the ove 151. The
sic
mino
cids
re
152. The d ily c loric requirement for the norm l (C) 2500 (B) 2100 (D) 2900 162 A co gul ted protein is
dult fem le is
out (A) 1500
36 163. At pH elow the isoelectric point, n mino cid exists s (A) C tion (B) Anion (C) Zwitterion (D) Undissoci ted molecule 164. An mino cid h ving hyd rophilic side ch in is (A) Al nine (C) Methionine (B) Proline (D) Serine (C) Ch peronins (D) All of these MCQs IN BIOCHEMISTRY 172. Prim ry structure of protein is formed y (A) Hydrogen onds (B) Peptide onds (C) Disulphide onds (D) All of these 173. α-Helix is formed y (A) Hydrogen onds (B) Hydropho ic onds (C) Electrost tic onds (D) Disulphide onds 174. G lutelins re present in (A) Milk (C) Me t (B) Eggs (D) Cere ls 165. An mino cid th t does not t ke p rt in α helix form tion is (A) Histidine ( C) Proline (B) Tyrosine (D) Tryptoph n 166. A protein rich in cysteine is (A) Coll gen (C) H emoglo in (B) Ker tin (D) Gel tin 175. Arom tic mino cids c n e detected y (A) S k guchi re ction (B) Millon-N sse re ction (C) Hopkins-Cole re ction (D) X nthoproteic re ction 176. Two min o groups re present in (A) Leucine (C) Lysine (B) Glut m te (D) Threonine 167. Prim ry structure of proteins c n e determined y the use of (A) Electroph oresis (C) Ninhydrin (B) Chrom togr phy (D) S nger’s re gent 168. Electrost tic onds c n e formed etween the side ch ins of (A) Al nine n d leucine (B) Leucine nd v line (C) Asp r te nd glut m te (D) Lysine nd sp r t te 169. S nger’s re gent cont ins (A) Phenylisothiocy n te (B) D nsyl chloride ( C) 1-Fluoro-2, 4-dinitro enzene (D) Ninhydrin 170. The most und nt protein in m mm ls is (A) Al umin (C) Coll gen (B) H emoglo in (D) El stin 177. During den tur tion of proteins, ll of the following re disrupted except (A) Prim ry structure (C) Terti ry structure (B) Second ry structure (D) Qu tern ry structure 178. All the following re r nched ch in Leucine (B) Al nine (D) V line
mino
cids except (A) Isoleucine (C)
179. An –OH group is present in the side ch in of (A) Serine (C) Lysine (B) Argini ne (D) Proline 180. Edm n’s re gent cont ins (A) Phenylisothiocy n te (B) 1-Fluoro-2, 4-dinitro e nzene (C) D nsyl Chloride (D) tBOC zide 171. Folding of newly synthesized proteins is ide isomer se (B) Prolyl cis-tr ns isomer se
cceler ted
y (A) Protein disulph
PROTEINS AND PROTEIN METABOLISM 181. Edm n’s re ction c n e used to (A) Determine the num er of tyrosine residues in protein (B) Determine the num er of rom tic mino cid residues in prot ein (C) Determine the mino cid sequence of protein (D) Hydrolyse the peptide onds in protein 182. Inherited deficiency of β− β−glucosidase causes (A) (B) (C) (D) Tay Sachs disease Metachromatic leukodystrophy Gaucher’s disease Multiple scleros is 37 190. Apolipoproteins C I, C II and C III are present in (A) Chylomicrons (C) HDL (B) VLDL (D) All of these 191. Apolipoprotiens C I, C II and C III are present in all of the following exc ept (A) Chylomicrons (C) LDL (B) VLDL (D) HDL 192. Apolipoprotein A I acts as (A) Enzyme activator (B) Ligand for receptor (C) Both (A) and (B) (D) None of these 193. Apolipoprotien B 100 acts as (A) Enzyme activator (B) Ligand for receptor (C) Both (A) and (B) (D) None of these 194. A polipoprotein C II is an activator of (A) Lecithin cholesterola acyl transferase (B) Phospholipase C (C) Extrahepatic lipoprotein lipase (D) Hepatic lipoprotein lipase 195. Nascent chylomicron receives apolipoproteins C and E from (A) VLDL remnant (C) LDL (B) VLDL (D) HDL 183. Tay Sachs disease results from inherited deficiency of (A) (B) (C) (D) Aryl sulphatase A Hexosaminidase A Sphingomyelinase Ceramidase 184. The largest alpolipoprotein is (A) Apo E (C) Apo B 100 (B) Apo B 48 (D) Apo A I 185. Apolipoprotein B 100 is synthesised in (A) Adipose tissue (C) Intestine (B) Liver (D) Liver and intestine 186. Apolipoprotein B 48 is synthesized in (A) Adipose tissue (C) Intestine (B) Liver (D) Liver and intestine 196. Terminal transferase (A) Removes nucleotides from 3’ end (B) Adds nucleotides at 3’ end (C) Removes nucleotides from 3’end (D) Adds nucleotides at 3’end 197. S1 nu clease hydrolyses (A) DNA of somatic cells (B) DNA of sperms (C) Any dou le stra nded DNA (D) Any single stranded DNA 198. Positive nitrogen alance is seen in ( A) Starvation (B) Wasting diseases (C) Growing age (D) Intestinal mala sorption 187. Apolipoproteins A I and A II are present in (A) (B) (C) (D) LDL only LDL an d VLDL HDL only HDL and chylomicrons 188. Apolipoprotein B 48 is present in (A) Chylomicrons (C) LDL (A) Chylomicrons (C) LDL only (B) VLDL (D) HDL (B) VLDL only (D) VLDL and LDL 189. Apolipoprotein B 100 is present in
38 199. Alanine can
Pyruv te
e synthesized from (A) (B) (C) (D) Glutamate and α-ketoglut r te
nd glut m te Pyruv te
nd
-ketoglut r te Asp r te
nd α-ketoglut r te
MCQs IN BIOCHEMISTRY 207. All the following st tement out hydroxyproline re true except (A) There is no codon for hydroxyproline (B) It is present in l rge mounts in coll gen (C ) Free proline c nnot e hydroxyl ted to hydroxyproline (D) Hydroxyl tion of pro line residues is c t lysed y dioxygen se 208. All of the following re requir ed for hydroxyl tion of proline residues except (A) Ascor ic cid (C) Ferrous io ns (B) Glut m te (D) Molecul r oxygen 200. All of the following re required for synthesis of l nine except (A) Pyruv te (C) Glut m te (B) α-ketoglut r te (D) Pyridox l phosph te 201. All of the following st tements out sp rt te re true except (A) It is n on-essenti l mino cid (B) It is dic r oxylic mino cid (C) It c n e synthe sized from pyruv te nd glut m te (D) It c n e converted into sp r gine 202. G lycine c n e synthesized from (A) Serine (C) Bet ine (B) Choline (D) All of the se 209. Cysteine c n e synthesized from methionine (B) Homoserine (D) Threonine
nd (A) Serine (C) Homocysteine
210. Methionine is synthesized in hum n ody from (A) (B) (C) (D) Cysteine omoserine Homocysteine nd serine Cysteine nd serine None of these 203. All of the following (C) (D) Glut m te Ammoni
nd h
re required for synthesis of glut mine except (A) (B) Pyridox l phosph te ATP
211. Hydroxyl tion of phenyl l nine requires ll of the following except (A) (B) (C) (D) Phenyl l nine hydroxyl se Tetr hydro iopterin NADH Molecul r oxygen 204. A coenzyme required for the synthesis of glycine from serine is (A) (B) (C) (D) ATP Pyridox l phosph te Tetr hydrofol te NAD 212. Non-Protein mino acid All of these
cids
re (A) (B) (C) (D) Ornithine β- l nine γ amino butyric
205. All of the followin statements about proline are true except (A) (B) (C) ( D) It is an imino acid It can be synthesized from lutamate It can be catabolise d to lutamate Free proline can be hydroxylated to hydroxyproline 213. The amino acid that under oes oxidative deamination at si nificant rate is (A) Alanine (C) Glutamate (B) Aspartate (D) Glutamine 206. A protein rich in hydroxyproline is (A) Prolamin (C) Colla en (B) Procolla en (D) Proinsulin 214. Allosteric inhibitor of (D) GMP
lutamate dehydro enase is (A) ATP (C) AMP (B) ADP
PROTEINS AND PROTEIN METABOLISM 215. Allsoteric activator of lutamate dehydro enase is (A) ATP (C) ADP and GDP (B) GTP (D) AMP and GMP 39 (A) Synthesis of carbamoyl phosphate and citrulline (B) Synthesis of citrulline and ar ininosuccinate (C) Synthesis of ar ininosuccinate and ar inine (D) Synthe sis of carbamoyl phosphate and ar ininosuccinate 224. Daily excretion of nitro e n by an adult man is about (A) 15–20 m (C) 5–10 m (B) 1.5–2 m (D) 15–20 m 216. Free ammonia is released durin (A) (B) (C) (D) Oxidative deamination of utamate Catabolism of purines Catabolism of pyrimidines All of these
l
217. An or an which is extremely sensitive to ammonia toxicity is (A) Liver (C) Kidney (B) Brain (D) Heart 225. Maple syrup urine diseases is an inborn error of metabolism of (A) (B) (C) (D) (A) (B) (C) (D) (A) (B) (C) (D) Sulphur containin amino acids Aromatic amin o acids Branched chain amino acids Dicarboxylic amino acids Metabolise cysteine Convert cystine into cysteine Incorporate cysteine into proteins Reabsorb cystin e in renal tubules Histidine carboxylase Histidine decarboxylase Histidase Histi dine oxidase 218. Ammonia is transported from muscles to liver mainly in the form of (A) Free ammonia (C) Aspara ine (B) Glutamine (C) Alanine 226. Cystinuria results from inability to 219. The major site of urea synthesis is (A) Brain (C) Liver (B) Kidneys (D) Mus cles 220. Carbamoyl phosphate required for urea synthesis is formed in (A) Cytosol (C ) Both (A) and (B) (B) Mitochondria (D) None of these 227. The defective enzyme in histidinemia is 221. Cytosolic and mitochondrial carbamoyl phosphate synthetase have the followi n similarity: (A) Both use ammonia as a substance (B) Both provide carbamoyl ph osphate for urea synthesis (C) Both require N acetyl lutamate as an activator (D ) Both are allosteric enzymes 222. The followin enzyme of urea cycle is present in cytosol: (A) (B) (C) (D) Ar ininosuccinic acid synthetase Ar ininosuccinase Ar inase All of these 228. All the followin statements about phenylketonuria are correct except (A) P henylalanine cannot be converted into tyrosine (B) Urinary excretion of phenylpy ruvate and phenyllactate is increased (C) It can be controlled by ivin a lowph enylalanine diet (D) It leads to decreased synthesis of thyroid hormones, catech olamines and melanin 229. All the followin statements about albinism are correc t except (A) Tyrosine hydroxylase (tyrosinase) is absent or deficient in melanoc ytes (B) Skin is hypopi mented (C) It results in mental retardation (D) Eyes are hypopi mented 223. ATP is required in followin
reactions of urea cycle:
40 230. Glycine is not required for the formation of (A) Taurocholic acid (C) Purin es (B) Creatine (D) Pyrimidines MCQs IN BIOCHEMISTRY themselves (C) When combined with some other lar e molecule, they can elicit an immune response (D) Once an immune response develops, the free hapten can be rec o nized by the antibody 238. Anti ens and haptens have the followin similarity: (A) They have hi h molecular wei hts (B) They can elicit immune response by the mselves (C) They can elicit an immune response only in association with some oth er lar e molecule (D) Once an immune response develops, free anti en and free ha pten can be reco nized by the antibody 239. The minimum number of polypeptide ch ains in an immuno lobulin is (A) Two (C) Five (B) Four (D) Six 231. Histamine is formed from histidine by (A) Deamination (C) Decarboxylation ( B) Dehydro enation (D) Carboxylation 232. DOPA is an intermediate in the synthesis of (A) (B) (C) (D) Thyroid hormone s Catecholamines Melanin Catecholamines and melanin 233. All the followin statements about pepsin are correct except (A) (B) (C) (D ) It is smaller than pepsino en It is formed by the action of HCl on its precurs or Its optimum pH is 1.0–2.0 It hydrolyses the C terminal and N terminal peptide b onds of proteins 240. Li ht chains of immuno lobulins are of followin types: (A) Alpha and kappa (B) Alpha and amma (C) Lambda and delta(D) Kappa and lambda 241 Immuno lobulin s are classified on the basis of (A) (B) (C) (D) Type of li ht chains Type of he avy chains Types of li ht and heavy chains Molecular wei ht 234. Pancreatic juice contains the precursors of all of the followin except (A) Trypsin (B) Chymotrypsin (C) Carboxypeptidase (D) Aminopeptidase 235. The only correct statement about chymotrypsin is (A) It is formed from trypsin (B) Carbox ypeptidase converts trypsin into chymotrypsin (C) Its optimum pH is around 7 (D) It hydrolyses peptide bonds involvin basic amino acids 236. The portion of the anti en molecule which is reco nized by antibody is known as (A) Hapten (C) Com plement (B) Epitope (D) Variable re ion 242. The molecular wei ht of li ht chains is (A) 10,000–15,000 (B) 20,000–25,000 (C) 25,000–50,000 (D) 50,000–75,000 243. The molecular wei ht of heavy chains is (A) 20 ,000–25,000 (B) 25,000–50,000 (C) 50,000–70,000 (D) 70,000–1,00,000 244. Secretory compo nent is present in (A) I A (C) I M (B) I G (D) All of these 237. All the followin statements about haptens are true except (A) They have hi h molecular wei hts (B) They cannot elicit an immune response by 245. The variable re ion of li ht chains is the (A) N terminal quarter (B) N ter minal half (C) C terminal quarter (D) C terminal half
PROTEINS AND PROTEIN METABOLISM 246. The variable re ion of li ht chain is the (A) (B) (C) (D) (A) (B) (C) (D) ( A) (B) (C) (D) N terminal quarter N terminal half C terminal quarter C terminal half One hypervariable re ion Two hypervariable re ions Three hypervariable re i ons Four hypervariable re ions One hypervariable re ion Two hypervariable re ion s Three hypervariable re ions Four hypervariable re ions 41 256. The immuno lobulin havin (D) I E
the lon est half life is (A) I A (C) I M (B) I G
257. The half life of I G is (A) 2–3 days (C) 8–10 days (B) 5–6 days (D) 20–25 days 247. The variable re ion of li ht chains has 258. Reco nition of anti en is the function of (A) (B) (C) (D) Variable re ion o f li ht chains Variable re ions of li ht and heavy chains Constant re ion of hea vy chains Constant re ions of li ht and heavy chains 248. The variable re ion of heavy chains has 259. The effector function of antibody is performed by (A) (B) (C) (D) Variable re ion of li ht chains Constant re ion of heavy chains Variable re ions of li ht and heavy chains Constant re ions of li ht and heavy chains 249. The most abundant immuno lobulin in plasma is (A) I A (C) I M (A) I A (C) I M (A) 1–5 m /dl (C) 60–500 m /dl (B) I G (D) I D (B) I G (D) I D (B) 40–200 m /dl (D) 700–1,500 m /dl 250. The lar est immuno lobulin is 260. Complement system can be activated by bindin (B) I D (D) I M
of anti en to (A) I A (C) I E
251. The plasma concentration of I A is 261. C1 component of classical complement pathway is made up of (A) (B) (C) (D) Complements 1q and 1r Complements 1q and 1s Complements 1r and 1s Complements 1q , 1r and 1s 252. An immuno lobulin found in exocrine secretions is (A) I A (C) I M (A) I A ( C) I D (B) I G (D) I E (B) I G (D) I E 253. Aller ic reactions are mediated by 262. The components of complement system are activated by (A) (B) (C) (D) Micros omal hydroxylation Phosphorylation Glycosylation Proteloysis 254. An immuno lobulin which can cross the placental barrier is (A) I A (C) I D 255. I M possesses (A) (B) (C) (D) Two li ht chains and two heavy chains Four li ht chains and four heavy chains Six li ht chains and six heavy chains Ten li ht chains and ten heavy chains (B) I M (D) None of these 263. The component system forms a membrane attack complex made up of (A) (B) (C) (D) Complements 1q, 1r and 1s Complements 1, 2, 3 and 4 Complements 5b, 6, 7 an d 8 Factors B and D
42 264. Factors B and D are required in (A) (B) (C) (D) The classical pathway of co mplement fixation The alternate complement pathway Both (A) and (B) None of thes e MCQs IN BIOCHEMISTRY 272. Gamma heavy chains are present in (A) I A (C) I M (B) I G (D) I D 273. Heavy chains in I D are of followin ) Epsilon
type: (A) Alpha (C) Delta (B) Gamma (D
265. The alternate complement pathway doesn’t involve (A) (B) (C) (D) Anti en anti body complex Complement 3 Factors B and D Membrane attack unit 274. On exposure to any anti en, the first antibody to be formed is of the follo win class: (A) I A (C) I M (B) I G (D) I E 266. Antibody diversity arises from (A) (B) (C) (D) Gene amplification Gene re a rran ement Alternative splicin All of these 275. Constant se ment enes of heavy chains are present in a cluster in which th e first ene on side is (A) Alpha (C) Delta (B) Gamma (D) None of these 267. A li ht chain ene is constructed from the followin se ments: (A) (B) (C) (D) Variable and constant se ments Variable, joinin and constant se ments Varia ble, diversity and constant se ments Variable, joinin , diversity and constant s e ments 276. Cell mediated immunity is the function of (A) B lymphocytes (C) Plasma cell s (B) T lymphocytes (D) Basophils 277. The most abundant T cells are (A) Cytotoxic T cells 278. T cells can reco n ise (A) Free anti ens (B) Anti ens bound to cells (C) Anti ens bound to antibodi es (D) Anti ens bound to MHC proteins 279. MHC proteins are unique to (A) Each c ell (C) Each individual (B) Each or an (D) Each species (B) Helper T cells (C) S uppressor T cells (D) Memory T cells 268. In metabolic point of view, amino acids are classified as (A) (B) (C) (D) G lyco enic Keto enic Glyco enic or Keto enic All of these 269. Diversity se ments are present in (A) (B) (C) (D) Li ht chain hain enes Li ht and heavy chain enes None of these
enes Heavy c
280. MHC class I proteins are present on the surface of (A) B cells only (B) T c ells only (C) Macropha es only(D) All cells 281. MHC class I proteins, in conjun ction with anti ens are reco nised by (A) Cytotoxic T cells (B) Helper T cells ( C) Suppressor T cells (D) Memory T cells 270. Constant se ments of heavy chains are of (A) Five types (C) Seven types (B) Six types (D) Ei ht types 271. Gamma heavy chains are of (A) Two types (C) Four types (B) Three types (D) Five types
PROTEINS AND PROTEIN METABOLISM 282. MHC class II proteins are present on the surface of (A) (B) (C) (D) All cel ls B lymphocytes only Macropha es only Macropha es and B lymphocytes 43 290. Human immunodeficiency virus destroys (A) Cytotoxic T cells (C) B cells (B) Helper T cells (D) Plasma cells 291. In aller ic diseases, the concentration of the followin is increased in pl asma: (A) I A (C) I D (A) Basophils (C) Both (A) and (B) (A) I A (C) I D (B) I G (D) I E (B) Mast cells (D) None of these (B) I G (D) I E 283. MHC Class II proteins, in conjunction with anti ens, are reco nised by (A) (B) (C) (D) Cytotoxic T cells Helper T cells Suppressor T cells Memory T cells 292. I E has a tendency to attach to 293. Rea inic antibody is 284. CD 8 is a transmembrane lycoprotein present in (A) (B) (C) (D) Cytotoxic T cells Helper T cells Suppressor T cells Memory T cells 294. Active immunity can be produced by administration of (A) (B) (C) (D) Killed bacteria or viruses Live attenuated bacteria or viruses Toxoids All of these 285. CD 4 is a transmembrane lycoprotein present in (A) Cytotoxic T cells (B) H elper T cells (C) Suppressor T cells (D) Memory T cells 286. CD 3 complex and p 56 present in (A) Cytotoxic T cells (C) Both (A) and (B) lck 295. Passive immunity can be produced by administration of (A) (B) (C) (D) Pure anti ens Immuno lobulins Toxoids Killed bacteria or viruses proteins are (B) Helper T cells (D) None of these 287. Cytotoxic T cells release (A) (B) (C) (D) Perforins Interleukins Colony sti mulatin factors Tumour necrosis factor 296. Helper T cells release all the followin except (A) (B) (C) (D) Interleukin s Colony stimulatin factors Perforins Tumour necrosis factor 288. Helper T cells release (A) (B) (C) (D) Interleukins Colony stimulatin ors Tumour necrosis factor All of these
fact
297. I G cleaved by papain into (A) Two li ht and two heavy chains (B) Two Fab a nd one Fc fra ments (C) Two pairs of one li ht and one heavy chain each (D) One Fab and two Fc fra ments 298. Bence Jones protein is (A) (B) (C) (D) An immuno l obulin A dimer of heavy chains A dimer of li ht chains A dimer of one heavy and one li ht chains 289. MHC Class III proteins include (A) (B) (C) (D) Immuno lobulins Components o f complement system T cells receptors CD4 and CD8 proteins
44 299. Bence Jones proteins possess all the followin properties except (A) They a re dimers of li ht chains (B) Their amino acids sequences are identical (C) Thei r N terminal halves have variable amino acid sequences (D) Their C terminal halv es have constant amino acid sequences 300. A Zwitterion is (A) Positive ion (B) Ne ative ion (C) Both (A) and (C) (D) None of these 301. After accountin for SD A, the net ain of ener y from 25 m of proteins is about (A) 70 kcal (C) 130 kc al (B) 100 kcal (D) 200 kcal MCQs IN BIOCHEMISTRY 306. The recommended ener y intake for an adult sedentary Indian man is (A) 1,90 0 kcal/day (C) 2,700 kcal/day (B) 2,400 kcal/day (D) 3,000 kcal/day 307. The recommended ener y intake for an adult sedentary Indian woman is (A) 1, 900 kcal/day (C) 2,400 kcal/day (B) 2,200 kcal/day (D) 2,700 kcal/day 308. Durin pre nancy, the followin should be added to the calculated ener y re quirement: (A) 300 kcal/day (C) 700 kcal/day (B) 500 kcal/day (D) 900 kcal/day 309. Durin first six months of lactation, the followin increment in ener y int ake is recommended: (A) 200 kcal/day (C) 550 kcal/day (B) 300 kcal/day (D) 1,000 kcal/day 302. After accountin for SDA, the net ain of ener y from 25 s is about (A) 70 kcal (C) 100 kcal (B) 95 kcal (D) 105 kcal
m of carbohydrate
310. The proximate principles of diet are (A) (B) (C) (D) Vitamins and minerals Proteins Carbohydrates and fats Carbohydrates, fats and proteins 303. After accountin for SDA, the net ain of ener y from 100 t (A) 600 kcal (C) 900 kcal (B) 780 kcal (D) 1020 kcal 311. The limitin Methionine
m of fat is abou
amino acid in wheat is (A) Leucine (C) Cysteine (B) Lysine (D)
304. If proteins, carbohydrates and fats are consumed to ether: (A) The total SD A is the sum of individual SDAs of proteins, carbohydrates and fats (B) The tota l SDA is more than the sum of individual SDAs of proteins, carbohydrates and fat s (C) Carbohydrates and fats lower the SDA of proteins (D) Proteins raise the SD A of carbohydrates and fats 305. After calculatin the ener y requirement of a p erson: (A) (B) (C) (D) 10% kcal are subtracted on account of SDA 10% kcal are ad ded on account of SDA 20% kcal are subtracted on account of SDA 20% kcal are sub tracted on account of SDA 312. The limitin amino acid in pulses is (A) Leucine (C) Tryptophan 313. Maize is poor in (A) (B) (C) (D) Lysine Methionine Tryptophan Lysine and tryptophan (B ) Lysine (D) Methionine 314. The percenta e of in ested protein/ nitro en absorbed into blood stream is known as (A) (B) (C) (D) Net protein utilisation Protein efficiency ratio Di est ibility coefficient Biolo ical value of protein
PROTEINS AND PROTEIN METABOLISM 315. Biolo ical value of a protein is (A) The percenta e of in ested protein/nit ro en absorbed into circulation (B) The percenta e of in ested protein/nitro en in the body (C) The percenta e of in ested protein utilised for protein synthesi s in the body (D) The ain in body wei ht ( m) per m of protein in ested 316. N et protein utilisation depends upon (A) Protein efficiency ratio (B) Di estibili ty coefficient (C) Di estibility coefficient and protein efficiency ratio (D) Di estibility coefficient and biolo ical value 317. The ain in body wei ht ( m) p er m of protein in ested is known as (A) (B) (C) (D) Net protein utilisation Pr otein efficiency ratio Di estibility coefficient Biolo ical value of protein 323 . Protein content of meat is about (A) 10% (C) 16% (B) 13% (D) 20% 45 324. Protein content of rice is about (A) 7% (C) 15% (B) 12% (D) 20% 325. The calorific value of wheat is about (A) 2.5 kcal/ m (C) 4.5 kcal/ m (B) 3 .5 kcal/ m (D) 5.5 kcal/ m 326. For ve etarians, pulses are an important source of (A) Carbohydrates (C) Fa t (B) Proteins (D) Iron 327. The amino acids present in pulses can supplement the limitin f (A) Cereals (C) Fish (B) Milk (D) Nuts and beans
amino acids o
328. Milk is a ood source of (A) (B) (C) (D) Proteins, calcium and iron Protein s, calcium and ascorbic acid Proteins, lactose and retinol Proteins, lactose and essential fatty acids 318. The followin is considered as reference standard for comparin onal quality of proteins: (A) Milk proteins (C) Meat proteins (B) E D) Fish proteins
the nutriti proteins (
329. Milk is a ood source of all of the followin except (A) (B) (C) (D) Essent ial amino acids Vitamin C Galactose Calcium and phosphorous 319. Biolo ical value of e %
proteins is about (A) 70 % (C) 86 % (B) 80 % (D) 94
320. The followin has the hi hest protein efficiency ratio: (A) Milk proteins ( C) Meat proteins (B) E proteins (D) Fish proteins 330. Milk is poor in (A) Cholesterol (C) Calcium (B) Retinol (D) Iron 321. The followin has the lowest protein efficiency ratio: (A) Maize proteins ( C) Milk proteins (B) Wheat proteins (D) Rice proteins 331. E is rich in all of the followin d (B) Saturated fatty acids (D) Calcium 332. A phosphoprotein present in e D) Ovovitellin 322. Protein content of e
except (A) Cholesterol (C) Ascorbic aci
is (A) Casein (C) Ovo lobulin (B) Albumin (
is about (A) 10% (C) 16% (B) 13% (D) 20%
46 333. Consumption of raw e s can cause deficiency of (A) Calcium (C) Biotin 334. E is poor in (A) (B) (C) (D) Essential amino acids Carbohydrates Avidin Bioti n (B) Lipoic acid (D) Vitamin A MCQs IN BIOCHEMISTRY (C) Muscle wastin occurs in marasmus but not kwashiorkor (D) Subcutaneous fat d isappears in marasmus but not in kwashiorkor 342. Ener y reserves of an avera e well fed adult man are about (A) 50,000 kcal (C) 200,000 kcal (B) 100,000 kcal ( D) 300,000 kcal 335. Cholesterol is present in all the followin B) Fish (D) E yolk
except (A) Milk (C) E
white (
343. Durin starvation, the first reserve nutrient to be depleted is (A) Glyco e n (C) Tri lycerides (B) Proteins (D) Cholesterol 336. Meat is rich in all of the followin e (D) Zinc
except (A) Iron (C) Copper (B) Fluorin
344. Synthesis of the followin enzymes is increased durin starvation. (A) (B) (C) (D) Di estive enzymes Gluconeo enic enzymes Urea cycle enzymes Glucokinase 337. Kwashiorkor occurs when the diet is severely deficient in (A) Iron (C) Prot eins (B) Calories (D) Essential fatty acids 345. In hypoparathyroidism (A) Plasma calcium and inor anic phosphorous are low (B) Plasma calcium and inor anic phosphorous are hi h (C) Plasma calcium is low and inor anic phosphorous hi h (D) Plasma calcium is hi h and inor anic phosphor ous low 346. The number of amino acid residues in calcitonin in (A) 9 (C) 51 (B) 32 (D) 84 338. Clinical features of Kwashiorkor include all of the followin except (A) Me ntal retardation (B) Muscle wastin (C) Oedema (D) Anaemia 339. Kwashiorkor usua lly occurs in (A) (B) (C) (D) The post weanin period Pre nancy Lactation Old a e 340. Marasmus occurs from deficient intake of (A) (B) (C) (D) Essential amino ac ids Essential fatty acids Calories Zinc 347. Calcitonin is synthesised in (A) (B) (C) (D) Parathyroid nd Pars intermedia of pituitary Adrenal cortex
lands Thyroid
la
341. Marasmus differs from Kwashiorkor in the which of these followin respect ( A) Mental retardation occurs in kwashiorkor but not in marasmus (B) Growth is re tarded in kwashiorkor but not in marasmus 348. Plasma calcium is lowered by (A) Parathormone (C) Aldosterone (B) Calcitoni n (D) Deoxycorticosterone
PROTEINS AND PROTEIN METABOLISM 349. α Cells of Islets of L ngerh ns secrete (A) Insulin (C) Som tost tin 350. A/G r tio is (A) (B) (C) (D) Strength of proteins r tio of serum proteins r tio of cerulopl smin None of these (B) Gluc gon (D) Cholecystokinin 357. G strin stimul tes (A) G stric motility (C) Both (A) nd (B) 47 (B) G stric secretion (D) None of these 358. Secretin is m de up of (A) 17 cids (D) 47 mino cids
mino
cids (C) 37
mino
cids (B) 27
mino
359. Secretin c uses ll of the following except (A) (B) (C) (D) Secretion of p ncre tic juice Secretion of ile Inhi ition of g stric secretion Stimul tion of intestin l motility 351. Insulin is m de up of (A) A single polypeptide ch in h ving 51 mino cid r esidues (B) A single polypeptide ch in h ving 84 mino cid residues (C) A-ch in h ving 21 nd B-ch in h ving 30 mino cid residues (D) A-ch in h ving 30 nd B -ch in h ving 21 mino cid residues 352. The num er of mino cid residues in p reproinsulin is (A) 51 (C) 109 (B) 84 (D) 119 360. All of the following st tements out cholecystokinin p ncreozymin re true except (A) It is secreted y mucos of sm ll intestine (B) It stimul tes secret ion of p ncre tic juice rich in enzymes (C) It stimul tes contr ction of g ll l dder (D) It inhi its g stric motility 361. All of the following st tements ou t p ncre tic som tost in re true except (A) (B) (C) (D) It is secreted y δ cells of islets of Langerhans It stimulates the secretion of gastrin It inhibits the secretion of secretin It inhibits the secretion of cholecystokininpancreozymin 353. Pre-proinsulin contains a signal sequence having (A) (B) (C) (D) 9 amino ac i resi ues 19 amino aci resi ues 27 amino aci resi ues 33 amino aci resi ues 354. The number of intra-chain isulphi e bon s in pro-insulin: (A) One (C) Thre e 355. Pentagastrin is a (A) (B) (C) (D) Naturally occurring form of gastrin Ina ctive metabolite of gastrin Active metabolite of gastrin Synthetic form of gastr in (B) Two (D) Four 362. Histi ine is converte into histamine by (A) Carboxylation (C) Methylation (B) Decarboxylation (D) Hy roxylation 363. Histamine is synthesise of these
in (A) Brain (C) Basophils (B) Mast cells (D) All
364. Histamine causes all the following except (A) (B) (C) (D) Stimulation of ga stric secretion Vasoconstriction Pruritus Increase in capillary permeability 356. Secretion of gastrin is evoke by (A) (B) (C) (D) Entry of foo h Vagal stimulation Lower aliphatic alcohols All of these 365. H2-receptors are blocke ine (D) Cimeti ine
into stomac
by (A) Diphenhy ramine (B) Mepayramine (C) Pyrilam
48 366. Serotonin is synthesise Tryptophan
from (A) Serine (C) Tyrosine (B) Phenylalanine (D)
MCQs IN BIOCHEMISTRY 375. The most abun ant protein in bones is (A) (B) (C) (D) Collagen type I Colla gen type II Collagen type III Non-collagen proteins 367. All the following statements about serotonin are true except (A) (B) (C) (D ) It causes vasoli atation It causes bronchoconstriction It is metabolize by mo noamine oxi ase Its metabolite is 5-hy roxyin ole acetic aci 376. The most abun ant collagen in cartilages is (A) Type I (C) Type III (B) Typ e II (D) Type IV 368. All the following statements about angiotensin are true except (A) (B) (C) (D) (A) (B) (C) (D) Its precursor is an α2-glo ulin Its ctive form is n oct pept ide It is v sodil tor It incre ses the secretion of ldosterone Inhi iting the synthesis of c techol mines Ant gonising the ction of ldosterone Stimul ting the rele se of renin Inhi iting the re kdown of ngiotensin
377. Coll gen nd el stin h ve the following simil rity: (A) (B) (C) (D) Both r e triple helices Both h ve hydroxyproline residues Both h ve hydrolysine residue s Both re glycoproteins 369. Methyl dop
decre ses
lood pressure
y
378. A norm l coll gen structure is seen in ll of the following except (A) (B) (C) (D) I-cell dise se Osteogenesis imperfect Menke’s dise se Ehlers-D nlos sydro me 370. Binding of g mm - mino utyric cid to its receptors in r in incre ses the perme ility of cell mem r ne to (A) Cl– (C) K + (B) N + (D) C ++ 379. I-cell dise se results from sence of the following from lysosom l enzymes : (A) (B) (C) (D) (A) (B) (C) (D) Sign l sequence M nnose-6-phosph te Si lic ci d A serine residue Are not synthesised Are in ctive L ck sign l sequence C nnot re ch lysosomes 371. Binding of cetylcholine to its receptors incre ses the perme l mem r ne to (A) C ++ (C) K + (B) N + (D) N + nd K+
ility of cel
380. In I-cell dise se, lysosom l enzymes 372. All of the following re glycoproteins except (A) Coll gen (C) Tr nsferrin (A) Proteoglyc ns (C) Both (A) nd (B) (A) (B) (C) (D) (B) Al umin (D) IgM (B) G lycoproteins (D) None of these 381. Ren l glycosuri occurs due to (A) Incre sed filtr tion of glucose in glome ruli (B) Incre sed secretion of glucose y ren l tu ul r cells (C) Decre sed re sorption of glucose y ren l tu ul r cells (D) Incre sed conversion of glycogen into glucose in tu ul r cells 382. H em turi c n occur in (A) H emolytic n em i 373. Si lic
cids
re present in
374. Hy luronid se hydrolyses Hy luronic ronic cid nd chondroitin sulph te
cid Chondroitin sulph te Hep rin Hy lu
PROTEINS AND PROTEIN METABOLISM (B) Mism tched lood tr nsfusion (C) Yellow fever (D) Stone in urin ry tr ct 383 . H em turi c n occur in ll of the following except (A) (B) (C) (D) Acute glom erulonephritis C ncer of urin ry tr ct Stone in urin ry tr ct Mism tched lood t r nsfusion hours nd, then, me sure (A) (B) (C) (D) Serum ure Serum cre tinine Urine output in one hour Specific gr vity of urine 49 393. Among the following, the most sensitive indic tor of glomerul r function is (A) (B) (C) (D) Serum ure Serum cre tinine Ure cle r nce Cre tinine cle r nce 384. Chyluri c n e detected y ddition of the following to the urine: (A) Sul phos licylic cid (B) Nitric cid (C) Acetic nhydride (D) Chloroform 385. Norm l r nge of serum ure is (A) 0.6–1.5 mg/dl (C) 20–45 mg/dl (A) 0.6–1.5 mg/dl (C) 20–45 m g/dl (A) 54 ml/min (C) 110 ml/min (A) 54 ml/min (C) 110 ml/min (B) 9–11 mg/dl (D) 60–100 mg/dl (B) 9–11 mg/dl (D) 60–100 mg/dl (B) 75 ml/min (D) 130 ml/min (B) 75 ml/mi n (D) 130 ml/min 394. All the following st tements out inulin re correct except (A) (B) (C) (D ) It is completely non-toxic It is completely filtered y glomeruli It is not re sor ed y tu ul r cells It is secreted y tu ul r cells 386. Norm l r nge of serum cre tinine is 395. Non-protein nitrogenous su st nces in lood include cept (A) Ure (C) Cre tinine (B) Uric cid (D) Inositol 387. St nd rd ure 388. M ximum ure
ll of the following ex
cle r nce is cle r nce is
396. Non-protein nitrogenous su st nces in lood re r ised in (A) (B) (C) (D) S t rv tion Liver d m ge Ren l f ilure All of these 389. Aver ge cre tinine cle r nce in ml/min (B) 75 ml/min (D) 130 ml/min
n
dult m n is
out (A) 54 ml/min (C) 110
397. Cre tinine cle r nce is dece sed in (A) (B) (C) (D) Acute tu ul r necrosis Acute glomerulonephritis Hypertension Myop thies 390. Inulin cle r nce in n ver ge min (B) 75 ml/min (D) 130 ml/min
dult m n is
out (A) 54 ml/min (C) 110 ml/
398. Serum myl se is incre sed in (B) Acute p ncre titis (A) Acute p rotitis (C ) P ncre tic c ncer (D) All of these 399. M ximum rise in serum myl se occurs i n (A) (B) (C) (D) Acute p rotitis Acute p ncre titis Chronic p ncre titis P ncre tic c ncer Q391. Among the following, test of tu ul r function is (A) (B) (C) (D) Cre tin ine cle r nce Inulin cle r nce PAH cle r nce PSP excretion test 392. A simple w y to 2
ssess tu ul r function is to withhold food
nd w ter for 1
50 400. Serum lip se is incre sed in (A) Acute p rotitis (B) Acute p ncre titis (C) Infective hep titis (D) Bili ry o struction 401. Which one of the following met olites is not directly produced in the hexose monophosph te p thw y? (A) (B) ( C) (D) Fructose-6-phosph te Dihydroxy cetone phosph te CO2 Erythrose-4-phosph t e MCQs IN BIOCHEMISTRY (B) Must e supplied in the diet ec use the hum n h s n imp ired ility to sy nthesize the c r on ch in of the corresponding keto cids (C) Are identic l in l l species studied (D) Are defined s those mino cids which c nnot e synthesiz ed y the org nism t r te dequ te to meet met olic requirements 408. Which mong the following is n essenti l mino cid? (A) Cysteine (C) Tyrosine (B) Le ucine (D) Asp rtic cid 402. Which one of the following st tements concerning glucose-6-phosph te dehydr ogen se deficiency is correct? (A) Young R.B.Cs, p rticul rly reticulocytes, con t in the highest enzyme ctivity cells show less enzyme ctivity (B) Glucose-6-P Dehydroglucose deficiency le ds to disfuction of m ny tissues (C) G-6-p Dehydro glucose deficiency is due to single deletion of l rge sequence of DNA in the G-6-PD gene (D) G-6-PD deficiency is precipit ted y ingestion of drugs such s spirin 403. The phenomenon of inhi ition of glycolysis y O2 is termed s (A) Red drop (C) Mich elis effect (B) P steur effect (D) Fischer’s effect 409. Which mong the following is (B) Arginine (D) Al nine
sic
mino
cid? (A) Asp rgine (C) Proline
410. This mino cid c nnot h ve optic l isomers: (A) Al nine (C) Threonine (B) Histidine (D) Glycine 411. The mino cid which cont ins line (B) Arginine (D) Ornithine
gu nidine group is (A) Histidine (C) Citrul
412. GABA(g m mino utyric cid) is (A) (B) (C) (D) Post-syn ptic excit tory t r nsmitter Post-syn ptic inhi itor tr nsmitter ctiv tor of gli -cell function i nhi itor of gli -cell function 404. Ser tonin is derived in the ody from the following l nine (C) Tryptoph n (B) Histidine (D) Serine
mino
cid: (A) Phenyl
413. Sulphur-cont ining mino cid is (A) Glut thione (C) Homocysteine (B) Chond roitin sulph te (D) Tryptoph n 405. Which mino cid is ine (D) Methionine
lipotropic f ctor? (A) Lysine (C) Tryptoph n (B) Leuc
414. The useful re gent for detection of mino cids is (A) (B) (C) (D) Molisch re gent Dichlorophenol Indophenol Ninhydrin Biuret 406. Which mong the following is nutrition lly essenti l (A) Al nine (C) Tyrosine (B) Glycine (D) Tryptoph n
mino
cid for m n ?
407. The essenti l mino cids (A) Must e supplied in the diet ec use the org nism h s lost the c p city to min te the corresponding keto cids 415. The mino cid which cont ins (B) Arginine (D) Tryptoph n
n indole group is (A) Histidine (C) Glycine
PROTEINS AND PROTEIN METABOLISM 416. S k guchi re ction is nswered y (A) (B) (C) (D) Lysine Ornithine Arginine Arginino succinic cid (A) Lysine (C) Serine (B) Glut mine (D) Citrulline 51 425. An mino cid which cont ins ne (B) Methionine (D) Cystine
disulphide
ond is (A) Lysine (C) Homocystei
417. The pH of n mino cid depends (A) Optic l rot tion (B) Dissoci tion const nt (C) Diffusion coefficient(D) Ch in length 418. When mino cids re tre ted with neutr l form ldehyde, the pH of the mixture (A) (B) (C) (D) Is not ltered Incre ses Decre ses First incre ses then decre ses 426. One of the following h s phenolic group: (A) Histidine (C) Ser tonine (B) Hydroxy lysine (D) Hydroxy proline 427. An mino cid not cont ining the usu l— COOH group is (A) Al nine (C) Methion ine (B) Tryptoph n (D) T urine 419. Which mong the following h s n imid zole group? (A) Histidine (C) Proline (B) Tryptoph n (D) Hydroxy proline 428. Br nched ch in mino cids re (A) (B) (C) (D) Cysteine nd cystine Tyrosin e nd Tryptoph n Glycine nd Serine V line, Leucine nd Isoleucine 420. The mino cid exist s Zwitter ions when they re in (A) solid st te (C) lk line solution (A) S lting out (C) Flourimetry (A) (B) (C) (D) (B) cidic solu tion (D) neutr l solution (B) Electrophoresis (D) Both (A) nd (B) 429. A Zwitter ion is one which h s in queous solution: (A) One positive ch rge nd one neg tive ch rge (B) Two positive ch rges nd one neg tive ch rge (C) Tw o neg tive ch rges nd one positive ch rge (D) No electric l ch rges t ll 430. The mino cid which gives yellow colour with Ninhydrin in p per chrom togr phy is (A) Tyrosine (C) Tryptoph n (B) Proline (D) Al nine 421. Pl sm
proteins
re isol ted
y
422. After digestion mino cids Are sor ed into port l circul tion Are sor ed into lymph Are excreted to the extent of 50% Converted into glucose in the in testine 423. Cysteine h s the formul : (A) CH3SH (B) H2N—CH2—COOH (C) HS—CH2—CH(NH2)—COOH (D) S—CH (NH2)—COOH S—CH2—CH(NH2)—COOH 424. The compound h ving the formul H2N—CO—NH—CH2—CH2—CH2 NH2 431. Hydroxyl tion of Proline nd Lysine in protein is effected y (A) Vit min B1 (C) Vit min B6 (A) Tyrosine (C) Proline (B) Vit min B2 (D) Vit min C 432. Millon’s test is for identific tion of (B) Tryptoph n (D) Arginine 433. Hopkins-Cole test is for identific tion of (A) Tyrosine (C) Arginine (B) Tr yptoph n (D) Cysteine
52 434. Coll gen is very rich in (A) Glycine (C) Asp rtic mic cid MCQs IN BIOCHEMISTRY 445. The m jor end product of protein nitrogen met (C) Ure (B) Uric cid (D) NH3 435. All mino cids erine (D) Tryptoph n
re optic lly
cid (B) Serine (D) Glut
olism in m n is (A) Glycine
ctive except (A) Glycine (C) Threonine (B) S
446. An mino cid not involved in ure Histidine (D) Citrulline
cycle is (A) Arginine (C) Ornithine (B)
436. Out of 200 different mino cids form in n ture the num er of resent in protein: (A) 20 (C) 40 (B) 25 (D) 35 447. NH3 is detoxified in (D) Glut mine
r in chiefly
s (A) Ure
438. The ioniz
le groups of
mino
cids
re
cids p
(C) Cre tinine (B) Uric
437. Enzyme c t lyzed hydrolysis of proteins produces A) D (C) DL (B) L (D) All of these 448. In hum ns, NH3 is detoxified in liver cid (D) Uronic cid
mino
mino
cid
cids of the form: (
s (A) Cre tinine (C) Ure
(B) Uric
t le st. (A) 1 (C) 3 (B) 2 (D) 4
449. The ody protein fter eighteen ye rs (A) Rem ins unch nged (B) Is decompos ed only slightly t interv ls of one month (C) Is in const nt st te of flux (D ) Is used only for energy requirement 450. The only known physiologic l methyl t ing gents in the nim l org nism re (A) Choline nd et ine (B) Choline nd δ-a enosyl methionine (C) Betaine an δ-a enyosyl methionine (D) Dimehtyl glycine an betaine 451. In the synthesis of 1 molecule of urea in the Kreb’s Hanseleit cycle, the number of ATPs require is (A) 1 (C) 3 (B) 2 (D) 4 439. The neutral amino aci
is (A) Lysine (C) Leucine (B) Proline (D) Histi ine
440. The amino aci containing hy roxyl group: (A) Alanine (C) Arginine (B) Isol eucine (D) Threonine 441. The sulphur containing amino aci : (A) Homoserine (C) Methionine (B) Serine (D) Valine 442. The basic amino aci : (A) Glycine (C) Histi ine (B) Leucine (D) Proline 452. For biosynthesis of proteins (A) Amino aci s only are require (B) Amino ac i s an nucleic aci s only are require (C) Amino aci , nucleic aci s an ATP on ly are require (D) Amino aci s, nucleic aci s, ATP, GTP, enzymes an activators are require 443. The amino aci which synthesizes many hormones: (A) Valine (C) Alanine (A) Acetic aci (C) Ethanol (B) Phenyl alanine (D) Histi ine (B) Chloroform (D) Benz ene 444. Amino aci s are insoluble in
PROTEINS AND PROTEIN METABOLISM 453. Transmethylation of guani o acetic aci gives (A) (B) (C) (D) Creatine phos phate Creatinine Choline n-methyl nicotinami e 53 461. The first amino aci incorporate in a polypepti e in a ribosome of a human is (A) N formyl methionine (C) Phenyl alanine (B) Methionine (D) Hy roxy lysine 454. The 2 energy rich compoun s nee e for protein biosynthesis are (A) ATP an GTP (C) ATP an CTP (B) ATP an UTP (D) ATP an TTP 462. The first amino aci incorporate in a polypepti e in a ribosome of a bacte rium is (A) N formyl methionine (C) Alamine (B) Methionine (D) Glycine 455. The following ketoaci is involve in fixing ietary NH3 into amino aci : ( A) Pyruvate (C) Oxalo succinate (B) Oxalo acetate (D) α-keto glut r te
463. The integr tor etween the TCA cycle nd ure uv te (A) (B) (C) (D) (B) M l te (D) Citr te
cycle is (A) Fum r te (C) Pyr
464. Bence jones proteinuri l ch r cterized y Non-he t co gul ility He t co gu l ility t 100°C He t co gul ility t 45 to 60°C Precipit tion t 25°C 456. The met olite which sust ins ure cycle is (A) (B) (C) (D) Ornithine Citru lline C r moyl phosph te n- cetyl glut m te 465. Bence Jones proteins m y e excreted in urine of p tients suffering from (A ) Tu erculosis (B) Di etes mellitus (C) Multiple myelom (D) Hyperthyroidism 46 6. X nthuric cid is n norm l met olite of (A) X nthine (C) Tyrosine (B) Uri c cid (D) Tryptoph n 457. Tetr hydroglol te c n e freed from N5 methyl tetr hydrofol te only y (A) Nor epinephrine (B) Eth nol mine (C) Nicotin mide (D) Vit min B12 458. Neogene sis of methyl group is (A) The v il ility of methyl group form δ a enosyl methio nine (B) The availability of methyl group from betaine (C) Interaction between N 5 N10 methylene tetra hy rofolate with a NAD + epen ent re uctase (D) Availabil ity of methyl group from methyl B12 459. More creatinine is excrete by (A) A ul t males (C) Chil ren (B) A ult females (D) Pregnant women 467. Two nitrogen atoms of Urea in the urea cycle come from (A) (B) (C) (D) NH3 One from NH3 an one from aspartate One from NH3 an one from glutamate One from NH3 an one from alanine 468. Pyruvic aci can be obtaine by transamination of alanine with (A) (B) (C) (D) α- keto glut ric cid Aceto cetic cid −OH butyric acid Phosphoenol Pyruvic acid
460. A rowin peptide in a ribosome can not be shifted to the adjacent ribosome because (A) It is firmly attached (B) It will et the amino acid cleaved (C) Th e ap between the ribosomes is too bi for a shift (D) The adjacent ribosomes ha ve different composition 469. In the synthesis of 1 molecule of urea in the Kreb’s Henseleit cycle the numb er of AMPs formed is (A) 1 (C) 3 (B) 2 (D) 4
54 470. Formation of melanin from tyrosine requires the action of (A) (B) (C) (D) D opa decarboxylation Diamine oxidase Peroxidase Tyrosinase MCQs IN BIOCHEMISTRY (B) H2N—Gly—Ar —Lys—Phe—COOH + Asp (C) H2N—Ar —Lys—Phe—Asp—COOH + Gly (D) H2N—Gly—Ar —Lys— 478. Which of the followin techniques is used to separate proteins based upon differences in their mass? (A) (B) (C) (D) Isoelectric focusin Dialysis SDS el Electrophoresis Western blottin 471. (A) 472. ycle
In one of the followin the quality of the protein synthesized is affected: Diabetes mellitus (B) Gont (C) Multiple myeloma (D) Primaquine sensitivity Citrulline is an intermediate of (A) TCA cycle (C) Pentose cycle (B) Urea c (D) Calvin cycle
473. The semialdehydes are formed under the action of enzymes characterised as ( A) (B) (C) (D) Aldolases Peptidyl lysyl oxidases Colla enases Elastases 479. The reatest bufferin capacity at physiolo ic pH would be provided by a pr otein rich in which of the followin amino acids ? (A) Lysine (C) Aspartic acid (B) Histidine (D) Valine 480. Which one of the amino acids could serve as the best buffer at pH 7? (A) Gl utamic acid (C) Valine (B) Ar inine (D) Histidine 474. Which of the followin statement about the peptide bond is true? (A) (B) (C ) (D) It is a carbon carbon bond It has cis hydro en and oxy en roups It is pla nar It has rotational freedom 481. Which one of the followin statements concernin lutamine is correct? (A) (B) (C) (D) Contains three tetratable roups Is classified as an acidic amino ac id Contains an amide roup Mi rates to the cathode durin electrophoresis at pH 7.0 475. Isoenzymes for a iven reaction (A) (B) (C) (D) Have different spedificitie s Have identical affinities for the same substrate Exhibit different electrophor etic motilities Contain similar ratios of different polypeptide chains 482. One of the iven example is an amino acid: (A) Oh Lysine (C) Leucine (B) Pr otein (D) Serine 476. The hi hest concentration of cystine can be found in (A) Melanin (C) Myosin (B) Chondroitin sulphate (D) Keratin 483. The lone pair of electrons at one of the rin nitro ens in the iven amino acid makes a potential li and, which is important in bindin the iron atoms in h emo lobin: (A) Tryptophan (C) Histidine (B) Threonine (D) Serine 477. One round of Edman de radation of the peptide: H 2 N— Gly—Ar —Lys—Phe— Asp— COOH woul result in which of the followin structures or their phenyl isothiocyanate deriv atives? (A) H 2N—Gly—Ar —COOH + H2N—Lys— Phe— Asp—COOH 484. The amino acid which is not optically active is (A) Alanine (C) Glutamine ( B) Glycine (D) Lysine
PROTEINS AND PROTEIN METABOLISM 485. Optically active compounds are capable of (A) (B) (C) (D) Different reactio ns Rotatin plane of polarized li ht Showin same chemical properties None of th ese 55 494. In prehepatic jaundice, protein flocculation test is (A) (B) (C) (D) Normal /weekly positive Usually positive Ne ative None of these 486. The reference compound for absolute confi uration of optically active compo und is (A) Alanine (C) Glyceraldehyde (B) Lactic acid (D) Dihydroxy acetone 495. Side chains of all amino acids contain aromatic rin s except (A) Pheynl ala nine (C) Tyrosine (B) Alanine (D) Tryptophan 487. All the standard amino acids except the followin have one chiral ‘c’ atom: (A) (B) (C) (D) (A) (B) (C) (D) Threonine, Isoleucine Isoleucine, Alanine Threonine , Alanine Alanine, Glutamine Defense Helps immunity of the body Not predicatable None of these 496. In Nitroprusside test, amino acid cystein produces (A) (B) (C) (D) Blue col our complex Red colour Yellow colour Purple colour 488. The role of complement proteins: 497. Bonds that are formed between two cysteine residues is (A) Disulphide (C) E lectrostatic (B) Peptide (D) Hydrophobic 489. Optical isomers that are mirror ima es and non superimposable are called (A ) Diastereomers (C) dl isomers (B) Euantiomers (D) Stereomers 498. The acid amide of Aspartic acid is (A) Glutamine (C) Aspar ine (B) Ar inine (D) Ornithine 490. Livin cells have the unique ability to synthesize only _________ the form of optical isomer due to _________. (A) (B) (C) (D) ‘d’ form, stereospecific enzymes ‘l’ form stereospecific enzymes ‘d’ form, DNA ‘L’ form, DNA 499. It is the only amino acid havin an ionizin ‘R’ roup with a pK’ near 7 and is i mportant in the active site of some enzymes: (A) Ar inine (C) Cystine (B) Cystei n (D) Histidine 491. Isoelectric pH of an amino acid is that pH at which it has a (A) Positive c har e (C) No net char e (A) Albumin (C) Both A and B (B) Ne ative char e (D) All of these (B) Globulin (D) None of these 500. Hemo lobin has a hi h content of this amino acid: (A) Proline (C) Ar inine (B) Leucine (D) Histicline 492. Albuminoids are similar to 501. A hexa peptide with 5 aspartic acid would have a net char e at pH 7: (A) Ne utral (C) Ne ative (B) Positive (D) Not predictable 493. Abnormal chain of amino acids in sickle cells anaemia is (A) Alpha chain (C ) Gama chain (B) Beta chain (D) Delta chain 502. In the enetic disorder of cystinuria, the patient excretes lar e quantitie s of cystine in their urine and its low solubility causes crystalline cystine to precipitate as stones in kidneys. The remedy involves
56 in estin Na HCO 3. Reaction of this treatment is (A) NaHCO2 combines with cysti ne (B) NaHCO3 raises the pH above the isoelectric point of cystine (C) NaHCO 3 p revents stone formation by hydrolysis of cystine to cysteine (D) None of these 5 03. In the followin reaction, Alanine acts as a MCQs IN BIOCHEMISTRY 508. The amino acid which has a pK near 4 and thus is ne atively char ed at pH 7 is (A) Alanine (C) Glutamine (B) Glutamic acid (D) Aspar ine 509. The side chain of which of the followin amino acid contain sulphur atom? ( A) Methionine (C) Leucine (B) Threonine (D) Tryptophan 510. Which of the followin s ives a positive test for Ninhydrin? (A) Reducin s u ars (B) Tri lycerides (C) Alpha aminoacids (D) Esterified Fats 511. In lutath ione (a tripeptide) is present apart from Glutamic acid and cysteine: (A) Serine (C) Leucine (A) Glycine (C) Valine (B) Glycine (D) Phenyl alanine (B) Alanine ( D) Serine H H + + H 3 N – C – COO ——→ H 3 N – C – COOH CH 3 CH 3 (A) Acid (C) Zwitter ion (B) B se (D) None of these 504. Amino cids excepting histidine re not good uffering gents in cell ec u se (A) They exist s zwitter ions (B) Their pk nd not in the physiologic l pH o f cell (C) Only Histidine h s pk of its R group t 6.0 unlike the others which h ve t different pH (D) None of these 505. At neutr l pH Al nine h s the fol lowing structure: (A) 512. 2 -Amino 3-OH prop noic
cid is
513. All mino cids h ve one symmetric c r on stidine (B) Asp rgine (D) Glycine 514. Num er of mino cids present in the pl nt, (A) 20 (C) 150 (A) (B) (C) (D) (B) 80 (D) 200
tom, except (A) Arginine (C) Hi nim l
nd micro i l proteins:
H H + H2N − C − COOH (B) H3 N − C − COO CH3 CH3 H H2 N − C − COO (D) CH3 H + H2 N − C − CO 515. Immuno lobulins are characterized by their Heavy chains Molecular wei ht Li ht chains Electrophoretic behaviour (C) 506. The amino acids in which the R roups have a net positive char e at pH 7.0 are (A) (B) (C) (D) Lysine, Ar inine, Histidine Lysine, Aspar ine Histidine, Asp ar ine Glutamine, Ar inine 516. The bond in proteins that is not hydrolysed under usual conditions of denat uration: (A) Hydrophobic bond (B) Hydro en bond (C) Disulphide bond (D) Peptide bonds 517. If the amino roup and a carboxylic roup of the amino acid are attac hed to same carbon atom, the amino acid is called (A) Alpha (C) Gamma (B) Beta ( D) Delta 507. Apolipoproteins are (A) AI (C) C1 (B) AI1 (D) All of these
PROTEINS AND PROTEIN METABOLISM 518. Zymo en is (A) (B) (C) (D) An intracellular enzyme Serum enzyme A complete extracellular enzyme An inactivated enzyme (B) 1–4 units/dl (D) 50–100 units/dl (B) Hydrolase (D) Oxidase 57 528. Physiolo ically active confi uration of amino acids: (A) (B) (C) (D) L D Fo r some amino acids it is either of two Neither L nor D (B) Methionine (D) Leucin e 519. SGOT level in a adult is (A) 5–40 units/dl (C) 5–15 units/dl (A) Reductase (C) Li ase 529. Cystine is synthesized from (A) Cysteine (C) Ar inine 520. Activity of ceruloplasmin shown in vitro: 530. The major constituent of the proteins of hair and keratin of skin: (A) Ar i nine (C) Glycine (A) (B) (C) (D) (B) Cysteine (D) Ar inine 521. Increased serum alanine durin fastin is due to (A) Breakdown of muscle pr oteins (B) Decreased utilization of non essential amino acids (C) Leaka e of ami noacids to plasma (D) Impaired renal function 522. The followin 4 amino acids a re required for completion of urea cycle except (A) Aspartic acid (C) Ornithine (B) Ar inine (D) Glycine 531. NH3 is removed from brain mainly by Creatinine formation Uric acid producti on Urea formation Glutamine formation 532. Mechanism by which NH3 is removed from the kidneys is (A) (B) (C) (D) Urea formation Uric acid formation Creatinine formation None of these (B) Cholesterol (D) Phospholipids (B) Glycoproteins (D) Lipoproteins 523. Number of amino acids present in the dietary proteins: (A) 22 (C) 20 (A) Bl ood (C) Kidney (B) 23 (D) 19 (B) Liver (D) Heart 533. Low density plasma proteins are rich in (A) Chylomicrons (C) Tri lycerides 534. Transcortins are (A) Mucoproteins (C) Metalloproteins 524. Urea synthesis takes place in 525. All followin s are keto enic aminoacids except (A) Leucine (C) Phenyl alani ne (A) Tryptophan (C) Threonine (B) Isoleucine (D) Glycine (B) Ar inine (D) Phen ylalanine 535. Proteins that carries Iron into different tissues is (A) Ceruloplasmin (C) Mucoproteins (B) Trans cortin (D) Glycoproteins 526. The amino acid containin
an indole rin :
536. Naturally occurrin amino acids have (A) L Confi uration (B) D Confi uratio n (C) DL Confi uration (D) None of these 537. Abnormal chain of aminoacids in si ckle cell anemia is (A) β chain (C) γ chain (B) β chain (D) r chain 527. Histidine is converted to histamine through the process of (A) (B) (C) (D) Transamination Decar oxylation Oxidative deamination Urea cycle
58 538. A dietary deficiency of tryptophan and nicotinate leads to (A) Beri Beri (C ) Anemia (B) Xerophthalmia (D) Pellegra MCQs IN BIOCHEMISTRY 547. The amino acid which contains an indole group is (A) Histidine (C) Cystine (B) Arginine (D) Tryptophan 539. Which one of the following is an essential amino acid? (A) Arginine (C) Phe nylalanine (B) Tyrosine (D) Proline 548. From two amino acids peptide ond formation involves removal of one molecul e of (A) Water (C) Car ondioxide (B) Ammonia (D) Car oxylic acid 540. One of the following amino acid is solely ketogenic: (A) Lysine (C) Valine (B) Alanine (D) Glutamate 549. Polymers of more than 100 amino acids are termed (A) Proteins (C) Both (A) and (B) (B) Polypeptides (D) None of these 541. Along with CO2, NH3 and ATP, the amino acid that is needed in urea cycle is (A) Alanine (C) Aspartate (B) Isoleucine (D) Glycine 550. The example of glo ulins: (A) Leucosin (C) Oryzenin (B) Tu erin (D) Legunel in 542. Isoelectric pH of an amino acid is that pH at which it has a (A) Positive c harge (C) No charge (B) Negative charge (D) None of these 551. The example of scleroproteins: (A) Glutamin (C) Salmine (B) Giladin (D) Ela stin 552. The example of phosphoprotein: (A) Mucin (C) Ovomucoid (B) Ovovitellin (D) Tendomucoid 543. Which of the following contri utes nitrogen atoms to oth purine and pyrimi dine rings? (A) Aspartate (B) Car amoyl phosphate (C) CO2 (D) Glutamine 544. Whi ch amino acid is a lipotropic factor? (A) Lysine (C) Tryptophan (B) Lecuine (D) Methionine 553. The example of metalloproteins: (A) Siderophilin (C) Elastin (B) OREES muco id (D) All of these 554. The example of chromoprotein: (A) Salmine (C) Zein (B) Catalase (D) Gliadin 555. Deamination is ______ of amino group. (B) Addition (A) Removal (C) Suppleme ntation (D) None of these 556. Proteins produce polypeptides from proteins y (A ) Oxidizing (C) Hydrolyzing (B) Reducing (D) None of these 545. Which of the following protein is rich in cysteine? (A) Elastine (C) Fi rin (B) Collagen (D) Keratin 546. Which amino acid is present at 6th position of β chain of H s instead of glut amate in H A? (A) Cysteine (C) Aspartate (B) Valine (D) Glutamate 557. Proteins react with iuret reagent which is suggestive of 2 or more (A) Hyd rogen onds (B) Peptide onds (C) Disulphide onds (D) Hydropho ic onds
PROTEINS AND PROTEIN METABOLISM 558. The disulphide ond is not roken under the usual conditions of (A) Filtrat ion (C) Oxidation (B) Reduction (D) Denaturation 568. Foetal haemoglo in contain s (A) Two α nd two γ chains (B) Two β and two γ chains (C) Both (A) and (B) (D) None of
these 59 559. Insulin is oxidized to separate the protein molecule into its constituent p olypeptide chains without affectin the other part of the molecule by the use of (A) Performic acid (C) Citric acid (B) Oxalic acid (D) Malic acid 569. When haemo lobin takes up oxy en there is a chan e in the structure due to the movin closer to ether of (A) β chains (C) γ chains (B) β chains (D) α nd γ chains 560. Each hydro en bond is quite (A) Weak (C) Both (A) and (B) (B) Stron ne of these
(D) No
570. The hydro en bonds in the secondary and tertiary structure of proteins are directly attacked by (A) Salts (C) Deter ents (B) Alkalies (D) All of these 561. A coiled structure in which peptide bonds are folded in re ular manner by ( A) Globular proteins (B) Fibrous proteins (C) Both (A) and (B) (D) None of these 562. In many proteins the hydro en bondin produces a re ular coiled arran emen t called (A) α-helix (C) Both (A) nd (B) (B) β-helix (D) None of these 571. The hydrogen onds etween peptide link ges re interfered (C) S licylic cid (B) Uric cid (D) Ox lic cid 572. The digest ility of cert in den tured proteins Decre ses (C) Norm l (B) Incre ses (D) None of these
y (A) Gu nidine
y proteolytic enzymes (A)
563. M ny glo ul r proteins re st le in solution lthough they l ck in (A) Hyd rogen onds (B) S lt onds (C) Non-pol r onds (D) Disulphide onds 564. E ch tu rn of α-helix cont ins the num er of mino cids (A) 2.8 (C) 3.4 (B) 3.2 (D) 3.6 573. The ntigenic nti ody functions of proteins y den tur tion re frequently (A) Not ch nged (C) Both (A) nd (B) (B) Ch nged (D) None of these 574. In c se of severe den tur tion of protein, there is (A) Reversi le den tur tion (B) Moder te reversi le den tur tion (C) Irreversi le den tur tion (D) None of these 575. When egg l umin is he ted till it is co gul ted, the second ry nd terti ry structures of the proteins re completely lost resulting in mixtur e of r ndomly rr nged (A) Dipeptide ch ins (B) Tripeptide ch ins (C) Polypeptid e ch ins(D) All of these 565. The dist nce tr velled per turn of α-helix in nm is (A) 0.34 (C) 0.54 (B) 0.4 4 (D) 0.64 566. α -helix is disrupted B) Arginine (D) Lysine
y cert in
mino
567. α-helix is st ilized ds (D) Non-pol r onds
y (A) Hydrogen
cids like (A) Proline (C) Histidine ( onds (B) Disulphide
onds (C) S lt
on
60 576. In glycoproteins the c r ohydr te is in the form of dis cch ride units, the num er of units re (A) 50–100 (C) 400–500 (B) 200–300 (D) 600–700 MCQs IN BIOCHEMISTRY 586. If one mino cid is fed excess, the sorption of nother is (A) (B) (C) ( D) Slightly cceler ted Moder tely cceler ted Highly cceler ted Ret rded 577. The milk protein in the stom ch of the inf nts is digested ) Chymotrypsin (B) Trypsin (D) Rennin
y (A) Pepsin (C
587. Under norm l conditions, food proteins re gener lly re dily digested upto the present (A) 67 to 73 (C) 82 to 89 (B) 74 to 81 (D) 90 to 97 578. Achyli g stric is s id to e when (B) Both pepsin nd HCl (D) All of these
sence of (A) Pepsin only (C) HCl only
588. By overhe ting the nutrition l v lue of cere l proteins is (A) Incre sed (C ) Unch nged (B) Decre sed (D) None of these 579. The pH of g stric juice ecome low in (A) Hemolytic nemi (B) Pernicious nemi (C) Both (A) nd (B) (D) None of these 580. In sm ll intestine trypsin hyd rolyzes peptide link ges cont ining (A) Arginine (C) Serine (B) Histidine (D) As p rt te 589. More th n h lf of the protein of the liver nd intestin l mucos re roken down nd resynthesised in (A) 10 d ys (C) 15 d ys (B) 12 d ys (D) 18 d ys 581. Chymotrypsin in the sm ll intestine hydrolyzes peptide link ges cont ining (A) Al nine (C) V line (B) Pheynl l nine (D) Methionine 590. The h lf-life of eks (D) 1 week
nti ody protein is
out (A) 4 weeks (C) 2 weeks (B) 3 we
582. C r oxy peptid se B in the sm ll intestine hydrolyzes peptides cont ining ( A) Leucine (C) Arginine (B) Isoleucine (D) Cysteine 591. Protein n D) Epinephrine
olism is stimul ted
y (A) ACTH (C) Gluc gon (B) Testosterone (
583. The tr nsport of mino cids regul ted ers: (A) 1 (C) 3 (B) 2 (D) 4
y
ctive processes of different num
592. The met olism of protein is integr ted with th t of c r ohydr te hrough (A) Ox lo cet te (C) Isocitr te (B) Citr te (D) M l te
nd f t t
584. The third ctive process for mino cids tr nsport involves (A) (B) (C) (D) Acidic mino cids B sic mino cids Neutr l mino cids Sulphur cont ining mi no cids 593. The uilding up nd re king down of protopl sm re concerned with the met olism of (A) C r ohydr te (C) Protein (B) Lipid (D) Miner ls 585. The neutr l NADP+
mino
cids for
sorption need (A) TPP (C) NAD+ (B) B6 – PO4 (D)
594. The mino cids str cted from the liver re not utilized for rep ir or sp eci l synthesis ut re roken down to (A) Keto cids (C) W ter (B) Sulphur diox ide (D) Ammoni
PROTEINS AND PROTEIN METABOLISM 595. The unw nted mino cids str cted from the tissues re either used up y the tissue or in the liver converted into (A) Ammoni (C) Ammonium s lts (B) Ure (D) Uric cid 61 603. The tr ns min se ctivity needs the coenzyme: (A) ATP (C) FAD+ 604. Tr ns m in tion is (A) Irreversi le process(B) Reversi le process (C) Both (A) nd (B) (D) None of these 605. Most mino cids re su str tes for tr ns min tion excep t (A) Al nine (C) Serine (B) Threonine (D) V line (B) B6 – PO4 (D) NAD+ 596. Amino cids provide the nitrogen for the synthesis of (A) (B) (C) (D) The ses of the phospholipids Uric cid Glycolipids Chondroitin sulph tes 597. The met olism of ll proteins ingested over nd ove the essenti l requir ements is c lled (A) (B) (C) (D) Exogenous met olism Endogenous met olism Both (A) nd (B) None of these 606 Oxid tive conversion of m ny mino cids to their corresponding -keto cids o ccurs in m mm li n: (A) Liver nd kidney (B) Adipose tissue (C) P ncre s (D) Int estine 607. The α-keto cid is dec r oxyl ted y H2O2 forming c r oxylic cid wit h one c r on tom less in the sence of the enzyme: (A) C t l se (C) De min se (B) Dec r oxyl se (D) Phosph t se 598. Sulphur cont ining mino cids fter c t olism produces is excreted: (A) SO2 (C) H2SO4 (B) HNO3 (D) H3 PO4
su st nce which
599. Ethere l sulph te is synthesized from the _________ (C) B sic (B) Acidic (D) Sulphur cont ining
cid. (A) Neutr l
mino
608. The ctivity of m mm li n L- mino cid oxid se, n FMN – fl vo protein, is qu ite (A) Slow (C) Both (A) nd (B) (B) R pid (D) None of these 600. The mino cids required for cre tine form tion: (A) Glycine (C) Methionine (B) Arginine (D) All of these 609. From diet ry protein s well s from the ure present in fluids secreted in to the g strointestin l tr ct intestin l cteri produce (A) (B) (C) (D) C r on dioxide Ammoni Ammonium sulph te Cre tine 601. In hum n nd other ureotelic org nisms, the end product of mino cid nitro gen met olism: (A) Bile cids (C) Ure (B) Ketone odies (D) B rium sulph te 610. The symptom of mmoni intoxic tion includes (A) Blurring of vision (B) Con stip tion (C) Ment l confusion (D) Di rrhoe 611. Ammoni intoxic tion symptoms occur when r in mmoni levels re (A) Slightly diminished (B) Highly diminishe d (C) Incre sed (D) All of these 602. The end product of mino cid nitrogen met olism in uricotelic org nisms ( reptiles nd irds) is (A) Biliru in (C) Uric cid (B) Ure (D) Biliverdin
62 612. Ammoni production y the kidney is depressed in (A) Acidosis (C) Both (A) nd (B) (B) Alk losis (D) None of these MCQs IN BIOCHEMISTRY 621. In severe cidosis, the output of ure is (A) Decre sed (B) Slightly incre sed (C) Highly incre sed (D) Moder tely incre sed 622. Uremi occurs in (A) Cirr hosis of the liver(B) Nephritis (C) Di etes mellitus (D) Coron ry throm osis 62 3. Clinic l symptom in ure cycle disorder is (A) Ment l ret rd tion (B) Drowsin ess (C) Di rrhoe (D) Oedem 624. The sp ring ction of methionine is (A) Tyrosi ne (C) Arginine (B) Cystine (D) Tryptoph n 613. Ammoni is excreted s mmonium s lts during met olic cidosis ut the m j ority is excreted s (A) Phosph tes (C) Uric cid (B) Cre tine (D) Ure 614. Synthesis of glut mine is (B) ADP (D) Cre tin phosph te
ccomp nied
y the hydrolysis of (A) ATP (C) TPP
615. In r in, the m jor met olism for remov l of mmoni A) Glut m te (C) Asp r gine (B) Asp rt te (D) Glut mine
is the form tion of (
625. NH + 4 min tes glut m te to form glut mine requiring ATP nd (A) K + (C) C ++ 626. Glut thione is (A) Dipeptide (C) Polypeptide (B) Tripeptide (D) None of these (B) N + (D) Mg ++ 616. C r moyl phosph te synthet se structure is m rked y ch nge in the presenc e of (A) (B) (C) (D) N-Acetyl glut m te N-Acetyl Asp rt te Neur minic cid Ox l te 627. All following re conjug ted proteins except (A) Nucleoproteins (C) Met llo proteins (B) Proteoses (D) Fl voproteins 617. The iosynthesis of Ure occurs m inly in the Liver: (A) (B) (C) (D) Cytoso l Microsomes Nucleus Mitochondri 628. All α- mino cids h ve one symmetric c r on rtic cid (B) Glycine (D) Histidine 618. One mol. of Ure is synthesized (A) 2 (C) 4 (B) 3 (D) 5
tom except (A) Arginine (C) Asp
t the expense of the _______ mols. of ATP.
629. Num er of mino cids present in pl nts, ) 20 (C) 150 (A) (B) (C) (D) (B) 80 (D) 200
nim ls
nd micro i l proteins: (A
630. Hydr ted density of (HD) lipoproteins is 0.94 gm/ml 0.94-1.006 gm/ml 1.0061.063 gm/ml 1.063-1.21 gm/l 619. Ure iosynthesis occurs m inly in the liver involving the num er of cids: (A) 3 (C) 5 (B) 4 (D) 6 620. The norm l d ily output of Ure to 30 (B) 15 to 25 (D) 25 to 35
mino
through urine in gr ms: (A) 10 to 20 (C) 20
631. The ond in proteins th t is not roken under usu l conditions of den tur t ion: (A) Hydropho ic ond (B) Hydrogen ond (C) Disulphide ond (D) Peptide ond s
PROTEINS AND PROTEIN METABOLISM 632. Pl sm proteins ct s (A) Buffers (C) Reserve proteins (B) Immunoglo ulins (D) All of these 63 642. A norm l ch in of mino cids in sickle cell Delt ch in (B) Bet ch in (D) G m ch in
n emi
is (A) Alph
ch in (C)
633. Group th t re cts in the Biuret test: (A) Peptide (B) Amino group (C) C r o xylic group (D) Aldehyde group 634. In nitroprusside test, mino cid cysteine p roduces : (A) Red colour (C) Yellow colour (B) Blue colour (D) Purple colour 643. Num er of ch ins in glo in p rt of norm l H : (A) 1 (C) 3 (A) 3.6 (C) 5.0 ( B) 2 (D) 4 (B) 4.7 (D) 6.1 644. The PH of
l umin is
635. Protein present in hemoglo in h s the structure known rti ry (B) Second ry (D) Qu rtern ry
s (A) Prim ry (C) Te
645. Ninhydrin re ction gives purple colour nd evolves CO2 with (A) Peptide onds (C) Ergothioneine (B) Hist mine (D) Asp rgine 636. Isoelectric pH of n mino cid is th t pH t which it h s h rge (C) Nil net ch rge (B) Neg tive ch rge (D) None of these
(A) Positive c
646. Den tur tion of proteins involves re kdown of (A) Second ry structure(B) T erti ry structure (C) Qu rtern ry structure(D) All of these 647. In den tur tion of proteins, the ond which is not roken: (A) Disulphide ond (B) Peptide ond (C) Hydrogen ond (D) Ionic ond 648. The purity of n isol ted protein c n e tested y employing v rious methods. (A) (B) (C) (D) (E) Solu ility curve Molecu l r weight Ultr Centrifug tion Immuno R ctivity All of these 637. Al uminoids ) None of these
re simil r to (A) Al umin (C) Both (A)
nd (B) (B) Glo ulin (D
638. Optic l isomers of ll mino cids exist except (A) Glycine (C) Al nine (B) Arginine (D) Hydroxy proline 639. Proteins th t constitute ker tin, coll gen nd el stin in ody mines (C) Scleroproteins (B) Phosphol proteins (D) Met proteins 640. System tic n me of lysine is (A) (B) (C) (D) Amino ex noic cid Aminosuccinic cid 2-Aminoprop noic cid
cetic
re (A) Prot
cid 2,6 di minoh
649. More th n one re k in the line or in s tur tion curve indic tes the follow ing qu lity of protein. (A) Non homogenity (B) Purity (C) Homogeneity (D) None o f these 650. A sh rp moving ound ry is o t ined etween the pure solvent nd so lute cont ining l yer in (A) (B) (C) (D) Chrom togr phy Immuno Re ctivity Ultr Centrifug tion Solu ility curve 641. Side ch ins of ll following mino cids cont in rom tic rings except (A) Phenyl l nine (C) Tyrosine (B) Al nine (D) Tryptoph n
64 651. The nti odies r ised g inst pure protein will show only one sh rp spike on this technique: (A) (B) (C) (D) Solu ility curve Solvent precipit tion Molec ul r weight determin tion Immuno electrophoresis MCQs IN BIOCHEMISTRY 658. The sorting out of molecules ccording to size nd sh pe m y e d pted to protein purific tion in this technique: (A) (B) (C) (D) Adsorption chrom togr ph y Gel filtr tion chrom togr phy P per chrom togr phy None of these 652. This technique t kes the dv nt ge of the f ct th t e ch protein h s differ ent pH t which it is electric lly neutr l i.e., its isoelectric pH: (A) (B) (C) (D) Isoelectric focussing Immunoel Ectro Phoresis Chrom togr phy HPLC 659. Frequently employed m teri ls for the dsorption chrom togr phy of proteins include (A) High c p city supporting gel (B) St rch locks (C) C lcium phosph t e gel lumin gel nd hydroxy p tite (D) All of these 660. The solu ility of mo st proteins is lowered t high s lt concentr tions is c lled s (A) S lting in p rocess (B) S lting out process (C) Isoelectric focussing(D) None of these 661. P henyl l nine, ornithine nd methionine re involved in the iogenesis of (A) Lys ergic cid (C) L-Hyoscy mine (B) Reserpine (D) P p verine 653. The following technique m kes use of the difference in net ch rges of prote ins t given pH: (A) (B) (C) (D) Thin l yer chrom togr phy Ion exch nge chrom togr phy High perform nce liquid chrom togr phy P per chrom togr phy 654. The r tio of the dist nce moved y compound to the dist nce moved y the solvent frent is known s its (A) PI v lue (C) Rf v lue (B) Linking num er (D) G old num er 662. All the following diuretics inhi it the c r onic nhydr se except (A) Acet zol mide (C) Furosemide (A) Sug rs (C) Amino cids (B) Bumet nide (D) Eth crynic cid (B) Phenols (D) C r oxylic cids 655. The movement of ch rged p rticles tow rds one of the electrodes under the i nfluence of electric l current is (A) (B) (C) (D) Gel filtr tion Molecul r sievi ng G s liquid chrom togr phy Electrophoresis 663. Protein is
polymer of
664. All the following mino cids re optic lly C) V line (B) Phenyl l nine (D) Glycine
ctive except (A) Tryptoph ne (
656. An nion exch nge resin linked to cellulose (C) Seph dex (B) CM cellulose (D) None of these
ck one is (A) DEAE cellulose
665. Proteinous su st nces which c t lyze iochemic l re ctions re known s (A) Activ tors (C) Enzymes (A) Blood clotting (C) Digestion (B) C t lysts (D) Hormo nes (B) Met olic p thw y (D) Kre ’s cycle 657. A c tion exch nge resin linked to cellulose ) St rch (B) DEAE cellulose (D) Biogel 666. Insulin is
protein which controls
ck one is (A) CM-cellulose (C
PROTEINS AND PROTEIN METABOLISM 667. Proteins which re responsi le for defence mech nism olites (C) Antimycins (B) Anti odies (D) Apoproteins
re c lled (A) Antimet
65 677. Protein deficiency dise se is known s (A) (B) (C) (D) (A) (B) (C) (D) Cush ing’s dise se F ry’s dise se P rkinson’s dise se Kw shiorkor nd m r smus Egg pl nt S oy e n Tree of the He ven Devil’s dung 668. When the net ch rge on n mino cid is zero, the pH is m int ined s? (A) 4.5 (C) 7.0 (A) Cryst llis tion (C) Solu ility (B) 11.2 (D) 9.1 (B) Precipit tio n (D) Re ctivity 678. A veget
le source of protein is
669. Isoelectric point of
mino
cids is used for
670. X nthoproteic test is positive in proteins cont ining (A) (B) (C) (D) Sulph ur mino cids α-Amino cids Arom tic mino cids Aliph tic mino cids (B) Biuret e test (D) Ninhydrine test 679. Ox lo cet te is converted to sp rtic (A) (B) (C) (D) (B) Oxid se (D) C t l se 680. Deficiency of hesis Kidney F tty 671. All α - mino
cid
y (A) Reduct se (C) Tr nsmin se
iotin results in decre se in Amino cid synthesis
cid synthesis Lipid synt
cids give positive (A) Million’s test (C) X nthproteic test
672. N-termin l mino cids of polypeptide re estim ted y (A) Edm nn re ctio n (B) S nger’s re gent (C) Form ldehyde test (D) Ninhydrine re ction 673. Million’s test is positive for (A) Phenyl l nine (C) Tyrosine (B) Glycine (D) Proline 681. The precursor of ile s lts, sex hormones nd vit min D is (A) Diosgenin (C ) C mpesterol (A) (B) (C) (D) (A) (B) (C) (D) (B) Cholesterol (D) Ergosterol 682 Uns tur ted f tty cids is known s Non-essenti l f tty cids Essenti l f tt y cids Cere rosides Phospholipids Two peptide link ge Phenolic group Imid zole ring None of these 674. Indole group of tryptoph n responses positively to (A) Glyoxylic iuret test (B) Schiff’s re gent (D) Resorcinol test
cid (C) B
683 Biuret test is specific for 675. Gu nidine group of rgentine gives positive test with (A) (B) (C) (D) Le d cet te S k guchi re gent Tricholoro cetic cid Molisch’s re gent 684. Most of c lood is c lled (D) Colloid l s lt form is c
lcium is present in one, ut 2% present in soft tissue nd the (A) C lcin ted lood (B) Solidified lood (C) Physiologic l lood lood 685. C lcium present with protein is known s free while in lled s (A) Bound (C) Solid (B) Precipit ted (D) Polymorphs
676. Thiol group of cysteine gives red colour with (A) (B) (C) (D) Sodium e Le d cet te Sodium nitroprusside B rfoed’s re gent
cet t
66 686. The following ions help in enzym tic tr nsfer of phosph te from ATP to pyru vic cid: (A) Sodium (C) M gnesium (B) C lcium (D) Pot ssium MCQs IN BIOCHEMISTRY 695. Pl telets cont in n enzyme which h s import nt role in clotting in lood. This enzyme is known s (A) Cholinester se (C) Dec r oxyl se (B) Tr ns min se (D ) Throm okin se 687. Intern tion l enzyme commission cl ssifies enzymes into (A) Three cl sses ( C) Four cl ssess (B) Six cl sses (D) Ten cl sses 696. Tre tment of pentoses with concentr ted miner l cid yields cyclic hyde known s (A) Pent ldehyde (C) Hex ldehyde (B) Cyclopent l (D) Furfur l
lde
688. Mich elis – Menten equ tion is used to expl in the effect of su str te concen tr tion on (A) C r ohydr te (C) Lipid (B) Enzyme (D) Protein 697. Isoelectric pH is th t pH t which protein is electric lly: (A) Neutr l (C) C tionic (B) Anionic (D) None of these 689. The pH t which n enzyme h s m ximum pH (C) Low pH (B) Optimum pH (D) High pH
ctivity is known
s (A) Isoelectric
698. A out 6.25 g of h emoglo in is produced nd destroyed in the ody e ch d y nd the tot l mount of h emoglo in in norm l he lthy 70 kg weighing m le dul t is (A) 250 g (C) 100 g (B) 150 g (D) 70 g 690. Degr d tion of proteins to mino cids, glucose from c r ohydr tes nd f tt y cids from lipids is known s (A) An olism (C) C t olism (B) Met olism (D) Cretinism 699. P ncre tic juice cont ins ll of the following except (A) Trypsinogen (C) C holecystokinin (B) Lip se (D) Chymnotrypsinogen 691. During glycolysis of glucose the energy li er ted in the sence of oxygen is known s (A) (B) (C) (D) Oxygenesis Glyconeogenesis Glycogenolysis An ero ic ferment tion 700. The milk protein in the stom ch in Cl (B) Rennin (D) Chymotrypsinogen
n
dult is digested
y (A) Pepsin (C) H
692. Deficiency of ure cycle enzymes results into ccumul tion of citrulline r gininosuccin te rginine in the liver resulting in incre sing concentr tion of …….. in the lood. (A) C lcium (C) Ammoni (B) Sodium (D) Lipid 701. C r oxypeptid se, n enzyme of p ncre tic juice, cont ins (A) Mn (C) M gnes ium (B) Zinc (D) M ng nese 693. Accumul tion of trytoph n in lood is known s (A) Pompe’s dise se (B) Wilson’s dise se (C) Wolm n’s dise se (D) H rtnup’s dise se 694. Lymphocytes re responsi le for the form tion of (A) Serum (C) Anti ody (B) Pl sm (D) C lcium 702. The zymogen from trypsinogen of p ncre tic juice is converted to psin y (A) Peisin (C) Enterokin se (B) Enterocrinin (D) Rennin
ctive try
703. In ctive zymogens re precursors of ll the following g strointestin l enzy mes except (A) C r oxypeptid se (B) Pepsin (C) Amino peptid se (D) Chymotrypsin
PROTEINS AND PROTEIN METABOLISM 704. Rennin cts on c sein of milk in inf nts in presence of (A) Mg ++ (C) Co++ (B) Zn ++ (D) C ++ 67 713. The milk protein in the stom ch of the inf nts is digested ) Chymotrypsin (B) Trypsin (D) Rennin
y (A) Pepsin (C
705. All the following re true out phenylketonuri except (A) Deficiency of p henyl l nine hydroxyl se (B) Ment l ret rd tion (C) Incre sed urin ry excretion of p-hydroxyphenyl pyruvic cid (D) Decre se serotonin form tion 706. Which of t he mino cid produces v sodil tor on dec r oxyl tion? (A) Glut min cid (C) O rnithine (B) Histidine (D) Cysteine 714. Protein n D) Epinephrine
olism is stimul ted
715. The num er of helices present in D) 4
y (A) ACTH (C) Gluc gon (B) Testosterone ( coll gen molecule is (A) 1 (C) 3 (B) 2 (
716. Which ond is present in the prim ry structure of protein? (A) Ester (C) Io nic ond (B) Hydrogen (D) Peptide 707. Neutr l e
mino
cid is (A) Leucine (C) Asp rtic
cid (B) Lysine (D) Histidin
717. S k guchi re ction is specific for (A) Gu nidine group (B) Phenolic group ( C) C r oxylic group (D) None of these 718. With the exception of glycine ll mi no cids found in protein re (A) Isocitr te dehydrogen se (B) Fum r se (C) Succ in te thiokin se (D) ATP se 719 In protein structure the α -helix nd β ple ted shee ts re ex mple of (A) Prim ry structure (C) Terti ry structure (B) Second ry str ucture (D) Qu tern ry structure 708. The mino cid cont ining hydroxy group: (A) Glycine (C) Arginine (B) Isole ucine (D) Thereonine 709. The mino cid which synthesizes m ny hormornes: (A) V line (C) Al nine (B) Phenyl l nine (D) Histidine 710. Insulin degr d tion of disulfide ond form tion is effected y (A) (B) (C) (D) Pyruv te dehydrogen se Xylitol reduct se Gut thione reduct se X nthine oxid se 720. An essenti l (D) Tyrosine
mino
cid in m n is (A) Proline (C) Asp r gine (B) Threonine
711. A protein re cts with iuret re gent which indic tes 2 or more (A) Blood cl otting (B) Peptide ond (C) Disulphide onds (D) Hydropho ic onds 712. In m ny proteins the hydrogen onding produces regul r coiled rr ngement which is c l led s (A) β-Helix (C) Both (A) nd (B) (B) α-Helix (D) Spir l 721. An mino cid th t does not form (B) Tyrosine (D) Proline
n α helix is (A) Asp r gine (C) Tryptoph n
722. The protein present in h ir is (A) El stin (C) Ker tin (B) Prol mine (D) Gl i din
68 723. Pl sm protein c n e sep r ted y (A) (B) (C) (D) (A) (B) (C) (D) S lting out with (NH4)2SO4 Ultr centrifug tion Immuno electrophoresis All of these Ur ci l Adenine Hydroxy methyl cytosine Phosph te (A) TPP (C) Biotin MCQs IN BIOCHEMISTRY (B) Mg ++ (D) CoA-SH 727. A polymeric unit of st rch which h s r nched structure is (A) Glucose (C ) Isom ltose (A) (B) (C) (D) (B) Amylopectin (D) Amylose 724. RNA does not cont in 728 The repe ting unit in hy luronic cid is Glucuronic cid nd G l ctos mine G lucuronic cid re glucos mine Glucuronic cid nd N- cetyl glucos mine Glucuron ic cid nd N- cetyl g l ctos mine 725. In m mm li n cells, ri osom l RNA is produced m inly in the (A) (B) (C) (D) Nucleus Nucleolus Ri osome Golgi pp r tus 729 The repe ting dis cch ride unit in celluslose is (A) Sucrose (C) Dextrose (B ) M ltose (D) Cello iose 726. Which co-enzyme is not involved in oxid tive dec r oxyl tion of pyruvic d?
ci
PROTEINS AND PROTEIN METABOLISM 69 ANSWERS 1. A 7. A 13. A 19. B 25. A 31. C 37. B 43. B 49. D 55. B 61. B 67. A 73. A 79. A 85. C 91. A 97. A 103. D 109. D 115. D 121. B 127. B 133. A 139. A 145. A 151. A 157. D 163. A 169. C 175. D 181. C 187. D 193. B 199. B 205. D 211. C 217. B 223. C 229. C 235. C 241. B 247. C 2. A 8. A 14. C 20. C 26. C 32. B 38. C 44. C 50. A 56. A 62. A 68. D 74. B 80. A 86. B 92. B 98. D 104. D 110. A 116. C 122. B 128. C 134. A 140. D 146. B 152. B 158. D 164. D 170. C 176. C 182. C 188. A 194. C 200. B 206. C 212. A 218. D 224. C 230. A 236. B 242. B 248. D 3. A 9. A 15. C 21. B 27. B 33. D 39. C 45. C 51. A 57. C 63. D 69. A 75. A 81. C 87. B 93 . C 99. A 105. A 111. A 117. A 123. A 129. A 135. A 141. C 147. B 153. C 159. C 165. C 171. D 177. B 183. B 189. B 195. D 201. C 207. D 213. C 219. B 225. C 231 . C 237. A 243. C 249. B 4. A 10. D 16. B 22. A 28. B 34. B 40. B 46. A 52. A 58 . B 64. C 70. A 76. A 82. A 88. A 94. D 100. A 106. A 112. A 118. A 124. A 130. A 136. A 142. A 148. B 154. C 160. C 166. B 172. B 178. B 184. C 190. D 196. B 2 02. D 208. B 214. A 220. B 226. D 232. D 238. D 244. A 250. C 5. A 11. B 17. B 2 3. B 29. A 35. C 41. B 47. A 53. D 59. C 65. D 71. C 77. D 83. C 89. A 95. A 101 . D 107. A 113. A 119. D 125. A 131. B 137. C 143. C 149. D 155. B 161. B 167. D 173. A 179. A 185. B 191. C 197. D 203. C 209. A 215. C 221. C 227. C 233. D 23 9. B 245. B 251. C 6. A 12. A 18. C 24. D 30. A 36. A 42. A 48. B 54. A 60. A 66 . C 72. B 78. D 84. D 90. A 96. A 102. D 108. C 114. B 120. C 126. A 132. C 138. A 144. B 150. A 156. C 162. D 168. D 174. D 180. A 186. C 192. C 198. C 204. C 210. D 216. D 222. D 228. D 234. D 240. D 246. A 252. A
70 253. D 259. D 265. A 271. C 277. B 283. B 289. D 295. B 301. A 307. A 313. D 319 . D 325. B 331. C 337. C 343. A 349. B 355. D 361. B 367. A 373. B 379. D 385. C 391. D 397. D 403. B 409. B 415. D 421. D 427. D 433. B 439. C 445. C 451. C 45 7. D 463. A 469. A 475. B 481. C 487. C 493. B 499. D 505. B 254. D 260. D 266. B 272. B 278. D 284. C 290. B 296. C 302. B 308. A 314. C 320. B 326. B 332. D 3 38. A 344. B 350. B 356. D 362. B 368. C 374. D 380. B 386. A 392. D 398. B 404. C 410. D 416. C 422. A 428. D 434. A 440. D 446. B 452. D 458. C 464. C 470. D 476. D 482. B 488. D 494. A 500. D 506. A 255. D 261. D 267. B 273. C 279. C 285 . A 291. D 297. B 303. B 309. C 315. B 321. A 327. A 333. C 339. A 345. C 351. C 357. C 363. D 369. A 375. A 381. D 387. A 393. D 399. B 405. D 411. B 417. B 42 3. C 429. A 435. A 441. C 447. D 453. A 459. A 465. C 471. C 477. C 483. C 489. B 495. B 501. C 507. D 256. B 262. D 268. D 274. C 280. D 286. D 292. C 298. C 3 04. C 310. D 316. D 322. B 328. C 334. B 340. C 346. B 352. C 358. B 364. B 370. A 376. B 382. D 388. B 394. D 400. A 406. D 412. B 418. C 424. D 430. B 436. A 442. C 448. C 454. A 460. C 466. D 472. B 478. C 484. B 490. B 496. B 502. C 508 . B MCQs IN BIOCHEMISTRY 257. D 263. D 269. B 275. D 281. A 287. B 293. D 299. B 305. B 311. B 317. B 323 . D 329. B 335. C 341. D 347. B 353. B 359. D 365. D 371. D 377. A 383. D 389. C 395. C 401. B 407. B 413. C 419. A 425. D 431. D 437. B 443. B 449. C 455. B 46 1. B 467. B 473. A 479. B 485. B 491. C 497. A 503. C 509. A 258. B 264. B 270. D 276. B 282. D 288. B 294. D 300. C 306. B 312. D 318. B 324. A 330. D 336. B 3 42. B 348.B 354. C 360. D 366. D 372. B 378. B 384. D 390. D 396. B 402. A 408. B 414. C 420. D 426. C 432. A 438. B 444. D 450. C 456. C 462. A 468. A 474. B 4 80. D 486. C 492. C 498. C 504. C 510. C
PROTEINS AND PROTEIN METABOLISM 511. B 517. A 523. C 529. A 535. B 541. C 547. D 553. A 559. A 565. C 571. A 577 . D 583. C 589. A 595. B 601. C 607. A 613. D 619. D 625. D 631. D 637. A 643. D 649. A 655. D 661. A 667. B 673. C 679. C 685. A 691. D 697. A 703. C 709. B 71 5. C 721. D 727. B 512. D 518. D 524. B 530. B 536. A 542. C 548. A 554. B 560. A 566. A 572. A 578. B 584. C 590. C 596. A 602. C 608. A 614. A 620. C 626. B 6 32. D 638. A 644. B 650.C 656. A 662. D 668. C 674. A 680. D 686. D 692. C 698. D 704. D 710. C 716. D 722. C 728. C 513. D 519. A 525. D 531. D 537. B 543. A 5 49. A 555. A 561. A 567. A 573. B 579. B 585. B 591. B 597. A 603. B 609. B 615. D 621. A 627. B 633. A 639. C 645. D 651. D 657. A 663. C 669. B 675. B 681. B 687. B 693. D 699. C 705. C 711. B 717. A 723. D 729. D 514. A 520. D 526. A 532 . D 538. D 544. D 550. B 556. C 562. A 568. A 574. C 580. A 586. D 592. A 598. C 604. B 610. A 616. A 622. B 628. B 634. A 640. B 646. D 652. A 658. B 664. D 67 0. C 676. C 682. B 688. B 694. C 700. A 706. B 712. B 718. B 724. C 515. A 521. A 527. B 533. B 539. C 545. D 551. D 557. B 563. D 569. A 575. C 581. B 587. D 5 93. C 599. D 605. B 611. C 617. D 623. A 629. D 635. D 641. B 647. B 653. B 659. C 665. C 671. D 677. D 683. A 689. B 695. D 701. B 707. A 713. D 719. B 725. B 516. C 522. D 528. A 534. A 540. A 546. B 552. B 558. D 564. D 570. B 576. D 582 . C 588. B 594. D 600. D 606. A 612. B 618. B 624. B 630. B 636. C 642. B 648. C 654. C 660. B 666. B 672. A 678. B 684. C 690. C 696. D 702. C 708. D 714. B 72 0. B 726. C 71
72 MCQs IN BIOCHEMISTRY EXPLANATIONS FOR THE ANSWERS 12. A Al umin (mol. Wt. 69,000) is the m jor constituent of pl sm proteins with concentr tion 3.5–5.0 g/dl. It is exclusively synthesized y the liver. Pl sm l umin performs osmotic, tr nsport nd nutritive function, esides the ufferin g ction. Cerulopl smin is lue coloured, copper cont ining α 2–glo ulin. Its norm l pl sm concentr tion is round 30 mg/dl nd it is decre sed in Wilson’s dise se . Defects in clotting f ctors c use norm lities in lood clotting. Hemophili A (defect ntihemophilic f ctor i.e., VII), hemophili B or Christm s dise se (de fect-Christm s f ctor, i.e., IX) re the m jor norm lities known. Lysine, rgi nine, histidine. These re di sic monoc r oxylic cids. The mino cids which re never found in protein structure re collectively referred to s non-protein mino cids. However, the nonprotein mino cids perform sever l iologic l func tions. e.g., ornithine, citrulline, thyroxine. Amino cids re divided into 3 gr oups sed on their met olic f ts. 507. D 488. D or n incre se in glo ulins (c hronic infections). 421. D By s lting out technique (using mmonium sulf te or s odium sulf te), the pl sm proteins c n e sep r ted into 3 groups – l umin, glo ulins nd fi rinogen. Electrophoresis is the most commonly employed n lytic l t echnique for the sep r tion of pl sm (serum) proteins. P per or g r gel electr ophoresis with veron l uffer (pH 8.6) sep r tes pl sm proteins into 5 distinct nds n mely l umin, α1 - α2, β- nd γ lobulins. Complement system is composed of abou t 20 plasma proteins that complement the functions of antibodies in defendin th e body from invadin anti ens. The complement system helps the body immunity by promotin pha ocytosis, formation of anti en antibody complexes and inflammatory reaction. Apolipoproteins or apoproteins are the (structural) protein component s of lipoproteins and are closely involved in the metabolism of the later, e. ., AI, AIII, B100, C1, CII The removal of amino roup from the amino acids as ammo nia is deamination. It may be oxidative or non oxidative in nature. The NH3 so l iberated is used for synthesis or urea. The three amino acids lycine, ar inine and methionine are required for creatine formation. Glycine combines Biuret test is answered by compounds containin two or more CO– NH roups i.e., peptide bonds . All protein and peptides possessin at least two peptide linka es i.e., tripep tide (with 3 amino acids) ive positive biuret test. The principle of biuret tes t is conveniently used to detect the presence of proteins in biolo ical fluids. The mechanism of biuret test is not clearly known. It is believed that the colou r is due to the formation of a copper co ordianated complex. Ar inine, containin uanidine roup, reacts with α-n phthol nd lk line hypo romite to form red c olour complex. 67. A 103. D 151. A 212. A 268. D 555. A ( ) Glycogenic: These mino cids c n serve s precursors for the synthesis of gluco se (or glycogen) e.g., l nine, sp rt te, glycine. Ketogenic: F t c n e synthe sized from these mino cids e.g., leucine, lysine. Glycogenic or ketogenic: The mino cids th t c n form glucose s well s f t e.g., isoleucine, phenyl l nin e, lysine. Zwitterion (dipol r ion) is hy rid molecule cont ining positive nd neg tive i
onic groups. E ch mino cid h s ch r cteristic pH (e.g., leucine pH 6.0), t which it exists s zwitterions. Al umin/Glo ulin (A/G) r tio expresses their rel tion in the serum concentr tion. The norm l A/G r tio is 1.2 to 1.5:1, t king t he concentr tion of l umin nd glo ulins respectively in the r nge of 3.5-5.0 g /dl nd 2.5–3.5 g/dl. The A/G r tio is lowered either due to decre se in l umin 9liver dise se) 600. D ( ) (c) 683. A 300. C 350. B 717. A
FATS AND FATTY ACID METABOLISM 73 CHAPTER 4 ETABOLISM FATS AND FATTY ACID METABOLISM 1. An ex mple of hydroxy f tty ) Crotonic cid (D) Oleic cid
cid is (A) Ricinoleic
8. In hum ns, diet ry essenti l f tty (B) Ste ric cid (D) Linoleic cid 2. An ex mple of s tur ted f tty B) Oleic cid (D) Erucic cid
cid (C) Butyric
cid is (A) P lmitic
cid is (A) P lmitic
cid (B
cid (C) Oleic
cid (C) Linoleic
cid
cid (
9. A lipid cont ining lcoholic mine residue is (A) Phosph tidic cid (B) G ngl ioside (C) Glucocere roside (D) Sphingomyelin 10. Ceph lin consists of (A) Glyce rol, f tty cids, phosphoric cid nd choline (B) Glycerol, f tty cids, phospho ric cid nd eth nol mine (C) Glycerol, f tty cids, phosphoric cid nd inosito l (D) Glycerol, f tty cids, phosphoric cid nd serine 11. In m mm ls, the m jo r f t in dipose tissues is (A) Phospholipid (C) Sphingolipids (B) Cholesterol ( D) Tri cylglycerol 3. If the f tty cid is esterified with n lcohol of high molecul r weight inst e d of glycerol, the resulting compound is (A) Lipositol (C) W x (B) Pl sm logen (D) Ceph lin 4. A f tty cid which is not synthesized in the ody nd h s to e supplied in t he diet is (A) P lmitic cid (C) Linolenic cid 5. Essenti l f tty cid: (A) Lin oleic cid (B) Linolenic cid (C) Ar chidonic cid (D) All these 6. The f tty c id present in cere rosides is (A) Lignoceric cid (C) C prylic cid (B) V leric cid (D) Behenic cid (B) L uric cid (D) P lmitoleic cid 12. Glycosphingolipids re com in tion of (A) Cer mide with one or more sug r residues (B) Glycerol with g l ctose (C) Sphingosine with g l ctose (D) Sphingos ine with phosphoric cid 7. The num er of dou le
onds in
r chidonic
cid is (A) 1 (C) 4 (B) 2 (D) 6
74 13. The import nce of phospholipids s constituent of cell mem r ne is ec use t hey possess (A) (B) (C) (D) F tty cids Both pol r nd nonpol r groups Glycerol Phosphoric cid MCQs IN BIOCHEMISTRY 22. G ngliosides derived from glucosylcer mide cont in in ddition one or more m olecules of (A) Si lic cid (C) Di cylglycerol (B) Glycerol (D) Hy luronic cid 14. In neutr l f ts, the uns ponific le m tter includes (A) Hydroc r ons (C) Ph ospholipids (B) Tri cylglycerol (D) Cholsesterol 23. ’Drying oil’, oxidized spont neously y tmospheric oxygen t ordin ry temper tu re nd forms h rd w ter proof m teri l is (A) Coconut oil (C) R pe seed oil (B ) Pe nut oil (D) Linseed oil 15. Higher lcohol present in w xes is (A) Benzyl (C) Ethyl 16. Ker sin consists of (A) Nervonic cid (C) Cervonic cid (B) Lignoceric cid (D) Clup nodonic ci d (B) Methyl (D) Cetyl 24. Deterior tion of food (r ncidity) is due to presence of (A) (B) (C) (D) Chol esterol Vit min E Peroxid tion of lipids Phenolic compounds 17. G ngliosides re complex glycosphingolipids found in (A) Liver (C) Kidney (B ) Br in (D) Muscle 25. The num er of ml of N/10 KOH required to neutr lize the f tty cids in the d istill te from 5 gm of f t is c lled (A) (B) (C) (D) Reichert-Meissel num er Pol enske num er Acetyl num er Non vol tile f tty cid num er 18. Uns tur ted f tty cid found in the cod liver oil nd cont ining 5 dou le o nds is (A) (B) (C) (D) Clup nodonic cid Cervonic cid El idic cid Timnodonic cid 26. Molecul r formul of cholesterol is (A) (B) (C) (D) C27H45OH C29H47OH C29H47 OH C23H41OH Benzene deriv tive Quinoline deriv tive Steroid Str ight ch in cid 19. Phospholipid cting s surf ct nt is (A) Ceph lin (C) Lecithin (B) Phosph ti dyl inositol (D) Phosph tidyl serine 27. The cholesterol molecule is (A) (B) (C) (D) 20. An oil which cont ins cyclic f tty cids nd once used in the tre tment of l eprosy is (A) El idic oil (C) L noline (B) R peseed oil (D) Ch ulmoogric oil 28. S lkowski test is performed to detect (A) Glycerol (C) F tty sterol (D) Vit min D
cids (B) Chole
21. Unple s nt odours nd t ste in f t (r ncidity) c n e del yed or prevented y the ddition of (A) Le d (C) Tocopherol (B) Copper (D) Ergosterol 29. P lmitic, oleic or ste ric cid ester of cholesterol used in m nuf cture of cosmetic cre ms is (A) El idic oil (C) Sperm ceti (B) L noline (D) Ch ulmoogric oil
FATS AND FATTY ACID METABOLISM 30. Diet ry f ts fter sorption B) VLDL (D) Chylomicron
ppe r in the circul tion
s (A) HDL (C) LDL (
75 38. An import nt finding of F ry’s dise se is (A) Skin r sh (B) Exophth lmos (C) Hemolytic nemi (D) Ment l ret rd tion 39. G ucher’s dise se is due to deficiency of the enzyme: (A) (B) (C) (D) Sphingomyelin se Glucocere rosid se G l ctocer r osid se β-G l ctosid se 31. Free f tty cids re tr nsported in the lood (A) Com ined with l umin (B) Com ined with f tty cid inding protein (C) Com ined with β -lipoprotein (D) In u n ound free s lts 32. Long ch in f tty cids re first ctiv ted to cetyl-CoA i n (A) Cytosol (C) Nucleus (B) Microsomes (D) Mitochondri 40. Ch r cteristic finding in G ucher’s dise se is (A) (B) (C) (D) Night Ren l f ilure Hep tosplenomeg ly De fness 33. The enzyme cyl-CoA synth se c t lyses the conversion of f tty ctive f tty cid in the presence of (A) AMP (C) ATP (B) ADP (D) GTP
lindness
cid of
41. An import nt finding in Neim nn-Pick dise se is (A) (B) (C) (D) Leukopeni rdi c enl rgement Corne l op city Hep tosplenomeg ly
n C
34. C rnitine is synthesized from (A) Lysine nd methionine (B) Glycine nd rgi nine (C) Asp rt te nd glut m te (D) Proline nd hydroxyproline 35. The enzymes of β-oxid tion re found in (A) Mitochondri (B) Cytosol (C) Golgi pp r tus (D) N ucleus 36. Long ch in f tty cids penetr te the inner mitochondri l mem r ne (A) Freely (B) As cyl-CoA deriv tive (C) As c rnitine deriv tive (D) Requiring N dependent c rrier 37. An import nt fe ture of Zellweger’s syndrome is (A) Hypoglyc emi (B) Accumul tion of phyt nic cid in tissues (C) Skin eruptions (D) Accumul tion of C26-C38 polyenoic cid in r in tissues 42. Fucosidosis is ch r cterized y (A) Muscle sp sticity (B) Liver enl rgement (C) Skin r sh (D) Kidney f ilure 43. Met chrom tic leukodystrophy is due to defi ciency of enzyme: (A) α-Fucosid se (C) Cer mid se (B) Arylsulph t se A (D) Hexos m inid se A 44. A signific nt fe ture of T ngier dise se is (A) (B) (C) (D) Imp irment of ch ylomicron form tion Hypotri cylglycerolmi A sence of Apo-C-II A sence of Apo-CI 45. A signific nt fe ture of Bro d Bet dise se is (A) (B) (C) (D) Hypocholester olemi Hypotri cylglycerolemi A sence of Apo-D A norm lity of Apo-E
76 46. Neon t l tyrosinemi improves on e (B) Ri ofl vin (D) Ascor ic cid
dministr tion of (A) Thi min (C) Pyridoxin
MCQs IN BIOCHEMISTRY 54. Incre sed urin ry indole cetic cid is di gnostic of (A) (B) (C) (D) M ple syrup urine dise se H rtnup dise se Homocystinui Phenylketonuri 47. A sence of phenyl l nine hydroxyl se c uses (A) (B) (C) (D) Neon t l tyrosin emi Phenylketonuri Prim ry hyperox luri Al inism 55. In glycinuri d ily urin ry excretion of glycine r nges from (A) 100–200 mg (C ) 600–1000 mg (B) 300–500 mg (D) 1100–1400 mg 48. Richner-H nh rt syndrome is due to defect in (A) (B) (C) (D) Tyrosin se Phen yl l nine hydroxyl se Hep tic tyrosine tr ns min se Fum ryl ceto cet te hydrol s e 56. An in orn error, m ple syrup urine dise se is due to deficiency of the enzym e: (A) (B) (C) (D) Isov leryl-CoAhydrogen se Phenyl lnine hydroxyl se Adenosyl t r nsfer se α-Keto cid dec r oxyl se 49. Pl sm tyrosine level in Richner-H nh rt syndrome is (A) 1–2 mg/dL (C) 4–5 mg/dL (B) 2–3 mg/dL (D) 8–10 mg/dL 57. M ple syrup urine dise se ecomes evident in extr uterine life f (A) First week (C) Third week (B) Second week (D) Fourth week
y the end o
50. Amount of phenyl cetic cid excreted in the urine in phenylketonuri is (A) 100–200 mg/dL (B) 200–280 mg/dL (C) 290–550 mg/dL (D) 600–750 mg/dL 51. Tyrosinosis is d ue to defect in the enzyme: (A) (B) (C) (D) Fum ryl ceto cet te hydrol se p-Hydr oxyphenylpyruv te hydroxyl se Tyrosine tr ns min se Tyrosine hydroxyl se 58. Alk ptonuri occurs due to deficiency of the enzyme: (A) (B) (C) (D) M leyl ceto cet te isomer se Homogentis te oxid se p-Hydroxyphenylpyruv te hydroxyl se Fum ryl ceto cet te hydrol se 59. An import nt fe ture of m ple syrup urine dise se is (A) P tient c n not e tre ted y diet ry regul tion (B) Without tre tment de th, of p tient m y occur y the end of second ye r of life (C) Blood levels of leucine, isoleucine nd se rine re incre sed (D) Excessive r in d m ge 60. Ochronosis is n import nt fin ding of (A) (B) (C) (D) Tyrosinemi Tyrosinosis Alk ptonuri Richner H nh rt syn drome 52. An import nt finding in Histidinemi is (A) Imp irment of conversion of α-Glut m te to α-ketoglut r te (B) Speech defect (C) Decre sed urin ry histidine level ( D) P tients c n not e tre ted y diet 53. An import nt finding in glycinuri is (A) (B) (C) (D) Excess excretion of ox l te in the urine Deficiency of enzyme g lycin se Signific ntly incre sed serum glycine level Defect in ren l tu ul r re sorption of glycine
FATS AND FATTY ACID METABOLISM 61. Phrynoderm is deficiency of (A) Essenti l f tty cids(B) Proteins (C) Ami no cids (D) None of these 62. The percent ge of linoleic cid in s fflower oil is (A) 73 (C) 40 (B) 57 (D) 15 77 71. The deficiency of oth energy B) Kw shiork r (D) Beri- eri
nd protein c uses (A) M r smus (C) Di
etes (
72. Kw shiork r is ch r cterized y (A) Night lindness (B) Edem (C) E sy fr ct ur ility (D) Xerophth lmi 73. A ch r cteristic fe ture of Kw shiork r is (A) ( B) (C) (D) F tty liver Em ci tion Low insulin lever Occurrence in less th n 1 ye r inf nt 63. The percent ge of polyuns tur ted f tty 3 (B) 10 (D) 2
cids in soy
64. The percent ge of polyuns tur ted f tty 37 (D) 3
cids in
e n oil is (A) 62 (C)
utter is (A) 60 (C) 25 (B)
74. A ch r cteristic fe ture of m r smus is (A) (B) (C) (D) Severe hypo l uminem i Norm l epinephrine level Mild muscle w sting Low insulin nd high cortisol le vel 65. Diet ry fi re denotes (A) Undigested proteins (B) Pl nt cell components th t c nnot e digested y own enzymes (C) All pl nt cell w ll components (D) All no n digesti le w ter insolu le polys cch ride 66. A high fi re diet is ssoci ted with reduced incidence of (A) (B) (C) (D) C rdiov scul r dise se C.N.S. dise se Liver dise se Skin dise se (B) Glycogen (D) Proteoglyc ns (B) Pe s (D) Cornfl ke s (B) Atherosclerosis (D) Liver dise se 75. O esity gener lly reflects excess int ke of energy nd is often ssoci ted w ith the development of (A) (B) (C) (D) Nervousness Non-insulin dependent di ete s mellitus Hep titis Colon c ncer 76. Atherosclerosis nd coron ry he rt dise ses re ssoci ted with the diet: (A ) (B) (C) (D) High in tot l f t nd s tur ted f t Low in protein High in protein High in c r ohydr te 67. Diet ry fi res re rich in (A) Cellulose (C) St rch (A) Dried n (A) Peptic ulcer (C) Di etes
pricot (C) Br
68. Minimum diet ry fi re is found in 77. Cere rov sul r dise se nd hypertension is ssoci ted with (A) (B) (C) (D) H igh c lcium int ke High s lt int ke Low c lcium int ke Low s lt int ke 69. A
l nd diet is recommended in
78. The norm l r nge of tot l serum iliru in is (A) (B) (C) (D) 0.2–1.2 mg/100 ml 1.5–1.8 mg/100 ml 2.0–4.0 mg/100 ml A ove 7.0 mg/100 ml 70. A diet ry deficiency in oth the qu ntity nd the qu lity of protein results in (A) Kw shiork r (C) Xeropht lmi (B) M r smus (D) Liver dise ses
78 79. The norm l r nge of direct re cting (conjug ted) serum iliru in is (A) (B) (C) (D) 0–0.1 mg/100 ml 0.1–0.4 mg/100 ml 0.4–06 mg/100 ml 0.5–1 mg/100 ml MCQs IN BIOCHEMISTRY 86. Fec l sterco ilinogen is incre sed in (A) Hemolytic j undice (B) Hep tic j u ndice (C) Vir l hep titis (D) O structive j undice 87. Fec l uro ilinogen is inc re sed in (A) Hemolytic j undice (B) O struction of ili ry duct (C) Extr hep ti c g ll stones (D) Enl rged lymphnodes 88. A mixture of conjug ted nd unconjug t ed iliru in is found in the circul tion in (A) Hemolytic j undice (B) Hep tic j undice (C) O structive j undice (D) Post hep tic j undice 89. Hep tocellul r j undice s comp red to pure o structive type of j undice is ch r cterized y (A) Incre sed serum lk line phosph te, LDH nd ALT (B) Decre sed serum lk line pho sph t se, LDH nd ALT (C) Incre sed serum lk line phosph t se nd decre sed lev els of LDH nd ALT (D) Decre sed serum lk line phosph t se nd incre sed serum LDH nd ALT 90. Icteric index of n norm l dult v ries etween (A) 1–2 (C) 4–6 (B) 2–4 (D) 10–15 80. The norm l r nge of indirect (unconjug ted) iliru in in serum is (A) (B) (C ) (D) 0–0.1 mg/100 ml 0.1–0.2 mg/100 ml 0.2–0.7 mg/100 ml 0.8–1.0 mg/100 ml 81. J undice is visi le when serum iliru in exceeds (A) 0.5 mg/100 ml (C) 1 mg/ 100 ml (B) 0.8 mg/100 ml (D) 2.4 mg/100 ml 82. An incre se in serum unconjug ted iliru in occurs in (A) (B) (C) (D) Hemoly tic j undice O structive j undice Nephritis Glomerulonephritis 83. One of the c uses of hemolytic j undice is (A) (B) (C) (D) G-6 phosph t se d eficiency Incre sed conjug ted iliru in Glucokin se deficiency Phosphoglucomut se deficiency 84. Incre sed uro ilinogen in urine nd sence of iliru in in the urine sugges ts (A) (B) (C) (D) O structive j undice Hemolytic j undice Vir l hep titis Toxic j undice 91. Clinic l j undice is present with (D) 15
n icteric index
ove (A) 4 (C) 10 (B) 8
85. A j undice in which serum l nine tr ns min se nd lk line phosph t se re norm l is (A) (B) (C) (D) Hep tic j undice Hemolytic j undice P renchym tous j u ndice O structive J undice 92. Norm l qu ntity of uro ilinogen excreted in the feces per d y is 0–25 mg (C) 300–500 mg (B) 50–250 mg (D) 700–800 mg
out (A) 1
FATS AND FATTY ACID METABOLISM 93. Fec l uro ilinogen is decre sed in (A) (B) (C) (D) O struction of ct Hemolytic j undice Excess f t int ke Low f t int ke
ili ry du
79 100. The ility of liver to remove dye like BSP from the lood suggests nor m l (A) (B) (C) (D) Excretory function Detoxific tion function Met olic functio n Circul tory function 94. A complete sence of fec l uro ilinogen is strongly suggestive of (A) (B) ( C) (D) O struction of ile duct Hemolytic j undice Intr hep tic cholest sis M li gn nt o structive dise se 101. Remov l of BSP dye y the liver involves conjug tion with (A) (B) (C) (D) T hiosulph te Glut mine Cystein component of glut thione UDP glucuron te 95. Immedi te direct V nden Bergh re ction indic tes (A) (B) (C) (D) Hemolytic j undice Hep tic j undice O structive j undice Meg lo stic nemi 102. Norm l v lue of pl sm tot l proteins v ries etween (A) 3–4 gm/100ml (B) 6–8 g m/100ml (C) 10–12 gm/100ml (D) 14–16 gm/100ml 103. A decre se in l umin with incre sed production of other unidentified proteins which migr te in β, γ re ion su ests (A) (B) (C) (D) Cirrhosis of liver Nephrotic syndrome Infection Chronic lymphati c leukemia 96. The presence of bilirubin in the urine without urobilino en su ests (A) (B) (C) (D) Obstructive jaundice Hemolytic jaundice Pernicious anemia Dama e to the hepatic parenchyma 97. Impaired alactose tolerance test su ests (A) (B) (C) (D) Defect in utilisation Liver cell injury Renal defect Muscle injury
lucose
104. In increase in α 2-Glo ulin with loss of l umin in urine suggests (A) (B) (C ) (D) Prim ry immune deficiency Nephrotic syndrome Cirrhosis of liver Multiple m yelom 98. Incre sed serum ornithine c r moyl tr nsfer se ctivity is di gnostic of ( A) (B) (C) (D) Myoc rdi l inf rction Hemolytic j undice Bone dise se Acute vir l hep titis 105. The norm l levels of prothrom in time is (C) (D) (B) 4 sec (D) 10–16 sec
out (A) 2 sec (C) 14 sec (A) (B)
106. In o structive j undice prothrom in time Rem ins norm l Decre ses Responds to vit K nd ecomes norm l Responds to vit K nd incre ses 99. The est known nd most frequently used test of the detoxic ting functions o f liver is (A) (B) (C) (D) Hippuric cid test G l ctose toler nce test Epinephri ne toler nce test Rose Beng l dye test 107. In p renhym tous liver dise se the prothrom in time (A) Rem ins norm l (C) Decre ses (B) Incre ses (D) Responds to Vit K
80 108. Ure cle r nce test is used to determine the (A) (B) (C) (D) Glomerul r fil tr tion r te Ren l pl sm flow A ility of kidney to concentr te the urine Me sur ement of tu ul r m ss MCQs IN BIOCHEMISTRY 116. Me surement of insulin cle r nce test is me sure of (A) (B) (C) (D) Glome rul r filtr tion r te Filtr tion f ctor Ren l pl sm flow Tu ul r secretory m ss 109. The formul to c lcul te m ximum ure U× V cle r nce is , where U denotes B 117. The polys cch ride insulin is (A) Filtered t the glomerulus ut neither se creted nor re sor ed y the tu ule (B) Filtered t the glomerulus nd secreted y the tu ule (C) Filtered t the glomerulus nd re sor ed y the tu ule (D) Fi ltered t the glomerulus, secreted nd re sor ed y the tu ule 118. Norm l insu lin cle r nce is (A) 40 ml/1.73 sqm (B) 60 ml/1.73 sqm (C) 80 ml/1.73 sqm (D) 12 0 ml/1.73 sqm 119. Cre tinine EDTA cle r nce is test to me sure (A) (B) (C) (D ) Ren l pl sm flow Filtr tion fr ction Glomerul r filtr tion r te Tu ul r funct ion (B) cid (D) Both (A) nd (C) (A) (B) (C) (D) Concentr tion of ure in urine in gm/24 hr Concentr tion of ure in urine in mg/ 100 ml Concentr tion of ure in lood in mg/100 ml Volume of urine in ml/mt 110. Aver ge m ximum ure
cle r nce is (A) 30 ml (C) 75 ml (B) 50 ml (D) 90 ml
111. The ver ge norm l v lue for st nd rd ure (B) 30 ml (D) 54 ml
cle r nce is (A) 20 ml (C) 40 ml
112. Ure cle r nce is lowered in (A) (B) (C) (D) Acute nephritis Pneumoni y st ge of nephritic syndrome Benign hypertension
E rl
120. The end products of s ponific tion: (A) glycerol (C) so p 113. Glomerul r filtr tion r te c n e me sured y (A) (B) (C) (D) Endogenous cr e tinine cle r nce P r - minohippur te test Addis test Mosenth l test 121. The norm l PAH cle r nce for surf ce re (C) 400 ml/min (B) 300 ml/min (D) 574 ml/min
of 1.73 sqm. is (A) 200 ml/min
122. P r mino hippur te is (A) Filtered t glomeruli nd secreted y the tu ul es (B) Filtered t glomeruli nd not secreted y the tu ules (C) Filtered t glo meruli nd re sor ed completely (D) Not removed completely during single circ ul tion of the lood through the kidney. 123. The Tm for PAH i.e the m xim l sec retory c p city of the tu ule for PAH c n e used to g vge the (A) Extent of tu ul r d m ge 114. At norm l levels of cre tinine in the lood, this met olite is (A) Filtere d t the glomerulus ut not secreted nor re sor ed y the tu ule (B) Secreted y the tu ule (C) Re sor ed y the tu ule (D) Secreted nd re sor ed y tu ule 115. The norm l v lues for cre tinine cle r nce v ries from (A) 20–40 ml/min (C) 7 0–85 ml/min (B) 40–60 ml/min (D) 95–105 ml/min
FATS AND FATTY ACID METABOLISM (B) Imp irment of the c p city of the tu ule to perform osmotic work (C) Imp irm ent of ren l pl sm flow (D) Glomerul r filtr tion r te 124. The norm l Tm in mg /min/1.73 sqm for PAH is (A) 20 (C) 60 (B) 40 (D) 80 132. Triglycerides re (A) (B) (C) (D) He vier th n w ter M jor constituents of mem r nes Non-pol r Hydroph ilic 81 133. Cere ronic cid is present in (A) (B) (C) (D) Glycerophospholipids Sphingop hospholipids G l ctosyl cer mide G ngliosides 125. The norm l r nge of filtr tion f ctor in 0 (B) 0.16–0.21 (D) 0.35–0.40 134. Acylsphingosine is mide (D) Sulph tide
lso known
n
dult is (A) 0.10–0.15 (C) 0.25–0.3
s (A) Sphingomyelin (C) Cere roside (B) Cer
126. The filtr tion f ctor tends to e norm l in (A) (B) (C) (D) E rly essenti l hypertension M lign nt ph se of hypertension Glomerulonephritis Acute nephritis 135. The highest phospholipids content is found in (A) Chylomicrons (C) LDL (B) VLDL (D) HDL 127. The filtr tion f ctor is incre sed in (A) (B) (C) (D) Glomerulonephritis M lign nt ph se of hypertension E rly essenti l hypertension Acute nephritis 136. The m jor lipid in chylomicrons is (A) Triglycerides (C) Cholesterol (B) Ph ospholipids (D) Free f tty cids 137. Num er of c r on
toms in cholesterol is (A) 17 (C) 27 (B) 19 (D) 30
128. The filtr tion f ctor is decre sed in (A) (B) (C) (D) Glomerulonephritis E rly essenti l hypertension M lign nt ph se of hypertension St rv tion 138. The lipoprotein richest in cholesterol is (A) Chylomicrons (C) LDL (B) VLDL (D) HDL 129. Excretion of phenolsulph npth lein (PSP) reflects (A) (B) (C) (D) Glomerulo nephritis M xim lt ul r excretory c p city Filtr tion f ctor Ren l pl sm flow 139. The m jor stor ge form of lipids is (A) (B) (C) (D) Esterified cholesterol Glycerophospholipids Triglycerides Sphingolipids 130. Which of the following is polyuns tur ted f tty C) Linoleic cid (B) P lmitoleic cid (D) Oleic cid
cid? (A) P lmitic
cid (
140. Cere onic cid is present in (A) (B) (C) (D) Triglycerides Cere rosides Est erified cholestrol Sphingomyelin 131. Which of the following is omeg -3 polyuns tur ted f tty cid? (A) Linoleic cid (C) γ Linolenic acid (B) α-Linolenic cid (D) Ar chidonic cid 141. The nitrogenous (D) Bet ine
se in lecithin is (A) Eth nol mine (C) Serine (B) Choline
82 142. All the following re omeg -6-f tty cids except (A) Linoleic lenic acid (B) α-Linolenic cid (D) Ar chidonic cid MCQs IN BIOCHEMISTRY 152. De hovo synthesis of f tty itochondri (D) All of these
cid (C) γ Lino
cids occurs in (A) Cytosol (C) Microsomes (B) M
143. All the following h ve 18 c r on toms except (A) Linoleic cid (B) Linolen ic cid (C) Ar chidonic cid (D) Ste ric cid 144. A 20-c r on f tty cid mong the following is (A) Linoleic cid (C) β -Linolenic cid (B) α -Linolenic cid (D) A r chidonic cid 153. Acyl C rrier Protein cont ins the vit min: (A) Biotin (B) Lipoic cid (C) P ntothenic cid (D) Folic cid 154. Which of the following is required s redu ct nt in f tty cid synthesis? (A) NADH (C) FADH2 (A) cAMP (C) Epinephrine (B) N ADPH (D) FMNH2 (B) Gluc gon (D) Insulin 155. Hep tic liponenesis is stimul ted
y:
145. Triglycerides re tr nsported from liver to extr hep tic tissues lomicrons (C) HDL (B) VLDL (D) LDL
y (A) Chy
156. De novo synthesis of f tty cids requires ll of the following except (A) B iotin (B) NADH (C) P nthothenic cid (D) ATP 157. Acetyl CoA c r oxyl se regul t es f tty cid synthesis y which of the following mech nism? (A) (B) (C) (D) All osteric regul tion Cov lent modific tion Induction nd repression All of these 146. Cholesterol is tr nsported from liver to extr hep tic tissues icrons (C) HDL (B) VLDL (D) LDL 147. Elev ted pl sm level of the following projects ) Chylomicrons (C) HDL (B) VLDL (D) LDL
g inst
y (A) Chylom
therosclerosis: (A
148. All the following mino cids re nonessenti l except (A) Al nine (C) Cyste ine (A) Cysteine (C) Both (A) nd (B) (B) Histidine (D) Proline (B) Methionine ( D) None of these 158. β-Oxid tion of f tty cids requires A (C) NAD (B) FAD (D) NADP
ll the following coenzymes except (A) Co
149. Sulphydryl group is present in 159. Which of the following c n e oxidized ) S tur ted f tty cids Monos tur ted f tty l of these
y β-oxid tion p thw y? (A) (B) (C) (D cids Polyuns tur ted f tty cids Al
150. Oligos cch ride-pyrophosphoryl dolichol is required for the synthesis of (A ) (B) (C) (D) N-linked glycoproteins O-linked glycoproteins GPI-linked glycoprot eins All of these 160. Propionyl CoA is formed on oxid tion of (A) (B) (C) (D) Monouns tur ted f t ty cids Polyuns tur ted f tty cids F tty cids with odd num er of c r on toms None of these 151. In N-linked glycoproteins, oligos cch ride is tt ched to protein through i ts (A) Asp r gine residue (B) Glut mine residue (C) Arginine residue (D) Lysine residue
FATS AND FATTY ACID METABOLISM 161. An enzyme required for the synthesis of ketone odies s well s cholestero l is (A) (B) (C) (D) Acetyl CoA c r oxyl se HMG CoA synthet se HMG CoA reduct se HMG CoA ly se (B) Liver (D) Br in 169. Throm ox nes c use (A) (B) (C) (D) V sod il tion Bronchoconstriction Pl telet ggreg tion All of these 83 162. Ketone
odies
re synthesized in (A) Adipose tissue (C) Muscles
170. Prost gl ndins lower c mp in (A) Adipose tissue (C) Pl telets (B) Lungs (D) Adenohypophysis 163. All the following st tements out ketone odies re true except (A) (B) (C ) (D) Their synthesis incre ses in di etes mellitus They re synthesized in mit chondri They c n deplete the lk li reserve They c n e oxidized in the liver 171. Slow re cting Su st nce of An phyl xis is mixture of (A) Prost gl ndins ( C) Throm ox nes (A) (B) (C) (D) (B) Prost cyclins (D) Leukotrienes 172. Dip lmitoyl lecithin cts s Pl telet ctiv ting f ctor Second messenger fo r hormones Lung surf ct nt Anti-ketogenic compound (B) 0.5 KOH (D) 0.5 N OH 164. All the following st tements out c rnitine re true except (A) It c n e synthesised in the hum n ody (B) It c n e synthesized from methionine nd lysi ne (C) It is required for tr nsport of short ch in f tty cids into mitochondri (D) Its deficiency c n occur due to h emodi lysis 165. Which of the following c n e synthesized in the hum n ody if precurors re v il le? (A) Oleic cid ( B) P lmitoleic cid (C) Ar chidonic cid (D) All of these 166. All the following c n e oxidized y βoxid tion except (A) (B) (C) (D) P lmitic cid Phyt nic cid Linoleic cid F tty cids h ving n odd num er of c r on toms 173. Reichert-Meissl num er: (A) 0.1 N KOH (C) 0.1 N N OH 174. In glycerophospholipids, polyuns tur ted f tty cid is commonly tt ched to which of the following c r on tom of glycerol? (A) C r on 1 (C) Both (A) nd (B) (B) C r on 2 (D) None of these 175. Lysolecithin is formed from lecithin y remov l of (A) (B) (C) (D) (A) (B) (C) (D) F tty cid from position 1 F tty cid from position 2 Phosphorylcholine Choline P lmitoyl CoA nd Choline P lmitoyl CoA nd eth nol mine P lmitoyl CoA nd serine Acetyl CoA nd choline 176. Sphingosine is synthesized from 167. Anti-infl mm tory corticosteroids inhi it the synthesis of (A) Leukotrienes (C) Throm ox nes (B) Prost gl ndins (D) All of these 168. Diets h ving high r tio of polyuns tur ted: s tur ted f tty cids c n c u se (A) (B) (C) (D) Incre se in serum triglycerides Decre se in serum cholesterol Decre se in serum HDL Skin lesions 177. For synthesis of sphingosine, ll the following coenzymes pt (A) (B) (C) (D) Pyridox l phosph te NADPH FAD NAD
re required exce
84 178. Cere rosides cont in ll the following except (A) G l ctose (C) Sphingosine (B) Sulph te (D) F tty cid MCQs IN BIOCHEMISTRY 188. Activ ted lecithin cholesterol cyl tr nsfer se is essenti l for the conver sion of (A) (B) (C) (D) VLDL remn nts into LDL N scent HDL into HDL HDL2 into HD L3 HDL3 into HDL2 Deficiency of methionine Puromycin Chronic lcoholism All of t hese 179. Niem nn-Pick dise se results from deficiency of (A) Cer mid se (B) Sphingom yelin se (C) Arylsulph t se A (D) Hexos minid se A 180. Chylomicron remn nts re c t olised in (A) Intestine (C) Liver (B) Adipose tissue (D) Liver nd intesti ne 189. F tty liver m y 181. VLDL remn nt m y s
e c used
y (A) (B) (C) (D)
e converted into (A) VLDL (C) HDL (B) LDL (D) Chylomicron
190. Alcohol dehydrogen se converts eth nol into (A) Acetyl CoA (C) Acet te (B) Acet ldehyde (D) CO2 nd H2O 182. Receptors for chylomicron remn nts re (A) Apo A specific (C) Apo C specifi c (B) Apo B-48 specific (D) Apo E specific 191. Lipids re stored in the ody m inly in the form of (A) Phospholipids (C) T riglycerides (B) Glycolipids (D) F tty cids 183. LDL receptor is specific for (A) (B) (C) (D) Apo B-48 8 nd Apo E Apo B-100 nd Apo D Apo B-100 nd po D 192. Lipid stores ose tissue
nd Apo B 100 Apo B-4
re m inly present in (A) Liver (C) Muscles (B) Br in (D) Adip
184. N scent HDL of intestin l srcin l o C nd Apo E
cks (A) Apo A (C) Apo E (B) Apo C (D) Ap
193. Glycerol is converted into glycerol-3phosph te ol kin se (B) Triokin se (D) All of these
y (A) Thiokin se (C) Glycer
185. HDL is synthesized in (A) Adipose tissue (C) Intestine (B) Liver (D) Liver nd intestine 194. In dipose tissue, glycerol-3-phosph te required for the synthesis of trigl ycerides comes m inly from (A) Hydrolysis of pre-existing triglycerides (B) Hydr olysis of phospholipids (C) Dihydroxy cetone phosph te formed in glycolysis (D) Free glycerol 195. Glycerol rele sed from dipose tissue y hydrolysis of trigly cerides is m inly (A) (B) (C) (D) T ken up y liver T ken up y extr hep tic tis sues Reutilised in dipose tissue Excreted from the ody 186. N scent HDL of intestin l srcin cquires Apo C (D) Chylomicrons VLDL LDL HDL of the hep tic srcin
nd Apo E from (A) (B) (C)
187. Hep rin rele s le hep tic lip se converts (A) (B) (C) (D) VLDL remn nts in to LDL N scent HDL into HDL HDL2 into HDL3 HDL3 into HDL2
FATS AND FATTY ACID METABOLISM 196. Free glycerol c nnot e used for triglyceride synthesis in (A) Liver (C) In testine (B) Kidney (D) Adipose tissue 204. Oxid tion of f tty cids occurs (A) ( B) (C) (D) In the cytosol In the m trix of mitochondri On inner mitochondri l m em r ne On the microsomes 85 197. Adipose tissue l cks (A) (B) (C) (D) Hormone-sensitive lip se Glycerol kin se cAMP-dependent protein kin se Glycerol-3-phosph te dehydrogen se 205. Activ tion of f tty cids requires okin se (B) Coenzyme A (D) C rnitine
ll the following except (A) ATP (C) Thi
198. A digestive secretion th t does not cont in ny digestive enzyme is (A) S l iv (C) P ncre tic juice (B) G stric juice (D) Bile 206. Mitochondri l thiokin se cts on (A) (B) (C) (D) Short ch in of f tty Medium ch in f tty cids Long ch in f tty cids All of these
cids
199. S liv cont ins lip se which cts on triglycerides h ving (A) (B) (C) (D) Short ch in f tty cids Medium ch in f tty cids Long ch in f tty cids All of these 207. C rnitine is required for the tr nsport of (A) (B) (C) (D) Triglycerides ou t of liver Triglycerides into mitochondri Short ch in f tty cids into mitochon dri Long ch in f tty cids into mitochondri 200. S liv ry lip se hydrolyses the ester ond t (A) (B) (C) (D) Position 1 of triglycerides Position 2 of triglycerides Position 3 of triglycerides All of the se 208. C rnitine cylc rnitine tr nsloc se is present (A) In the inner mitochondri l mem r ne (B) In the mitochondri l m trix (C) On the outer surf ce of inner mi tochondri l mem r ne (D) On the inner surf ce of inner mitochondri l mem r ne 20 9. Net ATP gener tion on complete oxid tion of ste ric cid is (A) 129 (C) 146 ( B) 131 (D) 148 201. S liv ry lip se converts diet ry triglycerides into (A) (B) (C) (D) Diglyce rides nd f tty cids Monoglycerides nd f tty cids Glycerol nd f tty cids Al l of these 202. P ncre tic lip se requires for its s (B) Bile s lts (D) All of these
ctivity: (A) Co-lip se (C) Phospholipid
210. Propionyl CoA formed oxid tion of f tty cids h ving n odd num er of c r o n toms is converted into (A) (B) (C) (D) Acetyl CoA Aceto cetyl CoA D-Methylm l onyl CoA Butyryl CoA 203. P ncre tic lip se converts tri cylglycerols into (A) (B) (C) (D) 2, 3-Di cy lglycerol 1-Mono cylglycerol 2-Mono cylglycerol 3-Mono cylglycerol 211. α-Oxid tion of f tty D) Adipose tissue
cids occurs m inly in (A) Liver (C) Muscles (B) Br in (
86 212. Refsum’s dise se results from defect in the following p thw y except (A) Al ph -oxid tion of f tty cids (B) Bet -oxid tion of f tty cids (C) G mm -oxid ti on of f tty cids (D) Omeg -oxid tion of f tty cids 213. The end product of ome g -oxid tion of f tty cids h ving n even num er of c r on toms is (A) Adipic cid (C) Both (A) nd (B) (B) Su eric cid (D) None of these MCQs IN BIOCHEMISTRY 220. During e ch cycle of β-oxid tion (A) One c r on tom is removed from the c r oxyl end of the f tty cid (B) One c r on tom is removed from the methyl end of the f tty cid (C) Two c r on toms re removed from the c r oxyl end of the f tty cid (D) Two c r on toms re removed from the methyl end of the f tty cid 221. Net gener tion of energy on complete oxid tion of p lmitic cid is (A) (B) (C) (D) 129 ATP equiv lents 131 ATP equiv lents 146 ATP equiv lents 148 ATP equi v lents 214. De novo synthesis of f tty cids is c t lysed y multi-enzyme complex whi ch cont ins (A) One-SH group (B) Two-SH groups (C) Three-SH groups (D) Four-SH g roups 215. F t depots re loc ted in (A) Intermuscul r connective tissue (B) Mes ent ry (C) Omentum (D) All of these 216. S liv ry lip se is secreted y (A) P ro tid gl nds (B) Su -m xill ry gl nds (C) Dors l surf ce of tongue (D) None of the se 217. Co-lip se is (A) Bile s lt (C) Protein (B) Vit min (D) Phospholipid 222. Net gener tion of energy on complete oxid tion of 17-c r on f tty cid is (A) Equ l to the energy gener tion from 16-c r on f tty cid (B) Equ l to the energy gener tion from n 18-c r on f tty cid (C) Less th n the energy gener t ion from 16-c r on f tty cid (D) In etween the energy gener tion from 16-c r on f tty cid nd n 18-c r on f tty cid 223. Net energy gener tion on compl ete oxid tion of linoleic cid is (A) (B) (C) (D) 148 ATP equiv lents 146 ATP eq uiv lents 144 ATP equiv lents 142 ATP equiv lents 218. Pl LDL (C) to (A) y cids
sm ecomes milky (A) Due to high level of HDL (B) Due to high level of During f sting (D) After me l 219. Mitochondri l mem r ne is perme le Short ch in f tty cids (B) Medium ch in f tty cids (C) Long ch in f tt (D) All of these
224. Extr mitochondri l synthesis of f tty cids occurs in (A) M mm ry gl nds (B ) Lungs (C) Br in (D) All of these 225. One function l su -unit of multi-enzyme complex for de novo synthesis of f tty cids cont ins (A) (B) (C) (D) One —SH grou p Two —SH groups Three —SH groups Four —SH groups
FATS AND FATTY ACID METABOLISM 226. NADPH required for f tty cid synthesis c n come from (A) (B) (C) (D) Hexos e monophosph te shunt Oxid tive dec r oxyl tion of m l te Extr mitochondri l oxi d tion of isocitr te All of these 87 234. Lipo-oxygen se is required for the synthesis of (A) Prost gl ndins (C) Thro m ox nes (B) Leukotrienes (D) All of these 235. All of the following st tements out multiple sclerosis re true except (A ) There is loss of phospholipids from white m tter (B) There is loss of sphingol ipids from white m tter (C) There is loss of esterified cholesterol from white m tter (D) White m tter resem les gr y m tter in composition 236. After entering cytosol, free f tty cids re ound to (A) Al umin (C) Z-protein (B) Glo ulin (D ) None of these 227. F tty liver m y e prevented y ll of the following except (A) Choline (C) Methionine (B) Bet ine (D) Ethionine 228. Hum n des tur se enzyme system c nnot introduce dou le id eyond (A) C r on 9 (C) C r on 5 (B) C r on 6 (D) C r on 3
ond in
f tty
c
229. Which of the following lipid is sor ed ctively from intestines? (A) (B) (C) (D) Glycerol Cholesterol Mono cylglycerol None of these 237. Rele se of free f tty cids from dipose tissue is incre sed y ll of the following except (A) Gluc gon (B) Epinephrine (C) Growth hormone (D) Insulin 238 . All the following st tements out rown dipose tissue re true except (A) It is rich in cytochromes (B) It oxidizes glucose nd f tty cids (C) Oxid tion n d phosphoryl tion re tightly coupled in it (D) Dinitrophenol h s no effect on i t 239. Lov st tin nd mev st tin lower (A) (B) (C) (D) Serum triglycerides Serum cholesterol Serum phospholipids All of these 230. C22 nd C24, f tty cids required for the synthesis of sphingolipids in r in re formed y (A) (B) (C) (D) De novo synthesis Microsom l ch in elong tion M itochondri l ch in elong tion All of these 231. Sphingomyelins: (A) Phospholipids (C) Alcohols (B) Nitrolipids (D) None of these 232. All of the following st tements out hypoglycin re true except (A) (B) (C ) (D) It is pl nt toxin It c uses hypoglyc emi It inhi its oxid tion of short ch in f tty cids It inhi its oxid tion of long ch in f tty cids 240. Lov st tin is (A) Competitive inhi itor of cetyl CoA c r oxyl se (B) Com petitive inhi itor of HMG CoA synthet se (C) Non-competitive inhi itor of HMG Co A reduct se (D) Competitive inhi itor of HMG CoA reduct se 241. A et lipoprotein emi occurs due to lock in the synthesis of (A) Apoprotein A (C) Apoprotein C (B) Apoprotein B (D) Cholesterol 233. Synthesis of prost gl ndins is inhi ited h cin (B) Aspirin (D) All of these
y (A) Glucocorticoids (C) Indomet
88 242. All of the following st tements out T ngier dise se re true except (A) ( B) (C) (D) It is disorder of HDL met olism Its inherit nce is utosom l reces sive Apoproteins A-I nd A-II re not synthesised Pl sm HDL is incre sed MCQs IN BIOCHEMISTRY (B) S tur ted f tty cids (C) Integr l proteins (D) Cholesterol 251. Tr nsition temper ture of mem r nes m y e ffected y the following constituent of mem r n es: (A) Peripher l proteins (B) Integr l proteins (C) Cholesterol (D) Oligos chh rides 252. Acetyl CoA formed from pyruv te c n e used for the synthesis of ll the following except (A) Glucose (C) Cholesterol (B) F tty cids (D) Steroid ho rmones 243. Genetic deficiency of lipoprotein lip se c uses hyperlipoprotein emi llowing type: (A) Type I (C) Type II (B) Type II (D) Type V
of fo
244. Chylomicrons re present in f sting lood s mples in hyperlipoprotein emi of following types: (A) Types I nd II (C) Types I nd V (B) Types II nd II (D) Types IV nd V 253. Which of the following c n e used s source of energy in extr hep tic ti ssues? (A) Aceto cet te (C) Both (A) nd (B) (B) Acetone (D) None of these 245. Glut thione is constituent of (A) Leukotriene A4 (C) Leukotriene C4 (A) ( B) (C) (D) (B) Throm ox ne A1 (D) None of these 254. Anti-infl mm tory corticosteroids inhi it (A) Phospholip se A1 (B) Phosphol ip se A2 (C) Cyclo-oxygen se (D) Lipo-oxygen se 255. Cyclo-oxygen se is involved in the synthesis of (A) Prost gl ndins (C) Both (A) nd (B) 256. Leukotrienes c use (A) (B) (C) (D) Incre se in c pill ry perme ility Aggreg tion of pl telets Bronchodil t tion None of these (B) Throm ox nes (D) None of these 246. Prost gl ndins re in ctiv ted y 15-Hydroxyprost gl ndin dehydrogen se Cyc lo-oxygen se Lipo-oxygen se None of these 247. Phenyl ut zone nd indometh cin inhi it (A) Phospholip se A1 (B) Phospholip se A2 (C) Cyclo-oxygen se (D) Lipo-oxygen se 248. Prost gl ndins stimul te (A) (B) (C) (D) Aggreg tion of pl telets Lipolysis in dipose tissue Bronchodil t ti on G stric cid secretion 257. Prost gl ndins decre se ll of the following except (A) (B) (C) (D) G stric cid secretion Blood pressure Uterine contr ction Pl telet ggreg tion 249. For extr mitochondri l f tty cid synthesis, cetyl CoA m y m (A) Citr te (C) Ox lo cet te (B) Isocitr te (D) Succin te
e o t ined fro
258. Hypocholesterol emi c n occur in (A) (B) (C) (D) Hyperthyroidism Nephrotic syndrome O structive j undice Di etes mellitus 250. Fluidity of mem r nes is incre sed Polyuns tur ted f tty cids
y the following constituent except (A)
FATS AND FATTY ACID METABOLISM 259. De novo synthesis nd oxid tion of f tty cids differ in the following resp ect: (A) Synthesis occurs in cytosol nd oxid tion in mitochondri (B) Synthesis is decre sed nd oxid tion incre sed y insulin (C) NADH is required in synthes is nd FAD in oxid tion (D) M lonyl CoA is formed during oxid tion ut not durin g synthesis 260. Free f tty cids rele sed from dipose tissue re tr nsported i n lood y (A) Al umin (C) LDL 261. (B) VLDL (D) HDL 89 266. The enzyme deficient in F ry’s dise se is (A) α-G l ctosid se (B) β-G l ctosid s e (C) α-Glucosid se (D) β-Glucosid se 267. Highest protein content mongst the follo wing is present in (A) Whe t (C) Pulses (B) Rice (D) Soy e n 268. D ily protein requirement of n dult m n is (A) (B) (C) (D) 0.5 gm/kg of ody weight 0.8 gm/kg of ody weight 1.0 gm/kg of ody weight 1.5 gm/kg of ody w eight β -G l ctosid se is deficient in (A) (B) (C) (D) F
ry’s dise se Kr
e’s dise se G ucher’s dise se Met chrom tic leukodystrophy
269. D ily protein requirement of n dult wom n is (A) (B) (C) (D) 0.5 gm/kg of ody weight 0.8 gm/kg of ody weight 1.0 gm/kg of ody weight 1.5 gm/kg of ody weight 262. The enzyme deficient in met chrom tic leukodystrophy is (A) Arylsulph t se A (B) Hexos minid se A (C) Cer mid se (D) Sphingomyelin se 263. All of the follo wing st tements out gener lized g ngliosidosis re true except (A) It results from deficiency of G M1 -βG ngliosid se (B) Bre kdown of GM1 g nglioside is imp ir ed (C) GM2 g nglioside ccumul tes in liver nd elsewhere (D) It le ds to ment l ret rd tion 264. Hexos minid se A is deficient in (A) (B) (C) (D) T y-S chs dis e se G ucher’s dise se Niem nn-Pick dise se F ry’s dise se 270. Cysteine c n p rti lly meet the requirement of (A) Phenyl l nine (C) Methio nine (B) Threonine (D) None of these 271. Invisi le f t is present in (A) Milk (C) Groundnut oil (A) Milk (C) Coconut oil (B) Coconut oil (D) Hydrogen ted oils (B) Pulses (D) Egg yolk 272. Visi le f t is present in 273. F t content of eggs is (B) 10% (D) 20% 274. F t content of pulses is
out (A) 7% (C) 13% (A) 5% (C) 15% (B) 10% (D) 16% out
265. Ment l ret rd tion occurs in (A) (B) (C) (D) T y-S chs dise se G ucher’s dise se Niem nn-Pick dise se All of these 275. Predomin nt f tty cids in me t re (A) (B) (C) (D) S tur ted Monouns tur t ed Polyuns tur ted Mono nd poly-uns tur ted
90 276. Oils h ving more th n 50 % polyuns tur ted f tty cids include ll of the f ollowing except (A) Groundnut oil (C) Sunflower oil (B) Soy e n oil (D) S fflow er oil MCQs IN BIOCHEMISTRY 286. In Ames’ ss y, ddition of c rcinogen to the culture medium llows S. typh imurium to grow (A) In the presence of histidine (B) In the presence of rginine (C) In the sence of histidine (D) In the sence of rginine 287. In Ames’ ss y, liver homogen te is included in the culture medium ec use (A) It converts pr o-c rcinogens into c rcinogens (B) Liver c n met olise histidine (C) S lmonell m inly infects liver (D) Liver is very suscepti le to c ncer 288. Bile pigments re present nd uro ilinogen sent in urine in (A) H emolytic j undice (B) Hep tocellul r j undice (C) O structive j undice (D) Crigler-N jj r syndrome 289. B ile pigments re sent nd uro ilinogen incre sed in urine in (A) H emolytic j undice (B) Hep tocellul r j undice (C) O structive j undice (D) Rotor’s syndrome 2 90. In o structive j undice, urine shows (A) A sence of ile pigments nd uro il inogen (B) Presence of ile pigments nd uro ilinogen (C) A sence of ile pigmen ts nd presence of uro ilinogen (D) Presence of ile pigments nd sence of uro ilinogen 291. In h emolytic j undice, urine shows (A) A sence of ile pigments nd uro ilinogen (B) Presence of ile pigments nd uro ilinogen (C) A sence of ile pigments nd presence of uro ilinogen (D) Presence of ile pigments nd se nce of uro ilinogen 277. Cholesterol is present in Fish (D) Pulses
ll of the following except (A) Egg (C) Milk (B)
278. Which of the following h s the highest cholesterol content? (A) Me t (C) Bu tter (B) Fish (D) Milk 279. Which of the following h s the highest cholesterol content? (A) Egg yolk (C ) Me t (B) Egg white (D) Fish 280. The following cont ins the le st cholesterol: (A) Milk (C) Butter (B) Me t (D) Cheese 281. Which of the following constitutes fi re or rough ge in food? (A) Cellulose (C) Inulin (B) Pectin (D) All of these 282. The st rch content of whe t is 283. The st rch content of pulses is 284. A signific nt source of st rch ) Spin ch (D) C uliflower
out (A) 50% (C) 70% (B) 60% (D) 80% out (A) 50% (C) 70% (B) 60% (D) 80% mong veget
les is (A) R dish (C) Pot to (B
285. The cyclic ring present in ll the steroids: (A) (B) (C) (D) Cyclopent no p erhydrophen nthrene Nitropent no oth (A) nd (B) None of these
FATS AND FATTY ACID METABOLISM 292. Serum l umin m y e decre sed in (A) (B) (C) (D) H emolytic j undice Hep t ocellul r j undice O structive j undice All of these (B) 2.0–3.6 mg/dl (D) 3.5–5.5 m g/dl (B) 2.0–3.6 gm/dl (D) 3.5–5.5 gm/dl 91 301. All the following st tements out o structive j undice re true except (A) Prothrom in time m y e prolonged due to imp ired sorption of vit min K (B) S erum lk line phosph t se m y e r ised due to incre sed rele se of the enzyme f rom liver cells (C) Bile s lts m y enter systemic circul tion due to ili ry o s truction (D) There is no defect in conjug tion of iliru in 302. A test to ev lu te detoxifying function of liver is (A) (B) (C) (D) (A) (B) (C) (D) (A) (B) (C) (D) Serum l umin: glo ulin r tio G l ctose toler nce test Hippuric cid test P rothrom in time Benzoic cid nd l nine Benzoic cid glycine Glucuronic cid n d l nine Glucuronic cid nd glycine L ct se dehydrogen se Amyl se Ornithine tr nsc r moyl se None of these 293. Norm l r nge of serum mg/dl (C) 3.5–5.5 mg/dl
l umin is (A) 2.0–3.6 gm/dl (C) 3.5–5.5 gm/dl (A) 2.0–3.6
294. Norm l r nge of serum glo ulin is 295. Serum l umin: glo ulin r tio is ltered in (A) Gil ert’s dise se (B) H emoly tic j undice (C) Vir l hep titis (D) Stones in ile duct 296. Esterific tion of cholesterol occurs m inly in (A) Adipose tissue (C) Muscles (A) (B) (C) (D) (A) (B) (C) (D) (B) Liver (D) Kidneys 303. Hippuric
cid is formed from
297. G l ctose intoler nce c n occur in H emolytic j undice Hep tocellul r j und ice O structive j undice None of these Erythrocytes Reticulo-endotheli l cells L iver Kidneys 304. An enzyme which is excreted in urine is 298. Prothrom in is synthesised in 305. Serum g mm glut myl tr nspeptid se is r ised in (A) (B) (C) (D) (A) (B) (C ) (D) (A) (B) (C) (D) H emolytic j undice Myoc rdi l inf rction Alcoholic hep ti tis Acute cholecystitis Di etes mellitus Di etes insipidus Acute glomeruloneph ritis Chronic glomerulonephritis Amount of ure excreted per minute Amount of ur e present in 100 ml of urine Volume of lood cle red of ure in one minute Amou nt of ure filtered y glomeruli in one minute 299. Prothrom in time rem ins prolonged even fter p renter ls dministr tion of vit min K in (A) (B) (C) (D) H emolytic j undice Liver d m ge Bili ry o structi on Ste torrhoe 306. Oliguri
c n occur in
300. All the following st tements out o structive j undice re true except (A) (B) (C) (D) Conjug ted iliru in in serum is norm l Tot l iliru in in serum is r ised Bile s lts re present in urine Serum lk line phosph t se is r ised 307. Ure
cle r nce is the
92 308. Inulin cle r nce is me sure of (A) (B) (C) (D) Glomerul r filtr tion r te Tu ul r secretion flow Tu ul r re sorption r te Ren l pl sm flow MCQs IN BIOCHEMISTRY 316. Esters of f tty cids with higher lcohols other th n glycerol e (A) W xes (C) Both (A) nd (B) (B) F ts (D) None of these
re s id to
309. Phenolsulphonephth lein excretion test is n indic tor of (A) (B) (C) (D) G lomerul r filtr tion Tu ul r secretion Tu ul r re sorption Ren l lood low 317. The com in tion of n mino lcohol, f tty cid nd si lic cid form (A) Ph ospholipids (C) Glycolipids (A) (B) (C) (D) (B) Sulpholipids (D) Aminolipids 318. Hydrolysis of f ts y lk li is c lled S ponific tion num er S ponific tion Both (A) nd (B) None of these 310. P r - mino hippur te excretion test is n indic tor of (A) (B) (C) (D) Glom erul r filtr tion Tu ul r secretion Tu ul r re sorption Ren l pl sm flow 319. The num er of milliliters of 0.1 N KOH required to neutr lize the insolu le f tty cids from 5 gms of f t is c lled (A) Acid num er (C) H logen tion (B) Ac etyl num er (D) Polenske num er 311. Ren l pl sm flow of n ver ge dult m n is (A) (B) (C) (D) 120–130 ml/minut e 325–350 ml/minute 480–52 ml/minute 560–830 ml/minute 320. The r te of f tty cid oxid tion is incre sed olipids (B) Glycolipids (D) All of these
y (A) Phospholipids (C) Amin
312. Filtr tion fr ction c n e c lcul ted from (A) St nd rd ure cle r nce nd PSP excretion (B) M ximum ure cle r nce nd PSP excretion (C) M ximum ure cle r nce nd PAH cle r nce (D) Inulin cle r nce nd PAH cle r nce 313. Norm l filtr tion fr ction is out (A) 0.2 (C) 0.6 (A) (B) (C) (D) (B) 0.4 (D) 0.8 321. Lecithin cont ins nitrogenous (B) Choline (D) All of these 322. Lecithins cont in ) None of these
se n med
n uns tur ted f tty
cid
s (A) Eth nol mine (C) Inositol t position: (A) α (C) β (B) α
nd β (D
314. Filtr tion fr ction is incre sed in Acute glomerulonephritis Chronic glomer ulonephritis Hypertension Hypotension 323. Lecithins re solu le in ordin ry solvents except (A) Benzene (C) Methyl cohol (B) Ethyl lcohol (D) Acetone
l
324. Lecithins com ine with protein to form (A) Phosphoprotein (C) Lipoprotein ( B) Mucoprotein (D) Glycoprotein 315. Among the following, test of Glomerul r function is (A) (B) (C) (D) Ure cle r nce PSP excretion test PAH cle r nce Hippuric cid excretion test 325. Inste d of ester link pl sm logens possess C) γ (B) β (D) None of these
n other link in position: (A) α (
FATS AND FATTY ACID METABOLISM 326. The alkyl radical in plasmalogen is an alcohol: (A) Saturated (C) Both (A) and (B) (B) Unsaturated (D) None of these 93 336. Lipoprotiens may e identified more accurately y means of (A) (B) (C) (D) Electrophoresis Ultra centrifugation Centrifugation Immunoelectrophoresis 327. The concentration of sphingomyelins are increased in (A) (B) (C) (D) Gauche r’s disease Fa ry’s disease Fa rile disease Niemann Pick disease 337. Very low density lipoproteins are also known as (A) β lipoproteins (C) α-lipopr
oteins (B) Pre β--lipoproteins (D) None of these 328. Sphingomyelins cont in complex mino lcohol n med s (A) Serine (C) Sphi ngosine (A) 1 (C) 4 (A) Sphingosine (C) Both (A) nd (B) (A) Sphingolipids (C) A minolipids (B) Lysolecithin (D) Glycol (B) 3 (D) 5 (B) Iso-sphingosine (D) None of these (B) Sulpholipids (D) Glycolipids 338. The protein moiety of lipoprotein is known in (B) Pre-protein (D) Pseudoprotein 329. The types of sphingomyelins
s (A) Apoprotein (C) Post-prote
re
339. The β-lipoprotein fr ction incre ses in severe (A) Di etes Mellitus (B) Urem i (C) Nephritis (D) Muscul r dystrophy 340. ∆ 9 indic tes dou le ond etween c r on toms of the f tty cids: (A) 8 nd 9 (C) 9 nd 11 (B) 9 nd 10 (D) 9 nd 12 330. Glycolipids cont in 331. Cere rosides m y
n
lso
mino
lcohol:
e cl ssified
s
332. G ucher ’s dise se is ch r cterized speci lly y the incre se in (A) (B) (C) (D) Lignoceric cid Nervonic cid Cere omic cid Hydroxynervonic cid (B) Liver (D) Muscle 341. The num er of c r on (B) 8 (D) 12 342. Ar chidonic 5 333. G ngliosides
toms in dec noic
cid present in
cid cont ins the num er of dou le
utter: (A) 6 (C) 10
onds: (A) 2 (C) 4 (B) 3 (D)
re the glycolipids occurring in (A) Br in (C) Kidney
343. The prost gl ndins re synthesized from (A) Ar chidonic cid (B) Oleic cid (C) Linoleic cid (D) Linolenic cid 344. The Iodine num er of essenti l f tty cids of veget le oils: (A) High (C) Very low 345. Cholesterol is (A) Anim l sterol (C) 5 methyl groups (B) M.F. C27 H46O (D) All of these (B) Very high (D) Low 334. Lipoprotein present in cell mem r ne is y n ture: (A) Hydrophilic (C) Both (A) nd (B) (B) Hydropho ic (D) None of these 335. The density of lipoproteins incre ses s the protein content (A) (B) (C) (D ) Incre ses Decre ses Highly decre ses Slightly nd promptly decre ses
94 346. W xes cont in higher Cetyl
lcohols n med
s (A) Methyl (C) Phytyl (B) Ethyl (D)
MCQs IN BIOCHEMISTRY 356. C r oxyl tion of cetyl —CoA to m lonyl — CoA t kes pl ce in presence of (A) FA D+ (C) NAD+ (A) —SH group (C) —COOH group (B) Biotin (D) NADP+ (B) —NH2 group (D) —CH2OH group 347. Lie erm n-Burch rd re ction is performed to detect (A) Cholesterol (C) F tt y cid (B) Glycerol (D) Vit min D 357. M lonyl-CoA re cts with the centr l 348. Lipose present in the stom ch c nnot hydrolyze f ts owing to (A) Alk linity (C) High cidity (A) (C) α -oxid tion ω -oxidation 358. Fatty acid synthesis takes place in the presence of the coenzyme: (A) NAD+ (C) NADP+ (B) Reduced NAD (D) Reduced NADP (B) Acidity (D) Neutrality (B) β -oxidation (D) All of these 349. Fatty acids are oxidized
y
359. Fatty acids are activated to acyl CoA ) CoA (B) NADP+ (D) FAD+
y the enzyme thiokinase: (A) NAD+ (C
350. The fatty acids containing even num er and odd num er of car on atoms as e ll as the unsaturated fatty acids are oxidized y (A) α-oxid tion (C) ω-oxidation (B ) β-oxidation (D) All of these 360. Phospholipids help the oxidation of (A) Glycerol (B) Fatty acids (C) Glycer ophosphates(D) None of these 361. The desaturation and chain elongation system o f polyunsaturated fatty acids are greatly diminished in the a sence of (A) Insul in (C) Epinephrine (B) Glycagon (D) Thyroxine 351. Long chain fatty acids are first activated to acyl CoA in the (A) Cytosol ( C) Ri osomes (B) Mitochodria (D) Microsome 352. Long chain acyl CoA penetrates mitochondria in the presence of (A) Palmitat e (C) Sor itol (B) Carnitine (D) DNP 362. Prostaglandins are li erated in the circulation y the stimulation of (A) ( B) (C) (D) Anterior pituitary glands Posterior pituitary glands Adrenal gland Th yroid gland 353. Acyl-CoA dehydrogenase converts Acyl CoA to α - β uns tur ted nce of the coenzyme: (A) NAD+ (C) ATP (B) NADP+ (D) FAD 363. Prost gl ndins h ve common structure sed on prost noic ins c r on toms: (A) 12 (C) 18 (B) 16 (D) 20
cyl-CoA in prese
cid which cont
354. For the ctiv tion of long ch in f tty cids the enzyme thiokin se requires the cof ctor: (A) Mg ++ (C) Mn++ (B) C ++ (D) K + 364. The c r on ch ins of prost noic cid re onded t the middle of the ch in y (A) 5-mem ered ring (B) 6-mem ered ring (C) 8-mem ered ring (D) None of the se 365. All ctive prost gl ndins h ve tle st one dou le ond etween positions : (A) 7 nd 8 (C) 11 nd 12 (B) 9 nd 10 (D) 13 nd 14
355. ω -oxidation takes place y the hydroxylase in microsomes involving (A) Cytoc hrome (B) Cytochrome c (C) Cytochrome p-4500(D) Cytochrome a3
FATS AND FATTY ACID METABOLISM 366. The enzyme systems for lengthening and shortening for saturating and desatu rating of fatty acids occur in (A) Intestine (C) Kidney (B) Muscle (D) Liver 95 374. Which of the follo ing is not an unsaturated fatty acid? (A) Oleic acid (C) Linaoleic acid (B) Stearic acid (D) Palmitic acid 367. Which of the follo ing are classified as essential fatty acids? (A) Arachid onic acid (B) Oleic acid (C) Acetic acid (D) Butyric acid 368. Prostaglandins ar e synthesized in the ody from (A) Myristic acid (C) Stearic acid (B) Arachidoni c acid (D) Lignoceric acid 375. All the follo ing are functions of prostaglandins except (A) Lo ering of B. P (B) Introduction of la our (C) Anti inflammatory (D) Prevention of myocardial infraction 376. Calorific value of lipids per gm is (A) 4 Kcal (C) 9 Kcal (A) Li gnoceric acid (C) Nervonic acid (B) 8 Kcal (D) None of these (B) Cere romic acid (D) Hydroxynervonic acid 369. All the follo ing saturated fatty acids are present in uffer except (A) Bu tyric acid (C) Caproic acid (B) Capryllic acid (D) Capric acid 377. Fatty acid present in kerotin is 378. All the follo ing are ketones except (A) Xylulose (C) Erythrose 379. Saponi fication: (A) (B) (C) (D) Hydrolysis of fats y alkali Hydrolysis of glycerol y liposes Esterification Reduction (B) Ri olose (D) Fructose 370. Biological functions of lipids include (A) Source of energy (B) Insulating material (C) Maintenance of cellular integrity (D) All of these 371. Saponificat ion num er is (A) mg of KOH required to saponify one gm of fat or oil (B) mg of KOH required to neutralize free fatty acids of one gms of fat (C) mg of KOH requ ired to neutralize the acetic acid o tained y saponification of one gm of fat a fter it has een acetylated (D) None of these 372. Lipids have the follo ing pro perties: (A) Insolu le in ater and solu le in fat solvent (B) High energy conte nt (C) Structural component of cell mem rane (D) All of these 373. Car ohydrate moiety in cere rosides is (A) Glucose (C) Galactose (B) Sucrose (D) Maltose 380. Num er of ml of 0.1 N KOH required to neutralize fatty acids from 5 gms of fat: (A) (B) (C) (D) Iodine num er Polenske num er Reichert-Miessl num er None o f these 381. Hydrated density of HD lipoproteins is (A) (B) (C) (D) 0.94 gm/ml 0.94–1.006 gm/ml 1.006–1.063 gm/ml 1.063–1.21 gm/ml 382. Saponification num er indicates (A) (B) (C) (D) Unsaturation in fat Average M.W of fatty acid Acetyl num er Acid num er
96 383. Acrolein Test is positive for (A) Glycerol (C) Car ohydrates (A) (B) (C) (D ) (B) Prostaglandins (D) Proteins MCQs IN BIOCHEMISTRY 392. The smell of fat turned rancid is due to (A) Presence of vit E (C) Phenols (B) Presence of quinones (D) Volatile fatty acids 384. Iodine num er denotes Degree of unsaturation Saponification num er Acid num er Acetyl num er (B) Car ohydrates (D) Nucleic acids acids + Phosphoric acid + acids + Phosphoric acid + acids + Phosphoric acid + acids + Phosphoric acid + 393. Phospholipids are important cell mem rane components ecause (A) They have glycerol (B) They can form ilayers in ater (C) They have oth polar and non po lar potions (D) They com ine covalently ith proteins 394. Which one of the foll o ing is not a phospholipid? (A) Lecithin (C) Lysolecithin (B) Plasmalogen (D) G angliosides 385. Maximum energy produced y (A) Fats (C) Proteins (A) Glycerol + Fatty Choli ne (B) Glycerol + Fatty Ethanolamine (C) Glycerol + Fatty Serine (D) Glycerol + Fatty Beaine 386. Lecithins are composed of 395. A fatty acid hich is not synthesized in human ody and has to e supplied in the diet: (A) Palmitic acid (C) Linoleic acid (B) Oleic acid (D) Stearic acid 396. In cephalin, choline is replaced (D) Sphingosine
y (A) Serine (C) Betaine (B) Ethanolamine
387. Sphingomyelins are composed of fatty acids, phosphoric acid and (A) (B) (C) (D) Sphingosine and choline Glycerol and sphingosine Glycerol and Serine Glycer ol and Choline 397. The triacyl glycerol present in plasma lipoproteins are hydrolyzed inqual lipase (C) Colipase (B) Pancreatic lipase (D) Lipoprotein lipase
y (A) L
388. Depot fats of mammalian cells comprise mostly of (A) Cholesterol (C) Triacy l glycerol (B) Cholesterol esters (D) Phospholipids 398. Amphiphatic lipids are (A) Hydrophilic (C) Both (A) and (B) (B) Hydropho ic (D) Lipophilic 389. When choline of lecithine is replaced y ethanolamine the product is (A) Sp hingomyelin (C) Plasmalogens (B) Cephalin (D) Lysolecithine 399. Which of the follo ing is not essential fatty acid? (A) Oleic acid (B) Lino leic acid (C) Arachidonic acid (D) Linolenic acid 400. The calorific value of li pid is (A) 4.0 Kcal/gm (C) 9.0 Kcal/gm (B) 6.0 Kcal/gm (D) 15 Kcal/gm 390. Which of the follo ing is a hydroxy fatty acid? (A) Oleic acid (C) Caproic acid (B) Ricinoleic acid (D) Stearic acid 391. Acrolein test is ans ered ) Sphingol
y (A) Cholesterol (C) Glycosides (B) Glycerol (D
401. Rancidity of utter is prevented y the addition of (A) Vitamin D (B) Tocop herols (C) Presence of priotin (D) Presence of ‘Cu’
FATS AND FATTY ACID METABOLISM 402. Sphingomyelins on hydrolysis yields (A) Glycerol, fatty acids, phosphoric a cid and choline (B) Glycerol, sphingosine, choline and fatty acids (C) Sphingosi ne, phosphoric acid, Glycerol and inositol (D) Sphingosine, fatty acids, phospho ric acid and choline 403. Inherited deficiency of enzyme cere rosidase produces (A) (B) (C) (D) Fa ry’s disease Niemann pick disease Gaucher’s disease Tay-sach’s dise ase 97 410. Cholesterol is the precursor for the iosynthesis of (A) fatty acid (C) e acids (B) prostaglandins (D) sphingmyelin
il
411. Which of the follo ing condition is characterized y ketonuria ut ithout glycosuria? (A) (B) (C) (D) Dia etes mellitus Dia etes insipidus Prolonged starv ation Addison’s disease (B) Liver (D) Intestines 412. Ketone
odies are formed in (A) Kidney (C) Heart
404. Phosphatidic acid on hydrolysis yields (A) Glycerol, fatty acids, phosphori c acid, choline (B) Glycerol, fatty acids, phosphoric acid (C) Glycerol, fatty a cids, phosphoric acid, Glucose (D) Sphingol, fatty acids, phosphoric acid 405. T he maximum num er of dou le onds present in essential fatty acid is (A) 1 (C) 3 (B) 2 (D) 4 413. Changes in serum high density lipoproteins (HDL) are more truly reflected y those of (A) HDL-1 (C) HDL-3 (A) (B) (C) (D) (B) HDL-2 (D) HDLC 414. Mitochondrial lipogenesis requires e NADPH
icar onate
iotin acetyl CoA car oxylas
406. Cere rosides are composed of (A) Sphingosine, fatty sphoric acid (B) Sphingosine, fatty acids, galactose (C) galactose (D) Glycerol, fatty acids, galactose, sphingol nd β-OH utyric acid are formed as (A) Kidneys (C) Liver (B) Heart (D) Intestine (B) Leucine (D) Methionine
acids, glycerol and pho Glycerol, fatty acids, 407. Acetoacetic acid a (A) Lysine (C) Tryptophan
415. Fatty acids having chain length of 10 car on atoms enter the (A) Portal cir uclation (B) Lacteals (C) Systemic circulation (D) Colon 416. A solu le system f or synthesis of fatty acids have een isolated from avian liver, required for th e formation of long chain fatty acids y this system is (A) ATP (C) NADPH (B) Ac etyl CoA (D) All of these 408. Which amino acid is a lipotropic factor? 417. Most animal tissues contain apprecia le amounts of lipid, hen in the form of depot fat it consists largely of (A) Cholesterol ester (C) Chylomicrons (A) O leic (C) Linoleic (B) Phosphatides (D) Triacylglycerol (B) Palmitic (D) Stearic 409. The class of lipoproteins having a eneficial effect in atherosclerosis is (A) (B) (C) (D) Lo density of lipoproteins very lo density lipoproteins High d ensity lipoproteins Chylomicrons 418. A fatty acid not synthesized in man is
98 419. The ‘free fatty acids’ (FFA) of plasma: (A) (B) (C) (D) meta olically inert mai nly ound to β-lipoproteins stored in the fat mainly ound to serum al umin MCQs IN BIOCHEMISTRY 425. In the type II (a) hyper lipoproteinemia there is increase in (A) Chylomicr on ond (B) β (C) Pre eta (D) α 426. Norm l f t content of liver is out _______ g ms %. (A) 5 (C) 10 (B) 8 (D) 15 420. Adipose tissue which is store house for tri cyl glycerol synthesis the s me using (A) The glycerol rele sed y hydrolysis of tri cyl glycerol (B) The gly cerol-3-phosph te o t ined in the met olism of glucose (C) 2-phosphoglycer te ( D) 3-phosphoglycer te 421. Incre se in lood of this cl ss of lipoproteins is e nefici l to w rd off coron ry he rt dise se: (A) HDL (C) VLDL (B) LDL (D) IDL 427. O esity is (D) Proteins
ccumul tion of _______ in the
428. The first lipoprotein to scent VLDL (D) IDL
e secreted
ody. (A) W ter (C) F t (B) N Cl
y the liver is (A) VLDL (C) LDL (B) n
422. In the extr mitochondri l synthesis of f tty cids, CO2 is utilized (A) To keep the system n ero ic nd prevent regener tion of cetyl CoA (B) In the con version of m lonyl to CoA hydroxy utyryl CoA (C) In the conversion of cetyl CoA to m lonyl CoA (D) In the form tion of cetyl CoA from 1 c r on intermedi tes 4 23. Current concepts concerning the intestin l sorption of tri cylglycerols r e th t (A) They must e completely hydrolysed efore the constituent f tty cids c n e sor ed (B) They re hydrolysed p rti lly nd the m teri l sor ed con sists of free f tty cids, mono nd di cyl glycerols nd unch nged tri cyl glyce rols (C) F tty cids with less th n 10 c r on toms re sor ed out equ lly v i lymph nd vi port l lood (D) In the sence of ile the hydrolysis of tri c yl glycerols is sor ed 424. M in met olic end product of cholesterol: (A) Cop rosterol (C) Bile cid (B) 5-pregnenolone (D) Glycine 429. This lipoprotein removes cholesterol from the (D) Chylomicrons
ody (A) HDL (C) IDL (B) VLDL
430. When the stired tri cylglycerol is lipolysed in the dipose tissue lood le vels of _____ incre sed. (A) (B) (C) (D) FFA only Glycerol only Free f tty cids (FFA) nd Glycerol Tri cyl glycero 431. All long ch in f tty cids with even num er of c r on toms re oxidized to pool of _________ y β -oxid tion. (A) CO2 (C) Acetic cid (B) Propionic cid ( D) Acetyl CoA 432. The level of free f tty cose (B) C ffeine (D) Ni cin
cids in pl sm
is incre sed
y (A) Insulin (C) Glu
433. Cholesterol is excreted s (D) Te rs
s such into ________. (A) Urine (C) Bile (B) F ece
FATS AND FATTY ACID METABOLISM 434. LCAT is (A) L ctose choline l mine tr nsferse (B) Lecithin cholesterol cy l tr nsfer se (C) Lecithin c rnitine cyl tr nsfer se (D) L nole te c r moyl c yl tr nsfer se 435. Cholesterol molecule h s _______ c r on toms. (A) 27 (C) 15 (B) 21 (D) 12 99 442. Cholesterol circul tes in lood stre m chiefly s (A) (B) (C) (D) Free chol esterol Ester cholesterol Low density lipoproteins Low density lipoproteins nd high density lipoproteins 443. Wh t is the su cellul r site for the βoxid tion of f tty (C) Lysosome (B) Mitochondri (D) Cytosol
cids? (A) Nucleus
436. A hydroc r on formed in cholesterol synthesis is (A) Mev lon te (C) Squ len e (B) HMG CoA (D) Zymosterol 444. A diet cont ining this f t is helpful in lowering the lood cholesterol lev el. (A) Uns tur ted (B) S tur ted (C) Vit min enriched (D) Refined 445. Phosphol ip se A2 is n enzyme which removes f tty cid residue from lecithin to form ( A) (B) (C) (D) Lecithin fr gments Phosphotidic cid Glyceryl phosph te Lysolecit hin 437. While citr te is converted to isocitr te in the mitochondri , it is convert ed to _______ in the cytosol. (A) Acetyl CoA + ox lo cet te (B) Acetyl CoA + m l onyl CoA (C) Acetyl CoA + Pyruv te (D) Acetyl CoA + ceto cetyl CoA 438. Avidin is ntigonistic to (A) Ni cin (C) Biotin (B) PABA (D) P ntothenic cid 446. P ncre tic lipose is n enzyme which hydrolyzes f cts. It peptid se (C) c r ohydr tes (B) hydrol se (D) dehydrogen se 439. CTP is required for the synthesis of (A) F tty Proteins (D) Cholesterol
cts
s
/ n (A)
cids (C) Phospholipids (B)
447. This interferes with cholesterol sorption (A) (B) (C) (D) Lipoprotein lip se Cre tin se 7-dehydrocholesterol β-sitosterol 440. Lysolecithin is formed from lecithin y the ction of (A) Phospholip se A1 (B) Phospholip se A2 (C) Phospholip se C (D) Phospholip se D 441. F tty cids c n not e converted into c r ohydr tes in the ody, s the following re ction is not possi le: (A) Conversion of glucose-6-phosph te into glucose (B) Fructose 1, 6 diphosph te to fructose-6phosph te (C) Tr nsform tion of cetyl CoA to pyruv te (D) Form tion of cetyl CoA from f tty cids 448. The c r on ch in of f tty cids is shortened y 2 c r on toms t time. T his involves successive re ctions c t lysed y 4-enzymes. These ct the followin g order: (A) Acetyl CoA dehydrogen se, -OH cyl CoA dehydrogen se, enoyl hydr se, thiolose (B) Acyl CoA dehydrogen se, thiol se, enoyl hydr se, β-OH cyl CoA dehyd rogen se (C) Acyl CoA dehydrogen se, thiolose, enoyl hydr se, β-OH cyl CoA dehydr ogen se (D) Enoyl hydr se, β-OH cyl CoA dehydrogen se, cyl CoA dehydrogen se, th iolose,
100 449. Acyl c rrier protein is involved in the synthesis of (A) (B) (C) (D) protei n glycogen f tty cid outside the mitochondri f tty cid in the mitochondri MCQs IN BIOCHEMISTRY 457. Very low density lipoproteins re rel tively rich in (A) Cholesterol (C) Fr ee f tty cids (A) Liver (C) Adipose tissue (B) Tri cyl glycerol (D) Phospholipi ds (B) P ncre s (D) Br in 458. Neutr l f t is stored in 450. 1 molecule of p lmitic cid on tot l oxid tion to CO2 will yield molecules of ATP ( s high energy onds): (A) 129 (C) 83 (B) 154 (D) 25 459. A p thw y th t requires NADPH s cof ctor is (A) F tty cid oxid tion (B) Extr mitochondri l denovo f tty cid synthesis (C) Ketone odies form tion (D) Glycogenesis 460. The ‘Committed step’ in the iosynthesis of cholesterol from cet yl CoA is (A) Form tion of ceto cetyl CoA from cetyl CoA (B) Form tion of mev lon te from HMG CoA (C) Form tion of HMG CoA from cetyl CoA nd ceto cetyl CoA (D) Form tion of squ lene y squ lene synthet se 461. In β-Oxid tion of f tty ci ds, which of the following re utilized s coenzymes? (A) (B) (C) (D) NAD+ nd N ADP+ FADH2 nd NADH + H+ FAD nd FMN FAD nd NAD+ 451. HMG CoA is formed in the met olism of (A) (B) (C) (D) Cholesterol, ketones nd leucine Cholesterol, f tty cid nd Leucine Lysine, Lecuine nd Isoleucine Ketones, Leucine nd Lysine 452. NADPH is produced when this enzyme cts (A) (B) (C) (D) Pyruv te dehydrogen se M lic enzyme Succin te dehydrogen se M l te dehydrogen se 453. As result of e ch oxid tion long ch in f tty cid is cle ved to give (A ) (B) (C) (D) An cid with 3-c r on less nd propionyl CoA An cid with 2-c r on less nd cetyl CoA An cid with 2-c r on less nd cetyl CoA An cid with 4 -c r on nd utyryl CoA 454. Liposomes re (A) Lipid il yered (B) W ter in the middle (C) C rriers of d rugs (D) All of these 455. Long ch in f tty cyl CoA esters re tr nsported cro ss the mitochondri l mem r ne y (A) cAMP (C) C rnitine (B) Prost gl ndin (D) Ch oline 462. The most import nt source of reducing equiv lents for FA synthesis on the l iver is (A) (B) (C) (D) Glycolysis HMP-Shunt TCA cycle Uronic cid p thw y 463. All of the following tissue re c p le of using ketone r in (C) R.B.C. (B) Ren l cortex (D) C rdi c muscle
odies except (A) B
456. The cetyl CoA formed on β-oxid tion of ll long ch in f tty cids is met ol ized under norm l circumst nces to (A) CO2 nd w ter (C) F tty cids (B) Cholest erol (D) Ketone odies 464. The m jor source of cholesterol in IDL (C) HDL (B) LDL (D) Chylomicrons
rteri l smooth muscle cells is from (A)
FATS AND FATTY ACID METABOLISM 465. Ketone odies re synthesized from f tty cid oxid tion products y which o f the following org ns? (A) Liver (C) Kidney (B) Skelet l muscles (D) Br in 101 472. In synthesis of Triglyceride from α-Glycero phosph te nd cetyl CoA, the fir st intermedi te formed is (A) β-di cyl glycerol (B) Acyl c rnitine (C) Mono cyl gl ycerol (D) Phosph tidic cid 473. During e ch cycle of β-oxid tion of f tty cid, ll the following compounds re gener ted except (A) NADH (C) FAD (B) H2O (D) Ac yl CoA 466. Ch in elong tion of f tty cids occurring in m mm li n liver t kes pl ce in which of the following su cellul r fr ctions of the cell? (A) Nucleus (C) Lysos omes (B) Ri osomes (D) Microsomes 467. Which of the following cof ctors or their deriv tives must e present for t he conversion of cetyl CoA to m lonyl CoA extr mitochondri l f tty cid synthes is? (A) Biotin (C) FMN (B) FAD (D) ACP 474. The energy yield from complete oxid tion of products gener ted y second re ction cycle of β-oxid tion of p lmitoyl CoA will e (A) 5 ATP (C) 17 ATP (B) 12 A TP (D) 34 ATP 468. Which of the following st tement reg rding β-oxid tion is true? (A) (B) (C) ( D) Requires β-keto cyl CoA s su str te Forms CoA thioesters Requires GTP for it s ctivity Yields cetyl CoA s product 475. β -Oxid tion of odd-c r on f tty cid ch in produces (A) Succinyl CoA (C) Ace tyl CoA (B) Propionyl CoA (D) M lonyl CoA 476. Brown dipose tissue is ch r cterized y which of the following? (A) Presen t in l rge qu ntities in dult hum ns (B) Mitochondri l content higher th n whit e dipose tissue (C) Oxid tion nd phosphoryl tion re tightly coupled (D) A sen t in hi ern ting nim ls 477. Ketosis in p rtly scri ed to (A) (B) (C) (D) Over production nd Glucose Under production of Glucose Incre sed c r ohydr te utili z tion Incre sed f t utiliz tion 469. All st tements reg rding 3-OH-3 methyl glut ryl CoA re true except (A) (B) (C) (D) It is formed in the cytopl sm Required in ketogenesis Involved in synth esis of F tty cid An intermedi te in cholesterol iosynthesis 470. Which of the following lipoproteins would contri ute to me surement of pl sm cholesterol in norm l individu l following 12 hr f st? (A) (B) (C) (D) Chylomicrons VLDL Both VLDL nd LDL LDL 478. The free f tty cids in lood re (A) (B) (C) (D) Stored in f t depots M in ly ound to β-lipoproteins M inly ound to serum l umin Met olic lly most in cti ve 471. All the following st tements reg rding ketone odies re true except (A) (B ) (C) (D) They m y result from st rv tion They re formed in kidneys They includ e ceto cetic cid nd cetone They m y e excreted in urine 479. C rnitine is synthesized from (A) Lysine (C) Choline (B) Serine (D) Arginin e
102 480. A met olite which is common to p thw ys of cholesterol iosynthesis from cetyl-CoA nd cholec lciferol form tion from cholesterol is (A) (B) (C) (D) Zymo sterol Lumisterol Ergosterol 7 Dehydrocholesterol MCQs IN BIOCHEMISTRY the correct ordering of these p rticles from the lowest to the gre test density? (A) (B) (C) (D) LDL, IDL, VLDL, Chylomicron Chylomicron, VLDL, IDL, LDL VLDL, I DL, LDL, Chylomicron Chylomicron, IDL, VLDL, LDL 488. A compound norm lly used to conjug te ic cid (B) Glycine (D) F tty cid
ile
cids is (A) Serine (C) Glucoron
481. Acetyl CoA required for extr mitochondri l f tty cid synthesis is produce d y (A) (B) (C) (D) Pyruv te dehydrogen se complex Citr te ly se Thiol se C rni tine- cyl tr nsfer se 489. Which of the following lipoproteins would contri ute to me surement of pl sm cholesterol in norm l person following 12 hr f st? (A) (B) (C) (D) High density lipoprotiens Low density lipoproteins Chylomicron Chylomicron remn nts 482. Biosynthesis of Triglyceride nd Lecithine oth require n intermedi te: (A ) (B) (C) (D) Mono cyl glycerol phosph te Phosph tidic cid Phosph tidyl eth nol mine Phosph tidyl cytidyl te 483. The r ge limiting step cholesterol iosynthesis is (A) (B) (C) (D) Squ lene synthet se Mev lon te kin se HMG CoA synthet se HMG CoA reduct se 490. Which of the following products of tri cylglycerol re kdown nd su sequent β -Oxid tion m y undergo gluconeogenesis? (A) Acetyl CoA (B) Porpionyl CoA (C) Al l ketone odies (D) Some mino cids 491. Which of the following regul tes lipol ysis in dipocytes? (A) Activ tion of f tty cid synthesis medi ted y CAMP (B) Glycerol phosphoryl tion to prevent futile esterific tion of f tty cids (C) Act iv tion of triglyceride lip se s result of hormone stimul ted incre ses in CA MP levels (D) Activ tion of CAMP production y Insulin 492. Which one of the fol lowing compounds is key intermedi te in the synthesis of oth tri cyl glycerol s nd phospholipids? (A) CDP Choline (C) Tri cyl glyceride (B) Phosph tid se (D) Phosph tidyl serine 484. All the following re constituents of g nglioside molecule except (A) Glyce rol (C) Hexose sug r (B) Si lic cid (D) Sphingosine 485. An lcoholic mine residue is present in which of the following lipids? (A) Phosph tidic cid (B) Cholesterol (C) Sphingomyelin (D) G nglioside 486. Sphing osine is the ck one of ll the following except (A) Cere roside (C) Sphingomye lin (B) Cer mide (D) Lecithine 487. Chylomicron, intermedi te density lipoproteins (IDL), low density lipoprote ins (LDL) nd very low density lipoproteins (VLDL) ll re serum lipoproteins. W h t is 493. During e ch cycle of on going f tty cid oxid tion, ll the following compo unds re gener ted except (A) H2O (C) F tty cyl CoA (B) Acetyl CoA (D) NADH
FATS AND FATTY ACID METABOLISM 494. All the following st tements descri ing lipids re true except (A) They usu lly ssoci te y cov lent inter ctions (B) They re structur lly components of mem r nes (C) They re n intr cellul r energy source (D) They re poorly solu l e in H2O 495. All the following st tements correctly descri e ketone odies exce pt (A) They m y result from st rv tion (B) They re present t high levels in un controlled di etes (C) They include—OH β- utyr te nd cetone (D) They re utilized y the liver during long term st rv tion 496. Which of the following fe tures i s predicted y the Nicolson–Singer fluid mos ic model of iologic l mem r nes? (A) Mem r ne lipids do not diffuse l ter lly (B) Mem r ne lipid is prim rily in m onol yer form (C) Mem r ne lipids freely flip-flop (D) Mem r ne proteins m y dif fuse l ter lly 497. Oxid tive degr d tion of cetyl CoA in the citric cid cycle gives net yield of ll the following except (A) FADH2 (C) 2 ATP (B) 3 NADH (D ) 2CO2 103 500. Mitochondri l α-ketoglut r te dehydrogen se complex requires g to function except (A) CoA (C) NAD+ (B) FAD (D) NADP+
ll the followin
501. E ch of the following c n e n intermedi te in the synthesis of phosph tid yl choline except (A) (B) (C) (D) Phosph tidyl inositol CDP-choline Phosph tidyl eth nol mine Di cylglycerol (B) C r oxyl groups (D) Uns tur tion 502. High iodine v lue of ps
lipid indic tes (A) Polymeriz tion (C) Hydroxyl grou
503. Cholesterol, ile s lts, vit min D nd sex hormones Phospholipids (B) Glycolipids (D) Isoprenoid lipids 504. W ter solu le molecul r ggreg tes of lipids phingol (B) Colloids (D) Mucin
re (A) Mucolipids (C)
re known
s (A) Micelle (C) S
505. Hypoglycemi depresses insulin secretion nd thus incre ses the r te of (A) Hydrolysis (B) Reduction (C) Gluconeogenesis (D) Respir tory cidosis 506. The process of re kdown of glycogen to glucose in the liver nd pyruv te nd l c te in the muscle is known s (A) Glyogenesis (B) Glycogenolysis (C) Gluconeogenesi s (D) Cellul r degr d tion 507. Across mem r ne phospholipids ct s c rrier o f (A) (B) (C) (D) Org nic compounds Inorg nic ions Nucleic cids Food m teri ls 498. All the following correctly descri e the intermedi te 3-OH-3-methyl glut ry l CoA except (A) It is gener ted enzym tic lly in the mitochondri l m trix (B) I t is formed in the cytopl sm (C) It inhi its the first step in cholesterol synth esis (D) It is involved in the synthesis of ketone odies 499. Intermedi te in t he denovo synthesis of tri cyl glycerols include ll the following except (A) (B ) (C) (D) F tty cyl CoA CDP di cyl glycerol Glycerol-3-phosph te Lysophosph tid ic cid 508. Osteom l ci c n e prevented n: (A) A (C) C (B) B (D) D
y the
dministr tion of c lcium
nd
vit mi
104 509. Milk sug r is known MCQs IN BIOCHEMISTRY 519. The m jority of hylomicrone (D) LDL
s (A) Fructose (C) Sucrose (B) Glucose (D) L ctose
sor ed f t
ppe rs in the forms of (A) HDL (C) VLDL (B) C
510. The Instrinisic F ctor (HCl nd mucoproteins) present in the g stric juice help in the sorption of (A) Vit min B2 (C) Folic cid (A) 2.5–4 (C) 4 to 5 512. Bile is produced y (A) Liver (C) P ncre s (A) Retin l (C) C rotene (B) G ll- l dder (D) Intestine (B) Retinol (D) Repsin (B) Tocopherols (D) Vitm in B12 (B) 3. 5–5 (D) 5–7 520. D ily output of ure in gr ms is (A) 10 to 20 (C) 20 to 30 521. Uremi occu rs in (A) Cirrohsis of liver (B) Nephritis (C) Di etes mellitus (D) Coron ry th rom osis 522. C r oxyhemoglo in is formed y (A) CO (C) HCO3 (B) CO2 (D) HCN (B) 15 to 25 (D) 35 to 45 511. Lip se c n
ct only
t pH:
513. Non-protein p rt of rhodopsin is 523. Methemoglo in is formed s result of the oxid tion of h emoglo in tion gent: (A) Oxygen of Air (C) K4Fe(CN)6 (B) H2O2 (D) KMnO4 514. A p thw y th t requires NADPH ndri l folic cid synthesis Ketone
y oxid
s cof ctor is (A) (B) (C) (D) Extr mitocho ody form tion Glycogenesis Gluconeogenesis
524. Methemoglo in c n e reduced to h emoglo in ydrogen Vit min C Glut thione Cre tinine
y (A) (B) (C) (D) Remov l of h
515. LCAT ctivity is ssoci ted with which of the lipo-protein complex? (A) VLD L (C) IDL (B) Chylomicrones (D) HDL 525. F ts
re solids
t (A) 10°C (C) 30°C (B) 20°C (D) 40°C
516. In β− β−oxidation of fatty acids which of the followin are utilized as co enzymes? (A) (B) (C) (D) NAD+ and NADP+ FAD H2 and NADH + H+ FAD and FMN FAD and NAD+ 526. Esters of fatty acids with hi her alcohol other than (A) Oils (C) Waxes (B) Polyesters (D) Terpenoids
lycerol are called as
527. The main physiolo ical buffer in the blood is (A) (B) (C) (D) Haemo lobin b uffer Acetate Phosphate Bicarbonate 517. The lipoprotein with the fastest electrophoretic mobility and lowest TG con tent are (A) VLDL (C) HDL (A) Atherosclerosis (C) Nepritis (B) LDL (D) Chylomicr ones (B) Diabetes mellitus (D) Oedema 518. The essential fatty acids retard 528. All of the followin substances have been used to estimate GFR except (A) I nulin (C) Phenol red (B) Creatinine (D) Mannitol
FATS AND FATTY ACID METABOLISM 529. Relationship between GFR and seum creatinine concentration is (A) Non exist ent (C) Direct (B) Inverse (D) Indirect 105 538. For the activity of amylase which of the followin ? (A) HCO3 (C) K + (B) Na + (D) Cl
is required as co factor
530. Urine turbidity may be caused by any of the followin (C) RBC (A) (B) (C) (D) (B) Protein (D) WBC
except (A) Phosphates
539. Which of the followin hormone increases the absorption of lucose from G.I .T? (A) Insulin (C) Gluca on (A) Carbohydrates (C) Lipids (A) Mitochondrion (C) Cytosol of cell (B) Throid hormones (D) FSH (B) Fats (D) Both (B) and (C) (B) Er ythrocytes (D) R.E. cells 531. Urine specific ravity of 1.054 indicates Excellent renal function Inapprop riate secretion of ADH Extreme dehydration Presence of lucose or protein 540. Predominant form of stora e: 541. De radations of Hb takes place in 532. In hemolytic jaundice, the urinary bilirubin is (A) (B) (C) (D) Normal Abse nt More than normal Small amount is present 542. Biluveridin is converted to bilirubin by the process of (A) Oxidation (C) C onju ation (A) α-Amyl se (C) γ Amylase (B) Reduction (D) Decarboxylation (B) β-Amylae (D) All of these 533. In o structive jaundice, urinary sed Present Present in small amount
iliru in is (A) (B) (C) (D) A sent Increa
543. Amylase present in saliva is 544. Phospholipids are important cell mem rane components since (A) (B) (C) (D) They have glycerol Form ilayers in ater Have polar and non-polar portions Com ine covalently ith proteins 534. In hemolytic jaundice, iliru in in urine is (A) (B) (C) (D) Usually a sent Usually present Increased very much Very lo 545. Which of the follo ing is not a phospholipids? (A) Lecithin (C) Lysolecithi n (B) Plasmalogen (D) Gangliosides 535. The pH of gastric juice of infants is (A) 2.0 (C) 4.5 (B) 4.0 (D) 5.0 536. The pH of lood is a out 7.4 hen the ratio (A) 10 : 1 (C) 25 : 1 (B) 20 : 1 (D) 30 : 1
et een (NaHCO3) and (H2CO3) is
546. A fatty acid hich is not synthesized in human ody and has to e supplied in the diet is (A) Palmitic acid (C) Linoleic acid (A) (B) (C) (D) (B) Oleic aci d (D) Stearic acid 547. Phospholipids occur in Myelin sheath Sta ilizes chylomicrans Erythrocyte me m rane All of these 537. The a sorption of glucose is decreased C) Thiamine (B) Vitamin D (D) Vitamin B12
y the deficiency of (A) Vitamin A (
106 548. Which of the follo ing is not essential fatty acids? (A) Oleic acid (B) Lin oleic acid (C) Arachidonic acid (D) Linolenic acid 549. The caloric value of lip ids is (A) 6.0 Kcal/g (C) 15.0 Kcal/g (B) 9.0 Kcal/g (D) 12.0 Kcal/g MCQs IN BIOCHEMISTRY 553. Spermatozoa in seminal fluid utilises the follo ing sugar for meta olism: ( A) Galactose (C) Sucrose (B) Glucose (D) Fructose 554. Depot fats of mammalian cells comprise mostly of (A) Cholesterol (C) Cere r osides (B) Phospholipid (D) Triglycerol 550. The maximum num er of dou le (C) 4 (B) 3 (D) 5
onds present in essential fatty acid is (A) 2
555. When choline of lecithin is replaced y ethanolamine, the product is (A) Sp ingomyelin (C) Plasmalogens (B) Cephalin (D) Lysolecithin 551. Prostaglandin synfhesis is increased y activating phospholipases pacrine (C) Glucocorticoids (B) Angiotensin II (D) Indomenthacin
y (A) Me
556. Which of the follo ing is a hydroxyl fatty acid? (A) Oleic Acid (C) Caproic acid (B) Ricinoleic acid (D) Arachidonic acid 552. Sel anof’s test is positive in (A) Glucose (C) Galactose (B) Fructose (D) Man nose 557. Acroleic test is given phingol
y (A) Cholesterol (C) Glycosides (B) Glycerol (D) S
FATS AND FATTY ACID METABOLISM ANSWERS 1. A 7. C 13. B 19. C 25. A 31. A 37. D 43. B 49. C 55. C 61. A 67. A 73 . A 79. B 85. B 91. D 97. B 103. A 109. B 115. D 121. D 127. B 133. C 139. C 145 . B 151. A 157. D 163. D 169. C 175. B 181. C 187. C 193. C 199. A 205. D 211. B 217. C 223. D 229. D 235. C 241. B 107 2. A 8. D 14. A 20. D 26. A 32. A 38. A 44. C 50. C 56. D 62. A 68. B 74. D 80. C 86. B 92. B 98. D 104. B 110. C 116. A 122. A 128. A 134. B 140. B 146. D 152. A 158. D 164. C 170. A 176. C 182. B 188. B 194. C 200. C 206. A 212. A 218. D 224. D 230. B 236. C 242. D 3. C 9. D 15. D 21. C 27. C 33. C 39. B 45. D 51. A 57. A 63. A 69. A 75. B 81. C 87. A 93. A 99. A 105. C 111. D 117. A 123. A 129. B 135. D 141. B 147. C 153. C 159. D 165. D 171. D 177. D 183. C 189. D 195. A 201. A 207. D 213. C 219. A 225. B 231. A 237. D 243. A 4. C 10. B 16. B 22. A 28. B 34. A 40. C 46. A 52. B 58. B 64. D 70. A 76. A 82. A 88. B 94. D 100. A 106. C 112. A 118. D 124. D 130. C 136. A 142. B 148. B 15 4. B 160. C 166. B 172. C 178. B 184. D 190. B 196. D 202. D 208. A 214. D 220. C 226. D 232. A 238. C 244. C 5. D 11. D 17. B 23. D 29. B 35. A 41. D 47. D 53. D 59. D 65. B 71. A 77. B 83. A 89. D 95. B 101. C 107. B 113. A 119. C 125. B 131. B 137. C 143. C 149. A 15 5. D 161. B 167. D 173. A 179. B 185. D 191. C 197. B 203. C 209. C 215. D 221. D 227. D 233. D 239. B 245. C 6. A 12. A 18. D 24. C 30. D 36. C 42. A 48. B 54. B 60. C 66. A 72. B 78. A 84. A 90. C 96. A 102. B 108. A 114. A 120. D 126. A 132. C 138. C 144. D 150. A 15 6. D 162. B 168. B 174. B 180. C 186. D 192. D 198. D 204. B 210. C 216. C 222. C 228. A 234. B 240. D 246. A
108 247. C 253. A 259. A 265. D 271. A 277. D 283. B 289. A 295. C 301. B 307. A 313 . C 319. D 325. B 331. A 337. B 343. A 349. D 355. C 361. A 367. A 373. B 379. A 385. A 391. B 397. D 403. C 409. C 415. A 421. A 427. C 433. C 439. C 445. D 45 1. A 457. B 463. C 469. B 475. D 481. B 487. B 493. A 248. C 254. B 260. A 266. A 272. C 278. C 284. C 290. D 296. B 302. C 308. B 314. A 320. A 326. A 332. C 3 38. A 344. D 350. B 356. C 362. C 368. D 374. B 380. B 386. A 392. D 398. C 404. B 410. C 416. D 422. C 428. B 434. B 440. B 446. B 452. B 458. C 464. B 470. D 476. B 482. B 488. B 494. A 249. A 255. C 261. B 267. D 273. C 279. A 285. A 291 . C 297. B 303. B 309. D 315. D 321. B 327. B 333. A 339. A 345. D 351. A 357. A 363. D 369. C 375. D 381. D 387. A 393. C 399. A 405. D 411. C 417. D 423. B 42 9. A 435. A 441. C 447. D 453. B 459. B 465. A 471. B 477. D 483. D 489. A 495. D 250. A 256. A 262. A 268. C 274. A 280. A 286. C 292. B 298. C 304. C 310. D 3 16. A 322. C 328. C 334. A 340. B 346. D 352. B 358. D 364. B 370. D 376. C 382. B 388. C 394. D 400. C 406. B 412. B 418. C 424. C 430. C 436. C 442. D 448. B 454. D 460. B 466. D 472. D 478. C 484. A 490. B 496. D MCQs IN BIOCHEMISTRY 251. C 257. C 263. C 269. C 275. A 281. D 287. A 293. C 299. B 305. C 311. D 317 . C 323. D 329. B 335. A 341. C 347. A 353. D 359. C 365. A 371. C 377. A 383. A 389. B 395. C 401. B 407. C 413. B 419. D 425. B 431. D 437. A 443. B 449. C 45 5. C 461. D 467. A 473. B 479. A 485. C 491. C 497. C 252. A 258. A 264. A 270. C 276. A 282. C 288. C 294. B 300. A 306. A 312. A 318. B 324. C 330. C 336. D 3 42. C 348. C 354. B 360. B 366. D 372. D 378. C 384. A 390. B 396. B 402. D 408. D 414. D 420. B 426. A 432. B 438. C 444. A 450. A 456. A 462. B 468. A 474. D 480. D 486. D 492. B 498. C
FATS AND FATTY ACID METABOLISM 499. B 505. C 511. D 517. C 523. C 529. B 535. D 541. D 547. D 553. D 500. D 506 . B 512. A 518. A 524. B 530. B 536. B 542. B 548. A 554. D 501. A 507. B 513. A 519. B 525. B 531. D 537. C 543. A 549. B 555. B 502. D 508. D 514. A 520. C 52 6. C 532. C 538. D 544. C 550. C 556. B 503. D 509. D 515. D 521. B 527. D 533. B 539. B 545. D 551. B 557. B 504. A 510. D 516. D 522. A 528. C 534. A 540. D 5 46. C 552. B 109
110 EXPLANATIONS FOR THE ANSWERS 5. D The fatty acids that cannot e synthesized y the ody and therefore should e supplied through the diet are referred to as essential fatty acids (EFA). Li noleic acid and linolenic acid are essential. Some orkers regard arachidonic ac id as an EFA although it can e synthesized from linoleic acid. Phrynoderma (toa d skin) is an essential fatty acid deficiency disorder. It is characterized y t he presence of horny eruptions on the posterior and the lateral parts of the lim s, on the ack and uttocks. The hydrolysis of triacylglycerols y alkali to pr oduce glycerol and soaps is kno n as saponification. Reichert-Meissl num er is d efined as the num er of moles of 0.1 N KOH required to completely neutralize the solu le volatile fatty acids distilled from 5 g fat. Sphingomyelins (sphingopho spholipids) are a group of phospholipids containing sphingosine as the alcohol ( in place of glycerol in other phospholipids). 285. A MCQs IN BIOCHEMISTRY 345. D 61. A 398. C 120. D 454. D 173. A 540. D 231. A Cyclopentanoperhydrophenanthrene (CPPP), it consists of a phenanthrene nucleus t o hich a cyclopentene ring is attached. Cholesterol is an animal sterol ith a molecular formula C27H46O. it has one hydroxyl group at C3 and a dou le ond et een C5 and C6. An 8 car on aliphatic side chain is attached to C17, Cholesterol contains of total 5 methyl groups. The lipids hich possess oth hydropho ic an d hydrophilic groups are kno n as amphipathic lipids (Greek: amphi- oth; pathos - passion). Liposomes have an intermittent aqueous phase in lipid ilayer. They are produced hen amphipathic lipids in aqueous medium are su jected to sonifica tion. Liposomes are used as carriers of drugs to target tissues. Fats (triacygly cerols) are the most predominant storage form of energy, since they are highly c oncentrated form of energy (9 Cal/g) and can e stored in an anhydrous form (no association ith ater).
VITAMINS 111 CHAPTER 5 ITAMINS VITAMINS 1. Vitamins are (A) (B) (C) (D) Accessory food factors Generally synthesized in the ody Produced in endocrine glands Proteins in nature 6. Retinol and retinal are interconverted requiring dehydrogenase or reductase i n the presence of (A) NAD or NADP (C) NADPH (B) NADH + H+ (D) FAD 2. Vitamin A or retinal is a (A) Steroid (B) Polyisoprenoid compound containing a cyclohexenyl ring (C) Benzoquinone derivative (D) 6-Hydroxychromane 3. β-Caroten e, precursor of vitamin A, is oxidatively cleaved y (A) (B) (C) (D) β-Carotene di oxygenase Oxygenase Hydroxylase Transferase 7. Fat solu le vitamins are (A) Solu le in alcohol (B) one or more Propene units (C) Stored in liver (D) All these 8. The international unit of vitamin A is equ ivalent to the activity caused y (A) 0.3 µg of Vitamin A alcohol (B) 0.344 µg of Vi tamin A alcohol (C) 0.6 µg of Vitamin A alcohol (D) 1.0 µg of Vitamin A alcohol 9. L umirhodopsin is sta le only at temperature elo (A) –10°C (C) –40°C (B) –20°C (D) –50°C 4. Retinal is reduced to retinol in intestinal mucosa e reductase utilising (A) NADPH + H (C) NAD +
y a specific retinaldehyd
(B) FAD (D) NADH + H+ 10. Retinol is transported in lood ound to (A) Aporetinol -Glo ulin (C) β-Glo ulin (D) Al umin
inding protein (B) α2
5. Preformed Vit min A is supplied y (A) (B) (C) (D) Milk, f t llow veget les All yellow fruits Le fy green veget les
nd liver All ye
112 11. The norm l serum concentr tion of vit min A in mg/100 ml is (A) 5–10 (C) 100–150 (B) 15–60 (D) 0–5 MCQs IN BIOCHEMISTRY 20. Vit min D sorption is incre sed in (A) (B) (C) (D) Acid pH of intestine Al k line pH of intestine Imp ired f t sorption Contents of diet 12. One m nifest tion of vit min A deficiency is (A) P inful joints (B) Night l indness (C) Loss of h ir (D) Thickening of long ones 13. Deficiency of Vit min A c uses (A) Xeropth lmi (B) Hypoprothrom inemi (C) Meg lo l stic nemi (D) P ernicious nemi 14. An import nt function of vit min A is (A) To ct s coenzym e for few enzymes (B) To pl y n integr l role in protein synthesis (C) To pre vent hemorrh ges (D) To m int in the integrity of epitheli l tissue 15. Retin l is component of (A) Iodopsin (C) C rdiolipin (B) Rhodopsin (D) Glycoproteins 21. The most potent Vit min D met olite is (A) (B) (C) (D) 25-Hydroxycholec lci ferol 1,25-Dihydroxycholec lciferol 24, 25-Dihydroxycholec lciferol 7-Dehydrocho lesterol 22. The norm l serum concentr tion of 25-hydroxycholec lciferol in ng/ml is (A) 0–8 (C) 100–150 (B) 60–100 (D) 8–55 23. The norm l serum concentr tion of 1,25dihydroxycholec lciferol in pg/ml is ( A) 26–65 (C) 5–20 (B) 1–5 (D) 80–100 24. The norm l serum concentr tion of 24,25dihydroxycholec lciferol in ng/ml is (A) 8–20 (C) 1–5 (B) 25–50 (D) 60–100 25. A poor source of Vit min D is (A) Egg (C) Milk (B) Butter (D) Liver 16. Retinoic cid p rticip tes in the synthesis of (A) Iodopsin (C) Glycoprotein (B) Rhodopsin (D) C rdiolipin 26. Richest source of Vit min D is (A) Fish liver oils (C) Egg yolk (B) M rg rin e (D) Butter 17. On exposure to light rhodopsin forms (A) All tr ns-retin l (C) Retinol (B) C is-retin l (D) Retinoic cid 27. Deficiency of vit min D c uses (A) (B) (C) (D) Ricket culosis of one Hypthyroidism Skin c ncer
nd osteom l ci
18. C rr-Price re ction is used to detect (A) Vit min A (C) Ascor ic t min D (D) Vit min E
Tu er
cid (B) Vi
19. The structure shown elow is of (A) Cholec lciferol (B) 25-Hydroxycholec lci ferol (C) Ergoc lciferol (D) 7-Dehydrocholesterol 28. One intern tion l unit (I.U) of vit min D is defined s the iologic l ctiv ity of (A) (B) (C) (D) 0.025 µg of cholec lciferol 0.025 µg of 7-dehydrocholec lcife rol 0.025 µg of ergosterol 0.025 µg of ergoc lciferol
VITAMINS 29. The β-ring of 7-dehydrocholesterol is cle ved to form cholec lciferol y (A) ( B) (C) (D) Infr red light Dim light Ultr violet irrid tion with sunlight Light o f the tu e lights 113 38. All the following conditions produce re l or function l deficiency of vit min K except (A) Prolonged or l, ro d spectrum nti iotic ther py (B) Tot l l c k of red me t in the diet (C) The tot l l ck of green le fy veget les in the di et (D) Being new orn inf nt 39. Vit min K is found in (A) Green le fy pl nts (B) Me t (C) Fish (D) Milk 40. Function of Vit min A: (A) (B) (C) (D) He ling ep itheli l tissues Protein synthesis regul tion Cell growth All of these (B) Whe t gr m (D) Putrid fish me l 30. C lcitriol synthesis involves (A) (B) (C) (D) Both liver e Adipose tissue Muscle
nd kidney Intestin
31. Insignific nt mount of Vit min E is present in (A) Whe t germ oil (B) Sunfl ower seed oil (C) S fflower seed oil (D) Fish liver oil 32. The ctivity of toco pherols is destroyed y (A) (B) (C) (D) Commerci l cooking Reduction Conjug tion All of these 41. Vit min K2 w s srcin 42. Vit min synthesized
lly isol ted from (A) Soy y
e n (C) Alf
Alf
cteri l in the intestine is (A) A (C) D (B) C (D) K
33. The requirement of vit min E is incre sed with gre ter int ke of (A) (B) (C) (D) C r ohydr tes Proteins Polyuns tur ted f t S tur ted f t 43. Vit min K is involved in posttr nsl tion l modific tion of the lood clottin g f ctors y cting s cof ctor for the enzyme: (A) C r oxyl se (C) Hydroxyl se (B) Dec r oxyl se (D) Oxid se 34. Vit min E reduces the requirement of (A) Iron (C) Selenium (B) Zinc (D) M gn esium 44. Vit min K is cof ctor for (A) G mm c r oxyl tion of glut mic cid residue (B) β-Oxid tion of f tty cid (C) Form tion of γ amino butyrate (D) Synthesis of tr yptophan 45. Hypervitaminosis K in neonates may cause (A) Porphyria (C) Pella ra (A) Riboflavin (C) Menadione (B) Jaundice (D) Prolon ed bleedin (B) Retinol (D ) Tocopherol 35. The most important natural antioxidant is (A) Vitamin D (C) Vitamin B12 (A) Vitamin A (C) Vitamin K (B) Vitamin E (D) Vitamin K 36. Tocopherols prevent the oxidation of (B) Vitamin D (D) Vitamin C 37. Creatinuria is caused due to the deficiency of vitamin (A) A (C) E (B) K (D) D 46. Dicoumarol is anta onist to
114 47. In the individuals who are iven liberal quantities of vitamin C, the serum ascorbic acid level is (A) (B) (C) (D) 1–1.4 µ /100 ml 2–4 µ /100 ml 1–10 µ /100 ml 10–20 ml MCQs IN BIOCHEMISTRY 56. Both Wernicke’s disease and beriberi can be reversed by administratin (A) Ret inol (C) Pyridoxine (A) Ricket (C) Beriberi (B) Thiamin (D) Vitamin B12 (B) Nyct alopia (D) Pella ra 57. The Vitamin B1 deficiency causes 48. The vitamin which would most likely become deficient in an individual who de velop a completely carnivorous life style is (A) Thiamin (C) Vitamin C (B) Niaci n (D) Cobalamin 58. Concentration of pyruvic acid and lactic acid in blood is increased due to d eficiency of the vitamin (A) Thiamin (C) Niacin (A) (B) (C) (D) (B) Riboflavin ( D) Pantothenic acid 49. In human body hi hest concentration of ascorbic acid is found in (B) Adrenal cortex (A) Liver (C) Adrenal medulla (D) Spleen 50. The vitamin required for th e formation of hydroxyproline (in colla en) is (A) Vitamin C (C) Vitamin D (B) V itamin A (D) Vitamin E 59. Vitamin B1 coenzyme (TPP) is involved in Oxidative decarboxylation Hydroxyla tion Transamination Carboxylation 60. Increased lucose consumption increases the dietary requirement for (A) Pyri doxine (C) Biotin (B) Niacin (D) Thiamin 51. Vitamin required for the conversion of phydroxyphenylpyruvate to homo entisa te is (A) Folacin (C) Ascorbic acid (B) Cobalamin (D) Niacin 61. Thiamin is oxidized to thiochrome in alkaline solution by (A) (B) (C) (D) Po tassium perman anate Potassium ferricyanide Potassium chlorate Potassium dichrom ate 52. Vitamin required in conversion of folic acid to folinic acid is (A) Biotin ( C) Ascorbic acid (B) Cobalamin (D) Niacin 62. Riboflavin is a coenzyme in the reaction catalysed by the enzyme (A) (B) (C) (D) Acyl CoA synthetase Acyl CoA dehydro enase β-Hydroxy acyl CoA Enoyl CoA dehyd rogenase 53. Ascor ic acid can reduce (A) (B) (C) (D) 2, 6-Di romo enzene 2, 6-Diiodoxypy ridine 2, 6-Dichlorophenol indophenol 2, 4-Dinitro enzene 63. The daily requirement of ri oflavin for adult in mg is (A) 0–1.0 (C) 2.0–3.5 (B) 1.2–1.7 (D) 4.0–8.0 54. Sterilised milk lacks in (A) Vitamin A (C) Vitamin C (B) Vitamin D (D) Thiam in 55. Scurvy is caused due to the deficiency of (A) Vitamin A (C) Vitamin K (B) Vi tamin D (D) Vitamin C 64. In ne orn infants phototherapy may cause hyper iliru inemia ith deficienc y of (A) Thiamin (C) Ascor ic acid (B) Ri oflavin (D) Pantothenic acid
VITAMINS 65. Ri oflavin deficiency causes (A) (B) (C) (D) Cheilosis Loss of deterioration Dermatitis
eight Mental
115 75. Pellagra occurs in population dependent on (A) Wheat (C) Maize (B) Rice (D) Milk 66. Magenta tongue is found in the deficiency of the vitamin (A) Ri oflavin (C) Nicotinic acid (B) Thiamin (D) Pyridoxine 76. The enzymes ith hich nicotinamide act as coenzyme are (A) Dehydrogenases ( B) Transaminases (C) Decar oxylases (D) Car oxylases 77. Dietary requirement of Vitamin D: (A) 400 I.U. (C) 6000 I.U. (B) 1000 I.U. (D) 700 I.U. 67. Corneal vascularisation is found in deficiency of the vitamin: (A) B1 (C) B3 (A) Ri oflavin (C) Niacin (B) B2 (D) B6 (B) Pantothenic acid (D) Pyridoxine 78. The Vitamin hich does not contain a ring in the structure is (A) Pantotheni c acid (B) Vitamin D (C) Ri oflavin (D) Thiamin 79. Pantothenic acid is a consti tuent of the coenzyme involved in (A) Decar oxylation (C) Acetylation (B) Dehydr ogenation (D) Oxidation 68. The pellagra preventive factor is 69. Pellagra is caused due to the deficiency of (A) Ascor ic acid (C) Pyridoxine (B) Pantothenic acid (D) Niacin 80. The precursor of CoA is (A) Ri oflavin (C) Thiamin (B) Pyridoxamine (D) Pant othenate 70. Niacin or nicotinic acid is a monocar oxylic acid derivative of (A) Pyridine (C) Flavin (B) Pyrimidine (D) Adenine 81. ‘Burning foot syndrome’ has een ascri ed to the deficiency of (A) Pantothenic a cid (B) Thiamin (C) Co alamin (D) Pyridoxine 82. Pyridoxal phosphate is central to (A) Deamination (C) Car oxylation (B) Amidation (D) Transamination 71. Niacin is synthesized in the sine (D) Aspartate
ody from (A) Tryptophan (C) Glutamate (B) Tyro
72. The proteins present in maize are deficient in (A) Lysine (C) Tryptophan (B) Threonine (D) Tyrosine 73. Niacin is present in maize in the form of (A) Niatin (C) Niacytin (B) Nicoti n (D) Nicyn 83. The vitamin required as coenzyme for the action of transaminases is (A) (B) (C) (D) Niacin Pantothenic acid Pyridoxal phosphate Ri oflavin 74. In the ody 1 mg of niacin can e produced from (A) (B) (C) (D) 60 mg of pyr idoxine 60 mg of tryptophan 30 mg of tryptophan 30 mg of pantothenic acid 84. Vitamin B6 deficiency may occur during therapy drugs (B) Terramycin (D) Aspirin
ith (A) Isoniazid (C) Sulpha
116 85. Deficiency of vitamin B6 may occur in (A) O ese person (C) Alcoholics (B) Th in person (D) Dia etics MCQs IN BIOCHEMISTRY 93. The cofactor or its derivative required for the conversion of acetyl CoA to malonylCoA is (A) FAD (C) NAD+ (B) ACP (D) Biotin 86. ‘Xanthurenic acid index’ is a relia le criterion for the deficiency of the vitam in (A) Pyridoxal (B) Thiamin (C) Pantothenic acid (D) Co alamin 87. Epileptiform convulsion in human infants have een attri uted to the deficiency of the vitam in (A) B1 (C) B6 (A) Car oxylase (C) Decar oxylase (B) B2 (D) B 12 (B) Hydroxyla se (D) Deaminase 94. A cofactor required in oxidative decar oxylation of pyruvate is (A) (B) (C) (D) Lipoate Pantothenic acid Biotin Para amino enzoic acid 95. The central structure of B12 referred to as corrin ring system consists of ( A) Co alt (C) Magnesium (B) Manganese (D) Iron 88. Biotin is a coenzyme of the enzyme 96. The central heavy metal co alt of vitamin B12 is coordinately ound to (A) C yanide group (C) Car oxyl group (B) Amino group (D) Sulphide group 89. The coenzyme required for conversion of pyruvate to oxaloacetate is (A) FAD (C) TPP (B) NAD (D) Biotin 97. Vitamin B12 has a complex ring structure (corrin ring) consisting of four (A ) Purine rings (C) Pyrrole rings (A) (B) (C) (D) (A) (B) (C) (D) (B) Pyrimidine rings (D) Pteridine rings 90. In iotin-containing enzymes, the iotin is ound to the enzyme y (A) An am ide linkage to car oxyl group of glutamine (B) A covalent ond ith CO2 (C) An a mide linkage to an amino group of lysine (D) An amide linkage to α-c r oxyl group of protein 91. A molecule of CO2 is c ptured y iotin when it cts s coenzyme for c r oxyl tion re ction. The c r oxyl group is cov lently tt ched to (A) A n itrogen (N1) of the iotin molecule (B) Sulphur of thiophene ring (C) α-Amino grou p of lysine (D) α-Amino group of protein 92. Consumption of r w eggs c n c use def iciency of (A) Biotin (C) Ri ofl vin (B) P ntothenic cid (D) Thi min 98. Emperic l formul of co l min is C63H 88N 12O14P.CO C61H 82N 12O12P.CO C61H 88N 12O14P.CO C63H 88N 14O14P.CO Beri-Beri Scurvy Perniciuos nemi Ricket 99. A deficiency of vit min B12 c uses 100. Vit min B12 deficiency c n e di gnosed e (C) M l te (B) Methylm lon te (D) L ct te
y urin ry excretion of (A) Pyruv t
101. Su cute com ined degener tion of cord is c used due to deficiency of (A) N i cin (C) Biotin (B) Co l min (D) Thi min
VITAMINS 102. Vit min required for met olism of diols e.g. conversion of ethylene glycol to cet ldehyde is (A) Thi min (C) Pyridoxine (B) Co l min (D) Folic cid 110. Thi min deficiency includes (A) Ment l depression (B) F tigue (C) Beri eri (D) All of these 117 111. Thi min diphosph te is required for oxid tive dec r oxyl tion of (A) α-Keto cids (C) F tty cids (A) (B) (C) (D) (A) (B) (C) (D) (A) (B) (C) (D) Unpolished rice P r oiled rice Whole whe t flour All of these 0.1 mg/1,000 C lories 0.5 mg/ 1,000 C lories 0.8 mg/1,000 C lories 1.0 mg/1,000 C lories Non-veget ri ns Alcoh olics Pregn nt women Both B nd C (B) α-Amino cids (D) All of these 103. Both folic cid nd methyl co l min (vit min B12) re required in (A) De m in tion of serine (B) De min tion of threonine (C) Conversion of pyridox l phosp h te to pyridox mine phosph te (D) Methyl tion of homocystein to methionine 104. Folic cid or fol te consists of the (A) B se pteridine, p- mino enzoic cid sp r te (B) B se purine, p- mino enzoic cid glut m te (C) B se pteridine, p- m ino enzoic cid glut m te (D) B se purine, p-hydroxy enzoic cid glut m te nd nd nd nd 112. Loss of thi min c n
e decre sed
y using
113 . D ily requirement of thi min is 114. Thi min requirement is gre ter in 105. Fol te s coenzyme is involved in the tr nsfer nd utiliz tion of (A) (B) (C) (D) Amino group Hydroxyl group Single c r on moiety Amido group 115. People consuming polished rice s their st ple food re prone to (A) Beri e ri (C) Both (A) nd (B) (A) Acidic medium (C) Neutr l medium (B) Pell gr (D) No ne of these (B) Alk line medium (D) Both (A) nd (C) 106. Folic cid deficiency c n e di gnosed y incre sed urin ry excretion of (A ) Methylm lon te (C) Cyst thionine (B) Figlu (D) Cre tinine 116. Ri ofl vin is he t st
le in
107. Sulph drugs interfere with cteri l synthesis of (A) Lipo te (C) Tetr hyd rofol te (A) (B) (C) (D) (B) Vit min E (D) Ascor ic cid 117. FAD is coenzyme for (A) (B) (C) (D) Succin te dehydrogen se Glycerol-3-ph osph te dehydrogen se Sphingosine reduct se All of these 108. Fol te deficiency c uses Microcytic nemi Hemolytic nemi Iron deficiency nemi Meg lo l stic nemi (B) Alk line medium (D) None of these 118. Ri ofl vin deficiency c n c use (A) Peripher l neuritis (B) Di rrhoe (C) A ngul r stom titis (D) None of these 119. Pell gr preventing f ctor is (A) Thi m in (C) Ni cin (B) Ri ofl vin (D) Pyridoxine 109. Thi min is he t st
le in (A) Acidic medium (C) Both (A)
nd (B)
118 120. Ni cin cont ins (A) Sulphydryl group (B) C r oxyl group (C) Amide group ( D) All of these 121. NADP is required s coenzyme in (A) Glycolysis (C) HMP sh unt (A) (B) (C) (D) (A) (B) (C) (D) (B) Citric cid cycle (D) Gluconeogenesis MCQs IN BIOCHEMISTRY 130. Sulphydryl group of coenzyme is contri uted y (A) β-Al nine (B) β-Aminoiso u tyric cid (C) Methionine (D) Thioeth nol mine 131. Coenzyme A cont ins nitrog enous se which is (A) Adenine (C) Choline (B) Gu nine (D) Eth nol mine 122. NAD is required s coenzyme for M l te dehydrogen se Succin te dehydrogen se Glucose-6-phosph te dehydrogen se HMG CoA reduct e Citric cid cycle HMP shu nt β-Oxid tion of f tty cids Both (A) nd (C) 123. NAD is required
s
conenzyme in
132. The following is required for the form tion of coenyzme A: (A) ATP (C) CTP (B) GTP (D) None of these 133. Coenzyme A is required for c t ine (D) All of these
olism of (A) Leucine (C) V line (B) Isoleuc
124. Ni cin c n e synthesised in hum n eings from (A) Histidine (C) Tyrosine ( A) 5 mg (C) 20 mg (B) Phenyl l nine (D) Tryptoph n (B) 10 mg (D) 30 mg 134. Deficiency of p ntothenic cid in hum n eings c n ffect (A) Nervous syste m (C) Both (A) nd (B) (B) Digestive system (D) None of these 125. D ily requirement of ni cin is 135. Pyridox l phosph te is coenzyme for (A) Glut m te ox lo cet te tr ns min se (B) Glut m te pyruv te tr ns min se (C) Tyrosine tr ns min se (D) All of thes e 136. Pyridox l phosph te is required s coenzyme in (A) Tr ns min tion (C) D esulphydr tion (B) Tr nsulphur tion (D) All of these 126. Ni cin deficiency is common in people whose st ple food is (A) (B) (C) (D) (A) (B) (C) (D) Whe t Polished rice M ize nd /or sorghum None of these Exposed p rts of ody Covered p rts of ody Trunk only All p rts of the ody 127. In pell gr , derm titis usu lly
ffects
137. Pyridox l phosph te is coenzyme for (A) Glycogen synthet se (B) Phosphory l se (C) Both (A) nd (B) (D) None of these 138. Pyridoxine deficiency c n e di gnosed y me suring urin ry excretion of (A) Pyruvic cid (B) Ox lo cetic cid (C) X nthurenic cid (D) None of these 128. Ni cin deficiency c n occur in (A) H rtnup dise se (B) Phenylketonuri (C) Alk ptonuri (D) None of these 129. P ntothenic cid cont ins n mino cid whic h is (A) Asp rtic cid (C) β-Al nine (B) Glut mic cid (D) β-Aminoiso utyric cid
VITAMINS 139. Pyridoxine deficiency c n e di gnosed y me suring the urin ry excretion o f x nthurenic cid following test dose of (A) Glycine (C) Tryptoph n (B) Histi dine (D) Pyridoxine 147. Folic cid cont ins (A) Pteridine (B) p-Amino enzoic cid (C) Glut mic cid (D) All of these 119 140. Pyridoxine requirement depends upon the int ke of (A) C r ohydr tes (C) F t s (B) Proteins (D) None of these 148. Conversion of fol te into tetr hydrofol te requires (A) NADH (C) FMNH2 (A) Glossitis (C) Vomitting (B) NADPH (D) FADH2 (B) stom tis (D) Both (A) nd (B) 141. Anti-egg white injury f ctor is (A) Pyridoxine (C) Thi min (B) Biton (D) Li ponic cid 149. Ri ofl vin deficiency symptoms
re
142. When eggs re cooked (A) Biotin is destroyed ut vidin rem ins un ffected (B) Avidin is in ctiv ted ut iotin rem ins un ffected (C) Both vidin nd iot in re in ctiv ted (D) Both vidin nd iotin rem in un ffected 143. Biotin is r equired s coenzyme y (A) An ero ic dehydrogen ses (B) Dec r oxyl ses (C) Aer o ic dehydrogen ses (D) C r oxyl ses 144. Biotin is coenzyme for (A) Pyruv te c r oxyl se (B) Acetyl CoA c r oxyl se (C) Propionyl CoA c r oxyl se (D) All of these 145. Lipoic cid is conenzyme for (A) Pyruv te dehydrogen se (B) α-Ketoglu t r te dehydrogen e (C) Both (A) nd (B) (D) None of these 146. Chemic lly, lipo ic cid is (A) S tur ted f tty cid (B) Uns tur ted f tty cid (C) Amino cid (D ) Sulphur cont ining f tty cid 150. Vit min B12 forms coenzymes known s (A) Co mide (B) Tr nsco l min I (C) Tr nsco l min II (D) Both (B) nd (C) 151. Methylco l min is required for form tion of (A) (B) (C) (D) Serin from glycine Glycine from serine Methionine from homocysteine All of these 152. A sorption of Vit min B12 requires the presence of (A) Pepsin (C) Intrinsic f ctor (B) Hydrochloric cid (D) Boh (B) nd (C) 153. Intrinsic f ctor is chemic lly olys cc ride Peptide
(A) (B) (C) (D) Protein Glycoprotein Mucop
154. Chemic lly, Extrinsic F ctor of C stle is coprotein Mucopolys cch ride Cy noco l minm
(A) (B) (C) (D) Mucoprotein Gly
155. Vit min B12 is (A) (B) (C) (D) Not stored in the Stored in liver Stored in RE cells
ody Stored in
one m rrow
120 156. Vit min B12 is tr nsported in lood y (A) Al umin (B) Tr nscortin (C) Tr n sco l min I (D) Tr nsco l min II 157. Vit min B12 is synthesized y (A) B cter i only (C) Anim ls only (B) Pl nts only (D) Both (A) nd (C) MCQs IN BIOCHEMISTRY 165. Deficiency of vit min C c uses (A) (B) (C) (D) Beri eri Pell gr n emi Scurvy
Pernicious
158. Deficiency of vit min B12 c n occur ec use of (A) (B) (C) (D) Decre sed in t ke of vit min B12 Atrophy of g stric mucos Intestin l m l sorption All of th ese 166. An e rly di gnosis of vit min C deficiency c n e m de y (A) (B) (C) (D) M e suring pl sm scor ic cid Me suring urin ry scor ic cid Ascor ic cid s tu r tion test All of these 159. Deficiency of vit min B12 c n e di gonised y (A) (B) (C) (D) C rr-Price r e ction Ames ss y W tson-Schw rtz test Schilling test 167. D ily requirement of vit min C in 25 mg (D) 100 mg
dults is
out (A) 100 mg (C) 70 mg (B)
168. The vit min h ving the highest d ily requirement mong the following is (A) Thi min (C) Pyridoxine (B) Ri ovfl vin (D) Ascor ic cid 160. G styrectomy le ds to meg lo l stic s (B) Weeks (D) Ye rs
n emi
within
few (A) D ys (C) Month
169. An emi c n occur due to the deficiency of ll the following except (A) Thi min (C) Folic cid (B) Pyridoxine (D) Cy noco l min 161. Ascor ic cid is required to synthesise ll of the following except (A) Col l gen (C) Bile pigments (B) Bile cids (D) Epinephrine 170. A vit min which c n e synthesized cid (B) Ni cin (D) Cy noco l min
y hum n
eings is (A) Thi min (C) Folic
162. Vit min C enh nces the intestin l Iodine (D) None of these
sorption of (A) Pot ssium (C) Iron (B)
171. L or tory di gnosis of vit min B 12 deficiency c n e m de y me suring th e urin ry excretion of (A) (B) (C) (D) X nthurenic cid Formiminoglut mic cid M ethylm lonic cid Homogentisic cid (B) β-Ionone ring (D) None of these (B) β-C rote ne (D) All of these 163. Vit min C ctivity is present in (A) (B) (C) (D) D-Ascor ic scor ic cid L-Ascor ic cid Both A nd B
cid D-Dehydro
172. The molecule of vit min A1 cont ins (A) Benzene ring (C) β-C rotene ring (A) αC rotene (C) γ Carotene 164. Vitamin C is required for the synthesis of (A) (B) (C) (D) Bile acids from cholesterol Bile salts from bile acids Vitamin D from cholesterol All of these 173. Precursor of Vitamin A is
VITAMINS 174. Two molecules of vitamin A can be formed from 1 molecule of (A) α-C rotene (C ) γ Carotene (B) β-Carotene (D) All of these 183. Retinol isomerase is present in (A ) Retina (C) Both (A) and (B) (A) β-Carotene (C) Retinoic acid (B) Liver (D) None of these (B) Retinol (D) All of these 121 184. Anti-oxidant activity is present in 175. Conversion of β-carotene into retinal requires the presence of (A) (B) (C) (D ) β-Carotene dioxygenase Bile salts Molecular oxygen All of these 185. One international Unit of vitamin A is the activity present in (A) (B) (C) (D) 0.3 µg of β-Carotene 0.3 µg of retinol 0.6 µg of retinoic acid All of these 176. Conversion of retinal into ritonal requires the presence of (A) NADH (C) FA DH2 (B) NADPH (D) Lipoic acid 186. Daily requirement of vitamin A in an adult man can e expressed as (A) 400 IU (C) 5,000 IU 187. Vitamin B6 includes (A) Pyridoxal (C) Pyridoxine (A) (B) (C ) (D) (A) (B) (C) (D) (B) Pyridoxamine (D) All of these (B) 1,000 IU (D) 10,000 IU 177. Retinal is converted into retinoic acid in the presence of (A) Retinal oxid ase (B) Retinal car oxylase (C) Retinene reductase(D) Spontaneously 178. Vitamin A a sor ed in intestine is released into (A) Portal circulation (B) Lacteals (C ) Both (A) and (B) (D) None of these 179. Vitamin A is stored in the ody in (A) (B) (C) (D) Liver Adipose tissue Reticuloendothelial cells All of these 188. An early effect of vitamin a deficiency is Xerophthalmia Keratomalacia Prol onged dark adaptation time Follicular hyperkeratosis Drying of eyes Destruction of cornea Blindness Ina ility to see in dimlight 189. Nyctalopia is 180. Rhodopsin contains opsin and (A) 11-cis-retinal (C) All-cis-retinal (B) 11trans-retinal (D) All trans-retinal 190. Rod cells possess a trans-mem rane protein hich is (A) Adenylate cyclase ( B) Transducin (C) Rhodopsin (D) B as ell as C 191. Provitamins A include (A) Re tinal (C) Carotenes 192. Retinoic acid can (A) (B) (C) (D) Act as a photo recept or Support gro th and differentiation Act as an anti-oxidant None of these (B) R etionic acid (D) All of these 181. When light falls on rod cells (A) All-cis-retinal is converted into all-tra ns-retinal (B) 11-cis-retinal is converted into 11-trans-retinal (C) 11-trans-re tinal is converted into all-transretinal (D) 11-cis-retinal is converted into al l-trans-retinal 182. Conversion of all-trans-retinal into alltrans-retinol requi res (A) NAD (C) NADP (B) NADH (D) NADPH
122 193. Prosthetic group in cone cell phototreceptors is (A) Iodine (C) 11-cis-reti nal (B) Opsin (D) all-trans-retinal MCQs IN BIOCHEMISTRY 202. Calcitriol inhi its the conversion of (A) Cholesterol into 7-dehydrocholest erol (B) Cholecalciferol into 1-hydroxycholecalciferol (C) Cholecalciferol into 25-hydroxycholecalciferol (D) 25-Hydroxycholecalciferol into 1,25- dihydroxychol ecalciferol 203. Bo legs and knock-knees can occur in (A) Rickets (C) Both A and B (B) Osteomalacia (D) Hypervitaminosis D 194. Retinoic acid is involved in the synthesis of (A) Rhodopsin (C) Porphyrinop sin 195 Transducin is a (A) Signal transducer (B) Stimulatory G-protein (C) Trim er (D) All of these 196. Provitamin D3 is (A) (B) (C) (D) Cholecalciferol Ergost erol 7-Dehydrocholesterol Ergocaliferol (B) Iodopsin (D) Glycoproteins 204. Calcification of soft tissues can occur in (A) (B) (C) (D) Osteomalacia Ric kets Hypervitaminosis D None of these 197. Ergosterol is found in (A) Animals (C) Bacteria (B) Plants (D) All of these 205. Levels of serum calcium and inorganic phosphorus are increased in (A) Hyper vitaminosis D (B) Hypoparathyroidism (C) Hypovitaminosis D (D) None of these 206 . Requirement of vitamin E increases ith the increasing intake of (A) Calories (C) PUFA (B) Proteins (D) Cholesterol 198. A provitamin D synthesized in human eings is (A) (B) (C) (D) Ergosterol 7Dehydrocholesterol Cholecalciferol 25-Hydroxycholecalciferol 199. 25-Hydroxylation of vitamin D occurs in (A) Skin (C) Kidneys (B) Liver (D) Intestinal mucosa 207. In human eings, vitamin E prevents (A) Sterility (B) Hepatic necrosis (C) Muscular dystrophy (D) None of these 208. Vitamin E protects (A) Polyunsaturated fatty acids against aperoxidation (B) Vitamin A and carotenes against oxidation (C) Lung tissue against atmospheric pollutants (D) All of these 209. Intestinal acteria can synthesise (A) Phyllogquinone (C) Both (A) and (B) (B) Farnoquinon e (D) Menadione 200. Tu ular rea sorption of calcium is increased y (A) (B) (C) (D) Cholecalcif erol 25-Hydroxycholecalciferol Calcitriol All of these 201. Parathormone is required for the conversion of (A) Cholecalciferol into 1-h ydroxycholecalciferol (B) Cholecalciferol into 25-hydroxycholecalciferol (C) 25Hydroxycholecalciferol into calcitriol (D) Cholesterol into 7-dehydrocholesterol
VITAMINS 210. A ater solu le form of vitamin K is (A) Phylloquinone (C) Menadione (B) Fa rnoquinone (D) None of these 123 220. The performed Vitamin A is supplied ver oil (B) Eggs (D) All of these
y foods such as (A) Butter (C) Fish li
211. Prothrom in time is prolonged in (A) Vitamin K deficiency (B) Liver damage (C) Both (A) and (B) (D) None of these 212. A synthetic form of vitamin K is (A) Menadione (C) Phylloquinone (B) Farnoquinone (D) None of these 221. The non-protein part of rhodopsin is (A) Retinal (C) Carotene (B) Retinol ( D) Repsin 222. Lumirhodopsin is sta le only at a temperature
elo
(A) –35°C (C) –45°C (B) –40°C (D)
213. Retinal is reduced to retinol y retinene reductase in presence of the coen zyme (A) NAD+ (C) NADH + H+ (B) NADP+ (D) NADPH + H+ 223 The normal concentration of vitamin A in ) 24–60 (D) 35–70 214. Retinal exists as an ester g (B) Kidney (D) All of these
lood in I.V/dl: (A) 20–55 (C) 30–65 (B
ith higher fatty acids in the (A) Liver (C) Lun
224. Continued intake of excessive amounts of vitamin A especially in children p roduces (A) Irrita ility (C) Headache (A) (B) (C) (D) (B) Anorexia (D) All of th ese 225. Vitamin D2 is also said to e Activated ergosterol Fergocalciferol Viostero l All of these (B) Butter (D) Liver 215. Retinol is transported to the ) β-glo ulin (B) α2-glo ulin (D) γ
lood as retinol attached to (A) α1-glo ulin (C lobulin
216. Carotenes are transported with the (A) Minerals (C) Lipids (B) Proteins (D) Lipoproteins 226. The poor sources of vitamin D: (A) E
s (C) Milk
217. The dru s that form complexes with pyridoxal are (A) Isoniazid (C) Rifampic in (B) Penicillamine (D) Both (A) and (B) 227. The activity of tocopherols is destroyed by (A) Oxidation (C) Conju ation ( B) Reduction (D) All of these 218. In the blood the vitamin esters are attached to (A) α1-lipoproteins (C) β-lipop roteins (B) α2-lipoproteins (D) γ lipoproteins 228 Some tocopherols are (A) (B) (C) (D) Terpenoid in structure Dional in struct ure Isoprenoid in structure Farnesyl in structure 219. The percenta e of Vitamin A in the form of esters is stored in the liver: ( A) 80 (C) 90 (B) 85 (D) 95 229. The methyl (B) 3 (D) 5
roups in the aromatic nucleus of a tocopherols are (A) 2 (C) 4
124 230. Vitamin E stored in (A) Mitochondria (C) Both (A) and (B) (B) Microsomes (D ) None of these MCQs IN BIOCHEMISTRY 240. The number of nutritionally essential amino acids for man is (A) 6 (C) 10 2 41. Avidin is present in (A) (B) (C) (D) (A) (B) (C) (D) Cow’s milk Raw e Green leafy ve etables Carrots Proteins Proteins and calories Proteins and vitamins Pr oteins and minerals (B) 8 (D) 12 231. Vitamin E protects the polyunsaturated fatty acids from oxidation by molecu lar oxy en in the formation of (A) Superoxide (C) Trioxide (A) Vitamin A (C) Vit amin K (B) Peroxide (D) All of these (B) Vitamin D (D) Vitamin C 232. The tocopherols prevent the oxidation of 242. Marasmus is due to malnutrition of 233. Vitamin E protects enzymes from destruction in (A) Muscles (C) Gonads (B) N erves (D) All of these 234. Vitamin K re ulates the synthesis of blood clottin (A) (B) (C) (D) (B) IX (D) All of these 243. Ener y value in kilocalorie per 4 (D) 18
factors: (A) VII (C) X
ram of fat in the body is (A) 1 (C) 9 (B)
235. Ascorbic acid can reduce 2, 4 dinitro benzene 2, 6 Dichlorophenol Indopheno l 2, 4 dibromobenzene 2, 6 dibromo benzene (B) Vitamin B1 (D) Vitamin D 244. Which amon the followin is an essential amino acid for man? (A) Alanine ( C) Valine (B) Serine (D) Glutamic acid 245. Under what condition to basal metabolic rate environment Hot environment Intake of base formin
oes up? (A) (B) (C) (D) Cold foods Hypothyroidism
236. Sterilized milk is devoid of (A) Vitamin A (C) Vitamin C (A) (B) (C) (D) (A ) (B) (C) (D) 237. The symptoms of scurvy are Poor healin of wounds Loosenin of teeth Anaemi a All of these Vitamin A deficiency Vitamin D deficiency Deficiency of minerals in diet Protein and caloric deficiency in diet 246. What is the major form of caloric stora e in human body? (A) (B) (C) (D) AT P Glyco en Creatine phosphate triacyl lycerol (B) Lacto lobulin (D) Caein 238. Kwashiorkor results from 247. The phosphoprotein of milk is (A) Lactalbumin (C) Vitellin 239. Which amon the followin fatty acids is an essential fatty acid for man? ( A) Palmitic acid (C) Linoleic acid (B) Oleic acid (D) None of these 248. Dictary deficiency of this vitamin leads to ni ht blindness: (A) Retinol (C ) Ascorbic acid (B) Niacin (D) Cholecalciferol
VITAMINS 249. A non essential amino acid is not (A) (B) (C) (D) Absorbed in the intestine s Required in the diet Incorporated into the protein Metabolized by the body 125 257. Milk contains very poor amounts of (A) Calcium (C) Iron (A) (B) (C) (D) (B) Phosphate (D) Riboflavin 258. E
contains very little Fat Proteins Carbohydrates Calcium and phosphorus
250. The deficiency of Vitamin B12 leads to (A) (B) (C) (D) Pernicious anaemia M e ablastic anaemia Both (A) and (B) None of these 259. BMR (Basal Metabolic rate) is elevated in (A) Hyper thyroidism (B) Under nu trition (C) Starvation (D) Hypothyroidism 260. Soyabean proteins are rich in (A) Lysine (C) Glcyine (A) Lysine (C) Glycine (B) Alanine (D) Aspartic acid (B) Ala nine (D) Aspartic acid 251. Which amon the followin is a nutritionally essential amino acid for man? (A) Alanine (C) Tyrosine (B) Glycine (D) Isoleucine 252. The maximum specific dynamic action of food stuff is exerted by (A) carbohy drates (C) proteins (B) fats (D) vitamins 261. Corn and
liadin are low in
253. The essential amino acids (A) must be supplied in the diet because the or a nism has lost the capacity to aminate the correspondin ketoacids (B) must be su pplied in the diet because the human has an impaired ability to synthesize the c arbon chain of the correspondin ketoacids (C) are identical in all species stud ied (D) are defined as these amino acids which cannot be synthesized by the or a nism at a rate adequate to meet metabolic requirements 254. Fibre in the diet is beneficial in (A) (B) (C) (D) Hyper lycemia Hyper cholseteremia Colon cancer A ll of these 262. What is the disease caused by thiamine deficiency? (A) Nycalopia (C) Ricket s (B) Scurvy (D) Beriberi 263. Retinol and retinol –bindin protein (RBP) bound with this protein: (A) Album in (C) α2-glo ulin (B) Pre l umin (D) β-glo ulin 264. Meg lo l stic nemi is c used y the deficiency of (A) Folic cid (C) Iron (A) Vit min A (C) Vit min E 266. C lcitriol is (A) (B) (C) (D) 1-OH-cholec lcif erol 25-OH-cholec lciferol 24, 25-diOH cholec lciferol 1, 25-diOH cholec lcifero l (B) Vit min B6 (D) Protein (B) Vit min D (D) Vit min K 265. This vit min
cts
s
nti-oxid nt:
255 Sucrose intoler nce le ds to (A) Hyper glycemi (D) Hypoglycemi
(C) Di rrhoe
(B) Glycosuri
256. There c n e intoler nce with respect to the following sug r: (A) Glucose ( C) M ltose (B) L ctose (D) Xylose 267. 1-hydroxyl tion of 25-OH vit min D3 t kes pl ce in (A) Liver (C) Intestine (B) Kidneys (D) P ncre s
126 268. 25-hydroxyl tion of vit min D3 t kes pl ce in (A) Liver (C) Intestine (B) K idneys (D) P ncre s MCQs IN BIOCHEMISTRY 279. This norm l met olite m y e responsi le for the neurologic l m nifest t ion of pernicious nemi : (A) T urine (B) Methyl m lonic cid (C) X ntherunic c id (D) Phenyl pyruvic cid 280. The vit min in le fy veget les: (A) D (C) A (B) K (D) Both (B) nd (C) 269. Hydroxyl tion of 25-hydroxy cholec lciferol is promoted (C) Cytochrome- (B) P r thyroid hormone (D) CAMP
y (A) Cytochrome -
270. The egg injury f ctor in r w egg white is (A) Biotin (C) Al umin (B) Avidin (D) C lcium s lts 281. Isonicotinic cid hydr zide given in the tre tment of tu erculosis m y le d to deficiency of (A) Vit min A (C) Fol te (B) Pyridoxin (D) Inositol 271. The following h s cy nide: (A) (B) (C) (D) Vit min B12 Adenyl co imid zole co mide Methyl co mide
mide Benz
282. Biotin is required for the re ction of CO2 with (A) (B) (C) (D) W ter Acety l CoA NH3 Incorpor tion of c r on 6 in purine 272. The hum n species c n B12 (D) Ni cin
iosynthesize (A) Vit min C (C) Thi mine (B) Vit min
283. A deficiency of fol te le ds to (A) (B) (C) (D) Meg lo l stic ic nemi Pernicious nemi Hypochromic microcytic nemi
nemi
Apl st
273. Retin cont ins this photosensitive pigment: (A) Rhodopsin (C) Retinol (B) Opsin (D) Mel nin 274. Anti xerophth lmic vit min is (A) Vit min B1 (C) Vit min B6 (B) Vit min B2 (D) Vit min A 284. A deficiency of Iron le ds to (A) (B) (C) (D) Meg lo l stic nemi Pernicious nemi Hypochromic microcytic nemi
nemi
Apl stic
275. One of the following is not symptom of ddison’s dise se. (A) Hypoglycemi (C) Hypok lemi (B) Hypon tremi (D) Hypochoremi 285. Corninoid coenzymes min B6 (D) Vit min B1 276. G mm x ne is (D) Inositol
n
re coenzymes of (A) Vit min B12 (C) Vit min B2 (B) Vit
ntimet
olite of (A) Thi mine (C) Pyridoxin (B) Ri ofl vin
286. Vit min B12 initi lly inds to the proteins known s (A) (B) (C) (D) Tr nsc o l min I R-Proteins Tr nsco l min II Intrinsic f ctor of c stle 277. Pyridoxin deficiency m y le d to convulsions sis of (A) GABA (C) EFA (B) PABA (D) SAM 278. Sulph drugs re oxin (D) Vit min B2
ntimet
s it is needed for the synthe
olities of (A) Vit min K (C) Folic
cid (B) Pyrid
287. Extrinsic f ctor of c stle is (A) Vit min B12 (C) R-Proteins (B) Glycoprote in (D) Sigm protein
VITAMINS 288. Intrinsic f ctor of c stle is (A) Vit min B12 (C) R-Proteins 289. Perniciou s me ns (A) Prolonged (C) Intermittent (B) D ngerous (D) Idiop thic (B) Glycopro tein (D) Sigm protein 127 297. Convulsive episodes occur when there is ne (C) Thi mine (B) Folic cid (D) Ri ofl vin
severe deficiency of (A) Pyridoxi
298. Met st tic cl ssific tion is seen in hypervit minosis: (A) A (C) D (B) K (D ) E 290. Reduction of D-ri onucleotides to Ddeoxy ri onucleotides in prok ryotes req uires (A) (B) (C) (D) 5, 6 dimethyl enzimid zole co mide Thioredoxin Tetr hyd ro iopterin Tetr hydrofol te 299. The nti vit min for p r mino enzoic mides (B) Dicoum rol (D) Thiop nic cid
cid is (A) Aminopterin (C) Sulphon
291. Biotin is lso known s (A) (B) (C) (D) Anti egg white injury f ctor Rutin Both (A) nd (B) None of these 300. Sever l p ntothenic cid deficiency in m n h s een reported to c use (A) ( B) (C) (D) Burning feet syndrome Scurvy C t r ct Xerophth lmi 292. Angul r stom tosis is due to (A) (B) (C) (D) Ari ofl vinoses Deficiency of Vit min C Deficiency of Vit min B1 Deficiency of fol te 301. Cholesterol is precursor in the (B) Vit min D (D) None of these
iogenesis of (A) Vit min A (C) Vit min E
302. This vit min is potent ntioxid nt of vit min A: (A) Vit min C (C) Vit mi n K (B) Vit min E (D) Vit min D 293. One of the m in functions of Vit min K is cof ctor for (A) C r oxyl te for the form tion of γ carboxy lutamate (B) Methylation of δ-a enosyl methionine (C) Car boxylation of biotin (D) One carbon transfer by tetrahy rofolate 294. Prothrombi n time is prolonge by a ministering (A) Vitamin K (C) Calcium (B) Dicoumarol (D ) Prothrombin 303. In retinal rickets, the following hy roxylation of Vitamin D3 place: (A) 25 (C) 24 (B) 1 (D) 7 304. The following ) COASH
oes not take
oes not have phosphorous: (A) Riboflavin (C) NAD+ (B) TPP (D
295. This vitamin acts as antioxi ant. (A) Vitamin A (C) Vitamin E (B) Vitamin D (D) Vitamin K 305. Convulsions an elirium coul be cause mine (C) Niacin (B) Glutamate (D) Magnesium
by a severe
eficiency of (A) Thia
296. This is a photo-labile vitamin. (A) Thiamine (C) Niacin (B) Riboflavin (D) Cholecalciferol 306. Rice polishings contain this vitamin: (A) Riboflavin (C) Thiamine (B) Niaci n (D) Vitamin B12
128 307. In beri beri there will be accumulation of _______ in bloo . (A) Aceto acet ic aci (B) β-OH utyric acid (C) Pyruvic acid (D) Methyl malonic acid 308. Sympto ms of pellagra are (A) (B) (C) (D) Dermatitis and diarrhea only Dermatitis and d ementia only Diarrhea, dermatitis and dementia Diarrhea and elements only
MCQs IN BIOCHEMISTRY 316. Taurinuria may e encountered in (A) Permicious anemia (B) Beri eri (C) Pel legra (D) Folate deficiency 317. The three vitamins hich are specially required for proper nerve functions are acid: (A) (B) (C) (D) Thiamine, niacin and ri of lavin Thiamine, folic acid, choline Thiamine, ri oflavin, patothenic acid Thiami ne, pyridoxin, vitamin B12 (B) Milk (D) Lemon 309. Pyridoxine deficiency leads to (A) (B) (C) (D) Megalo lastic anemia Aplasti c anemia Hypochromic microcytic anemia Permicious anemia 318. This is a rich source for vitamin C. (A) Rice (C) Egg 319. The follo ing vitamin is involved in coenzyme function in transaminations: (A) Nicotinamide (C) Thiamine (B) Pyridoxine (D) Ri oflavin 310. The significant ocular lesion in ar o flovinosis: (A) (B) (C) (D) Keratomal acia Bitot’s spots Vascularisation of the cornea lachrymal metaplasia 320. Methyl malonic aciduria is seen in the deficiency of (A) Vitamin B6 (C) Thi amine (A) Rickets (C) Night lindness (B) Folic acid (D) Vitamin B12 (B) Scurvy (D) All of these 311. Irradiation of foods raises the content of (A) Vitamin A (C) Vitamin E (B) Vitamin D (D) Vitamin K 321. Deficiency of Vitamin C leads to 312. An anti-vitamin for folic acid is (A) Amethoptesin (C) Pyrithoamine 313. Th ymine is (A) (B) (C) (D) Water solu le vitamin Fat solu le vitamin Purine ase P yrimidine ase (B) Dicoumarol (D) Isoniazid 322. If no primer DNA as given, the follo ing scientist could not have synthesi zed DNA. (A) Ochoa (C) Korn erg (A) Vitamin B1 (C) Vitamin E (B) Okazaki (D) Mon od (B) Vitamin B2 (D) Vitamin K 323. Antisterility vitamin is 314. The anti-vitamin for para amino ) Dicoumarol (D) Sulphonamides
enzoic acid is (A) Aminopterrin (C) INH (B
324. All the follo ing vitamins give rise to cofactors that are phosphorylated i n the active form except (A) Vitamin A (C) Vitamin D (A) (B) (C) (D) (B) Vitamin B1 (D) Vitamin E 315. The sulphur-containing vitamins among the follo ing B-Vitamin is (A) Thiami ne (C) Niacin (B) Ri oflavin (D) Pyridoxine 325. Molecular Iron, Fe, is Stored in the ody in com ination ith Ferritin Stor ed primarily in the spleen Excreted in the urine as Fe2+ a sor ed in the intesti ne y al umin
VITAMINS 326. Humans most easily tolerate a lack of hich of the follo ing nutrients? (A) Protein (C) Car ohydrate (B) Iodine (D) Lipid 129 335. Vitamins that function as dinucleotide derivatives include all the follo in g except (A) Thiamine (C) Nicotinate (B) Niacin (D) Vitamin B2 327. A deficiency of vitamin B12 causes (A) Cheliosis (B) Beri eri (C) Perniciou s anemia (D) Scurvy 328. In adults a severe deficiency of vitamin D causes (A) N ight lindness (C) Rickets (B) Osteomalacia (D) Skin cancer 336. Methyl malonic aciduria is seen in a deficiency of (A) Vitamin B6 (C) Thiam ine (B) Folic acid (D) Vitamin B12 337. What is the disease caused ts (B) Scurvy (D) Beri eri
y thiamine deficiency? (A) Nyctalopia (C) Ricke
329. Which of the follo ing vitamins ould most likely ecome deficient in a per son ho develops a completely carnivorous life style? (A) Thiamine (C) Co alamin e (B) Niacin (D) Vitamin C 338. Retinol and Retinol inding protein are ound n (C) α-glo ulin (B) Pre l umin (D) β-glo ulin
ith this protein: (A) Al umi
330. Which of the following st tements reg rding Vit min A is true? (A) (B) (C) (D) It is not n essenti l Vit min It is rel ted to tocopherol It is component of rhodopsin It is lso known s Opsin 339. Meg lo l stic nemi is c used (B) Vit min B6 (D) Protein
y the deficiency of (A) Folic
cid (C) Iron
340. This vit min cts s nti oxid nt. (A) Vit min A (C) Vit min E 341. C lcitr iol is (A) (B) (C) (D) 1-hydroxy cholec lciferol 25-hydroxy cholec lciferol 24, 25-dihydroxy cholec lciferol 1, 25-dihydroxy cholec lciferol (B) Vit min D (D) V it min K 331. Fully ctiv ted pyruv te c r oxyl se depends upon the presence of (A) (B) ( C) (D) M l te nd Ni cin Acetyl CoA nd iotin Acetyl CoA nd thi mine pyrophosp h te Ox lo cet te nd iotin 332. P ntothenic cid is constituent of coenzyme involved in (A) Acetyl tion ( B) Dec r oxyl tion (C) Dehydrogen tion (D) Oxid tion 333. Biotin is involved in which of the following types of re ctions? (A) Hydroxyl tion (C) Dec r oxyl tion (B) C r oxyl tion (D) De min tion 342. 1-hydroxyl tion of 25-hydroxy Vit min D3 t kes pl ce in (A) Liver (C) Intes tine (B) Kidneys (D) P ncre s 343. 25-hydroxyl tion of Vit min D3 t kes pl ce in (A) Liver (C) Intestines (B) Kidneys (D) P ncre s 334. Which of the following vit mins is the precurssor of CoA? (A) Ri ofl vin (C ) Thi mine (B) P ntothen te (D) Co mide 344. Hydroxyl tion of 25-hydroxy cholec lciferol is promoted (C) Cytochrome (B) P nthyroid hormone (D) cAMP
y (A) Cytochrome A
130 345. The egg injury f ctor in r w egg white is (A) Biotin (C) Al umin (A) (B) (C ) (D) (B) Avidin (D) C lcium s lts MCQs IN BIOCHEMISTRY 356. Isonicotinic cid hydr zide given in the tre tment of tu erculosis m y le d to deficiency of (A) Vit min A (C) Fol te (A) Vit min A (C) Vit min D (A) (B) (C) (D) (A) (B) (C) (D) (B) Pyridoxin (D) Inositol (B) Vit min C (D) None of th ese 346. The following h s cy nide: Vit min B12 Adenyl co de Methyl co mide
mide Benzimid zole co
mi
357. Steroid l prohormone is 347. The hum n species c n iosynthesize (A) Vit min C (C) Thi mine (A) Rhodopsi n (C) Retinol (B) Vit min B12 (D) Ni cin (B) Opsin (D) M l nin 358. A deficiency of fol te le ds to Meg lo l stic nemi Apl stic nemi Pernic ious nemi Hypochromic microcytic nemi Meg lo l stic nemi Apl stic nemi P ernicious nemi Hypochromic microcytic nemi (B) Vit min B12 (D) Vit min B1 348. Retin
cont ins this photo sensitive pigment.
359. Deficiency of Iron le ds to 349. Antixerophth lmic vit min is (A) Vit min B1 (C) Vit min B6 (B) Vit min B2 ( D) Vit min A 350. One of the following is not symptom of Addison’s dise se: (A) Hypoglycemi (C ) Hypok lemi (A) Thi mine (C) Pyridoxin (B) Hypon tremi (D) Hypochloremi (B) Ri ofl vin (D) Inositol 360. Corrinoid coenzymes 351. G mm xine is
n
re coenzymes of (A) Vit min B6 (C) Vit min B2
ntimet
olite of
361. Vit min B12 initi lly inds to the proteins known s (A) (B) (C) (D) Tr nsc o l min I R-proteins Tr nsco l min II Intrinsic f ctor of c stle (B) Glycoprot ein (D) Sigm protein (B) Glycoprotein (D) Sigm protein (B) D ngerous (D) Idiop thic 352. Pyridoxine deficiency m y le d to convulsions esis of (A) GABA (C) EFA (B) PABA (D) SAM
s it is needed for the synth
362. Extrinsic f ctor of c stle is (A) Vit min B12 (C) R-proteins (A) Vit min B1 2 (C) R-proteins 364. Pernicious me ns (A) Prolonged (C) Intermittent 353. Sulph drugs re D) P ntothenic cid
ntimet
olites of (A) PABA (C) Vit min B2 (B) Pyridoxin (
363. Intrinsic f ctor of c stle is 354. This norm l met olite m y e responsi le for the neurologic l m nifest t ion of pernicious nemi . (B) Methyl m lonic cid (A) T urine (C) X nthurenic c id (D) Phenyl pyruvic cid 355. Choline is not required for the form tion of (A) Lecithins (C) Sphingomyelin (B) Acetyl choline (D) Cholic cid 365. Reduction of D-ri onucleotides to D-deoxy ri onucleotides in prok ryotes re quires (A) (B) (C) (D) 5, 6 dimethyl enzimind zole co mide Thiredoxin Tetr hy
dro iopterin Tetr
hydrofol te
VITAMINS 366. Antir chitic vit min is (A) Vit min A (C) Vit min E (A) (B) (C) (D) (B) Vit min D (D) Vit min K 131 376. Cholesterol is precursor in the (B) Vit min D (D) None of these
iogenesis of (A) Vit min A (C) Vit min E
367. Angul r stom titis is due to Ari ofl vinosis Deficiency of Vit min C Defici ency of Vit min B1 Deficiency of fol te 377. Which of the vit mins is potent ntioxid nt of Vit min A? (A) Vit min C ( C) Vit min K (B) Vit min E (D) Vit min D 368. One of the m in functions of Vit min K is the cof ctor for (A) C r oxyl se for the form tion of γ carboxy lutamate (B) Methylation by S adenosyl methionine (C) Carboxylation by biotin (D) One carbon transfer by tetra hydrofolate 369. P rothrombin time is prolon ed by administerin (A) Vitamin K (C) Calcium (A) Vita min A (C) Vitamin E (A) Thiamine (C) Niacin (B) Dicoumarol (D) Prothrombin (B) V itamin D (D) Vitamin K (B) Riboflavin (D) Cholecalciferol 378. In renal rickets, the followin lace: (A) 25 (C) 24 (B) 1 (D) 7 379. Which of the followin B) TPP (D) CaASH
hydroxylation of Vitamin D3 does not take p
does not have phosphorous? (A) Riboflavin (C) NAD+ (
380. Rice polishin s contain whcih of the followin Thiamine (B) Niacin (D) Vitamin B12
Vitamin? (A) Riboflavin (C)
370. This Vitamin acts as antioxidant: 371. This is photo labile vitamin: 381. In beri beri there will be accumulation of _________ in blood. (A) (B) (C) (D) (A) (B) (C) (D) (A) (B) (C) (D) Aceto acetic acid β−hydroxy butyric acid Pyruvic acid Methyl malonic acid Dermatitis and diarrhea only Dermatitis and Dermentia only Diarrhea and dermentia only Diarrhea, Dermatitis and dementia Me aloblastic anemia Aplastic anemia Hypochromic microcytic anemia Pernicious anemia 372. Convulsive episodes occur when there is a severe deficiency of: (A) Pyridox ine (C) Thiamine (B) Folic acid (D) Riboflavin 382. Symptoms of pella ra are 373. Metastatic calcification is seen in hypervitaminosis: (A) A (C) D (B) K (D) E 383. Pyridoxine deficiency leads to 374. The anti vitamin for para amino benzoic acid is (A) Aminopterin (C) Sulphan omides (B) Dicoumasol (D) Thiopamic acid 375. Severe patothemic acid deficiency in man has been reported to cause (A) (B) (C) (D) Burnin feet syndrome Scurvy Cataract Xeropththalmia 384. The si nificant ocular lesion in a riboflavinosis is (A) (B) (C) (D) Kerato malacia Bitot’s spots Vascularisation of the cornea Lachrynal metaplasia
132 385. An anti vitamin for folic acid is (A) Aminopterin (C) Pyrithiamine 386. Thi amine is (A) (B) (C) (D) Water soluble vitamin Fat soluble vitamin Purine base P yrimidine base (B) Dicoumarol (D) Isoniazid MCQs IN BIOCHEMISTRY 395. Anti sterility Vitamin is (A) Vitamin B1 (C) Vitamin E (B) Vitamin B2 (D) V itamin K 396. Biotin deficiency is characterized by the followin except (A) Muscular pai n (C) Nausea (A) Beri beri (C) Ni ht blindness (A) Rickets (C) Xeropthalmia (A) A (C) C (A) Mitochondria (C) Lysosomes (B) Anaemia (D) Dermatitis (B) Scurvy (D) Rickets (B) Osteomalacia (D) Both (A) and (B) (B) B complex (D) E (B) Microtubu les (D) E.R 397. Deficiency of thiamine causes 387. The anti vitamin for para amino benzoic acid is (A) Aminopterin (C) INH (B) Dicoumarol (D) Sulphanomides 398. Deficiency of Vitamin D leads to 388. The sulphur containin vitamins amon the followin ne (C) Niacin (B) Riboflavin (D) Pyridoxine
B Vitamin is (A) Thiami
399. The vitamin that is useful in cancer is 389. Taurinuria may be encountered in (A) Pernicious anemia (B) Beriberi (C) Pel le ra (D) Folate deficiency 390. The three vitamins which are specially required for proper nerve functions are (A) (B) (C) (D) Thiamine, Niacin and Riboflavin Thiamin, Folic acid, Choline Thiamine, Riboflavin, Pantothenic acid Thiamine, Py ridoxin, Vitamin B12 (B) Milk (D) Lemon 400. Vitamin A over dosa e causes injury to 401. Which is a pro vitamin or vitamin that has antioxidant properties? (A) Beta carotene (C) Vitamin C (B) Vitamin E (D) Vitamin D 402. The vitamin required for carboxylation reaction is (A) Vitamin B2 (C) Bioti n (B) Vitamin B6 (D) Vitamin B12 391. This is a rich source for Vitamin C: (A) Rice (C) E 392. Which ot the followin vitamin is involved in coenzyme function in transami nations? (A) Nicotinamide (C) Thiamine (B) Pyridoxine (D) Riboflavin 403. Biolo ical activity of tocopherols has been attributed in part to their act ion as (A) (B) (C) (D) Antioxidant Anticoa ulents Provitamin Carriers in electro n transport system (B) Carboxylation (D) Transamination 393. Methyl malonic aciduria is seen in a deficiency of (A) Vitamin B6 (C) Thiam ine (B) Folic acid (D) Vitamin B12 404. Biotin is essential for (A) Translation (C) Hydroxylation 394. In pernicious anemia, Urine contains hi h amounts of (A) Methyl malonic aci d (B) FIGLU (C) VMA (D) 5 HIAA 405. Which of the followin 12 (B) Pyridoxine (D) C
vitamin act as a respiratory catalyst? (A) B2 (C) B
VITAMINS 406. Metal in Vitamin B12 is (A) Copper (C) Iron (B) Cobalt (D) Zinc 133 416. Durin deficiency of thiamine the concentration of the followin compound r ises in blood and intracellular fluid: (A) Glyco en (C) Amino acids (B) Su ar (D ) Pyruvic acid 407. Whole wheat is an excellent source of (A) Vitamin D (C) Vitamin A (B) Vitam in C (D) Thiamine 417. The conversion of carotenoids to Vitamin A takes place in (A) Intestine (C) Kidney (B) Liver (D) Skin 408. Vitamin used in the treatment of homocystinuria is (A) B1 (C) B 12 (B) B5 ( D) B6 418. Man cannot synthesize vitamin: (A) A (C) C (B) B (D) D 409. Which of the followin is not a component of coenzyme A? (A) Pantothenic ac id (C) Acetic acid (B) Adenylic acid (D) Sulfhydryl roup 419. Vitamin A is required for the formation of a li ht receptor protein known a s (A) Globulin (C) Chomoprotein (B) Lypoprotein (D) Rhodospin 410. The most active form of Vitamin D is (A) 25 Hydroxycholecalciferol (B) 1, 2 5 dihydroxycholecalciferol (C) 25 dihydroxyer ocalciferol (D) None of these 411. The important part in the structure of flavoprotein is (A) Vitamin B6 (C) Vitam in B1 (A) B1 (C) B6 (B) Vitamin B2 (D) Vitamin A 420. Excessive vitamin A in children produces (A) Irritability (C) Headache (B) Anorexia (D) All of these 421. Tocopherols prevent the oxidation of (A) Vitamin A (C) Vitamin K (B) Vitami n D (D) Vitamin C 412. Vitamin essential for transamination is (B) B2 (D) B 12 422. Vitamin K re ulates the synthesis of blood clottin factors. (A) VII (C) X (A) Pale yellow (C) Brown (B) IX (D) All of these (B) Pink (D) Bri ht red 423. The colour of cyanomethmo lobin is 413. The action of Vitamin K in formation of clottin factor is throu h (A) (B) (C) (D) Post transcription Post translation Gol i complex Endoplasmic reticulum 424. Transketolase activity is affected in (A) (B) (C) (D) Bitoin deficiency Pyr idoxine deficiency PABA deficiency Thiamine deficiency 414. Vitamin necessary for CoA synthesis: (A) Pantothenic acid (B) Vitamin C (D) B 12 (C) B6 415. Cofactor for transamination is (A) Thymine (C) Pyridoxine (B) Riboflavin (D) Niacin 425. The hydrolysis of lucose 6 PO4 is catalyzed by a phosphatase that is not f ound in which of the followin ? (A) Liver (C) Muscle (B) Kidney (D) Small intest ine
134 426. Vitamin K2 was ori inally isolated from (A) Soyabean (C) Alfa alfa (B) Putr id fishmeal (D) Oysters (A) Thiamine (C) Folic acid MCQs IN BIOCHEMISTRY (B) Riboflavine (D) Nicotininic acid 427. The followin form of vitamin A is used in the visual cycle: (A) Retinol (C ) Retinaldehyde (B) Retinoic acid (D) Retinyl acetate 430. The deficiency of which one of the followin vitamin causes creatinuria? (A ) Vitamin E (C) Vitamin A (B) Vitamin K (D) Vitamin B6 428. Increased carbohydrate consumption increases the dietary requirement for (A ) Thiamine (C) Pyridoxine (B) Riboflavine (D) Folic acid 431. A biochemical indication of vitamin B12 deficiency can be obtained by measu rin the urinary excretion of (A) (B) (C) (D) Pyruvic acid Malic acid Methyl mal onic acid Urocanic acid 429. Increased protein intake is accompanied by an increased dietary requirement for
VITAMINS 135 ANSWERS 1. A 7. D 13. A 19. A 25. C 31. D 37. C 43. A 49. B 55. D 61. B 67. B 73. C 79. C 85. C 91. A 97. C 103. D 109. A 115. A 121. C 127. A 133. D 139. C 145. C 151. C 157. A 163. C 169. A 175. D 181. D 187. D 193. C 199. B 205. A 211. C 217. D 223. B 229. B 235. B 241. B 2. B 8. A 14. D 20. A 26. A 32. A 38. B 44. A 50. A 56. B 62. B 68. C 74. B 80. D 86. A 92. A 98. D 104. C 110. D 116. D 122. A 128. A 134. C 140. B 146. D 152. D 158. D 164. A 170. B 176. B 182. D 188. C 194. D 200. C 206. C 212. A 218. C 224. D 230. C 236. C 242. B 3. A 9. D 15. B 21. B 27 . A 33. C 39. A 45. B 51. D 57. C 63. B 69. D 75. C 81. A 87. C 93. D 99. C 105. C 111. A 117. D 123. D 129. C 135. D 141. B 147. D 153. B 159. D 165. D 171. C 177. D 183. B 189. D 195. D 201. C 207. D 213. C 219. D 225. D 231. B 237. B 243 . C 4. A 10. A 16. C 22. D 28. A 34. C 40. D 46. C 52. C 58. A 64. B 70. A 76. A 82. D 88. A 94. A 100. B 106. B 112. D 118. C 124. D 130. D 136. D 142. B 148. B 154. D 160. D 166. C 172. B 178. B 184. A 190. C 196. C 202. D 208. D 214. D 2 20. D 226. C 232. A 238. D 244. C 5. A 11. B 17. A 23. A 29. C 35. B 41. D 47. A 53. C 59. A 65. A 71. A 77. A 83. C 89. D 95. A 101. B 107. C 113. B 119. C 125 . C 131. A 137. B 143. D 149. D 155. C 161. C 167. C 173. D 179. A 185. B 191. C 197. B 203. A 209. B 215. A 221. A 227. A 233. D 239. C 245. A 6. A 12. B 18. A 24. C 30. A 36. A 42. D 48. C 54. C 60. D 66.A 72. C 78. A 84. A 90. C 96. A 10 2. B 108. D 114. D 120. B 126. C 132. A 138. C 144. D 150. A 156. D 162. C 168. D 174. B 180. A 186. C 192. B 198. B 204. C 210. C 216. D 222. D 228. A 234. D 2 40. B 246. C
136 247. D 253. B 259. A 265. C 271. A 277. A 283. B 289. B 295. B 301. A 307. C 313 . D 319. B 325. A 331. B 337. D 343. A 349. D 355. D 361. B 367. A 373.C 379. A 385. A 391. D 397. A 403. B 409. C 415. C 421. A 427. C 248. A 254. D 260. B 266 . D 272. D 278. C 284. A 290. B 296. C 302. B 308. C 314. D 320. D 326. C 332. A 338. B 344. B 350. C 356. B 362. A 368. A 374. C 380. C 386. D 392. B 398. D 40 4. B 410. A 416. D 422. D 428. A 249. B 255. C 261. D 267. B 273. A 279. A 285. D 291. A 297. B 303. B 309. C 315. A 321. C 327. C 333. B 339. A 345. B 351. D 3 57. C 363. B 369. B 375. A 381. C 387. D 393. D 399. A 405. A 411. B 417. A 423. D 429. A 250. C 256. B 262. D 268. A 274. D 280. D 286. B 292. B 298. A 304. A 310. B 316. A 322. C 328. B 334. B 340. D 346. A 352. A 358. A 364. B 370. C 376 . B 382. D 388. A 394. A 400. C 406. B 412. C 418. C 424. D 430. C MCQs IN BIOCHEMISTRY 251. D 257. C 263. B 269. B 275. C 281. D 287. B 293. A 299. C 305. D 311. C 317 . D 323. C 329. D 335. A 341. D 347. D 353. A 359. D 365. A 371. B 377. B 383. C 389. A 395. C 401. B 407. D 413. B 419. D 425. C 431. C 252. C 258. C 264. A 27 0. B 276. D 282. B 288. A 294. A 300. C 306. D 312. A 318. D 324. B 330. C 336. D 342. B 348. A 354. B 360. B 366. B 372. A 378. B 384. C 390. D 396. B 402. C 4 08. D 414. A 420. D 426. B
VITAMINS 137 250. C The liver can store up to six years worth of vitamin B12, hence deficienc ies in this vitamin are rare. Penicious anemia is a me aloblastic anemia resulti n from vitamin B12 deficiency that develops as a result a lack of intrinsic fac tor in the stomach leadin to malabsorption of the vitamin. Biotin is also calle d anti e white injury factor because, e white contains a protein called avid in, which combines with biotin in the intestinal tract and prevents absorption o f biotin from intestines. Deficiency in Vitamin C leads to the disease scurvy du e to the role of the vitamin in the posttranslational modification of colla ens. Scurvy is characterized by easily bruised skin, muscle fati ue, soft swollen u ms, decreased wound healin and hemorra in , osteoporosis and anemia. Vitamin D is a steroid prohormone. It is represented by steroids that occur in animals, pl ants and yeast. Active form of the hormone is 1, 25 dihydroxy vitamin D3 (1, 25 (OH)2D3, also termed calcitriol). Calcitriol functions primarily to re ulate cal cium and phosphorous homeostasis. The main symptom of vitamin D deficiency in ch ildren is rickets and in adults is osteomalacia. Rickets is characterized by imp roper mineralization durin the development of the bones resultin in soft bones . Osteomalacia is characterized by demineralization of previously formed bone le adin to increased softness and susceptibility to fracture. EXPLANATIONS FOR THE ANSWERS 7. D The four fat soluble vitamins (A, D, E, K) are soluble in fats, oils and fa t solvents (alcohol, acetone etc.). Their occurrence in the diet, absorption and transport are associated with fat. All the fat soluble vitamins contain one or more of isoprene units (5 carbon units). They can be stored in liver and adipose tissue. Vitamin A is essential to maintain healthy epithelial tissues and prope r immunity. Retinol and retinoic acid functions like steroid hormones. They re u late protein synthesis and thus are involved in cell rowth and differentiation. βCarotene functions as an antioxidant and reduces the risk for heart attack, canc ers etc. The recommended dietary allo ances for vitamin D is around 400 I.U. In countries ith good sunlight (like India), it is much lo er. i.e., 200 I.U. The good sources include fatty fish, fish liver oils, egg yolk. The earliest symptom s of thiamin deficiency include constipation, appetite suppression, nausea as e ll as mental depression, peripheral neuropathy and fatigue. Chronic thiamin defi ciency leads to more severe neurological symptoms including ataxia, mental confu sion and loss of eye coordination. Other clinical symptoms of prolonged thiamin deficiency are related to cardiovascular and muscular defects. The severe thiami n deficiency disease is kno n as Beri eri. Ri oflavin deficiency is often seen i n chronic alcoholics due to their poor dia etic ha its. Symptoms associated ith ri oflavin deficiency include, glossitis, se orrhea, angular stomatitis, cheilo sis and photopho ia. Ri oflavin decomposes hen exposed to visi le light. Pyrido xal, pyridoxamine and pyridoxine are collectively kno n as vitamin B6. All three compounds are efficiently converted to the iologically active form of vitamin B6, pyridoxal phosphate. This conversion is catalyzed y the ATP requiring enzym e, pyridoxal kinase. Isoniazid (anti-tu erculosis drug) and penicillamine (used to treat rheumatoid arthritis and cystinurias) are t o drugs that complex ith p yridoxal and pyridoxal phosphate resulting in a deficiency in this vitamin. 291. A 40. D 321. B 77. A 357. C
110. D 398. D 149. D 187. D 217. D
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CHAPTER 6 ENZYMES 1. The compound hich has the lo est density is (A) Chylomicron (C) α-Lipoprotein (B) β-Lipoprotein (D) pre β-Lipoprotein 7. Kr e’s dise se is due to the deficiency of the enzyme: (A) Cer mide l ctosid se (B) Cer mid se (C) β-G l ctosid se (D) GM1 β-G l ctosid se 8. F ry’s dise se is du e to the deficiency of the enzyme: (A) Cer mide trihexosid se (B) G l ctocere ro sid se (C) Phyt nic cid oxid se (D) Sphingomyelin se 9. F r er’s dise se is due t o the deficiency of the enzyme: (A) α-G l ctosid se (B) Cer mid se (C) β-Glucocere r osid se (D) Arylsulph t se A. 10. A synthetic nucleotide n logue, used in org n tr nspl nt tion s suppressor of immunologic rejection of gr fts is (A) Theop hylline (B) Cyt r ine (C) 4-Hydroxypyr zolopyrimidine (D) 6-Merc ptopurine 2. Non steroid l nti infl mm tory drugs, such s spirin ct y inhi iting the ctivity of the enzyme: (A) Lipoxygen se (B) Cyclooxygen se (C) Phospholip se A2 (D) Lipoprotein lip se 3. From r chidon te, synthesis of prost gl ndins is c t lysed y (A) (B) (C) (D) Cyclooxygen se Lipoxygen se Throm ox ne synth se Isome r se (B) Apo enzyme (D) All of these 4. A Holoenzyme is (A) Function l unit (C) Coenzyme 5. G ucher’s dise se is due to the deficiency of the enzyme: (A) α-Fucosid se (C) β-Gl ucosid se (B) β-G l ctosid se (D) Sphingomyelin se 6. Neim nn-Pick dise se is due to the deficiency of the enzyme: (A) (B) (C) (D) Hexos minid se A nd B Cer mid se Cer mide l ctosid se Sphingomyelin se
140 11. Ex mple of n extr cellul r enzyme is (A) L ct te dehydrogen se (B) Cytochro me oxid se (C) P ncre tic lip se (D) Hexokin se 12. Enzymes, which re produced in in ctive form in the living cells, re c lled (A) P p in (C) Apoenzymes (B) L ysozymes (D) Proenzymes MCQs IN BIOCHEMISTRY 18. From the Linewe ver-Burk plot of Mich elis-Menten equ tion, Km nd Vm x c n e determined when V is the re ction velocity t su str te concentr tion S, the X- xis experiment l d t re expressed s (A) 1/V (C) 1/S (B) V (D) S 13. An ex mple of lig ses is (A) Succin te thiokin se (B) Al nine r cem se (C) F um r se (D) Aldol se 14 An ex mple of ly ses is (A) Glut mine synthet se (B) Fum r se (C) Cholinester se (D) Amyl se 15. Activ tion or in ctiv tion of cert in k ey regul tory enzymes is ccomplished y cov lent modific tion of the mino cid : (A) Tyrosine (C) Lysine (B) Phenyl l nine (D) Serine 19. A sigmoid l plot of su str te concentr tion ([S]) verses re ction velocity ( V) m y indic te (A) Mich elis-Menten kinetics (B) Co-oper tive inding (C) Compe titive inhi ition (D) Non-competitive inhi ition 20. The Km of the enzyme giving the kinetic d t s elow is (A) –0.50 (C) +0.25 (B) –0.25 (D) +0.33 21. The kinetic effect of purely competitive inhi itor of n enzyme (A) Incre se s Km without ffecting Vm x (B) Decre ses Km without ffecting Vm x (C) Incre se s Vm x without ffecting Km (D) Decre ses Vm x without ffecting Km 22. If curve X in the gr ph ( elow) represents no inhi ition for the re ction of the enzyme with its su str tes, the curve representing the competitive inhi ition, of the s me re ction is (A) A (C) C (B) B (D) D 16. The enzyme which c n dd w ter to c r on-c r on dou le ond or remove w te r to cre te dou le ond without re king the ond is (A) Hydr t se (C) Hydrol se (B) Hydroxyl se (D) Ester se 17. Fischer’s ‘lock nd key’ model of the enzyme ction implies th t (A) The ctive si te is complement ry in sh pe to th t of su st nce only fter inter ction. (B) Th e ctive site is complement ry in sh pe to th t of su st nce (C) Su str tes ch n ge conform tion prior to ctive site inter ction (D) The ctive site is flexi le nd djusts to su str te 23. An tutive nducer enzyme
inducer is sent in the type of enzyme: (A) Allosteric enzyme (B) Consti enzyme (C) Co-oper tive enzyme (D) Isoenzymic enzyme 24. A demonstr le i is sent in (A) Allosteric enzyme (B) Constitutive enzyme (C) Inhi ited (D) Co-oper tive enzyme
ENZYMES 25. In reversi le non-competitive enzyme ctivity inhi ition (A) (B) (C) (D) Vm x is incre sed Km is incre sed Km is decre sed Concentr tion of ctive enzyme is reduced 141 32. An enzyme c t lyzing oxidoreduction, using oxygen s hydrogen cceptor is (A ) (B) (C) (D) Cytochrome oxid se L ct te dehydrogen se M l te dehydrogen se Succ in te dehydrogen se 26. In reversi le non-competitive enzyme ctivity inhi ition (A) Inhi itor e rs structur l resem l nce to su str te (B) Inhi itor lowers the m ximum velocity tt in le with given mount of enzyme (C) Km is incre sed (D) Km is decre sed 27. In competitive enzyme ctivity inhi ition (A) The structure of inhi itor gen er lly resem les th t of the su str te (B) Inhi itor decre ses pp rent Km (C) K m rem ins un ffective (E) Inhi itor decre ses Vm x without ffecting Km 28. In e nzyme kinetics Vm x reflects (A) (B) (C) (D) The mount of n ctive enzyme Su s tr te concentr tion H lf the su str te concentr tion Enzyme su str te complex 33. The enzyme using some other su st nce, not oxygen s hydrogen cceptor is (A ) (B) (C) (D) Tyrosin se Succin te dehydrogen se Uric se Cytochrome oxid se 34. An enzyme which uses hydrogen cceptor s su str te is (A) (B) (C) (D) X nth ine oxid se Aldehyde oxid se C t l se Tryptoph n oxygen se 35. Enzyme involved in joining together two su str tes is (A) (B) (C) (D) Glut m ine synthet se Aldol se Gun ine de min se Argin se 36. The pH optim of most of the enzymes is (A) Between 2 nd 4 (B) Between 5 n d 9 (C) Between 8 nd 12(D) A ove 12 37. Coenzymes re (A) He t st le, di lyz le, non protein org nic molecules (B) Solu le, colloid l, protein molecules (C) Structur l n logue of enzymes (D) Different forms of enzymes 38. An ex mple of hydrogen tr nsferring coenzyme is (A) CoA (C) Biotin (B) NAD+ (D) TPP 29. In enzyme kinetics Km implies (A) The su str te concentr tion th t gives one h lf Vm x (B) The dissoc tion const nt for the enzyme su str te comples (C) Con centr tion of enzyme (D) H lf of the su str te concentr tion required to chieve Vm x 30. In competitive enzyme ctivity inhi ition (A) (B) (C) (D) App rent Km is decre sed App rent Km is incre sed Vm x is incre sed Vm x is decre sed 31. In non competitive enzyme ctivity inhi ition, inhi itor (A) Incre ses Km (B ) Decre ses Km (C) Does not effect Km (D) Incre ses Km 39. An ex mple of group tr nsferring coenzyme is (A) NAD+ (C) FAD (B) NADP+ (D) CoA
142 40. Coc r oxyl se is (A) (B) (C) (D) Thi mine pyrophosph te Pyridox l phosph te Biotin CoA MCQs IN BIOCHEMISTRY 48. The norm l serum GOT ctivity r nges from (A) 3.0–15.0 IU/L (C) 4.0–60.0 IU/L (B ) 4.0–17.0 IU/L (D) 0.9–4.0 IU/L 49. The norm l GPT ctivity r nges from (A) 60.0–250.0 IU/L (B) 4.0–17.0 IU/L (C) 3. 0–15.0 IU/L (D) 0.1–14.0 IU/L 50. The norm l serum cid phosph t se ctivity r nges from (A) (B) (C) (D) 5.0–13.0 KA units/100 ml 1.0–5.0 KA units/100 ml 13.0–18.0 KA uni ts/100 ml 0.2–0.8 KA units/100 ml 41. A coenzyme cont ining non ) Biotin
rom tic hetero ring is (A) ATP (C) FMN (B) NAD (D
42. A coenzyme cont ining rom tic hetero ring is (A) TPP (C) Coenzyme Q 43. Iso enzymes re (A) Chemic lly, immunologic lly nd electrophoretic lly different fo rms of n enzyme (B) Different forms of n enzyme simil r in ll properties (C) C t lysing different re ctions (D) H ving the s me qu tern ry structures like th e enzymes 44. Isoenzymes c n e ch r cterized y (A) Proteins l cking enzym tic ctivity th t re necess ry for the ctiv tion of enzymes (B) Proteolytic enzyme s ctiv ted y hydrolysis (C) Enzymes with identic l prim ry structure (D) Simil r enzymes th t c t lyse different re ction 45. The isoenzymes of LDH (A) Differ only in single mino cid (B) Differ in c t lytic ctivity (C) Exist in 5 for ms depending on M nd H monomer contents (D) Occur s monomers 46. The norm l v lue of CPK in serum v ries etween (A) 4–60 IU/L (C) 4–17 IU/L (B) 60–250 IU/L (D) > 3 50 IU/L (B) Lipoic cid (D) Biotin 51. The norm l serum lk line phosph t se ctivity r nges from (A) (B) (C) (D) 1 .0–5.0 KA units/100 ml 5.0–13.0 KA units/100 ml 0.8–2.3 KA units/100 ml 13.0–21.0 KA uni ts/100 ml 52. In e rly st ges of myoc rdi l ischemi the most sensitive indic tor is the m e surement of the ctivity of (A) CPK (C) SGOT (B) SGPT (D) LDH 53. Serum cid phosph t se level incre ses in (A) (B) (C) (D) Met st tic c rcino m of prost te Myoc rdi l inf rction Wilson’s dise se Liver dise ses 54. Serum C rcinom
lk line phosph t se level incre ses in (A) (B) (C) (D) Hypothyroidism of prost te Hyperp r thyroidism Myoc rdi l ischemi
55. Serum lip se level incre ses in (A) P get’s dise se (B) G ucher’s dise se (C) Ac ute p ncre titis (D) Di etes mellitus 56. Serum ferroxid se level decre ses in (A) G ucher’s dise se (B) Cirrhosis of liver (C) Acute p ncre titis (D) Wilson’s dis e se 47. F ctors ffecting enzyme (D) All of these
ctivity: (A) Concentr tion (C) Temper ture (B) pH
ENZYMES 57. The isoenzymes LDH5 is elev ted in (A) (B) (C) (D) Myoc rdi l inf rction Pep tic ulcer Liver dise se Infectious dise ses 143 65. The pH optim for s liv ry n lyse is (A) 6.6–6.8 (C) 7.9 (A) 4.0 (C) 7.9 (A) 5.0–7.0 (C) 5.4–6.0 (A) 1.0–2.0 (C) 5.8–6.2 (A) 1.0-2.0 (C) 5.0–7.0 (A) C ne sug r (C) L c tose (B) 2.0–7.5 (D) 8.6 (B) 7.1 (D) 8.6 (B) 5.8–6.2 (D) 8.6 (B) 5.2–6.0 (D) 5.4–6.0 (B) 5.4–6.0 (D) 5.8–6.2 (B) St rch (D) Ri ose 66. The pH optim
for p ncre tic
n lyse is
58. On the third d y of onset of cute myoc rdi l inf rction the enzyme elev ted is (A) Serum AST (C) Serum LDH (B) Serum CK (D) Serum ALT 67. The pH optim
for sucr se is
59. LDH1 nd LDH2 re elev ted in (A) (B) (C) (D) Myoc rdi l inf rction Liver di se se Kidney dise se Br in dise se 68. The pH optim
for m lt se is
69. The pH optim
for l ct se is
60. The CK isoenzymes present in c rdi c muscle is (A) BB MM nd MB (D) MB only 70. The su str te for
nd MB (C) BB only (B)
myl se is
61. In cute p ncre titis, the enzyme r ised in first five d ys is (A) (B) (C) ( D) Serum myl se Serum l ctic dehydrogen se Urin ry lip se Urin ry myl se 71. The ion which ctiv tes s liv ry (B) Bic r on te (D) Pot ssium
myl se
ctivity is (A) Chloride (C) Sodium
72. The p ncre tic myl se ctivity is incre sed in the presence of (A) Hydrochl oric cid (B) Bile s lts (C) Thiocy n te ions (D) C lcium ions 73. A c r ohydr t e which c n not e digested in hum n gut is (A) Cellulose (C) Glycogen (B) St rc h (D) M ltose 62. Acute p ncre titis is ch r cterised y (A) L ck of synthesis of zymogen enzy mes (B) Continuous rele se of zymogen enzymes into the gut (C) Prem ture ctiv t ion of zymogen enzymes (D) In ctiv tion of zymogen enzymes 63. An ex mple of fun ction l pl sm enzyme is (A) (B) (C) (D) Lipoprotein lip se Amyl se Aminotr nsfe r se L ct te dehydrogen se 74. The sug r sor ed y f cilit ted diffusion nd requiring N nsporter is (A) Glucose (C) G l ctose (B) Fructose (D) Ri ose
independent tr
64. A non-function l pl sm enzyme is (A) (B) (C) (D) Psudocholinester se Lipopr otein lip se Proenzyme of lood co gul tion Lip se 75. In the intestine the r te of sorption is highest for (A) (B) (C) (D) Gluco se nd g l ctose Fructose nd m nnose Fructose nd pentose M nnose nd pentose
144 76. Glucose ) Vit min K
sorption is promoted
y (A) Vit min A (C) Vit min C (B) Thi min (D
MCQs IN BIOCHEMISTRY 84. In the glycolytic p thw y, enolpyruv te is converted to ketopyruv te y (A) Pyruv te kin se (B) Phosphoenolpyruv te (C) Pyruv te dehydrogen se (D) Spont neo usly 85. In erythrocytes, 2, 3- iphosphoglycer te is derived from the intermedi te: (A) Glyer ldehyde-3-phosph te (B) 1, 3-Biphosphoglycer te (C) 3-Phosphoglyce r te (D) 2-Phosphoglycer te 86. 2, 3-Biphosphoglycer te in high concentr tions, com ines with hemoglo in, c uses (A) Displ cement of the oxyhemoglo in dissoci t ion curve to the left (B) Displ cement of the oxyhemoglo in dissoci tion curve t o the right (C) No ch nge in oxy hemoglo in dissoci tion curve (D) Incre sed ff inity for oxygen 87. Erythrocytes under norm l conditions nd microorg nisms und er n ero ic conditions m y ccumul te (A) (B) (C) (D) NADPH Pyruv te Phosphoeno lpyruv te L ct te 77. The h rmone cting directly on intestin l mucos nd stimul ting glucose orption is (A) Insulin (C) Thyroxine (B) Gluc gon (D) V sopressin
s
78. Given th t the st nd rd free energy ch nge (∆G°) for the hydrolysis of ATP is ∆ –7.3 K c l/mol nd th t for the hydrolysis of Glucose 6-phosph te is –3.3 Kc l/mol, th e ∆ G° for the phosphoryl tion of glucose is Glucose + ATP → Glucose 6– Phosph te + ADP. (A) –10.6 Kc l/mol (C) –4.0 Kc l/mol (B) –7.3 Kc l/mol (D) +4.0 Kc l/mol 79. At low lood glucose concentr tion, r in ut not liver will t ke up glucose . It is due to the (A) (B) (C) (D) Low Km of hexokin se Low Km of glucokin se Sp ecificity of glucokin se Blood r in rrier 80. In the re ction elow, Nu TP st nds for NuTP + glucose → Glucose 6–Phosph te + N uDP. (A) ATP (C) GTP (B) CTP (D) UTP 81. In the figures shown A (C) C (B) B (D) D
elow, fructose 1,6 iphosph te is loc ted
t point: (A)
88. Enzymes le ding to the high energy phosphoryl tion of su str tes during glyc olysis include which of the following? (A) Phosphoglycer te kin se (B) Enol se ( C) Pyruv te Kin se (D) Glycer ldehyde-3-phosph te dehydrogen se 89. Linewe ver – B urk dou le reciproc l plot is rel ted to (A) Su str te concentr tion (B) Enzyme ctivity (C) Temper ture (D) Both (A) nd (B) 82. The enzyme of the glycolic p thw y, sensitive to inhi iton is (A) Hexokin se (C) Enol se (B) Aldol se (D) Pyruv te kin se
y fluoride ions
83. In glycolytic p thw y, iod cet te inhi its the ctivity of the enzyme: (A) ( B) (C) (D) Phosphotriose isomer se Glycer ldehyde-3-phosph te dehydrogen se Pyru v te kin se Phosphofructokin se
ENZYMES 90. Phosphofructokin se key enzyme in glycolysis is inhi ited ATP (C) ADP (B) AMP (D) TMP 145 97. Pyruv te dehydrogen se Zinc (D) Sodium
ctivity is inhi ited
y (A) Citr te
nd
y (A) Mercury (C) C lcium (B)
91. One of the enzymes regul ting glycolysis is (A) Phosphofructokin se (B) Glyc er ldehyde-3-phosph te dehydrogen se (C) Phosphotriose isomer se (D) Phosphohexo se isomer se 92. Hexokin se is inhi ited in n llosteric m nner y (A) Glucose6-Phosph te (B) Glucose-1-Phosph te (C) Fructose-6-phosph te (D) Fructose-1, 6iphosph te 93. A re ction which m y e considered n isomeris tion is (A) Glucos e 6-Phosph te (B) 3-Phosphoglycer te (C) 2-phosphoglycer te pyruv te (D) Pyruv t e L ct te fructose 6 phosph te 2-phosphoglycer te phosphoenol98. In the norm l resting st te of hum ns, most of the lood glucose uel is consumed y (A) Liver (C) Muscle (B) Adipose tissue (D) Br in
urned
s f
99. All the enzymes of glycolysis p thw y re found in (A) (B) (C) (D) Extr mito chondri l solu le fr ction of the cell Mitochondri Nucleus Endopl smic reticulu m 100. Most m jor met olic p thw ys re considered m inly either n olic or c t olic. Which of the following p thw y is most correctly considered to e mphi o lic? (A) Citric cid cycle (C) Lipolysis (B) Gluconeogenesis (D) Glycolysis 101. The enzymes of the citric cid cycle re loc ted in (A) (B) (C) (D) (A) (B) (C) (D) Mitochondri l m trix Extr mitochondri l solu le fr ction of the cell Nu cleus Endopl smic reticulum Conversion of pyruv te to cetyl-CoA Condens tion of cetyl-CoA with ox lo cet te Conversion of citr te to isocitr te Form tion of α ketoglut r te c t lysed y isocitr te dehydrogen se 94. The net num er of ATP formed per mole of glucose in (A) 1 (C) 6 (B) 2 (D) 8 102. The initi l step of the citric
n ero ic glycolysis is
cid cycle is
95. Pyruv te dehydrogen se multienzyme complex is required for the production of (A) Acetyl-CoA (B) L ct te (C) Phosphoenolpyruv te (D) Enolpyruv te 96. Diet ry deficiency of thi min inhi its the ctivity of the enzyme: (A) Pyruv te kin s e (B) Pyruv te dehydrogen se (C) Phosphofructokin se (D) Enol se 103. The su st nce which m y e considered to pl y c t lytic role in citric id cycle is (A) Ox lo cet te (C) M l te (B) Isocitr te (D) Fum r te
c
104. An enzyme of the citric cid cycle lso found outside the mitochondri is ( A) (B) (C) (D) Isocitr te dehydrogen se Citr te synthet se α-Ketoglut r te dehydro gen se M l te dehydrogen se
146 105. The re ction c t lysed y α-ketoglut r te dehydrogen se in the citric cle requires (A) NAD (C) ADP (B) NADP (D) ATP MCQs IN BIOCHEMISTRY 111. Form tion of succinyl-CoA from α-Ketoglut r te is inhi ited te (C) Fluoride (B) Arsenite (D) Iodo cet te
cid cy
y (A) Fluoro cet
106. If ll the enzymes, intermedi tes nd cof ctors of the citric cid cycle s well s n excess of the st rting su str te cetylCoA re present nd function l in n org nelle free solution t the ppropri te pH, which of the following f ctors of the citric cid cycle would prove to e r te limiting? (A) (B) (C) (D) Molecul r oxygen H lf life of enzyme Turnover of intermedi tes Reduction of cof ctors 112. The num er of ATP molecules gener ted for e ch turn of the citric e is (A) 8 (C) 24 (B) 12 (D) 38
cid cycl
113. Oxid tion of one molecule of glucose yields (A) 12 ATP (C) 38 ATP (B) 24 AT P (D) 38 ATP 114. Which of the following intermedi tes of met olism c n e oth precursor nd product of glucose? (A) L ct te (C) Al nine (B) Pyruv te (D) Acetyl-CoA 107. In TCA cycle, ox losuccin te is converted to α -ketoglut r te y the enzyme: (A) (B) (C) (D) Fum r se Isocitr te dehydrogen se Aconit se Succin se 115. Mitochondri l mem r ne is freely pre m le to (A) Pyruv te (C) Ox lo cet te (B) M l te (D) Fum r te 108. The enzyme -ketoglut r te dehydrogen se in the citric A) Lipo te (C) Pyridoxine (B) Fol te (D) Inositol
cid cycle requires (
116. The re ction of Kre ’s cycle which does not require cof ctor of vit min B gro up is (A) Citr te (B) isocitr te succin te 109. The ex mple of gener tion of high energy phosph te t the su str te level in the citric cid cycle is the re ction: (A) Isocitr te (B) Succin te (C) M l te α-Ketoglut r te α-fum r te α-ox lo cet te -Succin te α -Ketoglut r te (C) M l te (D) Succin te ox lo cet te fum r te 117. The coenzyme not involved in the form tion of A) TPP (C) NAD (B) Biotin (D) FAD
cetyl-CoA from pyruv te is (
(D) Succinyl CoA 110. Fluoro cet te inhi its the re ction of citric cid cycle: (A) Isocitr te (B ) Fum r te (C) Citr te (D) Succin te -Ketoglut r te α-M l te α-cis- conit te α-fum r te 118. A c rrier molecule in the citric te (B) Citr te (D) M l te
cid cycle is (A) Acetyl-CoA (C) Ox lo cet
119. A specific inhi itor for succin te dehydrogen se is (A) Arsenine (C) Citr t e (B) Arsenite (D) Fluoride
ENZYMES 120. The r te of citric cid cycle is controlled y the llosteric enzyme: (A) ( B) (C) (D) Aconit se Fum r se Fum r se M l te dehydrogen se 147 127. In glycogenolysis, the enzyme which tr nsfers tris cch ride unit from one → r nch to the other exposing 1→ 6 r nch point is (A) Phosphoryl se (B) α-[1→ 4]→ α-[1→ luc n tr nsfer se (C) Amylo [1→ 6] glucosid se (D) Amylo[1→ 4]→ [1→ 6] tr nsglucosid se 128. In the synthesis of glycogen from glucose the reversi le step is (A) Glucos e → glucose 6-phosph te (B) Glucose 6-phosph te → glucose 1-phosph te (C) Glucose 1phosph te → UDP glucose (D) UDP glucose → glycogen 129. The enzyme glucose-6-phosph t se which c t lyses the conversion of glucose 6-phosph te to glucose is not fou nd in (A) Liver (C) Intestine (B) Muscle (D) Kidney 121. In the erythrocytes, the net production of ATP molecules ering p thw y is (A) 0 (C) 4 (B) 2 (D) 8
y the R pport-Leu
122. The r tio th t most closely pproxim tes the num er of net molecules of ATP formed per mole of glucose utilized under ero ic conditions to the net num er formed under n ero ic conditions is (A) 4:1 (C) 18:1 (B) 13:1 (D) 24:1 123. The p thw y of glycogen iosynthesis involves speci l nucleotide of gluco se. In the re ction elow, NuDP st nds for NuDP Glucose + glycogenn → NuDP + glyco genn+1 (A) ADP (C) UDP (B) GDP (D) CDP 130. Allosteric ctiv tor of glycogen synth se is (A) Glucose (C) UTP (B) Glucos e-6-Phosph te (D) Glucose-1-phosph te 124. Glucose 6-phosph te is converted to glucose 1-phosph te in re ction c t l ysed y the enzyme phosphoglucomut se, which is (A) (B) (C) (D) Phosphoryl ted D ephosphoryl ted Phosphoryl ted-dephosphoryl ted Phosphoryl ted-dephosphoryl tedr ephosphoryl ted 131. Action of glycogen synth se is inhi ited se (D) Cyclic AMP
y (A) Insulin (C) Mg 2+ (B) Gluco
132. The hormone ctiv ting the glycogen synth se inephrine (B) Gluc gon (D) ACTH
ctivity is (A) Insulin (C) Ep
133. Ch r cteristic fe tures of ctive site re (A) Flexi le in n ture (B) Site of inding (C) Acidic (D) Both (A) nd (B) 134. Von Gierke’s dise se is ch r cteri zed y the deficiency of (A) Glucose-6-phosph t se (B) α -1 → 4 Glucosid se 125. The glycogen content of the liver is upto (A) 6% (C) 10% (B) 8% (D) 12% 126. In glycogenesis r nch point in the molecule is est lished y the enzyme (A) (B) (C) (D) Amylo[1→ 4][1→ 6] tr nsglucosid se α [1→ 4] α [1→ 4] Gluc n tr nsfer se A lo [1→ 6] glucosid se Glycogen synth se (C) 1 → 6 Glucosid se (D) Liver phosphoryl se
148 135. Cori dise se (Limit dextrinosis) is c used due to sence of (A) (B) (C) (D ) Br nching enzyme De r nching enzyme Glycogen synth se Phosphoryl se MCQs IN BIOCHEMISTRY 142. The hydrogen cceptor used in pentose phosph te p thw y is (A) NAD (C) FAD (B) NADP (D) FMN 143. The enzymes of the pentose phosph te p B) Mitochondri (C) Nucleus (D) Endopl smic p thw y, D-ri ulose5-phosph te is converted : (A) Fum r se (C) G-6-PD (B) Ketoisomer se
thw y re found in the (A) Cytosol ( reticulum 144. In pentose phosph te to D-ri ose-5phosph te y the enzyme (D) Epimer se
136. Mc Ardle’s syndrome is ch r cterized y the sence of (A) (B) (C) (D) Liver phosphoryl se Muscle phosphoryl se Br nching enzyme De r nching enzyme 137. Pompe’s dise se is c used due to deficiency of (A) (B) (C) (D) Lysosom l α-1→4 d 1→6-glucosid se Glucose-6-phosph t se Glycogen synth se Phosphofructokin se 138. Amylopectinosis is c used due to sence of (A) (B) (C) (D) De r nching enz yme Br nching enzyme Acid m lt se Glucose-6-phosph t se 145. The tr nsketol se enzyme in the pentose phosph te p thw y requires the B vi t min. (A) P ntothenic cid (C) Ri ofl vin (B) Thi min (D) Nicotinic cid 146. Xylulose-5-phosph te serves s don r of ctive glycol ldehyde, the ccept or is (A) Erythrose 4-phosph te (B) Ri ose 5-phosph te (C) Glycer ldehyde 3-phos ph te (D) Sedoheptulose 7-phosph te 147. Pentose phosph te p thw y is of signifi c nce ec use it gener tes (A) NADPH for reductive synthesis (B) Regener tes glu cose 6-phosph te (C) Gener tes fructose 6-phosph te (D) Forms glycer ldehyde 3-p hosph te 148. The pentose phosph te p thw y protects erythrocytes g inst hemoly sis y ssisting the enzyme: (A) Superoxide dismut se (B) C t l se (C) Glut thio nic peroxid se (D) Cytochrome oxid se 139. Her’s dise se is ch r cterized y deficiency of (A) (B) (C) (D) Muscle phosph oryl se Liver phosphoryl se De r nching enzyme Glycogen synth se 140. T rui dise se is ch r cterized y the deficiency of the enzyme: (A) (B) (C) (D) Liver phosphoryl se Muscle phosphoryl se Muscle nd erythrocyte phosphofruc tokin se Lysosom l cid m lt se 141. The hexose monophosph te p thw y includes the enzyme: (A) (B) (C) (D) M lt se dehydrogen se Hexokin se α-Ketoglut r te dehydrogen se Glucose-6-phosph te dehy drogen se
n
ENZYMES 149. Hemolytic nemi is c used y the deficiency of cert in enzymes of the pent ose phosph te p thw y, the princip l enzyme involved is (A) (B) (C) (D) (A) (B) (C) (D) (A) (B) (C) (D) Glucose-6-phosph te dehydrogen se Aldol se Fructose 1, 6 - isphosph t se Phosphohexose isomer se Liver nd kidney Skin nd p ncre s Lung nd r in Intestine nd lens of eye Pyruv te kin se Pyruv te c r oxyl se Hexokin se Phosphohexose isomer se 149 157. For conjug tion with m ny enogenous nd exogenous su st nces efore elimin tion in urine, the uronic cid p thw y provides (A) Active glucuron te (B) Gulon te (C) Xylulose (D) Xylitol 158. UDP glucose is converted to UDP glucurron te, re ction c t lysed y UDP glucose dehydrogen se requires (A) NAD+ (C) NADP (B) FAD (D) FMN 150. The sites for gluconeogenesis
re
151. An enzyme involved in gluconeogenesis is 159. Pentosuri is r re heredit ry dise se is ch r cterized y incre sed urin ry excretion of (A) (B) (C) (D) L-xylulose Xylitol Xylulose 5-phosph te Ri ose 5 -phosph te 152. The enzyme pyruv te c r oxyl se is present in (A) Cytosol (C) Nucleus (B) M itochondri (D) Golgi odies 160. The enzyme involved in essenti l pentosuri (B) Hydroxyl se (D) R cem se
is (A) Reduct se (C) Isomer se
153. The enzyme phosphoenolpyruv te c r oxykin se c t lyses the conversion of ox lo cet te to phosphoenolpyruv te requires (A) ATP (C) AMP (B) ADP (D) GTP 161. G l ctose is synthesized from glucose in (A) M mm ry gl nd (B) Intestine (C ) Kidney (D) Adipose tissue 162. G l ctose is re dily converted to glucose in (A ) Liver (C) Kidney (B) Intestine (D) Adipose tissue 154. The enzyme glucose 6-phosph t se is present in (A) Liver (C) Adipose tissue (B) Muscle (D) Br in 163. G l ctose 1-phosph te is converted to uridine diphosph te g l ctose, the re ction is c t lysed y the enzyme: (A) (B) (C) (D) Gl ctokin se G l ctose 1-phos ph te uridyl tr nsfer se Uridine diphospho g l ctose 4-epimer se UDP glucose pyr ophosphoryl se 155. In gluconeogensis, n llosteric ctiv tor required in the synthesis of ox lo cet te from ic r on te nd pyruv te, which is c t lysed y the enzyme pyruv te c r oxyl se is (A) Acetyl CoA (C) Isocitr te (B) Succin te (D) Citr te 164. The est known c use of g l ctosemi is the deficiency of (A) (B) (C) (D) G l ctose 1-phosph te nd uridyl tr nsfer se Phosphoglucomut se G l ctokin se L c tose synth se 156. The num er of ATP molecules required to convert 2 molecules of l ct te into glucose in m mm li n liver is (A) 2 (C) 5 (B) 4 (D) 6
150 165 Conversion of fructose to sor itol is c t lysed y the enzyme: (A) (B) (C) ( D) Sor itol dehydrogen se Aldose reduct se Fructokin se Hexokin se 172. Phlorizi n inhi its MCQs IN BIOCHEMISTRY (A) Ren l tu ul r re sorption of glucose (B) Glycolysis (C) Gluconeogenesis (D) Glycogenolysis 173. Ren l glycosuri is ch r cterized y (A) Hyperglycemi (B) Hyperglycemi with glycosuri (C) Norm l lood glucose level with glycosuri (D) Hyperglycemi with ketosis 174. Acute hemolytic nemi in person’s sensitive to t he F v e ns is due to the deficiency of the enzyme: (A) Pyruv te dehydrogen se (B) G-6-PD (C) Aconit se (D) Tr nsketol se 175 Acute hemolytic episode fter d ministr tion of ntim l ri l, prim quin, is due to deficiency of the enzyme: (A) 6-Phosphoglucon te dehydrogen se (B) Glucose-6-phosph te dehydrogen se (C) Epim er se (D) Tr nsketol se 176. The pH optim of g stric lip se is (A) 3.0–6.0 (C) 8. 0 (B) 1.0–2.0 (D) 8.6 166. A specific fructokin se present in liver h s very high ffinity for its s u str te ec use (A) (B) (C) (D) Km for fructose is very high Km for fructose is very low Activity is ffected y f sting Activity is ffected y insulin 167. Insulin h s no effect on the ctivity of the enzyme: (A) (B) (C) (D) Glycog en synthet se Fructokin se Pyruv te kin se Pyruv te dehydrogen se 168. The p thogenesis of di etic c t r ct involves e (C) Sor itol (B) M nnitol (D) Pyruv te
ccumul tion of (A) G l ctos
169. Heredit ry fructose intoler nce involves the sence of the enzyme: (A) (B) (C) (D) Ald lose B Fructokin se Triokin se Phosphotriose isomer se 170. Essenti l fructosuri is ch r cterized y the l ck of the hep tic enzyme: ( A) (B) (C) (D) Phosphohexose isomer se Ald lose A Aldol se B Fructokin se 177. The optimum pH of p ncre tic lip se is (A) 2.0 (C) 6.0 (B) 4.0 (D) 8.0 178. G stric lip e is + (B) Cu ++ (D) N
ctiv ted in the presence of (A) Bile s lts (C) K
+
171. In norm l individu ls glycosuri occurs when the venous lood glucose conce ntr tion exceeds (A) (B) (C) (D) 5–6 mmol/L 7–8 mmol/L 8.5–9 mmol/L 9.5–10 mmol/L 179. An ex mple of enzyme inhi ition: (A) Reversi le inhi ition (B) Irreversi le inhi ition (C) Allosteric inhi ition (D) All of these
ENZYMES 180. The form tion of ∆2-tr ns-enoyl-CoA from cyl-CoA requires the enzyme: (A) (B ) (C) (D) Acyl-CoA synthet se Acyl-CoA dehydrogen se 3-Hydroxy cyl-CoA dehydrog en se Thiol se 151 189. The concentr tion of ketone odies in the lood does not norm lly exceed (A ) 0.2 mmol/L (C) 1 mmol/L (B) 0.4 mmol/L (D) 2 mmol/L 181. In β-oxid tion 3-keto cyl-CoA is splitted t the 2, 3 position (A) Hydr t se (C) Reduc tse (B) Dehydrogen se (D) Thiol se
y the enzyme:
190. In hum ns under norm l conditions loss of ketone odies vi urine is usu ll y less th n (A) 1 mg/24 hr (C) 8 mg/24 hr (B) 4 mg/24 hr (D) 10 mg/24 hr 182. F tty cids with odd num er of c r on toms yield cetyl-CoA nd molecule of (A) Succinyl-CoA (C) M lonyl-CoA (B) Propionyl-CoA (D) Aceto cetyl-CoA 191. The structure which ppe rs to e the only org n to dd signific nt qu ntit ies of ketone odies to the lood is (A) Br in (C) Liver (B) Erythrocytes (D) Sk elet l muscle 183 For e ch of the first 7- cetyl-CoA molecules formed y α -oxid tion of p lmiti c cid, the yield of high energy phosph tes is (A) 12 (C) 30 (B) 24 (D) 35 192. The st rting eto cetyl-CoA (D) re ssoci ted m um Nucleus Golgi
m teri l for ketogenesis is (A) Acyl-CoA (B) Acetyl-CoA (C) Ac M lonyl-CoA 193. Enzymes responsi le for ketone ody form tion inly with the (A) (B) (C) (D) Mitochondri Endopl smic reticul pp r tus
184. The net g in of ATP/mol of p lmitic ) 129 (B) 105 (D) 135
cid on complete oxid tion is (A) 88 (C
185. ω-oxidation is normally a very minor path ay and is rought zymes involving (A) Cytochrome a (C) Cytochrome c (B) Cytochrome e p-450
y hydroxylase en (D) Cytochrom
194. The synthesis of 3-hydroxy-3-methylglutaryl-CoA can occur (A) (B) (C) (D) O nly in mitochondria of all mammalian tissues Only in the cytosol of all mammalia n tissue In oth cytosol and mitochondria In lysosomes 186. α -Oxid tion i.e., the remov l of one c r on t time from the c r oxyl end of the molecule h s een detected in (A) Br in tissue (C) Adipose tissue (B) Liv er (D) Intestine 195. In the p thw y le ding to iosynthesis of ceto cet te from cetyl-CoA in l iver, the immedi te precursor of ceot cet te is (A) (B) (C) (D) (A) (B) (C) (D) Aceto cetyl-CoA 3-Hydroxy utyryl-CoA 3-Hydroxy-3-methyl-glut ryl-CoA 3-Hydroxy utyr te Extr hep tic tissues Hep tic tissues Erythrocytes Mitochondri 187. In β-oxid tion, the coenzyme for B) NAD (D) FAD 196. Ketone
odies serve
s
cyl-CoA dehydrogen se is (A) FMN (C) NADP (
fuel for
188. The coenzyme involved in dehydrogen tion of 3-hydroxy C) NAD (B) FMN (D) NADP
cyl-CoA is (A) FAD (
152 197. In extr hep tic tissues, one mech nism for utiliz tion of ceto cet te inv olves (A) M lonyl-CoA (C) Propionyl-CoA 198. Ketosis reflects (A) (B) (C) (D) In cre sed hep tic glucose li er tion Incre sed f tty cid oxid tion Incre sed c r ohydr te utilis tion Incresed gluconeogenesis (B) Succinyl-CoA (D) Acetyl-CoA MCQs IN BIOCHEMISTRY 205. The f tty cid synth se complex c t lyses (A) (B) (C) (D) 4 sequenti l enzy m tic steps 6 sequenti l enzym tic steps 7 sequenti l enzym tic steps 8 sequenti l enzym tic steps 206. The m in source of reducing equiv lents (NADPH) for lipogenesis is (A) (B) (C) (D) Pentose phosph te p thw y Citric cid cycle Glycolysis Glycogenolysis 199. Ketosis is ssoci ted with the dise se: (A) (B) (C) (D) Nephritis Di mellitus Edem Coron ry rtery dise ses
etes
207. In f tty cids synth se of oth cteri nd m mm ls, ACP ( cyl c rrier pro tein) cont in the vit min: (A) Thi min (C) Ri ofl vin (B) Pyridoxine (D) P ntoth enic cid 200. The m in p thw y for denovo synthesis of f tty C) Microsomes (B) Mitochondri (D) Nucleus
cids occur in (A) Cytosol (
208. C r oxyl tion of cetyl-CoA to m lonylCoA requires the enzyme: (A) (B) (C) (D) Acetyl-CoA c r oxyl se Pyruv te c r oxyl se Acetyl tr ns cyl se Acyl CoA-syn thet se 201. Ch in elong tion of f tty cids in m mm li n liver occurs in (A) Nucleus (C ) Lysosomes (B) Ri osomes (D) Microsomes 209. The r te limiting re ction in the lipogenic p thw y is (A) (B) (C) (D) Acet yl-CoA c r oxyl se step Keto cyl synth se step Keto cyl reduct se step Hydr t se step 202. Acetyl-CoA is the princip l uilding lock of f tty cids. It is produced w ithin the mitochondri nd does not diffuse re dily into cytosol. The v il ili ty of cetyl CoA involves (A) (B) (C) (D) C rnitine cyl tr nsfer se Pyruv te de hydrogen se Citr te ly se Thiol se 210. Conversion of f tty cyl-CoA to n cylCoA deriv tive h ving 2 more c r on toms involves s cetyl don r: (A) Acetyl-CoA (C) Propionyl-CoA (B) Succinyl-Co A (D) M lonyl-CoA 203. The synthesis of f tty cids is often termed reductive synthesis. (A) NADP+ (C) FADH2 (B) NADH (D) NADPH 211. A cof ctor required for the conversion of cetyl-CoA to m lonyl-CoA in extr mitochondri l f tty cid synthesis is (A) Biotin (C) NAD (B) FMN (D) NADP 204. The protein, which is in f ct multifunction l enzyme complex in higher or g nism is (A) (B) (C) (D) Acetyl tr ns cyl se M lonyl tr ns cyl se 3-Hydroxy cy l-ACP dehyr t se F tty cid synth se 212. The glycerol for f tty cid esterific tion in dipocytes is (A) For the mos t p rt, derived from glucose (B) O t ined prim rily from phosphoryl tion of glyc erol y glycerol kin se (C) Formed from gluconeogenesis (D) Formed from glycogen olysis
ENZYMES 213. In the iosynthesis of triglycerides from glycerol 3-phosph te nd cyl-CoA , the first intermedi te formed is (A) (B) (C) (D) 2-Mono cylglycerol 1, 2-Di cy lglycerol Lysophosph tidic cid Phosph tidic cid 153 221. Synthesis of phosph tidylinositol y tr nsfer of inositol to CDP di cylglyc erol is c t lysed y the enzyme: (A) (B) (C) (D) CTP phosph tid te cytidyl tr ns fer se Phosph tid te phosphohydrol se CDP-di cylglycerol inositol tr nsfer se Ch oline kin se 214. The enzyme glycerol kin se is low idney (D) Adipose tissue
ctivity in (A) Liver (C) Intestine (B) K
222. Synthesis of sphingosine requires the cof ctor (A) NAD (C) NADPH+ (B) NADP (D) ATP 215. The common precursor in the iosynthesis of tri cylglycerol nd phospholipi ds is (A) (B) (C) (D) 1, 2-Di cylglycerol phosph te 1-Acylglycerol 3-phosph te G lycerol 3-phosph te Dihydroxy cetone phosph te 223. Cer mide is formed y the com in tion of sphingosine M lonyl-CoA (B) Acyl-CoA (D) Propionyl-CoA
nd (A) Acetyl-CoA (C)
224. The mino lcohol sphingosine is synthesized in (A) (B) (C) (D) Mitochondri Cytosol Nucleus Endopl smic reticulum 216. Synthesis of polyuns tur ted f tty cids involves the enzyme systems: (A) ( B) (C) (D) Acyl tr nsfer se nd hydr t se Des tur se nd elong se Keto cyl-CoA r educt se nd hydr t se Dihydroxy cetone phosph te 225. The output of free f tty cids from dipose tissue is reduced y (A) Insuli n (B) Gluc gon (C) Growth hormone (D) Epinephrine 226. The princip l ction of i nsulin in dipose tissue is to inhi it the ctivity of the (A) (B) (C) (D) Hormo ne sensitive lipoprotein lip se Glycerol phosph te cyltr nsfer se Acetyl-CoA c r oxyl se Pyruv te dehydrogen se 217. The des tur tion nd ch in elong tion system of polyuns tur ted f tty re enh nced y (A) Insulin (C) Epinephrine (B) Gluc gon (D) Thyroxine
cid
218. Higher r te of lipogenesis is ssoci ted with (A) (B) (C) (D) High proporti on of c r ohydr te in diet Restricted c loric int ke High f t diet Deficiency of insulin 227. In non shivering thermogenesis (A) Glucose is oxidized to l ct te (B) F tty cids uncouple oxid tive phosphoryl tion (C) Eth nol is formed (D) ATP is urne d for he t production 228. Brown dipose tissue is (A) A prominent tissue in hum n (B) Ch r cterised y high content of mitochondri (C) Associ ted with high c tivity of ATP synth se (D) Ch r cterised y low content of cytochromes 219. Ex mple of enzyme specificity: (A) Stereo specificity (B) Re ction specific ity (C) Su str te specificity(D) All of these 220. Phospholip se C tt cks the e ster ond li er ting 1, 2-di cylglycerol nd phosphoryl se t position (A) 1 (C) Both (A) nd (B) (B) 2 (D) 3
154 229. F tty liver is c used due to ccumul tion of (A) F tty ids (B) Cholesterol (D) Tri cylglycerol
cids (C) Phospholip
MCQs IN BIOCHEMISTRY 238. In the iosynthesis of cholesterol, the step which controls the r te nd lo cus of met olic regul tion is (A) Ger nyl pyrophosph te f rnesyl pyrophosph te (B) Squ lene → l nosterol (C) HMG CoA → mev lon te (D) L nosterol → 1, 4-desmethyl l n osterol 239. The cyclis tion of squ lene in m mm ls results in the direct form t ion of the sterol. (A) Cholesterol (C) Sistosterol (B) L nosterol (D) Zymosterol 230. A lipotropic f ctor is (A) Choline (C) C lcium (B) P lmitic n C 231. F tty liver is
lso c used
cid (D) Vit mi
y (A) CH3Cl (C) N 2SO4 (B) CCl4 (D) Ri ofl vin
232. All the enzymes involved in the synthesis of cholesterol re found in (A) ( B) (C) (D) Mitochondri Golgi pp r tus Nucleus Endopl smic reticulum nd cytoso l 240. In the iosynthesis of cholesterol, the r te limiting enzyme is (A) (B) (C) (D) Mev lon te kin se HMG-CoA synthet se HMG-CoA reduct se Cis-prenyl tr nsfer se 233. The source of ll the c r on toms in cholesterol is (A) Acetyl-CoA (C) Pro pionyl-CoA (B) Bic r on te (D) Succinyl-CoA 241. Cholesterol y feed ck mech nism inhi its the ctivity of (A) (B) (C) ( D) HMG-CoA synthet se HMG-CoA reduct se Thil se Mev lon te kin se 234. Two molecules of cetyl-CoA condense to form ceto cetyl-CoA c t lysed A) Thiol se (C) Reduct se (B) Kin se (D) Isomer se
y (
242. The ctivity of HMG-CoA reduct se is inhi ited y (A) (B) (C) (D) A fung l inhi itor mev st tin Pro ucol Nicotinic cid Clofi r te 235. Aceto cetyl-CoA condenses with one more molecule of cetyl-CoA to form (A) (B) (C) (D) Mev lon te Aceto cet te -Hydroxy utyr te 3-Hydroxy 3-methyl-glut rylCoA 243. Hypolipidemic drugs reduce serum cholesterol nd tri cylglycerol. The effec t of clofi r te is ttri uted to (A) Block in sorption from G.I.T. (B) Decre s e in secretion of tri cylglycerol nd cholesterol cont ining VLDL y liver (C) B lock in the re sorption of ile cids (D) Decre sed synthesis of cholesterol 24 4. In iosynthesis of cholesterol trip r nol inhi its the ctivity of the enzyme : (A) (B) (C) (D) ∆24 Reduct se Oxidosqu lene-l nosterol cycl se Isomer se Squ len e epoxid se 236. HMG-CoA is converted to mev lon te y reduction c t lysed y (A) (B) (C) (D ) HMG-CoA synthet se HMG-CoA reduct se Mev lon te kin se Thiol se 237. For reduction enzyme HMG-CoA reduct se requires cof ctor: (A) NADPH (C) NAD (B) NADP (D) FAD
ENZYMES 245. HMG-CoA reduct se ctivity is incre sed y dministr tion of the hormone: ( A) Insulin (C) Epinephrine (B) Gluc gon (D) Glucocorticoids 155 253. Defective enzyme in Hunter’s syndrome is (A) α-L-iduronid se (B) Iduron te sulp h t se (C) Arylsulph t se B (D) C- cetyl tr nsfer se 254. In Hunter’s syndrome (A) (B) (C) (D) There is progressive corne l op city Ker t n sulph te is excreted i n the urine Enzyme defective is rylsulph t se B He ring loss is perceptive 246. The princip l sterol excreted in feces is (A) Coprost nol (C) L nosterol (B ) Zymosterol (D) Desmosterol 247. The princip l r te limiting step in the iosynthesis of ile cids is t th e (A) (B) (C) (D) 7-Hydroxyl se re ction 12 α-Hydroxyl se re ction Conjug tion re ction Deconjug tion re ction 255. An import nt fe ture of Von-Gierke’s dise se is (A) Muscle cr mps (C) Hypogly cemi (B) C rdi c f ilure (D) Respir tory lk losis 248. Hypercholesterolemi is found in (A) (B) (C) (D) X nthom tosis Thyrotoxicos is Hemolytic j undice M l sorption syndrom 256. The ffected org n in Mc Ardle’s syndrome is (A) Liver (C) Liver ) Kidney (D) Skelet l muscle
nd He rt (B
257. Refsum’s dise se is due to deficiency of the enzyme: (A) (B) (C) (D) Pyt nt t e-α-oxid se Glucocere rosid se G l ctocere rosid se Cer mide trihexosid se 249. Hypocholesterolemi is found in (A) (B) (C) (D) Thyrotoxicosis Di litus O structive j undice Nephrotic syndrome
etes mel
250. The m jor source of extr cellul r cholesterol for hum n tissue is (A) (B) ( C) (D) Very low density lipoprotein High density lipoprotein Low density lipopro tein Al umin 258. An import nt finding in Refsum’s dise se is (A) Accumul tion of cer mide trih exoside in the kidney (B) Accumul tion of phyt nic cid in the lood nd tissues (C) Accumul tion of g ngliosides in r in nd spleen (D) Skin eruptions 259. α-G l ctosid se enzyme is defective in (A) (B) (C) (D) T y-s ch’s dise se Refsum’s dise se S ndhoff’s dise se F ry’s dise se 251. Correct ordering of lipoprotein molecules from lowest to the gre ter densit y is (A) (B) (C) (D) LDL, IDL, VLDL, chylomicron Chylomicron, VLDL, IDL, LDL VLD L, IDL, LDL, chylomicron LDL, VLDL, IDL, chylomicron 252. In Hurler’s syndrome, urine shows the presence of (A) (B) (C) (D) Ker t n sul ph te I Chondroitin sulph te Derm t n sulph te nd hep r n sulph te Ker t n sulp h te II 260. The hypothesis to expl in enzyme– su str te complex form tion: (A) (B) (C) (D ) Lock nd key model Induced fit theory Proenzyme theory Both (A) nd (B)
156 261. An import nt finding in T y-s ch’s dise se is (A) Ren l f ilure (B) Accumul t ion of g ngliosides in r in nd spleen (C) C rdi c f ilure (D) Anemi 262. The enzyme deficient in Kr e’s dise se is (A) Hexos minid se A (B) Arylsuph t se A ( C) β-G l ctosid se (D) α-Fucosid se 263. The enzyme cer mid se is deficient in (A) F r er’s dise se (B) F ry’s dise se (C) S ndhoff’s dise se(D) Refsum’s dise se 264. Niem nn-Pick dise se is due to deficiency of the enzyme (A) (B) (C) (D) (A) (B) (C) (D) Cer mid se Glucocere rosid se G l ctocere rosid se Sphingomyelin se Choleste ryl ester hydrol se Hexos minid se A α-Fucosid se Arylsulph t se A MCQs IN BIOCHEMISTRY 269. B ssen-Kornzweig syndrome is due to (A) (B) (C) (D) A sence of Apo-C-II Def ect in Apo-B synthesis A sence of Apo-E A sence of Apo-D 270. Enzyme deficient in Hyper mmonemi thet se Glut min se Ornithine tr nsc r
type II is (A) (B) (C) (D) Glut mine syn moyl se C r moylphosph te synthet se
271. An import nt finding in Hyper mmonemi type II is (A) (B) (C) (D) Incre sed serum glu tmine level Enl rged liver Ment l ret rd tion Incre sed c r moyl pho sph te synthet se level 265. Wolm n’s dise se is due to deficiency of 272. A sence of the enzyme rgininosuccin te synthet se c uses (A) (B) (C) (D) A rgininosuccinic ciduri Hyper rgininemi Tricorrhexis nodos Citrullinemi 266. The enzyme deficient in S ndhoff’s dise se is (A) (B) (C) (D) α-Fucosid se Hexo s minid se A nd B β-G l ctosid se -Glucosid se 273. Tricorrhexis nodos is ch r cteristic finding of (A) (B) (C) (D) Arginino succinic ciduri Citrullinemi Phenylketonuri Hyper rgininemi 267. J m ic n vomiting sickness is due to in ctiv tion of the enzyme (A) (B) (C) (D) Pyruv te c r oxyl se Acyl-Co-A synthet se Acyl-Co-A dehydrogense Thiol se 274. Elev ted lood rgininosuccin te level is found in (A) (B) (C) (D) Hyper rg ininemi Argininosuccinic ciduri Citrullinemi Tyrosinosis 268. Zellweger’s syndrome is due to inherited sence of (A) (B) (C) (D) Peroxisom es Phospholip se A1 Acyl-Co-A dehydrogen se Thiol se 275. Hyper rgininemi , defect in ure synthesis develops due to deficiency of the enzyme: (A) (B) (C) (D) Ornithine tr nsc r moyl se Argininosuccin se Argin se Argininosuccin te synthet se
ENZYMES 276. Al inism is due to deficiency of the enzyme: (A) (B) (C) (D) Phenyl l nine hydroxyl se Tyrosin se p-Hydroxyphenylpyruvic cid oxid se Tyrosine dehydrogen s e 157 284. A coenzyme required in tr ns min tion re ctions is (A) Coenzyme A (C) Bioti n (B) Coenzyme Q (D) Pyridox l phosph te 285. Coenzyme A cont ins vit min which is (A) Thi min (B) Ascor ic cid (C) P ntothenic cid (D) Ni cin mide 286. Co mides cont in vit min which is (A) Fol ic cid (B) Ascor ic cid (C) P ntothenic cid (D) Vit min B12 287. A coenzyme r equired in c r oxyl tion re ctions is (A) Lipoic cid (C) Biotin (B) Coenzyme A (D) All of these 277. Neon t l tyrosinemi is due to deficiency of the enzyme: (A) (B) (C) (D) pHydroxyphenylpyruv te hydroxyl se Fum ryl ceto cet te hydrol se Phenyl l nine hy droxyl se Tyrosine dehydrogen se 278. Which of the following is su str tespecific enzyme? (A) Hexokin se (C) L ct se (B) Thiokin se (D) Aminopeptid se 279. Coenzymes com ine with (A) Proenzymes (C) Holoenzymes (B) Apoenzymes (D) An tienzymes 288. Which of the following coenzyme t kes p rt in tissue respir tion? (A) Coenz yme Q (C) NADP (B) Coenzyme A (D) Co mide 280. Coenzymes re required in which of the following re ctions? (A) (B) (C) (D) Oxid tion-reduction Tr ns min tion Phosphoryl tion All of these 289. The enzyme hexokin se is (D) Lig se
(A) Hydrol se (C) Tr nsfer se (B) Oxidoreduct se
290. Which of the following is (B) Trypsin (D) All of these
proteolytic enzyme? (A) Pepsin (C) Chymotrypsin
281. Which of the following coenzyme t kes p rt in hydrogen tr nsfer re ctions? (A) Tetr hydrofol te (C) Coenzyme Q (B) Coenzyme A (D) Biotin 291. Enzymes which c t lyse inding of two su str tes y cov lent n s (A) Ly ses (C) Lig ses (B) Hydrol ses (D) Oxidoreduct ses
onds
re know
282. Which of the following coenzyme t kes p rt in oxid tion-reduction re ctions ? (A) (B) (C) (D) Pyridox l phosph te Lipoic cid Thi min diphosph te None of th ese 292. The induced fit model of enzyme hell (B) Koshl nd (D) M rkert
ction w s proposed
y (A) Fischer (C) Mitc
283. In conversion of glucose to glucose-6phsoph te, the coenzyme is (A) (B) (C) (D) Mg ++ ATP Both (A) nd (B) None of these 293. Allosteric inhi ition is lso known s (A) (B) (C) (D) Competitive inhi iti on Non-competitive inhi ition Feed ck inhi ition None of these
158 294. An llosteric enzyme is gener lly inhi ited y (A) Initi l su str te of the p thw y (B) Su str te n logues (C) Product of the re ction c t lysed y llost eric enzyme (D) Product of the p thw y 295. When the velocity of n enzym tic re ction equ ls Vm x, su str te concentr tion is (A) H lf of Km (C) Twice the Km ( B) Equ l to Km (D) F r ove the Km MCQs IN BIOCHEMISTRY 302. Serum l ct te dehydrogen se rises in (A) Vir l hep titis (B) Myoc rdi l inf rction (C) C rcinom tosis (D) All of these 303. Which of the following serum en zyme rises in myoc rdi l inf rction: (A) Cre tine kin se (C) LDH (B) GOT (D) All of these 304. From the following myoc rdi l inf rction, the e rliest serum enzyme to rise is (A) Cre tine Kin se (C) GPT 305. Proenzymes: (A) Chymotrysinogen (B) Pepsino gen (C) Both (A) nd (B) (D) None of these 306. Alk line phosph t se is present in (A) Liver (C) Pl cent (B) Bones (D) All of these (B) GOT (D) LDH 296. In Linewe ver-Burk plot, the y-intercept represents (A) Vm x (C) Km (A) (B) (C) (D) (B) Km (D) 1/Km 297. In competitive inhi ition, the inhi itor Competes with the enzyme Irreversi ly inds with the enzyme Binds with the su str te Competes with the su str te 298 Competitive inhi itors (A) Decre se the Km (B) Decre se the Vm x (C) Incre s e the Km (D) Incre se the Vm x 299. Competitive inhi ition c n e relieved y r ising the (A) Enzyme concentr tion (B) Su str te concentr tion (C) Inhi itor con centr tion (D) None of these 300. Physostigmine is competitive inhi itor of (A ) X nthine oxid se (B) Cholinester se (C) C r onic nhydr se (D) Mono mine oxid se 301. C r onic nhydr se is competitively inhi ited y (A) Allopurinol (C) Ami nopterin (B) Acet zol mide (D) Neostigmine 307. Which of the following isoenzyme of l ct te dehydrogen se is r ised in seru m in myoc rdi l inf rction: (A) LD1 (C) LD1 nd LD2 (B) LD2 (D) LD5 308. Enzymes which re lw ys present in n org nism re known s (A) (B) (C) (D ) Induci le enzymes Constitutive enzymes Function l enzymes Apoenzymes 309. In ctive precursors of enzymes B) Coenzymes (D) Holoenzymes
re known
s (A) Apoenzymes (C) Proenzymes (
310. Whcih of the following is proenzyme? (A) (B) (C) (D) C r oxypeptid se Ami nopeptid se Chymotrypsin Pepsinogen
ENZYMES 311. Allosteric enzymes regul te the form tion of products y (A) (B) (C) (D) Fe ed ck inhi ition Non-competitive inhi ition Competitive inhi ition Repression-d erepression 321. Alk line phosph t se is present in (A) Liver (B) Bones (C) Inte stin l mucos (D) All of these 159 322. All of the following re zinc-cont ining enzymes except (A) Acid Phosph t s e (B) Alk line Phosph t se (C) C r onic nhydr se (D) RNA polymer se 323. All of the following re iron-cont ining enzymes except (A) C r onic nhydr se (B) C t l se (C) Peroxid se (D) Cytochrome oxid se 324. Biotin is coenzyme for (A) Py ruv te dehydrogen se (B) Pyruv te c r oxyl se (C) PEP c r oxykin se (D) Glut m t e pyruv te tr nsmin se 325. Enzymes cceler te the r te of re ctions y (A) Incr e sing the equili rium const nt of re ctions (B) Incre sing the energy of ctiv tion (C) Decre sing the energy of ctiv tion (D) Decre sing the free energy ch n ge of the re ction 326. Kinetics of n llosteric enzyme re expl ined y (A) (B ) (C) (D) Mich elis-Menten equ tion Linewe ver-Burk plot Hill plot All of these 312 Regul tion of some enzymes y cov lent modific tion involves ov l of (A) Acet te (C) Phosph te (B) Sulph te (D) Coenzyme 313. Cov lent modific tion of n enzyme gener lly requires tein kin se (B) cAMP (D) All of these 314. An inorg nic ion required for the ctivity of iv tor (C) Coenzyme (B) Cof ctor (D) None of these
ddition or rem
(A) Hormone (C) Pro
n enzyme is known
s (A) Act
315. The first enzyme found to h ve isoenzymes w s (A) (B) (C) (D) Alk line Phos ph t se L ct te dehydrogen se Acid Phosph t se Cre tine kin se 316. L ct te dehydrogen se is loc ted in (A) Lysosomes (C) Cytosol (B) Mitochond ri (D) Microsomes 317. L ct te dehydrogen se is
(A) Monomer (C) Tetr mer (B) Dimer (D) Hex mer
318. Cerulopl smin is sent in (A) Cirrhosis of liver (B) Wilson’s dise se (C) Me nke’s dise se (D) Copper deficiency 319. Cerulopl smin oxidizes (A) Copper (C) Bot h (A) nd (B) (B) Iron (D) None of these 327. Cov lent modific tion of n enzyme usu lly involves phosphoryl tion / depho sphoryl tion of (A) Serine residue (B) Proline residue (C) Hydroxylysine residue (D) Hydroxyproline residue 320. Cre tine kin se is present in cles (B) Myoc rdium (D) Br in
ll of the following except (A) Liver (C) Mus
160 328. Vm x of n enzyme m y e ffected y (A) pH (B) Temper ture (C) Non-competi tive inhi itors (D) All of these 329. In enzyme ss ys, ll the following re ke pt const nt except (A) Su str te concentr tion (B) Enzyme concentr tion (C) pH ( D) Temper ture 330. If the su str te concentr tion is much elow the km of the e nzyme, the velocity of the re ction is (A) Directly proportion l to su str te co ncentr tion (B) Not ffected y enzyme concentr tion (C) Ne rly equ l to Vm x (D ) Inversely proportion l to su str te concentr tion 331. Enzymes requiring NAD s co-su str te c n e ss yed y me suring ch nge in sor nce t (A) 210 nm (C ) 340 nm (B) 290 nm (D) 365 nm MCQs IN BIOCHEMISTRY 335. The highest energy level is present mongst the following in (A) (B) (C) (D ) 1, 3-Biphosphoglycer te Cre tine phosph te C r moyl phosph te Phosphoenol pyr uv te 336. D ily urin ry uro ilinogen excretion in 5–8 mg (D) 13–20 mg
dult men is (A) 0–4 mg (C) 9–12 mg (B)
337. In o structive j undice, f ec l uro ilinogen is (A) A sent (C) Incre sed (B ) Decre sed (D) Norm l 338. Acetyl-CoA c n s (D) All of these
e formed from (A) Pyruv te (C) ketone
odies (B) F tty
cid
339. Pyruv te is converted into cetyl-CoA y (A) (B) (C) (D) Dec r oxyl tion De hydrogen tion Oxid tive dec r oxyl tion Oxid tive de min tion 332. Different isoenzymes of n enzyme h ve the s me (A) Amino cid sequence (B) Mich elis const nt (C) C t lytic ctivity (D) All of these 333. From the pent p eptide, phe- l -leu-lys rg, phenyl l nine residue is split off y (A) Trypsin (B ) Chymotrypsin (C) Aminopeptid se (D) C r oxypeptid se 334. A high-energy phosph te mong the following is (A) Glucose-6-phosph te (B) Glucose-1-phosph te (C) 1 , 3-Biphoglycer te (D) All of these 340. Conversion of pyruv te into cetyl CoA is c t lysed y (A) (B) (C) (D) Pyru v te dehydrogen se Didrolipoyl cetyl tr nsfer se Dihydrolipoyl dehydrogen se Al l the 3 cting in concert 341. Pyruv te dehydrogen se complex is loc ted in (A) (B) (C) (D) Cytosol Lysoso mes Mitochondri Endopl smic reticulum 342. A fl voprotein in pyruv te dehydrogen se complex is (A) (B) (C) (D) Pyruv t e dehydrogen se Didrolipoyl cetyl tr nsfer se Dihydrolipoyl dehydrogen se None of these
ENZYMES 343. Pyruv te dehydrogen se complex is regul ted y (A) (B) (C) (D) Cov lent mod ific tion Allosteric regul tion Both (A) nd (B) None of these 161 352. All of the following re intermedi tes of citric osuccin te (C) Pyruv te (B) Ox lo cet te (D) Fum r te
cid cycle except (A) Ox l
344. An llosteric inhi itor of pyruv te dehydrogen se is (A) Acetyl CoA (C) NAD H 345. Ri ozymes: (A) RNA enzyme (B) Non-protein enzymes (C) C t lyst function ( D) All of these 346. In citric cid cycle, NAD is reduced in (A) One re ctions ( C) Three re ctions (B) Two re ctions (D) Four re ctions (B) ATP (D) Pyruv te 353. All of the following intermedi tes of citric cid cycle c n e formed from mino cids except (A) α-Ketoglut r te (C) M l te (B) Fum r te (D) Ox lo cet te 354. Glycolytic p thw y is loc ted in (A) Mitochondri (C) Microsomes (A) Acetyl CoA (C) Pyruv te (B) Cytosol (D) Nucleus (B) L ct te (D) CO2 nd H2O 355. End product of
ero ic glycolysis is
347. Among citric cid cycle enzymes, fl voprotein is (A) (B) (C) (D) M l te F um r se Succin te dehrogen se Isocitr te dehrogen se 356. During f sting, glucose is phosphoryl ted m inly (A) nd (B) (B) Glucokin se (D) None of these
y (A) Hexokin se (C) Both
357. Glucokin se is found in (A) Muscles (C) Liver (B) Br in (D) All of these 348. In citric cid cycle, GDP is phosphoryl ted y (A) (B) (C) (D) (A) (B) (C) (D) Succin te dehydrogen se Aconit se Succin te thiokin se Fum rse M l te dehydr ogen se α-Ketoglut r te dehydrogen se Succin te dehydrogen se Isocitr te dehydroge n se 358. In n ero ic glycolysis, energy yield from e ch molecule of glucose is (A) 2 ATP equiv lents (B) 8 ATP equiv lents (C) 30 ATP equiv lents(D) 38 ATP equiv l ents 359. Which of the following is n llosteric enzyme? (A) (B) (C) (D) Phosph ohexose isomer se Phosphotriose isomer se L ct te dehydrogen se Phosphofructokin se (B) Br in (D) Erythrocytes 349. M lon te is
n inhi itor of
350. Isocitr te dehydrogen se is llosteric lly inhi ited (C) ATP (B) α-Ketoglut r te (D) NADH 360. Glycolysis is
y (A) Ox losuccin te
n ero ic in (A) Liver (C) Kidneys
351. All of the following re llosteric enzymes except (A) (B) (C) (D) Citr te synthet se -Ketoglut r te dehdrogen se Succin te thiokin se Succin te dehydroge n se 361. Phosphofructokin se is llosteric lly inhi ited -1, 6- iphosph te L ct te Pyruv te Citr te
y (A) (B) (C) (D) Fructose
162 362. Glucose-6-phosph te is n llosteric inhi itor of (A) (B) (C) (D) Glucokin se Hexokin se Phosphohexose isomer se None of these MCQs IN BIOCHEMISTRY 370. Glucose-6-phosph te dehydrogen se is induced y (A) (B) (C) (D) 6-Phosphogl uconol ctone Glucose-6-phosph te Ri ose-5-phosph te Insulin 363. ATP is co-su str te s well s n llosteric inhi itor of (A) (B) (C) (D) Phosphofructokin se Hexokin se Glucokin se None of these 371. The dec r oxyl tion re ction in HMP shunt is c t lysed y (A) (B) (C) (D) G luconol ctone hydrol se 6-Phosphoglucon te dehydrogen se 6-Phosphoglucon te dec r oxyl se Tr ns ldol se 364. Complete oxid tion of one molecule of glucose into CO2 nd H2O yields (A) ( B) (C) (D) 8 ATP equiv lents 15 ATP equiv lents 30 ATP equiv lents 38 ATP equiv lents 372. The first pentose formed in HMP shunt is (A) Ri ose-5-phosph te (B) Ri ulos e-5-phosph te (C) Xylose-5-phosph te (D) Xylulose-5-phosph te 373. The regul tor y enzyme in HMP shunt is (A) (B) (C) (D) Glucose-6-phosph te dehydrogen se 6-Pho sphoglucon te dehydrogen se Both (A) nd (B) None of these 365. A unique y-product of glycolysis in erythrocytes is (A) (B) (C) (D) L ct t e 1, 3-Biphosphoglycer te 2, 3-Biphosphoglycer te All of these 374. The r te of HMP shunt re ctions is (A) (B) (C) (D) Incre sed y Insulin Inc re sed in di etes mellitus Incre sed y gluc gons Incre sed in st rv tion 366. Which of the following enzymes incorpor tes inorg nic phosph te into the su str te? (A) (B) (C) (D) Phosphoglycer te kin se Glycer ldehyde-3-phosph te dehy drogen se Pyruv te kin se Enol se 375. Glycogenesis requires (A) GTP (C) UTP (B) CTP (D) None of these 367. R poport-Lue ering cycle is loc ted in (A) Liver (C) Br in (B) Muscles (D) Erythrocytes 376. Glycogen synthet se c t lyses the form tion of (A) (B) (C) (D) α−1, 4 Glycosidi c bonds α−1, 6 Glycosidic bonds Both (A) and (B) None of these 368. Glycerol can enter lycolytic pathway via (A) (B) (C) (D) Dihydroxyacetone phosphate 1, 3 Biphospo lycerate 3 Phospho lycerate 2 Phospho lycerate 377. Glyco enoloysis is increased by (A) Gluca on (C) Epinephrine (B) Insulin (D ) cAMP 369. HMP shunt is present in (A) Erythrocytes (C) Testes (B) Liver (D) All of th ese 378. Hepatic lyco enoloysis is increased by (A) Insulin (C) Epinephrine (B) Glu ca on (D) Glucocorticoids
ENZYMES 379. Glyco en phosphorylase liberates the followin from lyco en (A) (B) (C) (D ) Glucose Glucose 6 phosphate Glucose 1 phosphate Maltose 388. Gluconeo enesis d oes not occur in (A) Brain (C) Muscles (A) Glycerol (C) Alanine (B) Kidneys (D) Liver (B) Lactate (D) Leucine 163 389. Glucose cannot be synthesized from 380. After the action of phosphorylase, in (C) Amylose (B) dextrin (D) Maltose
lyco en is converted into (A) Amylopect
390. Coenzyme for phosphoenolpyruvate carboxykinase is (A) ATP (C) GTP (A) Strep tokinase (C) Riboflavinase (B) ADP (D) GDP (B) Aspara inase (D) Both (A) and (B) 381. Glucose 1 phosphate liberated from lyco en cannot be converted into free lucose in (A) Liver (C) Muscles (A) (B) (C) (D) (B) Kidneys (D) Brain 391. Therapeutic enzymes: 382. A coenzyme present in phosphorylase is NAD Pyridoxal phosphate Thiamin pyro phosphate Coenzyme A 392. A luconeo enic enzyme amon the followin is (A) (B) (C) (D) Phosphofructo kinase Pyruvate kinase Phosphoenol pyruvate carboxykinase Glucokinase 383. If lucose 1 phosphate formed by lyco enoloysis in muscles is oxidized to CO2 and H2O, the ener y yield will be (A) 2 ATP equivalents (B) 3 ATP equivalent s (C) 4 ATP equivalents (D) 8 ATP equivalents 384. A molecule of phosphorylase k inase is made up of (A) 4 subunits (C) 12 subunits 385. Cyclic AMP binds to (A) (B) (C) (D) Catalytic subunits of protein kinase Re ulatory subunits of protein kinase Catalytic subunits of phosphorylase kinase Re ulatory subunits of phospho rylase kinase (B) Kidneys (D) Thrombocytes (B) 8 subunits (D) 16 subunits 393. Glucose 6 phosphatase and PEP carboxy kinase are re ulated by (A) (B) (C) ( D) Covalent modification Allosteric re ulation Induction and repression All of t hese 394. The maximum possible chain len th of fatty acids formed in the pathway of d e novo synthesis is (A) 16 Carbon atoms (B) 18 Carbon atoms (C) 20 Carbon atoms (D) 24 Carbon atoms 395. Acetyl CoA required for de novo synthesis of fatty acid s is obtained from (A) (B) (C) (D) Breakdown of existin fatty acids Ketone bodi es Acetate Pyruvate 386. Glucose is the only source of ener y for (A) Myocardium (C) Erythrocytes 387. Glycerol 3 phosphate for the synthesis of tri lycerides in adipose tissue i s derived from (A) Phosphatidic acid (B) Diacyl lycerol (C) Glycerol (D) Glucose 396. Formation of acetyl CoA from pyruvate for de novo synthesis of fatty acids requires (A) (B) (C) (D) Pyruvate dehydro enase complex Citrate synthetase ATP c itrate lyase All of these
164 397. The major site for elon ation of medium chain fatty acids is (A) Mitochondr ia (C) Microsomes (A) NADPH (C) Malonyl CoA (B) Cytosol (D) All of these (B) Ace tyl CoA (D) None of these MCQs IN BIOCHEMISTRY 405. Which one of the followin cofactors must be utilized durin the conversion of acetyl CoA to malonyl CoA? (A) TPP (C) NAD+ (B) ACP (D) Biotin 398. β-oxidation of fatty acids is inhi ited
y
399. The enzyme regulating extramitochondrial fatty acid synthesis is (A) (B) (C ) (D) Thioesterase Acetyl CoA car oxylase Acyl transferase Multi-enzyme complex (B) Insulin (D) None of these 406. Which one of the follo ing enzymes requires a coenzyme derived from the vit amin hose structure is sho n elo ? (A) (B) (C) (D) Enoyl CoA hydratase Phospho fructokinase Glucose-6-phosphatase Glucose-6-phosphate dehydrogenase 400. Acetyl CoA car oxylase is activated
y (A) Citrate (C) Both (A) and (B)
407. Coenzymes derived from the vitamin sho n elo are required y enzymes invo lved in the synthesis of hich of the follo ing? (A) ATP (C) CTP (B) UTP (D) NAD H 401. All the follo ing statements a out acetyl CoA car oxylase are true except: (A) (B) (C) (D) It is activated y citrate It is inhi ited y palmitoyl CoA It c an undergo covalent modification Its dephosphorylated form is inactive 408. Coenzymes derived from the vitamin sho n elo are required y hich of the follo ing enzymes? (A) (B) (C) (D) (A) (B) (C) (D) Lactate dehydrogenase Glutam ate dehydrogenase Pyruvate dehydrogenase Malate dehydrogenase U iquinone CoA Pyr uvate dehydrogenase Lipoic acid (B) Iron (D) Methylco alamine 402. All the follo ing statements a out acetyl CoA car oxylase are true except ( A) It is required for de novo synthesis of fatty acids (B) It is required for mi tochondrial elongation of fatty acids (C) It is required for microsomal elongati on of fatty acids (D) Insulin converts its inactive form into its active form 40 3. Both Acyl carrier protein (ACP) of fatty acid synthetase and coenzyme (CoA) a re (A) (B) (C) (D) Contain reactive phosphorylated Contain thymidine Contain pho sphopantetheine reactive groups Contain cystine reactive groups 409. All the follo ing are coenzymes except 410. Which of the follo ing is not a cofactor? (A) Mg (C) Cu 411. All the follo ing compounds are mem ers of the electron transport chain exc ept (A) U iquinone (C) NAD (A) (B) (C) (D) (B) Carnitine (D) FAD 404. Which one of the follo ing transfers acyl groups? (A) (B) (C) (D) Thiamine pyrophosphate Lipomide ATP NADH 412. Thiamine is essential for Pyruvate dehydrogenase Isocitrate dehydrogenase S uccinate dehydrogenase Acetyl CoA synthetase
ENZYMES 413. Adenylate cyclase is activated y (A) Insulin (B) Glucagon (C) Prostaglandi n E1 (D) Ca2+ ions 414. Maximum enzyme activity is o served at (A) Acidic pH (C) Basic pH (B) Neutral pH (D) Optimum pH 165 422. A su strate for the enzyme aldolase is (A) (B) (C) (D) (A) (B) (C) (D) gala ctose-6-phosphate isocitric acid Glucose-1-phosphate Fructose 1, 6 diphosphate T hiamine pyrophosphate, FAD, NAD+ Flavin mononucleotide NADP+ NAD+ only (B) Panto thenic acid (D) Thiamine 423. Decar oxylation of α-keto
cids requires
415. Which of the following is known s one forming enzyme? (A) (B) (C) (D) (A) (B) (C) (D) Alk line phosph t se Acid phosph t se Leucine minopeptid se γ lutam yl transpeptidase Intra molecular rearran ement Breakin of hydro en bonds Coval ent modification Polymerisation 424. Coenzyme A contains the vitamin: (A) Riboflavin (C) Pyridoxine 416. Conversion of pepsino en to pepsin is 425. Which of the followin is not a component of coenzyme A? (A) Adenylic acid (B) Pantothenic acid (C) β -mercaptoethylamine (D) Deoxyadenylic acid 426. Malic e nzyme convers malic acid, in the presence of NADP+ to Pyruvic acid. This reactio n is a/an (A) (B) (C) (D) Decar oxylation Decar oxylation and Dehydrogenation De hydrogenation Oxidation 417. Which of the follo ing is not having an apoenzyme and coenzyme? (A) (B) (C) (D) Lactate dehydrogenase Succinate dehydrogenase Malate dehydrogenase Pepsin ( B) Lyase (D) Oxido reductase 418. Pyruvate dehydrogenase is a/an (A) Isomerase (C) Ligase (A) (B) (C) (D) 419. Homogentisic oxidase is an Oxidase Monooxygenase Dioxygenase Anaerotic dehy drogenase 427. The follo ing reaction is characteristic of hat type of enzymes? 2H2O2 → 2H2 O + O2 (A) (B) (C) (D) Peroxides Catalase Dehydrogenase Copper containing oxidas es 420. Isocitrate dehydrogenase can use __________ as a cofactor. (B) NADP+ only ( A) NAD+ only (C) NAD+ or NADP+ (D) FMN and FAD 421. The rate of most enzyme cata lysed reactions changes ith pH. As the pH increases, this rate (A) (B) (C) (D) reaches a minimum, then increases reaches a maximum, then decreases increases de creases 428. Of Which ar urg’s yello enzyme contains as a prosthetic group? (A) (B) (C) (D) Thiamine pyrophosphate Biotin NAD+ Ri oflavin-5-phosphate 429. Dehydrogenases utilize, as coenzymes, all of the follo ing except (A) NAD+ (C) FAD (B) NADP+ (D) FH4
166 430. Urea is produced physiologically y the action of the enzyme: (A) Urease (C ) Arginase 431. Urease is a (A) Lyase (C) Isomerase (B) Ligase (D) Hydrolase (B) Glutaminase (D) None of these MCQs IN BIOCHEMISTRY 438. Immo ilized enzymes: (A) (B) (C) (D) Potentiation of activity Presentation of activity Preparation of activity All of these 439. This catalyzes formation of CoA derivatives from fatty acid, CoA and ATP: ( A) (B) (C) (D) Acyl CoA dehydrogenase Enoyl hydrase β-OH acyl CoA dehydrogenase Th io kinase 432. Velocity maximum for an enzyme at half the su strate concentration gives (A ) (B) (C) (D) The molecular eight of the enzyme Km value Isoelectric pH Pk valu e 440. Fructose 2, 3 i phosphate is a po erful allosteric activator of (A) (B) (C ) (D) Fructose 1, 6 diphosphatase Phosphofructokinase Hexokinase Fructokinase 433. Which of the follo ing amino acid has een sho n as one of the active site of phosphoglucomutase? (A) Lysine (C) Serine (B) Tyrosine (D) Histidine 441. ‘Clearing factor’ is (A) (B) (C) (D) Lipoprotein lipase Crotonase 7-dehydro cho lesterol β-sitosterol (B) β-glucosides (D) Dextrins 434. The inhi ition of succinate dehydrogenase y malonate y (A) (B) (C) (D) Co mpetitive inhi ition Non-competitive inhi ition Uncompetitive inhi ition Feed ac k inhi ition 442. Maltase attacks only (A) α-glucosides (C) St rch 443. Pepsin is (A) Exo-pepti d se (B) Endo-peptid se (C) C r oxy peptid se(D) Amino peptid se 444. An enzyme in s liv which hydrolyzes st rch is (A) Pepsinogen (C) α-Amyl se (B) Chymotrysin (D) M l te 435. Co mide coenzymes min B12
re (A) Vit min B1 (C) Pyridoxine (B) Ri ofl vin (D) Vit
436. The isozyme CK-MB is specific lly incre sed in the lood of p tients who h d (A) (B) (C) (D) Skelet l muscle dise se Recent myoc rdi l inf rction Infective hep titis Myxoedem 437. FAD cont ining enzyme, c t lyzing form tion of α, β uns tur ted f tty cyl CoA deriv tive. (A) (B) (C) (D) Acyl CoA dehydrogen se Enoyl hydr se β-OH cyl CoA deh ydrogen se Thiol se 445. If coenzyme is required in n enzyme re ction, the former usu lly h s the function of (A) Acting s n cceptor for one of the cle v ge products of the s u str te (B) Enh ncing the specificity of the po enzyme (C) Incre sing the num er of receptor sites of the po enzyme (D) Activ ting the su str te
ENZYMES 446. The Mich ehis-Menten hypothesis: (A) Postul tes the form tion of n enzyme su str te complex (B) En les us to c lcul te the isoelectric point of n enzyme (C) St tes th t the r te of chemic l re ction m y e independent of su str te concentr tion (D) St tes th t the re ction r te is proportion l to su str te co ncentr tion 447. Sch rdinger’s enzyme is (A) L ct te dehydrogen se (B) X nthine de hydrogen se (C) Uric oxid se (D) L mino cid dehydrogen se 448. Tryptoph n pyro l se is currently known s (A) Tryptoph n de min se (B) Tryptoph n dioxygen se ( C) Tryptoph n mono oxygen se (D) Tryptoph n dec r oxyl se 449. An enzyme which rings out lysis of cteri l cell w ll is (A) Amyl se (C) Trypsin (B) Lysozyme (D) Lip se 167 (B) Ch nging the specificity of the enzyme for the su str te (C) Ch nging the co nform tion of the enzyme y inding to site other th n c t lytic site (D) Ch n ging the n ture of the products formed 454. Which of the following regul tory re ctions involves reversi le cov lent modific tion of n enzyme? (A) (B) (C) (D ) Phosphoryl tion of serine OH on the enzyme Allosteric modul tion Competitive i nhi ition Non-competitive inhi ition 455. A competitive inhi itor of n enzyme h s which of the following properties? (A) (B) (C) (D) It is frequently feed ck inhi itor It ecomes cov lently tt ched to n enzyme It decre ses the Vm x It interferes with su str te inding to the enzyme 456. When [s] is equ l to Km, which of the following conditions exist? (A) H lf the enzyme molecules re ound to su str te (B) The velocity of the re ction is equ l to Vm x (C) The velocity of the re ction is independent of su str te conce ntr tion (D) Enzyme is completely s tur ted with su str te 457. Which of the fol lowing st tements out n enzyme exhi iting llosteric kinetics with cooper tiv e inter ction is f lse? (A) A plot of V-Vk [s] h s sigm id l sh pe (B) An inhi itor m y incre se the pp rent Km (C) Line we ver Bnrk plot is useful for deter mining Km nd Vm x (D) Remov l of llosteric inhi itor m y result in hyper olic V-S [s] plot 458. P ntothenic cid cts on (A) NADP (C) FAD (B) NADPH (D) CoA 450. Trypsin h s no
ction on (A) Hemoglo in (C) Histone (B) Al umin (D) DNA
451. Multiple forms of the s me enzymes (B) Isoenzymes (D) Pre-enzymes
re known
s (A) Zymogens (C) Proenzymes
452. In non-competitive enzyme ction (A) Vm x is incre sed (B) App rent km is i ncre sed (C) App rent km is decre sed (D) Concentr tion of ctive enzyme molecul e is reduced 453. An llosteric enzyme influences the enzyme ctivity y (A) Com petiting for the c t lytic site with the su str te 459. Vit min deficiency th t c uses f tty liver includes E (C) Retionic cid (B) Pyridoxine (D) P ntothenic cid
ll except (A) Vit min
168 460. In which of the following types of enzymes n inducer is not required? (A) Inhi ited enzyme (B) Cooper tive enzyme (C) Allosteric enzyme (D) Constitutive e nzyme 461. In which of the following types of enzyme w ter m y e dded to C—C d ou le ond without re king the ond? (A) Hydrol se (C) Hydroxyl se (B) Hydr t s e (D) Ester se MCQs IN BIOCHEMISTRY 467. The est test for cute p ncre titis in the presence of mumps is (A) (B) (C ) (D) A serologic l test for mumps Serum myl se Urin ry myl se Serum lip se 468. The slow moving fr ction of LDH is typic lly incre sed in p ncre s with (A) (B) (C) (D) Cere rov scul r ccidents Acute myoc rdi l inf rction Acute p ncre titis Acute vir l hep tits 462. ‘Lock’ nd ‘Key’ model of enzyme ction proposed y Fisher implies th t (A) The ive site is flexi le nd djusts to su str te (B) The ctive site requires remov l of PO4 group (C) The ctive site is complement ry in sh pe to th t of the su str te (D) Su str tes ch nge conform tion prior to ctive site inter ction 463. In competitive inhi ition of enzyme ction (A) (B) (C) (D) The pp rent Km is de cre sed The pp rent Km is incre sed Vm x is decre sed App rent concentr tion of enzyme molecules decre sed 469. Which of the following enzyme typic lly elev ted in lcoholism? (A) (B) (C) (D) Serum ALP Serum GOT Serum γ GT Serum acid phosphatase 470. Patients with hepatocellular jaundice, as compared to those with purely obs tructive jaundice tend to have (A) (B) (C) (D) Lower serum ALP, LDH and AST acti vity Lower serum ALP, Hi her LDH and AST activity Hi her serum ALP, LDH and AST activity Hi her serum ALP, Lower LDH and AST activity 464. In competitive inhibition which of the followin kinetic effect is true ? ( A) (B) (C) (D) Decreases both Km and Vmax Increases both Km and Vmax Decreases K m without affectin Vmax Increases Km without affectin Vmax 471. If results of the serum bilirubin, serum ALP, LDH and AST determinations su est obstructive jaundice, the best confirmatory test would be the estimation o f (A) (B) (C) (D) Serum ALT Serum 5’ nucleotidase Serum Pseudo cholinesterase None of these 465. Enzymes increase the rates of reactions by (A) Increasin the free ener y o f activation (B) Decreasin the ener y of activation (C) Chan in the equilibriu m constant of the reaction (D) Increasin the free ener y chan e of the reaction 466. The most useful test for the dia nosis of acute hemorrha ic pancreatitis d urin the first few days is (A) Urinary lipase test (B) Serum calcium (C) Urinar y amylase (D) Serum amylase 472. Which enzyme estimation will be helpful in differentiatin the elevated ser um ALP found in obstructive jaundice as well as bone disorders? (A) Serum AST (C ) Serum LDH (B) Serum ALT (D) Serum γ GT 473. Cardiac muscle contains which of the followin CK osoenzyme? (A) BB only (B ) MM and BB only (C) MM, BB and MB (D) MM and MB only
ct
ENZYMES 474. Liver and skeletol measle disorders are characterized by on disk proportion ate increase in which of the LDH isoenzyme fraction? (A) LDH 1 (B) LDH 1 and LDH 2 (C) LDH 3 and LDH 4 (D) LDH 2 and LDH 3 (E) LDH 5 475. On the third day follo win onset of acute myocardial infarction, which enzyme estimation will have the best predictive value? (A) Serum AST (C) Serum ALT (B) Serum CK (D) Serum LDH 169 482. Diastase can be used for the hydrolysis can be used for the hydrolysis of ( A) Sucrose (C) Cellulose (B) Starch (D) Maltose 483. Which of the followin statements is true? (A) Enzymes have names endin as e (B) Enzymes are hi hly specific in their action (C) Enzymes are livin or anis ms (D) Enzymes et activated on heatin 484. Enzymes activity is controlled by ( A) pH of the solution (B) Temperature (C) Concentration of the enzyme (D) Concen tration of the substrate (E) All of these 485. Which of the followin is not tru e re ardin enzymes? (A) They catalyze only a particular type of reaction (B) Th ey remain active even after separation from the source (C) They are destroyed af ter the completion of the reaction they catalyse (D) They are irreversibly destr oyed at hi h temperature (E) Their activity depends on the pH of the solution 48 6 The number of enzymes known is about (A) 10,000 (C) 50 (B) 100 (D) 26 476. Serum AST activity is not characteristically elevated as the result of (A) (B) (C) (D) Myocardial infarction Passive con estion of liver Muscular dystrophi es Peptic ulcer 477. On which day followin acute myocardial infarction the estimation of serum AST will be of reatest si nificance? (A) First day (C) Third day (B) Second day (D) Fourth day 478. In which diseases be released in plasma? eart, Kidney and R.B.C sent low concentration of these
of the followin or ans, isoenzymes LDH 1 and LDH 2 will (A) (B) (C) (D) (A) (B) (C) (D) Kidney, R.B.C and Liver H Heart, Kidney and Liver Heart, Lun s and Brain totally ab in plastic important for dia nosis of several disease All
479. Plasma non functional enzymes are 487. Nicotine present in tobacco is a/an (A) Alkaloid (C) Steroid (B) Terpene (D ) Protein 480. Pyruvate dehydro enase contains all except (A) Biotin (C) FAD (B) NAD (D) C oA 488. The poisonous alkaloid present in the oil of hemlock is (A) Cocaine (C) Qui nine (B) Nicotine (D) Morphine 481. An increase in LDH 5 enzyme is seen in the followin except (A) Acute hepat itis (B) Muscular distrophies (C) Breast carcinoma (D) Pulmonary embolism 489. Alkaloids are usually purified by extraction with (A) Ether (C) NaOH (B) Di l HCl (D) Chloroform
170 490. The number of N MC roups in alkaloids is best estimate with the help of (A ) HI (C) (CH3CO)2 CO (A) (B) (C) (D) (A) (B) (C) (D) (B) H2SO4 (D) CH3 M I MCQs IN BIOCHEMISTRY (D) Be localized intracellularly (E) Be a catalyst 497. LDH assays are most usef ul in dia nosin diseases of the (A) Heart (C) Brain (B) Pancreas (D) Kidney 491. A competitive inhibitor of an enzyme Increases Km without affectin Vmax De creases Km without affectin Vmax Increases Vmax without affectin Km Decreases both Vmax and Km Numerically equal to ½ Vmax Dependent on the enzyme concentration Independent of pH Numerically equal to the substrate concentration that ives h alf maximal velocity 498. The chemical forces that bind most coenzymes and substrates to enzymes such as LDH are (A) Hydro en bonds (B) Peptide bonds (C) Coordinate bonds (D) Covale nt bonds 499. How many different proteins may be present in normal LDH? (A) One (C) Three (B) Two (D) Four 492. The Michaelis constant, Km is 493. The rate of an enzyme catalyzed reaction was measured usin several substra te concentrations that were much lower than K m, the dependence of reaction velo city on substrate concentration can best be described as (A) (B) (C) (D) Indepen dent of enzyme concentration A constant fraction of Vmax Equal to Km Proportiona l to the substrate concentration 500. All the isoenzymes function with the coenzyme: (A) NADP+ (C) Lipoate (B) FA D (D) NAD+ 501. ‘Lock’ and ‘Key’ theory was proposed by (A) Sorenson (C) Mehler (B) Fischer (D) San er 494. The presence of a non competitive inhibitor (A) Leads to both an increase i n the Vmax of a reaction and an increase in Km (B) Leads to a decrease in the ob served Vmax (C) Leads to a decrease in Km and Vmax (D) Leads to an increase in K m without affectin Vmax 495. Which one of the followin statements is not chara cteristic of allosteric enzymes? (A) They frequently catalyze a committed step e arly in a metabolic pathway (B) They are often composed of subunits (C) They fol low Michaelis Menten kinetics (D) They frequently show cooperativity for substra te bindin 496. The abnormal isoenzyme need not (A) Be an oxidoreductase (B) Hav e any coenzyme (C) Require ATP 502. Which of the followin B) Lipase (D) Lysine
forms part of a coenzyme? (A) Zn 2+ (C) Vitamin B2 (
503. The shape of an enzyme and consequently its activity can be reversibly alte red from moment to moment by (A) Heat (B) Amino acid substrate (C) Allosteric su bunits (D) Sulfur substitutions 504. Which one of the followin re ulatory actio ns involves a reversible covalent modification of the enzyme? (A) (B) (C) (D) Ph osphorylation of ser OH on the enzyme Allosteric modulation Competitive inhibiti on Non competitive inhibition 505. An enzyme is a (A) Carbohydrate (C) Protein (B) Lipid (D) Nucleic acid
ENZYMES 506. An enzyme promotes a chemical reaction by (A) Lowerin the ener y of activa tion (B) Causin the release of heat which acts as a primer (C) Increasin molec ular motion (D) Chan in the free ener y difference between substrate and produc t 507. In most metabolic pathways, all needed enzymes are arran ed to ether in a multienzyme complex within a (A) (B) (C) (D) Solution of ATP Membrane Quanterna ry protein Coenzyme (C) Carboxyl roup of the amino acids (D) Exposed sulfur bon ds 513. Allosteric enzymes contain (A) Multiple subunits (B) Sin le chain (C) Tw o chains (D) Three chains 171 514. Isoenzymes of lactate dehydro enase are useful for the dia nosis of (A) Hea rt disease (C) Liver disease (B) Kidney disease (D) Both (A) and (C) 515. IUB had divided enzymes into how many classes? (A) 6 (C) 8 (B) 5 (D) 4 508. An enzyme catalyzes the conversion of an aldose su ar to a ketose su ar wou ld be classified as one of the (A) Transferases (B) Isomerases (C) Oxido reducta ses (D) Hydrolases 509. The function of an enzyme is to (A) Cause chemical react ions that would not otherwise take place (B) Chan e the rates of chemical reacti ons (C) Control the equilibrium points of reactions (D) Chan e the directions of reactions 510. In which of the followin types of enzymes, water may be added t o a C —C double bond without breakin the bond? (A) Hydrolase (C) Hydroxylase (B) Hydratase (D) Oxy enase 516. The first enzyme isolated, purified and crystallied from Jack bean (Canaval ia) by summer in 1926 was (A) Urease (C) Ribonuclease (B) Insulin (D) Zymase 517. Who su ested that enzymes are proteinaceous? (A) Buchner (C) Sumner (B) Ku hne (D) Pasteur 518. Feedback inhibition of enzyme action is affected by (A) Enzyme (C) End prod ucts (B) Substrate (D) None of these 519. The enzyme that converts lucose to lucose 6 phosphate is (A) Phosphatase (C) Phosphorylase (B) Hexokinase (D) Glucose synthetase 511. Enzymes increases the rate of reactions by (A) Increasin the free ener y o f activation (B) Decreasin the ener y of activation (C) Chan in the equilibriu m constant of the reaction (D) Increasin the free ener y chan e of the reaction 512. The active site of an enzyme is formed by a few of the enzymes: (A) R rou ps of the amino acids (B) Amino roups of the amino acids 520. Enzymes are required in traces because they (A) Have hi h turnover number ( B) Remain unused at the end of reaction and are re used (C) Show cascade effect (D) All correct 521. An or anic substance bound to an enzyme and essential for t he activity of enzyme is called (A) Holoenzyme (C) Coenzyme (B) Apoenzyme (D) Is oenzyme
172 522. Enzyme catalysed reactions occur in (A) Pico seconds (C) Milli seconds (B) Micro seconds (D) None of these MCQs IN BIOCHEMISTRY (C) Dry seeds have more reserve food (D) Seedlin s are tender 531. Coenzymes FMN and FAD are derived from vitamin (A) C (C) B1 (B) B6 (D) B2 523. An enzyme can accelerate a reaction up to (A) 1010 times (C) 10 100 (B) 101 times (D) 10 times times 524. In plants, enzymes occur in (A) Flowers only (C) All livin cells 525. Zymo en is a (A) Vitamin (C) Modulator (B) Enzyme precursor (D) Hormone (B) Leaves o nly (D) Stora e or ans only 532. Template/lock and key theory of enzyme action is supported by (A) Enzymes s peed up reaction (B) Enzymes occur in livin bein s and speed up certain reactio ns (C) Enzymes determine the direction of reaction (D) Compounds similar to subs trate inhibit enzyme activity 533. Combination of apoenzyme coenzyme produces (A ) Prosthetic roup (B) Holoenzyme (C) Enzyme substrate complex (D) Enzyme produc t complex 534. Enzyme inhibition caused by a substance resemblin substrate mole cule is (A) Competitive inhibition (B) Non competitive inhibition (C) Feedback i nhibition (D) Allosteric inhibition 535. An enzyme brin s about (A) Decrease in reaction time (B) Increase in reaction time (C) Increase in activation ener y (D ) Reduction in activation ener y 536. Feedback inhibition of enzyme is influence d by (A) Enzyme (C) End product 537. Coenzyme is (A) Often a vitamin compound (B ) Always an inor anic (B) External factors (D) Substrate and 526. Cofactor (Prosthetic roup) is a part of holoenzyme, it is (A) Inor anic pa rt loosely attached (B) Accessory non protein substance attached firmly (C) Or a nic part attached loosely (D) None of these 527. A protein havin both structura l and enzymatic traits is (A) Myosin (C) Trypsin (B) Colla en (D) Actin 528. Enzymes are different from catalysts in (A) Bein proteinaceous (B) Not use d up in reaction (C) Functional at hi h temperature (D) Havin hi h rate of diff usion 529. Enzymes, vitamins and hormones are common in (A) Bein proteinaceous (B) Bein synthesized in the body of or anisms (C) Enhancin oxidative metabolis m (D) Re ulatin metabolism 530. Dry seeds endure hi her temperature than ermin atin seeds as (A) Hydration is essential for makin enzymes sensitive to temper ature (B) Dry seeds have a hard coverin (C) Always a protein (D) Often a metal
ENZYMES 538. Genetic en ineerin requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease 539. Which is not true about inor anic catalysts and e nzymes? (A) They are specific (B) Inor anic catalysts require specific not neede d by enzymes (C) They are sensitive to pH (D) They speed up the rate of chemical reaction 540. Key and lock hypothesis of enzyme action was iven by (A) Fischer (C) Buchner (B) Koshland (D) Kuhne 173 545. Vitamin B2 is component of coenzyme: (A) (B) (C) (D) Pyridoxal phosphate TP P NAD FMN/FAD 546. Km value of enzyme is substrate concentration at (A) ½ Vmax (C) ½ Vmax (B) 2 Vm ax (D) 4 Vmax 547. Part of enzyme which combines with nonprotein part to form functional enzym e is (A) Apoenzyme (C) Prosthetic roup (B) Coenzyme (D) None of these 548. Who ot Nobel Prize in 1978 for workin on enzymes? (A) Koshland (C) Nass a nd Nass (B) Arber and Nathans (D) H.G. Khorana 541. An example of feedback inhibition is (A) Allosteric inhibition of hexokinas e by lucose6 phosphate (B) Cyanide action on cytochrome (C) Sulpha dru on foli c acid synthesizer bacteria (D) Reaction between succinic dehydro enase and succ inic acid 542. Feedback term refers to (A) Effect of substrate on rate of enzyma tic reaction (B) Effect of end product on rate reaction (C) Effect of enzyme con centration on rate of reaction (D) Effect of external compound on rate of reacti on 543. Allosteric inhibition (A) (B) (C) (D) Makes active site unifit for subst rate Controls excess formation and end product Both (A) and (B) None of these 549. Site of enzyme synthesis in a cell is (A) Ribosomes (C) Gol i bodies (B) RE R (D) All of these 550. The fruit when kept is open, tastes bitter after 2 hours because of (A) (B) (C) (D) Loss of water from juice Decreased concentration of fructose in juice F ermentation by yeast Contamination by bacterial enzymes 551. Hexokinase (Glucose + ATP → Glucose 6– P + ADP) belon s to the cate ory: (A) Tr ansferases (B) Lysases (C) Oxidoreductases (D) Isomerases 552. Which enzyme is c oncerned with transfer of electrons? (A) Desmolase (C) Dehydro enase (B) Hydrola se (D) Transaminase 544. The ratio of enzyme to substrate molecules can be as low as (A) 1 : 100,000 (C) 1 : 10,000 (B) 1 : 500,000 (D) 1 : 1,000 553. The best example of extracellular enzymes (exoenzyme) is (A) (B) (C) (D) Nu cleases Di estive enzymes Succinic dehydro enase None of these
174 554. Which mineral element controls the activity of Nitrate reductase ? (A) Fe ( C) Zn (B) Mo (D) Ca MCQs IN BIOCHEMISTRY (B) NH2 roup of amino acids (C) CO roup of amino acids (D) Sulphur bonds which are exposed 563. Carbonic anhydrase enzyme has maximum turn over number (36 mil lion). Minimum turn over number for an enzyme: (A) DNA polymerase (B) Lysozyme ( C) Penicillase (D) Lactase dehydro enase 564. In cell, di estive enzymes are fou nd mainly in (A) Vacuoles (C) Ribosomes (B) Lysosomes (D) Lomasomes 555. Name the enzyme that acts both as carboxylase at one time and oxy enase at another time. (A) (B) (C) (D) PEP carboxylase RuBP carboxylase Carbonic anyhdras e None of these 556. A metabolic pathways is a (A) Route taken by chemicals (B) Sequence of enzy me facilitated chemical reactions (C) Route taken by an enzyme from one reaction to another (D) Sequence of ori in of or anic molecules 557. The ener y required to start an enzymatic reaction is called (A) Chemical ener y (B) Metabolic ener y (C) Activation ener y (D) Potential ener y 558. Out of the total enzymes pres ent in a cell, a mitochondrion alone has (A) 4% (C) 95% (B) 70% (D) 50% 565. Substrate concentration at which an enzyme attains half its maximum velocit y is (A) Threshold value (B) Michaelis Menton constant (C) Concentration level ( D) None of these 566. Which enzyme hydrolyses starch? (A) Invertase (C) Sucrase (B) Maltase (D) Diastase 559. Creatine phosphokinase isoenzyme is a marker for (A) (B) (C) (D) Kidney dis ease Liver disease Myocardial infarction None of these 567. Enzymes functional in cell or mitochondria are (A) Endoenzymes (C) Apoenzym es (B) Exoenzymes (D) Holoenzymes 560. Which inactivates an enzyme by occupyin its active site? (A) (B) (C) (D) C ompetitive inhibitor Allosteric inhibitor Non competitive inhibitor All of these 568. The enzymes present in the membrane of mitochondria are (A) Flavoproteins a nd cytochromes (B) Fumarase and lipase (C) Enolase and catalase (D) Hexokinase a nd zymase 569. A mitochondrial marker enzyme is (A) Aldolase (B) Amylase (C) Suc cinic dehydro enase (D) Pyruvate dehydro enase 561. Which one is coenzyme? (A) ATP (C) CoQ and CoA (B) Vitamin B and C (D) All of these 562. The active site of an enzyme is formed by (A) R
roup of amino acids
ENZYMES 570. The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (C ) Ribonuclease (B) RNA polymerase (D) Endonuclease 175 579. Transaminase activity needs the Coenzyme: (A) ATP (C) FADT (B) B6 PO4 (D) N AD+ 571. Which of the followin is a microsomal enzyme inducer? (A) Indomethacin (C) Tolbutamide (B) Clofibrate (D) Glutethamide 580. The biosynthesis of urea occurs mainly in the liver: (A) Cytosol (C) Micros omes (B) Mitochondria (D) Nuclei 572. Identify the correct molecule which controls the biosynthesis of proteins i n livin or anisms. (A) DNA (C) Purines (B) RNA (D) Pyrimidines 581. Bile salts make emulsification with fat for the action of (A) Amylose (C) P epsin (B) Lipase (D) Trypsin 573. The tear secretion contains an antibacterial enzyme known as (A) Zymase (C) Lysozyme (B) Diastase (D) Lipase 582. All of the followin compounds are intermediates of TCA cycle except (A) Ma leate (C) Oxaloacetate (B) Pyruvate (D) Fumarate 574. Identify one of the canbonic anhydrase inhibitor that inhibit only luminal carbonic anhydrase enzyme. (A) Methazolamide (B) Acetazolamide (C) Dichlorphenam ide (D) Benzolamide 575. Group transferrin Co enzyme is (A) CoA (C) NADP+ (B) N AD+ (D) FAD+ 583. In conversion of lactic acid to lucose, three reactions of lycolytic path way are circumvented, which of the followin enzymes do not participate? (A) Pyr uvate carboxylase (B) Phosphoenol pyruvate carboxy kinase (C) Pyruvate kinase (D ) Glucose 6 phosphatase 584. In the normal restin state of human most of the bl ood lucose burnt as fuel is consumed by (A) Liver (C) Adipose tissue (B) Brain (D) Muscles 576. The co enzyme containin an automatic hetero rin in the structure is (A) B iotin (B) TPP (C) Su ar Phosphate (D) Co enzyme 577. The example of hydro en tra nsferrin Co enzyme is: (A) B6 PO4 (C) TPP (B) NADP+ (D) ATP 585. A re ulator of the enzyme 6 PO4 (B) Pyruvate (D) GTP
luco en synthase is (A) Citric Acid (C) Glucose
578. Enzyme catalyzed hydrolysis of proteins produces amino acid of the form (A) D (C) L (B) DL (D) Racemic 586. A specific inhibitor for succinate dehydro enase is (A) Arsenite (C) Citrat e (B) Malonate (D) Fluoride
176 ANSWERS 1. A 7. C 13. A 19. B 25. D 31. C 37. A 43. A 49. C 55. C 61. A 67. A 73 . A 79. A 85. B 91. A 97. A 103. A 109. D 115. B 121. A 127. B 133. D 139. B 145 . B 151. B 157. A 163. B 169. A 175. B 181. D 187. D 193. A 199. B 205. B 211. A 217. A 223. B 229. D 235. D 241. B 247. A MCQs IN BIOCHEMISTRY 2. B 8. A 14. B 20. D 26. B 32. A 38. B 44. B 50. B 56. D 62. C 68. C 74. B 80. A 86. B 92. A 98. A 104. C 110. C 116. A 122. C 128. B 134. A 140. C 146. A 152. B 158. A 164. A 170. D 176. A 182. B 188. C 194. C 200. A 206. A 212. A 218. A 224. D 230. A 236. B 242. A 248. A 3. A 9. B 15. D 21. A 27. A 33. B 39. D 45. C 51. B 57. C 63. A 69. B 75. A 81. C 87. D 93. A 99. A 105. A 111. B 117. B 123. C 129. B 135. B 141. D 147. A 153. D 159. A 165. A 171. D 177. C 183. D 189. A 195. A 201. D 207. D 213. D 219. D 225. A 231. B 237. A 243. B 249. A 4. D 10. D 16. A 22. A 28. A 34. C 40. C 46. A 52. A 58. C 64. D 70. B 76. B 82. C 88. D 94. B 100. A 106. D 112. B 118. C 124. D 130. B 136. B 142. B 148. C 15 4. A 160. A 166. B 172. A 178.A 184. C 190. A 196. A 202. C 208. A 214. D 220. D 226. A 232. D 238. C 244. A 250. C 5. C 11. C 17. B 23. B 29. A 35. A 41. D 47. D 53. A 59. A 65. A 71. A 77. C 83. B 89. D 95. A 101. A 107. B 113. D 119. B 125. A 131. C 137. A 143. A 149. A 15 5. A 161. A 167. B 173. C 179. D 185. C 191. C 197. B 203. D 209. A 215. A 221. C 227. B 233. A 239. B 245. A 251. B 6. D 12. D 18. C 24. B 30. B 36. B 42. A 48. B 54. C 60. B 66. D 72. B 78. C 84. D 90. A 96. B 102. B 108. A 114. A 120. C 126. A 132. A 138. B 144. B 150. A 15 6. D 162. A 168. C 174. B 180. B 186. A 192. C 198. B 204. D 210. D 216. A 222. C 228. B 234. A 240. C 246. A 252. C
ENZYMES 253. B 259. D 265. A 271. C 277. B 283. B 289. C 295. D 301. B 307. C 313. D 319 . B 325. C 331. C 337. A 343. C 349. C 355. C 361. D 367. D 373. C 379. C 385. B 391. D 397. C 403. C 409. C 415. A 421. B 427. B 433. C 439. D 445. A 451. B 45 7. D 463. B 469. C 475. D 481. D 487. A 493. C 499. D 505. C 254. D 260. C 266. B 272. A 278. C 284. D 290. D 296. B 302. D 308. B 314. B 320. A 326. C 332. C 3 38. D 344. A 350. C 356. A 362. B 368. A 374. A 380. B 386. C 392. C 398. C 404. B 410. D 416. C 422. D 428. D 434. A 440. B 446. A 452. C 458. B 464. D 470. B 476. D 482. B 488. A 494. B 500. D 506. A 255. C 261. B 267. C 273. D 279. B 285 . C 291. C 297. D 303. D 309. C 315. B 321. D 327. A 333. C 339. C 345. D 351. C 357. C 363. A 369. D 375. C 381. C 387. D 393. C 399. B 405. D 411. B 417. D 42 3. A 429. D 435. D 441. A 447. B 453. C 459. C 465. B 471. B 477. B 483. B 489. B 495. C 501. D 507. B 256. D 262. C 268. A 274. A 280. D 286. D 292. B 298. C 3 04. A 310. D 316. C 322. A 328. D 334. C 340. D 346. C 352. C 358. A 364. D 370. D 376. A 382. B 388. C 394. A 400. C 406. D 412. B 418. D 424. B 430. C 436. B 442.A 448. B 454. A 460. D 466. D 472. D 478. B 484. C 490. A 496. A 502. C 508. B 257. A 263. A 269. B 275. B 281. C 287. C 293. C 299. B 305. B 311. A 317. C 323. A 329. B 335. D 341. C 347. C 353. C 359. D 365. C 371. C 377. B 383. B 389 . D 395. D 401. D 407. A 413. B 419. C 425. D 431. D 437. A 443. B 449. B 455. D 461. B 467. D 473. D 479. D 485. C 491. A 497. A 503. C 509. B 258. B 264. D 27 0. C 276. C 282. B 288. A 294. D 300. B 306. D 312. C 318. B 324. B 330. A 336. A 342. C 348. C 354. B 360. D 366. A 372. B 378. B 384. D 390. C 396. D 402. B 4 08. C 414. D 420. C 426. B 432. B 438. B 444. C 450. D 456. A 462. C 468. D 474. C 480. A 486. C 492. D 498. D 504. A 510. A 177
178 511. B 517. C 523. A 529. D 535. D 541. A 547. C 553. C 559. C 565. B 571. D 577 . D 583. B 512. C 518. C 524. C 530. A 536. C 542. B 548. A 554. A 560. A 566. D 572. A 578. C 584. B 513. A 519. B 525. B 531. D 537. A 543. C 549. B 555. B 56 1. D 567. A 573. C 579. B 585. C 514. D 520. D 526. B 532. D 538. D 544. A 550. D 556. B 562. A 568. A 574. B 580. B 586. B MCQs IN BIOCHEMISTRY 515. A 521. C 527. A 533. B 539. B 545. D 551. C 557. C 563. B 569. C 575. A 581 . B 516. A 522. C 528. A 534. A 540. A 546. D 552. A 558. B 564. B 570. D 576. C 582. B
ENZYMES EXPLANATIONS FOR THE ANSWERS 4. D The functional unit of an enzyme is referred t o as a holoenzyme. It is often made up of an apoenzyme (the protein part) and a coenzyme (the non protein part). 47. D Concentration of enzyme, concentration of substrate, temperature, pH, presence of products, activators and inhibitors are some of the important factors that influence enzyme activity. 89. D It is a str ai ht line raphic representation depictin the relation between substrate conce ntration and enzyme velocity. This plot is commonly employed for the calculation of Km values for enzymes. 133. D Active site is the small re ion of an enzyme w here substrate binds. It is flexible in nature and it exists due to the tertiary structure of proteins. Acidic, basic and hydroxyl amino aicds are frequently fo und at the active site. 179. D There are three broad cate ories of enzyme inhibi tion: (a) Reversible inhibition: The inhibitor binds noncovalently with the enzy me and the inhibition is reversible. Competitive, non competitive and uncompetit ive come under this cate ory. (b) Irreversible inhibition: The inhibitor covalen tly binds with the enzyme which is irreversible. (c) Allosteric inhibition: Cert ain enzymes possessin allosteric sites are re ulated by allosteric effectors. 2 19. D Enzymes are hi hly specific in their action compared with chemical catalys ts. Three types of enzyme specificities are well reco nized. (a) Stereospecifici ty: The enzymes act only on one isomer and therefore exhibit stereoisomerism. e. ., L amino acid oxidase on L amino acids; hexokinase on D hexose (Note: isomera ses do not exhibit stereospecificity). (b) Reaction specificity: The same substr ate can under o different types of reactions, each catalysed by a separate enzym e e. ., amino acids under oin transamination, decarboxylation etc. (c) Substrat e specifity: This may be absolute, relative or broad e. ., urease, li ase, hexok inase. 260. D (a) Lock and Key model (Fischer’s Template 179 (b) 305. C 345. D 391. D 438. B 479. D 514. D 559. C theory): The substrate fits to active site of an enzyme just as a key fits into a proper lock. Thus, the active site of the enzyme is ri id and preshaped where only a specific substrate can bind. Induced fit theory (Koshland model): As per this, the substrate induces a conformational chan e in the enzyme resultin in t he formation of substrate bindin (active) site. Some enzymes are synthesized in an inactive form which are referred to as proenzymes (or zymo ens). They under o irreversible modification to produce active enzymes. e. ., proenzymes – chymotr ypsino en and pepsino en are respectively converted to chymotrypsin and pepsin. The RNAs that can function as enzymes are referred to as ribozymes. They are thu s nonprotein enzymes. It is believed that RNAs were functionin as catalysts bef ore the occurance of proteins durin evolution. Streptokinase is used for cleari n blood clots. Aspara inase is employed in the treatment of leukemias. Certain enzymes can be made to bind to insoluble inor anic matrix (e. ., cyano ens bromi de activated sepharose) to preserve their catalytic activity for lon periods. S uch enzymes are referred to as immobilized enzymes. These enzymes are either tot
ally absent or present at a low concentration in plasma compared to their levels found in tissues. Estimation of plasma non functional enzymes is important for the dia nosis and pro nosis of several diseases. Lactate dehydro enase (LDH) as five distinct isoenzymes (LDH1 … LDH5). Each one is an oli omeric protein compose d of 4 subunits (N and/ or H). Isoenzymes of LDH are important for the dia nosis of heart and liver related disorders i.e., serum LDH1 is elevated in myocardial infarction while LDH5 is increased in liver diseases. Creatine kinase (CK) or c reatine phosphokinase (CPK) exists as 3 isoenzymes. Each isoenzyme is a dimmer c omposed of two subunits (M or B or both). Elevation of CPK2 (MB) in serum is an early reliable dia nostic indication of myocardial infarction.
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CHAPTER 7 ETABOLISM MINERAL METABOLISM 1. When ATP forms AMP (A) (B) (C) (D) Inor anic pyrophosphate is produced Inor a nic phosphorous is produced Phsopha en is produced No ener y is produced 7. If ∆G°= –2.3RT log Keq, the free energy for the re ction will
e + B A C
10moles 10moles (A) –4.6 RT (C) +2.3 RT 10moles (B) –2.3 RT (D) +4.6 RT 2. St nd rd free energy (∆G°) of hydrolysis ∆ of ATP to ADP + Pi is (A) –49.3 KJ/mol (C) –30.5 KJ/mol (B) –4.93 KJ/mol (D) –20.9 KJ/mol 8. Redox potenti l (EO volts) of NAD+/NADH is (A) –0.67 (C) –0.12 (B) –0.32 (D) +0.03 ∆ 3. St nd rd free energy (∆G°) of hydrolysis of ADP to AMP + Pi is (A) –43.3 KJ/mol (C) –27.6 KJ/mol (B) –30.5 KJ/mol (D) –15.9 KJ/mol 9. Redox potenti l (EO volts) of u iquinone, ox/red system is (A) +0.03 (C) +0.1 0 (B) +0.08 (D) +0.29 4. St nd rd free energy (∆G°) of hydrolysis ∆ of phosphoenolpyruv te is (A) –61.9 KJ/mol (C) –14.2 KJ/mol (B) –43.1 KJ/mol (D) –9.2 KJ/mol 10. Redox potenti l (EO volts) of cytochrome C, Fe3+/Fe2+ is (A) –0.29 (C) –0.08 (B) –0.27 (D) +0.22 5. St nd rd free energy (∆G°) of hydrolysis ∆ of cre tine phosph te is (A) -–51.4 KJ/mol (C) –30.5 KJ/mol (B) –43.1 KJ/mol (D) –15.9 KJ/mol 11. The prosthetic group of D) P ntothenic cid
ero ic dehydrogen ses is (A) NAD (C) FAD (B) NADP (
6. The oxid tion-reduction system h ving the highest redox potenti l is (A) (B) (C) (D) U iquinone ox/red Fe3+ cytochrome /Fe2+ Fe3+ cytochrome /Fe2+ NAD+/NAD H 12. Alcohol dehydrogen se from liver cont ins (A) Sodium (C) Zinc (B) Copper (D) M gnesium
182 13. A moly denum cont ining oxid se is (A) (B) (C) (D) Cytochrome oxid se X nthi ne oxid se Glucose oxid se L-Amino cid oxid se MCQs IN BIOCHEMISTRY 22. The sequence of the redox c rrier in respir tory ch in is (A) NAD—FMN—Q—cyt —cyt c1—c yt c—cyt 3 O2 → (B) FMN—Q—NAD—cyt —cyt 3—cyt c1— cyt c O2 → (C) NAD—FMN—Q—cyt c NAD—FMN—Q—cyt —cyt 3—cyt c—cyt c1 O2 → 23. The correct sequence of cytochrome c rrie spir tory ch in is (A) Cyt —cyt c—cyt c1—cyt 3 (B) Cyt 3— cyt —cyt c—cyt c1 (C) Cyt t c1—cyt c—cyt 3 (D) Cyt —cyt 3—cyt c1— cyt c 24. Reducing equiv lents from pyruv te enter the mitochondri l respir tory ch in t (A) FMN (C) Coenzyme Q (B) NAD (D) Cyt 14. A copper cont ining oxid se is (A) (B) (C) (D) Cytochrome oxid se Fl vin mon onucleotide Fl vin denine dinucleotide X nthine oxid se 15. The mitochondri l superoxide dismut se cont ins (A) Mg (C) Co++ ++ (B) Mn (D) Zn ++ ++ 16. Cytosolic superoxide dismut se cont ins (A) Cu2+ (B) Mn 2+ (D) Cu2+ nd Fe2+ 17. Cytochrome oxid se cont ins (A) Cu2+ Fe2+ (D) Cu 2+ 18. Ch r cteristic sorption nd β nds (B) β nd (D) α, β
nd Zn2+ (C) Mn2+
nd Zn2+ (C) Cu2+
nds exhi ited nd γ bands
nd Zn2+
nd Mn2+ (B) Cu2+
y ferrocytochrome: (A) α
nd
nd (C) α
25. Reducin equivalents from succinate enter the mitochondrial respiratory chai n at (A) NAD (C) FAD (B) Coenzyme Q (D) Cyt c 19. Monooxy enases are found in (A) Cytosol (C) Mitochondira (B) Nucleus (D) Mic rosomes 26. The respiratory chain complexes actin as proton pump are (A) I, II and III (C) I, III and IV (B) I, II and IV (D) I and II 20. A component of the respiratory chain in mitochondria is (A) (B) (C) (D) Coen zyme Q Coenzyme A Acetyl coenzyme Coenzyme containin thiamin 27. If the reducin equivalents enter from FAD in the respiratory chain, the pho sphate.oxy en ration (P:O) is (A) 2 (C) 3 (B) 1 (D) 4 21. The redox carriers are rouped into respiratory chain complex (A) (B) (C) (D ) In the inner mitochondrial membrane In mitochondiral matrix On the outer mitoc hondrial membrane On the inner surface of outer mitochondrial membrane 28. If the reducin equivalents enter from NAD in the respiratory chain, the phs phate/oxy en (P:O) is (A) 1 (C) 3 (B) 2 (D) 4
MINERAL METABOLISM 29. One of the site of phsosphorylation in mitochondrial respiratory chain is (A ) Between FMN and coenzyme Q (B) Between coenzyme Q and cyt b (C) Between cytoch rome b and cytochrome c1 (D) Between cytochrome c1 and cytochrome c 30. Rotenone inhibits the respiratory chain at (A) FMN → coenzyme Q (B) NAD → FMN (C) Coenzyme Q → cyt b (D) Cyt b → Cyt c1 31. Activity of cytochrome oxidase is inhibited by (A) S ulphite (C) Arsenite (B) Sulphate (D) Cyanide 183 37. The chemical inhibitin oxidative phosphorylation, Adependent on the transpo rt of adenine nucleotides across the inner mitochondrial membrane is (A) Oli omy cin (C) Dinitrophenol (B) Atractyloside (D) Pentachlorophenol 38. Porphyrins are synthesized in (A) Cytosol (B) Mitochondria (C) Cytosol and m itochondria (D) Rou h endoplasmic reticulum 39. Heme is synthesized from (A) Suc cinyl CoA and lycine (B) Active acetate and lycine (C) Active succinate and al anine (D) Active acetate and alanine 40. In the biosynthesis of the iron protopo rphyrin, the product of the condensation between succinyl CoA and lycine is (A) α-Amino β-keto dipic cid (B) δ-Aminolevulinate (C) Hy roxymethylbilane (D) Uroporphy rinogen I 41. Porphyrin synthesis is inhibite in (A) Mercury poisoning (B) Lea poisoning (C) Manganese poisoning (D) Barium poisoning 42. During synthesis of porphyrins, synthesis of δ-amino levulinic aci occurs in (A) Mitochon ria (B) Cyt osol (C) Both in mitochon ria an cytosol (D) Ribosomes 43. In the biosynthesis of heme, con ensation between succinyl CoA an glycine requires (A) NAD+ (C) NAD H + H + 32. Transfer of re ucing equivalents from succinate ehy rogenase to coenzyme Q is specifically inhibite by (A) Carboxin (C) Pierici in A (B) Oligomycin (D) Ro tenone 33. Chemiosmotic theory for oxi ative phosphorylation has been propose by (A) C hance an Williams (B) Pauling an Corey (C) S. Waugh (D) P. Mitchell 34. The nu mber of ATP pro uce in the oxi ation of 1 molecule of NADPH in oxi ative phosph orylation is (A) Zero (C) 3 (B) 2 (D) 4 35. The coupling of oxi ation an phosphorylation in intact mitochon ria: (A) Pu romycin (C) Streptomycin (B) Oligomycin (D) Gentamycin 36. An uncoupler of oxi ative phosphorylation is (A) Carboxin (C) Amobarbital (B ) Atractylosi e (D) Dinitrocresol (B) FAD (D) B6-phosphate
184 44. In mammalian liver the rate controlling enzyme in porphyrin biosynthesis is (A) ALA synthase (B) ALA hy ratase (C) Uroporphyrinogen I synthase (D) Uroporphy rinogen III cosynthase 45. The con ensation of 2 molecules of δ-aminolevulinate e hy ratase contains (A) ALA synthase (B) ALA hy ratase (C) Uroporphyrinogen synth ase I (D) Uroporphyrinogen synthase III 46. The enzyme δ-aminolevulinate ehy rata se contains (A) Zinc (C) Magnesium (B) Manganese (D) Calcium MCQs IN BIOCHEMISTRY 51. The synthesis of heme from protophyrin III is catalyse by the enzyme: (A) A LA synthase (C) Ferrooxi ase 52. Many xenobiotics (A) (B) (C) (D) Increase hepat ic ALA synthase Decrease hepatic ALA sythase Increase hepatic ALA ehy rase Decr ease hepatic ALA ehy rase (B) Ferrore uctase (D) Ferrochelatase 53. Acute intermittent porphyria (paraoxymal porphyria) is cause ue to eficie ncy of (A) (B) (C) (D) Uroporphyrinogen I synthase ALA synthase Coproporphyrinog en oxi ase Uroporphyrinogen ecarboxylase 54. The major symptom of acute intermittent porphyria inclu es (A) (B) (C) (D) A b ominal pain Photosensitivity No neuropsychiatric signs Dermatitis 47. A cofactor require (C) Mg (B) Mn (D) Fe
for the activity of the enzyme ALA
ehy ratase is (A) Cu
48. The number of molecules of porphobilinogen require for the formation of a t etrapyrrole i.e., a porphyrin is (A) 1 (C) 3 (B) 2 (D) 4 55. The characteristic urinary fin ing in acute intermittent porphyria is (A) (B ) (C) (D) Increase quantity of uroporphyrin Increase quantity of coproporphyri n I Increase quantity of coproporphyrin III Massive quantities of porphobilinog en 49. Conversion of the linear tetrapyrrole hy roxymethylbilane to uroporphyrinoge n III (A) Occurs spontaneously (B) Catalyse by uroporphyrinogen I synthase (C) Catalyse by uroporphyrinogen III cosynthase (D) Catalyse by combine action of uroporphyrinogen I synthase an uroporphyrinogen III cosynthase 50. Conversion of uroporphyrinogen III to coprophyrinogen III is catalyse by the enzyme.: (A) Uroporphyrinogen ecarboxylase (B) Coproporphyrinogen oxi ase (C) Protoporphyrin ogen oxi ase (D) Ferrochelatase 56. The enzyme involve in congenial erythropoietic porphyria is (A) (B) (C) (D) Uroporphyrinogen I synthase Uroporphyrinogen III cosynthase Protoporphyrinogen oxi ase Ferrochelatase 57. Main symptoms of congenital erythropoietic porphyria is (A) Yellowish teeth (B) Photosensitivity (C) Ab ominal pain (D) Brownish urine 58. The probable caus e of porphyria cutanea tar a is eficiency of (A) (B) (C) (D) Uroporphyrinogen o xi ase Coproporphyrinogen oxi ase Protoporphyrinogen oxi ase Uroporphyrinogen I synthase
MINERAL METABOLISM 59. The characteristic urinary fin ing in porphyria cutanea tar a is (A) Increas e quantity of porphobilinogen (B) Increase quantity of re cell protoporphyrin (C) Increase quantity of uroporphyrin (D) Increase quantity of δ-ALA 60. Here i tary coproporphyria is cause ue to eficiency of (A) Protoporphyrinogen oxi as e (B) ALA synthase (C) ALA ehy ratase (D) Coproporphyrinogen oxi ase 61. The en zyme involve in variegate porphyria is (A) Protoporphyrinogen oxi ase (B) Copro porphyrinogen oxi ase (C) Uroporphyrinogen ecarboxylase (D) ALA ecarboxylase 6 2. Protoporphyria (erythrohepatic) is characterize by the eficiency of (A) ALA synthase (B) ALA hy ratase (C) Protophyrinogen oxi ae (D) Ferrochelatase 63. Th e amount of coproporphyrins excrete per ay in feces is about (A) 10–50 µgs (C) 200–2 50 µgs (B) 100–150 µgs (D) 300–1000 µgs 66. All immunoglobulins contain (A) (B) (C) (D) 4 L chains 4 H chains 3 L chains 2 L chains an 2 H chains 185 67. An immunoglobulin molecule always contains (A) (B) (C) (D) 1 κ and 3 λ type of c hains 2 κ and 2 λ type of chains 3 κ and 1λ type of chains 2 κ and 2 λ chains
68 . The number of types of H chains identified in human is (A) 2 (C) 4 (B) 3 (D ) 5 69. The number of hypervariab e region in L chain is (A) 1 (C) 3 (B) 2 (D) 4 70. The number of hypervariab e region in H chain is (A) 1 (C) 3 (A) Ig G (C) Ig M (A) Ig E (C) Ig M (A) Ig G (C) Ig M (A) Ig G (C) Ig M (A) Ig A (C) Ig D (A) I g D (C) Ig G (B) 2 (D) 4 (B) Ig A (D) Ig D (B) Ig A (D) Ig D (B) Ig A (D) Ig D ( B) Ig A (D) Ig D (B) Ig M (D) Ig E (B) Ig M (D) Ig E 71. Type γ H chain is present in 72. Type α H ch in is present in 64. The immunoglo ulins re differenti ted nd lso n med on the sis of (A) El ectrophoretic mo ility (B) He t st ility (C) Molecul r weight (D) Sediment iton coefficient like 7 S, 19 S etc. 65. The immunoglo ulins re cl ssified on the sis of (A) Light ch ins (B) He vy ch ins (C) C r ohydr te content (D) Electroph oretic mo ility 73. Type µ H ch in is present in 74. Type δ H chain is present in 75. Type ε H chain is pr s nt in
76. A ‘J’ chain is pr s nt in
186 77. A s cr tory prot in T chain (T prot in) is pr s nt in (A) Ig A (C) Ig D (B) Ig M (D) Ig E MCQs IN BIOCHEMISTRY 85. Th immunoglobulin which can cross th M (D) Ig D
plac nta is (A) Ig A (C) Ig G (B) Ig
78. A p ntam r immunoglobulin is (A) Ig G (C) Ig M (B) Ig A (D) Ig E 86. Th immunoglobulin poss ssing low st conc ntration of carbohydrat A (C) Ig M (B) Ig E (D) Ig G
is (A) Ig
79. Th portion of th immunoglobulin mol cul that binds th sp cific antig n i s form d by (A) (B) (C) (D) Variabl r gions of H and L chains Constant r gion o f H chain Constant r gion of L chain Hing r gion 87. Th 00 mg%
normal s rum l v l of Ig G is (A) 1200 mg% (C) 300 mg% (B) 500 mg% (D) 2
88. Th
half lif
of Ig G is (A) 2–8 days (C) 19–24 days (B) 1–4 days (D) 6 days
80. Th class sp cific function of th diff r nt immunoglobulin mol cul s is con stitut d by (A) (B) (C) (D) Variabl r gion of L chain Constant r gion of H chai n Variabl r gion of H chain Constant r gion particularly CH2 and CH3 of H chain 89. Most h at labil
immunoglobulin is (A) Ig G (C) Ig M (B) Ig A (D) Ig D
90. Th immunoglobulin poss ssing high st conc ntration of carbohydrat g G (C) Ig A (B) Ig M (D) Ig D 81. Hing r gion, th r gion of Ig mol cul which is o nzym s is th (A) R gion b tw n first and s cond (domains CH1 and CH2) (B) R gion b tw n s cond and chain (CH2 and CH3) (C) Variabl r gions of H chain hain 82. Th small st immunoglobulin is (A) Ig G (C)
is (A) I
fl xibl and mor xpos d t constant r gions of H chain third constant r gions of H (D) Variabl r gions of L c Ig D (B) Ig E (D) Ig A
91. Th
normal s rum l v l of Ig D is (A) 1 mg% (C) 3 mg% (B) 2 mg% (D) 5 mg%
92. Th
half lif
93. Th 2%
carbohydrat
83. Th
numb r of sub class s of Ig G is (A) 2 (C) 4 (B) 3 (D) 8
of Ig D is (A) 1 day (C) 10–15 days (B) 2–8 days (D) 20–24 days cont nt of Ig M is about (A) 2.8% (C) 8.0% (B) 6.4% (D) 10.
94. Th immunoglobulin having high st s dim ntation co ffici nt is (A) Ig G (C) Ig M (B) Ig A (D) Ig D 84. Most abundant Ig G subclass in th ) Ig G4
s rum is (A) Ig G1 (C) Ig G3 (B) Ig G2 (D
95. Th immunoglobulin having high st mol cular w ight is (A) Ig G (C) Ig E (B) Ig M (D) Ig A
MINERAL METABOLISM 96. Th half lif of Ig M is (A) 2 days (C) 5 days (B) 4 days (D) 8 days 187 107. Th normal s rum l v l of phosphorus in human adult is (A) 1–2 mg (C) 3–4.5 mg (B) 2–3 mg (D) 5–7 mg 97. Th mg%
normal s rum l v l of Ig M is (A) 50 mg% (C) 200 mg% (B) 120 mg% (D) 300
98. Th immunoglobulin associat d with r ginic antibody is (A) Ig E (C) Ig M (B) Ig D (D) Ig A 108. An incr as in carbohydrat m tabolism is accompani d by t mporary d cr as in s rum: (A) Calcium (C) Iron (B) Phosphat (D) Sodium 99. Th immunoglobulin having l ast conc ntration in s rum is (A) Ig A (C) Ig D (B) Ig M (D) Ig E 109. In rick ts of th common low-phosphat vari ty, s rum phosphat valu s may go as low as (A) 1–2 mg/100 ml (B) 2–3 mg/100 ml (C) 3–4 mg/100 ml (D) 4–5 mg/100 ml 110 . Th normal s rum l v l of phosphorous in childr n vari s from (A) 1–2 mg/100 ml (B) 2–3 mg/100 ml (C) 3–4 mg/100 ml (D) 4–7 mg/100 ml 111. An inh rit d or acquir d r nal tubular d f ct in th r absorption of phosphat (Vit D r sistant rick t) is charact riz d with (A) Normal s rum Phosphat (B) High s rum phosphat (C) A low blood phosphorous with l vat d alkalin Phosphat (D) A high blood phosphorous with d cr as d alkalin phosphatas 112. Th total magn sium cont nt in gms of human body is about (A) 5 (C) 15 (B) 10 (D) 21 100. Th ays
half lif
of Ig E prot in is (A) 1–6 days (C) 10 days (B) 2–8 days (D) 20 d
101. Th immunoglobulin which provid s high st antiviral activity is (A) Ig D (C ) Ig A (B) Ig E (D) Ig G 102. Th
half lif
of Ig A is (A) 6 days (C) 5–10 days (B) 2–4 days (D) 12–20 days
103. Th 00 mg%
normal s rum l v l of Ig A is (A) 100 mg% (C) 300 mg% (B) 200 mg% (D) 4
104. Calcium is xcr t d by (A) (B) (C) (D) Kidn y Kidn y and int stin nd liv r Kidn y and pancr as 113. Iron is a compon nt of (A) H moglobin (C) Transf ras Transaminas
Kidn y a
(B) C ruloplasmin (D)
105. A d cr as in th ioniz d fraction of s rum calcium caus s (A) T tany (C) O st omalacia (B) Rick ts (D) Ost oporosis 114. Daily r quir m nt of iron for normal adult mal (B) 10 mg (D) 20 mg 106. A ris in blood calcium may indicat Rick ts (D) Hyp rvitaminosis D
is about (A) 5 mg (C) 15 mg
(A) Pag t’s dis as
(C) Ost omalacia (B)
188 115. Th normal cont nt of prot in bound iron (PBI) in th plasma of mal s is (A ) (B) (C) (D) 120–140 µg/100 ml 200–300 µg/100 ml 120–140 µg/100 ml 200–300 µg/100 ml Th bound iron is low Th plasma bound iron is high Total iron binding capacity is low Both th plasma bound iron and total iron binding capacity ar low MCQs IN BIOCHEMISTRY 123. Th b st sourc of iron is (A) Organ m ats (C) Tomato (B) Milk (D) Potato 124. An incr as d s rum iron and d cr as d iron binding capacity is found in (A) (B) (C) (D) F d fici ncy an mia Sid roblastic an mia Folat d fici ncy an mia Sickl c ll an mia 116. In iron d fici ncy an mia (A) (B) (C) (D) 125. Th absorption of iron is incr as d 2–10 tim s of normal in (A) (B) (C) (D) ( A) (B) (C) (D) Iron d fici ncy an mia Pr gnancy Sph rocytosis Sickl c ll an mia Stomach and duod num Il um Ca cum Colon (B) Catalas (D) P roxidas 117. Th total iron cont nt of th human body is (A) 400–500 mg (C) 2–3 g 118. In h patic dis as s (A) Both th bound iron and total iron binding capacity of th pl asma may b low (B) Both th bound iron and total iron binding capacity of th p lasma may b high (C) Only bound iron may b high (D) Only th total iron bindin g capacity may b high 119. Th r comm nd d daily r quir m nt of iron for wom n of 18–55 yrs ag is (A) 5 mg (C) 10 mg (B) 8 mg (D) 15 mg (B) 1–2 g (D) 4–5 g 126. Iron is mainly absorb d from 127. Th iron containing nonporphyrin is (A) H mosid rin (C) Cytochrom C 128. M ol cular iron is (A) (B) (C) (D) Stor d primarily in th spl n Exr t d in th u rin as F 2+ Stor d in th body in combination with f rritin Absorb d in th f r ric form 120. Th p rc nt of total iron in body in h moglobin is (A) 10–20 (C) 30–40 (B) 20–30 (D) 60–70 129. In h mochromatosis, th B) Copp r (D) Fats
liv r is infiltrat d with (A) Iron (C) Molybd num (
121. A hypochromic microcytic an mia with incr as d iron stor s in th bon marr ow may b (A) (B) (C) (D) Iron r sponsiv Pyridoxin r sponsiv Vitamin B12 r sp onsiv Folat r sponsiv (B) Milk (D) Potato 130. An acquir d sid rosis-Bantu sid rosis is du to (A) (B) (C) (D) Foods cook d in iron pots Di t high in phosphorous Di t high in calcium High fat di t 122. A good sourc
of iron is (A) Spinach (C) Tomato
131. Th amount of copp r in th mg (D) 500–1000 mg
human body is (A) 50–80 mg (C) 400–500 mg (B) 100–150
MINERAL METABOLISM 132. Th amount of copp r in muscl s is about (A) 10 mg (C) 64 mg (A) 5 mg (C) 1 5 mg (B) 30 mg (D) 100 mg (B) 10 mg (D) 23 mg 189 142. M nk ’s dis as is du to an abnormality in th gn sium (B) Mangan s (D) Copp r 133. Th
m tabolism of (A) Iron (C) Ma
amount of copp r in bon s is about
143. M nk ’s dis as (Kinky or st l hair dis as ) is a X-link d dis as charact r iz d by (A) High l v ls of plasma copp r (B) High l v ls of c ruloplasmin (C) Lo w l v ls of plasma copp r and of c uloplasmin (D) High l v l of h patic copp r 1 44. Th trac l m nt catalyzing h moglobin synth sis is (A) Mangan s (C) Copp r (B) Magn sium (D) S l nium 134. Th normal s rum of conc ntration of copp r in mg/100 ml vari s b tw 0–5 (C) 100–200 (B) 50–100 (D) 200–300
n (A)
135. Th normal s rum conc ntration of c ruloplasmin in mg/100 ml vari s b tw (A) 5–10 (C) 25–43 (B) 10–20 (D) 50–100
n
136. R comm nd d daily di tary r quir m nt of copp r for adults is (A) 0.5–1 mg (C ) 3.5–4.5 mg (A) (B) (C) (D) (B) 1.5–3.0 mg (D) 4.5–5.5 mg 145. Th 10 mg
total body cont nt of mangan s
137. Th
rich st sourc
146. In blood th ) 2–4 (D) 4–20
is about (A) 2 mg (C) 8 mg (B) 4 mg (D)
of copp r is Liv r Milk L gum s Gr
valu s of mangan s
138. Th cytosolic sup roxid u2+ and Zn2+ (D) Mn 2+
n l afy v g tabl s
in µg / 100 ml vari s b tw
dismutas
nzym
n (A) 0–4 (C) 3–5 (B
contains (A) Cu2+ (C) Zn 2+ (B) C
147. Th ad quat daily di tary r quir m nt of mangan s B) 2–5 mg (D) 10–20 mg
is (A) 1–2 mg (C) 5–10 mg (
139. Th d fici ncy of copp r d cr as s th activity of th nzym : (A) Lysin xidas (C) Tyrosin oxidas (B) Lysin hydroxylas (D) Prolin hydroxylas 148. Mitochondrial sup roxid r (D) Mangan s 140. Wilson’s dis Wilson’s dis as v l in plasma is on of copp r is d
dismutas
o
contains (A) Zinc (C) Magn sium (B) Copp
as is a condition of toxicosis of (A) Iron (C) Chromium 141. In (A) (B) (C) (D) Copp r fails to b xcr t d in th bil Copp r l d cr as d C ruloplasmin l v l is incr as d Int stinal absorpti cr as d (B) Copp r (D) Molybd num
149. Mitochondrial pyruvat (D) Magn sium
carboxylas
contains (A) Zinc (C) Mangan s
(B) Zinc
150. Th ad quat daily di tary r quir m nt of molybd num for normal human adult is (A) 10–20 µg (C) 50–70 µg (B) 25–50 µg (D) 75–200 µg
190 151. In human b ings molybd num is mainly absorb d from (A) Liv r (C) Int stin (B) Kidn y (D) Pancr as MCQs IN BIOCHEMISTRY 161. Total body cont nt of s l nium is about (A) 1–2 mg (C) 4–10 mg (A) 5 µg /100 ml ( C) 10 µg /100 ml (B) 2–4 mg (D) 50–100 mg (B) 8 µg /100 ml (D) 13 µg /100 ml 162. Normal s rum l v l of s l nium is 152. In human b ings molybd num is mainly at (D) T ars
xcr t d in (A) F c s (C) Urin
(B) Sw
163. S l nium is a constitu nt of th nzym : (A) Glutathion p roxidas (B) Hom og ntisat oxidas (C) Tyrosin hydroxylas (D) Ph nylalanin hydroxylas 164. A nonsp cific intrac llular antioxidant is (A) Chromium (C) S l nium (B) Magn sium (D) Nick l 153. Molybd num is a constitu nt of (A) Hydroxylas s (C) Transaminas s (B) Oxida s s (D) Transf ras s 154. Saf and ad quat daily di tary intak of chromium in adults in mg is (A) 0 .01–0.02 (C) 0.03–0.04 (B) 0.02–0.03 (D) 0.05–0.2 155. Rich st sourc of chromium is (A) Br w r’s y as (B) Milk and milk products ( C) Y llow v g tabl s (D) Gr n v g tabl s 156. M tallic constitu nt of “Glucos to l ranc factor” is (A) Sulphur (C) Chromium (B) Cobalt (D) S l nium 165. Cobalt forms an int gral part of th lat
vitamin: (A) B1 (C) B 12 (B) B6 (D) Fo
166. Cobalt may act as cofactor for th nzym : (A) Glycl-glycin dip ptidas (B ) Elastas (C) Polynucl otidas s (D) Phosphatas 167. Exc ss intak of cobalt fo r long r p riods l ads to (A) Polycyth mia (B) M galoblastic an mia (C) P rnicio us an mia (D) Microcytic an mia 168. Th total sulphur cont nt of th body is (A ) 25–50 gm (C) 100–125 gm (B) 50–75 gm (D) 150–200 gm 157. Int stinal absorption of chromium is shar d with (A) Mn (C) Ca (B) Mg (D) Z n 158. S rum l v l of chromium in h althy adult is about (A) 2-5 µg/100 ml (B) 6-20 µg /100 ml (C) 30-60 µg/100 ml (D) 50-100 µg/100 ml 159. Chromium is pot ntiator of (A) Insulin (C) Thyroxin (B) Glucagon (D) Parathromon 169. Sulphur is mad availabl to th body by th amino acids: (A) Cystin and m thionin (B) Taurin and alanin (C) Prolin and hydroxyprolin (D) Arginin an d lysin 160. R comm nd d daily di tary allowanc 20 (C) 50 (B) 40 (D) 70
of s l nium for adult human in µg is (A)
MINERAL METABOLISM 170. Sulphur containing co nzym is (A) (B) (C) (D) (A) (B) (C) (D) NAD FAD Pyri doxal phosphat Biotin Thyroid gland as thyroglobulin Liv r Int stin Skin (B) P TH (D) Adr nalin 179. D ntal cari s occur du to 191 (A) Drinking wat r containing l ss than 0.2 ppm of fluorin ontaining gr at r than 1.2 ppm of fluorin (C) Drinking wat lcium (D) Drinking wat r containing h avy m tals 180. Total n body is about (A) 800 mg (C) 2000 mg (B) 1200 mg (D) 3200 171. Iodin
is stor d in
172. Iodin
is th
(B) Drinking wat r c r containing high ca zinc cont nt of huma mg
constitu nt of (A) T3 and T4 (C) Insulin
181. M tal r quir d for polym rization of insulin is (A) Copp r (C) Cobalt (B) C hromium (D) Zinc 173. Goitrog nic substanc pr s nt in cabbag is (A) 5-vinyl-2 thio oxalzolidon (B) Pyridin -3-carboxylic acid (C) 3-Hydroxy-4, 5-dihydroxym thyl1–2-m thyl pyrid in (D) δ-ALA ehy ratase 174. For an a ult male aily requirement of io ine is (A ) 25–50 µg (C) 100–150 µg (B) 50–100 µg (D) 200–250 µg 182. Metalloenzyme-retinene for polymerization of insulin is (A) Copper (C) Coba lt (B) Zinc (D) Manganese 183. An important zinc containing enzyme is (A) (B) (C) (D) Carboxypepti ase A I socitrate ehy rogenase Cholinesterate Lipoprotein lipase 175. Recommen e aily intake of fluori e for a normal a ult is (A) 1.5–4.0 mg (C) 5–10 mg (B) 0–1 mg (D) 10–20 mg 184. Acro ermatitis enteropathica is e (C) Io ine (B) Molyb enum (D) Zinc
ue to
efective absorption of (A) Manganes
176. The percentage of fluori e present in normal bone is (A) 0.01–0.03 (C) 0.10–0.1 2 (B) 0.04–0.08 (D) 0.15–0.2 185. Hypogona ism (D) Manganese
evelops
ue to
eficiency of (A) Sulphur (C) Zinc (B) Cobalt
177. The percentage of fluori e present in .20 (B) 0.05–0.10 (D) 0.20–0.40
ental enamel is (A) 0.01–0.02 (C) 0.15–0
186. Psychotic symptoms an parkinsonism like symptoms evelop ue to inhalation poisoning of (A) Manganese (C) Magnesium (B) Phosphorous (D) Zinc 178. Fluorosis occurs ue to (A) Drinking water containing less than 0.2 ppm of fluorine (B) Drinking water containing high calcium (C) Drinking water containin g greater than 1.2 ppm of fluroine (D) Drinking water containing heavy metals 187. One gram of carbohy rate on complete oxi ation in the bo y yiel s about (A) 1 Kcal (C) 6 Kcal (B) 4 Kcal (D) 9 Kcal
192 188. One gram of fat on complete oxi ation in the bo y yiel s about (A) 4 Kcal ( C) 9 Kcal (B) 6 Kcal (D) 12 Kcal MCQs IN BIOCHEMISTRY 199. B.M.R. is subnormal in (A) (B) (C) (D) A ng’s syn rome Fever
ison’s
isease A renal tumour Cushi
189. One gram of protein on complete oxi ation in the bo y yiel s about (A) 2 Kc al (C) 8 Kcal (B) 4 Kcal (D) 12 Kcal 190. R.Q. of mixe iet is about (A) 0.70 (C) 0.85 (A) 0.70 (C) 0.80 192. (A) 0. 75 (C) 0.85 (A) 0.75 (C) 0.85 (A) 5% (C) 20% (B) 0.80 (D) 1.0 (B) 0.75 (D) 0.85 (B) 0.80 (D) 1.0 (B) 0.80 (D) 1.0 (B) 10% (D) 30% 200. A healthy 70 kg man eats a well balance iet containing a equate calories an 62.5 g of high quality protein per ay. Measure in grams of nitrogen, his aily nitrogen balance woul be (A) +10 g (C) 0 g (B) +6.25 g (D) –6.25 g 191. R.Q. of proteins is about 201. The percentage of nitrogen retaine in the bo y after absorption of iet re presents (A) (B) (C) (D) Digestibility coefficient of proteins Biological value of proteins Protein efficiency ratio Net protein utilisation R.Q. of carbohy rates is about 193. R.Q. of fats is about 202. In a person increase in weight in gms per gm of protein consumption represe nts (A) (B) (C) (D) Protein efficiency ratio Digestibility value of proteins Bio logical value of proteins Net protein utilisation 194. Proteins have the SDA: 203. The percentage of foo nitrogen that is retaine in the bo y represents (A) (B) (C) (D) Digestibility coefficient Biological value of proteins Protein effi ciency ratio Net protein utilisation 195. Humans most easily tolerate a lack of the nutrient: (A) Protein (C) Io ine (B) Lipi (D) Carbohy rate 196. The basal metabolic rate (B.M.R.) is measurement of (A) (B) (C) (D) Energy expen iture uring sleep Energy expen iture after 100 m walk Energy expen iture after a meal Energy expen iture un er certain basal (Stan ar ) con itions (B) St arvation (D) Hypothyroi ism (B) Leukemia (D) Hyperthyroi ism 204. The chemical score of ifferent proteins is calculate in terms of (A) Egg proteins (C) Fish proteins (A) 94 (C) 51 (A) 95 (C) 71 (A) 86 (C) 64 (B) Milk pr oteins (D) Wheat proteins (B) 60 (D) 40 (B) 60 (D) 67 (B) 71 (D) 54 205. Biological value of egg protein is 197. B.M.R. is raise (C) Car iac failure
in (A) Polycythemia (C) Lipi
206. Biological value of protein of cow’s milk is 198. B.M.R. is lowere
in
nephrosis (A) Hypothyroi ism
207. Biological value of soyabean protein is
MINERAL METABOLISM 208. Plasma bicarbonate is ecrease in (A) (B) (C) (D) (A) (B) (C) (D) 210. Res piratory alkalosis Respiratory aci osis Metabolic alkalosis Metabolic aci osis R espiratory alkalosis Metabolic alkalosis Respiratory aci osis Metabolic aci osis 216. Respiratory aci osis occurs in 193 (A) Any isease which impairs respiration like emphysema (B) Renal isease (C) P oisoning by an aci (D) Pyloric stenosis 217. Metabolic alkalosis occurs (A) As consequence of high intestinal obstruction (B) In central nervous system isease (C) In iarrhoea (D) In colitis 218. Respiratory alkalosis occurs in (A) Hyster ical hyperventilation (B) Depression of respiratory centre (C) Renal iseases (D ) Loss of intestinal flui s 219. Morphine poisoning causes (A) Metabolic aci osi s (B) Respiratory aci osis (C) Metabolic alkalosis (D) Respiratory alkalosis 220 . Salicylate poisoning in early stages causes (A) Metabolic aci osis (B) Respira tory aci osis (C) Metabolic alkalosis (D) Respiratory alkalosis 221. The compoun having the lowest re ox potential amongst the following is (A) Hy rogen (C) Cy tochrome b (B) NAD (D) Cytochrome a 209. Plasma bicarbonate is increase
in
Total CO2 is increase in (A) Respiratory aci osis (B) Metabolic alkalosis (C) B oth respiratory aci osis an metabolic alkalosis (D) Respiratory alkalosis 211. Respiratory aci osis is cause by (A) Increase in carbonic aci relative to bicarbonate (B) Decrease in bicarbonate fraction (C) Increase in bicarbonate fr action (E) Decrease in the carbonic aci fraction 212. Respiratory alkalosis is cause by (A) (B) (C) (D) (A) (B) (C) (D) (A) (B) (C) (D) (A) (B) (C) (D) An inc rease in carbonic aci fraction A ecrease in bicarbonic fraction A ecrease in the carbonic aci fraction An increase in bicarbonate fraction Metabolic alkalos is Respiratory alkalosis Metabolic aci osis Respiratory aci osis Uncontrolle i abetes with ketosis Pneumonia Intestinal Obstruction Hepatic coma Pneumonia Prol onge starvation Intestinal obstruction Bulbar polio 213. Meningitis an
encephalitis cause
214. Metabolic aci osis is cause
in
222. All the oxi ases contain a metal which is (A) Copper (C) Manganese (B) FAD (D) None of these 223. Isocitrate ehy rogenases is (A) Aerobic ogenase (C) Hy roperoxi ase (D) Oxygenase 215. Metabolic aci osis is cause
in
ehy rogenase (B) Anaerobic
ehy r
194 224. Iron-pophyrin is present as prosthetic group in (A) Cytochromes (C) Peroxi ase (B) Catalases (D) None of these 234. MCQs IN BIOCHEMISTRY The porphyrin present in haem is (A) Uroporphyrin (C) Coproporphyrin (B) Protopo rphyrin I (D) Protoporphyrin II 225. Microsomal hy roxylase system contains a (A) Di-oxygenase (C) Both (A) an (B) (B) Mono-oxygenase (D) None of thse 235. An amino aci require ) Glycine (D) Histi ine
for porphyrin synthesis is (A) Proline (C) Serine (B
226. Superoxi e ra icals can be etoxifie (B) Cytochrome b (D) None of these
by (A) Cytochrome c (C) Cytochrome a
236. Which of the following coenzyme is require for porphyrin synthesis? (A) (B ) (C) (D) Coenzyme A Pyri oxal phosphate Both (A) an (B) None of these 227. A copper containing cytochrome is (A) Cytochrome a (C) Cytochrome a3 (B) Cy tochrome P-450 (D) None of these 228. Rate of tissue respiration is raise when the intracellular concentration o f (A) ADP increases (C) ADP ecreases (B) ATP increases (D) None of these 237. The regulatory enzyme for haem synthesis is (A) (B) (C) (D) 238. ALA synthe tase haem synthetase Both (A) an (B) None of these 229. Which of the following component of respiratory chain is not attache to th e inner mitochon rial membrane? (A) Coenzyme Q (C) Both (A) an (B) (B) Cytochro me c (D) None of these Regulation of haem synthesis occurs by (A) (B) (C) (D) Covalent mo ification Rep ression - erepression In uction Allosteric regulation 230. In some reactions, energy is capture B) UTP (D) None of these
in the form of (A) GTP (C) CTP 231. (
239. Sigmoi al oxygen issociation curve is a property of (A) (B) (C) (D) Haemog lobin Carboxyhaemoglobin Myoglobin Methaemoglobin Substrate-linke phosphorylation occurs in (A) Glycolytic pathway (B) Citric aci cycle (C) Both (A) an (B) (D) None of these 232. Hy rogen peroxi e may be etoxifie in the absence of an oxygen acceptor by (A) Peroxi ase (C) Both (A) an (B) (B) Catalase (D) None of these 240. Cyanmethaemoglobin can be forme (D) All of these
from (A) Oxy Hb (C) Carboxy Hb (B) Met Hb
233. Superoxi e ra icals can be etoxifie by (A) (B) (C) (D) Cytochrome c Super oxi e ismutase Both (A) an (B) None of these 241. In thalassemia, an amino aci is substitute Beta chain Alpha an beta chains Any chain
in (A) (B) (C) (D) Alpha chain
MINERAL METABOLISM 242. Haem synthetase is congenitally porphyria (B) Protoporphyria (C) Here ia 243. During break own of haem, the pyrrole rings is broken: (A) I an II
eficient in (A) Congenital erythropoietic itary coproporphyria (D) Variegate porphyr methenyl bri ge between the following two (C) III an IV (B) II an III (D) IV an I
195 249. Bilirubin UDP-glucuronyl transferase is absent from liver in (A) (B) (C) (D ) Crigler-Najjar syn rome, type I Gilbert’s isease Crigler-Najjar syn rome, type II Rotor’s syn rome 250. Unconjugate is (D) Methanal
bilirubin in serum is soluble in (A) Water (C) Aci s (B) Alkal
244. Pre- hepatic jaun ice occurs because of (A) Increase haemolysis (B) Liver amage (C) Biliary obstruction (D) None of these 245. kernicterus can occur in ( A) Haemolytic jaun ice (B) Hepatic jaun ice (C) Obstructive jaun ice (D) All of these 246. Bile pigments are not present in urine in (A) Haemolytic jaun ice (B) Hepatic jaun ice (C) Obstructive jaun ice (D) Rotor’s syn rome 247. Serum alkalin e phosphatase is greatly increase in (A) Haemolytic jaun ice (B) Hepatic jaun i ce (C) Obstructive jaun ice (D) None of these 248. The active transport system f or hepatic uptake of bilirubin is congenitally efective in (A) Gilbert’s isease (B) Crigler-Najjar syn rome (C) Rotor’s syn rome (D) Dubin-Johnson syn rome 251. Excretion of conjugate bilirubin from liver cells into biliary canaliculi is efective in (A) (B) (C) (D) Gilbert’s isease Crigler-Najjar syn rome Lucey-Dr iscoll syn rome Rotor’s syn rome 252. Break own of 1gm haemoglobin pro uces (A) 20 mg of bilirubin (B) 35 mg of b ilirubin (C) 50 mg of bilirubin (D) 70 mg of bilirubin 253. Variable regions are present in (A) (B) (C) (D) Immunoglobulins α-Ch ins of T cell receptors β-Ch ins of T cell receptors All of these
254. The tot l mount of c lcium in 1 kg (B) 500 gm (D) 10 kg
n
ver ge
255. The following proportion of the tot l teeth: (A) 75% (C) 95% (B) 90% (D) 99% 256. The norm l r nge of pl sm mg/dl (D) 11-15 mg/dl
dult m n is
out (A) 100 gm (C)
ody c lcium is present in
ones
nd
c lcium is (A) 3-5 mg/dl (C) 9-11 mg/dl (B) 5-10
257. Which of the norm l r nge of ionized c lcium in pl sm 4-5 mg/dl (B) 2-4 mEq/L (D) 4-5 mEq/L
is (A) 2-4 mg/dl (C)
196 258. Tet ny c n occur in (A) (B) (C) (D) Hypoc lc emi Hypoc lc emi nd lk losis
Hyperc lc emi
Alk losis
MCQs IN BIOCHEMISTRY 266. Hypoc lc emi c n occur in ll the following except (A) (B) (C) (D) Rickets Osteom l ci Hyperp r thyroidism Intestin l m l sorption 259. Intestin l sorption of c lcium occurs y (A) (B) (C) (D) Active t keup Si mple diffusion F cilit ted diffusion Endocytosis 267. The m jor c lcium s lt in ones is (A) (B) (C) (D) C lcium c r on te C lciu m chloride C lcium hydroxide C lcium phosph te 260. Intestin l sorption of c lcium is h mpered (B) Phyt te (D) L ctose
y (A) Phosph te (C) Proteins
268. The correct st tement out serum inorg nic phosphorous concentr tion is (A ) (B) (C) (D) It is higher in men th n in women It is higher in women th n in me n It is higher in dults th n in children It is higher in children th n in dult s 261. C lcitriol f cilit tes c lcium sorption y incre sing the synthesis of th e following in intestin l mucos : (A) (B) (C) (D) C lcium Binding Protein Alk li ne Phosph t se C lcium-dependent ATP se All of these 269. The product of serum c lcium concentr tion (mg/dl) nd serum inorg nic phos phorous concentr tion (mg/dl) in dults is out (A) 30 (C) 50 (B) 40 (D) 60 262. A A) (B) n of p retion
high pl sm c lcium level decre ses intestin l sorption of c lcium y ( (C) (D) Stimul ting the secretion of p r thormone Inhi iting the secretio r thormone Decre sing the synthesis of cholec lciferol Inhi iting the sec of thyroc lcitonin
270. The product of serum c lcium concentr tion (mg/dl) nd serum inorg nic phos phorous concentr tion (mg/dl) in children is out (A) 30 (C) 50 (B) 40 (D) 60 263. The d ily c lcium requirement of g (B) 600 mg (D) 1,000 mg
n
dult m n is
out (A) 400 mg (C) 800 m
271. The product of serum c lcium concentr tion (mg/dl) nd serum inorg nic phos phorous concentr tion (mg/dl) is decre sed in (A) (B) (C) (D) Rickets Hypop r th yroidism Hyperp r thyroidism Ren l f ilure 264. The d ily c lcium requirement in pregn ncy g (C) 1,200 mg (B) 800 mg (D) 1,500 mg
nd l ct tion is
out (A) 600 m
265. Hyperc lc emi c n occur in ll the following except (A) (B) (C) (D) Hyperp r thyroidism Hypervit minosis D Milk lk li syndrome Nephrotic syndrome 272. Serum inorg nic phosphorous rises in ll the following conditions except (A ) (B) (C) (D) Hypop r thyroidism Hypervit minosis D Chronic ren l f ilure After c r ohydr te-rich me l
MINERAL METABOLISM 273. Serum inorg nic phosphorous decre ses in ll the following conditions excep t (A) (B) (C) (D) Hyperp r thyroidism Intestin l m l sorption Osteom l ci Chro nic ren l f ilure 282. Serum pot ssium level decre ses in (A) (B) (C) (D) F mili l periodic p r lysis Addison’s dise se Ren l f ilure All of these 197 274. Serum m gnesium level r nges ) 9–11 mg/dl
etween (A) 2–3 mg/dl (C) 6–8 mg/dl (B) 3–5 mg/dl (D
283. Concentr tion of the following is higher in intr cellul r fluid th n in ext r cellul r fluid: (A) Sodium (C) Chloride (B) Pot ssium (D) Bic r on te 275. M gnesium ions FAD (D) CoA
re required in the re ctions involving (A) NAD (C) ATP (B)
284. Norm l r nge of serum pot ssium is (A) 2.1-3.4 mEq/L (C) 5.4–7.4 mEq/L (B) 3. 5-5.3 mEq/L (D) 7.5–9.5 mEq/L 276. Norm l r nge of serum sodium is (A) 30–70 mEq/L (B) 70–110 mEq/L (C) 117–135 mEq/ L (D) 136–145 mEq/L 277. Sodium is involved in the ctive upt ke of (A) D-Glucose (C) L-Amino cids (B) D-G l ctose (D) All of these 285. Norm l r nge of serum chloride is (A) 24–27 mEq/L (B) 70–80 mEq/L (C) 100–106 mEq /L (D) 120–140 mEq/L 286. An extr cellul r fluid h ving higher concentr tion of chloride th n serum is (A) Bile (C) CSF (B) Swe t (D) P ncre tic juice 278. Aldosterone incre ses re sorption of sodium in (A) (B) (C) (D) Proxim l co nvoluted tu ules Ascending lim of loop of Henle Descending lim of loop of Henl e Dist l convoluted tu ules 287 Tot l 6–7 gm
mount of iron in
n
dult m n is
out (A) 1–2 gm (C) 3–4 gm (B) 2–3 gm (D)
288. H emoglo in cont ins out (A) (B) (C) (D) 30% of the tot l ody iron 50% o f the tot l ody iron 75% of the tot l ody iron 90% of the tot l ody iron 279. Restriction of sodium int ke is commonly dvised in (A) Addison’s dise se (B) Di rrhoe (C) Hypertension (D) None of these 280. Serum sodium level rises in ll of the following except (A) (B) (C) (D) Ren l f ilure Prolonged steroid ther py Aldosteronism Dehydr tion 289. A out 5% of the tot l ody, iron is present in (A) Tr nsferrin (C) Cytochro mes (B) Myoglo in (D) H emosiderin 290. E ch h emoglo in molecule cont ins (A) One iron ) Two iron toms (D) Six iron toms
tom (C) Four iron
toms (B
281. Hypon tr emi occurs in the following condition: (A) Addison’s dise se (B) Ch ronic ren l f ilure (C) Severe di rrhoe (D) All of these 291. E ch myoglo in molecule cont ins (A) One iron Two iron toms (D) Six iron toms
tom (C) Four iron
toms (B)
198 292. Apoferritin molecule is m de up of (A) Four su units (C) Ten su units (B) E ight su units (D) Twenty-four su units MCQs IN BIOCHEMISTRY 302. D ily iron requirement of (D) 18 mg
n
dult m n is
out (A) 1 mg (C) 10 mg (B) 5 mg
293. Ferritin is present in (A) Intestin l mucos (B) Liver (C) Spleen (D) All o f these 294. Iron is stored in the form of (A) (B) (C) (D) Ferritin nd tr nsfer rin Tr nsferrin nd h emosiderin H emoglo in nd myoglo in Ferritin nd h emosid erin 303. D ily iron requirement of 10 mg (B) 2 mg (D) 20 mg 304. All the following t (D) Kidney
wom n of reproductive
ge is
out (A) 1 mg (C)
re good sources of iron except (A) Milk (C) Liver (B) Me
295. Iron is tr nsported in lood in the form of (A) Ferritin (C) Tr nsferrin (B ) H emosiderin (D) H emoglo in 305. Rel tively more iron is sor ed from (A) (B) (C) (D) Green le fy veget s Fruits Whole gr in cere ls Org n me ts
le
296. Molecul r weight of tr nsferrin is out (A) 40,000 (C) 80,000 (A) 50100 µg/d l (C) 50175 µg/dl (B) 60,000 (D) 1,00,000 (B) 100150 µg/dl (D) 250400 µg/dl 306. Iron 5–50 %
sorption from
297. Norm l pl sm
mixed diet is
out (A) 1–5 % (C) 20–25 % (B) 5–10 % (D) 2
iron level is
307. Iron deficiency c uses (A) (B) (C) (D) Normocytic Meg lo l stic n emi Pernicious n emi
n emi
Microcytic
n emi
298. Iron is present in ll the following except (A) Peroxid se (C) Aconit se (B ) X nthine oxid se (D) Fum r se 299. Tot l d ily iron loss of (D) 10 mg
n
dult m n is
out (A) 0.1 mg (C) 5 mg (B) 1 mg
308. Prolonged nd severe iron deficiency c n c use strophy of epithelium of (A ) Or l c vity (C) Stom ch (B) Oesoph gus (D) All of these 300. Iron sorption is h mpered ic cid (D) Amino cid
y (A) Ascor ic
cid (C) Phytic
cid (B) Succin
309. All of the following st tements out ronzed di etes re true except (A) (B) (C) (D) It is c used y excessive int ke of copper Skin ecomes pigmented Th ere is d m ge to β cells of Islets of L ngerh ns Liver is d m ged 301. Iron sorption is h mpered y (A) In chlorhydri (B) When ferritin conten t of intestin l mucos is low (C) When s tur tion of pl sm tr nsferring is low (D) When erythropoietic ctivity is incre sed 310. The tot l mount of iodine in the ) 45–50 mg (B) 20–25 mg (D) 75–100 mg
ody of
n
ver ge
dult is (A) 10–15 mg (C
MINERAL METABOLISM 311. Iodine content of thyroid gl nd in ) 4–8 mg (D) 25–30 mg
n
dult is
out (A) 1–3 mg (C) 10–15 mg (B
199 320. All the following st tements out Wilson’s dise se re correct except (A) It is genetic dise se (B) The defect involves copper-dependent P-type ATP se (C) Copper is deposited in liver, s l g ngli nd round corne (D) Pl sm copper level is incre sed in it 321. Which of the following st tements out Menke’s dis e se re true. (A) (B) (C) (D) It is n inherited disorder of copper met olism It occurs only in m les Pl sm copper is incre sed in it H ir ecomes steely nd kinky in it 312. D ily iodine requirement of 1 mg
n
dult is
out (A) 50 µg (C) 150 µg (B) 100 µg (D)
313. Consumption of iodised s lt is recommended in (A) (B) (C) (D) P tients with hyperthyroidism P tients with hypothyroidism Pregn nt women Goitre elt re s 314. All the following st tements out endemic goiter re true except (A) It oc curs in re s where soil nd w ter h ve low iodine content (B) It le ds to enl r gement of thyroid gl nd (C) It results ultim tely in hyperthyroidism (D) It c n e prevented y consumption of iodised s lt 315. The tot l mount of copper in t he ody of n ver ge dult is (A) 1 gm (C) 100 mg (A) 25–50 µg/dl (C) 100–200 µg/dl (B) 500 mg (D) 10 mg (B) 50–100 µg/dl (D) 200–400 µg/dl 322. The tot l mount of zinc in n ver ge dult is (A) 0.25–0.5 gm (C) 1.5–2.0 gm 323. Pl sm zinc level is (A) 10–50 µg/dl (C) 150–250 µg/dl (A) (B) (C) (D) (B) 50–150 µg/ (D) 250–500 µg/dl (B) 0.5–1.0 gm (D) 2.5–5.0 gm 324. Zinc is cof ctor for Acid phosph t se Alk line phosph t se Amyl se Lip se (B) Acetylcholine (D) Insulin 316. The norm l r nge of pl sm
copper is
325. Zinc is involved in stor ge
nd rele se of (A) Hist mine (C) Epinephrine
317. Copper deficiency c n c use (A) Polycyth emi (B) Leukocytopeni (C) Throm ocytopeni (D) Microcytic n emi 318. D ily requirement of copper in dults is out (A) 0.5 mg (C) 2.5 mg (B) 1 mg (D) 5 mg 326. Intestin l sorption of zinc is ret rded dmium (D) All of these 327. The d ily zinc requirement of ) (C) (D) (B) 10 mg (D) 25 mg
n
ver ge
y (A) C lcium (C) Phyt te (B) C dult is (A) 5 mg (C) 15 mg (A) (B
319. All the following st tements out cerulopl smin re correct except (A) (B) (C) (D) It is copper-cont ining protein It possesses oxid se ctivity It is s ynthesised in intestin l mucos Its pl sm level is decre sed inWilson’s dise se 328. Zinc deficiency occurs commonly in Acroderm titis enterop thic se Xeroderm pigmentosum Menke’s dise se
Wilson’s dise
200 329. Hypogon dism c n occur in deficiency of (A) Copper (C) Zinc (B) Chromium (D ) M ng nese MCQs IN BIOCHEMISTRY 340. 1 kc l is roughly equ l to (A) 4.2 J (C) 4.2 KJ (B) 42 J (D) 42 KJ 330. He ling of wounds m y ) Copper (D) Co lt
e imp ired in deficiency of (A) Selenium (C) Zinc (B
341. C lorific v lue of proteins s determined in om l/gm (C) 5.4 kc l/gm (B) 4.8 kc l/gm (D) 5.8 kc l/gm 331. Hypochromic microcytic er (D) None of these
n emi
c lorimeter is (A) 4 kc
c n occur in (A) Zinc (C) M ng nese (B) Copp
342. C lorific v lue of proteins in living person is less th n th t in om c lorimeter ec use (A) Digestion nd sorption of proteins is less th n 100% ( B) Respir tory quotient of proteins is less th n 1 (C) Specific dyn mic ction o f proteins is high (D) Proteins re not completely oxidized in living persons 34 3. C lorific v lue of lcohol is (A) 4 kc l/gm (C) 7 kc l/gm (B) 5.4 kc l/gm (D) 9 kc l/gm 332. The d ily requirement for m ng nese in 2–5 mg (D) 5–20 µg
dults is
out (A) 1–2 mg (C) 2–5 µg (B)
333. Moly denum is cof ctor for (A) X nthine oxid se (B) Aldehyde oxid se (C) Sulphite oxid se (D) All of these 334. A tr ce element h ving ntioxid nt functi on is (A) Selenium (C) Chromium (A) (B) (C) (D) (B) Tocopherol (D) Moly denum 344. Energy expenditure of person c n e me sured y (A) Bom c lorimetry (B) Direct c lorimetry (C) Indirect c lorimetry (D) Direct or indirect c lorimetry 3 45. Respir tory quotient of c r ohydr tes is out (A) 0.5 (C) 0.8 (B) 0.7 (D) 1 .0 335. Selenium is constituent of Glut thione reduct se Glut thione peroxid se C t l se Superoxide dismut se (B) Zinc (D) Vit min E (B) 0.8 ppm (D) 2 ppm 336. Selenium decre ses the requirement of (A) Copper (C) Vit min D (A) 0.4 ppm (C) 1.2 ppm 337. Upper s fe limit of fluorine in w ter is 346. Respir tory quotient of f ts is
out (A) 0.5 (C) 0.8 (B) 0.7 (D) 1.0
338. The d ily fluoride int ke should not exceed (A) 0.5 mg (C) 2 mg (A) (B) (C) (D) (B) 1 mg (D) 3 mg 347. Respir tory quotient of proteins is
out (A) 0.5 (C) 0.8 (B) 0.7 (D) 1.0
339. In dults, w ter constitutes out 50% of % of ody weight 75% of ody weight 348. Respir tory quotient of ) 0.7 (D) 0.85
n
ody weight 55% of
ver ge mixed diet is
ody weight 60
out (A) 0.65 (C) 0.75 (B
MINERAL METABOLISM 349. At respir tory quotient of 0.85, every litre of oxygen consumed represent s n energy expenditure of (A) 5.825 kc l (C) 3.825 kc l (A) (B) (C) (D) 351. (A ) (B) (C) (D) (A) (B) (C) (D) (B) 4.825 kc l (D) 2.825 kc l 201 359. All following ine (D) C dmium
re essenti l tr ce elements except (A) Iron (C) Zinc (B) Iod
350. BMR of he lthy dult men is out 30 kc l/hour/squ re metre 35 kc l/hour/sq u re metre 40 kc l/hour/squ re metre 45 kc l/hour/squ re metre 32 kc l/hour/squ re metre 36 kc l/hour/squ re metre 40 kc l/hour/squ re metre 44 kc l/hour/squ re metre Adults th n in children Men th n in women Veget ri ns th n in non-veget r i ns W rmer clim te th n in colder clim te (B) St rv tion (D) Fever 360. M ximum qu ntity of sodium is excreted through (A) Urine (C) Swe t (B) F ec es (D) None of these 361. All followings Eggs (D) C ge BMR of he lthy
re rich sources of m gnesium, except (A) Milk (C) Me t (B)
dult women is
362. All followings Pot toes (D) Liver
out
re poor sources of iron except (A) Milk (C) Whe t flour (B)
352. BMR is higher in 363. The Iron deficient children, sorption of Iron from GIT is (A) (B) (C) (D) Un ltered Dou le th n in norm l child M nifold th n in norm l child Lesser th n in norm l child 353. BMR is decre sed in (A) Pregn ncy (C) An emi 354. BMR is incre sed in (A) St rv tion (B) Hypothyroidism (C) Addison’s dise se (D) Pregn ncy 355. BMR is decr e sed in ll of the following except (A) Fever (C) St rv tion (B) Addison’s dise s e (D) Hypothyroidism 364. M in source of fluoride for hum n er (D) Eggs
eings is (A) Milk (C) Veget
365. Qu ntity of copper present in the g (B) 50–100 mg (D) 150–250 mg
ody of
n
les (B) W t
dult is (A) 0–50 mg (C) 100–150 m
356. BMR is incre sed in ll of the following except (A) Hyperthyroidism (B) An emi (C) Addison’s dise se (D) Pregn ncy 357. Specific dyn mic ction of c r ohydr tes is out (A) 5% (C) 20% (B) 13% (D) 30% 366. A level of 310–340 mg per 1000 ml of ot ssium (B) Iron (D) Sodium
lood is norm l for the (A) Copper (C) P
367. D ily requirement of phosphorous for B) 1.2 gms (D) 800–1200 mg
n inf nt is (A) 240–400 mg (C) 800 mg (
358. Specific dyn mic
ction of proteins is
out (A) 5% (C) 20% (B) 13% (D) 30%
368. M ximum qu ntity of Zinc is present in the ) Choroid (D) Bones
ody in (A) Prost te (C) Skin (B
202 369. Aver ge concentr tion of chloride ions in cere rospin l fluid per 100 ml is (A) 40 mg (C) 160 mg (B) 440 mg (D) 365 mg MCQs IN BIOCHEMISTRY 378. A deficiency of copper effects the form tion of norm l coll gen y reducing the ctivity of which of the following enzyme? (A) Prolyl hydroxyl se (B) Lysyl oxid se (C) Lysyl hydroxyl se (D) Glucosyl tr nsfer se 379. Molecul r iron (Fe) is (A) Stored prim rily in spleen (B) A sor ed in the intestine (C) A sor ed in the ferric, Fe+++ form (D) Stored in the ody in com in tion with ferritin 380. All the following st tements reg rding c lcium re correct except (A) It diffus es s div lent c tion (B) It freely diffuses cross the endopl smic reticulum of muscle cells (C) It c n exist in the lood s ionic form nd lso protein ou nd (D) It is found in high concentr tion in ones 381. Iron is sor ed from (A) Stom ch (B) Duodenum nd jejunum (C) Ileum (D) Noen of the ove 382. The norm l route of c lcium excretion is (A) Kidney (B) Kidney nd Liver (C) Kidney nd I ntestine (D) Kidney, Intestine nd P ncre s 383. Hypoc lc emi ffects (A) Skele t l muslces (B) Smooth muscles (C) C rdi c muscles (D) Skelet l muscles + smooth muscles + c rdi c muscles 384. Tr nsferrin is type of (A) Al umin (C) β1 glo ul in (B) α-glo ulin (D) γ lobulin 370. Total iron content of the normal adult is (A) 1 2 (D) 7 10 m
m (C) 4 5
m (B) 3 4
m
371. Absorption of phosphorous from diet is favoured by (A) (B) (C) (D) Moderate amount of fat Acidic environment Hi h calcium content Hi h phytic acid 372. Daily intake of potassium for a normal person should be (A) 1 B) 2 m (D) 4 m
m (C) 3
m (
373. Absorption of calcium decreases if there is hi h concentration in the diet of (A) Copper (C) Ma nesium (B) Sodium (D) Cadmium 374. Of the followin hi hest concentration of calcium is seen in (A) Blood (C) Muscle (B) CSF (D) Nerve 375. Cobalt is essential component of (A) Vitamin B1 (C) Vitamin B12 (A) (B) (C) (D) (B) Vitamin B6 (D) All of these 376. Iodine is required in human body for Formation of thyroxine Formation of Gl utathione Formation of potassium iodide Adrenalin 377. A hypochromic necrocytic anaemia with increase Fe stores in the bone marrow may be (A) (B) (C) (D) Folic acid responsive Vitamin B12 responsive Pyridoxine responsive Vitamin C responsive
MINERAL METABOLISM 385. In case of wilson’s disease, the features include all of the followin except (A) (B) (C) (D) Pro ressive hepatic cirrhosis Keyser Fleisher rin Aminoaciduri a Urinary excretion of Cu is decreased 203 393. Which of the followin is true? Hypochromic anaemia is not due to iron defi ciency except (A) Serum ‘Fe’ is hi h (B) Normal/low transferrin (C) Stainable iron i n bone marrow (D) Iron therapy is affective 394. Cytosolic superoxide dismutase contains (A) Zn only (C) Zn and Cu (B) Cu only (D) Mn 386. In Vitamin D poisonin (hyper vitaminosis) (A) Both serum and urinary “Ca” (B) The serum Ca is low and urinary calcium hi h (C) The serum “Ca” is increased and uri nary “Ca” is normal (D) Both serum and urinary “Ca” are low 387. The % of ‘K’ in Extracell ar fluid is about (A) 1% (C) 10% (B) 2 to 3% (D) 15% 395. A rise in blood ‘Ca’ may indicate (A) Pa et’s disease (B) Vitamin D deficiency (C ) Cushin ’s disease (D) Hypervitaminosis D 396. The essential trace element which catalyzes the formation of Hb in the body is (A) Mn (C) M 397. (B) Se (D) Cu 388. The Fe containin (D) Urobilino en
pi ments is (A) Haematoidin (C) Hemasiderin (B) Bilirubin
389. All of the followin are true of Wilson’s disease except (A) (B) (C) (D) Low total plasma Cu Elevated urinary copper Arthritis Aminoaciduria Zinc is a constituent of the enzyme: (A) Succinate dehydro enase (B) Carbonic an hydrase (C) Mitochondrial superoxide dismutase (D) Aldolase 390. An increased serum ‘Iron’ and decreased ‘Fe’ bindin capacity are found in (A) (B) (C) (D) Fe deficiency anaemia Sideroblastic anaemia Thalassaemia Anaemia of chro mic disorders 398. The active transport of ‘Ca’ is re ulated by __________ which is synthesized in kidnyes. (A) Cholecalciferol (B) Er osterol (C) 25 OH cholecalciferol (D) 1, 25 di OH Cholecalciferol 399. Ceruloplasmin shows the activity (A) As ferroxidase (C) As li ase (B) As reductase (D) As transferase 391. Iron therapy is ineffective in which of the followin conditions: (A) (B) ( C) (D) Chronic blood loss Inadequate Fe intake Hypochromic anaemia of pre nancy Thalassaemia minor 400. The principal cation of extra cellular fluid: (A) K + (C) H+ (B) Na + (D) C a 2+ 392. In hoemochromatosis, the liver is infiltrated with (A) Copper (C) Man anese (B) Iron (D) Chromium 401. What is the principal cation of intracellular fluid? (A) K + (C) Ca2+ (B) N a + (D) M 2+
204 402. What is the normal level of K+ in the serum ? (A) 137–148 mEq/L (B) 120–160 mEq /L (C) 3.9–5.0 mEq/L (D) 0.3–0.59 mEq/L 403. The eneral functions of minerals are ( A) (B) (C) (D) The structural components of body tissues In the re ulation of bo dy fluids In acid base balance All of these MCQs IN BIOCHEMISTRY (C) It is the stored form of iron (D) Non protein moiety 409. What is ceruloplas min? (A) Plasma protein (C) Both A and B (B) Stored form of copper (D) None of t hese 410. The followin are the functions of copper: (A) (B) (C) (D) Constituent of c ytochromes Catalase Tyrosinase All of these 404. What are the functions of potassium? (A) (B) (C) (D) In muscle contraction Cell membrane function Enzyme action All of these 411. Zn is present as prosthetic roup in this enzyme: (A) (B) (C) (D) Carbonic anhydrase Carboxy peptidase Lactate dehydro enase All of these 405. The daily requirement of calcium is (A) 200 m 0 m
(C) 800 m
(B) 400 m
(D) 60
406. The normal serum inor anic phosphorous level is (A) (B) (C) (D) 1.5–2.5 m /10 0 ml 2.5–4.5 m /100 ml 4.5–6.5 m /100 ml 0.5–1.5 m /100 ml 412. Fluorosis is caused due to (A) (B) (C) (D) Excessive intake of fluorine Low intake of fluorine Discoloration of the teeth due to low intake All of these 413. What is the state of iron in transferrin? (A) Ferrous form (C) Both A and B (B) Ferric form (D) None of these 407. When phosphorous level is lowered ? (A) In hyper thyroidism (B) Cirrosis of liver (C) Leukemia (D) Hypothyroidism 408. Ferritin is (A) Coenzyme (B) One of the component of photophosphorylation 414. Haemo lobin formation needs both (A) Iron and Zinc (B) Iron and Calcium (C) Iron and Copper (D) Iron and Ma nesium
MINERAL METABOLISM 205 ANSWERS 1. A 7. C 13. B 19. D 25. C 31. D 37. B 43. D 49. D 55. D 61. A 67. D 73. C 79. A 85. C 91. C 97. B 103. B 109. A 115. A 121. B 127. A 133. D 139. A 145. D 151. C 157. D 163. A 169. A 175. B 181. D 187. B 193. A 199. A 205. A 211. A 217. A 223. B 229. C 235. A 241. D 247. C 2. C 8. B 14. A 20. A 26. C 32. A 38. C 44. A 50. A 56. B 62. D 68. D 74. D 80. D 86. D 92. B 98. A 104. B 110. D 116. A 122. A 128. C 134. C 140. B 146. D 152. C 158. B 164. C 170. D 176. A 182. B 188. C 194. D 200. C 206. B 212. C 218. A 224. D 230. A 236. C 242. B 248. A 3. C 9. C 15. B 21. A 27. A 33. D 39. A 45. B 51. D 57. B 63. D 69. C 75. D 81. A 87. A 93 . D 99. D 105. A 111. C 117. D 123. A 129. A 135. C 141. A 147. B 153. B 159. A 165. C 171. A 177. A 183. A 189. B 195. D 201. B 207. C 213. B 219. B 225. B 231 . C 237. A 243. A 249. A 4. A 10. D 16. A 22. A 28. C 34. A 40. A 46. A 52. A 58 . A 64. D 70. D 76. B 82. A 88. C 94. C 100. A 106. D 112. D 118. A 124. B 130. A 136. C 142. D 148. D 154. D 160. D 166. A 172. A 178. C 184. D 190. C 196. D 2 02. A 208. D 214. A 220. D 226. A 232. B 238. B 244. A 250. D 5. B 11. C 17. B 2 3. C 29. C 35. B 41. B 47. A 53. A 59. C 65. B 71. A 77. A 83. C 89. D 95. B 101 . C 107. C 113. A 119. D 125. A 131. B 137. A 143. C 149. C 155. A 161. C 167. A 173. A 179. A 185. C 191. C 197. A 203. D 209. B 215. B 221. A 227. C 233. C 23 9. A 245. A 251. D 6. B 12. C 18. D 24. B 30. A 36. D 42. A 48. D 54. A 60. D 66 . D 72. B 78. C 84. A 90. D 96. C 102. A 108. B 114. B 120. D 126. A 132. C 138. B 144. C 150. D 156. C 162. D 168. D 174. A 180. C 186. A 192. D 198. A 204. A 210. C 216. A 222. A 228. A 234. D 240. B 246. A 252. B
206 253. D 259. A 265. D 271. A 277. D 283. B 289. B 295. C 301. A 307. B 313. D 319 . C 325. D 331. B 337. C 343. C 349. B 355. A 361. C 367. A 373. C 379. D 385. D 391. D 397. B 403. D 409. C 254. C 260. B 266. C 272. D 278. D 284. B 290. C 29 6. C 302. C 308. D 314. C 320. D 326. D 332. B 338. D 344. D 350. C 356. C 362. D 368. C 374. C 380. B 386. A 392. B 398. D 404. D 410. D 255. D 261. D 267. D 2 73. D 279. C 285. C 291. A 297. C 303. D 309. A 315. C 321. C 327. C 333. D 339. C 345. D 351. B 357. A 363. B 369. B 375. C 381. B 387. A 393. D 399. A 405. C 411. D 256. C 262. B 268. D 274. A 280. A 286. C 292. D 298. D 304. A 310. C 316 . C 322. C 328. D 334. A 340. C 346. B 352. B 358. D 364. B 370. C 376. A 382. C 388. C 394. C 400. B 406. B 412. A MCQs IN BIOCHEMISTRY 257. C 263. C 269. A 275. C 281. D 287. C 293. D 299. B 305. D 311. C 317. D 323 . B 329. C 335. B 341. C 347. C 353. B 359. D 365. C 371. B 377. C 383. D 389. C 395. D 401. A 407. A 413. B 258. D 264. C 270. C 276. D 282. A 288. C 294. D 30 0. C 306. B 312. C 318. C 324. B 330. C 336. D 342. D 348. D 354. D 360. A 366. D 372. D 378. B 384. B 390. B 396. D 402. C 408. C 414. C
HORMONE METABOLISM 207 CHAPTER 8 ETABOLISM HORMONE METABOLISM 1. Hormones (A) (B) (C) (D) Act as coenzyme Act as enzyme Influence synthesis of enzymes Belon to B complex roup 6. The number of amino acids in human D) 291
rowth hormone is (A) 91 (C) 191 (B) 151 (
2. Hormone that binds to intracellular receptor is (A) (B) (C) (D) Adrenocortico tropic hormone Thyroxine Follicle stimulatin hormone Gluca on 7. Growth hormone causes hyper lycemia. It is a result of (A) Decreased peripher al utilization of lucose (B) Decreased hepatic production via luconeo enesis ( C) Increased lycolysis in muscle (D) Decrersed lipolysis 8. Acrome aly results due to excessive release of (A) Thyroxine (C) Insulin (B) Growth hormone (D) Glu ca on 3. Hormone that bind to cell surface receptor and require the second messen er c amp is (A) (B) (C) (D) Antidiuretic hormone Cholecystokinin Calcitriol Gastrin 9. Growth hormone is released by (A) (B) (C) (D) Somatostatin Growth hormone rel easin hormone Prolactin release inhibitin hormone Luteinizin releasin hormon e 4. A hormone secreted from anterior pituitary is (A) Growth hormone (B) Vasopres sin (C) Oxytocin (D) Epinephrine 5. A hormone secreted from posterior pituitary is (A) (B) (C) (D) Vasopressin Thyrotropic hormone Prolactin Adrenocorticotropic hormone 10. The number of amino acids in prolactin is (A) 134 (C) 172 (B) 146 (D) 199 11. Adrenocorticotropic hormone (ACTH) is a sin le polypeptide containin amino acid (C) 49 amino acid (B) 39 amino acid (D) 52 amino acid
(A) 25
208 12. Biolo ical activity of ACTH requires (A) 10 N terminal amino acid (B) 24 N t erminal amino acid (C) 24 C terminal amino acid (D) 15 C terminal amino acid 13. ACTH stimulates the secretion of (A) Glucocorticoids (C) Thyroxine (B) Epinephr ine (D) Luteinizin hormone MCQs IN BIOCHEMISTRY 20. A specific cortisol bindin protein, transcortin is a (A) Albumin (C) α2-Glo u lin (A) Zon f sicul t (C) Zon reticul ris (B) α1-Glo ulin (D) β-Glo ulin (B) Zon glomerulos (D) Chrom ffin cells 21. Cortisol is synthesized in 14. Excessive secretion of ACTH c uses (A) Cushing’s syndrome (B) Addison’s dise se (C) Myxoedem (D) Thyrotoxicosis 15. In Cushing’s syndrome- tumour ssoci ted dis e se of dren l cortex, there is (A) Decre sed epinephrine production (B) Excess ive cortisol production (C) Excessive epinephrine production (D) Decre sed corts oil production 16. ACTH induces rise in (A) Cyclic AMP (C) C lcium (B) Cyclic GM P (D) M gnesium 22. All m mm li n steroid hormones ) Pyrimidine (D) Pyrrole
re formed from (A) Purine (C) Cholesterol (B
23. A very efficient inhi itor of steroid iosynthesis is (A) (B) (C) (D) Aminog lutethimide Aminoimid zole Aminoimid zolesuccinyl c r ox mine Aminopterin 24. In dren l gl nd the cholesterol is stored (A) Mostly in the free form (B) M ostly in esterified form (C) L rge mount of free form nd less mount of esteri fied form (D) Equ l mounts of free nd esterified form 25. Aldosterone synthesi s occurs in (A) Zon reticul ris (B) Zon f scicul t (C) Zon glomerulos (D) C hrom ffi n cells 26. In the iosynthesis of cortiol, the sequence of enzymes inv olved is (A) Hydroxyl se–dehydrogen se + isomer se – hydroxyl se (B) Dehydrogen se–hyd roxyl se–isomer se (C) Hydroxyl se–ly se–dehydrogen se isomer se (D) Isomer se–ly se–hydro xyl se–dehydrogen se 27. The defect in dren l cortex responsi le for l ck of gluc ocorticoids nd miner lcorticoids is (A) Androstenedione deficiency (B) 17 α -OH p rogesterone deficiency (C) C-21 hydroxyl se deficiency (D) Testosterone deficien cy 17. The circul ting concentr tion of ACTH in pl sm is (A) 0.05 m µ /100 ml (B) 0. 1–2.0 m µ /100 ml (C) 2.5–3.5 m µ /100 ml (D) 3.0–5.0 m µ /100 ml 18. Hyperglycemic effect f glucocorticoids is due to (A) In ctiv tion of protein phosph t se (B) In ctiv tion of fructose 1,6- iphosph t se (C) Stimul tion of synthesis of pyruv te c r oxyl se (D) Stimul tion of synthesis of eltroxykin se 19. The predomin nt glucoc orticoid is (A) Cortisol (B) Aldosterone (C) Dehydroephi ndrosterone (D) Androst enedione
HORMONE METABOLISM 28. 3-β-Hydroxysteroid dehydrogen se nd β ∆5,4 isomer se c t lyse the conversion of t he we k ndrogen DHEA to (A) Androstenedione (B) Testosterone (C) Progesterone ( D) Estrone 29. In the resting st te pl sm concentr tion of cortisol is (A) (B) (C) (D) 0.4–2.0 µg/100 ml 2.0–4.0 µg/100 ml 5.0–15.0 µg/100 ml 18.0–25.0 µg/100 ml 36. C t mine hormones re 209 (A) 3, 4-Dihydroxy deriv tives of phenylethyl mine (B) p-Hydroxy deriv tives of phenyl cet te (C) p-Hydroxy deriv tives of phenylpyruv te (D) p-Hydroxy deriv ti ves of phenyll ct te 37. The sequenti l steps in the conversion of tyrosine to e pinephrine re (A) Ring hydroxyl tion-dec r oxyl tion-side ch in hydroxyl tion-N -methyl tion (B) Side ch in hydroxyl tion-dec r oxyl tion-ring hydroxyl tion N-m ethyl tion (C) Dec r oxyl tion-ring hydroxyl tion-side ch in hydroxyl tion-N-met hyl tion (D) N-methyl tion-dec r oxyl tion-ring nd side ch in hydroxyl tion 38. The hormone required for uterine muscle contr ction for child irth is (A) Prog esterone (C) Oxytocin (B) Estrogen (D) V sopressin 30. The most import nt effect of ldosterone is to (A) Incre se the r te of tu u l r re sorption of sodium (B) Decre se the r te of tu ul r re sorption of pot ssium (C) Decre se the re sorption of chloride (D) Decre se the ren l re sorpt ion of sodium 31. One of the potent stimul tors of ldosterone secretion is (A) (B) (C) (D) Incre sed sodium concentr tion Decre sed pot ssium concentr tion Inc re sed pot ssium concentr tion Incre sed ECF volume 39. The num er of 18
mino
cids in the hormone oxytocin is (A) 7 (C) 14 (B) 9 (D)
32. In the rennin- ngiotensin system the prim ry hormone is (A) Angiotensinogen (B) Angiotensin I (C) Angiotensin II (D) Angiotensin III 33. Aldosterone rele se is stimul ted y (A) α2-Glo ulin (C) Angiotensin II (B) Renin (D) Growth hormone 40. V sopressin nd oxytocin circul te un ound to proteins nd h ve very short p l sm h lf lives, on the order of (A) 1–2 minutes (C) 5–8 minutes (B) 2–4 minutes (D) 10–12 minutes 41. Mel nogenesis is stimul ted
y (A) MSH (C) LH (B) FSH (D) HCG
34. In the synthesis of Angiotensin I, rennin cts on Angiotensinogen nd cle ve s the (A) (B) (C) (D) Leucine – leucine t 10 nd 11 position V line – tyrosine t 3 nd 4 position Isoleucine – histidine t 5 nd 6 position Proline – histidine t 7 nd 8 position 42. The num er of mino cids in ntidiuretic hormone is (A) 9 (C) 27 43. ADH (A ) Re sor s w ter from ren l tu ules (B) Excretes w ter from ren l tu ules (C) E xcretes hypotonic urine (D) C uses low specific gr vity of urine (B) 18 (D) 36 35. C techol mine hormones re synthesized in the (A) (B) (C) (D) Chrom ffin cel ls of dren l medull Zon glomerulos of dren l cortex Zon f scicul te of dr en l cortex Zon reticul ris of dren l cortex
210 44. Incre sed re sorption of w ter from the kidney is the m jor consequence of the secretion of the hormone? (A) Cortisol (C) V sopressin (B) Insulin (D) Aldos terone MCQs IN BIOCHEMISTRY 52. In the synthetic p thw y of epinephrine, disulfir m ( nt use) inhi its the enzyme: (A) Tyrosine hydroxyl se (B) Dop mine β-hydroxyl se (C) DOPA dec r oxyl se (D) N-methyl tr nsfer se 53. The iosynthesis of oth C techol mine nd seroton in require (A) Tyrosine hydroxyl se (B) N-methyl tr nsfer se (C) Arom tic mino cid dec r oxyl se (D) Tryptoph n pyrrol se 54. Epinephrine stimul tes glycogeno lysis in (A) Liver (B) Muscle (C) Liver nd muscle (D) Kidney 55. A cup of stron g coffee would e expected to (A) Interfere with the synthesis of prost gl ndins (B) Decre se the effect of gluc gon (C) Enh nce the effect of epinephrine (D) P rovide the vit min nicotinic cid 56. Epinephrine is derived from norepinephrine y (A) Dec r oxyl tion (C) Oxid tion (B) Hydroxyl tion (D) N-methyl tion 45. An incre se in the osmol lity of extr cellul r comp rtment will (A) Inhi it ADH secretion (B) Stimul te ADH secretion (C) C use no ch nge in ADH secretion ( D) Stimul te the volume nd osmoreceptor nd inhi it ADH secretion 46. For C tec hol mine iosynthesis the r te limiting enzyme is (A) DOPA dec r oxyl se (B) DOP AMINE β-hydroxyl se (C) Tyrosine hydroxyl se (D) Phenyl l nine hydroxyl se 47. A h ormone which c nnot cross the lood r in rrier is (A) Epinephrine (C) ACTH (B ) Aldosterone (D) TSH 48. The pl sm level of epinephrine is less th n (A) 0.1 ng/ml (C) 0.4 ng/ml (B) 0.2 ng/ml (D) 0.8 ng/ml 49. Epinephrine is r pidly met olized y (A) Mono mine oxid se (B) De min se (C ) Tr nsmin se (D) Dec r oxyl se 50. Pheochromocytom s re tumours of (A) Adren l cortex (C) P ncre s (B) Adren l medull (D) Bone 57. 5 HIAA test is neg tive if p tient is t king (A) Aspirin (C) Phenothi zone ( B) Colchicine (D) Methotrex te 58. Presence of signific nt mount of 5-HIAA in urine indic tes (A) C rcinoid in liver (B) C rcinoid in ppendix (C) Met st sis of c rcinom of liver (D) Hep to m 59. The norm l serum level of triiodothyronine (T3) is (A) 0.2–0.5 ng/ml (C) 2. 0–4.0 ng/ml (B) 0.7–2.0 ng/ml (D) 5.0–8.0 ng/ml 51. A ch r cteristic of pheochromocytom is elev ted urin ry excretion of (A) (B ) (C) (D) Dop mine Tyrosine Vinylm ndelic cid Phenyl l nine
HORMONE METABOLISM 60. The norm l serum level of thyroxine (T4) is (A) (B) (C) (D) 2.0–4.0 µg/100 ml 5. 5–13.5 µg/100 ml 14.0–20.3 µg/100 ml 20.0–25.0 µg/100 ml 69. TSH stimul tes the synthesis lete (A) Thyroxine (C) Epinephrine 211 (B) Adrenocorticoids (D) Insulin 70. Thyroid hormones re synthesized y the iodin tion of the ycine (C) Al nine (B) Phenyl l nine (D) Tyrosine
mino
cid: (A) Gl
61. Excess secretion of thyroid hormones c uses (A) Hyperthyroidism (C) Cretinis m (B) Myxoedem (D) Cushing syndrome 71. The tyrosine residues per molecule of thyroglo ulin is (A) 85 (C) 115 (B) 95 (D) 135 62. Insufficient free T3 nd T4 results in (A) Gr ve’s dise se (B) Mysoedem (C) C ushing syndrome (D) Gig ntism 63. In prim ry hypothyroidism the useful estim tio n is of (A) T 3 (C) TBG (B) T 4 (D) Auto nti odies 72. The percent ge of in ctive precursors (monoidotyrosine n thyroglo ulin is (A) 30 (C) 50 (B) 40 (D) 70 64. When iodine supplies re sufficient the T3 (A) 1 : 2 (C) 1 : 7 (B) 1 : 4 (D) 1 : 10 73. The num er of
mino
nd diiodotyrosine) i
nd T4 r tio in thyroglo ulin is
cids in p r thormone is (A) 65 (C) 115 (B) 84 (D) 122
65. A su st nce which competes with iodide upt ke mech nism y thyroid gl nd is (A) Thiocyn te (C) Fluoride (B) Iodo cet te (D) Fluoro cet te 74. The sequence of mino cid in which the iologic l v lue of p r thormone is (A) 1–15 (C) 30–50 75. PTH (A) Reduces the ren l cle r nce or excretion of c lcium ( B) Incre ses ren l phosph te cle r nce (C) Incre ses the ren l cle r nce of c lc ium (D) Decre ses the ren l phosph te cle r nce 76. The num er of mino cids in the peptide hormone c lcitonin is (A) 16 (C) 32 77. C lcitonin c uses (A) C lci nuri nd phosph turi (B) Decre se in urin ry c lcium (C) Decre se in urin ry p hosphorous (D) Incre se in lood c lcium level (B) 24 (D) 40 (B) 1–34 (D) 50–84 66. Thyroperoxid se enzyme cont ins (A) Heme (C) Zinc (B) Copper (D) M gnesium 67. Thyroproxid se requires hydrogen peroxide s oxidizing gent. The H2O2 is pr oduced y (A) (B) (C) (D) FADH2 dependent enzyme NADH dependent enzyme NADP depe ndent enzyme NADPH dependent enzyme 68. Thyroid stimul ting hormone is dimer. The α -su units of TSH, LH, FSH re id entic l. Thus the iologic l specificity must therefore e β su unit in which the num er of mino cids is (A) 78 (C) 130 (B) 112 (D) 199
212 78. The ch r cteristic of hyperp r thyroidism is (A) Low serum c lcium (B) High serum phosphorous (C) Low serum c lcium nd high serum phosphorous (D) High seru m c lcium nd low serum phosph te 79. P r thyroid hormone (A) (B) (C) (D) Is rel e sed when serum C ++ is too high In ctiv tes vit min D Is secreted when C ++ is too low Depends on vit min K for dequ te ctivity MCQs IN BIOCHEMISTRY 86. In the B ch in of insulin molecule, the Ntermin l (C) Phenyl l nine (B) Threonine (D) Lysine 87. In the B ch in of insulin molecule, the C-termin l (C) Glut m te (B) Tyrosine (D) V line
mino mino
cid is (A) Proline cid: (A) Threonine
88. In the insulin molecule, the num er of interch in disulphide (C) 3 (B) 2 (D) 4
rides is (A) 1
80. δ-Cells of islet of langerhans of pancreas pro uce (A) (B) (C) (D) Pancreatic polypepti e Pancreatic lipase Somatostatin Steapsin 89. In the insulin molecule, the number of intrachain 1 (C) 3 (B) 2 (D) 4
isulphi e bri ges is (A)
81. β -cells of islet of langerhans of the pancreas secrete (A) (B) (C) (D) Insuli n Glucagon Somatostatin Pancreatic polypeptide
90. Insulin exists in polymeric forms, for polymerization it requires (A) Calciu m (C) Manganese (B) Magnesium (D) Zinc 91. The num er of amino acids in pre-pro insulin is (A) 51 (C) 109 92. Proinsuli n has (A) 74 amino acids (B) 86 amino acids (C) 105 amino acids (D) 109 amino ac ids 93. Daily secretion of insulin in a normal adult man is a out (A) 10 units ( C) 30 units (B) 20 units (D) 50 units (B) 86 (D) 132 82. Target tissue of insulin is (A) (B) (C) (D) Red lls GI tract epithelial cells Liver
lood cells Renal tu ular ce
83. Insulin is a dimmer. The num er of amino acids in the A and B chain respecti vely is (A) 19 and 28 (C) 25 and 35 (B) 21 and 30 (D) 29 and 38 84. In A chain of the insulin molecule the Nterminal amino acid is (A) Glycine ( C) Serine (B) Valine (D) Phenylalanine 94. The insulin content of pancreas is a out (A) 50–70 units (C) 150–180 units (B) 1 00–150 units (D) 200–250 units 85. In the A chain of insulin molecule the Cterminal amino acid is (A) Asparagin e (C) Valine (B) Threonine (D) Tyrosine 95. The half life of insulin is (A) < 3–5 minutes (C) < 15 minutes (B) < 8–10 minute s (D) < 15 minutes
HORMONE METABOLISM 96. Insulin stimulates (A) Hepatic glycogenolysis (B) Hepatic glycogenesis (C) L ipolysis (D) Gluconeogenesis 97. Action of insulin on lipid meta olism is (A) It increases lipolysis and increases triglyceride synthesis (B) It decreases lipol ysis and increases triglyceride synthesis (C) It decreases lipolysis and decreas es triglyceride synthesis (D) It increases synthesis of triglyceride and increas ed ketogenesis 98. Insulin increases the activity of (A) Pyruvate kinase (B) Pho sphorylase (C) Triacylglycerol kinase (D) Fructose 2, 6- isphosphatase 99. Insul in decreases the activity of (A) (B) (C) (D) cAMP dependent protein kinase HMG C oA-reductas Phosphodiesterase Acetyl CoA-car oxylase 103. Deficiency of insulin results in (A) (B) (C) (D) Rapid uptake of sugar Lo lood glucose level Decreas e urine output Presence of glucose in urine 213 104. The primary stimulus for insulin secretion is increased. (A) (B) (C) (D) (A ) (B) (C) (D) Blood level of epinephrine Blood level of glucagon Blood level of glucose Water intake Insulin Glucagon Somatostatin Pancreatic polypeptide 105. The α -cells of p ncre s islets produce 106. The num er of mino C) 31 (B) 29 (D) 39
cids in single ch in polypeptide gluc gons is (A) 21 (
107. The h lf life of gluc gons is (A) ~5 (C) ~10 108. Gluc gon enh nces (A) (B) (C) (D) Hep tic glycogenolysis Muscle glycogenolysis Hep tic glycogenesis Lipog enesis (B) ~7 (D) ~12 100. The hum n insulin gene loc ted on the short 8 (B) 17 (D) 20 101. Norm l serum insulin level v ries l (D) 100–120 µU /ml
rm of chromosome: (A) 11 (C) 1
etween (A) 4–25 µU/ml (C) 70–90 µU/ml (B) 25–50 µU
109. Norm l serum gluc gons level in f sting st te v ries etween (A) 0-–10 pg/ml (B) 20–100 pg/ml (C) 200–300 pg/ml (D) 400–500 pg/ml 110. Gluc gon (A) (B) (C) (D) Inc re ses protein synthesis Inhi its lipolysis in dipocytes Incre ses gluconeogene sis in liver Stimul tes muscle glycogenolysis 102. Following is norm l overnight f st nd cup of l ck coffee, di etic wom n feels slightly n usious nd decides to skip re kf st. However she does t ke her shot of insulin. This m y result in (A) Heightened glycogenolysis (B) Hyp oglycemi (C) Incre sed lipolysis (D) Glycosuri 111. Norm l serum free testosterone in dult men v ries 10–30 ng/dl (B) 6–9 ng/dl (D) 50–100 ng/dl
etween (A) 1–5 ng/dl (C)
214 112. Norm l serum free testosterone in dult women v ries l (C) 10–30 ng/dl (B) 0.3–2 ng/dl (D) 50–100 ng/dl
etween (A) 0.0–0.2 ng/d
MCQs IN BIOCHEMISTRY 121. Serum progesterone level during pregn ncy is (A) < 12 ng/ml (C) < 20 ng/ml (B) > 12 ng/ml (D) >24 ng/ml 113. The prepu ert l tot l serum testosterone is (A) <100 ng/100 ml (B) < 200 ng /100 ml (C) <300 ng/100 ml (D) < 400 ng/100 ml 114. The tot l serum testosterone in dult men is (A) (B) (C) (D) 50–100 ng/100 ml 150–250 ng/100 ml 300–1000 ng/100 ml 1000–3000 ng/100 ml 122. Serum progesterone level during lute l ph se is (A) 0.2–203 ng/ml (C) 6.0–30 ng /ml (B) 3.0–5.0 ng/ml (D) 750 ng/ml 123. Androgens re produced e testis Efferent ductules
y (A) (B) (C) (D) Cells of sertoli Leydig cells Ret
124. The leyding cell ctivity is controlled y (A) (B) (C) (D) Intestiti l cell stimul ting hormone Adernocortex stimul ting hormone Thyroid stimul ting hormon e Mel nocyte stimul ting h rmone 115. The tot l serum testosterone in dult women is (A) (B) (C) (D) 0–5 ng/100 ml 10–15 ng/100 ml 20–80 ng/100 ml 100–200 ng/100 ml 125. Stein-leventh l syndrome is due to overproduction of (A) Estrogens (C) G st ogens (B) Androgens (D) Ethinyl estr diol 116. The serum estr diol level in men is (A) 0–5 pg/ml (C) 24–68 pg/ml (B) 5–10 pg/ml (D) 40–60 pg/ml 117. The serum estr diol level in women during 1–10 d ys of menstru l cycle is (A) 0–10 pg/ml (C) 24–68 pg/ml (B) 12–20 pg/ml (D) 80–100 pg/ml 126. The production of progesterone H (C) ACTH (B) TSH (D) MSH
y corpus luteum cell is stimul ted
y (A) L
118. The serum estr diol level in women during 11–20 d ys of menstru l cycle is (A ) 5–30 pg/ml (B) 50–300 pg/ml (C) 500–900 pg/ml (D) 1000 pg/ml 119. The serum estr dio l level in women during 21–30 d ys of menstru l cycle is (A) 10-20 pg/ml (C) 73-14 9 pg/ml (B) 22-66 pg/ml (D) 1000 pg/ml 127. In the iosynthesis of testosterone the r te limiting step is conversion of (A) (B) (C) (D) Cholesterol to pregnenolone Pregnenolone to progesterone Proges terone to 17 α-hydroxy progesterone 17 α-Hydroxy progesterone to ndrostenedione 128. The enzyme c t lyzing conversion of ndrostenedione to testosterone is ) Oxygen se (C) Isomer se (B) Dehydrogen se (D) Dec r oxyl se
(A
120. The serum progesterone level in follicul r ph se is out (A) (B) (C) (D) 0 .2–1.5 ng/100 ml 2.0–2.5 ng/100 ml 3.5–4.5 ng/100 ml 5.0–6.5 ng/100 ml 129. Conversion of testosterone to estr diol requires the enzyme: (A) Arom t se (C) Ly se (B) Dehydrogen se (D) Isomer se
HORMONE METABOLISM 130. The precursor of testosterone is (A) Aldosterone (C) Estrone (B) Methyl tes tosterone (D) Pregnenolone 215 138. The only correct st tement out hormone receptors is (A) Receptors for pro tein hormones re present in cytosol (B) Receptors for steroid hormones re mem r ne ound (C) Hormone-receptor inding is irreversi le (D) Receptors c n underg o down regul tion nd up regul toin 139. Down regul tion is (A) Incre sed destru ction of hormone (B) Feed ck inhi ition of hormone secretion (C) Decre sed c oncentr tion of hormone in lood (D) Decre se in num er of receptors for hor mone 140. All the following st tements out hormones re true except (A) All of them require specific c rriers in pl sm (B) All of them require specific recep tors in t rget cells (C) Some of them re su ject to feed ck regul tion (D) Som e of them incre se the tr nscription of cert in genes 141. All the following st tements out steroid hormones re true except (A) They re hydropho ic (B) They require c rriers to tr nsport them in circul tion (C) Their receptors re intr cellul r (D) They require cyclic AMP s second messenger 142. Cyclic AMP cts s the second messenger for (A) ADH (C) C lcitonin (B) Gluc gon (D) All of these 131. Urin ry 17 ketosteroids (A) Are not found in women (B) Reflect the tot l pr oduction of ndrogenic su st nces (C) Indic te the tot l production of sex hormo ne (D) Are highly ctive ndrogens 132. The hormone me sured in urine to test pr egn ncy is (A) (B) (C) (D) Anterior pituit ry luteinizing hormone Androgen Proge sterone Choroinic gon dotropin 133. Tot l num er of 45 (B) 92 (D) 237
mino
cids in hum n chorionic gon dotropin is (A) 53 (C) 1
134. A hormone produced y corpus luteum nd pl cent , concerned with rel x tion of pelvis tissue is (A) (B) (C) (D) HCG Chorionic som tommotropin Rel xin Proge stins 135. Synthetic progesterone used in or l contr ceptive is (A) Norethindrone (B) Pregnenolone (C) Androstenodione (D) Stil estrol 136. Young women re protected g inst myoc rdi l inf r c tion ec use of the ctivity of (A) Estrogen (B) Prog esterone (C) Growth hormone (D) Oxytocin 137. Hormone receptors possess ll the following properties except (A) All of them re proteins (B) They possess reco gnition dom in (C) They ind hormones with high degree of specificity (D) Num er of receptors in t rget cell is const nt 143. Cyclic AMP cts s the second messenger for Oxytocin (C) ACTH (B) TSH (D) FSH
ll of the following except (A)
216 144. Cyclic GMP cts s the second messenger for (A) (B) (C) (D) Nerve growth f ctor Atri l n triuretic f ctor Epinephrine Norepinephrine MCQs IN BIOCHEMISTRY 153. Tyrosine kin se ctivity is present in (A) (B) (C) (D) α-Adrenergic receptors β-Adrenergic receptors Cholinergic receptors Insulin receptors (B) Dimer (D) Tetr mer 154. Insulin receptor is
(A) Monomer (C) Trimer (A) (B) (C) (D)
145. Some hormones produce their intr cellul r effects y ctiv ting (A) Phospho lip e A1 (B) Phospholip se B (C) Phospholip se C (D) All of these 146. Inositol triphosph te is the second messenger for (A) G strin (C) Oxytocin 147. G-protein s ct s (A) (B) (C) (D) Hormone c rriers Hormone receptors Second messengers Si gn l tr nsducers (B) Cholecystokinin (D) All of these 155. Tyrosine kin se ctivity is present in Acetylcholine receptor PDGF receptor ADH receptor All of these (B) Cyclic GMP (D) Inositol triphosph te 156. Protein kin se C is (C) (D)
ctiv ted
y (A) Cyclic AMP (C) Di cyl glycerol (A) (B)
157. Mel tonin is synthesised in Hypoth l mus Posterior pituit ry gl nd Pine l g l nd Mel nocytes (B) Tyrosine (D) None of these 148. Sign l tr nsducer for gluc gons is (A) (B) (C) (D) Cyclic nucleotide Phos phoinositide Stimul tory G-protein Inhi itory G-protein 158. Mel tonin is synthesised from (A) Phenyl l nine (C) Tryptoph n 149. G-proteins
re (A) Monomers (C) Trimers (B) Dimers (D) Tetr mers
159. Mel nocyte stimul ting hormone is secreted y (A) (B) (C) (D) Pine l gl nd Anterior lo e of pituit ry gl nd Posterior lo e of pituit ry gl nd Intermedi te lo e of pituit ry gl nd 150. G-proteins h ve DP/GTP (D) UDP/UTP
nucleotide
inding site for (A) ADP/ATP (C) CDP/CTP (B) G
160. MSH c uses (A) Dispers l of mel nin gr nules in mel nocytes (B) Incre se in mel nin concentr tion in mel nocytes (C) Decere se in mel nin concentr tion in mel nocytes (D) Incre se in num er of mel nocytes 161. Secretion of MSH is regul ted y (A) (B) (C) (D) Feed ck mech nism Mel tonin Hypoth l mic hormones ACTH 151. The nucleotide inding site of G-proteins is present on their (A) α-Su unit ( C) γ Subunit (B) β-Su unit α- nd β(D) δ-Subunit by (A) GDP-bearing α-Su unit of G-protein (B) GTP- e ring α-Su unit of G-protein (C) GDP- e ring γ Subunit of G protein (D) GTP be arin γ Subunit of G protein 152. A enylate cyclase is activate
HORMONE METABOLISM 162. A hormone synthesised in the hypothalamus is (A) (B) (C) (D) Melatonin Mela nocyte stimulatin hormone Vasopressin Prolactin 217 171. Secretion of somatotrophin is promoted by (A) (B) (C) (D) Somatomedin C Som atostatin Growth hormone releasin hormone Hypo lycaemia 163. Posterior pituitary land secretes (A) (B) (C) (D) Catecholamines Oxytocin Follicle stimulatin hormone Serotonin 172. Human rowth hormone has (A) One polypeptide chain and one intra chain disu lphide bond (B) One polypeptide chain and two intra chain disulphide bond (C) Tw o polypeptide chains joined by one disulphide bond (D) Two polypeptide chains jo ined by two disulphide bond 173. Number of amino acid residues in human rowth h ormone is (A) 51 (C) 191 (B) 84 (D) 198 164. A nonapeptide amon the followin is (A) (B) (C) (D) Antidiuretic hormone I nsulin ACTH Thyrotropin releasin hormone 165. Diabetes insipidus is caused by deficient secretion of (A) Insulin (C) Vaso pressin (B) Gluca on (D) Oxytocin 174. Number of amino acid residues in prolactin is (A) 51 (C) 191 (A) (B) (C) (D ) (B) 84 (D) 198 166. Peripheral vasoconstriction is caused by hi h concentrations of (A) (B) (C) (D) Antidiuretic hormone Melatonin Gluca on Oxytocin 175. Secretion of prolactin is re ulated by Feedback inhibition Prolactin releas in hormone Prolactin release inhibitin hormone All of these (B) Pre nenolone ( D) Pro opiomelanocortin 167. Somatotropin is secreted by (A) Hypothalamus (B) Anterior pituitary (C) Pos terior pituitary (D) Thyroid land 168. Secretion of Insulin like Growth Factor I is promoted by (A) Insulin (B) Gluca on (C) Growth hormone (D) Somatomedin C 1 69. Growth hormone increases (A) Protein synthesis (C) Glyco enolysis (B) Lipo e nesis (D) All of these 176. Precursor of ACTH is (A) Cholesterol (C) Corticotropin 177. All of the followin can be formed from pro opiomelanocortin except (A) α- nd β-MSH (B) β- nd γ Lipotropins (C) α- nd β-Endorphins(D) FSH 178. All the following st tem ents out proopiomel nocortin re true except (A) It is m de up of 285 mino c ids (B) It is synthesised in p rs intermedi nd nterior lo e of pituit ry gl n d (C) It is the precursor of ACTH nd mel tonin (D) It is the precursor of corti cotropin like intermedi te lo e peptide nd endorphins 170. Secretion of growth hormone is inhi ited tin (C) Feed ck inhi ition(D) All of these
y (A) Som tomedin C (B) Som tost
218 179. All the following st tements out ACTH re true except (A) It is tropic hormone (B) Its t rget cells re loc ted in dren l cortex (C) Its receptors re loc ted in the cell mem r ne (D) Its second messenger is inositol triphosph te 180. Regul tion of ACTH secretion occurs through (A) Corticotropin rele sing hor mone (CRH) nd corticotropin rele se inhi iting hormone (CRIH) of hypoth l mus ( B) Feed ck inhi ition y cortisol (C) CRH nd feed ck inhi ition y cortisol ( D) CRIH nd feed ck inhi ition y cortisol 181. ACTH is polypeptide m de up o f (A) 39 mino cids (C) 51 mino cids (B) 41 mino cids (D) 84 mino cids MCQs IN BIOCHEMISTRY 186. All the following st tements out thyrotropin rele sing hormone re true e xcept (A) It is secreted y hypoth l mus (B) It is pent peptide (C) It incre s es the secretion of TSH (D) Its secretion is inhi ited y high level of T3 nd T 4 in lood 187. In m les, luteinising hormone cts on (A) Leydig cells (C) Prost te gl nd (B) Sertoli cells (D) All of these 188. All the following st tements out FSH re true except (A) It is tropic h ormone secreted y nterior pituit ry (B) Its secretion is incre sed y gon dotr opin rele sing hormone (C) It cts on Sertoli cells (D) It incre ses the synthes is of testosterone 189. In m les, secretion of luteinising hormone is inhi ited y (A) Gon dotropin rele sing hormone (B) FSH (C) High lood level of testostero ne (D) Inhi in 190. Secretion of luteinising hormone is incre sed y (A) GnRH (C ) Testosterone (B) FSH (D) None of these 182. CRH is polypeptide m de up of (A) 39 1 mino cids (D) 84 mino cids
mino
cids (C) 51
mino
cids (B) 4
183. Hormon l ctivity of ACTH is completely lost on remov l of (A) 5 C-termin l mino cids (B) 10 C-termin l mino cids (C) 15 C-termin l mino cids (D) Non e of these 184. All the following st tements out TSH re true except (A) It is glycoprotein (B) It is m de up of α- nd -su units (C) Receptor recognition invo lves oth the su units (D) Its su unit is identic l with those of FSH nd LH 185 . All the following st tements out TSH re true except (A) It is tropic horm one (B) It cts on p r -follicul r cells of thyroid gl nds (C) Its receptors re mem r ne- ound (D) Its second messenger is cyclic AMP 191. In structure terone
nd function, HCG resem les (A) FSH (C) GnRH (B) LH (D) Proges
192. Acromeg ly results from overproduction of (A) ACTH during childhood (B) TSH during dult life (C) Growth hormone during childhood (D) Growth hormone during dult life
HORMONE METABOLISM 193. Acromeg ly results in ll the following except (A) Overgrowth of the ones of f ce, h nds nd feet (B) Incre sed st ture (C) Enl rgements of viscer (D) Im p ired glucose toler nce 194. Overproduction of growth hormone during childhood c uses (A) Acromeg ly (B) Gig ntism (C) Cushing’s dise se (D) Simmond’s dise se 195. Decre sed secretion of growth hormone during childhood c uses (A) Simmond’s dise se (B) Cushing’s dise se (C) Dw rfism (D) Cretinism 196. St ture is incre sed in ( A) Gig ntism (B) Acromeg ly (C) Simmond’s dise se(D) Cushing’s dise se 197. An mino cid used for the synthesis of thyroid hormone is (A) Tyrosine (C) Histidine (B ) Tryptoph n (D) Proline 219 202. Thyroid hormones re present in lood (A) In free form (B) In ssoci tion w ith thyroxine inding glo ulin (TBG) (C) In ssoci tion with thyroxine inding p re l umin (TBPA) (D) M inly in ssoci tion with TBG, p rtly in free form nd som etimes in ssoci tion with TBPA lso 203. When thyroxine inding glo ulin nd th yroxine inding pre- l umin re s tur ted with thyroxine, the excess hormone is tr nsported y (A) Al umin (C) Tr nscortin (B) G mm glo ulins (D) None of these 204. Receptors for thyroid hormones re present (A) On the cell mem r ne (B) Acr oss the cell mem r ne (C) Inside the cells (D) In ssoci tion with G-proteins 20 5. Binding of thyroxine to its receptors (A) Activ tes Adenyl te cycl se (B) Act iv tes gu nyl te cycl se (C) Activ tes stimul tory G-protein (D) Incre ses tr nscription 206. The most powerful thyroid hormone is (A) Reverse T3 (C) T 3 (B) DIT (D) T 4 198. An enzyme required for the synthesis of thyroid hormones is (A) Iodin se (B ) Deiodin se (C) Thyroperoxid se (D) Thyroxine synthet se 199. Thyroperoxid se i odin tes (A) (B) (C) (D) Free tyrosine in thyroid gl nd Tyrosine residues of thy roglo ulin Tyrosine residues of thyroxine inding glo ulin Tyrosine residues of thyroxine inding pre l umin 207. The most und nt thyroid hormone in 3 ound to TBG (D) T4 ound to TBG 200. In thyroxine, tyrosine residues 3 nd 5 (B) 2 nd 4 (D) 4 nd 6
lood is (A) Free T3 (C) Free T4 (B) T
re iodin ted
t positions: (A) 1
nd 3 (C)
208. Secretion of thyroid hormones is regul ted y (A) Hypoth l mus (B) Anterior pituit ry (C) Feed ck regul tion (D) All of these 201. Thyroid gl nd t kes up circul ting iodine (A) (B) (C) (D) By simple diffusi on By f cilit ted diffusion By ctive upt ke In exch nge for chloride
220 209. Clinic l fe tures of hyperthyroidism include (A) Goitre, he t intoler nce, weight loss nd t chyc rdi (B) Goitre, tremors, t chyc rdi nd cold intoler nc e (C) Exophth lmos, goiter, t chyc rdi nd loss of ppetite (D) Exophth lmos, g oiter, tremors nd o esity 210. All the following m y occur in hyperthyroidism e xcept (A) Goitre (C) Loss of weight (B) Incre sed ppetite (D) Low BMR MCQs IN BIOCHEMISTRY 217. The second messenger for PTH is (A) Cyclic AMP (C) Di cylglycerol (B) Cycli c GMP (D) Inositol triphosph te 218. PTH c uses ll of the following except (A) Incre sed intestin l sorption of c lcium (B) Incre sed intestin l sorption of phosph te (C) Incre sed tu ul r re sorption of c lcium (D) Incre sed tu ul r re sorption of phosph te 219. S ecretion of PTH is regul ted y (A) Hypoth l mus (B) Anterior pituit ry (C) Feed ck effect of pl sm PTH (D) Feed ck effect of pl sm c lcium 220. A high conc entr tion of PTH in lood c uses (A) Incre se in pl sm c lcium nd inorg nic ph osphorous (B) Decre se in pl sm c lcium nd inorg nic phosphorous (C) Incre se in pl sm c lcium nd decre se in pl sm inorg nic phosphorous (D) Decre se in p l sm c lcium nd incre se in pl sm inorg nic phosphorous 221. Tet ny c n occur (A) In prim ry hyperp r thyroidism (B) In second ry hyperp r thyroidism (C) In idiop thic hypop r thyroidism (D) After ccident l remov l of p r thyroid gl nds 222. Cryst llis tion of insulin occurs in the presence of (A) Chromium (C) Zinc (B) Copper (D) C lcium 211. All the following m y occur in myxoedem except (A) Cold intoler nce (B) Lo w BMR (C) T chyc rdi (D) Dry nd co rse skin 212. Ment l ret rd tion c n occur in (A) (B) (C) (D) Cretinism Juvenile myxoedem Myxoedem Juvenile thyrotoxicosi s 213. P r thyroid hormone (PTH) is synthesised in (A) (B) (C) (D) Chief cells of p r thyroid gl nds Oxyphil cells of p r thyroid gl nds P r follicul r cells of thyroid gl nds Follicul r cells of thyroid gl nd 214. The num er of
mino
cid residues in PTH: (A) 51 (C) 90 (B) 84 (D) 115
215. Amino cid residues which re essenti l for the iologic l ctivity of PTH re (A) (B) (C) (D) N-termin l 34 mino cids N-termin l 50 mino cids C-termin l 34 mino cids C-termin l 50 mino cids 223. D ily secretion of insulin is 40–50 units
out δ – (A) 10–20 mg (C) 10–20 units (B) 40–50 mg (D)
216. Half-life of PTH is (A) A few secon s (C) A few hours (B) A few minutes (D) A few ays 224. Insulin receptors are ecrease m (B) Starvation (D) Kwashiorkor
in number in (A) Obesity (C) Hyperinsulinis
HORMONE METABOLISM 225. Insulin bin ing sites are present on the (A) (B) (C) (D) α-su units of insuli n receptor β-su units of insulin receptor γ subunits of insulin receptor α- nd β−subunits of insu in receptor 234. Insu in increases
221 (A) Protein synthesis (B) Fatty acid synthesis (C) G ycogen synthesis (D) A of these 235. Insu in decreases the synthesis of (A) Hexo inase (B) G uco inase (C ) PEP carboxy inase (D) G ycogen synthetase 236. Diabetes me itus can occur due to a of the fo owing except (A) (B) (C) (D) Deficient insu in secretion Tumo ur of β−ce s Decrease in number of insu in receptors Formation of insu in antibodie s 226. α-Su units of insulin receptor re present (A) (B) (C) (D) Outside the cell m em r ne In the cell mem r ne Across the cell mem r ne In the cytosol 227. β-Su units of insulin receptor re present (A) (B) (C) (D) Outside the cell m em r ne In the cell mem r ne Across the cell mem r ne In the cytosol 237. Hypoglyc emic com c n occur (A) (B) (C) (D) In untre ted di etes mellitus In st rv tion After overdose of or l hypoglyc emic drugs After overdose of insu lin 228. In the insulin receptor, tyrosine kin se dom in is present in (A) α-Su units (C) γ Subunits (B) β-Su units (D) δ-Subunits 238. Secon messenger for glucagons is (A) Cyclic AMP (C) Cyclic GMP (B) Diacylg lycerol (D) Inositol triphosphate 229. Bin ing of insulin to its receptor activates (A) A enylate cyclase (B) Guan ylate cyclase (C) Phospholipase C (D) Tyrosine kinase 230. Insulin receptor is m a e up of (A) (B) (C) (D) One α- nd one β-su unit Two α- nd two β-su unit Two, α two β- nd wo γ subunit One α, one β-one γ and one δ-subunit 239. Number of amino aci
resi ues in glucagons is (A) 29 (C) 51 (B) 34 (D) 84
240. Glucagon secretion increases (A) (B) (C) (D) After a carbohy rate-rich meal After a fat-rich meal When bloo glucose is high When bloo glucose is low 231. Insulin is require for the active uptake of glucose by most of the cells e xcept (A) Muscle cells (C) A ipocytes 232. Insulin ecreases (A) (B) (C) (D) Gly cogenesis Glyolysis Gluconeogenesis Tubular reabsorption of glucose (B) Gluconeo genesis (D) Bloo glucose (B) Renal tubular cells (D) Liver cells 241. The maineffecting of glucagons is to increase (A) (B) (C) (D) Glycolysis in muscles Glycogenolysis in muscles Glycogenolysis in liver Glycogenesis in liver 233. Insulin increases (A) Glycogenesis (C) Lipolysis 242. Tyrosine is require for the synthesis of all of the following except (A) M elatonin (C) Norepinephrine (B) Epinephrine (D) Thyroxine
222 243. Dopamine is synthesise from (A) Dihy roxyphenylalanine (B) Epinephrine (C) Norepinephrine (D) Metanephrine 244. Bloo brain barrier can be crosse by (A) Epinephrine (C) Dopa (B) Dopamine (D) All of these MCQs IN BIOCHEMISTRY 251. Bin ing of catecholamines to α 2 − adrenergic receptors (A) Increases the intra ce u ar concentration cAMP (B) Increases the intrace u ar concentration cGMP ( C) Decreases the intrace u ar concentration cAMP (D) Decreases the intrace u a r concentration cGMP of of of of
245. Epinephrine is synthesised in (A) Chromaffin ce s of adrena medu a (B) S ympathetic gang ia (C) Brain (D) A of these 246. Immediate precursor of epinep hrine is (A) Metanephrine (C) Dopa (B) Norepinephrine (D) Dopamine 252. Phosphoinositide cascade is activated on binding of catecho amines to (A) ( B) (C) (D) α1-Adrenergic receptors α2-Adrenergic receptors β1-Adrenergic receptors β2-Ad renergic receptors 253. Epinephrine decre ses (A) Glycogenesis (B) Glycogenolysis (C) Gluconeogenes is (D) Lipolysis 254. Epinephrine incre ses the concentr tion of free f tty cid s in pl sm y incre sing (A) (B) (C) (D) Extr mitochondri l f tty cid synthesi s Mitochondri l f tty cid ch in elong tion Microsom l f tty cid ch in elong ti on Lipolysis in dipose tissue 247. The chief met olite of c techol mines is (A) Met nephrine (B) Normet nephr ine (C) 3, 4-Dihydroxym ndelic cid (D) V nillylm ndelic cid 248. An enzyme inv olved in c t olism of c techol mines is (A) Dop dec r oxyl se (B) Arom tic mi no cid dec r oxyl se (C) Mono mine oxid se (D) C techol oxid s 249. Norepinephr ine inds m inly to (A) α-Adrenergic receptors (B) β-Adrenergic receptrors (C) Musc rinic receptors (D) Nicotinic receptors 250. Astimul tory G-protein tr nsduces t he sign ls from (A) α1- nd β1- drenergic receptors (B) α2- nd β2- drenergic receptors (C ) α1- nd α2- drenergic receptors (D) β1- nd 2- drenergic receptors 255. Epinephrine incre ses ll of the following except (A) (B) (C) (D) Glycogeno lysis in muscles Lipolysis in dipose tissue Gluconeogenesis in muscles Gluc gon secretion 256. Secretion of c techol mines is incre sed in (A) (B) (C) (D) Cushing’s syndrom e Addison’s dise se Ph eochromocytom Simmond’s dise se 257. Zon glomerulos of dren l cortex synthesises (A) (B) (C) (D) Glucocortico ids Miner locorticoids Androgens Estrogen nd progesterone
HORMONE METABOLISM 258. Cortisol is (A) Glucocorticoid (C) Androgen (A) Hydrocortisone (C) Ald ct one A (B) Miner locorticoid (D) Estrogen (B) Aldosterone (D) Androstenedione 223 267. The second messenger for glucocorticoids is (A) Cyclic AMP (B) Cyclic GMP ( C) Inositol triphosph te (D) No second messenger is required 268. Glucocorticoid s incre se ll of the following except (A) Gluconeogenesis (B) Lipolysis in extr emities (C) Synthesis of elcos noid (D) Hep tic glycogenesis 269. Glucocorticoi ds incre se the synthesis of ll of the following except (A) Glucokin se (B) Glu cose-6-phosph t se (C) Fructose-1, 6- iphosph t se (D) Pyruv te c r oxyl se 270. Secretion of glucocorticoid is regul ted y ll the following except (A) Hypot h l mus (B) Anterior pituit ry (C) Feed ck control y lood glucose (D) Feed c k control y glucocorticoids 271. Excessive secretion of glucocorticoids r ises lood glucose y (A) Decre sing glycogenesis (B) Incre sing glycogenolysis (C) I ncre sing gluconeogenesis (D) Inhi iting HMP shunt 272. Miner lcorticoids regul te the met olism of ll of the following except (A) Sodium (C) C lcium (B) Pot ssium (D) Chloride 259. The m jor miner lcorticoid is 260. Steroid hormones re synthesised in ll of the following except (A) Testes (B) Ov ries (C) Adren l medull (D) Adren l cortex 261. Steroid hormones re syn thesised from (A) (B) (C) (D) Cholesterol 7-Dehydrocholesterol C lcitriol 7-Hydr oxycholesterol 262. A common intermedi te in the synthesis of ll the steroid hormones is (A) ( B) (C) (D) Pregnenolone 17-Hydroxypregnenolone Corticosterone Progesterone 263. A common intermedi te in the synthesis of cortisol nd ldosterone is (A) P rogesterone (C) Estr diol (B) Testosterone (D) None of these 264. A common intermedi te in the synthesis of estrogens is (A) (B) (C) (D) (A) (B) (C) (D) Cortisol Andostenedione Corticosterone 11-Deoxycorticosterone In ss oci tion with tr nscortin chiefly In ssoci tion with l umin to some extent In free form p rtly All of these 265. Glucocorticoids
re tr nsported in
lood
266. All the following st tements out tr nscortin re true except (A) (B) (C) (D) It is synthesised in liver It tr nsports glucocorticoids It tr nsports ldos terone It tr nsports progesterone 273. Miner locorticoids incre se the tu ul r re sorption of (A) Sodium nd c lc ium (B) Sodium nd pot ssium (C) Sodium nd chloride (D) Pot ssium nd chloride
224 274. Miner locorticoids incre se the tu ul r secretion of (A) Sodium (C) Chlorid e (B) Pot ssium (D) Bic r on te MCQs IN BIOCHEMISTRY 283. Secretion of ndrogens is incre sed ormone
y (A) LH (C) ACTH (B) FSH (D) Growth h
275. Secretion of miner lcorticoids is incre sed ) Angiotensin (D) Hypern tr emi
y (A) ACTH (C) Hypok l emi
(B
284. During l te pregn ncy, the m jor source of progesterone is (A) Adren l cort ex (C) Corpus luteum (B) Pl cent (D) Gr fi n follicles 276. In Addison’s dise se, there is excessive retention of (A) Pot ssium (C) Chlor ide (B) Sodium (D) W ter 285. Progesterone is tr nsported in lood y (A) (B) (C) (D) Tr nscortin Sex hor mone inding glo ulin Al umin Testosterone estrogen inding glo ulin 277. In drenogenit l syndrome due to tot l sence of 21-hydroxyl se in dren l cortex, there is (A) (B) (C) (D) Deficient secretion of glucocorticoids Deficie nt secretion of miner lcorticoids Excessive secretion of ndrogens All of these (B) Hydrocortisone (D) Testosterone 286. The m jor met olite of progesterone is (A) Pregnenolone (C) Estr diol (B) Pregn nediol (D) Norethindrone 287. Secretion of progesterone (A) Is more in first h lf of menstru l cycle th n in second h lf (B) Is more in second h lf of menstru l cycle th n in first h lf (C) Rem ins const nt during menstru l cycle (D) Decre ses during pregn ncy 288. Women ecome suscepti le to osteoporosis fter menop use due to decre sed (A) ( B) (C) (D) Secretion of P r thormone Conversion of vit min D into c lcitriol Sec retion of estrogen Secretion of progesterone 278. Spironol ctone is
n
nt gonist of (A) Cortisol (C) Aldosterone
279. Androgens re synthesised in (A) (B) (C) (D) Leydig cells in testes Sertoli cells in testes Seminiferous tu ules Prost te gl nd 280. Testosterone is tr nsported in lood y (A) (B) (C) (D) Tr nscortin Testost erone inding glo ulin Testosterone estrogen inding glo ulin Al umin 289. A hormone used for detection of pregn ncy is (A) (B) (C) (D) Estrogen Proge sterone Oxytocin Chorionic gon dotropin 281. The met olites of ndrogens re (A) (B) (C) (D) 17-Hydroxysteroids 17-Keto steroids 11-Hydroxysteroids 11-Ketosteroids 290. Pl cent secretes ll of the following except (A) (B) (C) (D) FSH Progester one Estrogen Chorionic gon dotropin 282. An ndrogen which is more powerful th n testosterone is (A) Androstenedione (B) Dihydrotestosterone (C) Androsterone (D) Epi ndrosterone
HORMONE METABOLISM 291. G strin is polypeptide m de up of (A) (B) (C) (D) Five mino cids Seventeen mino cids Twenty mino cids
mino
cids Twelve
225 299. Tyrosine hydroxyl se is inhi ited y (A) C techol mines (C) Phenyl l nine ( B) α−Methy dopa (D) Vani y mande ic acid 300. Urinary excretion of vani y made ic acid is increased in (A) Phaeochromoc ytoma (B) Cushing’s syndrome (C) Carcinoid syndrome (D) A dosteronism 301. Iodide upta e by thyroid g and is decreased by (A) Thicyanate (C) Thiourea (B) Thiourac i (D) Methimazo e 292. Bio ogica activity of gastrin is present in the (A) (B) (C) (D) Four N ter mina amino acids Four C termina amino acids Five N termina amino acids Five C termina amino acids 293. A the fo owing statements about β endorphin are true except µ : (A) (B) (C) (D) It is a polypeptide Its precursor is pro-opio-melanocortin Its receptors are represent in rain Its action is locked y morphine 302. Binding of gro th hormone to its receptor results in phosphorylation of (A) JAK-2 (B) Gro th hormone receptor (C) STATs (D) All of these 303. Binding of gr o th hormone to its receptor results in increased transcription of (A) c-fos gen e (C) p-53 gene (B) c-myc gene (D) None of these 294. All the follo ing statements a out epidermal gro th factor are true except (A) It is a protein (B) It possess quaternary structure (C) Its receptor is made up of a single polypeptide chain (D) Its receptor possesses tyrosine kinase dom ain 295. Met-enkephalin is a (A) Tripeptide (C) Octapeptide (B) Pentapeptide (D) Decapeptide 304. Activation of IRS-1, PI-3 kinase and GRB2 is (C) Prolactin (B) Insulin (D) IGF-2 296. Vasoconstrictor effect of ADH is mediated (B) cGMP (D) Angiotensin II
rought a out
y (A) Glucagon
y (A) cAMP (C) Protein kinase C
305. The protein IRS-1 is phosphorylated y (A) Protein kinase A (B) Protein kin ase C (C) Tyrosine kinase activity of insulin receptor (D) Tyrosine kinase activ ity of IGF-1 receptor 306. Phosphorylated IRS-1 activates GRB-2 hich is (A) G-p rotein receptor inding protein-2 (B) Gro th factor receptor inding protein-2 ( C) Gro th hormone receptor inding protein-2 (D) Glucocorticoid receptor inding protein-2 297. The rate limiting step in catecholamine synthesis is catalysed y (A) (B) ( C) (D) Phenylalanine hydroxylase Tyrosine hydroxylase Dopa decar oxylase Phenyle thanolamine N-methyl transferase 298. Dopa decar oxylase is inhi ited ephrine (D) None of these
y (A) Epinephrine (C) α−Methy dopa (B) Norepin
226 307. STAT proteins are (A) Thermostat proteins of brain (B) G ucostat proteins o f hepatocyte ce membrane (C) Short term activators of trans ation (D) Signa t ransduction and activators of transcription 308. Activated phospho ipase C acts on (A) (B) (C) (D) Phosphatidy inosito 4, 5 biphosphate Inosito 1, 4, 5 triph osphate Protein inase C P 3 inase MCQs IN BIOCHEMISTRY 315. Norma range of tota thyroxine in serum is (A) 0.8–2.4 ng/d ) 0.8–2.4 µ g/d (D) 5–12 µ g/d 316. Norma range of tota .4 ng/d (B) 0.1–0.2 µg/d
(C) 5–12 ng/d
tri iodothyronine in serum is (A) 0.1–0.2 ng/d (D) 0.8–2.4 µg/d
(B
(C) 0.8–2
317. Administration of TSH increases serum T3 and T4 in (A) (B) (C) (D) Hyperthy roidism of pituitary srcin Hyperthyroidism of thyroid srcin Hypothyroidism of pituitary srcin Hypothyroidism of thyroid srcin 309. Phospho ipase C is activated by (A) Gs proteins (C) Gq proteins (B) Gi prot eins (D) G12 proteins 310. Proteog ycans are made up of proteins and (A) G ucosamine (C) Sia ic acid ( B) Mannosamine (D) Mucopo ysaccharides 318. High eve of T3 and T4 and ow TSH in serum indicates (A) (B) (C) (D) Hype rthyroidism of pituitary srcin Hypothyroidism of pituitary srcin Hyperthyroidi sm of thyroid srcin Hypothyroidism of thyroid srcin 311 Sweat ch orides are increased in (A) Cystic fibrosis (B) Pancreatic cancer ( C) Acute pancreatitis (D) None of these 312. A the fo owing statements about cystic fibrosis are correct except (A) It is inherited as an autosoma recessive disease (B) It affects a number of exocrine g ands (C) It causes increased swea ting (D) Sweat ch orides are above 60 mEq/L in this disease 313. Radioactive iod ine upta e by thyroid g and 24 hours of a test dose is (A) (B) (C) (D) 1.5–15% of the test done 15–20% of the test done 20–40% of the test done 50–70% of the test done 319. BMR is increased in (A) Endemic goitre (C) Myxoedema (B) Thyrotoxicosis (D) Cretinism 320. Which one of the fo owing statements correct y describes eu aryotic DNA? ( A) If uses DNA po ymerase with nuc ease activities (B) It is rep icated bidirect iona y at many points (C) It contains no repetitive DNA (D) It is non inear 321 . Which one of the fo owing causes frame shift mutation? (A) (B) (C) (D) Transi tion Transversion De etion Substitution of purine to pyrimidine 314. Radioactive iodine upta e by thyroid g and is increased in (A) Endemic goit re (C) Myxoedema (B) Hyperthyroidism (D) Creatinism 322. The second messenger for many hormones is (A) ATP (C) cGMP (B) cyc ic AMP ( D) UTP
HORMONE METABOLISM 323. The most potent hormone concerned with the retention of sodium in the body is (A) Cortisone (C) Corticosterone (B) A dosterone (D) Cortiso 331. In hyperpa rathyroidism there is (A) Hypoca cemia (C) Hypo a emia 227 (B) Hypophophatemia (D) Hyper a emia 332. Insu in resistance is encountered in (A) Addison’s disease (B) Hypothyroidism (C) Hypopituctarism (D) Acromega y 333. Richest source of prostag andins in a h uman ma e is (A) B ood (C) Semen (B) Urine (D) C.S.F. 324. Aspirin b oc s the synthesis of (A) (B) (C) (D) Prostag andins on y Prostac yc ins on y Thromboxanes on y A of these 325. Retention of sodium in the body eads to a retention of (A) (B) (C) (D) Pot assium Water Potassium and water Neither potassium nor water 334. One of the fo owing is not used as a second messenger by hormones: (A) (B) (C) (D) mRNA cAMP Ca cium ions Myoinisoto 1, 4, 5 triphosphate 326. cAMP is so ca ed because it is formed during (A) (B) (C) (D) TCA cyc e Ure a cyc e Rhodopsin cyc e It has a cyc ic structure 335. This pancreatic hormone increases the b ood sugar su in G ucagon Pancreozymin Pancreatic po ypeptide
eve : (A) (B) (C) (D) In
327. Protein bound iodine is _________ bound to protein. (A) Iodine (C) Thyroxin e (B) Thyroid hormones (D) Tri iodo thyronine 336. Which one of the fo owing statements is fu y correct? (A) Hormones are ne eded in the diet (B) Hormones can be e aborated on y by endocrine g ands (C) A the hormones enter the ce s and perform their function (D) Hormones are substa nce synthesized in the body in sma quantities and contro and regu ate metabo ic events 337. T3 is (A) (B) (C) (D) Thyroxine Triodo thyronine Triodo tyrosine Reverse tri iodo thyronine 328. In hypophysectonized anima s, fasting produces (A) (B) (C) (D) Severe hyper g ycemia Hypog ycemia No change in b ood sugar Mi d hyper g ycemia 329. Ca citomica is antagonist to (A) (B) (C) (D) Serotonin Thyroxine Tri iodo t hyronine Para thyroid hormone 330. There is po yuria without g ycosuria in this disorder (A) Diabetes insipidu s (B) Diabetes mi itus (C) Bronze diabetes (D) Juveni e diabetes 338. Whcih of the fo owing hormone is a peptide of ess than ten amino acids? ( A) Insu in (C) Oxytocin (B) Growth hormone (D) Parathyroid hormone
228 339. Tyrosine of thyrog obu in is acted upon by ________ to give mono and diiodo tyrosines. (A) (B) (C) (D) Potassium Iodide Iodine Iodide I Higher va ency stat e of iodine (I+) MCQs IN BIOCHEMISTRY 347. The b ood sugar raising action of the hormone of suprarena cortex is due t o (A) (B) (C) (D) G yconeogenesis G ycogeno ysis G ucagon i e activity due to i nhibition of g omeru ar fi tration of g ucose The chief nutrient for the brain i s g ucose The chief nutrient for the heart is g ucose The g ucostatic ro e of th e iver is damaged The idneys are damaged 340. Whcih of the fo owing hormone does not activate adeny ate cyc ase? (A) (B) (C) (D) Epinephrine G ucagon Parathyroid hormone Insu in 348. Hyper insu inism can cause coma since (A) (B) (C) (D) 341. Pheochromacytoma is a tumor of (A) (B) (C) (D) adrena Pancreas pituitary
medu
a bone head of
349. Which of the fo owing property of prostag andins has been uti ized by chin icians in hospita for (A) (B) (C) (D) Inducing fever Causing inf ammation Effec ting smooth musc e contraction Disaggregation of spermatozoa 342. Which one of the fo owing statements is incorrect? (A) (B) (C) (D) Insu in increases g ucose phosphory ation Insu in increases g yco ysis Insu in augments HMP shunt Insu in promotes g uconeogenesis 350. A major structura difference between estrogens and androgens is the fact t hat (A) The androgens are usua y C21 steroids (B) The estrogens are usua y dig itonin precipitab e (C) The androgens have an aromatic ring (D) The estrogens ha ve an aromatic ring 351. A oxan can experimenta y induce diabetes me itus due to (A) Stimu ation of α cells of the islets of l ngerh ns (B) Necrosis of the β cel ls of the islets (C) Potenti tion of insulin se ctivity (D) Epinephrine like c tion 352. Which of the following llevi tes sthm ? (A) PGE1 only (C) PGF2 (A) T yrosine (C) T urine (B) PGE1 nd PGE2 (D) PGA 343. Which of one ring in the structure of the following is ens (C) Cholesterol (B) Estrogens (D) Bile cids
rom tic? (A) Androg
344. Which of one of the following is not GUT hormone? (A) Motiline (C) G strin (B) Secretion (D) C lcitonin 345. Which of the following hormones re synthesized s prehormones (A) (B) (C) (D) V sopressin nd oxytocin Growth hormone nd insulin Insulin nd p r thyroid hormone Insulin nd Gluc gon 353. Thyroxine is derived from (B) Tyr nine (D) Trypt ine 346. This hormone h s disulphide group: (A) Gluc gon (C) T 4 (B) Insulin (D) Epi nephrine 354. Adrne l cortic l response is poor in (A) Kw shiorkor (C) F tty liver (B) M r smus (D) Atherosclerosis
HORMONE METABOLISM 355. Protein ound iodine in lood is present to the extent of _______ / dL (A) 3–8 mg (C) 3–8 gm 356. Prost gl ndins re (A) (B) (C) (D) C2 uns tur ted cids C27 s tur ted lcohols C20 s tur ted cids C27 s tur ted lcohols (B) 4–8 mg (D) 4–8 gm 229 364. Which of one of the following is rele sed y hypoth l mus? (A) Som tost tin (B) Som totropic hormone (C) Som to medin C (D) Luteinising hormone 365. Which one of the following is not li er ted y the denohypophysis? (A) Growth hormone (B) TSH (C) ACTH (D) Gon dotropin 366. Which of the following hormone is not un der the control of ACTH? (A) Aldosterone (C) Corticosterone (B) Cortisol (D) Deo xycorticosterone 357. Which of tne of the following scientists h s not worked in the field of pro st gl ndins? (A) Voneuler (C) Andre ro et (B) Sult n K rim (D) Kend l 358. The suffix num er in the n mes of prost gl ndins gives the num er of (A) OH groups (C) Acid groups (B) Dou le onds (D) Keto cids 367. Which of the following org n prefers fructose to glucose (A) Liver (C) P nc re s (B) Testes (D) He rt 359. One of the import nt functions of prost cyclins is (A) (B) (C) (D) Inhi iti on of pl telet ggreg tion Contr ction of uterus Decre se of g stric secretion R elieving osthm 368. Tot l synthesis of cre tine c n ys (D) He rt 369. Thyrotropin rele sing hormone is tide (D) Dec peptide
e done
y (A) Liver (C) P ncre s (B) Kidne
(A) Dipeptide (C) Oct peptide (B) Tripep
360. V sopressin is lso known s (A) (B) (C) (D) Antidi iuretic hormone Som totropic hormone Pitoxin
etogenic hormone Antid
370. Hypth l mo _________ gon d l oxis, fill up the l nk with the suit . (A) Adren l (C) Hypophyse l (B) Thyroid (D) P ncre tic 361. Which of the following is used for inducing l V sopressin (B) Prost cyclins (D) Throm ox nes
le word
our? (A) Prost gl ndins (C)
371. The sequence of mino cids in hum n growth hormone nd the synthesis were done y (A) S nger (C) Ch h Holi (A) ACTH (C) Endorphins (A) Adenine (C) Tyrosin e (B) Kre s (D) Molisch (B) β-tropin (D) All of these (B) Adenosine (D) Tryptoph n 362. Which of the following does not h ve disulphide lin (B) V sopressin (D) Gluc gon
ond? (A) Oxytocin (C) Insu
372. Proopiomel nocortin is the precussor of 363. Which is incorrect ? Epinephrin promotes the glycogenolysis in (A) Muscle ( C) He rt (B) Liver (D) None of these 373. Adren lin is synthesized from
230 374. Corticotropin rele sing hormone controls the direct rele se of (A) (B) (C) (D) Pro-opiomel nocortin α MSH β MSH Endorphins MCQs IN BIOCHEMISTRY 382. Aldosteronism will present the chemic l p thology of (A) Addison’s (C) Gr ve’s (B) Cushing’s (D) H rtnup’s 375. The immedi te p rent of α, β nd γ endorphins is (A) (B) (C) (D) Pro opiomelanoco rtin β-lipotropin ATCH Lipoprotein 383. One of the follo ing does not (D) Haptoglo in
ind T3 and T4: (A) Al umin (C) TBPA (B) TBG
384. Epinephrine causes in muscle: (A) Gluconeogenesis (B) Glycogenesis (C) Glyc olysis 385. Reverse T3 is (A) A synthetic compound given counter the effects of T3 (B) Formed from T4 ut has no hormone function (C) Formed y isomerisation of T3 (D) Formed from T4 and has hormone function 386. This pancreatic hormone pro motes hypogenesis: (A) Insulin (C) Stomato station (B) Glucagon (D) Pancreozymin e (D) Glycogenolysis 376. Prolactin release inhi iting hormone is panine (B) Norepinephrine (D) Acetyl choline
elieved to
e (A) Serotonin (C) Do
377. Whcih one of the follo ing is not a symptom of cushing’s disease? (A) Hypergl ycemia (C) Hirsutism (B) Hypernatremia (D) Hyperkalemia 378. Insulin increases the permea ility of glucose across the plasma mem rane of muscle cells y (A) Acting on adenylate cycle (B) By loosening the integrity of the mem rane (C) Through Ca2+ ions (D) By mem rane cruting the hexose carries o f intracellular organelles and making them fuse ith the plasma mem rane 379. So matostatin is produced y (A) Hypothalamus (B) Pancreas (C) Hypothalamus and pan creas (D) Hypothalamus and Adrenals 380. Insulin like gro th hormones are produc ed y (A) Hypophysis (C) Pancreas (B) Liver (D) Thyroid 387. It is unique that the follo ing single antidia etogenic hormone effectively counter acts the several dia etogenic hormones: (A) Glucagon (C) Insulin (B) Gl ucocorticoids (D) Gro th hormone 388. Which of the follo ing statements is correct? (A) (B) (C) (D) Thyroxine inh i its utilization of glucose Insulin increases utilization of glucose Glucagon p romotes muscle glycogenolysis Insulin inhi its lipogenesis from car ohydrates (B ) Protein (D) Cholesterol 389. Steroid hormones are synthesized from (A) Adenine (C) Vitamin 381. In pheochromocytoma, urine e and Arginine
ill have (A) FILGU (C) 5 HIAA (B) VMA (D) Lysin
390. Hormones act only on specific organs or tissues. These are called (A) Activ e sites (B) Reaction centre (C) Target organ/Tissue(D) Physiological site
HORMONE METABOLISM 391. __________ hormone is a single chain polypeptide having 32 amino acids ith molecular eight of 3,600. (A) Testosteron (C) Calcitonine (B) Thyroxine (D) Va sopressin 231 398. Insulin regulates fatty acid synthesis y (A) Dephosphorylating of acetyl C oA car oxylase (B) Activating phosphorylase (C) Inhi iting malonyl CoA formation (D) Controlling carnitine-Acyl CoA transferase activity 399. Hormonal stimulati on of the formation of the second messenger inositol 1,4,5 triphosphate (IP3) qu ickly leads to the release of hich other intracellular messenger? (A) cAMP (C) Calcinon (B) Prostaglandin (D) Leukotriene 392. Which of the follo ing is noted in cushing’s syndrome, a tumor associated dis ease of the adrenal cortex? (A) Decreased production of epinephrine (B) Excessiv e production of epinephrine (C) Excessive production of vasopressin (D) Excessiv e production of cortisol 393. A cup of strong coffee ould e expected to (A) In terfere ith synthesis of prostaglandins (B) Decrease the effects of Glucagon (C ) Enhance the effects of epinephrine (D) Provide the vitamin nicotinic acid 394. Increased rea sorption of ater from the kidney is the major consequence of hi ch of the follo ing hormones? (A) Cortisol (C) Vasopressin (B) Insulin (D) Aldos terone 400. Hormone receptors that stimulate cAMP production (A) are part of a complex of t o proteins that transform the external signal into internal cAMP production (B) are proteins distinct and separate from those that catalyze the production of cAMP (C) cause release of the catalytic su unit upon inding of the hormone ( D) are not very specific and ind a num er of different hormones 401. All the fo llo ing hormones use cAMP as a second messenger except (A) Estrogen (C) Luteiniz ing (B) FSH (D) Glucagon 395. Lack of Glucocorticoids and mineral corticoids might e consequence of hic h of the follo ing defects in the adrenal cortex? (A) Androstenadione deficiency (B) Estrone deficiency (C) 17 α-OH progesterone deficiency (D) C- α-Hydroxyl se def iciency 396. ADP ri osyl tion is the mode of ction of (A) Choler toxin (B) Ace tyl choline (C) Muscerinic receptors (D) Cyclic AMP 397. Which one of the follow ing hormones is derived most completely from tyrosine? (A) Gluc gon (C) Insulin (B) Thyroxine (D) Prost gl ndins 402. All the following hormones promote hyperglycemi Insulin (B) Norepinephrine (D) Gluc gon
except (A) Epinephrine (C)
403. Gluc gon ctiv tes the enzyme denylcycl se which c uses the incre se of l ood sug r level. Hence this hormone is c lled (A) (B) (C) (D) Hypoglycemic f cto r Hyper glycemic f ctor Antidi uritic f ctor Thyrotropin-rele sing f ctor (B) F t (D) C r ohydr te 404. TSH hormone
iochemic lly is
(A) Protein (C) Glycoprotein
232 405. The second ry sexu l ch r cters in fem les is effected MIS (B) Gluco corticoids (D) None of these
y (A) Estrogens (C)
MCQs IN BIOCHEMISTRY 409. Which of the following hormones is not involved in c r ohydr te met (A) ACTH (C) V sopressin (B) Gluc gon (D) Growth hormone
olism?
406. A hypochromic microcytic n emi which incre ses Fe, store in the one m rr ow m y e (A) Folic cid responsive (B) Vit min B12 responsive (C) Pyridoxine re sponsive (D) Vit min C responsive 407. G stric Secretion is regul ted y the hor mone: (A) Gluc gon (C) Epinephrin (B) G strin (D) ACTH 410. In the process of tr nscription, the flow of genetic inform tion is from (A ) DNA to DNA (C) RNA to protein (B) DNA to protein (D) DNA to RNA 411. Anticodon region is NA (B) t-RNA (D) z-DNA
n import nt p rt of the structure of (A) r-RNA (C) m-R
412. Thyroid function is determined B) K 42 (D) I 131
y the use of isotopes: (A) N
24 (C) C 45 (
408. An essenti l gent for converting glucose to glycogen in liver is (A) L tic cid (C) UTP (B) GTP (D) Pyruvic cid 413. Pernicious n emi is di gnosed CO60 (B) P 32 (D) Fe59
y the r dio
ctive su st nce: (A) Cl36 (C)
HORMONE METABOLISM 233 ANSWERS 1. C 7. A 13. A 19. A 25. C 31. C 37. A 43. A 49. A 55. C 61. A 67. D 73. B 79. C 85. A 91. C 97. B 103. D 109. B 115. C 121. D 127. A 133. D 139. D 145. C 151. A 157. C 163. B 169. A 175. C 181. A 187. A 193. B 199. B 205. D 211. C 217. A 223. D 229. D 235. C 241. C 2. B 8. B 14. A 20. C 26. A 32. C 38. C 44. C 50. B 56. D 62. B 68. B 74. B 80. C 86. C 92. B 98. A 104. C 110. C 116. C 122. C 128. B 134.C 140. A 146. D 152. B 158. C 164. A 170. B 176. D 182. B 188. D 194. B 2 00. C 206. C 212. A 218. D 224. A 230. B 236. B 242. A 3. A 9. B 15. B 21. A 27. C 33. C 39. B 45. B 51. C 57. C 63. D 69. A 75. A 81. A 87. A 93. D 99. A 105. B 111. C 117. C 123. B 129. A 135. A 141. D 147. D 153. D 159. D 165. C 171. C 1 77. D 183. D 189. C 195. C 201. C 207. D 213. A 219. D 225. A 231. D 237. D 243. A 4. A 10. D 16. A 22. C 28. A 34. A 40. B 46. C 52. B 58. C 64. C 70. D 76. C 82. D 88. B 94. D 100. A 106. B 112. B 118. B 124. A 130. D 136. A 142. D 148. C 154. D 160. B 166. A 172. B 178. C 184. D 190. A 196. A 202. D 208. D 214. B 22 0. C 226. A 232. C 238. A 244. C 5. A 11. B 17. B 23. A 29. C 35. A 41. A 47. A 53. B 59. B 65. A 71. C 77. A 83. B 89. A 95. A 101. A 107. A 113. A 119. C 125. B 131. B 137. D 143. A 149. C 155. B 161. C 167. B 173. C 179. D 185. B 191. B 197. A 203. A 209. A 215. A 221. D 227. C 233. A 239. A 245. D 6. C 12. B 18. C 24. B 30. A 36. A 42. A 48. A 54. C 60. B 66. A 72. D 78. D 84. A 90. D 96. B 10 2. B 108. A 114. C 120. A 126. A 132. D 138. D 144. B 150. B 156. C 162. C 168. C 174. D 180. C 186. B 192. D 198. C 204. C 210. D 216. B 222. C 228. B 234. D 2 40. D 246. B
234 247. D 253. A 259. C 265. D 271. C 277. D 283. A 289. D 295. B 301. A 307. D 313 . C 319. B 325. B 331. B 337. B 343. B 349. C 355. A 361. A 367. B 373. C 379. C 385. B 391. C 397. B 403. B 409. C 248. C 254. D 260. A 266. C 272. C 278. C 28 4. B 290. A 296. C 302. D 308. A 314. B 320. C 326. D 332. D 338. C 344. D 350. D 356. A 362. D 368. C 374. A 380. B 386. A 392. D 398. A 404. C 410. D 249. A 2 55. C 261. A 267. D 273. C 279. A 285. A 291. C 297. B 303. A 309. C 315. D 321. C 327. B 333. C 339. D 345. C 351. B 357. D 363. C 369. B 375. B 381. B 387. C 393. C 399. C 405. A 411. B 250. D 256. C 262. A 268. C 274. B 280. C 286. B 292 . B 298. C 304. B 310. D 316. B 322. B 328. B 334. A 340. D 346. B 352. B 358. B 364. A 370. C 376. C 382. B 388. B 394. C 400. B 406. D 412. D MCQs IN BIOCHEMISTRY 251. C 257. A 263. A 269. A 275. B 281. B 287. B 293. D 299. A 305. B 311. A 317 . C 323. B 329. D 335. B 341. A 347. A 353. A 359. A 365. D 371. C 377. D 383. D 389. D 395. D 401. A 407. B 413. C 252. A 258. B 264. B 270. C 276. A 282. B 28 8. C 294. B 300. A 306. B 312. C 318. C 324. D 330. A 336. D 342. D 348. A 354. A 360. A 366. A 372. D 378. D 384. D 390. C 396. A 402. C 408. C
NUCLEIC ACIDS 235 CHAPTER 9 NUCLEIC ACIDS 1. A nucleoside consists of (A) (B) (C) (D) Nitrogenous se Purine or pyrimidin e se + sug r Purine or pyrimidine se + phosphorous Purine + pyrimidine se + sug r + phosphorous 7. The chemic l n me of gu nine is (A) 2,4-Dioxy-5-methylpyrimidine (B) 2-Amino6-oxypurine (C) 2-Oxy-4- minopyrimidine (D) 2, 4-Dioxypyrimidine 8. Nucleotides nd nucleic cids concentr tion re often lso expressed in terms of (A) ng (C) meq (B) mg (D) OD t 260 nm 2. A nucleotide consists of (A) A nitrogenous se like choline (B) Purine + pyr imidine se + sug r + phosphorous (C) Purine or pyrimidine se + sug r (D) Pur ine or pyrimidine se + phosphorous 3. A purine nucleotide is (A) AMP (C) CMP ( A) GMP (C) CMP 5. Adenine is (A) (B) (C) (D) 6-Amino purine 2-Amino-6-oxypurine 2-Oxy-4- minopyrimidine 2, 4-Dioxypyrimidine (B) UMP (D) TMP (B) AMP (D) IMP 9. The pyrimidine nucleotide C) UDPG (B) UTP (D) CMP
cting
s the high energy intermedi te is (A) ATP (
4. A pyrimidine nucleotide is 10. The c r on of the pentose in ester link ge with the phosph te in nucleotid e structure is (A) C1 (C) C4 (A) Uridine (C) Gu nosine (B) C3 (D) C5 (B) Cytidin e (D) Adenosine 11. Ur cil
nd ri ose form
6. 2, 4-Dioxypyrimidine is (A) Thymine (C) Ur cil (B) Cystosine (D) Gu nine 12. The most NAD (D) FAD
und nt free nucleotide in m mm li n cells is (A) ATP (C) GTP (B)
236 13. The me n intr cellul r concentr tion of ATP in m mm li n cell is mM (C) 0.1 mM (B) 2 mM (D) 0.2 mM
out (A) 1
MCQs IN BIOCHEMISTRY 21. The nitrogenous se present in the RNA molecule is (A) Thymine (C) X nthine (B) Ur cil (D) Hypox nthine 14. The nucleic cid se found in mRNA ut not in DNA is (A) Adenine (C) Gu nin e (A) (B) (C) (D) (B) Cytosine (D) Ur cil 22. RNA does not cont in (A) Ur cil (C) Thymine (B) Adenine (D) Ri ose 15. In RNA moleule ‘C ps’ Allow tRNA to e processed Are unique to euk ryotic mRNA O ccur t the 3’ end of tRNA Allow correct tr nsl tion of prok ryotic mRNA 23. The sug r moiety present in RNA is (A) Ri ulose (C) Ri ose 24. In RNA molecu le (A) (B) (C) (D) Gu nine content equ ls cytosine Adenine content equ ls ur cil Adenine content equ ls gu nine Gu nine content does not necess rily equ l its c ytosine content. (B) Ar inose (D) Deoxyri ose 16. In contr st to euk ryotic mRNA, prok ryotic mRNA (A) (B) (C) (D) (A) (B) (C) (D) C n e polycistronic Is synthesized with introns C n only e monocistronic H s poly A t il 0–40 nucleotides 40–80 nucleotides 90–300 nucleotides More th n 320 nucleotides 25. Methyl ted purines nd pyrimidines A (C) tRNA (B) hnRNA (D) rRNA 17. The size of sm ll st
re ch r cteristic lly present in (A) mRN
le RNA r nges from
26. Thymine is present in (A) tRNA (B) Ri osom l RNA (C) M mm li n mRNA(D) Prok ryotic mRNA 27. The pproxim te num er of nucleotides in tRNA molecule is (A) 25 (C) 75 (B) 50 (D) 100 18. The num er of sm ll st le RNAs per cell r nges from (A) (B) (C) (D) 10–50,000 50,000–1,00,000 1,00,000–10,00,000 More th n 10 l khs 19. Molecul r weight of heterogenous nucle r RNA (hnRNA) is (A) More th n 10 (C) 104 to 105 7 28. In every cell, the num er of tRNA molecules is (D) 40
t le st (A) 10 (C) 30 (B) 20
(B) 10 to 10 (D) Less th n 104 5 6 29. The structure of tRNA (D) Coil 20. ent s ded
ppe rs like
(A) Helix (C) Clover le f (B) H ir pin
In RNA molecule gu nine content does not necess rily equ l its cytosine cont nor does its denine content necess rily equ l its ur cil content since it i (A) (B) (C) (D) Single str nd molecule Dou le str nded molecule Dou le str n helic l molecule Polymer of purine nd pyrimidine ri onucleotides
30. Although e ch specific tRNA differs from the others in its sequence of nucle
otides, ll tRNA molecules cont in se p ired stem th t termin tes in the seq uence CCA t (A) 3′ Termini (B) 5′ Termini (C) Anticodon rm (D) 3′ 5′ -Termini
NUCLEIC ACIDS 31. Tr nsfer RNAs re cl ssified on the sis of the num er of se p irs in (A) Acceptor rm (C) D rm (B) Anticodon rm (D) Extr rm 41. DNA rich in G-C p ir s h ve 237 (A) 1 Hydrogen ond (B) 2 Hydrogen onds (C) 3 Hydrogen onds (D) 4 Hydrogen on ds 42. The f ct th t DNA e rs the genetic inform tion of n org nism implies th t (A) B se composition should e identic l from species to species (B) DNA se composition should ch rge with ge (C) DNA from different tissues in the s me o rg nism should usu lly h ve the s me se composition (D) DNA se composition i s ltered with nutrition l st te of n org nism 43. The width (helic l di meter) of the dou le helix in B-form DNA in nm is (A) 1 (C) 3 (B) 2 (D) 4 32. In tRNA molecule D rm is n med for the presence of the se: (A) Uridine (C ) Dihydrouridine (A) 5 B se p irs (C) 10 B se p irs (B) Pseudouridine (D) Thymid ine (B) 7 B se p irs (D) 20 B se p irs 33. The
cceptor
rm in the tRNA molecule h s
34. In tRNA molecule, the nticodon rm possesses (A) 5 B se p irs (C) 8 B se p irs (B) 7 B se p irs (D) 10 B se p irs 35. The T ψ C arm in the tRNA molecule possesses the sequence (A) (B) (C) (D) T, p seudouridine and C T, uridine and C T, dih drouridine and C T, adenine and C 44. The number of base pair in a single turn of B-form DNA about the axis of the molecule is (A) 4 (C) 10 (B) 8 (D) 12 36. Double helical structure model of the DNA was proposed b (A) (B) (C) (D) Pa uling and Core Peter Mitchell Watson and Crick King and Wooten (B) Adenine (D) Deox ribose (B) Ribose (D) Ribulose 45. The distance spanned b .0 nm (D) 3.4 nm
one turn of Bform DNA is (A) 1.0 nm (C) 3.0 nm (B) 2
37. DNA does not contain (A) Th mine (C) Uracil (A) Deox ribose (C) L xose 46. In a DNA molecule the th mine concentration is 30%, the guanosine concentrat ion will be (A) 10% (C) 30% (B) 20% (D) 40% 38. The sugar moiet
present in DNA is
47. IN a DNA molecule, the guanosine content is 40%, the adenine content will be (A) 10% (C) 30% (B) 20% (D) 40% 39. DNA rich in A-T pairs have (A) 1 H drogen bond (B) 2 H drogen bonds (C) 3 H drogen bonds(D) 4 H drogen bonds 40. In DNA molecule (A) (B) (C) (D) Guanine con tent does not equal c tosine content Adenine content does not equal th mine cont ent Adenine content equals uracil content Guanine content equals c tosine conten t 48. An increased melting temperature of duplex DNA results from a high content o f (A) (B) (C) (D) Adenine + Guanine Th mine + C tosine C tosine + Guanine C tosi ne + Adenine
238 49. A s nthetic nucleotide analogue, 4-h drox p razolop rimidine is used in the treatment of (A) (B) (C) (D) Acute nephritis Gout C stic fibrosis of lung Multip le m eloma MCQs IN BIOCHEMISTRY 56. In purine bios nthesis carbon atoms at 4 and 5 position and N at 7 position are contributed b (A) Gl cine (C) Alanine (B) Glutamine (D) Threonine 50. A s nthetic nucleotide analogue, used in the chemotherap of cancer and vira l infections is (A) (B) (C) (D) Arabinos l c tosine 4-H drox p razolop rimidine 6-Mercaptopurine 6-Thioguanine 57. N10-form l and N5N10-methen l tetrah drofolate contributes purine carbon ato ms at position (A) 4 and 6 (C) 5 and 6 (B) 4 and 5 (D) 2 and 8 58. In purine nucleus nitrogen atom at 1 position is derived from (A) Aspartate (C) Gl cine (B) Glutamate (D) Alanine 51. Histamine is formed from histidine b the enz me histidine decarbox lase in the presence of (A) NAD (C) HS-CoA (B) FMN (D) B6-PO4 59. The ke s formed b
substance in the s nthesis of purine, phosphoribos l p rophosphate i (A) α-D-ri ose 5-phosph te (B) 5-phospho β-D-ri osyl mine (C) D-ri ose (
D) Deoxyri ose 60. In purine iosynthesis ring closure in the molecule formyl gl ycin mide ri osyl-5phosph te requires the cof ctors: (A) ADP (C) FAD (B) NAD (D) ATP nd Mg++ 52. Inf ntile convulsions due to lesser form tion of g mm mino utyric cid fr om glut mic cid is seen in the deficiency of (A) (B) (C) (D) Glut m te-dehydrog en se Pyridoxine Folic cid Thi min 53. Which of the following mino cids produce v soconstrictor on dec r oxyl t ion? (A) Histidine (C) Threonine (B) Tyrosine (D) Arginine 61. Ring closure of formimidoimid zole c r ox mide ri osyl-5-phosph te yields th e first purine nucleotide: (A) AMP (C) XMP (B) IMP (D) GMP 54. The degr d tion of RNA y p ncre tic ri onucle se produces (A) (B) (C) (D) N ucleoside 2-Phosph tes Nucleoside 5′-phosph tes Oligonucleosides Nucleoside 3′-phosp h te nd oligonucleotide 62. The cof ctors required for synthesis of C) GTP, Mg++ (B) ADP (D) GDP
denylosuccin te
re (A) ATP, Mg++ (
55. Intestin l nucleosid ses ct on nucleosides nd produce (A) Purine se only (B) Phosph te only (C) Sug r only (D) Purine or pyrimidine ses nd sug rs 63. Conversion of inosine monophosph te to x nthine monophosph te is c t lysed y (A) IMP dehydrogen se (B) Formyl tr nsfer se (C) X nthine-gu nine phosphori os yl tr nsfer se (D) Adenine phosphori osyl tr nsfer se
NUCLEIC ACIDS 64. Phosphoryl tion of denosine to AMP is c t lysed y (A) (B) (C) (D) Adenosin e kin se Deoxycytidine kin se Adenylosuccin se Adenylosuccin te synthet se 71. P urine iosynthesis is inhi ited y (A) Aminopterin (C) Methotrex te (B) Tetr cyc lin 239 (D) Chlor mphenicol 72. Pyrimidine nd purine nucleoside iosynthesis sh re PRPP (C) Fum r te (B) Glycine (D) Al nine
common precursor: (A)
65. The m jor determin nt of the over ll r te of denovo purine nucleotide iosyn thesis is the concentr tion of (A) (B) (C) (D) 5-phosphori osyl 1-pyrophosph te 5-phospho β-D-ri osyl mine Glycin mide ri osyl-5-phosph te Formylglycin mide ri os yl-5-phosph te 73. Pyrimidine iosynthesis egins with the form tion from glut mine, ATP nd CO 2, of (A) C r moyl sp rt te (B) Orot te (C) C r moyl phosph te (D) Dihydrooro t te 74. The two nitrogen of the pyrimidine ring re contri uted y (A) Ammoni nd glycine (B) Asp r te nd c r moyl phosph te (C) Glut mine nd mmoni (D) A sp rt te nd mmoni 75. A cof ctor in the conversion of dihydroorot te to oroti c cid, c t lysed y the enzyme dihydroorot te dehydrogen se is (A) FAD (C) NAD (B) FMN (D) NADP 66. An enzyme which cts s llosteric regul tor nd sensitive to oth phosph te concentr tion nd to the purine nucleotides is (A) (B) (C) (D) PRPP synthet se PRPP glut myl midotr nsfer se HGPR T se Formyl tr nsfer se 67. PRPP glut myl midotr nsfer se, the first enzyme uniquely committed to purin e synthesis is feed ck inhi ited y (A) AMP (C) XMP (B) IMP (D) CMP 68. Conversion of formylglycin mide ri osyl5-phosph te to formyl-glycin mide ri osyl-5-phosph te is inhi ited y (A) Az serine (B) Di zonorleucine (C) 6-Merc pt opurine (D) Mycophenolic cid 69. In the iosynthesis of purine nucleotides the AMP feed ck regul tes (A) (B) (C) (D) Adenylosuccin se Adenylosuccin te synthe t se IMP dehydrogen se HGPR T se 76. The first true pyrimidine ri onucleotide synthesized is (A) UMP (C) TMP (B) UDP (D) CTP 77. UDP TP
nd UTP
re formed
y phosphoryl tion from (A) AMP (C) ATP (B) ADP (D) G
70. 6-Merc pto purine inhi its the conversion of (A) (B) (C) (D) IMP→ XMP Ri ose 5 phosph te → PRPP PRPP → 5-phospho → β -D-ri osyl mine Glycin mide ri osyl 5-phosph te → f ormylglycin mide ri osyl-5-phosph te 78. Reduction of ri onucleotide diphosph tes (NDPs) to their corresponding deoxy ri onucleotide diphosph tes (dNDPs) involves (A) FMN (C) NAD (B) FAD (D) NADPH
240 79. Conversion of deoxyuridine monophosph te to thymidine monophosph te is c t l ysed y the enzyme: (A) Ri onucleotide reduct se (B) Thymidyl te synthet se (C) CTP synthet se (D) Orotidylic cid dec r oxyl se 80. d-UMP is converted to TMP y (A) Methyl tion (C) Reduction (B) Dec r oxyl tion (D) De min tion MCQs IN BIOCHEMISTRY 86. The enzyme sp rt te tr nsc r moyl se of pyrimidine ed y (A) ATP (C) AMP (B) ADP (D) CTP 87. In hum ns end product of purine c t ) Ure (D) X nthine
olism is (A) Uric
iosynthesis is inhi it cid (C) All ntoin (B
88. In hum ns purine re c t olised to uric cid due to l ck of the enzyme: (A) Ure se (B) Uric se (C) X nthine oxid se (D) Gu n se 89. In m mm ls other th n h igher prim tes uric cid is converted y (A) (B) (C) (D) Oxid tion to ll ntoin Reduction to mmoni Hydrolysis to mmoni Hydrolysis to ll ntoin 81. UTP is converted to CTP D) Reduction
y (A) Methyl tion (C) Amin tion (B) Isomeris tion (
82. Methotrex te locks the synthesis of thymidine monophosph te y inhi iting t he ctivity of the enzyme: (A) Dihydrofol te reduct se (B) Orot te phosphori osy l tr nsfer se (C) Ri onucleotide reduct se (D) Dihydroorot se 83. A su str te fo r enzymes of pyrimidine nucleotide iosynthesis is (A) Allopurinol (B) Tetr cyli n (C) Chlor mphenicol (D) Puromycin 84. An enzyme of pyrimidine nucleotide iosy nthesis sensitive to llosteric regul tion is (A) Asp rt te tr nsc r moyl se (B ) Dihydroorot se (C) Dihydroorot te dehydrogen se (D) Orotidylic cid dec r oxyl se 85 An enzyme of pyrimidine nucleotides iosynthesis regul ted t the genetic level y pp rently coordin te repression nd derepression is (A) C r moyl pho sph te synthet se (B) Dihydroorot te dehydrogen se (C) Thymidine kin se (D) Deox ycytidine kin se 90. The correct sequence of the re ctions of c t olism of denosine to uric ci d is (A) Adenosine→hypox nthine→x nthine→uric cid (B) Adenosine→x nthine→inosine→uric ci (C) Adenosine→inosine→hypox nthine→ x nthine uric cid (D) Adenosine→x nthine→inosine→hypo nthine uric cid 91. Gout is met olic disorder of c t olism of (A) Pyrimidin e (C) Al nine (B) Purine (D) Phenyl l nine 92. Gout is ch r cterized y incre sed pl sm ) Uric cid (D) Cre tinine
levels of (A) Ure
(C) Cre tine (B
93. Lesch-Nyh n syndrome, the sex linked recessive disorder is due to the l ck o f the enzyme: (A) Hypox nthine-gu nine phosphori osyl tr nsferse (B) X nthine ox id se (C) Adenine phosphori osyl tr nsfer se (D) Adenosine de min se
NUCLEIC ACIDS 94. Lesch-Nyh n syndrome, the sex linked, recessive sence of HGPRT se, m y le d to (A) Compulsive self destructive eh viour with elev ted levels of ur te in serum (B) Hypouricemi due to liver d m ge (C) F ilure to thrive nd meg lo l st ic nemi (D) Protein intoler nce nd hep tic enceph lop thy 95. The m jor c t olic product of pyrimidines in hum n is (A) β-Al nine (C) Uric cid (B) Ure (D) G u nine 101. Genetic inform tion flows from (A) DNA to DNA (B) DNA to RNA (C) RNA to cellul r proteins (D) DNA to cellul r proteins 102. Genetic code is (A) Coll ection of codon (B) Collection of mino cids (C) Collection of purine nucleotid e (D) Collection of pyrimidine nucleotide 241 103. Degener cy of genetic code implies th t (A) Codons do not code for specific mino cid (B) Multiple codons must decode the s me mino cids (C) No nticodo n on tRNA molecule (D) Specific codon decodes m ny mino cids 104. Genetic code is (A) Overl pping (C) Not univers l (B) Non-overl pping (D) Am iguous 96. Orotic ciduri type I reflects the deficiency of enzymes: (A) Orot te phosp hori osyl tr nsfer se nd orotidyl te dec r oxyl se (B) Dihydroorot te dehydroge n se (C) Dihydroorot se (D) C r moyl phosph te synthet se 97. Orotic ciduri t ype II reflects the deficiency of the enzyme: (A) (B) (C) (D) Orot te phosphori osyl tr nsfer se Orotidyl te dec r oxyl se Dihydroorot se Dihydroorot te dehydro gen se 105. mRNA is complement ry to the nucleotide sequence of (A) Coding str nd (C) t RNA (B) Ri osom l RNA (D) Templ te str nd 98. An utosom l recessive disorder, x nthinuri is due to deficiency of the enz ymes: (A) (B) (C) (D) Adenosine de min se X nthine oxid se HGPRT se Tr ns min se 106. In DNA replic tion the enzyme required in the first step is (A) DNA directe d polymer se (B) Unwinding proteins (C) DNA polymer se (D) DNA lig se 107. The s m llest unit of DNA c p le of coding for the synthesis of polypeptide is (A) Operon (C) Cistron (B) Repressor gene (D) Replicon 99. Enzymic deficiency in β- minoiso utyric ciduri is (A) (B) (C) (D) Adenosine de min se X nthine oxid se Orotidyl te dec r oxyl se Tr ns min se (B) mRNA (D) t RNA 100. Polysomes l ck in (A) DNA (C) rRNA 108. Termin tion of the synthesis of the RNA molecule is sign led y sequence in the templ te str nd of the DNA molecule, sign l th t is recognized y ter min tion protein, the (A) Rho (ρ) facto (C) δ facto (B) σ facto (D) ε facto
242 109. Aft t mination of th ynth i of RNA mol cul , th co nzym pa a t f om th DNA t mplat . Th co nzym th n cogniz a p omot at which th ynth i of a n w RNA mol cul comm nc , with th a i tanc of (A) Rho (ρ) fa cto (C) β facto (B) δ facto (D) σ facto MCQ IN BIOCHEMISTRY 114. All p i now ox a va iant of th qu nc : (B) 5′–GAGCCA –3′ (A) 5′–TATAAT –3′ ( AA –3′ (D) 5′–TCCTAG –3′ 115. 5’-T minu of mRNA mol cul i capp with (A) (B) (C) (D) o in t ipho phat 7-M thylguano in t iphophat A no in t ipho phat A no in ipho phat 110. In th p oc of t an c iption in act ial c ll (A) Initiation qui ho p ot in (B) RNA polym a inco po at m thylat a in co ct qu nc (C) Both th igma unit an co nzym of RNA polym a a qui fo acc u at p omoto it in ing (D) P ima i n c a y fo initiation 111. Th co ct tat m nt conc ning RNA an DNA polym a i (A) RNA polym a u nucl o i ipho phat (B) RNA polym a qui p im an a a at 5’ n of th g owing polynucl oti chain (C) DNA polym a can a nucl oti at oth n of th chain (D) All RNA an DNA polym a can a nucl oti only at t h 3’ n of th g owing polynucl oti chain 112. Th uka yotic nucl a ch omo o mal DNA (A) I a lin a an un anch mol cul (B) I not a ociat with a p cific m m anou o gan ll (C) I not plicat micon vativ ly (D) I a out of th am iz a ach p oka yotic ch omo 113. Th function of a p o p ot in in an op on y t m i to p v nt ynth i y in ing to (A) Th i o om (B) A p cific gion of th op on p v nting t an c iption of t uctu al g n (C) Th RNA polym a (D) A p cific gion of th mRNA p v nting t an la tion to p ot in 116. Th
fi
t co on to
t an lat
on mRNA i
(A) AUG (C) GGA (B) GGU (D) AAA
117. AUG, th only i ntifi co on fo m thionin i impo tant a (A) (B) (C) ( D) A l a ing facto fo p pti chain A chain t minating co on R cognition it on tRNA A chain initiating co on 118. In io ynth i of p ot in th chain t minating co on a ) UAA, UAG an UGA UGG, UGU an AGU AAU, AAG an GAU GCG, GCA an 119. Th fo mation of initiation compl x u ing p ot in to : (A) IF-III (C) EF-II (B) EF-I (D) IF-I
ynth
i
(A) (B) (C) (D GCU qui
a fac
120. Th amino t minal of all polyp pti chain at th tim of ynth i in E. coli i tagg to th amino aci i u : (A) M thionin (B) S in (C) N-fo myl m thinin (D) N-fo mal in 121. Initiation of p ot in ynth i gin with in ing of (A) (B) (C) (D) 40 S i o omal unit on mRNA 60S i o omal unit Cha gi ng of tRNA with p cific amino aci Attachm nt of aminoacyl tRNA on mRNA
NUCLEIC ACIDS 122. Initiation of p ot in
ynth
i
qui
(A) ATP (C) GDP (B) AMP (D) GTP
243 129. Th nucl ophilic attack on th t ifi ca oxyl g oup of th p pti yl-tR NA occupying th P it an th α - mino group of the new mino cyl tRNA, the num er of ATP required y the mino cid on the ch rged tRNA is (A) Zero (C) Two (B ) One (D) Four
123. The enzyme mino cyl tRNA synthet se is involved in (A) Dissoci tion of di sch rged tRNA from 80S ri osome (B) Ch rging of tRNA with specific mino cids ( C) Termin tion of protein synthesis (D) Nucleophilic tt ck on esterified c r ox yl group of peptidyl tRNA 124. In the process of ctiv tion of mino cids for p rotein synthesis, the num er of high energy phosph te ond equiv lent utilised i s (A) 0 (C) 2 (B) 1 (D) 4 130. Tr nsloc tion of the newly formed peptidyl tRNA t the A site into the empt y P site involves (A) EF-II, GTP (B) EF-I, GTP (C) EF-I, GDP (D) Peptidyl tr nsf er se, GTP 131. In euk ryotic cells (A) Formyl ted tRNA is import nt for initi t ion of tr nsl tion (B) Cyclohex mide locks elong tion during tr nsl tion (C) Cy tosolic ri osomes re sm ller th n those found in prok ryotes (D) Erythromycin i nhi its elong tion during tr nsl tion 132. The mushroom poison m nitin is n in hi itor of (A) Protein synthesis (C) DNA synthesis (B) mRNA synthesis (D) Adenos ine synthesis 125 Tr nsl tion results in rRNA
product known
s (A) Protein (C) mRNA (B) tRNA (D)
126. In the process of elong tion of ch in inding of mino cyl tRNA to the A s ite requires (A) A proper codon recognition (B) GTP (C) EF-II (D) GDP 127. The n ewly entering mino cyl tRNA into A site requires (A) EF-II (C) mRNA (B) Ri oso m l RNA (D) EF-I 133. Tetr cylin prevents synthesis of polypeptide y (A) Blocking mRNA form tion from DNA (B) Rele sing peptides from mRNA-tRNA complex (C) Competing with mRNA for ri osom l inding sites (D) Preventing inding of mino cyl tRNA 134. In pro k ryotes, chlor mphenicol (A) C uses prem ture rele se of the polypeptide ch in (B) C uses misre ding of the mRNA (C) Depolymerises DNA (D) Inhi its peptidyl tr nsfer se ctivity 128. The α- mino group of the new mino cyl tRNA in the A site c rries out nucl eophilic tt ck on the esterified c r oxyl group of the peptidyl tRNA occupying the P site. This re ction is c t lysed y (A) DNA polymer se (B) RNA polymer se (C) Peptidyl tr nsfer se (D) DNA lig se
244 135 Streptomycin prevents synthesis of polypeptide y (A) (B) (C) (D) Inhi iting initi tion process Rele sing prem ture polypeptide Inhi iting peptidyl tr nsfer se ctivity Inhi iting tr nsloc tion MCQs IN BIOCHEMISTRY 144. The enzyme DNA lig se (A) Introduces superhelic l twists (B) Connects the e nd of two DNA ch ins (C) Unwinds the dou le helix (D) Synthesises RNA primers 14 5. Restriction endonucle ses (A) Cut RNA ch ins t specific loc tions (B) Excise introns from hnRNA (C) Remove Ok z ki fr gments (D) Act s defensive enzymes to protect the host cteri l DNA from DNA of foreign org nisms 146. The most like ly leth l mut tion is (A) Su stitution of denine for cytosine (B) Insertion of one nucleotide (C) Deletion of three nucleotides (D) Su stitution of cytosine fo r gu nine 147. In the following p rti l sequence of mRNA, mut tion of the temp l te DNA results in ch nge in codon 91 to UAA. The type of mut tion is 88 89 9 0 CAG 91 UAG 92 93 94 GUC GAC (A) Missene (C) Nonsense GGC UAA CCG 136. Erythromycin cts on ri osomes nd inhi it (A) (B) (C) (D) Form tion of ini ti tion complex Binding of mino cyl tRNA Peptidyl tr nsfer se ctivity Tr nsloc tion 137. The inding of prok ryotic DNA dependent RNA polymer se to promoter sites o f genes is inhi ited y the nti iotic: (A) Puromycin (C) Terr mycin (B) Rif myc in (D) Streptomycin 138. The gene which is tr nscri ed during repression is (A) Structur l (C) Promo ter (B) Regul tor (D) Oper tor 139 The gene of l c operon which h s constitutive expression is (A) i (C) z (B) c (D) p (B) Silent (D) Fr me shit 140. The minimum effective size of n oper tor for l c repressor inding is (A) 5 se p irs (C) 15 se p irs (B) 10 se p irs (D) 17 se p irs 148. Restriction endonucle ses recognize nd cut cert in sequence of (A) Singl e str nded DNA (B) Dou le str nded DNA (C) RNA (D) Protein 149. Positive control of induction is est descri ed s control system in which n operon functions (A) Unless it is switched off y derepressed repressor protein (B) Only fter repressor protein is in ctiv ted y n inducer (C) Only fter n inducer prot ein, which c n e in ctiv ted y corepressor, switches it on (D) Only fter n inducer protein, which is ctiv ted y n inducer, switch it on 141 To commence structur l gene tr nscription the region which should e free on l c oper tion is (A) Promoter site (C) Y gene (B) Oper tor locus (D) A gene 142. In the l c operon concept, (B) Inducer (D) Repressor
protein molecule is (A) Oper tor (C) Promoter
143. The c t olite repression is medi ted y c t olite gene n (CAP) in conjunction with (A) AMP (C) cAMP (B) GMP (D) Cgmp
ctiv tor protei
NUCLEIC ACIDS 150. Interferon (A) Is virus specific (B) Is cteri l product (C) Is synthe tic ntivir l gent (D) Requires expression of cellul r genes 151. Repressor in ds to DNA sequence nd regul te the tr nscription. This sequence is c lled (A) A ttenu tor (C) Anti termin tor (B) Termin tor (D) Oper tor 245 158. Defective enzyme in Hurler’s syndrome is (A) (B) (C) (D) (A) (B) (C) (D) α-L-di uronid se Iduron te sulph t se Arylsulph t se B C- cetyl tr nsfer se S k guchi r e ction Million-N sse re ction Hopkins-Cole re ction G s chrom togr phy 159. Presence of
rginine c n
e detected
y
152. Ok z ki fr gment is rel ted to (A) DNA synthesis (B) Protein synthesis (C) mRNA form tion (D) tRNA form tion 153. The region of DNA known s TATA BOX is th e site for inding of (A) DNA polymer se (B) DNA topoisomer se (C) DNA dependent RNA polymer se (D) Polynucleotide phosphoryl se 154. Reverse tr nscript se is c p le of synthesising (A) RNA → DNA (C) RNA → RNA 155. A tetrovirus is (A) Polio vi rus (C) Herpes virus (B) HIV (D) To cco mos ic virus (B) DNA → RNA (D) DNA → DNA 160. A nitrogenous se th t does not occur in mRNA is (A) Cytosine (C) Ur cil ( B) Thymine (D) All of these 161. In nucleotides, phosph te is tt ched to sug r y (A) S lt ond (C) Ester ond (A) AMP (C) ATP (B) Hydrogen ond (D) Glycosidic ond (B) ADP (D) All of the se 162. Cyclic AMP c n
e formed from
163. A su stituted pyrimidine -Iododeoxyuridine Cytisine r
se of ph rm cologic l v lue is (A) (B) (C) (D) 5 inoside 5-Fluorour cil All of these
156. Peptidyl tr nsfer se ctivity is loc ted in (A) Elong tion f ctor (B) A ch rged tRNA molecule (C) Ri osom l protein (D) A solu le cytosolic protein 157. Ul tr violet light c n d m ge DNA str nd c using (A) Two dj cent purine residue to form cov lently ounded dimer (B) Two dj cent pyrimidine residues to form cov lently onded dimer (C) Disruption of phosphodiester se link ge (D) Disrupti on of non-cov lent link ge 164 The ‘tr nsforming f ctor’ discovered y Avery, McLeod d to e (A) mRNA (C) DNA (B) tRNA (D) None of these 165. In DNA, the complement ry osine (D) Thymine
se of
nd McC rty w s l ter foun
denine is (A) Gu nine (C) Ur cil (B) Cyt
166. In DNA, three hydrogen onds re formed etween (A) (B) (C) (D) Adenine gu nine Adenine nd thymine Gu nine nd cytosine Thymine nd cytosine
nd
246 167. Left h nded dou le helix is present in (A) Z-DNA (C) B-DNA (B) A-DNA (D) No ne of these MCQs IN BIOCHEMISTRY 177. The num er of hydrogen (C) Three (B) Two (D) Four
onds
etween
denine
nd thymine in DNA is (A) One
168. Nucle r DNA is present in com in tion with (A) Histones (C) Both (A) ) (B) Non-histones (D) None of these
nd (B
178. The complement ry se of denine in RNA is (A) Thymine (C) Gu nine (A) (B) (C) (D) (B) Cystosine (D) Ur cil 169. Num er of gu nine NA (D) None of these
nd cytosine residues is equ l in (A) mRNA (C) DNA (B) tR
179. Extr nucle r DNA is present in Ri osomes Endopl smic reticulum Lysosomes Mi tochondri (B) Viruses (D) All of these (B) tRNA (D) hnRNA 170. Alk lis c nnot hydrolyse (A) mRNA (C) rRNA (B) tRNA (D) DNA 180. Mitochondri l DNA is present in (A) B cteri A 171. Codons 172. Amino loop
(C) Euk ryotes (A) DNA (C) rRN
re present in (A) Templ te str nd of DNA (B) mRNA (C) tRNA (D) rRNA cid is tt ched to tRNA t (A) 5’-End (C) Anticodon (B) 3’-End (D) DHU
181. Ri othymidine is present in 182. Ten se p irs re present in one turn of the helix in (A) A-DNA (C) C-DNA (A) (B) (C) (D) (B) B-DNA (D) Z-DNA 173. In prok ryotes, the ri osom l su units re (A) 30 S nd 40 S (C) 30 S nd 5 0 S 174. Ri ozymes re (A) Enzymes present in ri osomes (B) Enzymes which com in e the ri osom l su units (C) Enzymes which dissoci te (D) Enzymes m de up of RNA 175. The sm llest RNA mong the following is (A) rRNA (C) mRNA (B) hnRNA (D) tR NA (B) 40 S nd 50 S (D) 40 S nd 60 S 183. Tr nsfer RNA tr nsfers Inform tion from DNA to ri osomes Inform tion from m RNA to cytosol Amino cids from cytosol to ri osomes Proteins from ri osomes to cytosol 184. Cer mid se is deficient in (A) F ry’s dise se (B) F r er’s dise se (C) Kr dise se (D) T y-S chs dise se 185. Cer mide is present in ll of the following e xcept (A) Pl sm logens (C) Sulph tides (B) Cere rosides (D) Sphingomyelin 186. Nucleotides required for the synthesis of nucleic cids c n e o t ined fro m (A) (B) (C) (D) Diet ry nucleic cids nd nucleotides De novo synthesis S lv g e of pre-existing ses nd nucleosides De novo synthesis nd s lv ge 176. The num er of NA (D) rRNA
denine
nd thymine
ses is equ l in (A) DNA (C) tRNA (B) mR
e’s
NUCLEIC ACIDS 187. De novo synthesis of purine nucleotide occurs in (A) Mitochondri smes (B) Cytosol (D) Ri osomes
(C) Micro
247 196. All of the following enzymes re unique to purine nucleotide synthesis exce pt (A) PRPP synthet se (B) PRPP glut myl mido tr nsfer se (C) Adenylosuccin te synthet se (D) IMP dehydrogen se 197. PRPP synthet se is llosteric lly inhi ite d y (A) AMP (C) GMP (B) ADP (D) All of these 188. The nitrogen toms for de novo synthesis of purine nucleotides re provided y (A) (B) (C) (D) Asp rt te nd glut m te Asp rt te nd glycine Asp rt te, glu t mine nd glycine Asp rt te, glut m te nd glycine 189 For de novo synthesis of purine nucleotides, glycine provides (A) (B) (C) (D ) One nitrogen tom One nitrogen nd one c r on tom Two c r on toms One nitrog en nd two c r on toms 198. An llosteric inhi itor of PRPP glut myl MP (B) ADP (D) All of these
mido tr nsfer se is (A) AMP (C) G
190. For de novo synthesis of purine nucleotides, n 1 (C) Nitrogen 7 (B) Nitrogen 3 (D) Nitrogen 9 199. An llosteric inhi itor of B) ADP (D) GDP
sp rt te provides (A) Nitroge
denylosuccin te synthet se is (A) AMP (C) GMP (
191. In the purine nucleus, c r on 6 is contri uted (B) CO2 (D) Glut mine 200. An GDP
y (A) Glycine (C) Asp rt te
llosteric inhi itor of IMP dehydrogen se is (A) AMP (C) GMP (B) ADP (D)
192. 5-Phosphori osyl-1-pyrophosph te is required for the synthesis of (A) Purin e nucleotides (B) Pyrimidine nucleotides (C) Both (A) nd (B) (D) None of these 193. Inosine monophoph te is n intermedi te during the de novo synthesis of (A) AMP nd GMP (C) CMP nd TMP (B) CMP nd UMP (D) All of these 201. GMP is n llosteric inhi itor of ll the following except (A) PRPP synthet se (B) PRPP glut myl mido synthet se (C) IMP dehydrogen se (D) Adenylosuccin t e synthet se 202. AMP is n llosteric inhi itor of (A) PRPP synthet se (B) Aden ylosucci nte synthet se (C) Both (A) nd (B) (D) None of these 203. The first re ction unique to purine nucleotide synthesis is c t lysed y (A) PRPP synthet se (B) PRPP glut myl mido tr nsfer se (C) Phosphori osyl glycin mide synthet se ( D) Formyl tr nsfer se 194. X nthosine monophosph te is TMP (C) AMP (B) CMP (D) GMP
n intermedi te during de novo synthesis of (A)
195. In the p thw y of de novo synthesis of purine nucleotides, ll the followin g re llosteric enzymes except (A) (B) (C) (D) PRPP glut myl mido tr nsfer se Adenylosuccin te synthet se IMP dehydrogen se Adenylosuccin se
248 204. Free purine ses which c n e s lv ged re (A) Adenine nd gu nine (B) Ade nine nd hypox nthine (C) Gu nine nd hypox nthine (D) Adenine, gu nine nd hypo x nthine 205. The enzyme required for s lv ge of free purine ses is (A) Adenin e phosphori osyl tr nsfer se (B) Hypox nthine gu nine phosphori osyl tr nsfer se (C) Both (A) nd (B) (D) None of these 206. Deoxycytidine kin se c n s lv ge (A ) Adenosine (B) Adenosine nd deoxy denosine (C) Adenosine nd gu nosine (D) Ade nine nd denosine 207. Adenosine kin se c n s lv ge (A) Adenosine (B) Adenosine nd deoxy denosine (C) Adenosine nd gu nosine (D) Adenine nd denosine 208. S lv ge of purine ses is regul ted y (A) Adenosine phosphori osyl tr nsfer se (B) Hypox nthine gu nine phosphori osyl tr nsfer se (C) Av il ility of PRPP (D) None of these 209. The v il le PRPP is used preferenti lly for (A) De novo sy nthesis of purine nucleotides (B) De novo synthesis of pyrimidine nucleotides (C ) S lv ge of purine ses (D) S lv ge of pyrimidine ses 210. The end product o f purine c t olism in m n is (A) Inosine (C) X nthine (B) Hypox nthine (D) Uric cid MCQs IN BIOCHEMISTRY 211. The enzyme common to c t olism of ll the purines is (A) Adenosine de min se (B) Purine nucleoside phosphoryl se (C) Gu n se (D) None of these 212. Uric cid is the end product of purine s well s protein c t olism in (A) M n (C) Bi rds (B) Fish (D) None of these 213. D ily uric (D) 40–600 mg
cid excretion in
dult men is (A) 2–6 mg (C) 150–250 mg (B) 20–40 mg
214. Diet ry purines re c t olised in (A) Liver (B) Kidneys (C) Intesitn l muc os (D) All of these 215. De novo synthesis of pyrimidine nucleotides occurs in (A) Mitochondri (C) Microsomes (B) Cytosol (D) Ri osomes 216. An enzyme common to de novo synthesis of pyrimidine nucleotides nd ure is (A) Ure se (B) C r moyl phosph te synthet se (C) Asp rt te tr nsc r moyl se ( D) Argininosuccin se 217. The nitrogen toms of pyrimidine nucleus re provided y (A) Glut m te (B) Glut m te nd sp rt te (C) Glut mine (D) Glut mine nd sp rt te 218. The c r on toms of pyrimidine nucleus re provided y (A) Glycine nd sp rt te (B) CO2 nd sp rt te (C) CO2 nd glut m te (D) CO2 nd glut mine
NUCLEIC ACIDS 219. Nitrogen t position 1 of pyrimidine nucleus comes from (A) Glut mine (C) G lycine (B) Glut m te (D) Asp rt te 249 228. For the synthesis of TMP from dump, coenzyme is required which is (A) N10 - Formyl tetr hydrofol te (B) N5- Methyl tetr hydrofol te (C) N5, N10- Methylene tetr hydrofol te (D) N5- Formimino tetr hydrofol te 229. All the enzymes requir ed for de novo synthesis of pyrimidine nucleotides re cytosolic except (A) C r moyl phosph te synthet se (B) Asp rt te tr nsc r moyl se (C) Dihydro-orot se ( D) Dihydro-orot te dehydrogen se 230. During de novo synthesis of pyrimidine nuc leotides, the first ring compound to e formed is (A) C r moyl sp rtic cid (B ) Dihydro-orotic cid (C) Orotic cid (D) Orotidine monophosph te 231. Tetr hydr ofol te is required s coenzyme for the synthesis of (A) UMP (C) TMP (B) CMP ( D) All of these 220. Nitrogen t position 3 of pyrimidine nucleus comes from (A) Glut mine (C) G lycine (B) Glut m te (D) Asp rt te 221. The c r on tom t position 2 of pyrimidine nucleus is contri uted O2 (C) Asp rt te (B) Glycine (D) Glut mine
y (A) C
222. Asp rt te contri utes the following c r on toms of the pyrimidine nucelus: (A) C2 nd C4 (C) C2, C4 nd C6 (B) C5 nd C6 (D) C4, C5 nd C6 223. The first pyrimidine nucleotide to s (A) UMP (C) CTP (B) CMP (D) TMP
e formed in de novo synthesis p thw y i
224. Conversion of uridine diphosph te into deoxyuridine diphosph te requires l l the following except (A) (B) (C) (D) Ri onucleotide reduct se Thioredoxin Tetr hydro iopterin NADPH 232. All of the following st tements out thioredoxin reduct se re true except : (A) It requires NADH s coenzyme (B) Its su str tes re ADP, GDP, CDP nd UD P (C) It is ctiv ted y ATP (D) It is inhi ited y dADP 233. De novo synthesis of pyrimidine nucleotides is regul ted y (A) C r moyl phosph te synthet se (B) Asp rt te tr nsc r moyl se (C) Both (A) nd (B) (D) None of these 234. Cytosol ic c r moyl phosph te synthet se is inhi ited y (A) UTP (C) PRPP (B) CTP (D) T MP 225. Amethopterin nd UMP (D) All of these
minopterin decre se the synthesis of (A) TMP (C) CMP (B)
226. For synthesis of CTP nd UTP, the mino group comes from (A) (B) (C) (D) Am ide group of Asp r gine Amide group of glut mine α-Amino group of glut mine α-Amino group of glut m te 227. CTP synthet se forms CTP from (A) (B) (C) (D) CDP DP nd ATP UTP nd glut mine UTP nd glut m te
nd inorg nic phosph te C
250 235. Cytosolic c r moyl phosph te synthet se is ATP (B) PRPP (D) Asp rt te
ctiv ted
y (A) Glut mine (C)
MCQs IN BIOCHEMISTRY 244. All the following st tements out prim ry gout re true except (A) Its inh erit nce is X-linked recessive (B) It c n e due to incre sed ctivity of PRPP s ynthet se (C) It c n e due to incre sed ctivity of hypox nthine gu nine phosph ori osyl tr nsfer se (D) De novo synthesis of purines is incre sed in it 245. Al l of the following st tements out uric cid re true except (A) (B) (C) (D) It is c t olite of purines It is excreted y the kidneys It is undissoci ted t pH ove 5.8 It is less solu le th n sodium ur te 236. Asp rt te tr nsc r P
moyl se is inhi ited
237. The following c nnot e s lv ged in hum n 238. (B) Deoxycytidine (D) Thymidine
y (A) CTP (C) ATP (B) PRPP (D) TM eings: (A) Cytidine (C) Cytosine
β -Aminoiso ytyr te is formed from c t
olism of (A) Cytosine (C) Thymine (B) Ur cil (D) X nthine
246. In inherited deficiency of hypox nthine gu nine phosphori osyl tr nsfer se (A) De novo synthesis of purine nucleotides is decre sed (B) S lv ge of purines is decre sed (C) S lv ge of purines is incre sed (D) Synthesis of uric cid is d ecre sed 247. All of the following st tements out uric cid re true except (A ) It c n e formed from ll ntoin (B) Form tion of uric cid stones in kidneys c n e decre sed y lk linis tion of urine (C) Uric cid egins to dissoci te t pH ove 5.8 (D) It is present in pl sm m inly s monosodium ur te 248. All of the following st tements out prim ry gout re true except (A) (B) (C) (D) Uri c cid stones m y e formed in kidneys Arthritis of sm ll joints occurs commonly Urin ry excretion of uric cid is decre sed It occurs predomin ntly in m les 239. Free mmoni is li er ted during the c t 240. (B) Ur cil (D) All of these
olism of (A) Cytosine (C) Thymine
β -Al nine is formed from c t olism of (A) (B) (C) (D) Thymine Thymine nd cytosine Thymine cil
nd ur cil Cytosine
241. The following coenzyme is required for c t olism of pyrimidine NADH (C) FADH2 (A) (B) (C) (D) (B) NADPH (D) None of these
nd ur
ses: (A)
242. Inherit nce of prim ry gout is Autosom l recessive Autosom l domin nt X-lin ked recessive X-linked domin nt 243. The following norm lity in PRPP synthet se c n c use prim ry gout: (A) (B ) (C) (D) High Vm x Low Km Resist nce to llosteric inih ition. All of these 249. All of the following st tements out llopurinol re true except (A) It is structur l n logue of uric cid (B) It c n prevent uric cid stones in the k idneys (C) It incre ses the urin ry excretion of x nthine nd hypox nthine (D) I t is competitive inhi itor of x nthine oxid se
NUCLEIC ACIDS 250. Orotic ciduri c n e controlled y (A) (B) (C) (D) Or l dministr tion of orotic cid Decre sing the diet ry int ke of orotic cid Decre sing the diet ry int ke of pyrimidines Or l dministr tion of uridine (B) Ri onucleoside monopho sph tes (C) Ri onucleoside diphosph tes (D) Ri onucleoside triphosph tes 251 258. An ltern te su str te for orot te phosphori osyl tr nsfer se is (A) Allopu rinol (C) Hypox nthine (B) X nthine (D) Adenine 251. All of the following occur in orotic ciduri except (A) (B) (C) (D) Incre sed synthesis of pyrimidine nucleotides Incre sed excretion of orotic cid in ur ine Decre sed synthesis of cytidine triphosph te Ret rd tion of growth 259. M mm ls other th n higher prim tes do not suffer from gout ec use they (A) L ck x nthine oxid se (B) L ck denosine de min se (C) L ck purine nucleoside p hosphoryl se (D) Possess uric se 260. Hypouric emi c n occur in (A) X nthine ox id se deficiency (B) Psori sis (C) Leuk emi (D) None of these 261. Synthesis of DNA is lso known s (A) Duplic tion (C) Tr nscription (B) Replic tion (D) Tr n sl tion 252. Inherited deficiency of denosine de min se c uses (A) (B) (C) (D) Hyperuri c emi nd gout Ment l ret rd tion Immunodeficiency Dw rfism 253. Complete sence of hypox nthine gu nine phosph ri osyl tr nsfer se c uses (A) Prim ry gout (C) Uric cid stones (B) Immunodeficiency (D) Lesh-Nyh n syndro me 254. Incre sed urin ry excretion of orotic cid c n occur in deficiency of (A) ( B) (C) (D) Orot te phosphori osyl tr nsfer se OMP dec r oxyl se Mitochondri l or nithine tr nsc r moyl se Any of the ove 262. Replic tion of DNA is (A) Conserv tive (B) Semi-conserv tive (C) Non-conser v tive (D) None of these 263. Direction of DNA synthesis is (A) 5’ → 3’ (C) Both (A) nd (B) (B) 3’ → 5’ (D) None of these 255. All of the following c n occur in LeschNyh n syndrome except (A) (B) (C) (D ) Gouty rthritis Uric cid stones Ret rted growth Self-mutili ting eh viour 264. Form tion of RNA primer: (A) Precedes replic tion (B) Follows replic tion ( C) Precedes tr nscription (D) Follows tr nscription 265. Ok z ki pieces re m de up of (A) RNA (C) RNA nd DNA (B) DNA (D) RNA nd proteins 256. Inherited deficiency of purine nucleoside phosphoryl se c uses (A) Dw rfism (B) Ment l ret rd tion (C) Immunodeficiency (D) Gout 257. Deoxyri onucleotides re formed y reduction of (A) Ri onucleosides 266. Ok z ki pieces A (D) DNA
re formed during the synthesis of (A) mRNA (C) rRNA (B) tRN
252 267. After form tion of replic tion fork (A) Both the new str nds re synthesize d discontinuously (B) One str nd is synthesized continuously nd the other disco ntinuously (C) Both the new str nds re synthesized continuously (D) RNA primer is required only for the synthesis of one new str nd 268. An Ok z ki fr gment co nt ins out (A) (B) (C) (D) 10 Nucleotides 100 Nucleotides 1,000 Nucleotides 10 ,000 Nucleotides MCQs IN BIOCHEMISTRY 275. The unwound str nds of DNA re held p rt y (A) (B) (C) (D) Single str nd inding protein Dou le str nd inding protein Rep protein DNAA protein 276. Deoxyri onucleotides re dded to RNA primer y (A) (B) (C) (D) DNA polymer se I DNA polymer se II DNA polymer se III holoenzyme All of these 269. RNA primer is formed y the enzyme: (A) Ri onucle se (B) Prim se (C) DNA po lymer se I (D) DNA polymer se III 270. In RNA, the complement ry se of denine is (A) Cytosine (C) Thymine (B) Gu nine (D) Ur cil 277. Ri onucleotides of RNA primer re repl ced y deoxyri onucleotides y the e nzyme: (A) (B) (C) (D) DNA polymer se I DNA polymer se II DNA polymer se III hol oenzyme All of these 278. DNA fr gments re se led A gyr se DNA topoisomer se II
y (A) (B) (C) (D) DNA polymer se II DNA lig se DN
271. During replic tion, the templ te DNA is unwound (A) At one of the ends (B) At oth the ends (C) At multiple sites (D) Nowhere 272. During replic tion, unwi nding of dou le helix is initi ted y (A) DNAA protein (C) DNAC protein (A) (B) (C) (D) (B) Dn B protein (D) Rep protein 279. Neg tive supercoils re introduced in DNA ig se DNA gyr se DNA polymer se III holoenzyme
y (A) (B) (C) (D) Helic se DNA l
273. For unwinding of dou le helic l DNA, Energy is provided y ATP Energy is pr ovided y GTP Energy c n e provided y either ATP or GTP No energy is required 280. Reverse tr nscript se ctivity is present in the euk ryotic: (A) (B) (C) (D ) DNA polymer se α DNA polymer se γ Telomerase DNA polymerase II 274. Helicase and DNAB protein cause (A) Rewindin of DNA and require ATP as a s ource of ener y (B) Rewindin of DNA but do not require any source of ener y (C) Unwindin of DNA and require ATP as a source of ener y (D) Unwindin of DNA but do not require any source of ener y 281. DNA polymerase III holoenzyme possesses (A) (B) (C) (D) Polymerase activity 3’→5’ Exonuclease activity 5’→3’ Exonuclease and polymerase activities 3’→5’ Exonuclease merase activities
NUCLEIC ACIDS 282. DNA polymerase I possesses (A) Polymerase activity (B) 3’→5’ Exonuclease activity (C) 5’→3’ Exonuclease activity (D) All of these 283. 3’ → 5’ Exonuclease activity of DNA lymerase I (A) Removes ribonucleotides (B) Adds deoxyribonucleotides (C) Correct s errors in replication (D) Hydrolyses DNA into mononucleotides 284. All of the followin statements about RNA dependent DNA polymerase are true except: (A) It synthesizes DNA usin RNA as a template (B) It is also known as reverse transcri ptase (C) It synthesizes DNA in 5’→3’ direction (D) It is present in all the viruses 2 85. Reverse transcriptase catalyses (A) Synthesis of RNA (B) Breakdown of RNA (C ) Synthesis of DNA (D) Breakdown of DNA 286. DNA A protein can bind only to (A) Positively supercoiled DNA (B) Ne atively supercoiled DNA (C) Both (A) and (B) ( D) None of these 287. DNA topoisomerase I of E. coli catalyses (A) Relaxation of ne atively supercoiled DNA (B) Relaxation of positively supercoiled DNA (C) Con version of ne atively supercoiled DNA into positively supercoiled DNA (D) Conver sion of double helix into supercoiled DNA 288. In mammalian cell cycle, synthesi s of DNA occurs durin (A) S phase (C) Mitotic Phase (B) G1 phase (D) G2 phase 253 289. Meltin temperature of DNA is the temperature at which (A) Solid DNA become s liquid (B) Liquid DNA evaporates (C) DNA chan es from double helix into superc oiled DNA (D) Native double helical DNA is denatured 290. Meltin temperature of DNA is increased by its (A) A and T content (C) Su ar content (B) G and C conte nt (D) Phosphate content 291. Buoynat density of DNA is increased by its (A) A and T content (C) Su ar co ntent (B) G and C content (D) None of these 292. Relative proportions of G and C versus A and T in DNA can be determined by its (A) (B) (C) (D) Meltin temperature Buoyant density Both (A) and (B) None of these 293. Some DNA is present in mitochondria of (A) Prokaryotes (C) Both (A) and (B) (B) Eukaryotes (D) None of these 294. Satellite DNA contains (A) (B) (C) (D) Hi hly repetitive sequences Moderate ly repetitive sequences Non repetitive sequences DNA RNA hybrids 295. Synthesis of RNA and a DNA template is known as (A) Replication (C) Transcr iption (A) 5′ → 3’ (C) Both (A) and (B) (B) Translation (D) Mutation (B) 3′ → 5’ (D) None these 296. Direction of RNA synthesis is 297. DNA dependent RNA polymerase is a (A) Monomer (C) Trimer (B) Dimer (D) Tetr amer
254 298. DNA dependent RNA polymerase requires the followin for its catalytic activ ity: (A) M ++ (C) Both (A) and (B) (B) Mn ++ (D) None of these MCQs IN BIOCHEMISTRY (B) Methylation of some bases (C) Formation of pseudouridine (D) Addition of C C A terminus at 5’ end 307. Post transcriptional modification does not occur in (A) Eukaryotic tRNA (B) Prokaryotic tRNA (C) Eukaryotic hnRNA (D) Prokaryotic mRNA 308. A consensus sequence on DNA, called TATA box, is the site for attachment of (A) (B) (C) (D) RNA dependent DNA polymerase DNA dependent RNA polymerase DNA d ependent DNA polymerase DNA topoisomerase II 299. The initiation site for transcription is reco nized by (A) α−Subunit of DNA dep endent RNA po ymerase (B) β−Subunit of DNA dependent RNA po ymerase (C) Sigma factor (D) Rho factor 300. The termination site for transcription is recognized by (A) α−Subunit of DNA dependent RNA po ymerase (B) β−Subunit of DNA dependent RNA po ymerase (C) Sigma factor (D) Rho factor 301. Mamma ian RNA po ymerase I synthesises (A) mRNA (C) tRNA (A) rRNA (C) tRNA (B) rRNA (D) hnRNA (B) mRNA (D) hnRNA 309. Po yadeny ate tai is not present in mRNA synthesising (A) G obin (C) Apofe rritin (B) Histone (D) Growth hormone 310. Introns are present in DNA of (A) Viruses (C) Man (B) Bacteria (D) A hese
of t
302. Mamma ian RNA po ymerase III synthesises 311. A mamma ian DNA po ymerase among the fo owing is (A) (B) (C) (D) DNA po ym erase α DNA polymer se I DNA polymer se II DNA polymer se IV 303. In m mm ls, synthesis of mRNA is c t lysed y (A) RNA polymer se I (B) RNA polymer se II (C) RNA polymer se III(D) RNA polymer se IV 304. Heterogeneous nuc le r RNA is the precursor of (A) mRNA (C) tRNA (B) rRNA (D) None of these 312. M mm li n DNA polymer se γ is located in (A) Nucleus (C) Mitochondria (B) Nuc leolus (D) Cytosol 305. Post transcriptional modification of hnRNA involves all of the followin ex cept (A) Addition of 7 methyl uanosine triphosphate cap (B) Addition of polyaden ylate tail (C) Insertion of nucleotides (D) Deletion of introns 306. Newly synth esized tRNA under oes posttranscriptional modifications which include all the fo llowin except (A) Reduction in size 313. Replication of nuclear DNA in mammals is catalysed by (A) (B) (C) (D) DNA p olymerase α DNA polymer se β DNA polymer se γ DNA polymerase III 314. Primase activity is present in (A) (B) (C) (D) DNA polymerase II DNA polyme rase α DNA polymer se β DNA polymer se δ
NUCLEIC ACIDS 315. Th mammalian DNA polym a involv in o co a α (B) DNA polymer se β (C) DNA polymer se γ (D) DNA i it th ynth i of (A) DNA (C) tRNA (A) DNA (C) tRNA (C) (D) (A) (B) (C) (D) (B) mRNA (D) RNA (B) mRNA (D)
ction i (A) DNA polym polymerase δ 316. Novo icin inh (A) (B) (C) (D) (A) (B) RNA
255 (D) A th qu nc that int v n tw n two g n 323. All of th following tat m nt a out po t-t an c iptional p oc ing of tRNA a t u xc pt (A) In t on of om tRNA p cu o a mov (B) CCA i a at 3′ n (C) 7-M thylg uano in t ipho phat cap i a at 5′ n (D) Som a a m thylat 324. α-Am nitin inhi its (A) (B) (C) (D) (A) (B) (C) (D) DNA polymer se II of prok ryotes DNA polymer se α of euk ryotes RNA polymer se II of euk ryotes RNA-dependent DNA polymer se DNA in prok ryotes DNA in prok ryotes nd euk ryotes RNA in prok ryot es RNA in prok ryotes nd euk ryotes
317. Ciproflox cin inhi its the synthesis of 318. Ciproflox cin inhi its DNA topisomer se II DNA polymer se I DNA polymer se III DNA gyr se Unwinding of DNA Initi tion of replic tion Initi tion of tr nsl t ion Initi tion of tr nscription Dou le str nded DNA Single str nded DNA Single s tr nded RNA DNA-RNA hy rid 325. Ciproflox cin inhi its the synthesis of 319. Rif mpicin inhi its 326. All of the following st tements out cteri l promoters re true except ( A) They re sm ller th n euk ryotic promoters (B) They h ve two consensus sequen ces upstre m from the tr nscription st r site (C) TATA ox is the site for tt c hment of RNA polymer se (D) TATA ox h s high melting temper ture 327. All of the following st tements out euk ryotic promoters re true except (A) They m y e loc ted upstre m or down stre m from the structur l gene (B) They h ve two c onsensus sequences (C) One consensus sequence inds RNA polymer se (D) Mut tions in promoter region c n decre se the efficiency of tr nscription of the structur l gene 328. In s nger’s method of DNA sequence determin tion, DNA synthesis is st opped y using (A) 1′, 2′- Dideoxyri onucleoside triphosph tes (B) 2′, 3′- Dideoxyri onu cleoside triphosph tes (C) 2′, 4′- Dideoxyri onucleoside triphosph tes (D) 2′, 5′ - Dide oxyri onucleoside triphosph tes 320. Actinomycin D
inds to
321. DNA cont ins some p lindromic sequences which (A) M rk the site for the for m tion of replic tion forks (B) Direct DNA polymer se to turn ck to replic te the other str nd (C) Are recognized y restriction enzymes (D) Are found only in cteri l DNA 322. Introns in genes (A) Encode the mino cids which re remove d during post-tr nsl tion l modific tion (B) Encode sign l sequences which re r emoved efore secretion of the proteins (C) Are the non-coding sequences which re not tr nsl ted
256 329. tRNA genes h ve (A) Upstre m promoters (B) Downstre m promoters (C) Intr ge nic promoters (D) No promoters 330. All of the following st tements out tRNA re true except (A) It is synthesized s l rge precursor (B) It is processed in the nucelolus (C) It h s no codons or nticodons (D) Genes for rRNA re present in single copies 331. Anticodons re present on (A) Coding str nd of DNA (B) mR NA (C) tRNA (D) rRNA 332. Codons re present on (A) (B) (C) (D) Non-coding str n d of DNA hnRNA tRNA None of these MCQs IN BIOCHEMISTRY (C) They re identic l in nucle r nd mitochondri l DNA (D) They h ve no complem ent ry nticodons 337. A polycistronic mRNA c n e seen in (A) Prok ryotes (C) M itochondri (B) Euk ryotes (D) All of these 338. Non-coding sequence re present in the genes of (A) B cteri (B) Viruses (D) All of these 339. Non-coding sequences in nsense codons (D) Exons
gene
re known
(C) Euk ryotes
s (A) Cistrons (C) Introns (B) No
340. Splice sites re present in (A) Prok ryotic mRNA (B) Euk ryotic mRNA (C) Eu k ryotic hnRNA (D) All of these 341. The common fe tures of introns include ll the following except (A) The se sequence egins with GU (B) The se sequence ends with AG (C) The termin l AG sequence is preceded y purine rich tr ct of ten nucleotides (D) An denosine residue in r nch site p rticip tes in splicing 342. A splice some cont ins ll the following except (A) hnRNA (C) Some protein s (A) (B) (C) (D) (A) (B) (C) (D) (B) snRNAs (D) Ri osome 333. Nonsense codons
re present on (A) mRNA (C) rRNA (B) tRNA (D) None of these
334. Genetic code is s id to e degener te ec use (A) It c n undergo mut tions (B) A l rge proportion of DNA is non-coding (C) One codon c n code for more th n one mino cids (D) More th n one codons c n code for the s me mino cids 335. All the following st tements out genetic code re correct except (A) It is de gener te (B) It is un m igous (C) It is ne rly univers l(D) It is overl pping 33 6. All of the following st tements out nonsense codons re true except (A) The y do not code for mino cids (B) They ct s ch in termin tion sign ls 343. Self-splicing c n occur in Some precursors of rRNA Some precursors of tRNA hnRNA None of these Prok ryotic promoters Euk ryotic promoters Both (A) nd (B) None of these 344. Pri now
ox is present in
345. Hogness
ox is present in (A) Prok ryotic promoters
NUCLEIC ACIDS (B) Euk ryotic promoters (C) Both (A) nd (B) (D) None of these 346. CAAT ox is present in (A) Prok ryotic promoters 10 p upstre m of tr nscription st rt site (B) Prok ryotic promoters 35 p upstre m of tr nscription st rt site (C) Euk ry otic promoters 25 p upstre m of tr nscription st rt site (D) Euk ryotic promote rs 70–80 p upstre m of tr nscription st rt site 347. Euk ryotic promoters cont in (A) TATA ox 25 p upstre m of tr nscription st rt site (B) CAAT ox 70-80 p up stre m of tr nscription st rt site (C) Both (A) nd (B) (D) None of these 348. A ll the following st tements out tRNA re correct except (A) A given tRNA c n e ch rged with only one p rticul r mino cid (B) The mino cid is recognized y the nticodon of tRNA (C) The mino cid is tt ched to end of tRNA (D) The n ticodon of tRNA finds the complement ry codon on mRNA 349. All the following st tements out ch rging of tRNA re correct except (A) It is c t lysed y mino cyl tRNA synthet se (B) ATP is converted into ADP nd Pi in this re ction (C) Th e enzyme recognizes the tRNA nd the mino cid (D) There is sep r te enzyme f or e ch tRNA 350. All the following st tements out recognition of codon on m RNA y n nticodon on tRNA re correct except (A) The recognition of the third se of the codon is not very precise (B) Imprecise recognition of the third s e results in wo le (C) Wo le is p rtly responsi le for the degener cy of the g enetic code 257 (D) Wo le results in incorpor tion of incorrect mino cids in the protein 351. The first mino cyl tRNA which initi tes tr nsl tion in euk ryotes is (A) (B) (C) (D) Mehtionyl tRNA Formylmethionyl tRNA Tyrosinyl tRNA Al nyl tRNA 352. The first mino cyl tRNA which initi tes tr nsl tion in prok ryotes is (A) (B) (C) (D) Mehtionyl tRNA Formylmethionyl tRNA Tyrosinyl tRNA Al nyl tRNA 353. In euk ryotes, the 40 S pre-initi tion complex cont ins ll the following i niti tion f ctors except (A) eIF-1A (C) eIF-3 (B) eIF-2 (D) eIF-4 354. Euk ryotic initi tion f ctors 4A, 4B nd 4F ind to (A) (B) (C) (D) 40 S ri osom l su unit 60 S ri osom l su unit mRNA Amino cyl tRNA 355. The codon which serves AG (D) UAA
s tr nsl tion st rt sign l is (A) AUG (C) UGA (B) U
356. The first mino cyl tRNA ppro ches 40 S ri osom l su unit in ssoci tion with (A) eIF-1A nd GTP (C) eIF-2C nd GTP (B) eIF-2 nd GTP (D) eIF-3 nd GTP 357. eIF-1A nd eIF-3 re required (A) For inding of mino cyl tRNA to 40 S ri osom l su unit (B) For inding of mRNA to 40 S ri osom l su unit (C) For indin g of 60 S su unit to 40 S su unit (D) To prevent inding of 60 S su unit to 40 S su unit
258 358. eIF-4 A possesses (A) ATP se ctivity (C) Helic se ctivity 359. eIF-4 B (A ) (B) (C) (D) Binds to 3’ ch in initi tion codon on mRNA Binds to 3’ end of mRNA Bin ds to 5’ end of mRNA Unwinds mRNA ne r its 5’ end (B) GTP se ctivity (D) None of th ese MCQs IN BIOCHEMISTRY 366. Puromycin c uses prem ture ch in termin tion in (A) Prok ryotes (C) Both (A ) nd (B) (B) Euk ryotes (D) None of these 367. Diphtheri toxin inhi its (A) Prok ryotic EF-1 (C) Euk ryotic EF-1 (B) Prok ryotic EF-2 (D) Euk ryotic EF-2 360. Peptidyl tr nsfer se ctivity is present in (A) (B) (C) (D) 40 S ri osom l su unit 60 S ri osom l su unit eEF-2 Amino cyl tRNA 368. The proteins destined to e tr nsported out of the cell h ve ll the follow ing fe tures except (A) They possess sign l sequence (B) Ri osomes synthesizin g them re ound to endopl smic reticulum (C) After synthesis, they re delivere d into Golgi pp r tus (D) They re t gged with u iquitin 369. SRP receptors inv olved in protein export re present on (A) (B) (C) (D) Ri osomes Endopl smic ret iculum Golgi pp rtus Cell mem r ne 361. After form tion of y (A) (B) (C) (D) eEF-1 idyl tr nsfer se nd ATP
peptide ond, mRNA is tr nsloc ted long the ri osome nd GTP eEF-2 nd GTP Peptidyl tr nsfer se nd GTP Pept
362. Binding of formylmehtionyl tRNA to 30 S ri osom l su unit of prok ryotes is inhi ited y (A) Streptomycin (C) Erythromycin (B) Chlor mphenicol (D) Mitomyci n 370. The sign l sequence of proteins is cle ved off (A) (B) (C) (D) On the ri os omes immedi tely fter synthesis In the endopl smic reticulum During processing in Golgi pp r tus During p ss ge through the cell mem r ne 363. Tetr cyclines inhi it inding of mino cyl tRNAs to (A) (B) (C) (D) 30 S r i osom l su units 40 S ri osom l su units 50 S ri osom l su units 60 S ri osom l su units 371. The h lf-life of protein depends upon its (A) (B) (C) (D) Sign l sequence N-terminus mino cid C-terminus mino cid Prosthetic group 364. Peptidyl tr nsfer se ctivity of 50 S ri osom l su units is inhi ited y (A ) Rif mpicin (B) Cycloheximide (C) Chlor mphenicol (D) Erythromycin 365. Erythro mycin inds to 50 S ri osom l su unit nd (A) (B) (C) (D) Inhi its inding of mino cyl tRNA Inhi its Peptidyl tr nsfer se ctivity Inhi its tr nsloc tion C u ses prem ture ch in termin tion 372. Besides structur l genes th t encode sequences which re known s (A) Operons D) Tr ns- cting f ctors 373. Inducers nd er elements respectively (B) Tr ns- cting
proteins, DNA cont ins some regul tory (B) Cistrons (C) Cis- cting elements ( repressors re (A) Enh ncer nd silenc f ctors
NUCLEIC ACIDS (C) Cis- cting elements (D) Regul tory proteins 374. cis- cting elements include (A) Steroid hormones (B) C lcitriol (C) Histones 375. Silencer elements (A) Are tr ns- cting f ctors (B) Are present etween promoters nd the structur l genes (C) Decre se the expression of some structur l genes (D) Encode specific repres sor proteins 376. tr ns- cting f ctors include (A) Promoters (C) Enh ncers (B) R epressors (D) Silencers (D) Silencers 381. L c operon is cluster of (A) Three structur l genes 259 (B) Three structur l genes nd their promoter (C) A regul tory gene, n oper tor nd promoter (D) A regul tory gene, n oper tor, promoter nd three structu r l genes 382. The regul tory i gene of l c operon (A) Is inhi ited y l cotse ( B) Is inhi ited y its own product, the repressor protein (C) Forms regul tory protein which incre ses the expression of downstre m structur l genes (D) Is co nstitutively expressed 383. RNA polymer se holoenzyme inds to l c operon t the following site: (A) i gene (C) Oper tor locus (B) z gene (D) Promoter region 377. Enh ncer elements h ve ll the following fe tures except (A) They incre se gene expression through promoter (B) E ch enh ncer ctiv tes specific promot er (C) They m y e loc ted f r w y from the promoter (D) They m y e upstre m o r downstre m from the promoter 378. Amplific tion of dihydrofol te reduct se gen e m y e rought out y (A) High concentr tions of folic cid (B) Deficiency o f folic cid (C) Low concentr tion of thymidyl te (D) Amethopterin 379. Proteins which inter ct with DNA nd ffect the r te of tr nscription possess the follow ing structur l motif: (A) Helix-turn-helix motif (B) Zinc finger motif (C) Leuci ne zipper motif (D) All of these 380. L c operon is cluster of genes present i n (A) Hum n eings (C) L m d ph ge (B) E. coli (D) All of these 384. Tr ncription of z, y nd genes of l c operon is prevented (C) Repressor (B) Allo-l ctose (D) cAMP
y (A) L ctose
385. Tr nscription of structur l genes of l c operon is prevented y inding of the repressor tetr mer to (A) i gene (C) Promoter (B) Oper tor locus (D) z gene 386. The enzymes encoded y z, y nd genes of l c operon re induci le, nd th eir inducer is (A) L ctose (B) Allo-l ctose (C) C t olite gene ctiv tor protei n (D) All of these 387. Binding of RNA polymer se holoenzyme to the promoter reg ion of l c operon is f cilit ted y (A) C t olite gene ctiv tor protein (CAP) (B) cAMP (C) CAP-cAMP complex (D) None of these
260 388. L ctose or its n logues ct s positive regul tors of l c operon y (A) At t ching to i gene nd preventing its expression (B) Incre sing the synthesis of c t olite gene ctiv tor protein (C) Att ching to promoter region nd f cilit t ing the inding of RNA polymer se holoenzyme (D) Binding to repressor su units s o th t the repressor c nnot tt ch to the oper tor locus 389. Expression of stru ctur l genes of l c operon is ffected y ll the following except (A) (B) (C) ( D) L ctose or its n logues Repressor tetr mer cAMP CAP-cAMP complex MCQs IN BIOCHEMISTRY (B) DNA polymer se I (C) DNA lig se (D) All of these 396. Xeroderm pigmentosum results from defect in (A) (B) (C) (D) uvr ABC excinucle se DNA polymer se I D NA lig se All of these 397. All the following st tements out xeroderm pigmentosum re true except (A ) It is genetic dise se (B) Its inherit nce is utosom l domin nt (C) uvr ABC excinucle se is defective in this dise se (D) It results in multiple skin c ncer s 398. Su stitution of n denine se y gu nine in DNA is known s (A) Tr nspo sition (C) Tr nsversion (B) Tr nsition (D) Fr meshift mut tion 390. The coding sequences in l c operon include (A) (B) (C) (D) (A) (B) (C) (D) i gene i gene, oper tor locus nd promoter z, y nd genes i, z, y nd genes Ultr violet r di tion Ionising r di tion Alkyl ting gents All of these (B) Dime thyl sulph te (D) All of these 391. Mut tions c n
e c used
y
399. Su stitution of thymine se y denine in DNA is known tion (C) Tr nsversion (B) Tr nsition (D) Fr meshift mut tion
s ((A) Tr nsposi
400. A point mut tion results from (A) (B) (C) (D) Su stitution of ion of se Deletion of se All of these 392. Mut tions c n
e c used
se Insert
y (A) Nitros mine (C) Acridine
393. Nitros mine c n de min te (A) (B) (C) (D) Cytosine to form ur cil Adenine t o form x nthine Gu nine to form hypox nthine All of these 401. Su stitution of se c n result in (A) Silent mut tion (B) Mis-sense mu t tion (C) Nonsense mut tion (D) All of these 402. A silent mut tion is most lik ely to result from (A) Su stitution of the first se of codon (B) Su stitutio n of the third se of codon (C) Conversion of nonsense codon into sense c odon (D) Conversion of sense codon into nonsense codon 403. The effect of mis-sense mut tion c n e (A) Accept le (C) Un ccept le (B) P rti lly ccept le (D) All of these 394. Exposure of DNA to ultr violet r di tion c n le d to the form tion of (A) A denine dimers (C) Thymine dimers (B) Gu nine dimers (D) Ur cil dimers 395. D m ge to DNA c used C excinucle se
y ultr violet r di tion c n
e rep ired
y (A) uvr AB
NUCLEIC ACIDS 404. Amino cid sequence of the encoded protein is not ch nged in (A) (B) (C) (D ) Silent mut tion Accept le mis-sense mut tion Both (A) nd (B) None of these 261 (B) Ch nge in re ding fr me of downstre m structur l gene (C) Decre sed efficien cy of tr nscription (D) All of these 412. Mitochondri l protein synthesis is inh i ited y (A) Cycloheximide (C) Diptheri toxin (B) Chlor mphenicol (D) None of these 405. H emoglo in S is n ex mple of / n (A) (B) (C) (D) Silent mut tion Accept le mis-sense mut tion Un ccept le mis-sense mut tion P rti lly ccept le missense mut tion 413. All of the following st tements out puromycin re true except (A) It is n l nyl tRNA n logue (B) It c uses prem ture termin tion of protein synthesis (C) It inhi its protein synthesis in prok ryotes (D) It inhi its protein synthes is in euk ryotes 414. Leucine zipper motif is seen in some helic l proteins when leucine residues ppe r t every (A) 3rd position (C) 7th position (B) 5th posi tion (D) 9th position 406. If the codon UAC on mRNA ch nges into UAG s result of se su stitutio n in DNA, it will result in (A) (B) (C) (D) Silent mut tion Accept le mis-sense mut tion Nonsense mut tion Fr meshift mut tion 407. Insertion of se in gene c n c use (A) Ch nge in re ding fr me (B) G r led mino cid sequence in the encoded protein (C) Prem ture termin tion of tr nsl tion (D) All of these 408. A fr meshift mut tion ch nges the re ding fr me ec use the genetic code (A) (B) (C) (D) Is degener te Is overl pping H s no punc tu tions Is univers l 415. Zinc finger motif is formed in some proteins y inding of zinc to (A) (B) (C) (D) Two cysteine residues Two histidine residues Two rginine residues Two c ysteine nd two histidine residues or two p irs of two cysteine residues e ch 416. Restriction endonucle ses teri (D) All of these
re present in (A) Viruses (C) Euk ryotes (B) B c
409. Suppressor mut tions occur in (A) Structur l genes (B) Promoter regions (C) Silencer elements (D) Anticodons of tRNA 410. Suppressor tRNAs c n neutr lize t he effects of mut tions in (A) Structur l genes (B) Promoter regions (C) Enh nce r elements (D) All of these 411. Mut tions in promoter regions of genes c n c us e (A) Prem ture termin tion of tr nsl tion 417. Restriction endonucle ses split (A) (B) (C) (D) RNA Single str nded DNA Dou le str nded DNA DNA-RNA hy rids 418. Restriction endonucle ses c n recognise (A) (B) (C) (D) P lindromic sequenc es Chimeric DNA DNA-RNA hy rids Homopolymer sequences
262 419. All of the following st tements out restriction endonucle ses re true ex cept: (A) (B) (C) (D) They re present in cteri They ct on dou le str nded D NA They recognize p lindromic sequences They lw ys produce sticky ends MCQs IN BIOCHEMISTRY (A) Southern lotting (B) Northern lotting (C) Both (A) nd (B) (D) None of the se 427. An nti ody pro e is used in (A) Southern lotting (B) Northern lotting (C) Western lotting (D) None of these 428. A p rticul r protein in mixture c n e detected y A) Southern lotting (B) Northern lotting (C) Western lottin g (D) None of these 429. The first protein synthesized y recom in nt DNA techno logy w s (A) (B) (C) (D) Streptokin se Hum n growth hormone Tissue pl sminogen ctiv tor Hum n insulin 420. Which of the following is
p lindromic sequence (A) (B) (C) (D)
5′ − ATGCAG − 3′ 3′ − TACGTC − 5′ 5′ − CGAAGC − 3′ 3′ − GCTTCG − 5′ 421. In stic y ends produced by restriction endonuc eases (A) The 2 strands of D NA are joined to each other (B) The DNA strands stic to the restriction endonuc ease (C) The ends of a doub e stranded fragment are over apping (D) The ends of a doub e stranded fragment are non over apping 422. A of the fo owing may be used as expression vectors except (A) P asmid (C) Bacu ovirus 423. A p asmid is a (A) (B) (C) (D) Sing e stranded inear DNA Sing e stranded circu ar DNA Doub e stranded inear DNA Doub e stranded circu ar DNA (B) Bacteriophage (D) E. co i 430. For production of eu aryotic protein by recombinant DNA techno ogy in bacte ria, the temp ate used is (A) Eu aryotic gene (C) mRNA (B) hnRNA (D) A of thes e 431. Monoc ona antibodies are prepared by c oning (A) Mye oma ce ocytes (B) Hybridoma ce s (D) B Lymphocytes 432. Mye oma ce s are ase HGPRT A of these
s (C) T Lymph
ac ing in (A) (B) (C) (D) TMP synthetase Formy
transfer
424. Fragments of DNA can be identified by the technique of (A) Western b otting (B) Eastern b otting (C) Northern b otting (D) Southern b otting 425. A particu ar RNA in a mixture can be identified by (A) Western b otting (B) Eastern b ott ing (C) Northern b otting (D) Southern b otting 426. A radioactive isotope abe ed cDNA probe is used in 433. Hybridoma ce s are se ected by cu turing them in a medium containing (A) ( B) (C) (D) Adenine, guanine, cytosine and thymine Adenine, guanine, cytosine and uraci Hypoxanthine, aminopterin and thymine Hypoxanthine, aminopterin and thym idine 434. Mye oma ce s and ymphocytes can be fused by using (A) Ca cium ch oride (B ) Ethidium bromide (C) Po yethy ene g yco (D) DNA po ymerase
NUCLEIC ACIDS 435. Tria s for gene therapy in human beings were first carried out, with consid erab e success, in a genetic disease ca ed (A) (B) (C) (D) (A) (B) (C) (D) Cyst ic fibrosis Tha assemia Adenosine deaminase deficiency Lesch Nyhan syndrome Is f ound in bacteriophages Contains unre ated genes Has no restriction sites Is pa i ndromic (A) E. co i (C) T.aquaticus (B) Yeast (D) Eu aryotes 263 444. Base sequence of DNA can be determined by (A) (B) (C) (D) Maxam Gi bert met hod Sanger’s dideoxy method Both (A) and (B) None of these 436. Chimeric DNA 445. From a DNA RNA hybrid, DNA can be obtained by addition of (A) (B) (C) (D) D NA B protein and ATP He icase and ATP DNA topoisomerase I A a i 437. Which of the fo owing may be used as a c oning vector? (A) Pro aryotic p a smid (B) Lambda phage (C) Cosmid (D) A of these 438. The p asmid pBR322 has (A ) (B) (C) (D) Ampici in resistance gene Tetracyc ine resistance gene Both (A) a nd (B) None of these 446. Optimum temperature of DNA po ymerase of T. aquaticus is (A) 30°C (C) 54°C (B) 37°C (D) 72°C 447. In addition to Taq po ymerase, po ymerase chain reaction requires a of th e fo owing except (A) (B) (C) (D) A temp ate DNA Deoxyribonuc eoside triphospha tes Primers Primase 439. Lambda phage can be used to c one DNA fragments of the size (A) Upto 3 i o bases (B) Upto 20 i obases (C) Upto 45 i obases (D) Upto 1,000 i obases 440. DNA fragments upto 45 i obases in size can be c oned in (A) (B) (C) (D) (A) (B) (C) (D) Bacteria p asmids Lambda phage Cosmids Yeast artificia chromosomes La rge bacteria p asmid Vira p asmid Hybrid of p asmid and phage Yeast p asmid 448. DNA po ymerase of T. aquaticus is preferred to that of E. co i in PCR becau se (A) It rep icates DNA more efficient y (B) It doesn’t require primers (C) It is not denatured at the me ting temperature of DNA (D) It doesn’t cause errors in re p ication 449. Twenty cyc es of PCR can amp ify DNA: (A) 220 fo d (C) 20 x 2 fo d (B) 202 fo d (D) 20 fo d 441. A cosmid is a 442. Po ymerase chain reaction can rapid y amp ify DNA sequences of the size (A) Upto 10 i obases (B) Upto 45 i obases (C) Upto 100 i obases(D) Upto 1,000 i obases 443. The DNA po ymerase common y used in po ymerase chain reaction is ob tained from 450. Transgenic anima s may be prepared by introducing a foreign gene into (A) S omatic ce s of young anima s (B) Testes and ovaries of anima s (C) A vira vect or and infecting the anima s with the vira vector (D) Ferti ised egg and imp an ting the egg into a foster mother
264 451. Yeast artificia chromosome can be used to amp ify DNA sequences of the siz e (A) Upto 10 b (C) Upto 100 b (B) Upto 45 b (D) Upto 1,000 b (A) Cysteine ( C) G utamate (A) G utamate (C) Tryptophan (A) Va ine (C) Iso eucine (A) Homoseri ne (C) Tyrosine MCQs IN BIOCHEMISTRY (B) Aspartate (D) A of these (B) Histidine (D) Methionine (B) Leucine (D) A of these (B) Homocysteine (D) Tryptophan 459. N Formiminog utamate is a metabo ite of 452. DNA finger printing is based on the presence in DNA of (A) (B) (C) (D) Cons tant number of tandem repeats Variba e number of tandem repeats Non repititive s equences in each DNA Introns in eu aryotic DNA 460. Methy ma ony
CoA is a metabo ite of
461. Homogentisic acid is formed from 453. A the fo owing statements about restriction fragment ength po ymorphism are true except (A) It resu ts from mutations in restriction sites (B) Mutation s in restriction sites can occur in coding or non coding regions of DNA (C) It i s inherited in Mende ian fashion (D) It can be used to diagnose any genetic dise ase 454. Inborn errors of urea cyc e can cause a the fo owing except (A) Vomi ting (C) Rena fai ure (B) Ataxia (D) Menta retardation 462. Map e syrup urine disease resu ts from absence or serve deficiency of (A) ( B) (C) (D) Homogentisate oxidase Pheny a anine hydroxy ase Branched chain amino acid transaminase None of these 463. Which of the fo owing is present as a mar er in ysosoma enzymes to direc t them to their destination? (A) (B) (C) (D) G ucose 6 phosphate Mannose 6 phosp hate Ga actose 6 phosphate N Acety neuraminic acid 455. Hyperammonaemia type I resu ts from congenita absence of (A) (B) (C) (D) G utamate dehydrogenase Carbamoy phosphate synthetase Ornithine transcarbamoy as e None of these 464. Marfan’s syndrome resu ts from a mutation in the gene coding: (A) Co ) Fibri in (B) E astin (D) Keratin
agen (C
456. Congenita deficiency of ornithine transcarbamoy ase causes (A) (B) (C) (D) Hyperammonaemia type I Hyperammonaemia type II Hyperornithinaemia Citru inaemi a 465. A the fo owing statements about fibronectin are true except (A) (B) (C) (D) It is g ycoprotein It is a trip e he ix It is present in extra ce u ar matr ix It binds with integrin receptors of ce 457. A etogenic amino acid among the fo Serine (D) Pro ine
owing is (A) Leucine (C) Threonine (B)
466. Fibronectin has binding sites for a of the fo in (C) Heparin (B) Co agen (D) Integrin receptor 458. Carbon s e eton of the fo uconeogenesis
owing except (A) G ycophor
owing amino acid can serve as a substance for g
467. Fibronectin is invo ved in (A) Ce
adhension (C) Both (A) and (B) (B) Ce
movement (D) None of these
NUCLEIC ACIDS 468. G ycoproteins are mar ed for destruction by remova of their (A) (B) (C) (D ) O igosaccharide prosthetic group Sia ic acid residues Mannose residues N termi na amino acids 265 476. A the fo owing statements about protooncogenes are true except (A) (B) ( C) (D) They are present in human beings They are present in hea thy ce s Protei ns encoded by them are essentia They are expressed on y when a hea thy ce has been transformed into a cancer ce 469. G ycophorin is present in ce s (B) P ate ets (D) Liver
membranes of (A) Erythrocytes (C) Neutrophi
477. Various oncogens may encode a of the fo owing except: (A) (B) (C) (D) Ca rcinogens Growth factors Receptors for growth factors Signa transducers for gro wth factors 470. Se ectins are proteins that can recognise specific (A) Carbohydrates (C) Am ino acids (B) Lipids (D) Nuc eotides 471. Hunter’s syndrome resu ts from absence of (A) (B) (C) (D) Hexosaminidase A Id uronate su phatase Neuraminidase Ary su phatase B 478. Ras proto oncogene is converted into oncogene by (A) A point mutation (B) C hromosoma trans ocation (C) Insertion of a vira promoter upstream of the gene (D) Gene amp ification 479. Ras proto oncogene encodes (A) (B) (C) (D) Epiderma growth factor (EGF) Receptor for EGF Signa transducer for EGF Nuc ear transcri ption factor 472. A cancer ce is characterized by (A) (B) (C) (D) Uncontro ed ce n Invasion of neighbouring ce s Spread to distant sites A of these
divisio
473. If DNA of a cancer ce is introduced into a norma ce , the recipient ce (A) (B) (C) (D) Destroys the DNA Loses its abi ity to divide Dies Changes into a cancer ce 480. P 53 gene: (A) A proto oncogene (B) An oncogene (C) A tumour suppressor gen e (D) None of these 481. Retinob astoma can resu t from a mutation in (A) ras pr oto oncogene (B) erbB proto oncogene (C) p 53 gene (D) RB 1 gene 482 A the fo owing statements about retino b astoma are true except (A) At east two mutatio ns are required for its deve opment (B) One mutation can be inherited from a par ent 474. A norma ce can be transformed into a cancer ce by a of the fo owing except (A) (B) (C) (D) Ionising radiation Mutagenic chemica s Oncogenic bacteri a Some viruses 475. Proto oncogens are present in (A) (B) (C) (D) Oncoviruses Cancer ce thy human ce s Pro aryotes
s Hea
266 (C) Chi dren who have inherited one mutation deve op retinob astoma at a younger age (D) RB 1 gene promotes the deve opment of retinob astoma 483. Ames assay is a rapid method for detection of (A) Oncoviruses (B) Retroviuses (C) Chemica ca rcinogens (D) Typhoid 484. Amp ification of dihydrofo ate reductase gene in a ca ncer ce ma es the ce (A) Susceptib e to fo ic acid deficiency (B) Less ma ig nant (C) Resistant to amethopterin therapy (D) Responsive to amethopterin therap y 485. Conversion of a procarcinogen into a carcinogen often requires (A) Proteo ysis (B) Microsoma hydroxy ation (C) Exposure to u travio et radiation (D) Exp osure to X rays 486. The on y correct statement about oncoviruses is (A) A the oncoviruses are RNA viruses (B) Reverse transcriptase is present in a oncovir uses (C) Vira oncogenes are identica to human protooncogens (D) Both DNA and R NA viruses can be oncoviruses 487. RB 1 gene is (A) A tumour suppressor gene (B) Oncogene (C) Proto oncogene (D) Activated proto oncogene 488. Cancer ce s may become resistant to amethopterin by (A) Deve oping mechanisms to destroy amethop terin MCQs IN BIOCHEMISTRY (B) Amp ification of dihydrofo ate reducatse gene (C) Mutation in the dihydrofo ate reductase gene so that the enzyme is no onger inhibited by amethopterin (D) Deve oping a ternate pathway of thymidy ate synthesis 489. The major source of NH3 produced by the idney is (A) Leucine (C) A anine (B) G ycine (D) G utamine 490. Which of these methy donors is not a quanternary ammonium compound? (A) Me thionine (C) Betain (B) Cho ine (D) Betaina dehyde 491. L g utamic acid is subjected to oxidative deaminition by (A) (B) (C) (D) L amino acid dehydrogenase L g utamate dehydrogenase G utaminase G utamine synthet ase 492. A pro aryotic ribosome is made up of ________ sub units. (A) 20 S and 50 S (C) 30S and 60S (B) 30S and 50S (D) 20S and 50S 493. AN Eu aryotic ribosome is made up of ________ sub unit. (A) 40S and 60S (C) 40S and 80S (A) (B) (C) (D) (B) 40S and 50S (D) 60S and 80 S 494. GTP is not required for Capping L of mRNA Fusion of 40S and 60S of ribosome Accommodation of tRNA amino acid Formation of tRNA amino acid comp ex 495. The antibiotic which inhibits DNA dependent RNA po ymerase is (A) Mitomycin C (C) Streptomycin (A) Actinomycin d (C) Puromycin (B) Actinomycin d (D) Puromy cin (B) Streptomycin (D) Mitomycin C 496. The antibiotic which c eaves DNA is
NUCLEIC ACIDS 497. The antibiotic which has a structure simi ar to the amino acy end of tRNA tyrosine is (A) Actinomycin d (C) Puromycin 498. ATP is required for (A) Fusion of 40S and 60 S of ribosome (B) Accommodation tRNA amino acid in a site of ribos ome (C) Movement of ribosome a ong mRNA (D) formation of tRNA amino acid comp ex 499. What is the subce u ar site for the biosynthesis of proteins? (A) Chromos omes (C) Ribosomes (B) Lymosomes (D) Centrosomes (B) Streptomycin (D) Mitomycin c 267 506. Progressive transmethy ation of ethano amine gives (A) (B) (C) (D) Creatini ne Cho ine Methionine N methy nicotinamide 507. Genetic information srcinates from (A) (B) (C) (D) (A) (B) (C) (D) Cistron of DNA Codons of mRNA Anticodons of tRNA Histones of nuc eoproteins The protein moiety of DNA Cistrom of DNA Nuc eotide sequence of m RNA The anticodons of tRN A 508. The genetic code operates through 500. An anima is in negative nitrogen ba ance when (A) (B) (C) (D) Inta e excee ds output New tissue is being synthesized Output exceeds inta e Inta e is equa to output 509. DNA synthesis in aboratory was first achieved by (A) Watson and cric (B) Khorana (C) A.Kornberg (D) Ochoa 510. Among the different types of RNA, which on e has the highest M.W.? (A) mRNA (C) yeast RNA (B) rRNA (D) tRNA 501. When NH3 is perfused through a dog’s iver ______ is formed, whi e ______ is formed in the birds iver. (A) Urea, Uric acid (B) Urea, a antoin (C) Uric acid , creatinine (D) Uric acid, Urea 502. Aspartate amino transferase uses the fo o wing for transamination: (A) (B) (C) (D) G utamic acid and pyruvic acid G utamic acid and oxa oacetic acid Aspartic acid and pyruvic acid aspartic acid and eto adipic acid 511. From DNA the genetic message is transcribed into this compound: (A) Protein (C) tRNA (B) mRNA (D) rRNA 512. This compound has a doub e he ica structure. (A) Deoxyribonuc eic acid (B) RNA (C) F avine adevine dinuc eotide (D) Nicotinamide adamine dinuc eotide 513. The structura stabi ity of the doub e he ix of DNA is as cribbed arge y to (A ) Hydrogen bonding between adjacent purine bases (B) Hydrophobic bonding between sta ed purine and pyrinuidine nuc ei 503. Which among the fo owing compounds is not a protein? (A) Insu in (C) Mucin (A) Kidney (C) Uterus (B) Hheparin (D) Pepsin (B) Urethra (D) Liver 504. A most a
the urea is formed in this tissue:
505. A po yribosome wi B) 10 (D) 2
have about _______ individua
ribosomes. (A) 20 (C) 5 (
268 (C) Hydrogen bonding between adjacent pyrimidine bases (E) Hydrogen bonding betw een purine and pyrimidine bases 514. Which of the fo owing statements about nuc eic acid is most correct? (A) Both pentose nuc eic acid and deoxypentose nuc ei c acid contain the same pyrimidines (B) Both pentose nuc eic acid and deoxypento se nuc eic acid and deoxypentose nuc eic acid Contain the same purines (C) RNA c ontains cytosine and thymine (D) DNA and RNA are hydro ysed by wea a a i 515. Acid hydro ysis of ribonuc eic acid wou d yie d the fo owing major products: (A ) (B) (C) (D) d deoxyribose, cytosine, adenine d ribose, thymine, Guanine d rib ose, cytosine, uraci , thymine d ribose, uraci , adenine, guanine, cytosine MCQs IN BIOCHEMISTRY 520. Which of the fo owing contains a deoxy sugar? (A) RNA (C) ATP 521. DNA is (A) Usua y present in tissues as a nuc eo protein and cannot be separated from its protein component (B) A ong chain po ymer in which the internuc eotide in ages are of the diester type between C 3’ and C 5’ (C) Different from RNA since in t he atter the internuc eotide in ages are between C 2’ and C 5’ (D) Hydro yzed by w ea a a i (pH9 to 100°C) 522. Nobody is the name given to (A) Ribosome (C) Centro some (A) (B) (C) (D) (B) Microsome (D) Nuc eosome (B) DNA (D) UTP 523. Transcription is the formation of DNA from a parent DNA mRNA from a parent mRNA pre mRNA from DNA protein through mRNA 516. RNA does not contain (A) adenine (C) d ribose (B) OH methy aci
cytosine (D) Ur
517. Which of the fo owing statements is correct? (A) a nuc eo protein usua y contain deoxy sugars of the hexose type (B) Nuc eoproteins are usua y absent fr om the cytop asm (C) Nuc eoproteins usua y are present in the nuc eus on y (D) Nuc eoproteins usua y occur in the nuc eus and cytop asm 518. Whcih of the fo owing compound is present in RNA but absent from DNA? (A) Thymine (C) Uraci (B) Cytosine (D) Guanine 524. Trans ation is the formation of (A) (B) (C) (D) DNA from DNA mRNA from DNA Protein through mRNA mRNA from pre mRNA 525. Sigma and Rho factors are required for (A) Rep ication (C) Trans ation (B) Transcription (D) Po ymerisation 526. The genine of φ ×174 bacteriophage is interesting in that i
contains (A) (B) ( C) (D) No DNA DNA with uracil Single stranded DNA Triple standard DNA 519. Nucleic acids can be detected by means o their absorption maxima near 260 nm. Their absorption in this range is due to (A) (B) (C) (D) Proteins Purines an d pyrimidines Ribose Deoxyribose 527. Okasaki ragments are small bits o ads RNA with DNA heads
(A) (B) (C) (D) RNA DNA DNA with RNA he
NUCLEIC ACIDS 528. In addition to the DNA o nucleus there DNA is (A) (B) (C) (D) (A) (B) (C) (D) Mitochondrian Endoplasmic reticulum Golgi apparatus Plasma membrane Like the nuclear DNA in structure Single stranded, linear Double stranded, circular Sing le stranded, circular Q536. RNA synthesis requires (A) RNA primer (C) DNA templa te (B) RNA template (D) DNA primer 269 537. The mRNA ready (B) CTP (D) UTP
or protein synthesis has the ________ cap. (A) ATP (C) GTP
529. The mitochondrial DNA is 538. mRNA ready A (D) C
or protein synthesis has the poly _______ toil. (A) G (C) U (B)
530. A synthetic RNA having the sequence o UUUUUU (Poly U) will give a protein having poly ______. (A) Alamine (C) Glycine (B) Phenyl alanine (D) Methionine 539. The codon
or phenyl Alanine is (A) AAA (C) GGG (B) CCC (D) UUU
531. Lac operon o E. coli contains _______ is continuity. (A) (B) (C) (D) (A) ( B) (C) (D) (A) (B) (C) (D) (A) (B) (C) (D) (A) (B) (C) (D) Regulator and operato r genes only Operator and structural genes only Regular and structural genes onl y Regulator, operator and structural genes Only one polypeptide Two polypeptides Three polypeptides Five polypeptides More than one polypeptide Only one polypep tide Many exons and introns Introns only Replicase Transcriptase Reverse transcr iptase Polymerase III Replicase Transcriptase Reversetranscriptase Polymerase–III 540. Blue print or genetic in ormation residues in (A) mRNA (C) rRNA 541. Genes are (A) RNA (C) lipoproteins and 542. Codons are in (A) DNA (C) tRNA (B) mRNA ( D) rRNA (B) DNA (D) Chromoproteins (B) tRNA (D) DNA 532. A mRNA o 533. mRNA o
eukaryotes can code prokaryotes can code
or or
543. The genetic code operates via (A) The protein moiety o DNA (B) The base se quences o DNA (C) The nucleotide sequence o mRNA (D) The base sequence o tRNA 544. Urine bases with methyl substituents occurring in plants are (A) Ca eine (C) Theobromine (B) Theophylline (D) All o these 534. DNA directed RNA polymerase is 535. RNA directed DNA polymerase is 545. Genetic in ormation in human beings is stored in (A) DNA (C) Both (A) and ( B) (B) RNA (D) None o these
270 546. All ollowing are naturally occurring nucleotides except (A) Cyclic AMP (B) ATP (C) DNA (D) Inosine monophosphate 547. I the amino group and a carboxylic group o the amino acid are attached to same carbon atom, the amino acid is call ed as (A) Alpha (C) Gamma (B) Beta (D) Epsilon (A) Deoxyribose (C) Adenine MCQs IN BIOCHEMISTRY (B) Uracil (D) Thymine 555. Which o the ollowing are nucleo proteins? (A) (B) (C) (D) Protamines Hist ones Deoxy and Ribo nucleo proteins All o these 556. The total RNA in cell tRNA constitutes (A) 1–10% (C) 30–50% (B) 10–20% (D) 50–80% 557. Unit o
genetic in ormation: (A) DNA (C) Cistron (B) RNA (D) None o
these
548. I in a nucleic acid there are more than 8000 nucleotides it is most likely (A) RNA (C) Both (A) and (B) (B) DNA (D) None o these 558. Anticodon sequence are seen in (A) tRNA and transcribed DNA strand (B) tRNA and complementary DNA strand (C) mRNA (D) mRNA and complementary DNA strand 559 . cAMD is destroyed by (A) (B) (C) (D) Adenylate cyclase Phosphodiesterase Synth etase phosphatase Synthetase kinase (B) Birds (D) Bacteriophase (B) Lipic acid ( D) Biotin 549. Genetic in ormation in human beings is stored in (A) RNA (C) Both (A) and ( B) (B) DNA (D) mRNA 550. In RNA, apart rom ribose and phosphate, all A) Adenine (C) Thymine (B) Guanine (D) Cytosine
ollowing are present except (
551. Which o the ollowing gives a positive Ninhydrin test? (A) Reducing sugar (C) α- mino cids 552. A Gene is (A) (B) (C) (D) A single protein molecule A group
of chromosomes An instruction for m king le (B) Triglycerides (D) Phospholipids 560. Restriction enzymes h ve (C) Thymine
protein molecule A
it of DNA molecu
een found in (A) Hum ns (C) B cteri
(A) Thi mine
561. Sulphur is not present in 562. Which one of the following inds to specific nucleotide sequences? (A) RNA polymer se (B) Repressor (C) Inducer (D) Restriction 563. Using written converti on which one of the following sequences is compliment ry to TGGCAGCCT? (A) ACC G TC GGA (C) AGG CTG CCA (B) ACC GUC GGA (D) TGG CTC GGA 553. In DNA, genetic inform tion is loc ted in (A) Purine ses (B) Pyrimidine ses (C) Purine nd pyrimidine ses (D) sug r 554. Which one of the following i s not constituent of RNA? 564. Ri osomes simil r to those of
cteri l found in
NUCLEIC ACIDS (A) (B) (C) (D) Pl nt nucei C rdi c muscle cytopl sm Liver endopl smic reticulum Neuron l cytopl sm (A) (B) (C) (D) (A) (B) (C) (D) Ri ose 5 phosph te Phosphori osyl pyrophosph te Hypox nthine Adenosine Atmospheric CO2 1 c r on c rried y f ol te Betoine Methionine (B) Purines (D) Pyridoxine 271 565 The mech nism of synthesis of DNA nd RNA re simil r in ll the following w ys except (A) They involve rele se of pyrophosph te from e ch nucleotide dded (B) They require ctiv ted nucleotide precursor nd Mg2+ (C) The direction of sy nthesis is 5’ → 3’ (D) They require primer 566. Templ te-directed DNA synthesis occu rs in ll the following except (A) (B) (C) (D) The replic tion fork Polymer se c h in re ction Growth of RNA tumor viruses Expression of oneogenes 572. C r on 6-of purine skeleton comes from 573. Uric
cid is the c t
olic end product of (A) Porphyrine (C) Pyrimidines
574. Diphenyl mine method is employed in the qu ntit tion of (A) Nucleic ) DNA (B) RNA (D) Proteins 575. Orcinol method is employed in the qu ntit tion of (A) Nucleic (B) DNA (D) Proteins
cid (C
cid (C) RNA
567. Which one of the following st tements correctly descri es euk ryotic DNA? ( A) They involve rele se of pyrophosph te from e ch nucleotide precussor nd Mg2+ (B) The direction of synthesis is (C) They require primer 5’ → 3’ (D) None of these 568. Which one of the following c uses fr me shift mut tion? (A) (B) (C) (D) Tr nsition Tr nsversion Deletion Su stitution of purine to pyrimidine α- l nine β- l n ine α- minoiso utyr te β- minoiso utyr te (B) C4, C5 nd N7 (D) C4, C6 nd N7 576. Nucleic cid show strong sorption t one of the w velength: (A) 280 nm (C ) 360 nm 577. tRNA h s (A) (B) (C) (D) Clover le f structure nticodon rm poly ‘A’ t y 3’ C p t 5’ end (B) 220 nm (D) 260 nm 569. C t
olism of thymidyl te gives (A) (B) (C) (D)
578. Which one of the following contri utes nitrogen toms to oth purine nd py rimidine rings? (A) (B) (C) (D) Asp rt te C r noyl phosph te C r ondioxide Tetr hydrofol te 570. Glycine gives __________
toms of purine. (A) C2, C3 (C) C4, C5
nd N9
579. The four nitrogen toms of purines re derived from (A) (B) (C) (D) Ure n d NH3 NH3, Glycine nd Glut m te NH3, Asp r te nd Glut m te Asp rt te, Glut min e nd Glycine 571. A common su str te of HGPRT se, APRT se is
nd PRPP glut myl
midotr nsfer se
272 580. A drug which prevents uric cid synthesis y inhi iting the enzyme X nthine oxid se is (A) Aspirin (C) Colchicine (B) Allopurin l (D) Phenyl enzo te (A) ( B) (C) (D) MCQs IN BIOCHEMISTRY Glut mine nd C r moyl-p Asp r te nd NH3
nd C r
moyl-p Glut m te
nd NH3 Glut mine
581. Glycine contri utes to the following C nd N of purine nucleus: (A) C1, C2 nd N7 (C) C4, C5 nd N7 (A) (B) (C) (D) (A) (B) (C) (D) (B) C8, C8 nd N9 (D) C 4, C5 nd N9 589. All re true out lesch-nyh n syndrome except (A) (B) (C) (D) Produces sel f-mutil tion Genetic deficiency of the enzyme Elev ted levels of uric cid in l ood Inherit nce is utosom l recessive 582. Insoinic cid is the iologic l precursor of Cytosine nd Uric cid Adenylv e cid nd Glucine floc cid Orotic cid nd Uridylic cid Adenosine cid Thymid ine A defect in excretion of uric cid y kidney An overproduction of pyrimidine s An overproduction of uric cid Rise in c lcium le ding to deposition of c lciu m ur te 590. Synthesis of GMP nd IMP requires the following: (A) (B) (C) (D) NH3 NAD+, ATP Glut mine, NAD+, ATP NH3, GTP, NADP+ Glut mine, GTP, NADP+ 583. The pro
le met
olic defect in gents is
591. Which p thw y is correct for c t olism of purines to form uric cid? (A) G u nyl te→Adenyl te→X nthine→hypox nthine→Uric cid (B) Gu nyl te→inosin te→X nthine→hypox ne→Uric cid (C) Adenyl te→Inosin te→X nthine hypox nthine→Uric cid (D) Adenyl te→Inosin te→hypox nthine X nthine→Uric cid 592. Polysemes do not cont in (A) Protein (C) mRN A (B) DNA (D) rRNA 584. In hum ns, the princip l ne (B) All ntin (D) Uric cid
re k down product of purines is (A) NH3 (C) Al ni
585. A key su st nce in the committed step of pyrimidines iosynthesis is (A) (B ) (C) (D) Ri ose-5-phosph te C r moyl phosph te ATP Glut mine 586. In hum ns, the princip l met olic product of pyrimidines is (A) Uric (C) Hypox nthine (B) All ntoin (D) β- l nine
cid
593. The form tion of peptide ond during the elong tion step of protein synth esis results in the splitting of how m ny high energy onds? (A) 1 (C) 3 (B) 2 ( D) 4 587. In most m mm ls, except prim tes, uric cid is met olized y (A) (B) (C) ( D) Oxid tion to ll ntoin Reduction to NH3 Hydrolysis to ll ntoin Hydrolysis to NH3 594. Tr nsloc se is n enzyme required in the process of (A) DNA replic tion (B) RNA synthesis (C) Initi tion of protein synthesis (D) Elong tion of peptides 59 5. Nonsense codons ring out 588. Two nitrogen of the pyrimidines ring
re o t ined from
NUCLEIC ACIDS (A) Amino cid ctiv tion (B) Initi tion of protein synthesis (C) Termin tion of protein synthesis (D) Elong tion of polypeptide ch ins 596. Which of the follow ing genes of the E.coli “L c operon” codes for constitutive protein? (A) The ‘ ’ gene (C) The ‘c’ gene (B) The ‘i’ gene (D) The ‘z’ gene 273 (D) Multiple codons for single mino cid 603. The norm l function of restrict ion endonucle ses is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from ok z ki fr gments (D) Protect cteri from foreign DNA 604. In contr st to Euk ryotic mRNA, prok ryotic mRNA is ch r cteriz ed y (A) H ving 7-methyl gu nosine triphosph te t the 5’ end (B) Being polycystr onic (C) Being only monocystronic (D) Being synthesized with introns 605. DNA li g se of E. coli requires which of the following co-f ctors? (A) FAD (C) NADP+ (B ) NAD+ (D) NADH 597. In the process of tr nscription, the flow of genetic inform tion is from (A ) DNA to DNA (C) RNA to protein (B) DNA to protein (D) DNA to RNA 598. The nticodon region is mRNA (B) tRNA (D) hrRNA
n import nt p rt of the structure of (A) rRNA (C)
599. The region of the L c operon which must e free from structur l gene tr nsc ription to occur is (A) The oper tor locus (B) The promoter site (C) The ‘ ’ gene (D ) The ‘i’ gene 600. Another n me for reverse tr nscript se is (A) DNA dependent DNA polymer se (B) DNA dependent RNA polymer se (C) RNA dependent DNA polymer se (D) RNA dependent RNA polymer se 601. In the ’l c operon’ concept, which of the followi ng is protein? (A) Oper tor (C) Inducer (B) Repressor (D) Vector 606. Which of the following is tr nscri ed during repression? (A) Structur l gen e (C) Regul tor gene (B) Promoter gene (D) Oper tor gene 607. mRNA is complement ry copy of (A) (B) (C) (D) 5′-3′ str nd of DNA+ 3′-5′ str nd of DNA Antisense str nd of DNA tRNA 608. Synthesis of RNA molecule is termin ted (A) α-f ctor (C) δ-facto (B) β-facto (D)
y
sign l which is recognised
y
602. D g n acy of th g n tic co not th xi t nc of (A) Ba t ipl t t hat o not co fo any amino aci (B) Co on con i ting of only two a (C) Co on that inclu on o mo of th unu ual a 609. Th in ing of p oka yotic DNA p n nt RNA polym a to p omot it of g n i inhi it y th anti iotic: (A) St ptomycin (C) Au omycin (B) Rifam cin (D) Pu omycin 610. In E. coli th chain initiating amino aci in p ot in myl m thionin (B) M thionin (C) S in (D) Cy t in
ynth
i
i
(A) N-fo
274 611. Amanitin th mu h oom poi on inhi it (A) (B) (C) (D) Glycop ot in ATP ynth i DNA ynth i mRNA ynth i
ynth
i
MCQ IN BIOCHEMISTRY up t am an mo t i tant f om th ta t it ? (A) RNA polym a (B) R p o (C) In uc (D) R t iction 618. U ing w itt n conv ntion which on of th follo wing qu nc i complim nta y to TGGCAGCCT? (A) ACCGTCGGA (C) AGGCTGCCA (B) AC CGUCGGA (D) TGGCTCGGA 612. How many high- n gy pho phat on activation in p ot in ynth i ? (A) On 613. T an lation ot in mol cul
ult
in th
619. Ri o om imila to tho i Ca iac mu cl cytopla m Liv
quival nt a qui fo (C) Th (B) Two (D) Fou
fo mation of (A) mRNA (C)
amino aci
RNA (B) tRNA (D) A p
of act ia foun in (A) (B) (C) (D) Plant nucl n opla mic ticulum N u onal cytopla m
614. Elongation of a p pti chain involv all th following xc pt (A) mRNA (B ) GTP (C) Fo myl-M t-tRNA (D) Tu, TS an G facto 615. Th ‘ ho’ (ρ) facto i involv ρ (A) To inc a th at of RNA ynth i (B) In in ing cata olit p o to th p omot gion (C) In p op t mination of t an c iption (D) To allow p op initiation of t an c ipti 616. In th io ynth i of c-DNA, th joinin g nzym liga qui (A) GTP (C) CTP (B) ATP (D) UTP 620. Th m chani m of ynth i of DNA an RNA a imila to all th following way xc pt (A) Th y involv l a of py opho phat f om ach nucl oti a (B) Th y qui activat nucl oti p cu o an Mg2+ (C) Th i ction of ynth i i (D) Th y qui a p im 621. T mplat - i ct DNA ynth i occu in all th following xc pt (A) (B) (C) (D) Th plication fo k Polym a ch ain action G owth of RNA tumo vi u Exp ion of oncog n 617. Which on
of th
following
in
to
p cific nucl oti
qu nc
that a
NUCLEIC ACIDS 275 ANSWERS 1. B 7. B 13. A 19. A 25. C 31. D 37. C 43. B 49. B 55. D 61. B 67. A 73. C 79. B 85. A 91. B 97. B 103. B 109. D 115. B 121. A 127. D 133. D 139. A 145. D 151. D 157. B 163. C 169. C 175. D 181. B 187. B 193. A 199. A 205. C 211. B 217. D 223. A 229. D 235. B 241. B 247. A 2. B 8. D 14. D 20. A 26. A 32. A 38. A 44. C 50. A 56. A 62. C 68. A 74. B 80. A 86. D 92. B 98. B 104. B 110. C 116. A 122. D 128. C 134. A 140. D 146. B 152. A 158. A 164. C 170. D 176. A 182. B 188. C 194. D 200. C 206. D 212. C 218. B 224. C 230. B 236. A 242. C 248. C 3. A 9. C 15. B 21. B 27. C 33. B 39. B 45. D 51. D 57. D 63. A 69. B 75. C 81. C 87. A 93 . A 99. D 105. A 111. D 117. D 123. B 129. A 135. A 141. B 147. B 153. C 159. A 165. D 171. B 177. B 183. C 189. D 195. D 201. D 207. B 213. D 219. D 225. A 231 . C 237. C 243. D 249. A 4. C 10. D 16. A 22. C 28. B 34. A 40. D 46. B 52. B 58 . A 64. A 70. A 76. A 82. A 88. B 94. A 100. A 106. B 112. A 118. A 124. C 130. A 136. D 142. D 148. B 154. A 160. A 166. C 172. B 178. D 184. B 190. A 196. A 2 02. C 208. C 214. C 220. A 226. B 232. A 238. C 244. C 250. D 5. A 11. A 17. C 2 3. C 29. C 35. A 41. C 47. A 53. B 59. A 65. A 71. A 77. C 83. A 89. A 95. A 101 . B 107. C 113. B 119. A 125. A 131. B 137. B 143. C 149. D 155. B 161. C 167. A 173. C 179. D 185. A 191. B 197. D 203. B 209. C 215. B 221. A 227. C 233. C 23 9. D 245. C 251. A 6. C 12. A 18. C 24. D 30. A 36. C 42. C 48. C 54. D 60. D 66 . A 72. A 78. D 84. A 90. C 96. A 102. A 108. A 114. A 120. C 126. A 132. B 138. B 144. B 150. D 156. C 162. C 168. C 174. D 180. C 186. D 192. C 198. C 204. D 210. D 216. B 222. D 228. C 234. A 240. D 246. B 252. C
276 253. D 259. D 265. C 271. C 277. A 283. C 289. D 295. C 301. B 307. D 313. A 319 . D 325. A 331. C 337. A 343. A 349. B 355. A 361. B 367. D 373. B 379. D 385. B 391. C 397. B 403. D 409. D 415. D 421. C 427. C 433. D 439. B 445. D 451. D 45 7. A 463. C 469. A 475. C 481. D 487. A 493. A 499. C 505. C 254. D 260. A 266. D 272. B 278. B 284. D 290. B 296. A 302. C 308. B 314. B 320. A 326. D 332. B 3 38. C 344. A 350. D 356. B 362. A 368. D 374. D 380. B 386. B 392. D 398. B 404. A 410. A 416. B 422. D 428. C 434. C 440. C 446. D 452. B 458. D 464. B 470. A 476. D 482. D 488. B 494. D 500. C 506. C 255. C 261. B 267. B 273. A 279. C 285 . C 291. B 297. D 303. B 309. B 315. B 321. C 327. A 333. A 339. C 345. B 351. A 357. D 363. A 369. B 375. C 381. D 387. C 393. A 399. C 405. D 411. C 417. C 42 3. D 429. D 435. C 441. C 447. D 453. D 459. B 465. A 471. B 477.A 483. C 489. D 495. B 501. A 507. A 256. C 262. B 268. C 274. C 280. C 286. B 292. C 298. C 30 4. A 310. C 316. A 322. C 328. B 334. D 340. C 346. D 352. B 358. A 364. C 370. B 376. B 382. D 388. D 394. C 400. A 406. C 412. B 418. A 424. D 430. C 436. B 4 42. A 448. C 454. D 460. A 466. C 472. D 478. A 484. C 490. A 496. D 502. B 508. C MCQ IN BIOCHEMISTRY 257. C 263. A 269. B 275. A 281. D 287. A 293. B 299. C 305. C 311. A 317. A 323 . C 329. C 335. D 341. C 347. C 353. D 359. D 365. C 371. B 377. B 383. D 389. C 395. D 401. D 407. D 413. A 419. D 425. C 431. B 437. D 443. C 449. A 455. B 46 1. C 467. B 473. D 479. C 485. B 491. B 497. C 503. D 509. C 258. D 264. A 270. D 276. C 282. D 288. A 294. A 300. D 306. D 312. C 318. D 324. C 330. D 336. C 3 42. D 348. B 354. C 360. B 366. C 372. C 378. D 384. C 390. D 396. A 402. B 408. C 414. C 420. C 426. C 432. C 438. C 444. C 450. D 456. C 462. D 468. D 474. C 480. C 486. D 492. B 498. D 504. D 510. B
NUCLEIC ACIDS 511. B 517. D 523. C 529. C 535. C 541. B 547. A 553. C 559. B 565. A 571. B 577 . A 583. C 589. B 595. C 601. B 607. B 613. D 619. A 512. A 518. C 524. B 530. B 536. C 542. B 548. B 554. A 560. C 566. C 572. A 578. A 584. D 590. B 596. B 60 2. B 608. D 614. C 620. D 513. D 519. B 525. C 531. D 537. C 543. C 549. B 555. D 561. C 567. C 573. A 579. D 585. B 591. D 597. D 603. D 609. B 615. C 621. C 5 14. B 520. B 526. C 532. A 538. B 544. D 550. C 556. B 562. A 568. C 574. C 580. B 586. D 592. B 598. B 604. A 610. A 616. B 515. D 521. B 527. C 533. A 539. D 545. A 551. C 557. C 563. A 569. D 575. C 581. C 587. A 593. B 599. A 605. B 611 . D 617. A 516. B 522. C 528. A 534. B 540. D 546. C 552. D 558. A 564. A 570. B 576. D 582. B 588. B 594. D 600. C 606. C 612. B 618. A 277
Thi
pag
int ntionally l ft
lank
CHAPTER 10 LECTROLYTE WATER & ELECTROLYTE BALANCE 1. Th total o y wat a p c ntag of th 70%
in va iou u j ct i lativ ly con tant wh n xp l an o y ma an i a out (A). 30% (C) 50% (B) 40% (D)
7. Th flui p nt in on which can not xchang a ily cau of la tiv ava cula ity i a out (A) 20 ml/kg (C) 45 ml/kg (B) 25 ml/kg (D) 60 ml/kg 2.. Th p c ntag of wat contain in th o y of an in ivi ual i l cau of (A) High fat cont nt (B) Low fat cont nt (C) High p ot in cont nt(D) Low p ot in cont nt 3. In int ac llula compa tm nt th flui p nt in ml/kg o y w ight i a out (A) 100 (C) 200 (B) 200 (D) 330 8. Wat iv in gm f om compl t oxi ation of ut (A) 0.15 (C) 0.35 (B) 0.25 (D) 0.55 9. Th oxi ation of 100 gm of fat yi l 7 gm wat (D) 200 gm wat 4. In xt a c llula compa tm nt, th out (A) 120 (C) 270 (B) 220 (D) 330
(A) 50 gm wat
flui
p
ti
u
an
in ml/kg
11. Th aily total o y wat iv f om oxi ation of foo ) 100 ml (C) 600 ml (B) 300 ml (D) 1000 ml
12. Th aily wat allowanc fo no mal infant i ml (B) 250–300 ml (D) 1000–2000 ml
ag
at
i
a o
(B) 10
o y w ight i
(A) 0.21 gm wat
ca tilag
6. Th total o y wat in ml/kg o y w ight in av i a out (A) 200 (C) 600 (B) 400 (D) 1000
ohy
(C) 150 gm wat
nt in ml/kg of
10. Each gm of p ot in on compl t oxi ation yi l m wat (B) 0.31 gm wat (D) 0.51 gm wat 5. Flui p nt in n conn ctiv a out (A) 10 (C) 45 (B) 20 (D) 55
ach gm of ca
tuff
a
(C) 0.41 g o y w ight i i
a out (A
no mal young a ult mal
a out (A) 100–200 ml (C) 330–1000
280 13. Th aily wat allowanc fo no mal a ult (60 kg) i 800–1500 ml (B) 500–800 ml (D) 1800–2500 ml
a out (A) 200–600 ml (C)
MCQ IN BIOCHEMISTRY 22. Minimum xc to y u ina y volum fo wa t p o uct limination i (A) 200–300 ml (C) 500–600 ml (B) 200–400 ml (D) 800 ml 14. In n i l lo of o y wat of no mal a ult i ml (B) 100–200 ml (D) 600–1000 ml
u ing 24 h
a out (A) 50–100 ml (C) 300–500
23. In p ima y hy ation (A) Int ac llula flui volum i uc (B) Int ac llula flui volum main no mal (C) Ext ac llula flui volum i much uc (D) Ext ac llula flui volum i much inc a 24. An impo tant cau of c on a y hy ation i (A) Dy phagia (B) O ophag al va ic (C) O ophag al va i c (D) Ga t o nt iti 25. Impo tant fin ing of con a y hy ation i (A) In t ac llula o ma (B) C llula hy ation (C) Thi t (D) Mu cl c amp 26. U i n xamination in con a y hy ation how (A) (B) (C) (D) K tonu ia Low p c ific g avity High p cific g avity Al uminu ia 15. Th +
p
ominant cation of pla ma i
(A) Na+ (C) Ca
(B) K + (D) Mg ++ 16. Th p ominant action of pla ma i (A) HCO3– (C) HPO4– – 17. Va op in (ADH) (A ) Enhanc facultativ a o ption of wat (B) D c a a o ption of wat (C) Inc a xc tion of calcium (D) D c a xc tion of calcium 18. Enhanc facultativ a o ption of wat y Va op in i m iat y (A) Cyclic AM P (C) Cyclic GMP (B) Ca (D) Mg ++ ++ (B) Cl– (D) SO4 – – 19. Action of kinin i to (A) (B) (C) (D) Inc a t ntion D c a wat t ntion Inc a oth 20. Th activity of kinin i a cAMP l v l (D) Inc a ation i (A) N ph og nic ia (D) Fanconi yn om 27. Th total calcium of th 0 g (D) 2–3 kg
alt
mo ulat y (A) P o taglan in (B) Ca++ (C) Inc cGMP l v l 21. An impo tant cau of wat intoxic t in ipi u (B) R nal failu (C) Ga t o nt iti human
28. Daily qui m nt of calcium fo ) 800 mg (D) 4 g 29. No mal total g (D) 50–100 mg
alt xc tion D c a alt an wat xc tion
o y i
a out (A) 100–150 g (C) 1–1.5 kg (B) 200–30
no mal a ult human i
um calcium l v l va i
tw
(A) 100 mg (C) 2 g (B
n (A) 4–5 mg (C) 15–20 mg (B) 9–11 m
WATER AND ELECTROLYTE BALANCE 30. Th l m nt n in quantiti g at Calcium (C) S l nium (B) Zinc (D) Co alt
than 100 mg fo
281 39. In um p o uct of Ca x p (in mg/100ml) in chil 50 (B) 30 (D) 60 31. Th min al p nt in th human o y in la g n i (A) So ium (C) Pota ium (B) Calcium (D) I on
n i
of th
total
o y calcium p
41. In man, th amount of calcium in gm glom uli i (A) 5 (C) 15 (B) 10 (D) 20 33. Th p 5 (D) 50
c ntag
42. Th p c ntag (D) 70–90
filt
of calcium p
nt in
of th
liminat
calcium
than any oth um i
nt in
on
in 24 h
xt ac llula in f c
flui i
ing
i
(A)
no mally (A) 20 (C)
amount
40. In ick t, th p o uct of Ca x p (in mg/ 100 ml) in ) 70 (B) 50 (D) 100 32. Th p c ntag ) 11 (D) 99
human
i p i
catio
low (A) 30 (C (A) 1 (C) 55 (B
io
y th
nal
(A) 1 (C) 10 (B)
(A) 10–20 (C) 50–60 (B) 30–40
34. Th phy iologically activ fo m of calcium i (A) (B) (C) (D) P ot in oni Compl x with cit at Compl x with ca onat
on
I
43. Th maximal nal tu ula a o ptiv capacity fo calcium (Tmca) in mg/min i a out (A) 1.5 0.1 (C) 5.5 1.2 (B) 4.99 0.21 (D) 10.2 2.2 35. Th no mal conc nt ation of calcium in C.S.F i ml 2.5–4 mg/100 ml 4.5–5 mg/100 ml 9–10 mg/100 ml
(A) (B) (C) (D) 1.5–2.5 mg/100
44. R nal ick t i cau y nal tu ula f ct (u ually inh it ) which int f with a o ption of (A) Calcium (C) So ium (B) Pho pho ou (D) Chlo i 36. A o ption of calcium i inc a on a (A) High p ot in i t (B) Low p ot i n i t (C) High fat i t (D) Low fat i t 37. Calcium a o ption i int f y (A) (B) (C) (D) P ot in in i t Phytic aci in c al Alkalin int tinal pH Vitamin D 45. Aft op ativ moval of th oi i m th conc nt ation of th (B) 10 mg (D) 7 mg 46. On of th (B) S (D) Co
p incipal cation
38. Calcium a o ption i (D) Vitamin E
inc
a
pa athy oi glan um calcium may
of
oft ti
u
an
op
ulting into hypopa athy low (A) 11 mg (C) 9 mg
o y flui
i
(A) Mg (C) Mn
y (A) Vitamin D (C) Vitamin K (B) Vitamin C
47. Th no mal conc nt ation of magn ium in whol 1–2 mg/100 ml (C) 2–4 mg/100 ml (D) 4–8 mg/100 ml
loo
i
(A) 0–1 mg/100 ml (B)
282 48. Th no mal conc nt ation of magn ium in C.S.F i 5 mg/100 ml (B) 3 mg/100 ml (D) 8 mg/100 ml
a out (A) 1 mg/100 ml (C)
MCQ IN BIOCHEMISTRY 57. Hyp nat mia may occu in (A) Dia t in ipi u (B) Diu tic m ) H avy w ating (D) Ki n y i a 58. Th m ta oli m of o ium i th ho mon : (A) In ulin (C) PTH (B) Al o t on (D) Somato tatin 49. Th magn ium cont nt of mu cl ) 10 mg/100 ml (D) 50 mg/100 ml
i
ication (C gulat y
a out (A) 5 mg/100 ml (C) 21 mg/100 ml (B
50. Int tinal a o ption of magn ium i inc a in (A) Calcium fici nt i t (B) High calcium i t (C) High oxalat i t (D) High phytat i t 51. D fici n cy of magn ium may occu with (A) Alcoholi m (B) Dia t m llitu (C) Hypothy oi i m (D) A vanc nal failu 52. Hyp magn mia may o v in (A) Hyp pa athy oi i m (B) Dia t m llitu (C) Kwa hio ka (D) P ima y al o t oni m 53. Na+/K+-ATPa along with ATP qui (A) Ca (C) Mg (B) Mn (D) Cl 59. Th p incipal cation in int ac llula ta ium (D) Magn ium
flui
i
(A) So ium (C) Calcium (B) Po
60. Th no mal conc nt ation of pota ium in whol loo i (A) 50 mg/100 ml (B) 100 mg/100 ml (C) 150 mg/100 ml (D) 200 mg/100 ml 61. Th no mal conc nt ation of pota ium in human pla ma in m q/I i a out (A) 1 (C) 3 (B) 2 (D) 5 62. Th no mal conc nt ation of pota (C) 350 (B) 200 (D) 440 54. Th p incipal cation in ta ium (D) Magn ium 63. Pota ium cont nt of n 330 (D) 530
ium in c ll
xt ac llula v
ti
55. Th no mal conc nt ation of (C) 250 (B) 200 (D) 330 64. Pota ium cont nt of mu cl (B) 100–150 (D) 150–200
u
flui
i
in ng/100 ml i
a out (A) 100
(A) So ium (C) Calcium (B) Po
in mg/ 100 ml i
a out (A) 200 (C) 400 (B)
o ium (in mg/100 ml) of human pla ma i ti
u
in mg/100 ml i
(A) 100
a out (A) 50–100 (C) 250–400
56. A c a in um o ium may occu in (A) A noco tical in uffici ncy (B) Hypopa athy oi i m (C) Hyp pa athy oi i m (D) Thy otoxico i 65. On w akn
of th ymptom of low um pota ium conc nt ation inclu (B) Confu ion (C) Num n (D) Tingling of xt miti
(A) Mu cl
WATER AND ELECTROLYTE BALANCE 66. Pota ium m ta oli m i gulat tatin (B) PTH (D) E t og n
y th
ho mon : (A) Al o t
on
(C) Somato
283 75. Th xclu iv function of i on in th o y i confin to th p oc of (A) Mu cula cont action (B) N v xcitation (C) C llula pi ation (D) Bloo co agulation 76. Th no mal pH of th loo i (A) 7.0 (C) 7.2 (B) 7.1 (D) 7.4 67. A high um pota ium, accompani y a high int ac llula pota ium occu in (A) A nal in uffici ncy (B) Any illn (C) Ga t oint tinal lo (D) Cu hing’ yn om 68. Hypokal mia occu in (A) Cu hing’ yn om (B) A i on’ i (C) R nal failu (D) A vanc hy ation 69. Ca iac a t may occu u to ov o of (A) So ium (C) Zinc (B) Pota ium (D) Magn ium 77. Th no mal conc nt ation of L (B) 24 m q/L (D) 30 m q/L
ica
onat
in
loo
i
a
(A) 21 m q/L (C) 26 m q/
78. At th pH of loo 7.4, th atio tw n th ca onic aci f action i (A) 1 : 10 (C) 1 : 30 (B) 1 : 20 (D) 1 : 40
an
ica
70. Th no mal conc nt ation of chlo i ) 200 (C) 400 (B) 250 (D) 450
loo
i
in mg/100 ml of whol
onat
a out (A
79. A 0.22 M olution of lactic aci (pKa 3.9) wa foun to contain 0.20 M in th i ociat fo m an 0.02 M un i ociat fo m, th pH of th olution i (A) 2.9 (C) 4.9 (B) 3.3 (D) 5.4 71. Th no mal conc nt ation of chlo i (C) 365 (B) 200 (D) 450
in mg/100 ml of pla ma i
a out (A) 100
80. Impo tant uff y t m of xt ac llula flui i (A) Bica onat /ca onic a ci (B) Di o ium hy og n pho phat / o ium ihy og n pho phat (C) Pla ma p ot in (D) O ganic Pho phat 81. Th pH of o y flui i ta iliz y uff y t m . Th compoun which will th mo t ff ctiv uff at phy iologic pH i ( A) Na2HPO4 pKa = 12.32 (B) Na2HPO4 pKa=7.21 (C) NH4OH pKa = 7.24 (D) Cit ic aci pKa = 3.09 72. Th no mal conc nt ation of chlo in (C) 300 (B) 250 (D) 440
in mg/100 ml of C.S.F i
a out (A) 200
73. Hypokal mia with an accompanying hypochlo mic alkalo i may o v in (A) Cu hing’ yn om (B) A i on’ i a (C) Hyptothy oi i m (D) Malnut ition 74. Hyp chol mia i a ociat with (A) Hyponat mia (B) Hyp nat mia (C) M ta o lic alkalo i (D) R pi ato y aci o i
284 82. Th p c ntag of CO2 ca ying capacity of whol h moglo in i (A) 20 (C) 60 (B) 40 (D) 80 MCQ IN BIOCHEMISTRY 90. Of th total o y wat ) 70% (B) 60% (D) 80% 83. Th no mal ) 35–38 m q/L
, int ac llula
um CO2 cont nt i
91. O motically activ i (D) All of th
u
tanc
loo
y h moglo in an
compa tm nt contain
oxy
a out (A) 50% (C
(A) 18–20 m q/L (C) 30–34 m q/L (B) 24–29 m q/L (D
in pla ma a
84. Th ca on ioxi ca ying pow i (A) 50% (C) 85% (B) 60% (D) 100%
of th
(A) So ium (C) P ot in
loo
(B) Chlo
i ing within th
c ll
92. O motic p u of pla ma i (A) 80–100 millio mol /lit (B) 180–200 millio mo l /lit (C) 280–300 millio mol /lit (D) 380–400 millio mol /lit 93. Cont i utio n of al umin to colloi o motic p u of pla ma i a out (A) 10% (C) 80% (B) 50% (D) 90% 85. Within th loo c ll th uff t i (A) 5% (C) 20% (B) 10% (D) 25%
ing capacity cont i ut
86. Th no mal atio tw n th alkalin pho phat i (A) 2 : 1 (C) 20 : 1 (B) 1 : 4 (D) 4 : 1
an
aci
y th
pho phat
pho pha
in pla ma
94. Th high t conc nt ation of p ot in i p nt in (A) Pla ma (C) Int ial flui (B) Int titial flui (D) T an c llula flui 87. Th oxyg n i ociation cu v fo (B) (C) (D) D c a O2 t n ion D c a pH
h moglo in i hift a CO2 t n ion Inc
95. Oncotic p ) All of th
to (A) P ot in
u
of pla ma i
96. Oncotic p u of pla ma i m of Hg (D) 50 mm of Hg
u
tit
to th ight y (A) a CO2 t n ion Inc
(C) So ium (B) Chlo i
(D
a out (A) 10 mm of Hg (C) 25 mm of Hg (B) 15 m
88. Boh ff ct i (A) Shifting of oxyh moglo in i ociation cu v to th ight (B) Shifting of oxyh moglo in i ociation cu v to th l ft (C) A ility of h m oglo in to com in with O2 (D) Exchang of chlo i with ca onat 89. Chlo i hift i (A) H ion l aving th RBC in xchang of Cl(B) Cl– l aving th RBC in x chang of ica onat (C) Bica onat ion tu n to pla ma an xchang with c hlo i which hift into th c ll (D) Ca onic aci to th pla ma 97. O ma can occu wh n (A) Pla ma Na an Cl a c a (B) Pla ma Na an C l a inc a (C) Pla ma p ot in a c a (D) Pla ma p ot in a inc a 98. Colloi o motic p u of int ac llula flui i (A) Equal to that of pla ma (B) Mo than that of pla ma (C) Mo than that of pla ma (D) N a ly z o
WATER AND ELECTROLYTE BALANCE 99. Th wat p o uc u ing m ta olic action in an a ult i l/ ay (C) 500 ml/ ay (B) 300 ml/ ay (D) 700 ml/ ay
a out (A) 100 m
285 107. Fu o mi inhi it a o ption of o ium an chlo i in (A) P oximal con volut tu ul (B) Loop of H nl (C) Di tal convolut tu ul (D) Coll cting uct 108. A iu tic which i an al o t on antagoni t i (A) Spi onolacton (C ) Ac tazolami (B) Ethac ynic aci (D) Chlo othiazi 100. Th aily wat lo th ough ga t oint tinal t act in an a ult i ) (B) (C) (D) L than 100 ml/ ay 200 ml/ ay 300 ml/ ay 400 ml/ ay 101. R cu nt vomiting l a i (D) All of th
to lo
of (A) Pota
ium (C) Bica
onat
a out (A (B) Chlo
109. In a olution having a pH of 7.4, th hy og n ion conc nt ation i nmol/L (C) 56 nmol/L (B) 40 nmol/L (D) 80 nmol/L
(A) 7.4
102. O ligato y a o ption of wat (A) I a out 50% of th total tu ula a o ption of wat (B) I inc a y anti iu tic ho mon (C) Occu in i tal convolut tu ul (D) I con a y to a o ption of olut 103. Anti iu tic ho mon (A) I c t y hypothalamu (B) S c tion i inc a wh n o molal ity of pla ma c a (C) Inc a o ligato y a o ption of wat (D) Act on i tal convolut tu ul an coll cting uct 104. U ina y wat lo i inc a in (A) (B) (C) (D) Dia t m llitu Dia t in ipi u Ch onic glom ulo n ph iti All of th 110. At pH 7.4, th atio of (B) 10 : 1 (D) 40 : 1 111. Quantitativ t uff y t m tat uff y t hat uff , th og n pho phat i 105. Dia D c a o c pto
ica
onat
:
i
olv
CO2 i
(A) 1 : 1 (C) 20 : 1
ly, th mo t ignificant uff y t m in pla ma i (A) Pho pha (B) Ca onic aci - ica onat uff y t m (C) Lactic aci -lac m (D) P ot in uff y t m 112. In a olution containing pho p pH will 7.4, if th atio of monohy og n pho phat : ihy (A) 4 : 1 (C) 10 : 1 (B) 5 : 1 (D) 20 : 1
t in ipi u ult f om (A) (B) (C) (D) D c a ADH c tion D c a al o t on c tion Un
113. pKa of 106. Thiazi c tion ADH
ihy
og n pho phat
i
in ulin pon iv n
c tion of o m
(A) 5.8 (C) 6.8 (B) 6.1 (D) 7.1
iu tic inhi it (A) (B) (C) (D) Ca onic anhy c tion So ium a o ption in i tal tu ul
114. Buff ing action of ha moglo in i (B) A ginin i u (C) Hi ti in
mainly u to it i u (D) Ly in
a
Al o t
(A) Glutamin i u
on i u
286 115. R pi ato y aci o i ult f om (A) (B) (C) (D) R t ntion of ca on ioxi Exc iv limination of ca on ioxi R t ntion of ica onat Exc iv l imination of ica onat MCQ IN BIOCHEMISTRY 123. Anion gap i inc a in (A) R nal tu ula aci o i (B) M ta olic aci o i ulting f om ia ho a (C) M ta olic aci o i ulting f om int tinal o t uction (D) Dia tic k toaci o i 124. Anion gap in pla ma i cau (A) Of iff ntial i t i ution of ion ac o c ll m m an (B) Cation outnum anion in pla ma (C) Anion outnum cation in pla ma (D) Of unm a u anion in pl a ma 125. Salicylat poi oning can cau (A) (B) (C) (D) R pi ato y aci o i M ta olic aci o i with no mal anion gap M ta olic aci o i with inc a anion g ap M ta olic alkalo i 116. R pi ato y aci o i can occu in all of th following xc pt (A) (B) (C) ( D) (A) (B) (C) (D) (A) (B) (C) (D) Pulmona y o ma Hy t ical hyp v ntilation Pn umotho ax Emphy ma D c a in pH Inc a in pCO2 Inc a in pla ma ica onat D c a in pla ma ica onat B onchial a thma Collap of lung Hy t ic al hyp v ntilation B onchial o t uction 117. Th 118. R
initial
v nt in
pi ato y alkalo i
pi ato y aci o i can occu
i
in
126. Anion gap of pla ma can u to th p nc of all th (A) Bica onat (C) Py uvat (B) Lactat (D) Cit at
following
xc pt
119. Th p ima y v nt in pi ato y alkalo i i c a in pCO2 Inc a in pla ma ica onat D c
(A) (B) (C) (D) Ri in pH D a in pla ma chlo i
127. All th following f atu a foun in loo ctic aci o i xc pt (A) (B) (C) (D) pH i c a O2 i no mal Anion gap i no mal
ch mi t y in uncomp n at Bica onat i c a
la pC
120. Anion gap i th iff nc in th pla ma conc nt ation of (A) (Chlo i ) – (Bica onat ) (B) (So ium) – (Chlo i ) (C) (So ium + Pota ium) – (Chlo i + Bica onat ) (D) (Sum of cation ) – (Sum of anion ) 121. No mal anion gap in pla ma i a out (A) 5 m q/L (C) 25 m q/L (A) (B) (C) (D) (B) 15 m q/L (D) 40 m q/L 128. All th following tat m nt a out nal tu ula aci o i a co ct xc p t (A) R nal tu ul may una l to a o ica onat (B) R nal tu ul may una l to c t hy og n ion (C) Pla ma chlo i i l vat (D) Anion gap i c a 129. All th following chang in loo ch mi t y can occu in v ia ho a xc pt (A) (B) (C) (D) D c a pH D c a ica onat Inc a pCO2 Inc a chlo i 122. Anion gap i no mal in Hyp chlo a mic m ta olic aci o i o i Lactic aci o i U a mic aci o i
Dia
tic k toaci
WATER AND ELECTROLYTE BALANCE 130. Du ing comp n ation of pi ato y alkalo i , all th following chang occ u xc pt (A) D c a c tion of hy og n ion y nal tu ul (B) Inc a xc tion of o ium in u in (C) Inc a xc tion of ica onat in u in ( D) Inc a xc tion of ammonia in u in 131. Bloo ch mi t y how th follow ing chang in comp n at pi ato y aci o i : (A) (B) (C) (D) Inc a pCO2 Inc a ica onat D c a chlo i All of th 287 (C) Mov of 1 kg l cula
a ma y 1 m iz i
of 1 gm y 1 cm i tanc y a fo c of 1 N wton (D) Mov i tanc y a fo c of 1 N wton 135. O ganic compoun of (A) U a (C) C atinin (B) U ic aci (D) Pho phat
136. O ganic u lin (D) P ot in 137. Bo y wat pin ph in
tanc i
of la g
gulat
mol cula
y th
iz
i
(A) Sta ch (C) Lipi
ia ho a R nal fai inhi ito
th activation of th nzym : (A) (B) (C) (D) I oci Succinat thiokina ATPa
133. Which of th following f atu a p nt in loo at m ta olic alkalo i xc pt? (A) (B) (C) (D) Inc a at No mal chlo i No mal pCO2 139. Co alt i otin
(B) In u
ho mon : (A) Oxytocin (C) FSH (B) ACTH (D) E
132. M ta olic alkalo i can occu in (A) (B) (C) (D) S v lu R cu nt vomiting Exc iv u of ca onic anhy a 138. Calcium i qui fo t at hy og na Fuma a
a ma mall mo
a con titu nt of (A) Folic aci
ch mi t y in uncomp n pH Inc a ica on
(C) Niacin (B) Vitamin B12 (D) Bi
140. Calcium a o ption i inf y (A) Fatty aci ci (D) Vitamin B12 (B) 6.0 (D) 7.0
(C) Vitamin D (B) Amino a
134. On joul i th n gy t y 1°C (B) Rai th t mp
t mp atu y 1°C
141. Th
av
ag
of pH of u in
qui atu i
to (A) Rai th of 1 kg of wat (A) 5.6 (C) 6.4
of 1 gm of wa
288 MCQ
IN BIOCHEMISTRY
ANSWERS 1. D 7. C 13. D 19. D 25. A 31. B 37. B 43. B 49. C 55. D 61. D 67. A 73. A 79. C 85. D 91. D 97. C 103. D 109. B 115. A 121. B 127. D 133. D 139. B 2. A 8. D 1 4. D 20. A 26. B 32. D 38. A 44. B 50. A 56. A 62. D 68. A 74. B 80. A 86. D 92. C 98. B 104. D 110. C 116. B 122. A 128. D 134. D 140. A 3. D 9. B 15. A 21. B 27. C 33. A 39. C 45. D 51. A 57. A 63. D 69. B 75. C 81. B 87. C 93. C 99. B 10 5. B 111. B 117. B 123. B 129. C 135. A 141. B 4. C 10. C 16. B 22. C 28. B 34. B 40. A 46. A 52. B 58. B 64. C 70. B 76. D 82. C 88. A 94. C 100. A 106. D 112. A 118. C 124. B 130. D 136. D 5. C 11. B 17. A 23. A 29. B 35. C 41. B 47. C 53 . C 59. B 65. A 71. C 77. C 83. B 89. C 95. A 101. B 107. B 113. C 119. B 125. C 131. D 137. A 6. C 12. C 18. A 24. D 30. A 36. A 42. D 48. B 54. A 60. D 66. A 72. D 78. B 84. C 90. C 96. C 102. D 108. A 114. C 120. C 126. A 132. C 138. D
